keyword
MENU ▼
Read by QxMD icon Read
search

Corneal endothelium

keyword
https://www.readbyqxmd.com/read/29656016/riding-the-cell-jamming-boundary-geometry-topology-and-phase-of-human-corneal-endothelium
#1
Nigel H Brookes
It is important to assess the viability of eye-banked corneas prior to transplantation due to inherent senescence and known loss of endothelial cells during surgical manipulation. Corneal endothelial cells have a complex basal and paracellular shape making them challenging to accurately measure, particularly in oedematous ex vivo tissue. This study used calibrated centroidal Voronoi Diagrams to segment cells in images of these human corneas, in order to characterize endothelial geometry, topology, and phase...
April 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29625488/a-cell-culture-approach-to-optimized-human-corneal-endothelial-cell-function
#2
Alena Bartakova, Olga Kuzmenko, Karen Alvarez-Delfin, Noelia J Kunzevitzky, Jeffrey L Goldberg
Purpose: Cell-based therapies to replace corneal endothelium depend on culture methods to optimize human corneal endothelial cell (HCEC) function and minimize endothelial-mesenchymal transition (EnMT). Here we explore contribution of low-mitogenic media on stabilization of phenotypes in vitro that mimic those of HCECs in vivo. Methods: HCECs were isolated from cadaveric donor corneas and expanded in vitro, comparing continuous presence of exogenous growth factors ("proliferative media") to media without those factors ("stabilizing media")...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29617399/activation-of-human-macrophages-by-human-corneal-allogen-in-vitro
#3
Paola Kammrath Betancor, Antonia Hildebrand, Daniel Böhringer, Florian Emmerich, Günther Schlunck, Thomas Reinhard, Thabo Lapp
PURPOSE: To study distinct aspects of human monocyte-derived macrophage (MDM) activation by human corneal tissue as a possible initial stage in human corneal allograft rejection. METHODS: Human monocytes were isolated from peripheral blood mononuclear cells (PBMC) and differentiated into MDM. Human corneas with or without endothelium were fragmented using a standardized protocol. MDM were stimulated with human corneal fragments, corneal fragment supernatant, lipopolysaccharide (LPS) or interferon-gamma (IFNγ), and expression profiles for 34 cytokines were determined in MDM-conditioned media using a Luminex bead-based multiplex assay...
2018: PloS One
https://www.readbyqxmd.com/read/29569838/the-feasibility-study-of-an-in-situ-marine-polysaccharide-based-hydrogel-as-the-vitreous-substitute
#4
Xiaolei Jiang, Yanfei Peng, Chaozhong Yang, Wanshun Liu, Baoqin Han
Although various biopolymers and synthetic compounds have been proposed and tested as the vitreous substitutes, no ideal material has been identified yet. In the present study, we developed an in situ-formed hydrogel by crosslinking hydroxypropyl chitosan (HPCTS) with alginate dialdehyde (ADA). Physical properties of the hydrogel were studied and the cytotoxicity was evaluated using L929 fibroblasts, rabbit corneal endothelial cells and retinal pigment epithelial cells. In a preliminary in vivo study, the hydrogel was employed as vitreous substitute after vitrectomy surgery on rabbits and multiple parameters indicating biosafety and biocompatibility were measured and analyzed postoperatively...
March 23, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29543663/role-of-dendritic-cells-and-inflammatory-cells-in-herpetic-endotheliitis-analysis-using-in-vivo-confocal-microscopy
#5
Ting Wang, Muchen Dong, Yin Jiang, Shuting Wang, Weiyun Shi
PURPOSE: To observe the pathological changes in dendritic cells (DCs) and inflammatory cells in the corneal epithelium and endothelium using in vivo confocal microscopy during the management of herpetic endotheliitis. METHODS: A total of 110 eyes with herpetic endotheliitis were included. All patients were treated with antiviral agents combined with glucocorticoids. Changes in corneal edema were observed using slit-lamp microscopy and anterior segment optical coherence tomography...
