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hermetia illucens

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https://www.readbyqxmd.com/read/28105887/light-microscopy-with-a-differential-staining-technique-for-the-characterization-and-discrimination-of-insects-versus-marine-arthropods-in-processed-animal-proteins
#1
Matteo Ottoboni, Marco Tretola, Federica Cheli, Daniela Marchis, Pascal Veys, Vincent Baeten, Luciano Pinotti
The aim of this study was to evaluate the use of light microscopy with a differential staining technique for the discrimination of insect material from marine arthropods - classified as fishmeal. Specifically, three samples of single species insect material, Hermetia illucens (HI), Bombyx mori (BM) and Tenebrio molitor (TM) and two samples of marine arthropods, shrimp material and krill, have been analysed and compared after staining by two reagents to enhance fragment identification. Alizarin Red (AR) and Chlorazol black (CB), which react respectively with calcium salts and chitin, were tested for their potential efficacy in distinguishing between insect and marine materials...
January 20, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28056070/screening-expression-purification-and-functional-characterization-of-novel-antimicrobial-peptide-genes-from-hermetia-illucens-l
#2
Osama Elhag, Dingzhong Zhou, Qi Song, Abdul Aziz Soomro, Minmin Cai, Longyu Zheng, Ziniu Yu, Jibin Zhang
Antimicrobial peptides from a wide spectrum of insects possess potent microbicidal properties against microbial-related diseases. In this study, seven new gene fragments of three types of antimicrobial peptides were obtained from Hermetia illucens (L), and were named cecropinZ1, sarcotoxin1, sarcotoxin (2a), sarcotoxin (2b), sarcotoxin3, stomoxynZH1, and stomoxynZH1(a). Among these genes, a 189-basepair gene (stomoxynZH1) was cloned into the pET32a expression vector and expressed in the Escherichia coli as a fusion protein with thioredoxin...
2017: PloS One
https://www.readbyqxmd.com/read/28054035/recovery-and-techno-functionality-of-flours-and-proteins-from-two-edible-insect-species-meal-worm-tenebrio-molitor-and-black-soldier-fly-hermetia-illucens-larvae
#3
Sara Bußler, Birgit A Rumpold, Elisabeth Jander, Harshadrai M Rawel, Oliver K Schlüter
Depending on the species, edible insects are highly nutritious and thus represent a noteworthy alternative food and feed source. The current work investigates the protein extractability and techno-functionality of insect flour fractions recovered from Tenebrio molitor and Hermetia illucens. T. molitor and H. illucens flours contained about 20% crude fat and 60% and 36 % crude protein, respectively. Defatting reduced the crude fat content to 2.8% (T. molitor) and 8.8% (H. illucens) and increased the crude protein content to 68% and 47%, respectively...
December 2016: Heliyon
https://www.readbyqxmd.com/read/28000914/inclusion-of-hermetia-illucens-larvae-meal-on-rainbow-trout-oncorhynchus-mykiss-feed-effect-on-sensory-profile-according-to-static-and-dynamic-evaluations
#4
Monica Borgogno, Caterina Dinnella, Valeria Iaconisi, Renzo Fusi, Claudia Scarpaleggia, Achille Schiavone, Erminio Monteleone, Laura Gasco, Giuliana Parisi
BACKGROUND: Diet implementation with insect meal arouses increased attention in aquaculture considering the advantages of this new protein source. The effect of Hermetia illucens meal (HI) inclusion in diets on rainbow trout physical-chemical and sensory properties was evaluated. Three diets were prepared: HI0, HI25, HI50, with 0, 25 and 50% of HI replacing fish meal, respectively. Fillet sensory profiles were described by descriptive analysis (DA) and Temporal Dominance of Sensation (TDS) methods...
December 21, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27988568/description-of-the-puparium-of-i-hermetia-i-i-pulchra-i-diptera-stratiomyidae-%C3%A2-from-brazil
#5
José Roberto Pujol-Luz, Fabio Siqueira Pitaluga DE Godoi, Karine Brenda Barros-Cordeiro
The puparium of Hermetia pulchra is described, based on a single specimen reared in laboratory under controlled conditions until the emergence of an adult female. The puparium of H. pulchra was compared with puparium of H. illucens and some differential features were indicated. A checklist of the Brazilian species of the genus Hermetia is included, with new distributional records for H. pulchra in the Brazilian States of Bahia and Distrito Federal. Hermetia sphecodes is recorded for the first time for Brazil (State of Amazonas)...
