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https://www.readbyqxmd.com/read/29510164/an-ns1abp-like-gene-promotes-white-spot-syndrome-virus-infection-by-interacting-with-the-viral-envelope-protein-vp28-in-red-claw-crayfish-cherax-quadricarinatus
#1
Xiao-Lu Xie, Xue-Jiao Chang, Yan Gao, Dong-Li Li, Ling-Ke Liu, Man-Jun Liu, Ke-Jian Wang, Hai-Peng Liu
Influenza A virus non-structural-1A binding protein (named as Ns1abp) was originally identified as a host protein from human that bound to the viral NS-1 protein. In our previous study, the expression of an Ns1abp-like gene (denoted as CqNs1abp-like gene) was found to be up-regulated in a transcriptome library from the haematopoietic tissue (Hpt) cells of red claw crayfish Cherax quadricarinatus post white spot syndrome virus (WSSV) infection. To elucidate the role of CqNs1abp-like gene involved in WSSV infection, we cloned the CqNs1abp-like gene in which the open reading frame was 2232 bp, encoding 743 amino acids with two typical domains of one BTB (Broad-Complex, Tramtrack and Bric a brac) domain at N-terminal and six Kelch domains at C-terminal...
March 3, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29428488/a-thymosin-repeated-protein1-reduces-white-spot-syndrome-virus-replication-in-red-claw-crayfish-cherax-quadricarinatus
#2
Dong-Li Li, Xue-Jiao Chang, Xiao-Lu Xie, Shu-Cheng Zheng, Qiu-Xia Zhang, Shu-Ao Jia, Ke-Jian Wang, Hai-Peng Liu
The β-thymosins are a group of structurally related, highly conserved intracellular small peptides in vertebrates with various biological functions, including cytoskeletal remodeling, neuronal development, cell migration, cell survival, tissue repair and inhibition of inflammation. In contrast to vertebrates, the function of β-thymosin is not fully understood in crustaceans. Previously, we found that a thymosin-repeated protein1 (CqTRP1) gene was up-regulated after white spot syndrome virus (WSSV) challenge in haematopoietic tissue (Hpt) cells from the red claw crayfish Cherax quadricarinatus...
February 8, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29341872/a-cqferritin-protein-inhibits-white-spot-syndrome-virus-infection-via-regulating-iron-ions-in-red-claw-crayfish-cherax-quadricarinatus
#3
Xiao-Xiao Chen, Yan-Yao Li, Xue-Jiao Chang, Xiao-Lu Xie, Yu-Ting Liang, Ke-Jian Wang, Wen-Yun Zheng, Hai-Peng Liu
It is well known that iron is an essential element for all living organism. The intracellular iron availability is also important for the host's innate immune response to various pathogens, in which the iron homeostasis can be regulated by ferritin due to its iron storage property. In this study, a full-length cDNA sequence of ferritin (named as CqFerritin) was identified with 1410 bp from red claw crayfish Cherax quadricarinatus, which contained an open reading frame of 513 bp, encoding 170 amino acids with a conserved ferritin domain...
January 13, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29102705/a-laminin-receptor-like-protein-regulates-white-spot-syndrome-virus-infection-by-binding-to-the-viral-envelope-protein-vp28-in-red-claw-crayfish-cherax-quadricarinatus
#4
Ling-Ke Liu, Wei-Dong Li, Yan Gao, Rong-Yuan Chen, Xiao-Lu Xie, Heng Hong, Ke-Jian Wang, Hai-Peng Liu
White spot syndrome virus (WSSV) is a lethal pathogen of shrimp and many other crustaceans, which has been causing huge economic losses in global aquaculture. Laminin receptor (LR) is a cell surface receptor which participates in the interactions between cells as well as cells and extracellular matrix. Previously, we found that a CqLR-like gene was responsive to WSSV infection in the hematopoietic tissue (Hpt) cells from red claw crayfish Cherax quadricarinatus. To further reveal the role of CqLR-like gene involved in WSSV infection, the full-length cDNA of CqLR-like gene was cloned with 1000 bp, and the open reading frame encoded 308 amino acids with a conserved laminin-binding domain...
November 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28805145/pdgf-vegf-related-receptor-affects-transglutaminase-activity-to-control-cell-migration-during-crustacean-hematopoiesis
#5
Kingkamon Junkunlo, Kenneth Söderhäll, Chadanat Noonin, Irene Söderhäll
The platelet-derived growth factor (PDGF) receptor, a tyrosine kinase (TK) receptor whose ligand is PDGF, is crucial in the transduction of extracellular signals into cells and mediates numerous processes, such as cell proliferation, differentiation, survival, and migration. We demonstrate the important roles of a receptor TK related to the PDGF/VEGF family protein (PVR) in controlling hematopoietic progenitor cell migration by affecting extracellular transglutaminase (TGase) activity. Pl_PVR1, GenBank accession No...