March 14, 2018: Cornea
https://www.readbyqxmd.com/read/29535350/tgf-%C3%AE-1-promotes-cell-barrier-function-upon-maturation-of-corneal-endothelial-cells
#6
Véronique Beaulieu Leclerc, Olivier Roy, Kim Santerre, Stéphanie Proulx
Human corneal endothelial cells (HCECs) easily become fibroblastic-like when cultured, rendering them unsuitable for tissue engineering of the cornea. Transforming growth factor β (TGF-β) could be a key factor in this phenomenon; however, TGF-β is also known to maintain the endothelium in a quiescent state in vivo. This work aimed to compare the effects of TGF-β1 on the phenotype of HCECs during the proliferation and maturation phases. Our results show that addition of TGF-β1 during the active proliferation phase produced fibroblastic HCECs and loss of the cell junction markers ZO-1 and n-cadherin, independent from the presence of epidermal growth factor (EGF)...
March 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29532272/publisher-correction-to-effect-of-glaucoma-implant-surgery-on-intraocular-pressure-reduction-flare-count-anterior-chamber-depth-and-corneal-endothelium-in-primary-open-angle-glaucoma
#7
Kyoko Ishida, Naoya Moroto, Kazuhiro Murata, Tetsuya Yamamoto
The publisher regrets that the original version.
March 12, 2018: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29531856/human-corneal-endothelial-cell-transplantation-using-nanocomposite-gel-sheet-in-bullous-keratopathy
#8
Periasamy Parikumar, Kazutoshi Haraguchi, Rajappa Senthilkumar, Samuel Jk Abraham
Transplantation of in vitro expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine's cornea has been earlier reported. Herein we report the transplantation of HCEP cells derived from a cadaver donor cornea to three patients using the NC gel sheet. In three patients with bullous keratopathy, one after cataract surgery, one after trauma and another in the corneal graft, earlier performed for congenital corneal dystrophy, not amenable to medical management HCEP cells isolated from a human cadaver donor cornea in vitro expanded using a thermoreversible gelation polymer (TGP) for 26 days were divided into three equal portions and 1...
2018: American Journal of Stem Cells
https://www.readbyqxmd.com/read/29529022/proteomic-analysis-of-corneal-endothelial-cell-descemet-membrane-tissues-reveals-influence-of-insulin-dependence-and-disease-severity-in-type-2-diabetes-mellitus
#9
Jessica M Skeie, Benjamin T Aldrich, Andrew S Goldstein, Gregory A Schmidt, Cynthia R Reed, Mark A Greiner
The objective of this study was to characterize the proteome of the corneal endothelial cell layer and its basement membrane (Descemet membrane) in humans with various severities of type II diabetes mellitus compared to controls, and identify differentially expressed proteins across a range of diabetic disease severities that may influence corneal endothelial cell health. Endothelium-Descemet membrane complex tissues were peeled from transplant suitable donor corneas. Protein fractions were isolated from each sample and subjected to multidimensional liquid chromatography and tandem mass spectrometry...
2018: PloS One
https://www.readbyqxmd.com/read/29521691/feasibility-study-of-human-corneal-endothelial-cell-transplantation-using-an-in-vitro-human-corneal-model
#10
Kostadin Rolev, Dominic G OʼDonovan, Peter Coussons, Linda King, Madhavan S Rajan
PURPOSE: To test the feasibility of a cell therapy approach to treat corneal endothelial (CE) disorders using an in vitro model of human corneal decompensation. METHODS: A CE decompensation model was established by removal of the Descemet membrane/endothelium complex from cadaveric human corneas in an air interface organ culture system (group 2) and compared with normal corneas (group 1). The posterior stroma of decompensated corneas was seeded with immortalized human corneal endothelial cells (HCEC-12) in group 3 and passage 0 primary human CE cells in group 4 corneas...
