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https://www.readbyqxmd.com/read/24409690/-high-efficiency-l-lactate-production-from-glycerol-by-metabolically-engineered-escherichia-coli
#1
Kangming Tian, Guiyang Shi, Fuping Lu, Suren Singh, Zhengxiang Wang
High-efficient conversion of glycerol to L-lactate is beneficial for the development of both oil hydrolysis industry and biodegradable materials manufacturing industry. In order to construct an L-lactate producer, we first cloned a coding region of gene BcoaLDH encoding an L-lactate dehydrogenase from Bacillus coagulans CICIM B1821 and the promoter sequence (P(ldhA)) of the D-lactate dehydrogenase (LdhA) from Escherichia coli CICIM B0013. Then we assembled these two DNA fragments in vitro and yielded an expression cassette, P(ldhA)-BcoaLDH...
September 2013: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/20693877/influence-of-tidal-volume-on-pulse-pressure-variations-in-hypovolemic-ventilated-pigs-with-acute-respiratory-distress-like-syndrome
#2
COMPARATIVE STUDY
Claes U Wiklund, Denis R Morel, Hélène Orbring-Wiklund, Jacques-Andre Romand, Vincent Piriou, Jean-Louis Teboul, Karim Bendjelid
BACKGROUND: Sensitivity and specificity of respiratory change in pulse pressure (DeltaPP) to predict preload dependency has been questioned at small tidal volumes (VT) in critically ill patients suffering from acute respiratory distress syndrome (ARDS). We studied DeltaPP in pigs with ARDS-like syndrome during reversible hemorrhagic shock. METHODS: Prospective, observational animal study in a Laboratory Investigation Unit. Sixteen deeply sedated mechanically ventilated pigs were successively ventilated with VT of 10 ml/kg at a respiratory rate of 15 breaths/min (RR15) and VT of 6 ml/kg at RR15 and RR25...
September 2010: Anesthesiology
https://www.readbyqxmd.com/read/20458612/relations-between-respiratory-changes-in-r-wave-amplitude-and-arterial-pulse-pressure-in-mechanically-ventilated-patients
#3
Maxime Cannesson, Geoffray Keller, Olivier Desebbe, Jean-Jacques Lehot
INTRODUCTION: R-wave obtained from the electrocardiogram depends on ventricular stroke volume. We assessed the relationship between respiratory variations in R-wave (DeltaRDII) and in pulse pressure (DeltaPP) during general anesthesia. METHOD: R-wave amplitude was measured from standard lead II (RDII). Maximal RDII (RDIImax) and minimal RDII (RDIImin) were determined over one respiratory cycle. DeltaRDII was calculated as 100 x [RDIImax-RDIImin]/([RDIImax + RDIImin]/2...
June 2010: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/20130968/changes-in-r-wave-amplitude-in-dii-lead-is-less-sensitive-than-pulse-pressure-variation-to-detect-changes-in-stroke-volume-after-fluid-challenge-in-icu-patients-postoperatively-to-cardiac-surgery
#4
COMPARATIVE STUDY
Christophe Soltner, Romain Dantec, Frédéric Lebreton, Julien Huntzinger, Laurent Beydon
OBJECTIVE: The amplitude of R-wave in DII lead (RDII) has been shown to correlate to central blood volume in animal and healthy volunteers. The aim of this study was to assess if change in RDII (DeltaRDII) after passive leg rise (PLR) and fluid loading would allow detecting preload dependence in intensive care ventilated patients. This parameter was compared to concomitant changes in pulse arterial pressure (DeltaPP). METHODS: Observational study in 40 stable sedated and ventilated cardiac surgery patients studied postoperatively...
April 2010: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/19488592/pulse-oximetry-wave-variation-as-a-noninvasive-tool-to-assess-volume-status-in-cardiac-surgery
#5
Glauco A Westphal, Eliezer Silva, Anderson Roman Gonçalves, Milton Caldeira Filho, Luíz F Poli-de-Figueiredo
OBJECTIVE: To compare variations of plethysmographic wave amplitude (DeltaPpleth) and to determine the percent difference between inspiratory and expiratory pulse pressure (DeltaPp) cutoff values for volume responsiveness in a homogenous population of postoperative cardiac surgery patients. INTRODUCTION: Intra-thoracic pressure variations interfere with stroke volume variation. Pulse pressure variations through arterial lines during mechanical ventilation have been recommended for the estimation of fluid responsiveness...
