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https://www.readbyqxmd.com/read/28433392/quantifying-rates-of-cell-migration-and-cell-proliferation-in-co-culture-barrier-assays-reveals-how-skin-and-melanoma-cells-interact-during-melanoma-spreading-and-invasion
#1
Parvathi Haridas, Catherine J Penington, Jacqui A McGovern, D L Sean McElwain, Matthew J Simpson
Malignant spreading involves the migration of cancer cells amongst other native cell types. For example, in vivo melanoma invasion involves individual melanoma cells migrating through native skin, which is composed of several distinct subpopulations of cells. Here, we aim to quantify how interactions between melanoma and fibroblast cells affect the collective spreading of a heterogeneous population of these cells in vitro. We perform a suite of circular barrier assays that includes: (i) monoculture assays with fibroblast cells; (ii) monoculture assays with SK-MEL-28 melanoma cells; and (iii) a series of co-culture assays initiated with three different ratios of SK-MEL-28 melanoma cells and fibroblast cells...
April 19, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28432615/fabrication-physicochemical-characterization-and-performance-evaluation-of-biodegradable-polymeric-microneedle-patch-system-for-enhanced-transcutaneous-flux-of-high-molecular-weight-therapeutics
#2
Viral Shah, Bijaya Krushna Choudhury
A revolutionary paradigm shift is being observed currently, towards the use of therapeutic biologics for disease management. The present research was focused on designing an efficient dosage form for transdermal delivery of α-choriogonadotropin (high molecular weight biologic), through biodegradable polymeric microneedles. Polyvinylpyrrolidone-based biodegradable microneedle arrays loaded with high molecular weight polypeptide, α-choriogonadotropin, were fabricated for its systemic delivery via transdermal route...
April 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28431925/analysis-of-pm2-5-induced-cytotoxicity-in-human-hacat-cells-based-on-a-microfluidic-system
#3
Yuxiao Zhang, Lulu Zheng, Jiang Tuo, Qi Liu, Xinlian Zhang, Zhixuan Xu, Sixiu Liu, Guodong Sui
Human exposure to PM2.5 causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can has direct contact with PM2.5, but there is no study about PM2.5-induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of traditional research methods. In present study, we established a microfluidic system including a cell culture chip integrated with a high-throughput protein microarray chip to investigate the mechanism of PM2...
April 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28430185/phospholipase-c%C3%AE-1-regulates-p38-mapk-activity-and-skin-barrier-integrity
#4
Kaori Kanemaru, Yoshikazu Nakamura, Kengo Totoki, Takatsugu Fukuyama, Madoka Shoji, Hisae Kaneko, Kanako Shiratori, Atsuko Yoneda, Takafumi Inoue, Yoichiro Iwakura, Kenji Kabashima, Kiyoko Fukami
Keratinocytes undergo a unique type of programmed cell death known as cornification, which leads to the formation of the stratum corneum (SC), the main physical barrier of the epidermis. A defective epidermal barrier is a hallmark of the two most common inflammatory skin disorders, psoriasis, and atopic dermatitis. However, the detailed molecular mechanisms of skin barrier formation are not yet fully understood. Here, we showed that downregulation of phospholipase C (PLC) δ1, a Ca(2+)-mobilizing and phosphoinositide-metabolizing enzyme abundantly expressed in the epidermis, impairs the barrier functions of the SC...
April 21, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28427959/beneficial-effects-of-heparin-and-l-arginine-on-dermonecrosis-effect-induced-by-vipera-lebetina-venom-involvement-of-no-in-skin-regeneration
#5
Nouara Bennacef-Heffar, Fatima Laraba-Djebari
It is well known that snake venoms such as Viperidae caused severe local effects such as hemorrhage, myonecrosis and dermonecrosis which can lead to permanent tissue loss or the disability. The aim of this study is to evaluate the skin regeneration using heparin and L-arginine as well as the dermonecrotic effects induced by Vipera lebetina venom (VLV). To better understand the toxic effects induced by VLV and to prevent or treat these effects, we evaluate the local effects and the skin regeneration with or without drugs...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28426265/transdermal-iontophoresis-patch-with-reverse-electrodialysis
#6
Joon Lee, Kilsung Kwon, Minyoung Kim, Joonhong Min, Nathaniel S Hwang, Won-Serk Kim
Reverse electrodialysis (RED) technology generates energy from the salinity gradient by contacting waters with different salinity. Herein, we develop the disposable skin patch using this eco-friendly energy. The current density, which can be controlled easily without special circuit, is enough to iontophoretic drug delivery. In vitro study, this iontophoretic system enhanced the transdermal delivery of peptide, which is difficult to penetrate the skin barrier by simple diffusion. We design the disposable iontophoretic skin patch using RED system and suggest this patch can be apply on new cosmetic patch or disposable drug patch...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28425595/hand-eczema-atopic-dermatitis-and-filaggrin-mutations-in-adult-danes-a-registry-based-study-assessing-risk-of-disability-pension
#7
Nina G Heede, Betina H Thuesen, Jacob P Thyssen, Allan Linneberg, Pal B Szecsi, Steen Stender, Torkil Menné, Jeanne D Johansen
BACKGROUND: Atopic dermatitis and hand eczema often impair the ability of people to work. Only a few studies have investigated whether individuals with loss-of-function filaggrin gene (FLG) mutations, who often have severe and early onset of dermatitis, experience occupational consequences. OBJECTIVE: To investigate the personal consequences of having atopic dermatitis and/or hand eczema and FLG mutations. METHOD: Adult Danes from the general population (n = 3247) and patients with atopic dermatitis and/or hand eczema (n = 496) were genotyped for common FLG mutations, and completed a questionnaire about skin symptoms and hand eczema...