March 6, 2018: Cornea
https://www.readbyqxmd.com/read/29521294/effects-of-adipose-derived-mesenchymal-stem-cell-exosomes-on-corneal-stromal-fibroblast-viability-and-extracellular-matrix-synthesis
#11
Ting Shen, Qing-Qing Zheng, Jiang Shen, Qiu-Shi Li, Xing-Hui Song, Hong-Bo Luo, Chao-Yang Hong, Ke Yao
Background: Corneal stromal cells (CSCs) are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells (ADSCs) have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear...
March 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29516051/endothelial-keratoplasty-for-fuchs-dystrophy
#12
Laura Macovei, Ioana Gobej
We report the case of a 69-year-old female with Fuchs endothelial dystrophy and posterior chamber in-the-bag intraocular lens, whom we treated with DMEK surgical technique. We encountered difficulties both during obtaining the endothelium from the young donor and during the intraocular unrolling and its application on the stroma. We evaluated both preoperative and postoperative the following parameters: visual acuity, slit lamp, and optical coherence tomography appearance of the cornea, corneal thickness, intraocular pressure, endothelial cell density...
October 2017: Romanian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29516044/endothelial-cells-loss-to-the-hyperopic-pacients-during-phacoemulsification
#13
Dan-Mircea Stănilă, Andreea-Maria Florea, Adriana Stănilă, Alina Adriana Panga
Introduction: The phacoemulsification cataract surgery is the most frequently performed surgery and it generally improves vision in over 90% of the patients. Hyperopic patients are a challenge during phacoemulsification especially because of their short eyeball and shallow anterior chamber. A shallow anterior chamber is associated with overall reduction of the safe zone, which may lead to difficulty in creating the corneal incisions, harder capsulorhexis performing, or endothelial complications...
October 2017: Romanian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29499165/ectopic-grhl2-expression-due-to-non-coding-mutations-promotes-cell-state-transition-and-causes-posterior-polymorphous-corneal-dystrophy-4
#14
Petra Liskova, Lubica Dudakova, Cerys J Evans, Karla E Rojas Lopez, Nikolas Pontikos, Dimitra Athanasiou, Hodan Jama, Josef Sach, Pavlina Skalicka, Viktor Stranecky, Stanislav Kmoch, Caroline Thaung, Martin Filipec, Michael E Cheetham, Alice E Davidson, Stephen J Tuft, Alison J Hardcastle
In a large family of Czech origin, we mapped a locus for an autosomal-dominant corneal endothelial dystrophy, posterior polymorphous corneal dystrophy 4 (PPCD4), to 8q22.3-q24.12. Whole-genome sequencing identified a unique variant (c.20+544G>T) in this locus, within an intronic regulatory region of GRHL2. Targeted sequencing identified the same variant in three additional previously unsolved PPCD-affected families, including a de novo occurrence that suggests this is a recurrent mutation. Two further unique variants were identified in intron 1 of GRHL2 (c...
March 1, 2018: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29489516/five-keratoplasties-from-one-donor-cornea
#15
Oganes G Oganesyan, Vladimir V Neroev, Arthur A Grdikanyan, Vostan R Getadaryan
PURPOSE: To describe the feasibility of same-day, consecutive use of a single donor cornea in 5 patients with corneal pathology, who required anterior lamellar and endothelial keratoplasties. METHODS: Descemet membrane (DM) with endothelium was completely stripped from a single corneoscleral button and punched out from the endothelial side with a circular, standard punch. Using a custom-made punch for linear cutting of DM, we obtained 4 quarter-grafts (Q-graft)...
