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https://www.readbyqxmd.com/read/29049953/biomimetic-fabrication-of-antibacterial-calcium-phosphates-mediated-by-polydopamine
#1
Lucia Forte, Paola Torricelli, Francesca Bonvicini, Elisa Boanini, Giovanna Angela Gentilomi, Gigliola Lusvardi, Elena Della Bella, Milena Fini, Edoardo Vecchio Nepita, Adriana Bigi
In this work we developed new antibacterial composite materials using polydopamine (PDA) to trigger the deposition of silver nanoparticles (AgNPs) onto calcium phosphates, namely octacalcium phosphate (OCP) and α-tricalcium phosphate (αTCP). Functionalization of OCP and αTCP with a self-polymerized polydopamine layer was obtained by soaking the calcium phosphates in dopamine solution. The PDA surface of functionalized calcium phosphates (OCPd and αTCPd) promoted the deposition of AgNPs by reducing silver ions when soaked in a silver nitrate solution...
October 10, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29049377/truncated-hemoglobin-1-is-a-new-player-in-chlamydomonas-reinhardtii-acclimation-to-sulfur-deprivation
#2
Ekaterina Minaeva, Zhanneta Zalutskaya, Valentina Filina, Elena Ermilova
Truncated hemoglobins constitute a large family, present in bacteria, in archaea and in eukaryotes. However, a majority of physiological functions of these proteins remains to be elucidated. Identification and characterization of a novel role of truncated hemoglobins in the model alga provides a framework for a more complete understanding of their biological functions. Here, we use quantitative RT-PCR to show that three truncated hemoglobins of Chlamydomonas reinhardtii, THB1, THB2 and THB12, are induced under conditions of depleted sulfur (S) supply...
2017: PloS One
https://www.readbyqxmd.com/read/29048563/linear-discriminant-analysis-reveals-differences-in-root-architecture-in-wheat-seedlings-related-to-nitrogen-uptake-efficiency
#3
Kim Kenobi, Jonathan A Atkinson, Darren M Wells, Oorbessy Gaju, Jayalath G De Silva, M John Foulkes, Ian L Dryden, Andrew T A Wood, Malcolm J Bennett, Miriam Gifford
Root architecture impacts water and nutrient uptake efficiency. Identifying exactly which root architectural properties influence these agronomic traits can prove challenging. In this paper, approximately 300 wheat (Triticum aestivum) plants were divided into four groups using two binary classifications, high versus low nitrogen uptake efficiency (NUpE), and high versus low nitrate in the growth medium. The root system architecture for each wheat plant was captured using 16 quantitative variables. The multivariate analysis tool, linear discriminant analysis, was used to construct composite variables, each a linear combination of the original variables, such that the score of the plants on the new variables showed the maximum between-group variability...
October 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29048154/hexanuclear-and-trinuclear-metal-complexes-of-a-giant-octadecaaza-macrocycle
#4
Janusz Gregoliński, Katarzyna Ślepokura, Jerzy Lisowski
A large macrocyclic ligand containing six pyridine fragments and six diaminocyclopentane fragments is able to form hexanuclear Zn(II) and Ni(II) complexes as well as a trinuclear Zn(II) complex. X-ray crystal structures of these complexes indicate quite different ligand conformations. In the hexanuclear Zn(II) derivative with chloride counteranions metal ions have a distorted-trigonal-bipyramidal geometry and occupy loop sections formed by the highly folded macrocycle, which adopts a globular shape. In the hexanuclear Ni(II) derivative with nitrate counteranions metal ions exhibit a distorted-octahedral geometry and the ligand conformation is much more open, while in the trinuclear Zn(II) complex the macrocycle wraps around the octahedral metal ions...
