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Corneal endothelium

Sabine Schmidt, Sophie Hubich, Jan Markus Vetter, Christopher Wirbelauer
BACKGROUND: Torsional mode phacoemulsification results in more effective fragmentation of the nucleus due to a different movement of the phacotip. In this clinical study, we investigated the influence of a modified tip design and active fluidics on the efficacy of phacoemulsification and safety for the corneal endothelium. MATERIAL AND METHODS: We conducted a prospective randomized 2 : 1 study in which 40 patients were operated on with the mini-flared Kelman Tip using the Infiniti ® System (group 1), and 20 patients were operated on with the Intrepid ® Balanced Tip and the Centurion ® System...
February 16, 2018: Klinische Monatsblätter Für Augenheilkunde
Stefan A Kassumeh, Christian M Wertheimer, Annabel von Studnitz, Anna Hillenmayer, Claudia Priglinger, Armin Wolf, Wolfgang J Mayer, Daniel Teupser, Lesca M Holdt, Siegfried G Priglinger, Kirsten H Eibl-Lindner
PURPOSE: Posterior capsule opacification (PCO) still represents the main long-term complication of cataract surgery. Research into pharmacologic PCO prophylaxis is extensive. One promising candidate drug is methotrexate (MTX). Our aim is to determine the in vitro feasibility of MTX-loaded poly(lactic-co-glycolic) (PLGA) biomatrices sprayed on intraocular lenses (IOLs) as a drug-delivery implant. METHODS: Hydrophilic and hydrophobic acrylic IOLs were spray-coated with MTX-loaded PLGA...
February 16, 2018: Current Eye Research
Ahmad Mirshahi, Alireza Lashay, Mohammad Reza Mehrabi Bahar, Mojtaba Abrishami
Background: Mitomycin C (MMC) as an alkylating agent is miscellaneous an antineoplastic, antibiotic and ophthalmic agent. Here we aim to report a case of inadvertent intravitreal MMC injection instead of Avastin in case of diabetic macular edema. Case presentation: A 53 years old woman was planned to receive intravitreal Avastin injection, but accidentally, 0.05 ml of MMC 0.2% was injected. Best corrected visual acuity (BCVA) was 20/160 before injection. After 2 days, patient was referred to a tertiary referral eye center...
2018: International Journal of Retina and Vitreous
Kate E Leahy, Michele C Madigan, Maria Sarris, Stephanie L Watson, Peter McCluskey, Andrew Jr White
IMPORTANCE: Transient corneal endothelial changes are routinely noted on slit-lamp examination immediately following selective laser trabeculoplasty (SLT). BACKGROUND: To determine the mechanism of the transient corneal endothelial changes observed following SLT. DESIGN: University laboratory based observational study SAMPLES: Ten corneas from six human cadaveric donors. METHODS: Corneas were treated with SLT, direct laser, or peroxide, or used as controls...
February 14, 2018: Clinical & Experimental Ophthalmology
Bert Van den Bogerd, Sorcha Ní Dhubhghaill, Nadia Zakaria
The idea of transplanting a sheet of laboratory-grown corneal endothelium dates back to 1978; however, the ideal scaffold is still lacking. We hypothesized that human crystalline lens capsules (LCs) could qualify as a scaffold and aimed to characterize the properties of this material for endothelial tissue engineering. LCs were isolated from donor eyes, stored at -80 °C, and decellularized with water and trypsin-EDTA. The decellularization was investigated by nuclear staining and counting and the capsule thickness was determined by optical coherence tomography and compared with Descemet's membrane (DM)...
December 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
T Huang, L Jiang, J Zhan, C Ouyang
Objective: To investigate the clinical outcomes of Pre-descement membrane endothelial keratoplasty (PDEK) for treatment of patients with corneal endothelial decompensation. Methods: Retrospective study of case series. The cases of 20 patients (20 eyes) who were diagnosed with corneal endothelial decompensation induced by various original diseases and received PDEK during July 2016 and December 2016 at Zhongshan Ophthalmic Center have been analyzed. The participants included 8 males and 12 females with an average age of (59...