October 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28705723/a-pacifastacus-leniusculus-serine-protease-interacts-with-wssv
#6
Enen Guo, Gül Gizem Korkut, Phattarunda Jaree, Irene Söderhäll, Kenneth Söderhäll
Serine proteases are involved in many critical physiological processes including virus spread and replication. In the present study, we identified a new clip-domain serine protease (PlcSP) in the crayfish Pacifastacus leniusculus hemocytes, which can interact with the White Spot Syndrome Virus (WSSV) envelope protein VP28. It was characterized by a classic clip domain with six strictly conserved Cys residues, and contained the conserved His-Asp-Ser (H-D-S) motif in the catalytic domain. Furthermore, signal peptide prediction revealed that it has a 16-residue secretion signal peptide...
July 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28528959/role-of-astakine1-in-regulating-transglutaminase-activity
#7
Ratchanok Sirikharin, Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll
Transglutaminase (TGase) has been implicated in maintaining the undifferentiated stage of hematopoietic stem cells (HSC) in the crayfish Pacifastacus leniusculus. TGase activity has been reported to be regulated by astakine1, an essential crayfish cytokine for inducing new hemocyte synthesis in hematopoietic tissue (HPT). Here, the role of astakine1 in TGase activity regulation and clotting protein (CP) cross-linking was characterized. A reduction in TGase activity was observed by the addition of purified astakine1 in vitro for both endogenous crayfish TGase and a commercial purified guinea pig liver TGase...
May 19, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28479346/cqtoll-participates-in-antiviral-response-against-white-spot-syndrome-virus-via-induction-of-anti-lipopolysaccharide-factor-in-red-claw-crayfish-cherax-quadricarinatus
#8
Yan-Yao Li, Xiao-Xiao Chen, Feng-Yu Lin, Qiu-Fan Chen, Xing-Yuan Ma, Hai-Peng Liu
It is well known that Tolls/Toll like receptors (TLRs), a family of pattern recognition receptors, play important roles in immune responses. Previously, we found that a Toll transcript was increased in a transcriptome library of haematopoietic tissue (Hpt) cells from the red claw crayfish Cherax quadricarinatus post white spot syndrome virus infection. In the present study, a full-length cDNA sequence of Toll receptor (named as CqToll) was identified with 3482 bp which contained an open reading frame of 3021 bp encoding 1006 amino acids...
May 4, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27825947/characterization-of-micrornas-by-deep-sequencing-in-red-claw-crayfish-cherax-quadricarinatus-haematopoietic-tissue-cells-after-white-spot-syndrome-virus-infection
#9
Meng-Ru Zhao, Chuang Meng, Xiao-Lu Xie, Cheng-Hua Li, Hai-Peng Liu
White spot syndrome virus (WSSV) is one of the most prevalent and widespread viruses in both shrimp and crayfish aquaculture. MicroRNAs (miRNAs) are crucial post-transcriptional regulators and play critical roles in cell differentiation and proliferation, apoptosis, signal transduction and immunity. In this study, miRNA expression profiles were identified via deep sequencing in red claw crayfish Cherax quadricarinatus haematopoietic tissue (Hpt) cell cultures infected with WSSV at both early (i.e., 1 hpi) and late (i...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27663854/characterization-of-a-hemocyte-homeostasis-associated-like-protein-hhap-in-the-freshwater-crayfish-pacifastacus-leniusculus
#10
Kantamas Apitanyasai, Chadanat Noonin, Anchalee Tassanakajon, Irene Söderhäll, Kenneth Söderhäll
Hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus has a distinct role from that of its homolog PmHHAP in the shrimp Penaeus monodon. Knockdown of PlHHAP in vitro using double-stranded RNA (dsRNA) had no effect on the cell morphology of hematopoietic tissue (HPT) cells. The total hemocyte number and caspase activity were unchanged after PlHHAP knockdown in vivo, in contrast to the results found in shrimp. Moreover, suppression of PlHHAP both in vitro and in vivo did not change the mRNA levels of some genes involved in hematopoiesis and hemocyte homeostasis...