April 20, 2017: Contact Dermatitis
https://www.readbyqxmd.com/read/28424689/antimicrobial-and-attractant-roles-for-chemerin-in-the-oral-cavity-during-inflammatory-gum-disease
#8
Urszula Godlewska, Piotr Brzoza, Aneta Sroka, Pawel Majewski, Holger Jentsch, Martin Eckert, Sigrun Eick, Jan Potempa, Brian A Zabel, Joanna Cichy
Periodontal inflammation is one of the most common chronic inflammatory conditions in humans. Despite recent advances in identifying and characterizing oral microbiota dysbiosis in the pathogenesis of gum disease, just how host factors maintain a healthy homeostatic oral microbial community or prevent the development of a pathogenic oral microbiota remains poorly understood. An important determinant of microbiota fate is local antimicrobial proteins. Here, we report that chemoattractant protein chemerin, which we recently identified as a potent endogenous antimicrobial agent in body barriers such as the skin, is present in the oral cavity under homeostatic and inflammatory conditions...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28423680/a-bioartificial-dermal-regeneration-template-promotes-skin-cell-proliferation-in-vitro-and-enhances-large-skin-wound-healing-in-vivo
#9
Peng Chang, Bingyu Guo, Qiang Hui, Xiaoyan Liu, Kai Tao
A novel bioartificial dermal regeneration template has been developed using platelet-rich plasma and acellular animal skin collagen sponge for the treatment of larger area and full thickness skin wounds. This platelet-rich plasma-collagen sponge keeps native skin structure and contains huge amounts of growth factors. The effect of this bioartificial dermal regeneration template was tested in vitro and in vivo via a mimic poor wound healing process by adding collagenase I into cell culture medium or the wound area...
March 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422481/gold-nanoparticles-induced-endothelial-leakiness-depends-on-particle-size-and-endothelial-cell-origin
#10
Magdiel I Setyawati, Chor Yong Tay, Boon Huat Bay, David T Leong
The endothelium presents a formidable barrier for cancer nanomedicine, as the intravenously introduced nanomedicine needs to leave the blood vessel at the tumor site. Endothelial permeability and retention effect (EPR) is not dependable since it is derived from tumors. Certain nanoparticles with specific characteristics are able to induce micrometer sized gaps between endothelial cells. This effect is called "nanoparticle induced endothelial leakiness" (NanoEL). NanoEL therefore allows the nanotechnology to control access to the tumor even in the absence of any EPR effect...
April 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28420063/patients-experiences-of-lupus-related-foot-problems-a-qualitative-investigation
#11
A E Williams, A Blake, L Cherry, B Alcacer-Pitarch, C J Edwards, N Hopkinson, E M J Vital, L S Teh
Background Systemic lupus erythematosus (SLE) can present with a variety of symptoms. Previous research has shown there is a high prevalence of lower limb and foot problems in patients with SLE associated with the musculoskeletal, vascular and neurological changes. Furthermore, there is a high prevalence of infections affecting the feet and a range of common skin and nail problems. However, it is not known how these foot problems impact upon people's lives. Therefore, we aimed to explore this using a qualitative approach...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28419984/topical-histamine-stimulates-repigmentation-of-nonsegmental-vitiligo-by-a-receptor-dependent-mechanism
#12
Jun Liu, Yan Xu, Tzu-Kai Lin, Chengzhi Lv, Peter M Elias, Mao-Qiang Man
BACKGROUND: Though vitiligo is a common depigmentary disorder, it still represents a substantial therapeutic challenge. Therapeutic options are limited in part due to its uncertain etiology. OBJECTIVE: Because recent studies suggest that histamine stimulates melanogenesis in vitro, we determined here whether topical histamine stimulates repigmentation in patients with stable, nonsegmental vitiligo. METHODS: A total of 23 otherwise normal volunteers with vitiligo, including 14 males and 9 females aged 6-59 years (mean age 29...
April 19, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28418129/ichthyoses-new-insights-unravel-defects-in-skin-barrier-function-resulting-in-diverse-clinical-and-morphological-phenotypes
#13
I Hausser
No abstract text is available yet for this article.