February 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Zhenzhen Yan, Haihong Shi, Rongrong Zhu, Lele Li, Bai Qin, Lihua Kang, Hui Chen, Huaijin Guan
Purpose: Age-related macular degeneration (AMD) is the leading cause of central visual loss among patients over the age of 55 years worldwide. Neovascular-type AMD (nAMD) accounts for approximately 10% of patients with AMD and is characterized by choroidal neovascularization (CNV). The proliferation of choroidal endothelial cells (CECs) is one important step in the formation of new vessels. Transcriptional coactivator Yes-associated protein (YAP) can promote the proliferation of multiple cancer cells, corneal endothelial cells, and vascular smooth muscle cells, which participate in angiogenesis...
2018: Molecular Vision
Pramod Kumar Sahu, Gopal Krushna Das, Sumeet Agrawal, Sabitabh Kumar, Nitish Kumar
PURPOSE: To determine if phacoemulsification with intraocular lens implantation has a greater impact on the corneal endothelium of type 2 diabetic patients as compared to nondiabetics. MATERIALS AND METHODS: This study compared the endothelial status in 60 diabetics with good glycemic control and 60 nondiabetics before and after uneventful phacoemulsification. Central corneal endothelial cell density, percentage hexagonality and percentage coefficient of variation (%CV) were measured using a specular microscope...
October 2017: Middle East African Journal of Ophthalmology
Do Kyung Kim, Bo Ra Sim, Jeong In Kim, Gilson Khang
Design of corneal endothelium substitute is important for replacement of cadaveric cornea tissue. Our previous study has shown the suitability of silk fibroin (SF) as a biomaterial for cornea scaffold. In this study, we used β-Carotene (β-C) to enhance the regeneration of corneal endothelial cells (CEnCs) and maintain CEnC specific function. The fabricated film scaffolds showed desired transparency and hydrophilic properties which are crucial factors for vision recovery. The cell viability, phenotype and gene expression was examined using MTT assay, immunofluorescence and reverse transcription polymerase chain reactions...
November 22, 2017: Colloids and Surfaces. B, Biointerfaces
Sabrina Chebli, Muriel Rabilloud, Carole Burillon, Viridiana Kocaba
PURPOSE: To evaluate the long-term endothelial tolerance of iris-fixated phakic intraocular lenses (pIOLs) over a 10-year period, to identify predictive factors for endothelial decompensation, and to develop a model to predict endothelial cell survival after pIOL implantation. METHODS: This retrospective study included 130 eyes in 60 patients who underwent pIOL implantation for correction of high myopia from January 2003 to October 2012 at Edouard Herriot Hospital in Lyon, France...
February 2, 2018: Cornea
Sergi Segarra, Marta Leiva, Daniel Costa, Natàlia Coyo, Maria Sabés-Alsina, José Ríos, Teresa Peña
BACKGROUND: Rabbits are currently not a good model for studying diseases of the corneal endothelium because their corneal endothelial cells (CECs) maintain a high proliferative capacity throughout almost all their life. Addressing this particular feature might allow the use of this species for such a purpose. The aim of this study was to evaluate the corneal endothelial injury after intracameral benzalkonium chloride (BAC) injection into rabbit eyes ex vivo, and to establish the most suitable starting dose for an in vivo study aimed at developing an animal model of corneal endothelial disease...
February 2, 2018: BMC Veterinary Research
Jonathan H Lass, Beth Ann Benetz, Harry J Menegay, Constantinos P Tsipis, Jane Caty Cook, David S Boyer, Michael Singer, Kristine Erickson, Namrata Saroj, Robert Vitti, Karen W Chu, Hadi Moini, Yuhwen Soo, Yenchieh Cheng
PURPOSE: The effects of repeated intravitreal aflibercept injection (IAI) on the corneal endothelium were studied in patients with unilateral neovascular age-related macular degeneration. METHODS: RE-VIEW was a phase 4, open-label, single-arm, multicenter study. Patients received IAI every 8 weeks after 3 monthly doses. Slit-lamp biomicroscopy was performed at all study visits. The central corneal endothelial health was evaluated by specular microscopy in the treated versus untreated fellow eyes at baseline and weeks 24 and 52...
