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https://www.readbyqxmd.com/read/28226015/-knowledge-attitude-and-practice-on-childhood-immunization-personnel-in-teresina-pi-brazil-2015
#1
Clara Ananda Pimentel de Sousa Santos, Rosana Dos Santos Costa, Jéssica Loureiro Mendes Silva, Maria Dos Remédios Farias Dos Santos, Bruna Lays Ferreira Gomes
OBJECTIVE: to describe the knowledge, attitude and practice of vaccinators toward childhood vaccination. METHODS: this is a descriptive census study, conducted in 2015 with vaccinators from vaccination rooms of the urban area of Teresina, Piauí, Brazil; data were collected through a questionnaire. RESULTS: 70 vaccinators were interviewed; most answers related to knowledge and attitude of the vaccinators toward childhood vaccination were considered inadequate (n=58 and n=39, respectively); with regard to practice, there were 43 adequate answers; there was no statistically significant association between the variable knowledge and the variables attitude and practice...
January 2017: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/28226004/guillain-barr%C3%A3-syndrome-and-other-neurological-manifestations-possibly-related-to-zika-virus-infection-in-municipalities-from-bahia-brazil-2015
#2
Juliane Maria Alves Siqueira Malta, Alexander Vargas, Priscila Leal E Leite, Jadher Percio, Giovanini Evelim Coelho, Andréa Helena Argolo Ferraro, Tânia Maria de Oliveira Cordeiro, Jesângeli de Sousa Dias, Eduardo Saad
Objective: to describe the reported cases of Guillain-Barré Syndrome (GBS) and other neurological manifestations with a history of dengue, chikungunya or Zika virus infections, in the Metropolitan Region of Salvador and in the municipality of Feira de Santana, Brazil. Methods: this is a descriptive study with data of an investigation conducted by the epidemiological surveillance from March to August 2015; to confirm the neurological manifestations, medical diagnosis records were considered, and to prior infection, clinical and laboratory criteria were used...
January 2017: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/28222355/neuroimaging-of-major-depression-in-parkinson-s-disease-cortical-thickness-cortical-and-subcortical-volume-and-spectroscopy-findings
#3
Marcos Hortes N Chagas, Vitor Tumas, Márcio A Pena-Pereira, João Paulo Machado-de-Sousa, Antonio Carlos Dos Santos, Rafael Faria Sanches, Jaime E C Hallak, José Alexandre S Crippa
Depression is the most common psychiatric disorder in Parkinson's disease (PD). The aim of this study was to compare PD patients with current Major Depressive Disorder (MDD), lifetime MDD, and no MDD using three neuroimaging techniques. A total of 43 PD patients were selected and divided into three groups: (i) current MDD (n = 15), (ii) previous MDD without current MDD (n = 10); and (iii) control group (no current or lifetime MDD; n = 18). All participants underwent magnetic resonance imaging to evaluate cortical thickness, cortical and subcortical volume, and spectroscopy in the bilateral putamen and cingulate cortex...
February 13, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28219105/effects-of-resistance-training-volume-on-mmps-in-circulation-muscle-and-adipose-tissue
#4
Ivo Vieira de Sousa Neto, Ramires Alsamir Tibana, Dahan da Cunha Nascimento, Denis César Leite Vieira, Joao Luiz Quagliotti Durigan, Guilherme Borges Pereira, James Wilfred Navalta, Rita de Cássia Marqueti, Jonato Prestes
The aim of this study was to investigate the effects of different resistance training (RT) volumes on MMP activity in skeletal muscle, visceral adipose tissue and circulation. 21 Wistar rats were randomly divided into 3 groups (n=7 per group): sedentary control (SC); RT with 4 ladder climbs (RT-4; 50, 75, 90 and 100% of their maximal carrying capacity) and RT with 8 ladder climbs (RT-8 with 2 sets for each load). The 8-week RT consisted of climbing a 1.1-m vertical ladder with weights secured to the animals' tails...
February 20, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28215506/radial-scar-of-the-breast-is-it-possible-to-avoid-surgery
#5
A I Ferreira, S Borges, A Sousa, C Ribeiro, A Mesquita, P C Martins, M Peyroteo, N Coimbra, C Leal, P Reis, J A Sousa
INTRODUCTION: Breast radial scar (RS) management remains controversial. The need for surgical excision is supported by the concern of an associated high-grade lesion missed in the biopsy. The aim of this study was to assess histologic upgrade rate after a percutaneous biopsy, to determine if vacuum assisted biopsy prevents the need for subsequent RS surgical resection and to evaluate the upgrade risk factors. PATIENTS AND METHODS: This was a uni-institutional retrospective study of consecutive patients with RS histologically diagnosed from January 2010 to December 2015...