February 27, 2018: Cornea
https://www.readbyqxmd.com/read/29480366/inflammatory-cytokine-tnf-%C3%AE-promotes-corneal-endothelium-apoptosis-via-upregulating-tipe2-transcription-during-corneal-graft-rejection
#16
Qun Wang, Chao Wei, Li Ma, Xin Wang, Lin Li, Qingjun Zhou, Weiyun Shi
PURPOSE: Endothelial dysfunction accounts for 50% of total corneal transplantation failures, suggesting that corneal endothelial damage is the leading cause of graft failure. Tumor necrosis factor-α (TNF-α) is known to contribute to the negative regulation of corneal transplantation, but how it does so remains unclear. Here, we report a regulatory loop involving TNF-α, TNF-α-induced protein 8 like 2 (TNFAIP8L2 or TIPE2), and apoptosis during corneal graft rejection. METHODS: We established mice models of penetrating keratoplasty to verify whether the quantification of TNF-α in allogeneic corneas is enhanced through ELISA assay and immunofluorescence staining...
February 26, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29476773/a-potential-role-for-eph-receptor-signalling-during-migration-of-corneal-endothelial-cells
#17
Jennifer Walshe, Neil A Richardson, Najla Khaled Al Abdulsalam, Sally-Anne Stephenson, Damien G Harkin
The corneal endothelium is a monolayer of epithelial cells that lines the posterior surface of the cornea and is essential for maintenance of corneal transparency. Wound healing within the corneal endothelium typically occurs through cell spreading and migration rather than through proliferation. The mechanisms that control corneal endothelial cell migration are unclear. In this study we demonstrate that cultures of corneal endothelial cells display reduced migration in scratch wound assays, and reduced levels of E-cadherin mRNA, following suppression of ligand-activated Eph receptor signalling by treatment with lithocholic acid...
February 21, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29474308/risk-factors-treatment-strategies-and-outcomes-of-endophthalmitis-associated-with-severe-fungal-keratitis
#18
Lei Wan, Jun Cheng, Jing Zhang, Nan Chen, Yan Gao, Li-Xin Xie
PURPOSE: To investigate risk factors, treatment strategies, and outcomes of endophthalmitis associated with severe fungal keratitis. METHODS: Data from 392 patients diagnosed with fungal keratitis were retrospectively examined. Patients had severe disease that was refractory to topical or systemic antifungal therapy, and the infection involved the endothelium or perforated the cornea. The incidence and risk factors for endophthalmitis, and treatment outcomes were evaluated...
February 22, 2018: Retina
https://www.readbyqxmd.com/read/29468215/descemet-membrane-endothelial-keratoplasty-for-corneal-decompensation-due-to-iridoschisis
#19
Miles F Greenwald, Philip I Niles, A Tim Johnson, Jesse M Vislisel, Mark A Greiner
Purpose: To report a case of bilateral iridoschisis with cataracts and corneal decompensation in a patient who underwent cataract extraction and superficial iridectomy followed by Descemet membrane endothelial keratoplasty (DMEK). Observations: A 58-year-old man with previously diagnosed iridoschisis, cataracts, and diabetes mellitus experienced progressive vision loss bilaterally due to corneal decompensation. Slit lamp examination revealed iridoschisis with iris fibrils contacting the corneal endothelium, stromal edema, and mild guttate changes bilaterally...
March 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29452452/-effectivity-and-safety-of-a-modified-tip-design-in-torsional-phacoemulsification
#20
Sabine Schmidt, Sophie Hubich, Jan Markus Vetter, Christopher Wirbelauer
BACKGROUND: Torsional mode phacoemulsification results in more effective fragmentation of the nucleus due to a different movement of the phacotip. In this clinical study, we investigated the influence of a modified tip design and active fluidics on the efficacy of phacoemulsification and safety for the corneal endothelium. MATERIAL AND METHODS: We conducted a prospective randomized 2 : 1 study in which 40 patients were operated on with the mini-flared Kelman Tip using the Infiniti ® System (group 1), and 20 patients were operated on with the Intrepid ® Balanced Tip and the Centurion ® System...
February 16, 2018: Klinische Monatsblätter Für Augenheilkunde
keyword
keyword
65430
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"