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HIRA chaperone

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https://www.readbyqxmd.com/read/27871933/a-molecular-prospective-for-hira-complex-assembly-and-h3-3-specific-histone-chaperone-function
#1
REVIEW
M Daniel Ricketts, Ronen Marmorstein
Incorporation of variant histone sequences, in addition to post-translational modification of histones, serves to modulate the chromatin environment. Different histone chaperone proteins mediate the storage and chromatin deposition of variant histones. Although the two non-centromeric histone H3 variants, H3.1 and H3.3, differ by only 5 aa, replacement of histone H3.1 with H3.3 can modulate the transcription for highly expressed and developmentally required genes, lead to the formation of repressive heterochromatin, or aid in DNA and chromatin repair...
November 19, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27518902/hira-is-required-for-heart-development-and-directly-regulates-tnni2-and-tnnt3
#2
Daniel Dilg, Rasha Noureldin M Saleh, Sarah Elizabeth Lee Phelps, Yoann Rose, Laurent Dupays, Cian Murphy, Timothy Mohun, Robert H Anderson, Peter J Scambler, Ariane L A Chapgier
Chromatin remodelling is essential for cardiac development. Interestingly, the role of histone chaperones has not been investigated in this regard. HIRA is a member of the HUCA (HIRA/UBN1/CABIN1/ASF1a) complex that deposits the variant histone H3.3 on chromatin independently of replication. Lack of HIRA has general effects on chromatin and gene expression dynamics in embryonic stem cells and mouse oocytes. Here we describe the conditional ablation of Hira in the cardiogenic mesoderm of mice. We observed surface oedema, ventricular and atrial septal defects and embryonic lethality...
2016: PloS One
https://www.readbyqxmd.com/read/27515126/differential-regulation-of-the-histone-chaperone-hira-during-muscle-cell-differentiation-by-a-phosphorylation-switch
#3
Jae-Hyun Yang, Tae-Yang Song, Chanhee Jo, Jinyoung Park, Han-Young Lee, Ilang Song, Suji Hong, Kwan Young Jung, Jaehoon Kim, Jeung-Whan Han, Hong-Duk Youn, Eun-Jung Cho
Replication-independent incorporation of variant histone H3.3 has a profound impact on chromatin function and numerous cellular processes, including the differentiation of muscle cells. The histone chaperone HIRA and H3.3 have essential roles in MyoD regulation during myoblast differentiation. However, the precise mechanism that determines the onset of H3.3 deposition in response to differentiation signals is unclear. Here we show that HIRA is phosphorylated by Akt kinase, an important signaling modulator in muscle cells...
2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27423868/chip-sequencing-to-map-the-epigenome-of-senescent-cells-using-benzonase-endonuclease
#4
T S Rai, P D Adams
Cellular senescence is a state of stable cell cycle arrest triggered by diverse stresses. Establishment of senescence occurs in conjunction with a multitude of chromatin changes, which are just beginning to be studied. These chromatin changes are hypothesized to be causative for senescence. Currently, a preferred method to study such changes is chromatin immunoprecipitation followed by sequencing (ChIP-Seq). This is usually done by cross-linking the cells with formaldehyde and then generating chromatin fragments between 150 and 300bp by sonication...
2016: Methods in Enzymology
https://www.readbyqxmd.com/read/27372750/functional-characterization-of-histone-chaperones-using-snap-tag-based-imaging-to-assess-de-novo-histone-deposition
#5
C Clément, I Vassias, D Ray-Gallet, G Almouzni
Histone chaperones-key actors in the dynamic organization of chromatin-interact with the various histone variants to ensure their transfer in and out of chromatin. In vitro chromatin assembly assays and isolation of protein complexes using tagged histone variants provided first clues concerning their binding specificities and mode of action. Here, we describe an in vivo method using SNAP-tag-based imaging to assess the de novo deposition of histones and the role of histone chaperones. This method exploits cells expressing SNAP-tagged histones combined with individual cell imaging to visualize directly de novo histone deposition in vivo...