2009: Clinics
https://www.readbyqxmd.com/read/19347330/pulse-pressure-variations-adjusted-by-alveolar-driving-pressure-to-assess-fluid-responsiveness
#6
Fabrice Vallée, Jean Christophe M Richard, Arnaud Mari, Thomas Gallas, Eric Arsac, Pascale Sanchez Verlaan, Benjamin Chousterman, Kamran Samii, Michèle Genestal, Olivier Fourcade
OBJECTIVE: To evaluate the ability of DeltaPP/DeltaP [pulse pressure variations (DeltaPP) adjusted by alveolar pressure variations (DeltaP = Pplat-PEEPtot)] in predicting fluid responsiveness, to compare its accuracy to that of DeltaPP used alone and to evaluate the influence of tidal volume (Vt) on these two indices. DESIGN: Prospective study. SETTING: A 22-bed general intensive care unit (ICU). PATIENTS: Eighty-four surgical or medical ventilated patients requiring fluid challenge...
June 2009: Intensive Care Medicine
https://www.readbyqxmd.com/read/19151280/the-ability-of-stroke-volume-variations-obtained-with-vigileo-flotrac-system-to-monitor-fluid-responsiveness-in-mechanically-ventilated-patients
#7
Maxime Cannesson, Henri Musard, Olivier Desebbe, Cécile Boucau, Rémi Simon, Roland Hénaine, Jean-Jacques Lehot
BACKGROUND: Respiratory variations in arterial pulse pressure (DeltaPP) are accurate predictors of fluid responsiveness in mechanically ventilated patients. The aim of our study was to assess the ability of a novel algorithm for automatic estimation of stroke volume variation (SVV) to predict fluid responsiveness in mechanically ventilated patients. METHODS: We studied 25 patients referred for coronary artery bypass grafting. SVV was continuously displayed by the Vigileo/FloTrac system...
February 2009: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/18946297/poor-agreement-between-respiratory-variations-in-pulse-oximetry-photoplethysmographic-waveform-amplitude-and-pulse-pressure-in-intensive-care-unit-patients
#8
COMPARATIVE STUDY
Svein Aslak Landsverk, Lars O Hoiseth, Per Kvandal, Jonny Hisdal, Oivind Skare, Knut A Kirkeboen
BACKGROUND: To identify fluid responsiveness, a correlation between respiratory variations in pulse pressure (DeltaPP) and respiratory variations in pulse oximetry photoplethysmographic waveform amplitude (DeltaPOP) in mechanically ventilated patients has been demonstrated. To evaluate the agreement between the two methods, knowledge about the repeatability of the methods is imperative. However, no such data exist. Based on knowledge of slow oscillation in skin blood flow, the authors hypothesized that the variability of DeltaPOP would be larger than that of DeltaPP when calculations were performed continuously over a long recording period...
November 2008: Anesthesiology
https://www.readbyqxmd.com/read/18522935/pleth-variability-index-to-monitor-the-respiratory-variations-in-the-pulse-oximeter-plethysmographic-waveform-amplitude-and-predict-fluid-responsiveness-in-the-operating-theatre
#9
M Cannesson, O Desebbe, P Rosamel, B Delannoy, J Robin, O Bastien, J-J Lehot
BACKGROUND: Respiratory variations in pulse oximetry plethysmographic waveform amplitude (DeltaPOP) can predict fluid responsiveness in mechanically ventilated patients but cannot be easily assessed at the bedside. Pleth variability index (PVI) is a new algorithm allowing for automated and continuous monitoring of DeltaPOP. We hypothesized that PVI can predict fluid responsiveness in mechanically ventilated patients under general anaesthesia. METHODS: Twenty-five patients were studied after induction of general anaesthesia...
August 2008: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/18349193/online-monitoring-of-pulse-pressure-variation-to-guide-fluid-therapy-after-cardiac-surgery
#10
Jose Otavio Auler, Filomena Galas, Ludhmila Hajjar, Luciana Santos, Thiago Carvalho, Frédéric Michard
BACKGROUND: The arterial pulse pressure variation induced by mechanical ventilation (deltaPP) has been shown to be a predictor of fluid responsiveness. Until now, deltaPP has had to be calculated offline (from a computer recording or a paper printing of the arterial pressure curve), or to be derived from specific cardiac output monitors, limiting the widespread use of this parameter. Recently, a method has been developed for the automatic calculation and real-time monitoring of deltaPP using standard bedside monitors...
