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https://www.readbyqxmd.com/read/29128919/genome-wide-prediction-of-potential-vaccine-candidates-for-campylobacter-jejuni-using-reverse-vaccinology
#1
Richa Jain, Sarita Singh, Santosh Kumar Verma, Ankit Jain
Campylobacteriosis is a deadly disease which has developed resistance to most of the available chemotherapeutic agents. Although various studies provide evidence of acquired immunity following exposure to Campylobacter jejuni, no effective vaccine has been developed, still. Hence, there is an urgent need to identify potential vaccine candidates for Campylobacter species. In the proposed study, Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) was taken and computational approach was employed to screen C...
November 11, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/29122004/identification-and-characterization-of-serovar-independent-immunogens-in-actinobacillus-pleuropneumoniae
#2
Fabio Antenucci, Cyrielle Fougeroux, Janine T Bossé, Zofia Magnowska, Camille Roesch, Paul Langford, Peter Johannes Holst, Anders Miki Bojesen
Despite numerous actions to prevent disease, Actinobacillus pleuropneumoniae (A. pleuropneumoniae) remains a major cause of porcine pleuropneumonia, resulting in economic losses to the swine industry worldwide. In this paper, we describe the utilization of a reverse vaccinology approach for the selection and in vitro testing of serovar-independent A. pleuropneumoniae immunogens. Potential immunogens were identified in the complete genomes of three A. pleuropneumoniae strains belonging to different serovars using the following parameters: predicted outer-membrane subcellular localization; ≤ 1 trans-membrane helices; presence of a signal peptide in the protein sequence; presence in all known A...
November 9, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29119233/potential-outer-membrane-protein-candidates-for-vaccine-development-against-the-pathogen-vibrio-anguillarum-a-reverse-vaccinology-based-identification
#3
Pallavi Baliga, Malathi Shekar, Moleyur Nagarajappa Venugopal
Reverse vaccinology is a widely used approach that has facilitated the rapid identification of vaccine candidates suitable in vaccine development for pathogens. Vibrio anguillarum is a major pathogen responsible for vibriosis in fish and shellfish leading to huge economic losses to the aquaculture industry. Although commercial vaccines are available for fish against this bacterium they have their own limitations. In this study, we used the reverse vaccinology strategy to screen and identify V. anguillarum outer membrane proteins (OMPs) that could serve as vaccine candidates...
November 8, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28930522/advances-in-tick-vaccinology-in-brazil-from-gene-expression-to-immunoprotection
#4
Renato Andreotti, Poliana Fernanda Giachetto, Rodrigo Casquero Cunha
Rhipicephalus (Boophilus) microplus has substantial economic impact on the cattle breeding industry and, chemical control and tick resistance development are the major concern. There is a worldwide search for new options, and control using vaccines has been the main focus nowadays. Studies performed in Brazil found that Bm86-based immunization of bovines reduced the infestation of R. (B.) microplus of vaccinated bovines by 45% to 60%. Native Boophilusmicroplus tripsin inhibitors (BmTIs) with trypsin-, kallikrein-, and elastase-inhibiting activities have been used as immunogens in bovines reaching 72...
January 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28919551/identification-of-potential-antigens-from-non-classically-secreted-proteins-and-designing-novel-multitope-peptide-vaccine-candidate-against-brucella-melitensis-through-reverse-vaccinology-and-immunoinformatics-approach
#5
Udayakumar S Vishnu, Jagadesan Sankarasubramanian, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Brucella melitensis is an intracellular pathogen resides in the professional and non-professional phagocytes of the host, causing zoonotic disease brucellosis. The stealthy nature of the Brucella makes it's highly pathogenic, and it is hard to eliminate the bacteria completely from the infected host. Hitherto, no licensed vaccines are available for human brucellosis. In this study, we identified potential antigens for vaccine development from non-classically secreted proteins through reverse vaccinology approach...
September 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28903988/the-use-of-reverse-vaccinology-in-the-design-and-construction-of-nano-glycoconjugate-vaccines-against-burkholderia-pseudomallei
#6
Laura A Muruato, Daniel Tapia, Christopher L Hatcher, Mridul Kalita, Paul J Brett, Anthony E Gregory, James E Samuel, Richard W Titball, Alfredo G Torres
Burkholderia pseudomallei (Bpm) is a Gram negative, facultative intracellular pathogen that causes the disease melioidosis in humans and other mammals. Respiratory infection with B. pseudomallei leads to a fulminant and often fatal disease. It has previously been shown that glycoconjugate vaccines can provide significant protection against lethal challenge; however, the limited number of known Burkholderia antigens has slowed progress towards vaccine development. The objective of this study was to identify novel antigens and evaluate their protective capacity when incorporated into a nano-glycoconjugate vaccine platform...