February 3, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28214224/escherichia-coli-sequence-type-73-as-a-cause-of-community-acquired-urinary-tract-infection-in-men-and-women-in-rio-de-janeiro-brazil
#6
Ana Paula de Souza da-Silva, Viviane Santos de Sousa, Natacha Martins, Rubens Clayton da Silva Dias, Raquel Regina Bonelli, Lee W Riley, Beatriz Meurer Moreira
Escherichia coli clones ST131, ST69, ST95, and ST73 are frequent causes of urinary tract infections (UTI) and bloodstream infections. Specific clones and virulence profiles of E. coli causing UTI in men has been rarely described. The aim of this study was to characterize patient and clonal characteristics of community-acquired UTI caused by E. coli in men (n=12) and women (n=127) in Rio de Janeiro, Brazil, complementing a previous work. We characterized isolates in phylogenetic groups, ERIC2-PCR and PFGE types, MLST, genome similarity and virulence gene-profiles...
February 7, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28210027/klapp-method-effect-on-idiopathic-scoliosis-in-adolescents-blind-randomized-controlled-clinical-trial
#7
Diego De Sousa Dantas, Sanderson José Costa De Assis, Marina Pegoraro Baroni, Johnnatas Mikael Lopes, Enio Walker Azevedo Cacho, Roberta De Oliveira Cacho, Silvana Alves Pereira
[Purpose] To estimate the effect of Klapp method on idiopathic scoliosis in school students. [Subjects and Methods] A single-blind randomized clinical trial with 22 students randomly divided into intervention group (n=12) and inactive control group (n=10). Exercise protocol consisted of Klapp method, 20 sessions, three times a week for intervention group, and inactivity for control group. Dorsal muscle strength was measured by dynamometer; body asymmetries and gibbosity angles were measured by biophotogrammetry...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28197003/celebrity-suicide-and-its-effect-on-further-media-reporting-and-portrayal-of-suicide-an-exploratory-study
#8
Devavrat Harshe, Sagar Karia, Sneha Harshe, Nilesh Shah, Gurudas Harshe, Avinash De Sousa
BACKGROUND: Suicide is a grave mental health problem in India, and suicide rates in India have risen over the past decades. Suicide reporting by the media is a common cause for spurts of suicides that may occur from time to time. The aim of the present study was to assess the change in trends in media reporting of suicide after a celebrity suicide. METHODOLOGY: Suicide by the renowned actor Robin Williams was selected as the reference case. The top three Indian daily newspapers published in English having the highest circulation as per the Registrar of Newspapers, Government of India report were selected to be scanned in the study...
October 2016: Indian Journal of Psychiatry
https://www.readbyqxmd.com/read/28196677/molecular-modeling-docking-and-dynamics-simulations-of-the-dioclea-lasiophylla-mart-ex-benth-seed-lectin-an-edematogenic-and-hypernociceptive-protein
#9
Vanir Reis Pinto-Junior, Vinicius Jose Silva Osterne, Mayara Queiroz Santiago, Claudia Figueiredo Lossio, Celso Shiniti Nagano, Cintia Renata Costa Rocha, Jessica Catarine Frutuoso Nascimento, Francisco Lucas Faustino Nascimento, Ivanice Bezerra Silva, Antonia Simoni Oliveira, Jorge Luis Almeida Correia, Rodrigo Bainy Leal, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada, Kyria Santiago Nascimento
Lectins are proteins, or glycoproteins, capable of reversibly binding to specific mono- or oligosaccharides via a noncatalytic domain. The Diocleinae subtribe presents lectins with high structural similarity, but different effects based on biological activity assays. This variability results from small structural differences. Therefore, in this context, the present study aimed to perform a structural analysis of the lectin from Dioclea lasiophylla Mart. ex Benth seeds (DlyL) and evaluate its inflammatory effect...
February 11, 2017: Biochimie
https://www.readbyqxmd.com/read/28191743/manual-thrombectomy-efficiency-in-relationship-to-the-area-at-risk-in-patients-with-myocardial-infarction-with-timi-0-1-coronary-flow-insights-from-an-all-comers-registry
#10
André Luz, Inês Silveira, Bruno Brochado, Patrícia Rodrigues, Maria João Sousa, Raquel Santos, Maria Trêpa, Mário Santos, João Silveira, Severo Torres, Adelino F Leite-Moreira, Henrique Carvalho
OBJECTIVES: To review the effectiveness of manual thrombectomy (MT) in a series of patients with ST-elevation myocardial infarction (STEMI) exclusively presenting with TIMI 0-1 flow undergoing percutaneous coronary intervention (PCI), in accordance to the angiographically estimated area at risk (AAR). Second, to assess major in-hospital clinical events, emphasizing neurological outcomes. BACKGROUND: The routine utilization of MT in STEMI is not recommended. However, in recent trials, a significant proportion of patients had neither large thrombus burden nor a totally occluded coronary segment, neutralizing the expected benefits of MT...
