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https://www.readbyqxmd.com/read/28209236/epithelial-downgrowth-report-of-2-cases-diagnosed-by-ocular-biopsy
#1
REVIEW
Hershel R Patel, Curtis E Margo
Epithelial downgrowth is an uncommon complication of ocular surgery or adverse consequence of accidental trauma, caused by surface squamous epithelium that has gained access to the inner compartments of the eye. Once embedded in the eye, squamous epithelium spreads over contiguous structures, interfering with normal aqueous outflow and vision. The pursuit of improved therapies is ongoing, but the greatest chance of preventing blindness is with early recognition. Two cases of epithelial downgrowth diagnosed from a corneal button and an iris biopsy are presented to familiarize pathologists with this disorder...
February 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28181929/corneal-endothelial-cell-integrity-in-precut-human-donor-corneas-enhanced-by-autocrine-vasoactive-intestinal-peptide
#2
Shay-Whey M Koh, Timothy Coll, Dante Gloria, Nicholas Sprehe
PURPOSE: To demonstrate that vasoactive intestinal peptide (VIP), a corneal endothelial (CE) cell autocrine factor, maintains the integrity of corneal endothelium in human donor corneoscleral explants precut for endothelial keratoplasty. METHODS: Twelve paired human donor corneoscleral explants used as control versus VIP-treated explants (10 nM, 30 minutes, 37°C) were shipped (4°C) to the Lions Eye Institute for Transplantation and Research for precutting (Moria CBM-ALTK Keratome), shipped back to the laboratory, and cultured in ciliary neurotrophic factor (CNTF, 0...
February 8, 2017: Cornea
https://www.readbyqxmd.com/read/28158775/therapeutic-potential-of-aav-mediated-mmp-3-secretion-from-corneal-endothelium-in-treating-glaucoma
#3
Jeffrey O'Callaghan, Darragh E Crosbie, Paul S Cassidy, Joseph M Sherwood, Cassandra Flügel-Koch, Elke Lütjen-Drecoll, Marian M Humphries, Ester Reina-Torres, Deborah Wallace, Anna-Sophia Kiang, Matthew Campbell, Stamer W Daniel, Darryl R Overby, Colm O'Brien, Lawrence C S Tam, Peter Humphries
No abstract text is available yet for this article.
February 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28151811/effect-of-trabeculotomy-on-corneal-endothelial-cell-loss-in-cases-of-after-penetrating-keratoplasty-glaucoma
#4
Ayaka Kusakabe, Naoki Okumura, Koichi Wakimasu, Kanae Kayukawa, Masami Kondo, Noriko Koizumi, Chie Sotozono, Shigeru Kinoshita, Kazuhiko Mori
PURPOSE: The aim of this study was to evaluate the effect of trabeculotomy (TLO) on glaucoma and endothelial cell loss after penetrating keratoplasty (PK). METHODS: A retrospective study was conducted on consecutive patients who underwent PK and in whom more than 24 months of follow-up was available. Patients were categorized into the PK+TLO group [ie, TLO for post-PK glaucoma (n = 10)] and the PK group [PK alone (n = 73)]. Intraocular pressure (IOP) was evaluated during each follow-up examination...
