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https://www.readbyqxmd.com/read/28430308/arteriovenous-access-banding-revisited
#1
Gabriela Teixeira, Paulo Almeida, Clemente N Sousa, Paulo Teles, Paulo De Sousa, Luís Loureiro, Sérgio Teixeira, Duarte Rego, Rui Almeida, António Norton de Matos
PURPOSE: The aim of this study is to validate the current applicability of arteriovenous access banding in high flow access (HFA) and/or haemodialysis access-induced distal ischaemia (HAIDI). METHODS: This retrospective study was conducted at the GEV (Grupo de Estudos Vasculares) vascular access centre. The clinical records of consecutive patients undergoing banding for HAIDI and HFA symptoms, between June 2011 and January 2015, were reviewed until April 2015. All vascular access patients' consultation records and surgical notes were reviewed...
April 18, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28421792/micronutrient-supplementation-in-gastric-bypass-surgery-prospective-study-on-in%C3%AF-ammation-and-iron-metabolism-in-premenopausal-women
#2
Flávia Andréia Marin, Rozangela Verlengia, Alex Harley Crisp, Patrícia Fátima Sousa Novais, Irineu Rasera-Junior, Maria Rita Marques De Oliveira
BACKGROUND: Low-grade chronic inflammation in morbid obesity is associated with impaired iron metabolism. Bariatric surgery is effective in weight loss; however, it can induce specific nutritional deficiencies, such as iron, especially in premenopausal women. Alternatively, after surgery, there is an improvement in systemic inflammation, raising questions concerning the dosages of micronutrient supplementation. OBJECTIVES: This study aimed to assess the effect of two micronutrient supplementation schemes before and 6 months after a Roux-en-Y gastric bypass (RYGB) surgery on inflammation and iron metabolism in premenopausal women...
March 30, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28421777/quality-control-of-enteral-nutrition-therapy-in-cancer-patients-at-nutritional-risk
#3
Ariana Lee, Ronaldo Sousa Oliveira Filho, Thais De Campos Cardenas, Gislaine Aparecida Ozório, Juliana Pereira Lima Gropp, Dan Linetzky Waitzberg
INTRODUCTION: Quality indicators in nutritional therapy (QINTs) allow for the practical assessment of quality in the management of enteral nutrition therapy (ENT) among hospitalized patients. OBJECTIVE: To control ENT quality in cancer patients at nutritional risk. METHODS: A prospective, observational study was performed with cancer patients over 19 years of age who had undergone exclusive ENT for at least 72 h. Nutritional Risk Screening was used to assess nutritional risk; in the presence of nutritional risk, the Subjective Global Assessment (SGA) was used...
March 30, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28419197/inhibition-of-sarcolemmal-fat-cd36-by-sulfo-n-succinimidyl-oleate-rapidly-corrects-metabolism-and-restores-function-in-the-diabetic-heart-following-hypoxia-reoxygenation
#4
Latt S Mansor, Maria da Luz Sousa Fialho, Georgina Yea, Will A Coumans, James A West, Matthew Kerr, Carolyn A Carr, Joost J F P Luiken, Jan F C Glatz, Rhys D Evans, Julian L Griffin, Damian J Tyler, Kieran Clarke, Lisa C Heather
Aims: The type 2 diabetic heart oxidizes more fat and less glucose, which can impair metabolic flexibility and function. Increased sarcolemmal fatty acid translocase (FAT/CD36) imports more fatty acid into the diabetic myocardium, feeding increased fatty acid oxidation and elevated lipid deposition. Unlike other metabolic modulators that target mitochondrial fatty acid oxidation, we proposed that pharmacologically inhibiting fatty acid uptake, as the primary step in the pathway, would provide an alternative mechanism to rebalance metabolism and prevent lipid accumulation following hypoxic stress...
