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https://www.readbyqxmd.com/read/29148584/metabolic-effects-of-p-p-dde-on-atlantic-salmon-hepatocytes
#1
Pål A Olsvik, Liv Søfteland
Decades after being banned in many countries, DDT and its metabolites are still considered major environmental hazards. The p,p'-DDE isomer, the DDT metabolite found in highest concentration in aquaculture feeds, is an endocrine disruptor with demonstrated ability to induce epigenetic effects. This study aimed at examining the impact of p,p'-DDE on Atlantic salmon. Primary hepatocytes were exposed to four concentrations of p,p'-DDE (0.1, 1, 10, 100 μm) for 48 hours, and endpoints included cytotoxicity, global DNA methylation, targeted transcription and metabolomics profiling (100 μm)...
November 17, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29145706/expression-of-oestrogen-receptors-gper-esr1-esr2-in-human-ductuli-efferentes-and-proximal-epididymis
#2
V Rago, F Romeo, F Giordano, R Malivindi, V Pezzi, I Casaburi, A Carpino
Oestrogen targeting in the human genital ducts is still not well-known. In fact, to date, the localization of oestrogen receptors, ESR1 and ESR2, is controversial and the presence of the membrane oestrogen receptor GPER (G protein-coupled oestrogen receptor) is unexplored. This study has investigated the expression of GPER, ESR1, ESR2 in human ductuli efferentes and proximal caput epididymis by immunohistochemistry and Western blot analysis. Furthermore, the presence of PELP1 (proline-glutamic acid-leucine-rich protein 1), a co-regulator of the oestrogen receptors, was also evaluated...
November 16, 2017: Andrology
https://www.readbyqxmd.com/read/29145598/pubertal-escape-from-estradiol-negative-feedback-in-ewe-lambs-is-not-accounted-for-by-decreased-esr1-mrna-or-protein-in-kisspeptin-neurons
#3
Michelle N Bedenbaugh, Marcella D'Oliveira, Rodolfo C Cardoso, Stanley M Hileman, Gary L Williams, Marcel Amstalden
In this study, we investigated whether decreased sensitivity to estradiol negative feedback is associated with reduced ESR1 expression in kisspeptin neurons as ewe lambs approach puberty. Lambs were ovariectomized and received no implant (OVX) or an implant containing estradiol (OVX+E). In the middle arcuate nucleus (mARC), ESR1 mRNA was greater in OVX than OVX+E lambs but did not differ elsewhere. Posthoc analysis of LH secretion from OVX+E lambs revealed three patterns of LH pulsatility: low (1-2 pulses/12 h; LF, n=3), moderate (6-7 pulses/12 h; MF, n=6) and high (>10 pulses/12 h; HF, n=5)...
November 14, 2017: Endocrinology
https://www.readbyqxmd.com/read/29137354/erps294-is-a-biomarker-of-ligand-or-mutational-er%C3%AE-activation-and-a-breast-cancer-target-for-cdk2-inhibition
#4
Gary K Scott, David Chu, Ravneet Kaur, Julia Malato, Daniel E Rothschild, Katya Frazier, Serenella Eppenberger-Castori, Byron Hann, Ben Ho Park, Christopher C Benz
ERα phosphorylation at hinge site S294 (pS294) was recently shown to be essential for ER-dependent gene transcription and mediated by an unknown cyclin-dependent kinase (CDK). This study was undertaken to identify the exact CDK pathway mediating pS294 formation, and to determine if this phosphorylation event occurs with, and can be targeted to treat, the ligand-independent growth of breast cancers expressing endocrine-refractory ESR1 mutations. Using a newly developed anti-pS294 monoclonal antibody, a combination of CDK specific siRNA knockdown studies and a broad panel of CDK selective inhibitors against ligand (E2)-stimulated MCF7 cells, we first identified CDK2 as the primary mediator of pS294 formation and showed that CDK2-selective inhibitors like Dinaciclib, but not CDK4/6 inhibitors like Palbociclib, can selectively prevent pS294 formation and repress ER-dependent gene expression...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29133365/pre-diagnostic-smoking-is-associated-with-binary-and-quantitative-measures-of-er-protein-and-esr1-mrna-expression-in-breast-tumors
#5
Eboneé N Butler, Jeannette T Bensen, Mengjie Chen, Kathleen Conway, David B Richardson, Xuezheng Sun, Joseph Geradts, Andrew F Olshan, Melissa A Troester
Introduction Smoking is a possible risk factor for breast cancer and has been linked to increased risk of estrogen-receptor positive (ER+) disease in some epidemiologic studies. It is unknown whether smoking has quantitative effects on ER expression. Methods We examined relationships between smoking and ER expression from tumors of 1,888 women diagnosed with invasive breast cancer from a population-based study in North Carolina. ER expression was characterized using binary (+/-) and continuous measures for ER protein, ESR1 mRNA, and a multigene luminal score (LS) that serves as a measure of estrogen signaling in breast tumors...