January 30, 2018: Cornea
Naoki Okumura, Takato Kagami, Keita Fujii, Makiko Nakahara, Noriko Koizumi
PURPOSE: The cell-cell adhesion molecules, cadherins and nectins, are involved in the formation of adherens junctions. However, involvement of nectins in the corneal endothelium has not yet been established. This study investigated the involvement of nectins in adherens junctions of the corneal endothelium. METHODS: Nectin and cadherin expression in the corneal endothelium was evaluated by real-time polymerase chain reaction. Colocalization and direct binding of nectin-1 and N-cadherin to anchoring proteins (afadin and β-catenin, respectively) were determined by immunostaining and immunoprecipitation...
January 30, 2018: Cornea
Yu Qiang Soh, Jodhbir S Mehta
PURPOSE: Fuchs endothelial corneal dystrophy (FECD) is an acquired corneal endotheliopathy and is one of the most common indications for corneal transplantation surgery worldwide. Endothelial keratoplasty (EK) is the most popular form of corneal transplantation for FECD. In standard EK surgery, the patient's corneal endothelium and basement membrane [ie, Descemet membrane (DM)] are first removed, followed by transplantation of donor tissue that comprises allogenic corneal endothelial cells, DM, and corneal stroma of variable thickness...
January 30, 2018: Cornea
Hassan Hamoudi
No abstract text is available yet for this article.
January 2018: Acta Ophthalmologica
Prashant Garg
No abstract text is available yet for this article.
February 2018: Indian Journal of Ophthalmology
Neha Kapur, Sridevi Gunda, Surender Dixit, Lokesh Chauhan, Manisha Acharya, Umang Mathur
PURPOSE: The purpose of this study is to evaluate the effect of transfer of donor corneal tissue from McCarey-Kaufmann (MK) medium to Optisol-GS on corneal endothelium. METHODS: This was a prospective, randomized comparative study. Twenty paired human donor corneal tissues of optical quality were retrieved. One tissue of the pair was preserved in Optisol-GS preservative medium (Group A) and other tissue of the pair in MK medium (Group B) at the time of corneoscleral disc excision...
February 2018: Indian Journal of Ophthalmology
Jiwoong Park, Myungwon Lee
BACKGROUND: The purpose of this study is to evaluate short-term effects and safety of an acute increase of intraocular pressure (IOP) after single-dose intravitreal bevacizumab injection on corneal endothelial cells and central corneal thickness. METHODS: Forty-two patients who underwent intravitreal injection of 2.5 mg/0.1 ml bevacizumab because of central serous chorioretinopathy or diabetic macular edema were included in this study. The changes of IOP, corneal endothelial cells, and corneal thickness at baseline, 2 min, 5 min, and 30 min after injection were analyzed prospectively with a specular microscope...
January 25, 2018: BMC Ophthalmology
Joni A Turunen, Juho Wedenoja, Pauliina Repo, Reetta-Stiina Järvinen, Johannes E Jäntti, Sanna Mörtenhumer, Antti S Riikonen, Anna-Elina Lehesjoki, Anna Majander, Tero T Kivelä
PURPOSE: To describe the phenotype and the genetic defect in keratoendotheliitis fugax hereditaria, an autosomal dominant keratitis that periodically affects the corneal endothelium and stroma, leading in some patients to opacities and decreased visual acuity. DESIGN: Cross-sectional, hospital-based study. METHODS: Patient population: Thirty affected and 7 unaffected subjects from 7 families, and 4 sporadic patients from Finland. OBSERVATION PROCEDURES: Ophthalmic examination and photography, corneal topography, specular microscopy and optical coherence tomography in 34 patients, whole exome sequencing in 10 patients, and Sanger sequencing in 34 patients...
January 20, 2018: American Journal of Ophthalmology
JeongGoo Lee, Eric Jung, Martin Heur
Investigating stimulation of endogenous wound healing in corneal endothelial cells (CECs) may help address the global shortage of donor corneas by decreasing the number of transplants performed for blindness due to endothelial dysfunction.  We previously reported that IL-1β stimulation leads to fibroblast growth factor (FGF2) expression, enhancing migration and proliferation of mammalian CECs.  However, FGF2 also promotes the endothelial-mesenchymal transition, which can lead to retrocorneal membrane formation and blindness...
January 23, 2018: Journal of Biological Chemistry
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