November 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27544268/identification-of-an-anti-lipopolysacchride-factor-possessing-both-antiviral-and-antibacterial-activity-from-the-red-claw-crayfish-cherax-quadricarinatus
#11
Feng-Yu Lin, Yan Gao, Hao Wang, Qiu-Xia Zhang, Chang-Lin Zeng, Hai-Peng Liu
It is well-known that anti-lipopolysacchride factors (ALFs) are involved in the recognition and elimination of invading pathogens. In this study, the full-length ALF cDNA sequence of the red claw crayfish Cherax quadricarinatus (termed CqALF) was cloned from a suppression subtractive hybridization library constructed using red claw crayfish hematopoietic tissue cell (Hpt cell) cultures following challenge with white spot syndrome virus (WSSV). The full-length cDNA sequence of CqALF was 863 bp, and the open reading frame encoded 123 amino acids with a signal peptide in the N-terminus and a conserved LPS-binding domain...
October 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27385304/white-spot-syndrome-virus-entry-is-dependent-on-multiple-endocytic-routes-and-strongly-facilitated-by-cq-gabarap-in-a-cme-dependent-manner
#12
Rong-Yuan Chen, Kai-Li Shen, Zhen Chen, Wei-Wei Fan, Xiao-Lu Xie, Chuang Meng, Xue-Jiao Chang, Li-Bing Zheng, Joseph Jeswin, Cheng-Hua Li, Ke-Jian Wang, Hai-Peng Liu
White spot syndrome virus (WSSV) is a lethal pathogen of shrimp and many other crustaceans, including crayfish. However, the molecular mechanism underlying its cellular entry remains elusive due to the lack of shrimp cell lines for viral propagation. Crayfish hematopoietic tissue (Hpt) cell culture was recently established as a good model for WSSV infection study. Here, we showed that multiple endocytic routes, including clathrin-mediated endocytosis (CME), macropinocytosis and caveolae-mediated endocytosis, were indispensably employed for the viral entry into Hpt cell of the crayfish Cherax quadricarinatus...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27339892/reactive-oxygen-species-affect-transglutaminase-activity-and-regulate-hematopoiesis-in-a-crustacean
#13
Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll, Chadanat Noonin
Reactive oxygen species (ROS) serve as a prime signal in the commitment to hematopoiesis in both mammals and Drosophila In this study, the potential function of ROS during hematopoiesis in the crayfish Pacifastacus leniusculus was examined. The antioxidant N-acetylcysteine (NAC) was used to decrease ROS in both in vivo and in vitro experiments. An increase in ROS was observed in the anterior proliferation center (APC) after LPS injection. In the absence of NAC, the LPS-induced increase in ROS levels resulted in the rapid restoration of the circulating hemocyte number...
August 19, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26845698/proteomic-analysis-by-itraq-in-red-claw-crayfish-cherax-quadricarinatus-hematopoietic-tissue-cells-post-white-spot-syndrome-virus-infection
#14
Joseph Jeswin, Xiao-lu Xie, Qiao-lin Ji, Ke-jian Wang, Hai-peng Liu
To elucidate proteomic changes of Hpt cells from red claw crayfish, Cherax quadricarinatus, we have carried out isobaric tags for relative and absolute quantitation (iTRAQ) of cellular proteins at both early (1 hpi) and late stage (12 hpi) post white spot syndrome virus (WSSV) infection. Protein database search revealed 594 protein hits by Mascot, in which 17 and 30 proteins were present as differentially expressed proteins at early and late viral infection, respectively. Generally, these differentially expressed proteins include: 1) the metabolic process related proteins in glycolysis and glucogenesis, DNA replication, nucleotide/amino acid/fatty acid metabolism and protein biosynthesis; 2) the signal transduction related proteins like small GTPases, G-protein-alpha stimulatory subunit, proteins bearing PDZ- or 14-3-3-domains that help holding together and organize signaling complexes, casein kinase I and proteins of the MAP-kinase signal transduction pathway; 3) the immune defense related proteins such as α-2 macroglobulin, transglutaminase and trans-activation response RNA-binding protein 1...
March 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/25541079/crayfish-hematopoietic-tissue-cells-but-not-hemocytes-are-permissive-for-white-spot-syndrome-virus-replication
#15
Junjun Wu, Fang Li, Jiajun Huang, Limei Xu, Feng Yang
Hemocytes are the major immune cells of crustaceans which are believed to be essential for the pathogenesis of white spot syndrome virus (WSSV) infection. Crayfish hemocytes and hematopoietic tissue (HPT) cells have been found to be susceptible to WSSV infection, but the procedure of WSSV infection to both cell types has not yet been carefully investigated. In this study, we analyzed the infection and proliferation of WSSV in crayfish hemocytes as well as HPT cells in detail through transmission electronic microscopy (TEM)...
