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Fuchs cornea

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https://www.readbyqxmd.com/read/28630424/characterization-of-slow-cycling-corneal-limbal-epithelial-cells-identifies-putative-stem-cell-markers
#1
R Sartaj, C Zhang, P Wan, Z Pasha, V Guaiquil, A Liu, J Liu, Y Luo, E Fuchs, M I Rosenblatt
In order to identify reliable markers of corneal epithelial stem cells, we employed an inducible transgenic "pulse-chase" murine model (K5Tta × TRE-H2BGFP) to localize, purify, and characterize slow cycling cells in the cornea. The retention of GFP labeling in slowly dividing cells allowed for localization of these cells to the corneal limbus and their subsequent purification by FACS. Transcriptome analysis from slow cycling cells identified differentially expressed genes when comparing to GFP(-) faster-dividing cells...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608272/ctg18-1-repeat-expansion-may-reduce-tcf4-gene-expression-in-corneal-endothelial-cells-of-german-patients-with-fuchs-dystrophy
#2
Sabine Foja, Mirjam Luther, Katrin Hoffmann, Andreas Rupprecht, Claudia Gruenauer-Kloevekorn
PURPOSE: It was the aim of this investigation to elucidate the functional effects of CTG18.1 trinucleotide repeat expansion and the polymorphism rs613872 in the transcription factor 4 (TCF4) in corneas of patients affected by Fuchs' endothelial corneal dystrophy (FECD). METHODS: Sixty-one unrelated German patients with FECD and 113 unaffected controls were investigated and genotyped for the CTG18.1 locus by triplet primed PCR (TP-PCR) and the rs613872 polymorphism via Sanger sequencing and by employing genomic DNA from peripheral blood leucocytes...
June 12, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28549842/mitochondrial-dysfunction-and-oxidative-stress-in-corneal-disease
#3
Neeru A Vallabh, Vito Romano, Colin E Willoughby
The cornea is the anterior transparent surface and the main refracting structure of the eye. Mitochondrial dysfunction and oxidative stress are implicated in the pathogenesis of inherited (e.g. Kearns Sayre Syndrome) and acquired corneal diseases (e.g. keratoconus and Fuchs endothelial corneal dystrophy). Both antioxidants and reactive oxygen species are found in the healthy cornea. There is increasing evidence of imbalance in the oxidative balance and mitochondrial function in the cornea in disease states...
May 23, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28489792/eye-bank-prepared-versus-surgeon-cut-endothelial-graft-tissue-for-descemet-membrane-endothelial-keratoplasty-an-observational-study
#4
COMPARATIVE STUDY
Marie Regnier, Céline Auxenfans, Delphine Maucort-Boulch, Anne-Sophie Marty, Odile Damour, Carole Burillon, Viridiana Kocaba
The purpose of this article is to examine outcomes of Descemet membrane endothelial keratoplasty (DMEK) performed with cornea bank (CB) prestripped tissue and surgeon stripped tissue (SST).This retrospective study examined subjects who underwent DMEK with CB or surgeon prepared tissue for Fuchs endothelial corneal dystrophy. Best-corrected visual acuity (BCVA), corneal thickness, endothelial cell count (ECC), and complications were examined before and throughout a 6-month postoperative period.Eleven CB and 22 SST subjects were included...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28486726/management-of-interface-fluid-syndrome-after-lasik-by-descemet-membrane-endothelial-keratoplasty-in-a-patient-with-fuchs-corneal-endothelial-dystrophy
#5
Mehdi Shajari, Parviz Rafiezadeh, Ivana Pavlovic, Kate Barbara Kubiak, Thomas Kohnen, Ingo Schmack
PURPOSE: To report a case of interface fluid syndrome after LASIK in a patient with Fuchs' corneal endothelial dystrophy treated with Descemet membrane endothelial keratoplasty (DMEK). METHODS: Case report. RESULTS: A 74-year-old patient presented with interface fluid syndrome and was treated with DMEK. Its complications in the form of partial graft detachment and cystoid macular edema were successfully managed by rebubbling and topical treatment, respectively...