November 13, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/29126187/med12-regulates-ovarian-steroidogenesis-uterine-development-and-maternal-effects-in-the-mammalian-egg
#6
Xinye Wang, Priya Mittal, Carlos A Castro, Gabriel Rajkovic, Aleksandar Rajkovic
The transcriptional factor MED12 is part of the essential mediator transcriptional complex that acts as a transcriptional coactivator in all eukaryotes. Missense gain of function mutations in human MED12 are associated with uterine leiomyomas, yet the role of MED12 deficiency in tumorigenesis and reproductive biology has not been fully explored. We generated a Med12 reproductive conditional knockout mouse model to evaluate its role in uterine mesenchyme, granulosa cells, and oocytes. Mice heterozygous for Med12 deficiency in granulosa cells and uterus (Med12fl/+ Amhr2-Cre) were subfertile, while mice homozygous for Med12 deficiency in granulosa cells and uterus (Med12fl/fl Amhr2-Cre) were infertile...
November 8, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29122055/extruded-linseed-alone-or-in-combination-with-fish-oil-modifies-mammary-gene-expression-profiles-in-lactating-goats
#7
Y Faulconnier, L Bernard, C Boby, J Domagalski, Y Chilliard, C Leroux
Nutrition is a major factor that regulates ruminant milk components, particularly its fatty acid (FA) composition, which is an important determinant of milk nutritional quality. In the mammary gland, milk component biosynthesis involves a large number of genes under nutritional regulation that are not well understood. Thus, the objective of the present study was to evaluate the effects of extruded linseeds (EL) alone or in combination with fish oil (ELFO) on goat mammary gene expression. In total, 14 goats were fed one of the following three diets: a natural grassland hay basal diet (CTRL) alone, CTRL supplemented with 530 g/day of EL, or 340 g/day of EL plus 39 g/day of fish oil (ELFO)...
November 10, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29118976/impact-of-hormonal-modulation-at-proestrus-on-ovarian-responses-and-uterine-gene-expression-of-suckled-anestrous-beef-cows
#8
Manoel Francisco de Sá Filho, Angela Maria Gonella-Diaza, Mariana Sponchiado, Marcio Ferreira Mendanha, Guilherme Pugliesi, Roney Dos Santos Ramos, Sónia Cristina da Silva Andrade, Gustavo Gasparin, Luiz Lehmann Coutinho, Marcelo Demarchi Goissis, Fernando Silveira Mesquita, Pietro Sampaio Baruselli, Mario Binelli
Background: This study evaluated the impact of hormonal modulation at the onset of proestrus on ovarian response and uterine gene expression of beef cows. Methods: A total of 172 anestrous beef cows were assigned to one of four groups according to the treatment with estradiol cypionate (ECP) and/or equine chorionic gonadotropin (eCG) [CON (n = 43), ECP (n = 43), eCG (n = 44) and ECP + eCG (n = 42)]. Results: ECP-treated cows (ECP and ECP + eCG groups) presented greater occurrence of estrus (44...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29115049/generation-and-characterization-of-an-estrogen-receptor-alpha-icre-knock-in-mouse
#9
Chan Jin Park, Guanglin Chen, Yongbum Koo, Po-Ching P Lin, Joseph A Cacioppo, Hailey Prohaska, CheMyong J Ko
Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and non-overlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1-iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells...
November 8, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/29103441/enpp1-and-esr1-genotypes-associated-with-subclassifications-of-craniofacial-asymmetry-and-severity-of-temporomandibular-disorders
#10
Kay Chung, Tabitha Richards, Romain Nicot, Alexandre R Vieira, Christiane V Cruz, Gwénaël Raoul, Joel Ferri, James J Sciote
INTRODUCTION: We investigated whether ACTN3, ENPP1, ESR1, PITX1, and PITX2 genes which contribute to sagittal and vertical malocclusions also contribute to facial asymmetries and temporomandibular disorders (TMD) before and after orthodontic and orthognathic surgery treatment. METHODS: One hundred seventy-four patients with a dentofacial deformity were diagnosed as symmetric or subdivided into 4 asymmetric groups according to posteroanterior cephalometric measurements...