December 7, 2016: Zootaxa
https://www.readbyqxmd.com/read/27986944/survival-and-development-of-hermetia-illucens-diptera-stratiomyidae-a-biodegradation-agent-of-organic-waste
#6
Ana Clariza Samayoa, Wei-Ting Chen, Shaw-Yhi Hwang
: The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), was reared on artificial diet (wheat bran and chicken feed) in the laboratory at 28ºC (immature stages) and under a greenhouse set at 28ºC (adults). Data were collected and analyzed based on an age-stage, two-sex life table. The intrinsic rate of increase (r), finite rate of increase (λ), net reproduction rate (R0), and mean generation time (T) were 0.0759 (d(-1)), 1.0759 (d(-1)), 68.225 offspring, and 55.635 d, respectively...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27903420/microbiology-of-processed-edible-insect-products-results-of-a-preliminary-survey
#7
Nils Th Grabowski, Günter Klein
Little is known of the microbiology of processed insect products. The present survey analysed a total of n=38 samples of deep-fried and spiced (Acheta domesticus, Locusta migratoria, and Omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; Oxya yezoensis, Vespula flaviceps, and Bombyx mori), dried (A. domesticus, L. migatoria, Alphitobius diaperinus, Tenebrio molitor, B. mori, Hermetia illucens, and Musca domestica), powdered (H. illucens, T. molitor) and other (incl. deep-frozen B. mori and honeybee pollen) insect products microbiologically (total bacterial count [TBC], Enterobacteriaceae, staphylococci, bacilli, and yeasts and moulds counts, salmonellae, Listeria monocytogenes, and Escherichia coli)...
November 9, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27846238/uptake-of-cadmium-lead-and-arsenic-by-tenebrio-molitor-and-hermetia-illucens-from-contaminated-substrates
#8
H J van der Fels-Klerx, L Camenzuli, M K van der Lee, D G A B Oonincx
Insects have potential as a novel source of protein in feed and food production in Europe, provided they can be used safely. To date, limited information is available on the safety of insects, and toxic elements are one of the potential hazards of concern. Therefore, we aimed to investigate the potential accumulation of cadmium, lead and arsenic in larvae of two insect species, Tenebrio molitor (yellow mealworm) and Hermetia illucens (black soldier fly), which seem to hold potential as a source of food or feed...
2016: PloS One
https://www.readbyqxmd.com/read/27751886/photoreceptor-spectral-sensitivity-of-the-compound-eyes-of-black-soldier-fly-hermetia-illucens-informing-the-design-of-led-based-illumination-to-enhance-indoor-reproduction
#9
D G A B Oonincx, N Volk, J J E Diehl, J J A van Loon, G Belušič
Mating in the black soldier fly (BSF) is a visually mediated behaviour that under natural conditions occurs in full sunlight. Artificial light conditions promoting mating by BSF were designed based on the spectral characteristics of the compound eye retina. Electrophysiological measurements revealed that BSF ommatidia contained UV-, blue- and green-sensitive photoreceptor cells, allowing trichromatic vision. An illumination system for indoor breeding based on UV, blue and green LEDs was designed and its efficiency was compared with illumination by fluorescent tubes which have been successfully used to sustain a BSF colony for five years...
December 2016: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27734508/nutritional-composition-of-black-soldier-fly-hermetia-illucens-prepupae-reared-on-different-organic-waste-substrates
#10
Thomas Spranghers, Matteo Ottoboni, Cindy Klootwijk, Anneke Ovyn, Stefaan Deboosere, Bruno De Meulenaer, Joris Michiels, Mia Eeckhout, Patrick De Clercq, Stefaan De Smet
BACKGROUND: Black soldier fly larvae are converters of organic waste into edible biomass, of which the composition may depend on the substrate. In this study, larvae were grown on four substrates: chicken feed, vegetable waste, biogas digestate, and restaurant waste. Samples of prepupae and substrates were freeze-dried and proximate, amino acid, fatty acid and mineral analyses were performed. RESULTS: Protein content of prepupae varied between 399 and 431 g kg(-1) dry matter (DM) among treatments...