May 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28481834/immunohistochemical-profiling-of-corneas-with-fuchs-endothelial-corneal-dystrophy
#6
An-Katrien De Roo, Thomas Janssens, Beatrijs Foets, Joost J van den Oord
PURPOSE: Fuchs endothelial corneal dystrophy (FECD) is the leading indication for endothelial keratoplasty. Further insight into its pathophysiology is needed to develop alternative therapies. METHODS: Sixteen genes from a previous microarray expression experiment (FECD vs. normal) were validated using immunohistochemistry on paraffin-embedded corneas (n = 6 FECD, n = 6 normal). The results were quantified manually and semiautomatically. RESULTS: A higher percentage of corneal endothelial cells stained for alpha-smooth muscle actin (αSMA), cytokeratin 7, and superoxide dismutase 3 in FECD versus normal [odds ratios (ORs) of 60...
July 2017: Cornea
https://www.readbyqxmd.com/read/28384203/comprehensive-characterization-of-dna-methylation-changes-in-fuchs-endothelial-corneal-dystrophy
#7
Emily Khuc, Russell Bainer, Marie Wolf, Selene M Clay, Daniel J Weisenberger, Jacquelyn Kemmer, Valerie M Weaver, David G Hwang, Matilda F Chan
Transparency of the human cornea is necessary for vision. Fuchs Endothelial Corneal Dystrophy (FECD) is a bilateral, heritable degeneration of the corneal endothelium, and a leading indication for corneal transplantation in developed countries. While the early onset, and rarer, form of FECD has been linked to COL8A2 mutations, the more common, late onset form of FECD has genetic mutations linked to only a minority of cases. Epigenetic modifications that occur in FECD are unknown. Here, we report on and compare the DNA methylation landscape of normal human corneal endothelial (CE) tissue and CE from FECD patients using the Illumina Infinium HumanMethylation450 (HM450) DNA methylation array...
2017: PloS One
https://www.readbyqxmd.com/read/28381247/outcomes-of-descemet-stripping-automated-endothelial-keratoplasty-using-imported-donor-corneas
#8
Kaevalin Lekhanont, Kavin Vanikieti, Nutthida Nimvorapun, Varintorn Chuckpaiwong
BACKGROUND: The lack of development of local donor tissue acquisition in several regions of the world has resulted in the necessity of performing keratoplasty with imported donor corneas. The greatest concern about the use of donor corneas supplied by foreign eye banks is the effect of the increased donor death-to-operation time which inevitably occurs during the tissue recovery, tissue processing, and tissue transfer between the countries. The purpose of this study was to report the outcomes of descemet stripping automated endothelial keratoplasty (DSAEK) using imported donor corneas...
April 5, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28358029/genome-wide-association-study-identifies-three-novel-loci-in-fuchs-endothelial-corneal-dystrophy
#9
Natalie A Afshari, Robert P Igo, Nathan J Morris, Dwight Stambolian, Shiwani Sharma, V Lakshmi Pulagam, Steven Dunn, John F Stamler, Barbara J Truitt, Jacqueline Rimmler, Abraham Kuot, Christopher R Croasdale, Xuejun Qin, Kathryn P Burdon, S Amer Riazuddin, Richard Mills, Sonja Klebe, Mollie A Minear, Jiagang Zhao, Elmer Balajonda, George O Rosenwasser, Keith H Baratz, V Vinod Mootha, Sanjay V Patel, Simon G Gregory, Joan E Bailey-Wilson, Marianne O Price, Francis W Price, Jamie E Craig, John H Fingert, John D Gottsch, Anthony J Aldave, Gordon K Klintworth, Jonathan H Lass, Yi-Ju Li, Sudha K Iyengar
The structure of the cornea is vital to its transparency, and dystrophies that disrupt corneal organization are highly heritable. To understand the genetic aetiology of Fuchs endothelial corneal dystrophy (FECD), the most prevalent corneal disorder requiring transplantation, we conducted a genome-wide association study (GWAS) on 1,404 FECD cases and 2,564 controls of European ancestry, followed by replication and meta-analysis, for a total of 2,075 cases and 3,342 controls. We identify three novel loci meeting genome-wide significance (P<5 × 10(-8)): KANK4 rs79742895, LAMC1 rs3768617 and LINC00970/ATP1B1 rs1200114...