November 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/29102757/response-of-xenografts-of-developing-human-female-reproductive-tracts-to-the-synthetic-estrogen-diethylstilbestrol
#11
Gerald R Cunha, Takeshi Kurita, Mei Cao, Joel Shen, Stanley J Robboy, Laurence Baskin
Human female fetal reproductive tracts 9.5-22 weeks of gestation were grown for 1 month in ovariectomized athymic adult female mouse hosts that were either untreated or treated continuously with diethylstilbestrol (DES) via subcutaneous pellet. Normal morphogenesis and normal patterns of differentiation marker expression (KRT6, KRT7, KRT8, KRT10, KRT14, KRT19, ESR1, PGR, TP63, RUNX1, ISL1, HOXA11 and α-ACT2) were observed in xenografts grown in untreated hosts and mimicked observations of previously reported (Cunha et al...
October 4, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29102591/hippocampal-gene-expression-patterns-in-oxytocin-male-knockout-mice-are-related-to-impaired-social-interaction
#12
Virginia Meneghini Lazzari, Josi Maria Zimmermann-Peruzatto, Grasiela Agnes, Roberta Oriques Becker, Ana Carolina de Moura, Silvana Almeida, Renata Padilha Guedes, Marcia Giovenardi
Social interaction between animals is crucial for the survival and life in groups. It is well demonstrated that oxytocin (OT) and vasopressin (AVP) play critical roles in the regulation of social behaviors in mammals, however, other neurotransmitters and hormones are involved in the brain circuitry related to these behaviors. The present study aimed to investigate the gene expression of neurotransmitter receptors in the brain of OT knockout (OTKO) male mice. In this study, we evaluated the expression levels of the OT receptor (Oxtr), AVP receptors 1a and 1b (Avpr1a; Avpr1b), dopamine receptor 2 (Drd2), and the estrogen receptors alpha and beta (Esr1; Esr2) genes in the hippocampus (HPC), olfactory bulb (OB), hypothalamus (HPT) and prefrontal cortex (PFC)...
November 1, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29100665/sex-steroid-receptors-prostaglandin-e2-receptors-and-cyclooxygenase-in-the-equine-cervix-during-estrus-diestrus-and-pregnancy-gene-expression-and-cellular-localization
#13
Claudia B Fernandes, Shavahn C Loux, Kirsten E Scoggin, Edward L Squires, Mats H Troedsson, Alejandro Esteller-Vico, Barry A Ball
The cervix is a dynamic structure that undergoes dramatic changes during the estrous cycle, pregnancy and parturition. It is well established that hormonal changes, including estrogens, progestogens and prostaglandins, regulate the expression of key proteins involved in cervical function. The arachidonic acid cascade is important in the remodeling and relaxation of the cervix in the days preceding parturition. Despite the complexity of this mechanism, regulation of cervical function has received little study in the mare...
October 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29095075/suitable-reference-gene-selection-for-gene-expression-studies-in-knee-osteoarthritis-synovium-using-quantitative-pcr-analysis
#14
Hiroshi Watanabe, Hirotaka Ishii, Kenji Takahashi, Shinro Takai, Hitoshi Ozawa
Osteoarthritis (OA) is the leading cause of musculoskeletal disability in the elderly. Insights into the biological features of OA are obtained by characterization of the molecular features by gene expression profiling using reverse transcription-quantitative PCR (RT-qPCR). However, it has recently become evident that the use of suitable reference genes is required for appropriate normalization of this technique. Here total RNA was isolated from the synovium of 18 men and 20 women who underwent total knee arthroplasty for knee OA (KOA)...
November 2, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29093337/bisphenol-af-as-an-inducer-of-estrogen-receptor-%C3%AE-er%C3%AE-evidence-for-anti-estrogenic-effects-at-higher-concentrations-in-human-breast-cancer-cells
#15
Hiroyuki Okazaki, Shuso Takeda, Kazuhiro Kakizoe, Aya Taniguchi, Miki Tokuyasu, Taichi Himeno, Hiroyuki Ishii, Eriko Kohro-Ikeda, Koichi Haraguchi, Kazuhito Watanabe, Hironori Aramaki
Bisphenols are endocrine disruptors that are widely found in the environment. Accumulating experimental evidence suggests an adverse interaction between bisphenols and estrogen signaling. Most studies have performed experiments that focused on estrogen receptor (ER) engagement by bisphenols. Therefore, the effects of bisphenols on the expression of ERα (ESR1) and ERβ (ESR2) remain largely unknown. In the present study, we examined the effects of four bisphenols: bisphenol A (BPA), bisphenol B (BPB), bisphenol S (BPS), and bisphenol AF (BPAF), on estrogen signaling in two human breast cancer cell lines (MCF-7 and SK-BR-3)...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29092056/effects-of-exposure-to-the-endocrine-disrupting-chemical-bisphenol-a-during-critical-windows-of-murine-pituitary-development
#16
Kirsten S Eckstrum, Whitney Edwards, Annesha Banerjee, Wei Wang, Jodi A Flaws, John A Katzenellenbogen, Sung Hoon Kim, Lori T Raetzman
Critical windows of development are often more sensitive to endocrine disruption. The murine pituitary gland has two critical windows of development: embryonic gland establishment and neonatal hormone cell expansion. During embryonic development, one environmentally ubiquitous endocrine disrupting chemical (EDC), bisphenol A (BPA), has been shown to alter pituitary development by increasing proliferation and gonadotrope number in females, but not males. However, the effects of exposure during the neonatal period were not examined...