2016: Methods in Enzymology
https://www.readbyqxmd.com/read/27343236/restriction-of-retrotransposon-mobilization-in-schizosaccharomyces-pombe-by-transcriptional-silencing-and-higher-order-chromatin-organization
#6
Heather E Murton, Patrick J R Grady, Tsun Ho Chan, Hugh P Cam, Simon K Whitehall
Uncontrolled propagation of retrotransposons is potentially detrimental to host genome integrity. Therefore, cells have evolved surveillance mechanisms to restrict the mobility of these elements. In Schizosaccharomyces pombe the Tf2 LTR retrotransposons are transcriptionally silenced and are also clustered in the nucleus into structures termed Tf bodies. Here we describe the impact of silencing and clustering on the mobility of an endogenous Tf2 element. Deletion of genes such as set1(+) (histone H3 lysine 4 methyltransferase) or abp1(+) (CENP-B homolog) that both alleviate silencing and clustering, result in a corresponding increase in mobilization...
August 2016: Genetics
https://www.readbyqxmd.com/read/27269284/nucleosome-disassembly-during-human-non-homologous-end-joining-followed-by-concerted-hira-and-caf-1-dependent-reassembly
#7
Xuan Li, Jessica K Tyler
The cell achieves DNA double-strand break (DSB) repair in the context of chromatin structure. However, the mechanisms used to expose DSBs to the repair machinery and to restore the chromatin organization after repair remain elusive. Here we show that induction of a DSB in human cells causes local nucleosome disassembly, apparently independently from DNA end resection. This efficient removal of histone H3 from the genome during non-homologous end joining was promoted by both ATM and the ATP-dependent nucleosome remodeler INO80...
2016: ELife
https://www.readbyqxmd.com/read/27217568/o-linked-n-acetylglucosamine-transferase-ogt-interacts-with-the-histone-chaperone-hira-complex-and-regulates-nucleosome-assembly-and-cellular-senescence
#8
Jong-Sun Lee, Zhiguo Zhang
The histone chaperone HIRA complex, consisting of histone cell cycle regulator (HIRA), Ubinuclein1 (UBN1), and calcineurin binding protein 1 (CABIN1), deposits histone variant H3.3 to genic regions and regulates gene expression in various cellular processes, including cellular senescence. How HIRA-mediated nucleosome assembly of H3.3-H4 is regulated remains not well understood. Here, we show that O-linked N-acetylglucosamine (GlcNAc) transferase (OGT), an enzyme that catalyzes O-GlcNAcylation of serine or threonine residues, interacts with UBN1, modifies HIRA, and promotes nucleosome assembly of H3...
June 7, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26935106/cardiomyocyte-specific-conditional-knockout-of-the-histone-chaperone-hira-in-mice-results-in-hypertrophy-sarcolemmal-damage-and-focal-replacement-fibrosis
#9
Nicolas Valenzuela, Qiying Fan, Faisal Fa'ak, Benjamin Soibam, Harika Nagandla, Yu Liu, Robert J Schwartz, Bradley K McConnell, M David Stewart
HIRA is the histone chaperone responsible for replication-independent incorporation of histone variant H3.3 within gene bodies and regulatory regions of actively transcribed genes, and within the bivalent promoter regions of developmentally regulated genes. The HIRA gene lies within the 22q11.2 deletion syndrome critical region; individuals with this syndrome have multiple congenital heart defects. Because terminally differentiated cardiomyocytes have exited the cell cycle, histone variants should be utilized for the bulk of chromatin remodeling...
March 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/26926299/maintenance-of-tissue-pluripotency-by-epigenetic-factors-acting-at-multiple-levels
#10
Devendran A Sadasivam, Der-Hwa Huang
Pluripotent stem cells often adopt a unique developmental program while retaining certain flexibility. The molecular basis of such properties remains unclear. Using differentiation of pluripotent Drosophila imaginal tissues as assays, we examined the contribution of epigenetic factors in ectopic activation of Hox genes. We found that over-expression of Trithorax H3K4 methyltransferase can induce ectopic adult appendages by selectively activating the Hox genes Ultrabithorax and Sex comb reduced in wing and leg discs, respectively...