March 2017: Cornea
https://www.readbyqxmd.com/read/28146239/combined-pi3k-akt-and-smad2-activation-promotes-corneal-endothelial-cell-proliferation
#5
Alfonso L Sabater, Enrique J Andreu, María García-Guzmán, Tania López, Gloria Abizanda, Victor L Perez, Javier Moreno-Montañés, Felipe Prósper
Purpose: The purpose of this study was to develop a culture method for expansion of corneal endothelial cells (CEC) based on the combined activation of PI3K/Akt and Smad2. Methods: Morphology, proliferation, and migration of cultured rabbit and nonhuman primate CEC were examined in the presence of the PI3K/Akt activators IGF-1 and heregulin beta in combination with the Smad2 activator activin A. Phenotypic characterization of CEC was performed at the RNA and protein levels...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28135362/identification-of-circulating-fibrocytes-and-dendritic-derivatives-in-corneal-endothelium-of-patients-with-fuchs-dystrophy
#6
An-Katrien De Roo, Jasper Wouters, Olivier Govaere, Beatrijs Foets, Joost J van den Oord
Purpose: Fuchs' endothelial corneal dystrophy (FECD) is a degenerative eye disorder affecting 4% of Americans older than 40. It is the leading indication for corneal endothelial (CE) transplantation for which there is a global donor shortage. This study aimed to gain further insight into the pathophysiology of FECD and identify targets for nonsurgical therapy. Methods: CE from patients with late-onset FECD was compared with that of normal controls using microarray expression analysis (n = 4 FECD, n = 4 normal), reverse transcriptase quantitative PCR (n = 9 FECD, n = 8 normal), and immunohistology (n = 55 FECD, n = 15 normal)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28128337/cornea-organoids-from-human-induced-pluripotent-stem-cells
#7
James W Foster, Karl Wahlin, Sheila M Adams, David E Birk, Donald J Zack, Shukti Chakravarti
The cornea is the transparent outermost surface of the eye, consisting of a stratified epithelium, a collagenous stroma and an innermost single-cell layered endothelium and providing 2/3 of the refractive power of the eye. Multiple diseases of the cornea arise from genetic defects where the ultimate phenotype can be influenced by cross talk between the cell types and the extracellular matrix. Cell culture modeling of diseases can benefit from cornea organoids that include multiple corneal cell types and extracellular matrices...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28118661/trinucleotide-repeat-expansion-in-the-transcription-factor-4-tcf4-gene-leads-to-widespread-mrna-splicing-changes-in-fuchs-endothelial-corneal-dystrophy
#8
Eric D Wieben, Ross A Aleff, Xiaojia Tang, Malinda L Butz, Krishna R Kalari, Edward W Highsmith, Jin Jen, George Vasmatzis, Sanjay V Patel, Leo J Maguire, Keith H Baratz, Michael P Fautsch
Purpose: To identify RNA missplicing events in human corneal endothelial tissue isolated from Fuchs' endothelial corneal dystrophy (FECD). Methods: Total RNA was isolated and sequenced from corneal endothelial tissue obtained during keratoplasty from 12 patients with FECD and 4 patients undergoing keratoplasty or enucleation for other indications. The length of the trinucleotide repeat (TNR) CTG in the transcription factor 4 (TCF4) gene was determined using leukocyte-derived DNA analyzed by a combination of Southern blotting and Genescan analysis...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28117276/glutaminolysis-is-essential-for-energy-production-and-ion-transport-in-human-corneal-endothelium
#9
Wenlin Zhang, Hongde Li, Diego G Ogando, Shimin Li, Matthew Feng, Francis W Price, Jason M Tennessen, Joseph A Bonanno
Corneal endothelium (CE) is among the most metabolically active tissues in the body. This elevated metabolic rate helps the CE maintain corneal transparency by its ion and fluid transport properties, which when disrupted, leads to visual impairment. Here we demonstrate that glutamine catabolism (glutaminolysis) through TCA cycle generates a large fraction of the ATP needed to maintain CE function, and this glutaminolysis is severely disrupted in cells deficient in NH3:H(+) cotransporter Solute Carrier Family 4 Member 11 (SLC4A11)...