October 6, 2017: Endocrinology
https://www.readbyqxmd.com/read/29082740/methylation-status-of-the-estrogen-receptor-1-promoter-predicts-poor-prognosis-of-acute-on-chronic-hepatitis-b-liver-failure
#17
Xiao-Peng Fan, Cheng-Yun Dou, Yu-Chen Fan, Chuang-Jie Cao, Ze-Hua Zhao, Kai Wang
BACKGROUND: Acute-on-chronic hepatitis B liver failure (ACHBLF) is an acute deteriorating liver disease and rapidly progresses to multiple organ failure. There is currently no adequate accurate predictive models of ACHBLF prognosis. AIMS: To identify the methylation frequency of the estrogen receptor 1 (ESR1) promoter in ACHBLF and analyze the associated prognostic significance. METHODS: Methylation-specific PCR (MSP) was used to determine the methylation frequency of the ESR1 promoter in peripheral blood mononuclear cells from a training and validation cohort of patients...
October 30, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29082433/reproductive-affective-disorders-a-review-of-the-genetic-evidence-for-premenstrual-dysphoric-disorder-and-postpartum-depression
#18
REVIEW
Katherine McEvoy, Lauren M Osborne, Julie Nanavati, Jennifer L Payne
PURPOSE OF REVIEW: The purpose of this study is to review and summarize the literature exploring the genetic basis for premenstrual dysphoric disorder (PMDD) and postpartum depression (PPD). RECENT FINDINGS: There is more evidence for a genetic basis for PPD than for PMDD, but only when PPD is defined as beginning in the immediate postpartum time period. Familial, genome-wide linkage and association studies, and candidate gene studies, most in the past 10 years, have examined the genetic etiology of reproductive affective disorders, including PMDD and PPD...
October 30, 2017: Current Psychiatry Reports
https://www.readbyqxmd.com/read/29074841/a-network-pharmacology-approach-to-determine-the-active-components-and-potential-targets-of-curculigo-orchioides-in-the-treatment-of-osteoporosis
#19
Nani Wang, Guizhi Zhao, Yang Zhang, Xuping Wang, Lisha Zhao, Pingcui Xu, Dan Shou
BACKGROUND Osteoporosis is a complex bone disorder with a genetic predisposition, and is a cause of health problems worldwide. In China, Curculigo orchioides (CO) has been widely used as a herbal medicine in the prevention and treatment of osteoporosis. However, research on the mechanism of action of CO is still lacking. The aim of this study was to identify the absorbable components, potential targets, and associated treatment pathways of CO using a network pharmacology approach. MATERIAL AND METHODS We explored the chemical components of CO and used the five main principles of drug absorption to identify absorbable components...
October 27, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29069289/distinct-dynorphin-expression-patterns-with-low-and-high-dose-estrogen-treatment-in-the-arcuate-nucleus-of-female-rats
#20
Moeko Kanaya, Kinuyo Iwata, Hitoshi Ozawa
Kisspeptin (KISS1; encoded by Kiss1 ) neurons in the arcuate nucleus (ARC) co-express tachykinin 3 (TAC3; also known as neurokinin B) and dynorphin A (PDYN). Accordingly, they are termed KNDy neurons and considered to be crucial in generating pulsatile release of gonadotropin-releasing hormone. Accumulating evidence suggests that Kiss1 and Tac3 are negatively regulated by estrogen. However it has not been fully determined whether and how estrogen modulates Pdyn and PDYN. Here, we examined the expression of Pdyn mRNA and PDYN by in situ hybridization and immunohistochemistry, respectively, in the ARC of female rats after ovariectomy (OVX) and OVX plus low or high-dose beta-estradiol (E 2 ) replacement...
October 24, 2017: Biology of Reproduction
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