April 17, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28416319/feasibility-of-cone-beam-computed-tomography-in-detecting-lateral-canals-before-and-after-root-canal-treatment-an-ex%C3%A2-vivo-study
#5
Thiago Oliveira Sousa, Bassam Hassan, Hesam Mirmohammadi, Hagay Shemesh, Francisco Haiter-Neto
INTRODUCTION: The study objective was to evaluate the effectiveness of cone-beam computed tomographic (CBCT) imaging for the detection of lateral canals (LCs) in endodontically treated premolars. METHODS: Two evaluators classified 80 extracted premolars into 2 groups based on the absence (n = 40) or presence (n = 40) of LCs according to micro-computed tomographic analysis. The extracted teeth were fixated in a human mandible and scanned with CBCT imaging. Subsequently, each tooth was endodontically treated, and CBCT scans were repeated...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28414935/cdna-cloning-molecular-modeling-and-docking-calculations-of-l-type-lectins-from-swartzia-simplex-var-grandiflora-leguminosae-papilionoideae-a-member-of-the-tribe-swartzieae
#6
Paulo A C Maranhão, Claudener S Teixeira, Bruno L Sousa, Ito L Barroso-Neto, José E Monteiro-Júnior, Andreia V Fernandes, Marcio V Ramos, Ilka M Vasconcelos, José F C Gonçalves, Bruno A M Rocha, Valder N Freire, Thalles B Grangeiro
The genus Swartzia is a member of the tribe Swartzieae, whose genera constitute the living descendants of one of the early branches of the papilionoid legumes. Legume lectins comprise one of the main families of structurally and evolutionarily related carbohydrate-binding proteins of plant origin. However, these proteins have been poorly investigated in Swartzia and to date, only the lectin from S. laevicarpa seeds (SLL) has been purified. Moreover, no sequence information is known from lectins of any member of the tribe Swartzieae...
April 14, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28412956/management-of-suspected-common-bile-duct-stones-on-cholangiogram-during-same-stay-cholecystectomy-for-acute-gallstone-related-disease
#7
Sandra de Sousa, Olivier Tobler, Pouya Iranmanesh, Jean-Louis Frossard, Philippe Morel, Christian Toso
BACKGROUND: Recent data have suggested that upfront cholecystectomy should be performed even in the presence of moderately abnormal liver function tests (LFTs). As a consequence, more common bile duct (CBD) stones are discovered on intra-operative cholangiogram. We assessed the presentation and management of such patients to refine their management plan. METHODS: Adult patients (>16 years) with an acute gallstone-related disease who had undergone same-stay cholecystectomy from January 2013 to January 2015 were retrospectively assessed...
April 17, 2017: BMC Surgery
https://www.readbyqxmd.com/read/28412568/first-report-on-mrsa-cc398-recovered-from-wild-boars-in-the-north-of-portugal-are-we-facing-a-problem
#8
Margarida Sousa, Nuno Silva, Vera Manageiro, Sónia Ramos, António Coelho, David Gonçalves, Manuela Caniça, Carmen Torres, Gilberto Igrejas, Patrícia Poeta
The aim of the present study was to evaluate the resistance of Staphylococcus aureus recovered from wild boars, to analyze their genetic lineages, and to investigate the susceptibility to oxacillin. Samples from mouth and nose of 45 wild boars (Sus scrofa) were collected during hunt activity from November 2012 to January 2013 in the North of Portugal. S. aureus isolates were recovered from 30 of these samples (33%); one isolate/sample was further studied. The susceptibility of the isolates was tested by disk-diffusion test against 14 antimicrobial agents and minimal inhibitory concentration was used to test oxacillin according to EUCAST guidelines...