April 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28414878/do-outdoor-workers-know-their-risk-of-nmsc-perceptions-beliefs-and-preventive-behaviour-among-farmers-roofers-and-gardeners
#14
A Zink, D Wurstbauer, M Rotter, M Wildner, T Biedermann
BACKGROUND: Non-melanoma skin cancer (NMSC) was officially recognized in 2015 as an occupational disease for outdoor workers in Germany. Together with the enormous socioeconomic impact of NMSC, this has led to the continuous demand of evidence-based prevention. However, studies assessing the perceptions and beliefs along with risk behavior of outdoor workers as an essential prerequisite for prevention are rare. OBJECTIVE: To assess perceptions, beliefs, barriers, risk and preventive behavior towards non-melanoma skin cancer among different outdoor groups as a basis for the development of sustainable prevention programs...
April 17, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28413108/moisturisers-in-scar-management-following-burn-a-survey-report
#15
Tanja Klotz, Rochelle Kurmis, Zachary Munn, Kathryn Heath, John Greenwood
Scar management is a recognised key component of rehabilitation following burn. Moisturising often combined with massage is commenced once healing tissue has gained sufficient strength to tolerate surface friction, with the aim being to hydrate the dry scar. The studies on various moisturisers and creams provide some guidance on moisturiser selection, but many are inconclusive. OBJECTIVE: This survey aimed to determine the current expert opinion regarding moisturiser recommendations, including the basis for these recommendations, across the burns community...
April 13, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28412298/tight-junction-proteins-claudin-1-and-occludin-are-important-for-cutaneous-wound-healing
#16
Thomas Volksdorf, Janina Heilmann, Sabine A Eming, Kathrin Schawjinski, Michaela Zorn-Kruppa, Christopher Ueck, Sabine Vidal-Y-Sy, Sabine Windhorst, Manfred Jücker, Ingrid Moll, Johanna M Brandner
Tight junction (TJ) proteins are known to be involved in proliferation and differentiation. These processes are essential for normal skin wound healing. Here, we investigated the TJ proteins claudin-1 and occludin in ex vivo skin wound healing models and tissue samples of acute and chronic human wounds and observed major differences in localization/expression of these proteins, with chronic wounds often showing a loss of the proteins at the wound margins and/or in the regenerating epidermis. Knockdown experiments in primary human keratinocytes showed that decreased claudin-1 expression resulted in significantly impaired scratch wound healing, with delayed migration and reduced proliferation...
April 12, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28411854/the-molecular-revolution-in-cutaneous-biology-chromosomal-territories-higher-order-chromatin-remodeling-and-the-control-of%C3%A2-gene-expression-in-keratinocytes
#17
REVIEW
Vladimir A Botchkarev
Three-dimensional organization of transcription in the nucleus and mechanisms controlling the global chromatin folding, including spatial interactions between the genes, noncoding genome elements, and epigenetic and transcription machinery, are essential for establishing lineage-specific gene expression programs during cell differentiation. Spatial chromatin interactions in the nucleus involving gene promoters and distal regulatory elements are currently considered major forces that drive cell differentiation and genome evolution in general, and such interactions are substantially reorganized during many pathological conditions...
May 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28411834/glucocorticoids-inhibit-wound-healing-novel-mechanism-of-action
#18
Andrzej T Slominski, Michal A Zmijewski
Jozic et al. describe mechanisms of glucocorticoid (GC) downregulation of wound healing by interaction with the membrane bound GC receptor, followed by stimulation of β-catenin and c-myc pathways. Targeting the membrane bound GC receptor or the recently discovered interaction of GC with mineralocorticoid receptors may counteract negative effects of GC on the skin barrier and potentially could serve as a remedy for age-related skin atrophy.
May 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28410606/in-vivo-quantification-of-quantum-dot-systemic-transport-in-c57bl-6-hairless-mice-following-skin-application-post-ultraviolet-radiation
#19
Samreen Jatana, Brian C Palmer, Sarah J Phelan, Robert Gelein, Lisa A DeLouise
BACKGROUND: Previous work has demonstrated size, surface charge and skin barrier dependent penetration of nanoparticles into the viable layers of mouse skin. The goal of this work was to characterize the tissue distribution and mechanism of transport of nanoparticles beyond skin, with and without Ultraviolet Radiation (UVR) induced skin barrier disruption. Atomic absorption spectroscopy (AAS), flow cytometry and confocal microscopy were used to examine the effect of UVR dose (180 and 360 mJ/cm(2) UVB) on the skin penetration and systemic distribution of quantum dot (QD) nanoparticles topically applied at different time-points post UVR using a hairless C57BL/6 mouse model...
April 14, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28410558/ultrasensitive-vertical-piezotronic-transistor-based-on-zno-twin-nanoplatelet
#20
Longfei Wang, Shuhai Liu, Xiaolong Feng, Qi Xu, Suo Bai, Laipan Zhu, Libo Chen, Yong Qin, Zhong Lin Wang
High sensitivity of pressure/strain sensor is the key to accurately evaluate external mechanical stimuli and could become more important in future generations of human-machine interfaces and artificial skin. Here we report the study of a two-terminal piezotronic transistor based on ZnO twin nanoplatelets (TNPT). Owing to the mirror symmetrical structure of ZnO twin nanplatelet, compressive pressure induced positive piezoelectric polarization charges created at both metal-semiconductor interfaces can simultaneously lower the both Schottky barrier heights and thus significantly modulate the carriers transport...
April 14, 2017: ACS Nano
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