October 19, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29047079/molecular-basis-of-nitrative-stress-in-the-pathogenesis-of-pulmonary-hypertension
#5
Colin E Evans, You-Yang Zhao
Pulmonary hypertension (PH) is a lung vascular disease with marked increases in pulmonary vascular resistance and pulmonary artery pressure (>25 mmHg at rest). In PH patients, increases in pulmonary vascular resistance lead to impaired cardiac output and reduced exercise tolerance. If untreated, PH progresses to right heart failure and premature lethality. The mechanisms that control the pathogenesis of PH are incompletely understood, but evidence from human and animal studies implicate nitrative stress in the development of PH...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29046722/replacing-process-water-and-nitrogen-sources-with-biogas-slurry-during-cellulosic-ethanol-production
#6
Yang You, Bo Wu, Yi-Wei Yang, Yan-Wei Wang, Song Liu, Qi-Li Zhu, Han Qin, Fu-Rong Tan, Zhi-Yong Ruan, Ke-Dong Ma, Li-Chun Dai, Min Zhang, Guo-Quan Hu, Ming-Xiong He
BACKGROUND: Environmental issues, such as the fossil energy crisis, have resulted in increased public attention to use bioethanol as an alternative renewable energy. For ethanol production, water and nutrient consumption has become increasingly important factors being considered by the bioethanol industry as reducing the consumption of these resources would decrease the overall cost of ethanol production. Biogas slurry contains not only large amounts of wastewater, but also the nutrients required for microbial growth, e...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29046317/vasoactivity-of-nitrite-in-the-iliac-artery-of-the-toad-rhinella-marina
#7
Leonard George Forgan, Melenaite Sofele, Bryony Alice McNeill, Melissa S Cameron, John A Donald
Nitrite (NO2(-)) causes vasodilation in mammals due to the formation of (nitric oxide) NO by endogenous NO2(-) reduction in the vascular wall. In this study, we determined if a similar mechanism operates in amphibians. Dual-wire myography of the iliac artery from Rhinella marina showed that applied NO2(-) caused a concentration-dependent vasodilation in normoxia (21% O2; EC50 438 µM). Hypoxia (0.63% O2) significantly increased the maximal dilation to NO2(-) by 5 % (P = 0.0398). The addition of oxyhemoglobin significantly increased the EC50 (P = 0...
October 18, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29046169/response-of-epiphytic-algae-to-heavy-pollution-of-water-bodies
#8
(no author information available yet)
The objective of the present work was to study the structure of epiphyton in water bodies significantly differing in the degree of their contamination. In the studied water bodies, the concentration of ammonia (NH4+-N) accounted for 0.04-74.00 mg/L, nitrite (NO2--N) - 0.002-1.750 mg/L, nitrate (NO3--N) - 0.13-58.00 mg/L, inorganic compounds of phosphorus (Pinorg) - 0.041-0.272 mg/L, chloride (Cl-) - 34.5-560.5 mg/L, whereas the content of organic matter (DO) - 18.0-81.0 mg O/L. The numbers of algae were determined by the method of a direct count...
October 17, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/29046002/-optimal-diagnostics-and-therapy-for-microvascular-angina-pectoris
#9
Peter Ong, Udo Sechtem
Patients with microvascular angina are characterized by angina pectoris with proof of myocardial ischemia in the absence of any relevant epicardial stenosis and without myocardial disease (type 1 coronary microvascular dysfunction according to Crea and Camici). Structural and functional alterations of the coronary microvessels (diameter < 500 µm) are the reason for this phenomenon. Frequently such alterations are associated with cardiovascular risk factors. Patients with angina pectoris without epicardial stenoses represent for 10 - 50 % of all patients undergoing coronary angiography depending on the clinical presentation...
October 2017: Deutsche Medizinische Wochenschrift
https://www.readbyqxmd.com/read/29045134/effects-of-the-ph-on-the-formation-and-doping-mechanisms-of-zno-nanowires-using-aluminum-nitrate-and-ammonia
#10
Claire Verrier, Estelle Appert, Odette Chaix-Pluchery, Laetitia Rapenne, Quentin Rafhay, Anne Kaminski-Cachopo, Vincent Consonni
The elucidation of the fundamental processes in aqueous solution during the chemical bath deposition of ZnO nanowires (NWs) using zinc nitrate and hexamethylenetetramine is of great significance: however, their extrinsic doping by foreign elements for monitoring their optical and electrical properties is still challenging. By combining thermodynamic simulations yielding theoretical solubility plots and speciation diagrams with in situ pH measurements and structural, chemical, and optical analyses, we report an in-depth understanding of the pH effects on the formation and aluminum doping mechanisms of ZnO NWs...