September 13, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28893639/on-the-application-of-reverse-vaccinology-to-parasitic-diseases-a-perspective-on-feature-selection-and-ranking-of-vaccine-candidates
#7
Stephen J Goodswen, Paul J Kennedy, John T Ellis
Reverse vaccinology has the potential to rapidly advance vaccine development against parasites, but it is unclear which features studied in silico will advance vaccine development. Here we consider Neospora caninum which is a globally distributed protozoan parasite causing significant economic and reproductive loss to cattle industries worldwide. The aim of this study was to use a reverse vaccinology approach to compile a worthy vaccine candidate list for N. caninum, including proteins containing pathogen-associated molecular patterns to act as vaccine carriers...
October 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28867962/vaccine-research-and-development-tuberculosis-as-a-global-health-threat
#8
REVIEW
Mohammed Maikudi Usman, Salmah Ismail, Teow Chong Teoh
One of the aims of the World Health Organisation (WHO) Millennium Development Goals (MDG) is to reduce the number of cases of tuberculosis (TB) infection by the year 2015. However, 9 million new cases were reported in 2013, with an estimated 480,000 new cases of multi-drug resistant tuberculosis (MDR-TB) globally. Bacille Calmette-Guérin (BCG) is the most available and currently used candidate vaccine against tuberculosis; it prevents childhood TB, but its effectiveness against pulmonary TB in adults and adolescents is disputed...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/28826780/towards-a-peptide-based-vaccine-against-shigella-sonnei-a-subtractive-reverse-vaccinology-based-approach
#9
Shehneela Baseer, Sajjad Ahmad, Kara E Ranaghan, Syed Sikander Azam
Shigella sonnei is one of the major causes of shigellosis in technically advanced countries and reports of its unprecedented increase are published from the Middle East, Latin America, and Asia. The pathogen exhibits resistance against first and second line antibiotics which highlights the need for the development of an effective broad-spectrum vaccine. A computational based approach comprising subtractive reverse vaccinology was used for the identification of potential peptide-based vaccine candidates in the proteome of S...
August 18, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28739221/structure-and-computation-in-immunoreagent-design-from-diagnostics-to-vaccines
#10
REVIEW
Louise Gourlay, Claudio Peri, Martino Bolognesi, Giorgio Colombo
Novel immunological tools for efficient diagnosis and treatment of emerging infections are urgently required. Advances in the diagnostic and vaccine development fields are continuously progressing, with reverse vaccinology and structural vaccinology (SV) methods for antigen identification and structure-based antigen (re)design playing increasingly relevant roles. SV, in particular, is predicted to be the front-runner in the future development of diagnostics and vaccines targeting challenging diseases such as AIDS and cancer...
December 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28646586/vaccine-candidate-discovery-for-the-next-generation-of-malaria-vaccines
#11
REVIEW
James Tuju, Gathoni Kamuyu, Linda M Murungi, Faith H A Osier
Although epidemiological observations, IgG passive transfer studies and experimental infections in humans all support the feasibility of developing highly effective malaria vaccines, the precise antigens that induce protective immunity remain uncertain. Here, we review the methodologies applied to vaccine candidate discovery for Plasmodium falciparum malaria from the pre- to post-genomic era. Probing of genomic and cDNA libraries with antibodies of defined specificities or functional activity predominated the former, whereas reverse vaccinology encompassing high throughput in silico analyses of genomic, transcriptomic or proteomic parasite data sets is the mainstay of the latter...
October 2017: Immunology
https://www.readbyqxmd.com/read/28630127/deciphering-function-of-new-gonococcal-vaccine-antigens-using-phenotypic-microarrays
#12
Benjamin I Baarda, Sarah Emerson, Philip J Proteau, Aleksandra E Sikora
The function and extracellular location of cell envelope proteins make them attractive candidates for developing vaccines against bacterial diseases, including challenging, drug-resistant pathogens such as Neisseria gonorrhoeae Proteomics-driven reverse vaccinology delivered multiple gonorrhea vaccine candidates; however, the biological functions of many of them remain to be elucidated. Herein, the functions of six gonorrhea vaccine candidates - NGO2121, NGO1985, NGO2054, NGO2111, NGO1205, and NGO1344 - in cell envelope homeostasis were probed using phenotype microarrays with 1,056 conditions and a ΔbamE mutant (Δngo1780) as a reference of perturbed outer membrane integrity...
June 19, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28587729/glycolysis-related-proteins-are-broad-spectrum-vaccine-candidates-against-aquacultural-pathogens
#13
Xiaohong Liu, Jiamin Sun, Haizhen Wu
Reverse vaccinology (RV) has become a popular method for developing vaccines. Although Edwardsiella tarda is deemed to be an important fish pathogen, so far, no reports have used a genome-based approach to screen vaccine candidates against E. tarda. In the current study, protective antigens of E. tarda were screened using RV. Large-scale cloning, expression and purification of potential candidates were carried out, and their immunoprotective potential was evaluated. A candidate fructose-bisphosphate aldolase (FBA) exhibited broad spectrum protection, as did another glycolysis-related protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which we reported previously, indicating the potential of other glycolysis-related proteins of E...