February 13, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28185763/bariatric-surgery-attenuates-colitis-in-an-obese-murine-model
#11
Shiri Li, Alessio Vinci, Judith Behnsen, Chunmei Cheng, Stefan Jellbauer, Manuela Raffatellu, Kyle M Sousa, Robert Edwards, Ninh T Nguyen, Michael J Stamos, Alessio Pigazzi
BACKGROUND: Obesity and inflammatory bowel disease (IBD) represent chronic inflammatory conditions. Bariatric surgery improves some obesity-related co-morbidities, but the effects of bariatric surgery on IBD have not been well studied. OBJECTIVES: To examine if bariatric surgery may attenuate colitis in an obese murine model of IBD and study the mechanisms underlying the postsurgical amelioration of intestinal inflammation. SETTING: University of California Irvine, Department of Surgery and Microbiology laboratories...
December 20, 2016: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28185415/relationship-between-clinical-evaluation-and-ultrasound-assessment-of-rheumatoid-arthritis-patients-using-a-12-joint-score
#12
Marcos Cerqueira, Filipa Teixeira, Joana Sousa Neves, Daniela Peixoto, Maria Carmo Afonso, José A Costa
AIM: To identify if the use of a systematic ultrasound (US) evaluation has relevance in the determination of disease activity in rheumatoid arthritis patients on biological disease-modifying anti-rheumatic drug treatment. METHODS: A 12 joint US assessment was performed on the same day of the routine clinical examination. Both Grey-scale (GS) and Power Doppler (PD) were graded semi-quantitatively (0-3 scale). RESULTS: Forty-one patients were included...
February 9, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28177723/fish-oil-and-treadmill-exercise-have-age-dependent-effects-on-episodic-memory-and-oxidative-state-of-the-hippocampus
#13
Patrícia Fortes Cavalcanti de Macêdo, Janatar Stella Vasconcelos de Melo, Laís Alves Ribeiro Costa, Glauber Rudá Feitoza Braz, Shirley M de Sousa, Cláudia J Lagranha, Manuella Batista-de-Oliveira Hornsby
There is a growing interest to better understand how lifestyle choices can improve memory functions. Treadmill exercise (Ex) and long-chain n-3 polyunsaturated fatty acids (PUFAs) found in fish oil (Fo) are able to stimulate hippocampal antioxidant defenses and improve memory. The aim was to test whether (Fo) and (Ex) can improve rat's performance on memory tasks and optimize hippocampal antioxidant state in an age-dependent manner. Therefore, young and adult rats were exercised and received (Fo) during four weeks...
January 9, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28166976/biopsy-of-bovine-embryos-produced-in%C3%A2-vivo-and-in%C3%A2-vitro-does-not-affect-pregnancy-rates
#14
Regivaldo Vieira de Sousa, Célia Regina da Silva Cardoso, Guilberth Butzke, Margot Alves Nunes Dode, Rodolfo Rumpf, Maurício Machaim Franco
Assisted reproductive techniques have significantly contributed to animal breeding programs. Similarly, genomics has provided important information and tools to improve the accuracy of selection. However, the greatest benefits of those tools can only be expected when they are combined, allowing animals to be selected accurately early in life. Therefore, obtaining DNA samples from embryos without compromising their viability is essential for the consolidation of preimplantation genomic selection. We aimed to evaluate the effect on the gestation rate of conducting a biopsy of in vivo (VV) and in vitro-produced (IVP) bovine embryos...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28157423/effect-of-nd-yag-and-co2-laser-irradiation-on-prevention-of-enamel-demineralization-in-orthodontics-in-vitro-study
#15
Renato Siva Paulos, Priscila Yumi Seino, Karen Akemi Fukushima, Marcia Martins Marques, Fernanda Campos Sousa de Almeida, Karen Muller Ramalho, Patricia Moreira de Freitas, Aldo Brugnera, Maria Stella Moreira
OBJECTIVE: The aim of this study was to investigate Nd:YAG and CO2 laser effects in the prevention of demineralization in deeper layers of enamel via successive acid challenge cycles. BACKGROUND DATA: Lasers are promising in the prevention of enamel demineralization around the orthodontic brackets; however, there are very few studies that evaluate if the effects of treatment could be extended after successive acid challenge cycles due to permanent enamel structural alterations...
January 18, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28155169/nanosilicalites-as-support-for-%C3%AE-glucosidases-covalent-immobilization
#16
Y Carvalho, J M A R Almeida, P N Romano, K Farrance, P Demma Carà, N Pereira, J A Lopez-Sanchez, E F Sousa-Aguiar
Many different materials have been tested for β-glucosidases immobilization. Such materials, however, often show a poor activity related to a low surface area of the support or even enzyme hindrance caused by entrapment inside porous matrix. In this context, the use of nanosized zeolites as enzymes support is quite new and may be an interesting alternative. The present work evaluates the immobilization of β-glucosidases in nanosized silicalites by covalent coupling. The new biocatalyst was able to convert 100% of cellobiose into glucose in 18 h at 50 °C and pH 5, retaining 85% of its activity after five cycles of reuse...