October 18, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29044840/impacts-of-climate-and-land-use-on-n2-o-and-ch4-fluxes-from-tropical-ecosystems-in-the-mt-kilimanjaro-region-tanzania
#11
Adrian Gütlein, Friederike Gerschlauer, Imani Kikoti, Ralf Kiese
In this study, we quantify the impacts of climate and land use on soil N2 O and CH4 fluxes from tropical forest, agroforest, arable and savanna ecosystems in Africa. To do so, we measured GHG fluxes from twelve different ecosystems along climate and land-use gradients at Mt. Kilimanjaro, combining long-term in situ chamber and laboratory soil-core incubation techniques. Both methods showed similar patterns of GHG exchange. Although there were distinct differences from ecosystem to ecosystem, soils generally functioned as net sources and sinks for N2 O and CH4 , respectively...
October 17, 2017: Global Change Biology
https://www.readbyqxmd.com/read/29044545/predicted-global-warming-scenarios-impact-on-the-mother-plant-to-alter-seed-dormancy-and-germination-behavior-in-arabidopsis
#12
Z Huang, S Footitt, A Tang, W E Finch-Savage
Seed characteristics are key components of plant fitness that are influenced by temperature in their maternal environment, and temperature will change with global warming. To study the effect of such temperature changes, Arabidopsis thaliana plants were grown to produce seeds along a uniquely designed polyethylene tunnel having a thermal gradient reflecting local global warming predictions. Plants therefore experienced the same variations in temperature and light conditions, but different mean temperatures...
October 17, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29043485/nitrate-assimilation-pathway-nap-role-of-structural-nit-and-transporter-ntr1-genes-in-fusarium-oxysporum-f-sp-lycopersici-growth-and-pathogenicity
#13
Lucia Gomez-Gil, Jesus Camara Almiron, Patricia Lizett Rodriguez Carrillo, Cindy Nayely Olivares Medina, Gustavo Bravo Ruiz, Pamela Romo Rodriguez, Alma Rosa Corrales Escobosa, Felix Gutierrez Corona, M Isabel Roncero
The tomato pathogen Fusarium oxysporum f.sp. lycopersici possesses the capability to use nitrate as the only nitrogen source under aerobic and anaerobic conditions and to activate virulence-related functions when cultivated in the presence of nitrate, but not in ammonium. The genome of F. oxysporum f.sp. lycopersici encodes three paralogs nitrate reductase (NR) genes (nit1, nit2 and nit3) and one predicted ortholog of the Aspergillus nidulans high-affinity nitrate/nitrite transporters NtrA and NtrB, named ntr1...
October 17, 2017: Current Genetics
https://www.readbyqxmd.com/read/29043098/spermidine-is-protective-against-kidney-ischemia-and-reperfusion-injury-through-inhibiting-dna-nitration-and-parp1-activation
#14
Jinu Kim
Kidney ischemia and reperfusion injury (IRI) is associated with a high mortality rate, which is attributed to tubular oxidative and nitrative stresses; however, an effective approach to limit IRI remains elusive. Spermidine, a naturally occurring polyamine, protects yeast cells against aging through the inhibition of oxidative stress and necrosis. In the present study, spermidine supplementation markedly attenuated histological damage and kidney dysfunction during IRI. In addition, exogenous spermidine potently inhibited poly(ADP-ribose) polymerase 1 (PARP1) activation and DNA nitrative/oxidative stress following IRI...
September 2017: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/29042862/highly-efficient-single-step-enrichment-of-low-abundance-phosphopeptides-from-plant-membrane-preparations
#15
Xu Na Wu, Lin Xi, Heidi Pertl-Obermeyer, Zhi Li, Liang-Cui Chu, Waltraud X Schulze
Mass spectrometry (MS)-based large scale phosphoproteomics has facilitated the investigation of plant phosphorylation dynamics on a system-wide scale. However, generating large scale data sets for membrane phosphoproteins usually requires fractionation of samples and extended hands-on laboratory time. To overcome these limitations, we developed "ShortPhos," an efficient and simple phosphoproteomics protocol optimized for research on plant membrane proteins. The optimized workflow allows fast and efficient identification and quantification of phosphopeptides, even from small amounts of starting plant materials...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29042272/effect-of-dietary-nitrate-on-respiratory-physiology-at-high-altitude-results-from-the-xtreme-alps-study
#16
Andrew Cumpstey, Philip J Hennis, Edward T Gilbert-Kawai, Bernadette O Fernandez, Matthieu Poudevigne, Alexandra Cobb, Paula Meale, Kay Mitchell, Helen Moyses, Helmut Pöhnl, Monty G Mythen, Michael P W Grocott, Martin Feelisch, Daniel S Martin
Nitric oxide (NO) production plays a central role in conferring tolerance to hypoxia. Tibetan highlanders, successful high-altitude dwellers for millennia, have higher circulating nitrate and exhaled NO (ENO) levels than native lowlanders. Since nitrate itself can reduce the oxygen cost of exercise in normoxia it may confer additional benefits at high altitude. Xtreme Alps was a double-blinded randomised placebo-controlled trial to investigate how dietary nitrate supplementation affects physiological responses to hypoxia in 28 healthy adult volunteers resident at 4559 m for 1 week; 14 receiving a beetroot-based high-nitrate supplement and 14 receiving a low-nitrate 'placebo' of matching appearance/taste...