March 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/23565293/%C3%AE-thymosins-and-hemocyte-homeostasis-in-a-crustacean
#16
Netnapa Saelee, Chadanat Noonin, Benjamas Nupan, Kingkamon Junkunlo, Amornrat Phongdara, Xionghui Lin, Kenneth Söderhäll, Irene Söderhäll
Thymosin proteins are well known for their actin-binding activity. Thymosin beta 4 (Tβ4) has been associated with biological activities in tissue repair and cell migration via interaction with ATP-synthase in vertebrates, while the information of similar thymosin functions in invertebrates is limited. We have shown previously that ATP-synthase is present on the surface of crayfish hematopoietic tissue (HPT) cells, and that astakine 1 (Ast1, an invertebrate cytokine) was found to interact with this β-subunit of ATP synthase...
2013: PloS One
https://www.readbyqxmd.com/read/22108619/optimized-production-of-scygonadin-in-pichia-pastoris-and-analysis-of-its-antimicrobial-and-antiviral-activities
#17
Hui Peng, Hai-Peng Liu, Bei Chen, Hua Hao, Ke-Jian Wang
The crab antimicrobial peptide scygonadin is confirmed to have antimicrobial activity against bacteria and it is probably associated with the reproductive immunity in Scylla paramamosain. To obtain large quantity of scygonadin for further biological assays, a 306 bp cDNA sequence encoding the mature peptide of scygonadin was cloned into a secretion vector of pPIC9K, and a high-level of the recombinant scygonadin was achieved in Pichia pastoris. The optimal expression condition was determined as incubation with 0...
March 2012: Protein Expression and Purification
https://www.readbyqxmd.com/read/21598394/two-novel-ficolin-like-proteins-act-as-pattern-recognition-receptors-for-invading-pathogens-in-the-freshwater-crayfish-pacifastacus-leniusculus
#18
Chenglin Wu, Kenneth Söderhäll, Irene Söderhäll
To isolate pathogen-associated molecular patterns (PAMPs)-binding molecules, the bacterium, Staphylococcus aureus was used as an affinity matrix to find bacteria-binding proteins in the plasma of the freshwater crayfish, Pacifastacus leniusculus. Two new bacteria-binding ficolin-like proteins (FLPs) were identified by 2-DE and MS analysis. The FLPs have a fibrinogen-related domain (FReD) in their C-terminal and a repeat region in their N-terminal regions with putative structural similarities to the collagen-like domain of vertebrate ficolins and mannose binding lectins (MBLs)...
June 2011: Proteomics
https://www.readbyqxmd.com/read/21396955/differential-gene-expression-profile-from-haematopoietic-tissue-stem-cells-of-red-claw-crayfish-cherax-quadricarinatus-in-response-to-wssv-infection
#19
Hai-peng Liu, Rong-yuan Chen, Qiu-xia Zhang, Hui Peng, Ke-jian Wang
White spot syndrome virus (WSSV) is one of the most important viral pathogens in crustaceans. During WSSV infection, multiple cell signaling cascades are activated, leading to the generation of antiviral molecules and initiation of programmed cell death of the virus infected cells. To gain novel insight into cell signaling mechanisms employed in WSSV infection, we have used suppression subtractive hybridization (SSH) to elucidate the cellular response to WSSV challenge at the gene level in red claw crayfish haematopoietic tissue (Hpt) stem cell cultures...
July 2011: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/21220699/invertebrate-hematopoiesis-an-astakine-dependent-novel-hematopoietic-factor
#20
COMPARATIVE STUDY
Xionghui Lin, Kenneth Söderhäll, Irene Söderhäll
A novel factor, named crustacean hematopoietic factor (CHF), was identified from a library of suppression subtractive hybridization with the aim to find downstream genes of an invertebrate cytokine, astakine 1, in the freshwater crayfish Pacifastacus leniusculus. CHF is a small cysteine-rich protein (∼9 kDa) with high similarity to the N-terminal region of vertebrate CRIM1 in containing an insulin growth factor binding protein variant motif with unknown function. CHF was found to be induced in primary cell cultures of crayfish hematopoietic tissue (Hpt) cells (precursors of crayfish blood cells) after treatment with astakine 1...
February 15, 2011: Journal of Immunology: Official Journal of the American Association of Immunologists
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