February 2016: PLoS Genetics
https://www.readbyqxmd.com/read/26596463/live-detection-and-purification-of-cells-based-on-the-expression-of-a-histone-chaperone-hira-using-a-binding-peptide
#11
K J Kochurani, Annie A Suganya, Madhumathy G Nair, Jiss Maria Louis, Aditi Majumder, Santhosh K Kumar, Parvin Abraham, Debasree Dutta, Tessy T Maliekal
Flow cytometry is a reliable method for identification and purification of live cells from a heterogeneous population. Since permeabilized cells cannot be sorted live in a FACS sorter, its application in isolation of functional cells largely depends on antibodies for surface markers. In various fields of biology we find intracellular markers that reveal subpopulations of biological significance. Cell cycle stage specific molecules, metastatic signature molecules, stemness associated proteins etc. are examples of potential markers that could improve the research and therapy enormously...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26549683/continuous-histone-replacement-by-hira-is-essential-for-normal-transcriptional-regulation-and-de-novo-dna-methylation-during-mouse-oogenesis
#12
Buhe Nashun, Peter W S Hill, Sebastien A Smallwood, Gopuraja Dharmalingam, Rachel Amouroux, Stephen J Clark, Vineet Sharma, Elodie Ndjetehe, Pawel Pelczar, Richard J Festenstein, Gavin Kelsey, Petra Hajkova
The integrity of chromatin, which provides a dynamic template for all DNA-related processes in eukaryotes, is maintained through replication-dependent and -independent assembly pathways. To address the role of histone deposition in the absence of DNA replication, we deleted the H3.3 chaperone Hira in developing mouse oocytes. We show that chromatin of non-replicative developing oocytes is dynamic and that lack of continuous H3.3/H4 deposition alters chromatin structure, resulting in increased DNase I sensitivity, the accumulation of DNA damage, and a severe fertility phenotype...
November 19, 2015: Molecular Cell
https://www.readbyqxmd.com/read/26159857/ubinuclein-1-confers-histone-h3-3-specific-binding-by-the-hira-histone-chaperone-complex
#13
M Daniel Ricketts, Brian Frederick, Henry Hoff, Yong Tang, David C Schultz, Taranjit Singh Rai, Maria Grazia Vizioli, Peter D Adams, Ronen Marmorstein
Histone chaperones bind specific histones to mediate their storage, eviction or deposition from/or into chromatin. The HIRA histone chaperone complex, composed of HIRA, ubinuclein-1 (UBN1) and CABIN1, cooperates with the histone chaperone ASF1a to mediate H3.3-specific binding and chromatin deposition. Here we demonstrate that the conserved UBN1 Hpc2-related domain (HRD) is a novel H3.3-specific-binding domain. Biochemical and biophysical studies show the UBN1-HRD preferentially binds H3.3/H4 over H3.1/H4. X-ray crystallographic and mutational studies reveal that conserved residues within the UBN1-HRD and H3...
2015: Nature Communications
https://www.readbyqxmd.com/read/25849155/histone-chaperones-in-arabidopsis-and-rice-genome-wide-identification-phylogeny-architecture-and-transcriptional-regulation
#14
Amit K Tripathi, Khushwant Singh, Ashwani Pareek, Sneh L Singla-Pareek
BACKGROUND: Histone chaperones modulate chromatin architecture and hence play a pivotal role in epigenetic regulation of gene expression. In contrast to their animal and yeast counterparts, not much is known about plant histone chaperones. To gain insights into their functions in plants, we sought to identify histone chaperones from two model plant species and investigated their phylogeny, domain architecture and transcriptional profiles to establish correlation between their expression patterns and potential role in stress physiology and plant development...
2015: BMC Plant Biology
https://www.readbyqxmd.com/read/25847244/histone-chaperone-hira-in-regulation-of-transcription-factor-runx1
#15
Aditi Majumder, Khaja Mohieddin Syed, Sunu Joseph, Peter J Scambler, Debasree Dutta
RUNX1 (Runt-related transcription factor 1) is indispensable for the generation of hemogenic endothelium. However, the regulation of RUNX1 during this developmental process is poorly understood. We investigated the role of the histone chaperone HIRA (histone cell cycle regulation-defective homolog A) from this perspective and report that HIRA significantly contributes toward the regulation of RUNX1 in the transition of differentiating mouse embryonic stem cells from hemogenic to hematopoietic stage. Direct interaction of HIRA and RUNX1 activates the downstream targets of RUNX1 implicated in generation of hematopoietic stem cells...