January 13, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28114585/immune-cells-on-the-corneal-endothelium-of-an-allogeneic-corneal-transplantation-rabbit-model
#10
Elena Koudouna, Naoki Okumura, Yugo Okazaki, Shinichiro Nakano, Ryota Inoue, Nigel J Fullwood, Junko Hori, Shigeru Kinoshita, Noriko Koizumi
Purpose: Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. Methods: The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet's stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28101654/cell-viability-and-shock-wave-amplitudes-in-the-endothelium-of-porcine-cornea-exposed-to-ultrashort-laser-pulses
#11
Syed Asad Hussain, Carles Milián, Caroline Crotti, Laura Kowalczuk, Fatima Alahyane, Zacaria Essaïdi, Arnaud Couairon, Marie-Claire Schanne-Klein, Karsten Plamann
PURPOSE: Some forms of keratoplasty assisted by ultrashort-pulse lasers require performing laser cuts close to the endothelium, which requires the knowledge of "safe" values concerning incision depth and pulse energy preserving endothelial cell viability. Our study aims to determine the thresholds for cell death in porcine corneas exposed to ultrashort laser pulses, in terms of laser pulse energy and nearness of the impacts to the endothelium. METHODS: Using a laboratory laser set-up, lamellar cuts were induced while varying pulse energies and distances from the endothelium...
January 18, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28100180/extensive-bilateral-corneal-edema-6%C3%A2-weeks-after-cataract-surgery-keratopathy-due-to-asclepias-physocarpa-a-case-report
#12
Kazuki Matsuura, Shiro Hatta, Yuki Terasaka, Yoshitsugu Inoue
BACKGROUND: Surgeons may be unaware of the ability of plant toxins to cause corneal damage. Therefore, corneal damage following intraocular surgery due to plant toxins may be misdiagnosed as postoperative infection. CASE PRESENTATION: A 74-year-old man presented with hyperemia and reduced visual acuity in both eyes 6 weeks after uneventful cataract surgery. We observed extensive hyperemia and corneal stromal edema with Descemet's folds in both eyes. After obtaining a detailed patient history, we diagnosed plant toxin-induced corneal edema due to Asclepias physocarpa, which can induce corneal edema by inhibiting the Na(+)/K(+) ATPase activity of the corneal endothelium...
January 18, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28079691/lutein-and-brilliant-blue-based-dye-for-donor-preparation-and-transplantation-in-descemet-membrane-endothelial-keratoplasty
#13
Sebastian Siebelmann, Mario Matthaei, Robert Hörster, Claus Cursiefen, Björn Bachmann
PURPOSE: Trypan blue (TB) is used for visualization of the endothelium-Descemet membrane graft during Descemet membrane endothelial keratoplasty (DMEK). However, TB is assumed to have a dose-dependent toxic effect on the corneal endothelium. We retrospectively compared a lutein/zeaxanthin and Brilliant Blue (LZ/BB)-based dye for preparation and transplantation of the graft in DMEK to TB. METHODS: In 20 patients, a dye composed of 1% LZ /0.025% BB was used, and in 22 patients, 0...
January 11, 2017: Cornea
https://www.readbyqxmd.com/read/28069498/poly-glycerol-sebacate-poly-%C3%AE%C2%B5-caprolactone-blend-nanofibrous-scaffold-as-intrinsic-bio-and-immunocompatible-system-for-corneal-repair
#14
Sahar Salehi, Marta Czugala, Piotr Stafiej, Mohammadhossein Fathi, Thomas Bahners, Jochen S Gutmann, Bernhard B Singer, Thomas A Fuchsluger
: A major challenge in corneal tissue engineering and lamellar corneal transplantation is to develop synthetic scaffolds able to simulate the optical and mechanical properties of the native cornea. As a carrier, the graft scaffolds should provide the basis for anchorage, repair and regeneration. Although quite a number of scaffolds have been engineered to date, they have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the corneal extracellular matrix (ECM)...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28061402/sequentially-crosslinked-bioactive-hydrogels-as-nano-patterned-substrates-with-customizable-stiffness-and-degradation-for-corneal-tissue-engineering-applications
#15
Muhammad Rizwan, Gary S L Peh, Heng-Pei Ang, Nyein Chan Lwin, Khadijah Adnan, Jodhbir S Mehta, Wui Siew Tan, Evelyn K F Yim
Naturally-bioactive hydrogels like gelatin provide favorable properties for tissue-engineering but lack sufficient mechanical strength for use as implantable tissue engineering substrates. Complex fabrication or multi-component additives can improve material strength, but often compromises other properties. Studies have shown gelatin methacrylate (GelMA) as a bioactive hydrogel with diverse tissue growth applications. We hypothesize that, with suitable material modifications, GelMA could be employed for growth and implantation of tissue-engineered human corneal endothelial cell (HCEC) monolayer...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/28060073/variable-size-bead-layer-as-standard-reference-for-endothelial-microscopes
#16
Simona Tufo, Erica Prazzoli, Lorenzo Ferraro, Federica Cozza, Alessandro Borghesi, Silvia Tavazzi
PURPOSE: For morphometric analysis of the cell mosaic of corneal endothelium, checking accuracy and precision of instrumentation is a key step. In this study, a standard reference sample is proposed, developed to reproduce the cornea with its shape and the endothelium with its intrinsic variability in the cell size. METHODS: A polystyrene bead layer (representing the endothelium) was deposited on a lens (representing the cornea). Bead diameters were 20, 25, and 30 μm (fractions in number 55%, 30%, and 15%, respectively)...