February 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28153694/production-in-pichia-pastoris-antifungal-activity-and-crystal-structure-of-a-class-i-chitinase-from-cowpea-vigna-unguiculata-insights-into-sugar-binding-mode-and-hydrolytic-action
#17
Patrícia G Castro Landim, Tuana O Correia, Fredy D A Silva, Denise R Nepomuceno, Helen P S Costa, Humberto M Pereira, Marina D P Lobo, Frederico B M B Moreno, José Brandão-Neto, Suelen C Medeiros, Ilka M Vasconcelos, José T A Oliveira, Bruno L Sousa, Ito L Barroso-Neto, Valder N Freire, Cristina P S Carvalho, Ana C O Monteiro-Moreira, Thalles B Grangeiro
A cowpea class I chitinase (VuChiI) was expressed in the methylotrophic yeast P. pastoris. The recombinant protein was secreted into the culture medium and purified by affinity chromatography on a chitin matrix. The purified chitinase migrated on SDS-polyacrylamide gel electrophoresis as two closely-related bands with apparent molecular masses of 34 and 37 kDa. The identity of these bands as VuChiI was demonstrated by mass spectrometry analysis of tryptic peptides and N-terminal amino acid sequencing. The recombinant chitinase was able to hydrolyze colloidal chitin but did not exhibit enzymatic activity toward synthetic substrates...
January 30, 2017: Biochimie
https://www.readbyqxmd.com/read/28150103/purification-biochemical-characterization-and-amino-acid-sequence-of-a-novel-type-of-lectin-from-aplysia-dactylomela-eggs-with-antibacterial-antibiofilm-potential
#18
Rômulo Farias Carneiro, Renato Cézar Farias Torres, Renata Pinheiro Chaves, Mayron Alves de Vasconcelos, Bruno Lopes de Sousa, André Castelo Rodrigues Goveia, Francisco Vassiliepe Arruda, Maria Nágila Carneiro Matos, Helena Matthews-Cascon, Valder Nogueira Freire, Edson Holanda Teixeira, Celso Shiniti Nagano, Alexandre Holanda Sampaio
A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HCl-activated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 10(6) M(-1)). The primary structure of ADEL consists of 217 residues, including 11 half-cystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular mass of 57,228 ± 2 Da, as determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry...
February 1, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28146218/influence-of-solvents-on-the-bond-strength-of-resin-sealer-to-intraradicular-dentin-after-retreatment
#19
Marcelo Palhais, Manoel Damião Sousa-Neto, Fuad Jacob Abi Rached-Junior, Mariana Carvalho de Andrade Amaral, Edson Alfredo, Carlos Eduardo Saraiva Miranda, Yara Teresinha Corrêa Silva-Sousa
This study evaluated the removal of filling material with ProTaper Universal Rotary Retreatment system (PTR) combined with solvents and the influence of solvents on the bond strength (PBS) of sealer to intraradicular dentin after canal reobturation. Roots were endodontically treated and distributed to five groups (n = 12). The control group was not retreated. In the four experimental groups, canals were retreated with PTR alone or in combination with xylol, orange oil, and eucalyptol. After filling material removal, two specimens of each group were analysed by SEM and µCT to verify the presence of filling remnants on root canal walls...
January 26, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28130130/structural-studies-of-a-vasorelaxant-lectin-from-dioclea-reflexa-hook-seeds-crystal-structure-molecular-docking-and-dynamics
#20
Vanir Reis Pinto-Junior, Vinicius José Silva Osterne, Mayara Queiroz Santiago, Jorge Luis Almeida Correia, Francisco Nascimento Pereira-Junior, Rodrigo Bainy Leal, Maria Gonçalves Pereira, Larissa Silva Chicas, Celso Shiniti Nagano, Bruno Anderson Matias Rocha, José Caetano Silva-Filho, Wandemberg Paiva Ferreira, Cíntia Renata Costa Rocha, Kyria Santiago Nascimento, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada
The three-dimensional structure of Dioclea reflexa seed lectin (DrfL) was studied in detail by a combination of X-ray crystallography, molecular docking and molecular dynamics. DrfL was purified by affinity chromatography using Sephadex G-50 matrix. Its primary structure was obtained by mass spectrometry, and crystals belonging to orthorhombic space group P212121 were grown by the vapor diffusion method at 293K. The crystal structure was solved at 1.765Å and was very similar to that of other lectins from the same subtribe...
January 24, 2017: International Journal of Biological Macromolecules
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