Yueli Wang, Aodi Wu, Zhenyu Xu, Huan Zhang, Honghuan Li, Shuangshuang Fu, Yajing Liu, Lijin Cui, Yuhe Miao, Yong Wang, Kaiat Zhumanov, Yimei Xu, Jinliang Sheng, Jihai Yi, Chuangfu Chen
Brucellosis, a zoonosis caused by Brucella, is highly detrimental to both humans and animals. Most existing vaccines are live attenuated vaccines with safety flaws for people and animals. Therefore, it is advantageous to design a multi-epitope subunit vaccine (MEV) to prevent Brucella infection. To this end, we applied a reverse vaccinology approach. Six cytotoxic T cell (CTL) epitopes, seven T helper cell (HTL) epitopes, and four linear B cell epitopes from CU/ZN-SOD, Omp31, and BP26 were obtained. We linked the CTL, HTL, B-cell epitopes, the appropriate CTB molecular adjuvant, and the universal T helper lymphocyte epitope, PADRE, with linkers AAY, GPPGG, and KK, respectively...
December 18, 2023: International Immunopharmacology