February 2017: Cornea
https://www.readbyqxmd.com/read/28009792/clinical-features-in-children-with-posterior-polymorphous-corneal-dystrophy
#17
Ye Jin Ahn, Soon Il Choi, Hae Ri Yum, Sun Young Shin, Shin Hae Park
PURPOSE: To describe clinical features in children diagnosed with posterior polymorphous corneal dystrophy (PPCD) in their first or second decade of life. METHODS: A retrospective study was performed with the medical records of seven unrelated Korean pediatric patients who were diagnosed with PPCD and were followed up for a minimum of 3 years. Thorough ocular examinations were performed, including best-corrected visual acuity, intraocular pressure, refractive and keratometric measurements, slit-lamp biomicroscopy, and specular microscopy at all visits...
December 22, 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/28002844/activation-of-the-rho-rho-kinase-signaling-pathway-is-involved-in-cell-death-of-corneal-endothelium
#18
Naoki Okumura, Keita Fujii, Takato Kagami, Nakahara Makiko, Miu Kitahara, Shigeru Kinoshita, Noriko Koizumi
Purpose: Rho kinase (ROCK) pathways control fundamental cell functions, making ROCK an important therapeutic target in several pathophysiologic conditions. The purpose of this study was to investigate whether inhibition of ROCK can suppress apoptosis of the corneal endothelium and to determine the role of ROCK signaling in regulating apoptosis. Methods: The effects of inhibitors of ROCK or myosin light chain (MLC) were evaluated in cultured monkey corneal endothelial cells (MCECs) irradiated with ultraviolet (UV) (100 J/m2) to induce apoptosis...
December 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27994805/descemet-s-membrane-endothelial-keratoplasty-in-south-asian-population
#19
Vipul Bhandari, Jagdeesh K Reddy, Prateek Chougale
PURPOSE: To evaluate visual outcomes, endothelial cell density and complications following Descemet's membrane endothelial keratoplasty (DMEK). METHODS: This study included 40 consecutive eyes that underwent DMEK for various pathologies involving the corneal endothelium. Best corrected visual acuity (BCVA) and endothelial cell density (ECD) were measured and compared before and 6 months after surgery. RESULTS: Out of 40 eyes, 34 eyes (85%) had BCVA ranging from 0...
October 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27986387/an-observational-cross-sectional-study-on-the-corneal-endothelium-of-medium-term-rigid-gas-permeable-contact-lens-wearers
#20
Michael J Doughty
PURPOSE: To assess if polymegethism and pleomorphism were evident in corneal endothelium after medium-term rigid gas permeable (RGP) contact lens wear. METHODS: In a cross-sectional observational study over 12 years, single images of the central region of the corneal endothelium of one eye of 46 subjects were taken with a non-contact specular microscope, along with a measure of central corneal thickness (CCT). The images were printed onto A3-sized paper and 100 cells/image measured by planimetry...
December 13, 2016: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
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