October 13, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27616765/survival-and-development-of-hermetia-illucens-diptera-stratiomyidae-a-biodegradation-agent-of-organic-waste
#11
Ana Clariza Samayoa, Wei-Ting Chen, Shaw-Yhi Hwang
The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), was reared on artificial diet (wheat bran and chicken feed) in the laboratory at 28°C (immature stages) and under a greenhouse set at 28°C (adults). Data were collected and analyzed based on an age-stage, two-sex life table. The intrinsic rate of increase (r), finite rate of increase (λ), net reproduction rate (R0), and mean generation time (T) were 0.0759 (d(-1)), 1.0759 (d(-1)), 68.225 offspring, and 55.635 d, respectively. The maximum reproductive value of females occurred at 54 d...
September 11, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27422042/the-first-report-of-the-physicochemical-structure-of-chitin-isolated-from-hermetia-illucens
#12
Adam Waśko, Piotr Bulak, Magdalena Polak-Berecka, Katarzyna Nowak, Cezary Polakowski, Andrzej Bieganowski
This is the first report on the physicochemical properties of chitin obtained from larvae and imagoes of black soldier flies (Hermetia illucens). Scanning electron microscopy revealed differences in surface morphologies of the two types of chitin. The crystalline index values of chitins from adult flies and larvae were 24.9% and 35%, respectively. This is a trait that differentiates these biopolymers from chitins extracted from other sources described so far. X-ray diffraction patterns and IR spectroscopy revealed that both types of samples of chitin were in an α crystalline form...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27417723/characteristics-of-lipid-fractions-of-larvae-of-the-black-soldier-fly-hermetia-illucens
#13
N A Ushakova, E S Brodskii, A A Kovalenko, A I Bastrakov, A A Kozlova, D S Pavlov
The lipid fraction of larvae of the black soldier fly Hermetia illucens was shown to contain lauric acid (38.43 wt %) and its esters, azelaic and sebacic acids, and azelaic acid dibutyl ester. The dominant compound in the group of identified glycerides was lauric acid monoglyceride (0.70 wt %). Glycerides were also represented by triglycerides and diglycerides of lauric acid. Sterols were represented primarily by phytosterols (over 75%), the major of which was alpha-sitosterol (45%). The identified lipid complex composition is apparently determined by the biological characteristics of the fly Hermetia illucens and ensures antibacterial defence of larvae and stability of lipids at changing ambient temperature...
May 2016: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27339654/black-soldier-fly-as-dietary-protein-source-for-broiler-quails-apparent-digestibility-excreta-microbial-load-feed-choice-performance-carcass-and-meat-traits
#14
M Cullere, G Tasoniero, V Giaccone, R Miotti-Scapin, E Claeys, S De Smet, A Dalle Zotte
In order to expand with validated scientific data the limited knowledge regarding the potential application of insects as innovative feed ingredients for poultry, the present study tested a partial substitution of soya bean meal and soya bean oil with defatted black soldier fly (Hermetia illucens) larvae meal (H) in the diet for growing broiler quails (Coturnix coturnix japonica) on growth performance, mortality, nutrients apparent digestibility, microbiological composition of excreta, feed choice, carcass and meat traits...
December 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27236368/effects-of-temperature-and-diet-on-black-soldier-fly-hermetia-illucens-l-diptera-stratiomyidae-development
#15
Laura M Harnden, Jeffery K Tomberlin
The black soldier fly, Hermetia illucens, is recognised for its use in a forensic context as a means for estimating the time of colonisation and potentially postmortem interval of decomposing remains. However, little data exist on this species outside of its use in waste management. This study offers a preliminary assessment of the development, and subsequent validation, of H. illucens. Larvae of H. illucens were reared at three temperatures (24.9°C, 27.6°C and 32.2°C) at 55% RH on beef loin muscle, pork loin muscle and a grain-based diet (control)...