April 2008: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/18349192/the-ability-of-a-novel-algorithm-for-automatic-estimation-of-the-respiratory-variations-in-arterial-pulse-pressure-to-monitor-fluid-responsiveness-in-the-operating-room
#11
Maxime Cannesson, Juliette Slieker, Olivier Desebbe, Christian Bauer, Pascal Chiari, Roland Hénaine, Jean-Jacques Lehot
BACKGROUND: Respiratory variations in arterial pulse pressure (deltaPP(man)) are accurate predictors of fluid responsiveness in mechanically ventilated patients. However, they cannot be continuously monitored. In our study, we assessed the clinical utility of a novel algorithm for automatic estimation of deltaPP (deltaPP(auto)). METHODS: We studied 25 patients referred for coronary artery bypass grafting. DeltaPP(auto) was continuously displayed using a method based on automatic detection algorithms, kernel smoothing, and rank-order filters...
April 2008: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/18259726/respiratory-variations-in-aortic-blood-flow-predict-fluid-responsiveness-in-ventilated-children
#12
Philippe Durand, Laurent Chevret, Sandrine Essouri, Vincent Haas, Denis Devictor
OBJECTIVE: To investigate whether respiratory variations in aortic blood flow velocity (DeltaVpeak ao), systolic arterial pressure (DeltaPS) and pulse pressure (DeltaPP) could accurately predict fluid responsiveness in ventilated children. DESIGN AND SETTING: Prospective study in a 18-bed pediatric intensive care unit. PATIENTS: Twenty-six children [median age 28.5 (16-44) months] with preserved left ventricular (LV) function. INTERVENTION: Standardized volume expansion (VE)...
May 2008: Intensive Care Medicine
https://www.readbyqxmd.com/read/18164579/effects-of-the-knee-chest-position-on-cardiac-index-and-propofol-requirements-during-bispectral-index-bis-guided-spine-surgery
#13
L Bennarosh, C Peuch, J Cohen, C Dauzac, P Guigui, J Mantz, S Dahmani
INTRODUCTION: The knee-chest (KC) position is often used for spine surgery. It is considered to promote significant changes in venous return and cardiac output. However, the magnitude of these changes and their consequences on intraoperative haemodynamics and anaesthetic requirements remain to be determined. The goal of the present study was to determine the changes in cardiac index and propofol requirements of patients undergoing spine surgery in the KC position. METHODS: Twenty ASA 1-3 patients scheduled for elective spine surgery were included in the study...
February 2008: Annales Françaises D'anesthèsie et de Rèanimation
https://www.readbyqxmd.com/read/17939330/the-relationship-between-the-acute-changes-of-the-systolic-blood-pressure-and-the-brachial-ankle-pulse-wave-velocity
#14
COMPARATIVE STUDY
Hun-Jun Park, Tai-Ho Rho, Chan Seok Park, Sung Won Jang, Woo-Seung Shin, Yong-Seog Oh, Man-Young Lee, Eun-Ju Cho, Ki-Bae Seung, Jae-Hyung Kim, Kyu-Bo Choi
BACKGROUND: The brachial-ankle pulse wave velocity (baPWV) is a useful parameter to assess arterial stiffness. However, it is difficult to evaluate arterial stiffness in hypertensive patients because the baPWV is affected by the blood pressure itself. This study was designed to estimate the relationship between the change of the blood pressure parameters and the baPWV (deltabaPWV) when hypertensive patients were subjected to an acute reduction of blood pressure. METHODS: Thirty patients with essential hypertension and whose blood pressure was higher than 140/90 mmHg were enrolled...
September 2007: Korean Journal of Internal Medicine
https://www.readbyqxmd.com/read/17822565/goal-directed-fluid-management-based-on-pulse-pressure-variation-monitoring-during-high-risk-surgery-a-pilot-randomized-controlled-trial
#15
RANDOMIZED CONTROLLED TRIAL
Marcel R Lopes, Marcos A Oliveira, Vanessa Oliveira S Pereira, Ivaneide Paula B Lemos, Jose Otavio C Auler, Frédéric Michard
INTRODUCTION: Several studies have shown that maximizing stroke volume (or increasing it until a plateau is reached) by volume loading during high-risk surgery may improve post-operative outcome. This goal could be achieved simply by minimizing the variation in arterial pulse pressure (deltaPP) induced by mechanical ventilation. We tested this hypothesis in a prospective, randomized, single-centre study. The primary endpoint was the length of postoperative stay in hospital. METHODS: Thirty-three patients undergoing high-risk surgery were randomized either to a control group (group C, n = 16) or to an intervention group (group I, n = 17)...