October 14, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29040901/high-efficiency-stratification-of-apple-cultivar-ligol-seed-dormancy-by-phytohormones-heat-shock-and-pulsed-radio-frequency
#17
Mieczysław Grzesik, Krzysztof Górnik, Regina Janas, Mariusz Lewandowki, Zdzislawa Romanowska-Duda, Bert van Duijn
The aim of the study was to improve the effect of stratification of apple "Ligol" seeds by application of selected compounds, phytohormones, and physical methods For this purpose the seeds were stratified at 3°C in distilled water or in the presence of potassium nitrate (KNO3), ethephon (ET), carbon monoxide (CO), hydrogen peroxide (H2O2), a mixture of KNO3, ET, CO, H2O2, gibberellins (GA3), 6-benzylaminopurine (BAP), jasmonic acid (JA), salicylic acid (SA) and a mixture of SA, GA3, BAP, JA, nitric oxide (NO), hydrogen chloride (HCL)...
September 28, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29039945/structural-consequences-of-1-4-cyclohexanedicarboxylate-cis-trans-isomerism-in-uranyl-ion-complexes-from-molecular-species-to-2d-and-3d-entangled-nets
#18
Pierre Thuéry, Jack Harrowfield
trans-1,4-Cyclohexanedicarboxylic acid (t-1,4-chdcH2) or the commercially available mixture of the cis and trans isomers (c,t-1,4-chdcH2) has been used in the synthesis of a series of 14 uranyl ion complexes, all obtained under solvohydrothermal conditions, some in the presence of additional metal cations and/or 2,2'-bipyridine (bipy). With its two isomeric forms having very different shapes and its great sensitivity to the experimental conditions, 1,4-chdc(2-) appears to be suitable for the synthesis of uranyl ion complexes displaying a wide range of architectures...
October 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29039933/porous-uranium-diphosphonate-frameworks-with-trinuclear-units-templated-by-organic-ammonium-hydrolyzed-from-amine-solvents
#19
Zhi-Hui Zhang, Ganna A Senchyk, Yi Liu, Tyler Spano-Franco, Jennifer E S Szymanowski, Peter C Burns
By varying solvent systems, the solvothermal treatment of uranyl nitrate and methylenediphosphonic acid (H4PCP) afforded three new porous uranyl-organic frameworks (UOFs). All were structurally characterized by single-crystal X-ray diffraction and formulated as (Et2NH2)2[(UO2)3(PCP)2](H2O)2.5 (1), (MeNH3)(H3O)[(UO2)3(PCP)2(H2O)3] (2), and [Na(H2O)4](H3O)[(UO2)3(PCP)2(H2O)2](H2O)5 (3). These compounds crystallize with three-dimensional anionic frameworks containing U(VI) and distinct cationic species due to in situ solvent hydrolysis...
October 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29039826/-endothelial-immune-activation-and-functional-state-in-patients-with-hypertensive-disease
#20
T V Ashcheulova, O N Kovalyova, N N Gerasimchuk
AIM: To investigate the nitric oxide (NO) system, the activity of endothelial NO synthase (e-NOS) and inducible NO synthase (i-NOS) in relation to the levels of tumor necrosis factor-α (TNF- α) and its soluble receptor type I (sTNFRI) depending on the grade of hypertensive disease (HD). SUBJECTS AND METHODS: A total of 317 patients, including 284 patients aged 30 to 65 years (mean age 54.7±0.58 years) with Grades 1-3 HD who had not previously received regular antihypertensive therapy and 33 apparently healthy individuals, were examined at Kharkov City Clinical Hospital Eleven...
2017: Terapevticheskiĭ Arkhiv
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