September 2016: Forensic Science International
https://www.readbyqxmd.com/read/26921223/supplementation-of-an-artificial-medium-for-the-parasitoid-exorista-larvarum-diptera-tachnidae-with-hemolymph-of-hermetia-illucens-diptera-stratiomyidae-or-antheraea-pernyi-lepidoptera-saturniidae
#16
Maria Luisa Dindo, Jonas Vandicke, Elisa Marchetti, Thomas Spranghers, Jochem Bonte, Patrick De Clercq
The effect of supplementing hemolymph of the black soldier fly, Hermetia illucens (L.), or the Chinese oak silkworm, Antheraea pernyi (Guérin-Méneville), to a basic insect-free artificial medium for the tachinid Exorista larvarum (L.) was investigated. The supplementation (20% w/w) was based on the assumption that insect additives may optimize the media for this parasitoid. Egg hatch, pupal and adult yields, and sex ratio did not differ among the enriched and basic media. Preimaginal development was faster on both hemolymph-enriched media than on the basic medium...
April 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/26526170/identification-of-a-novel-alkaline-amylopullulanase-from-a-gut-metagenome-of-hermetia-illucens
#17
Young-Seok Lee, So-Hyeon Seo, Sang-Hong Yoon, Su-Yeon Kim, Bum-Soo Hahn, Joon-Soo Sim, Bon-Sung Koo, Chang-Muk Lee
A novel pullulanase gene, PulSS4, was identified from the gut microflora of Hermetia illucens by a function-based metagenome screening. The PulSS4 gene had an open reading frame of 4455 base pairs, and encoded a mature protein of 1484 amino acids, with a signal peptide sequence of 44 amino acids. The deduced amino acid sequence of PulSS4 gene showed 51% identity with that of the amylopullulanase of Amphibacillus xylanus, exhibiting no significant sequence homology to already known pullulanases. A conserved domain analysis revealed it to be a pullulanase type II with respective active sites at the N-terminal pullulanase and C-terminal amylase domain...
January 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26313195/ability-of-black-soldier-fly-diptera-stratiomyidae-larvae-to-recycle-food-waste
#18
Trinh T X Nguyen, Jeffery K Tomberlin, Sherah Vanlaerhoven
Accumulation of organic wastes, especially in livestock facilities, can be a potential pollution issue. The black soldier fly, Hermetia illucens L. (Diptera: Stratiomyidae), can consume a wide range of organic material and has the potential to be used in waste management. In addition, the prepupae stage of this insect can be harvested and used as a valuable nutritious feed for animal livestock. Five waste types with a wide range of organic source matter were specifically chosen to evaluate the consumption and reduction ability of black soldier fly larvae...
April 2015: Environmental Entomology
https://www.readbyqxmd.com/read/25956195/purification-and-characterization-of-a-novel-antibacterial-peptide-from-black-soldier-fly-hermetia-illucens-larvae
#19
Soon-Ik Park, Jong-Wan Kim, Sung Moon Yoe
In this study, we induced and purified a novel antimicrobial peptide exhibiting activity against Gram-positive bacteria from the immunized hemolymph of Hermetia illucens larvae. The immunized hemolymph was extracted, and the novel defensin-like peptide 4 (DLP4) was purified using solid-phase extraction and reverse-phase chromatography. The purified DLP4 demonstrated a molecular weight of 4267 Da, as determined using the matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) method. From analysis of DLP4 by N-terminal amino acid sequencing using Edman degradation, combined with MALDI-TOF and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR), the amino acid sequence of the mature peptide was determined to be ATCDLLSPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK...
September 2015: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/25872864/feasibility-study-of-biodiesel-production-using-lipids-of-hermetia-illucens-larva-fed-with-organic-waste
#20
Siew Yoong Leong, Shamsul Rahman Mohamed Kutty, Amirhossein Malakahmad, Chew Khun Tan
Hermetia illucens larvae by nature are a decomposer which fed on organic wastes. This study explores the potential of producing biodiesel using lipids from H. illucens larvae. Three types of organic wastes (sewage sludge, fruit waste and palm decanter cake from oil palm mill) were selected based on considerable generation and disposal concern in the area of study as well as lack of investigations as feed for Hermetia illucens larvae in current literatures. Growth rate of the larvae was determined with studying the changes in the biomass per day...
January 2016: Waste Management
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