2007: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/17714573/pulse-pressure-variation-as-a-tool-to-detect-hypovolaemia-during-pneumoperitoneum
#16
F Bliacheriene, S B Machado, E B Fonseca, D Otsuke, J O C Auler, F Michard
BACKGROUND: Pulse pressure variation (DeltaPP) and systolic pressure variation (SPV) induced by mechanical ventilation have been proposed to detect hypovolaemia and guide fluid therapy. During laparoscopic surgery, chest compliance is decreased by pneumoperitoneum. This may affect the value of SPV and DeltaPP as indicators of intravascular volume status. Thereby, we investigated the effects of pneumoperitoneum and hypovolaemia on SPV and DeltaPP. METHODS: We measured DeltaPP, SPV and the inspiratory (Deltaup) and expiratory (Deltadown) components of SPV, at baseline, during pneumoperitoneum, during pneumoperitoneum and hypovolaemia and after the return to baseline conditions, in 11 mechanically ventilated rabbits...
October 2007: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/17525584/respiratory-variations-in-pulse-oximetry-plethysmographic-waveform-amplitude-to-predict-fluid-responsiveness-in-the-operating-room
#17
Maxime Cannesson, Yassin Attof, Pascal Rosamel, Olivier Desebbe, Pierre Joseph, Olivier Metton, Olivier Bastien, Jean-Jacques Lehot
BACKGROUND: Respiratory variations in pulse oximetry plethysmographic waveform amplitude (DeltaPOP) are related to respiratory variations in pulse pressure (DeltaPP) and are sensitive to changes in preload. The authors hypothesized that DeltaPOP can predict fluid responsiveness in mechanically ventilated patients during general anesthesia. METHODS: Twenty-five patients referred for cardiac surgery were studied after induction of general anesthesia. Hemodynamic data (cardiac index, central venous pressure, pulmonary capillary wedge pressure, DeltaPP, and DeltaPOP) were recorded before and after volume expansion (500 ml hetastarch, 6%)...
June 2007: Anesthesiology
https://www.readbyqxmd.com/read/17508201/can-dynamic-indicators-help-the-prediction-of-fluid-responsiveness-in-spontaneously-breathing-critically-ill-patients
#18
Stéphane Soubrier, Fabienne Saulnier, Hervé Hubert, Pierre Delour, Hélène Lenci, Thierry Onimus, Saad Nseir, Alain Durocher
OBJECTIVE: To investigate whether the respiratory changes in arterial pulse (DeltaPP) and in systolic pressure (DeltaSP) could predict fluid responsiveness in spontaneously breathing (SB) patients. Because changes in intrathoracic pressure during spontaneous breathing (SB) might be insufficient to modify loading conditions of the ventricles, performances of indicators were also assessed during a forced respiratory maneuver. DESIGN: Prospective interventional study...
July 2007: Intensive Care Medicine
https://www.readbyqxmd.com/read/17494781/radial-artery-pulse-pressure-variation-correlates-with-brachial-artery-peak-velocity-variation-in-ventilated-subjects-when-measured-by-internal-medicine-residents-using-hand-carried-ultrasound-devices
#19
J Matthew Brennan, John E A Blair, Chetan Hampole, Sascha Goonewardena, Samip Vasaiwala, Dipak Shah, Kirk T Spencer, Gregory A Schmidt
BACKGROUND: Rapid prediction of the effect of volume expansion is crucial in unstable patients receiving mechanical ventilation. Both radial artery pulse pressure variation (DeltaPP) and change of aortic blood flow peak velocity are accurate predictors but may be impractical point-of-care tools. PURPOSES: We sought to determine whether respiratory changes in the brachial artery blood flow velocity (DeltaVpeak-BA) as measured by internal medicine residents using a hand-carried ultrasound (HCU) device could provide an accurate corollary to DeltaPP in patients receiving mechanical ventilation...
May 2007: Chest
https://www.readbyqxmd.com/read/17437497/pulse-pressure-respiratory-variation-as-an-early-marker-of-cardiac-output-fall-in-experimental-hemorrhagic-shock
#20
Glauco Westphal, Alejandra del Pilar Gallardo Garrido, Daniela Paoli de Almeida, Maurício Rocha e Silva, Luiz Francisco Poli-de-Figueiredo
Pulse pressure (DeltaPp) and systolic pressure (DeltaPs) variations have been recommended as predictors of fluid responsiveness in critically ill patients. We hypothesized that changes in DeltaPp and DeltaPs parallel alterations in stroke volume (SV) and cardiac output (CO) during hemorrhage, shock, and resuscitation. In anesthetized and mechanically ventilated mongrel dogs, a graded hemorrhage (20 mL/min) was induced to a target mean arterial pressure (MAP) of 40 mm Hg, which was maintained for additional 30 min...
April 2007: Artificial Organs
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