April 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28407594/trifluoromethyl-arylamides-with-antileukemia-effect-and-intracellular-inhibitory-activity-over-serine-arginine-rich-protein-kinases-srpks
#9
Raoni Pais Siqueira, Marcus Vinícius de Andrade Barros, Éverton de Almeida Alves Barbosa, Thiago Souza Onofre, Victor Hugo Sousa Gonçalves, Higor Sette Pereira, Abelardo Silva Júnior, Leandro Licursi de Oliveira, Márcia Rogéria Almeida, Juliana Lopes Rangel Fietto, Róbson Ricardo Teixeira, Gustavo Costa Bressan
The serine/arginine-rich protein kinases (SRPKs) have frequently been found with altered activity in a number of cancers, suggesting they could serve as potential therapeutic targets in oncology. Here we describe the synthesis of a series of twenty-two trifluoromethyl arylamides based on the known SRPKs inhibitor N-(2-(piperidin-1-yl)-5-(trifluoromethyl)phenyl)isonicotinamide (SRPIN340) and the evaluation of their antileukemia effects. Some derivatives presented superior cytotoxic effects against myeloid and lymphoid leukemia cell lines compared to SRPIN340...
March 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28399155/frequent-isolation-of-methicillin-resistant-staphylococcus-aureus-mrsa-st398-among-healthy-pigs-in-portugal
#10
Teresa Conceição, Hermínia de Lencastre, Marta Aires-de-Sousa
BACKGROUND: Although livestock-associated ST398 methicillin-resistant Staphylococcus aureus (MRSA) has been widely reported in different geographic regions, MRSA carriage studies among healthy pigs in Portugal are very limited. METHODS AND FINDINGS: In total, 101 swine nasal samples from two Portuguese farms were screened for MRSA. In addition five swine workers (including one veterinary and one engineer) and four household members were nasally screened. The isolates were characterized by spa typing, SCCmec typing and MLST...
2017: PloS One
https://www.readbyqxmd.com/read/28398741/adductomic-screening-of-hemoglobin-adducts-and-monitoring-of-micronuclei-in-school-age-children
#11
Henrik Carlsson, Jenny Aasa, Natalia Kotova, Daniel Vare, Pedro F M Sousa, Per Mikael Rydberg, Lilianne Abramsson-Zetterberg, Margareta Åsa Törnqvist
Electrophilic compounds/metabolites present in humans, originating from endogenous processes or exogenous exposure, pose a risk for health effects through their reactions with nucleophilic sites in proteins and DNA, forming adducts. Adductomic approaches are developed to screen for adducts to biomacromolecules in vivo by mass spectrometry (MS), with the aim to detect adducts corresponding to unknown exposures from electrophiles. In the present study, adductomic screening was performed using blood samples from healthy children about 12 years old (n=51)...
April 11, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28397837/the-nucleus-reuniens-a-key-node-in-the-neurocircuitry-of-stress-and-depression
#12
V Kafetzopoulos, N Kokras, I Sotiropoulos, J F Oliveira, H Leite-Almeida, A Vasalou, V M Sardinha, Z Papadopoulou-Daifoti, O F X Almeida, K Antoniou, N Sousa, C Dalla
The hippocampus and prefrontal cortex (PFC) are connected in a reciprocal manner: whereas the hippocampus projects directly to the PFC, a polysynaptic pathway that passes through the nucleus reuniens (RE) of the thalamus relays inputs from the PFC to the hippocampus. The present study demonstrates that lesioning and/or inactivation of the RE reduces coherence in the PFC-hippocampal pathway, provokes an antidepressant-like behavioral response in the forced swim test and prevents, but does not ameliorate, anhedonia in the chronic mild stress (CMS) model of depression...