March 30, 2017: Nature Communications
https://www.readbyqxmd.com/read/28346276/comparison-of-noncontact-specular-and-confocal-microscopy-for-evaluation-of-corneal-endothelium
#10
Jianyan Huang, Jyotsna Maram, Tudor C Tepelus, Srinivas R Sadda, Vikas Chopra, Olivia L Lee
PURPOSE: To compare endothelial cell analysis obtained by noncontact specular and confocal microscopy, using the Konan NSP-9900 and Nidek ConfoScan4 systems, respectively. METHODS: Three groups including 70 healthy eyes, 49 eyes with Fuchs endothelial corneal dystrophy (FECD), and 78 eyes with glaucoma were examined with both the Konan NSP-9900 specular microscope and the Nidek ConfocScan4 confocal microscope. Certified graders at the Doheny Image Reading Center compared corneal endothelial images from both instruments side by side to assess image quality...
March 24, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28271013/ultrahigh-resolution-oct-imaging-of-the-human-cornea
#11
René M Werkmeister, Sabina Sapeta, Doreen Schmidl, Gerhard Garhöfer, Gerald Schmidinger, Valentin Aranha Dos Santos, Gerold C Aschinger, Isabella Baumgartner, Niklas Pircher, Florian Schwarzhans, Anca Pantalon, Harminder Dua, Leopold Schmetterer
We present imaging of corneal pathologies using optical coherence tomography (OCT) with high resolution. To this end, an ultrahigh-resolution spectral domain OCT (UHR-OCT) system based on a broad bandwidth Ti:sapphire laser is employed. With a central wavelength of 800 nm, the imaging device allows to acquire OCT data at the central, paracentral and peripheral cornea as well as the limbal region with 1.2 µm x 20 µm (axial x lateral) resolution at a rate of 140 000 A-scans/s. Structures of the anterior segment of the eye, not accessible with commercial OCT systems, are visualized...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28060074/eventual-endothelial-failure-after-initial-corneal-clearing-after-a-detached-endothelial-graft-in-fuchs-dystrophy
#12
Hyunjoo J Lee, Wuqaas M Munir
PURPOSE: To report a case of eventual corneal endothelial cell failure after spontaneous resolution of corneal edema after failed Descemet membrane endothelial keratoplasty (DMEK). METHODS: Retrospective case report. RESULTS: A 56-year-old man with Fuchs endothelial corneal dystrophy underwent cataract and DMEK surgery in the left eye. The transplanted DMEK graft detached in the early postoperative period and formed a peripheral scroll. Despite graft detachment, corneal edema resolved with endothelial cell repopulation of the central cornea...
February 2017: Cornea
https://www.readbyqxmd.com/read/27974291/organ-cultured-prestripped-donor-tissue-for-dmek-surgery-clinical-outcomes
#13
Johannes Menzel-Severing, Friedrich E Kruse, Theofilos Tourtas
AIM: To determine whether clinical performance is negatively affected by prestripping Descemet membrane endothelial keratoplasty (DMEK) grafts from organ-cultured corneas. METHODS: We reviewed clinical records of all patients who underwent DMEK surgery for Fuchs endothelial dystrophy between 28 October 2014 and 11 August 2015. Grafts had been prepared from organ-cultured corneoscleral buttons 24 hours prior to surgery or during surgery. We included only patients for which at least one follow-up examination was available at a minimum of 2 months postoperatively...
December 14, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27965261/very-early-endothelial-cell-loss-after-penetrating-keratoplasty-with-organ-cultured-corneas
#14
Anne Sophie Gauthier, Thibaud Garcin, Gilles Thuret, Zhiguo He, Remy Jullienne, Marie Caroline Trone, Chaker Nefzaoui, Sophie Acquart, Fabien Forest, Michel Péoc'h, Bernard Delbosc, Philippe Gain
AIMS: After keratoplasty, postoperative endothelial cell loss is calculated between the eye bank endothelial cell density (ebECD) and the postoperative specular microscopy (SM). To elucidate the very early cell loss, always described after penetrating keratoplasty (PK), we designed two complementary studies. METHODS: (1) Clinical prospective study of 90 consecutive PKs (keratoconus, Fuchs' corneal dystrophy, lattice dystrophy, bullous keratopathy) with organ-cultured corneas and postoperative follow-up by SM at day 5 (D5), D15, month 1 (M1) and M3...
December 13, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27941386/ten-year-clinical-outcome-of-the-first-patient-undergoing-descemet-membrane-endothelial-keratoplasty
#15
Lamis Baydoun, Thomas Müller, Itay Lavy, Jack Parker, Marina Rodriguez-Calvo-de-Mora, Vasilios S Liarakos, Isabel Dapena, Gerrit R J Melles
PURPOSE: To describe the 10-year clinical outcome of the first patient worldwide who underwent Descemet membrane endothelial keratoplasty (DMEK). METHODS: In 2006, a 63-year-old man presented at the Melles Cornea Clinic, Rotterdam, with bilateral Fuchs endothelial dystrophy and cataract. After phacoemulsification, in vivo DMEK was performed in the left eye and 10 months later in the right eye. Best spectacle-corrected visual acuity (BSCVA), endothelial cell density, pachymetry, and complications were recorded every 6 months over a 10-year period...