May 22, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25666827/intellectual-disability-associated-dbrwd3-regulates-gene-expression-through-inhibition-of-hira-yem-mediated-chromatin-deposition-of-histone-h3-3
#16
Wei-Yu Chen, Hsueh-Tzu Shih, Kwei-Yan Liu, Kuei-Yan Liu, Zong-Siou Shih, Li-Kai Chen, Tsung-Han Tsai, Mei-Ju Chen, Hsuan Liu, Bertrand Chin-Ming Tan, Chien-Yu Chen, Hsiu-Hsiang Lee, Benjamin Loppin, Ounissa Aït-Ahmed, June-Tai Wu
Many causal mutations of intellectual disability have been found in genes involved in epigenetic regulations. Replication-independent deposition of the histone H3.3 variant by the HIRA complex is a prominent nucleosome replacement mechanism affecting gene transcription, especially in postmitotic neurons. However, how HIRA-mediated H3.3 deposition is regulated in these cells remains unclear. Here, we report that dBRWD3, the Drosophila ortholog of the intellectual disability gene BRWD3, regulates gene expression through H3...
April 2015: EMBO Reports
https://www.readbyqxmd.com/read/25602522/the-impact-of-the-hira-histone-chaperone-upon-global-nucleosome-architecture
#17
Csenge Gal, Karen M Moore, Konrad Paszkiewicz, Nicholas A Kent, Simon K Whitehall
HIRA is an evolutionarily conserved histone chaperone that mediates replication-independent nucleosome assembly and is important for a variety of processes such as cell cycle progression, development, and senescence. Here we have used a chromatin sequencing approach to determine the genome-wide contribution of HIRA to nucleosome organization in Schizosaccharomyces pombe. Cells lacking HIRA experience a global reduction in nucleosome occupancy at gene sequences, consistent with the proposed role for HIRA in chromatin reassembly behind elongating RNA polymerase II...
2015: Cell Cycle
https://www.readbyqxmd.com/read/25600486/the-histone-chaperone-complex-hir-maintains-nucleosome-occupancy-and-counterbalances-impaired-histone-deposition-in-caf-1-complex-mutants
#18
Céline Duc, Matthias Benoit, Samuel Le Goff, Lauriane Simon, Axel Poulet, Sylviane Cotterell, Christophe Tatout, Aline V Probst
Chromatin organization is essential for coordinated gene expression, genome stability, and inheritance of epigenetic information. The main components involved in chromatin assembly are specific complexes such as Chromatin Assembly Factor 1 (CAF-1) and Histone Regulator (HIR), which deposit histones in a DNA synthesis-dependent or -independent manner, respectively. Here, we characterize the role of the plant orthologs Histone Regulator A (HIRA), Ubinuclein (UBN) and Calcineurin Binding protein 1 (CABIN1), which constitute the HIR complex...
March 2015: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/25512559/hira-orchestrates-a-dynamic-chromatin-landscape-in-senescence-and-is-required-for-suppression-of-neoplasia
#19
Taranjit Singh Rai, John J Cole, David M Nelson, Dina Dikovskaya, William J Faller, Maria Grazia Vizioli, Rachael N Hewitt, Orchi Anannya, Tony McBryan, Indrani Manoharan, John van Tuyn, Nicholas Morrice, Nikolay A Pchelintsev, Andre Ivanov, Claire Brock, Mark E Drotar, Colin Nixon, William Clark, Owen J Sansom, Kurt I Anderson, Ayala King, Karen Blyth, Peter D Adams
Cellular senescence is a stable proliferation arrest that suppresses tumorigenesis. Cellular senescence and associated tumor suppression depend on control of chromatin. Histone chaperone HIRA deposits variant histone H3.3 and histone H4 into chromatin in a DNA replication-independent manner. Appropriately for a DNA replication-independent chaperone, HIRA is involved in control of chromatin in nonproliferating senescent cells, although its role is poorly defined. Here, we show that nonproliferating senescent cells express and incorporate histone H3...
December 15, 2014: Genes & Development
https://www.readbyqxmd.com/read/25197097/transcription-factor-eklf-klf1-recruitment-of-the-histone-chaperone-hira-is-essential-for-%C3%AE-globin-gene-expression
#20
Shefali Soni, Nikolay Pchelintsev, Peter D Adams, James J Bieker
The binding of chromatin-associated proteins and incorporation of histone variants correlates with alterations in gene expression. These changes have been particularly well analyzed at the mammalian β-globin locus, where transcription factors such as erythroid Krüppel-like factor (EKLF), which is also known as Krüppel-like factor 1 (KLF1), play a coordinating role in establishing the proper chromatin structure and inducing high-level expression of adult β-globin. We had previously shown that EKLF preferentially interacts with histone H3 and that the H3...
September 16, 2014: Proceedings of the National Academy of Sciences of the United States of America
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