June 3, 2017: Vaccine
https://www.readbyqxmd.com/read/28584449/epitope-design-of-l1-protein-for-vaccine-production-against-human-papilloma-virus-types-16-and-18
#14
Sunanda Baidya, Rasel Das, Md Golam Kabir, Md Arifuzzaman
Cervical cancer accounts for about two-thirds of all cancer cases linked etiologically to Human Papilloma Virus (HPV). 15 oncogenic HPV types can cause cervical cancer, of which HPV16 and HPV18 combinedly account for about 70% of it. So, effective epitope design for the clinically relevant HPV types 16 and 18 would be of major medical benefit. Here, a comprehensive analysis is carried out to predict the epitopes against HPV types 16 and 18 through "reverse vaccinology" approach. We attempted to identify the evolutionarily conserved regions of major capsid protein (L1) as well as minor capsid protein (L2) of HPV and designed epitopes within these regions...
2017: Bioinformation
https://www.readbyqxmd.com/read/28555545/identification-of-cross-reacting-t-cell-epitopes-in-structural-and-non-structural-proteins-of-swine-and-pandemic-h1n1-influenza-a-virus-strains-in-pigs
#15
Massimiliano Baratelli, Lasse Eggers Pedersen, Ramona Trebbien, Lars Erik Larsen, Gregers Jungersen, Esther Blanco, Jens Nielsen, Maria Montoya
Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between 'avian-like' H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs...
May 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28496441/discovery-of-novel-leptospirosis-vaccine-candidates-using-reverse-and-structural-vaccinology
#16
André Alex Grassmann, Frederico Schmitt Kremer, Júlia Cougo Dos Santos, Jéssica Dias Souza, Luciano da Silva Pinto, Alan John Alexander McBride
Leptospira spp. are diderm (two membranes) bacteria that infect mammals causing leptospirosis, a public health problem with global implications. Thousands of people die every year due to leptospirosis, especially in developing countries with tropical climates. Prophylaxis is difficult due to multiple factors, including the large number of asymptomatic hosts that transmit the bacteria, poor sanitation, increasing numbers of slum dwellers, and the lack of an effective vaccine. Several leptospiral recombinant antigens were evaluated as a replacement for the inactivated (bacterin) vaccine; however, success has been limited...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28487172/prediction-of-vaccine-candidates-against-pseudomonas-aeruginosa-an-integrated-genomics-and-proteomics-approach
#17
Muhammad Ibrahim Rashid, Anam Naz, Amjad Ali, Saadia Andleeb
Pseudomonas aeruginosa is among top critical nosocomial infectious agents due to its persistent infections and tendency for acquiring drug resistance mechanisms. To date, there is no vaccine available for this pathogen. We attempted to exploit the genomic and proteomic information of P. aeruginosa though reverse-vaccinology approaches to unveil the prospective vaccine candidates. P. aeruginosa strain PAO1 genome was subjected to sequential prioritization approach following genomic, proteomics and structural analyses...
July 2017: Genomics
https://www.readbyqxmd.com/read/28369428/novel-approaches-to-neisseria-meningitidis-vaccine-design
#18
Myron Christodoulides, John Heckels
A range of vaccines is available for preventing life-threatening diseases caused by infection with Neisseria meningitidis (meningococcus, Men). Capsule polysaccharide (CPS)-conjugate vaccines are successful prophylactics for serogroup MenA, MenC, MenW and MenY infections, and outer membrane vesicle (OMV) vaccines have been used successfully for controlling clonal serogroup MenB infections. MenB vaccines based on recombinant proteins identified by reverse vaccinology (Bexsero™) and proteomics (Trumenba™) approaches have recently been licensed and Bexsero™ has been introduced into the UK infant immunisation programme...
April 1, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28352257/a-novel-pan-genome-reverse-vaccinology-approach-employing-a-negative-selection-strategy-for-screening-surface-exposed-antigens-against-leptospirosis
#19
LingBing Zeng, Dongliang Wang, NiYa Hu, Qing Zhu, Kaishen Chen, Ke Dong, Yan Zhang, YuFeng Yao, XiaoKui Guo, Yung-Fu Chang, YongZhang Zhu
Reverse vaccinology (RV) has been widely used for screening of surface-exposed proteins (PSEs) of important pathogens, including outer membrane proteins (OMPs), and extracellular proteins (ECPs) as potential vaccine candidates. In this study, we applied a novel RV negative strategy and a pan-genome analysis for screening of PSEs from 17 L. interrogans strains covering 11 predominately epidemic serovars and 17 multilocus typing (MLST) sequence types (STs) worldwide. Our results showed, for instance, out of a total of 633 predicted PSEs in strain 56601, 92...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28337203/a-universal-vaccine-against-leptospirosis-are-we-going-in-the-right-direction
#20
André Alex Grassmann, Jéssica Dias Souza, Alan John Alexander McBride
Leptospirosis is the most widespread zoonosis in the world and a neglected tropical disease estimated to cause severe infection in more than one million people worldwide every year that can be combated by effective immunization. However, no significant progress has been made on the leptospirosis vaccine since the advent of bacterins over 100 years. Although protective against lethal infection, particularly in animals, bacterin-induced immunity is considered short term, serovar restricted, and the vaccine can cause serious side effects...
2017: Frontiers in Immunology
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