March 2017: Cornea
https://www.readbyqxmd.com/read/27811565/automated-retroillumination-photography-analysis-for-objective-assessment-of-fuchs-corneal-dystrophy
#16
Allen O Eghrari, Aisha A Mumtaz, Brian Garrett, Mahsa Rezaei, Mina S Akhavan, S Amer Riazuddin, John D Gottsch
PURPOSE: Retroillumination photography analysis is an objective tool for the assessment of the number and distribution of guttae in eyes affected with Fuchs corneal dystrophy (FCD). Current protocols include manual processing of images; here, we assess validity and interrater reliability of automated analysis across various levels of FCD severity. METHODS: Retroillumination photographs of 97 FCD-affected corneas were acquired, and total counts of guttae were previously summated manually...
January 2017: Cornea
https://www.readbyqxmd.com/read/27760648/-study-on-the-evaluation-of-pterygium-activity-with-in-vivo-confocal-microscopy
#17
Q F Liang, C Gao, H Liang, B Li, X H Du, Antoine Labbe
Objective: To observe the characteristics of pterygium with in vivo confocal microscopy (IVCM) and understand pterygium activity with the density of inflammatory cells, formation of new blood vessels, and the number of activated keratocytes within the stroma. Methods: A prospective case-controlled study. Thirty-six pterygia from 28 patients were analyzed in this study. A pterygium activity score was obtained by summing four scores of ocular discomfort, pterygium hyperemia, keratitis, and the presence of Fuchs patches...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27665214/histopathologic-features-of-descemet-membrane-endothelial-keratoplasty-graft-remnants-folds-and-detachments
#18
Thomas M Müller, Robert M Verdijk, Itay Lavy, Marieke Bruinsma, Jack Parker, Perry S Binder, Gerrit R J Melles
PURPOSE: To describe the histologic features of postmortem eyes after Descemet membrane endothelial keratoplasty (DMEK) and their potential clinical implications. DESIGN: Histopathologic study. PARTICIPANTS: Eleven postmortem DMEK corneas of 8 patients who underwent surgery for Fuchs endothelial dystrophy, with an average postoperative time of 4±1.9 years (range, 7 months-6.5 years). METHODS: Eleven corneas transplanted with a DMEK graft were procured after death and processed for light microscopy evaluation...
December 2016: Ophthalmology
https://www.readbyqxmd.com/read/27661858/corneal-hydration-control-in-fuchs-endothelial-corneal-dystrophy
#19
Katrin Wacker, Jay W McLaren, Katrina M Kane, Keith H Baratz, Sanjay V Patel
Purpose: To assess corneal hydration control across a range of severity of Fuchs' endothelial corneal dystrophy (FECD) by measuring the percent recovery per hour (PRPH) of central corneal thickness after swelling the cornea and to determine its association with corneal morphologic parameters. Methods: Twenty-three corneas of 23 phakic FECD patients and 8 corneas of 8 healthy control participants devoid of guttae were graded (modified Krachmer scale). Effective endothelial cell density (ECDe) was determined from the area of guttae and local cell density in confocal microscopy images...
September 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27661069/two-year-refractive-outcomes-after-descemet-membrane-endothelial-keratoplasty
#20
Korine van Dijk, Marina Rodriguez-Calvo-de-Mora, Hilde van Esch, Laurence Frank, Isabel Dapena, Lamis Baydoun, Silke Oellerich, Gerrit R J Melles
PURPOSE: To monitor refractive changes after Descemet membrane endothelial keratoplasty (DMEK) and to determine what may influence these changes and the time point of stabilization. METHODS: From 67 pseudophakic DMEK eyes operated on for Fuchs endothelial dystrophy at a tertiary referral center, biomicroscopy, visual acuity, subjective refraction, and Scheimpflug-based corneal tomography data were obtained before and up to 2 years postoperatively. Visual acuity and changes in spherical equivalent (SE), mean anterior and posterior simulated keratometry (Km), and central pachymetry were analyzed...
December 2016: Cornea
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