April 11, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28396017/cell-surface-targeting-of-the-melanocortin-5-receptor-mc5r-requires-serine-rich-terminal-motifs
#13
A R Rodrigues, D Sousa, H Almeida, A M Gouveia
The Melanocortin 5 Receptor (MC5R) is a cell surface receptor that belongs to the class of G-protein coupled receptors (GPCRs), which comprises an intracellular carboxylic domain, seven transmembrane helices and an extracellular amino terminal. Over the last few years, MC5R has been implicated in the regulation of lipid metabolism in exocrine glands, muscle and even in adipose tissue and its function is quite dependent on its correct cell membrane targeting. In this context, the purpose of this work was to study the role of MC5R N-terminus in the receptor trafficking from the Endoplasmic Reticulum (ER) through the Golgi complex to the plasma membrane...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28385296/17%C3%AE-estradiol-prevents-mesenteric-injury-induced-by-occlusion-of-the-proximal-descending-aorta-in-male-rats
#14
Paulo Thales Rocha de Sousa, Ana Cristina Breithaupt-Faloppa, Cristiano de Jesus Correia, Raif Restivo Simão, Sueli Gomes Ferreira, Alfredo Inácio Fiorelli, Luiz Felipe Pinho Moreira, Paulina Sannomiya
OBJECTIVE: In surgical aortic repair or cardiac surgery with aorta occlusion, the occurrence of mesenteric ischemia and bowel injury has been associated with higher short-term mortality. The vascular protection of estrogens has been investigated and is mainly mediated by increasing the availability of nitric oxide (NO). Therefore, this study investigated the role of 17β-estradiol on visceral ischemia-reperfusion (I/R) injury after descending aorta occlusion in male rats. METHODS: Mesenteric ischemia was induced in male Wistar rats by placing a 2F Fogarty arterial embolectomy catheter (Edwards Lifesciences, Irvine, Calif) in the descending aorta, which remained occluded for 15 minutes, followed by reperfusion for up to 2 hours...
April 3, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28382474/selection-and-characterization-of-coal-mine-autochthonous-rhizobia-for-the-inoculation-of-herbaceous-legumes
#15
Anabel González Hernández, Ginaini Doin de Moura, Renato Leal Binati, Francisco Xavier Inês Nascimento, Diana Morales Londoño, Ana Carolina Peixoto Mamede, Emanuela Pille da Silva, Rafael Dutra de Armas, Admir José Giachini, Márcio José Rossi, Cláudio Roberto Fonsêca Sousa Soares
Coal open pit mining in the South of Santa Catarina state (Brazil) was inappropriately developed, affecting approximately 6.700 ha. Re-vegetation is an alternative for the recovery of these areas. Furthermore, the use of herbaceous legumes inoculated with nitrogen fixing bacteria is motivated due to the difficulty implementing a vegetation cover in these areas, mainly due to low nutrient availability. Therefore, the aim of this work was to evaluate, among 16 autochthonous rhizobia isolated from the coal mining areas, those with the greatest potential to increase growth of the herbaceous legumes Vicia sativa and Calopogonium mucunoides...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28380332/partial-characterization-and-immobilization-in-cnbr-activated-sepharose-of-a-native-lectin-from-platypodium-elegans-seeds-pela-and-comparative-study-of-edematogenic-effect-with-the-recombinant-form
#16
David Alencar Araripe, Vanir Reis Pinto-Junior, Antonio Hadson Bastos Neco, Mayara Queiroz Santiago, Vinicius Jose Silva Osterne, Alana Freitas Pires, Claudia Figueiredo Lossio, Maria Gleiciane Queiroz Martins, Jorge Luiz Almeida Correia, Raquel Guimaraes Benevides, Rodrigo Bainy Leal, Ana Maria Sampaio Assreuy, Kyria Santiago Nascimento, Benildo Sousa Cavada
The lectin from Platypodium elegans seeds (PELa) was purified by affinity chromatography in a mannose-agarose column. The lectin agglutinated rabbit erythrocytes and the agglutinating effect was inhibited by previous incubation with the glycoprotein fetuin, along with N-acetyl-d-glucosamine, D-mannose and its derivatives. The lectin maintained complete activity in temperatures ranging from 40 to 60°C and pH values ranging from 9 to 10. As a glycoprotein, PELa has a carbohydrate content of 2.2%, and its activity requires divalent cations such as Ca(2+) and Mn(2+)...
April 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28375967/rectus-femoris-transfer-surgery-worsens-crouch-gait-in-children-with-cerebral-palsy-at-gmfcs-levels-iii-and-iv
#17
Ted C Sousa, Alexander Nazareth, Susan A Rethlefsen, Nicole M Mueske, Tishya A L Wren, Robert M Kay
BACKGROUND: Previous study has shown that children with cerebral palsy (CP) functioning at Gross Motor Function Classification System (GMFCS) levels III and IV do not benefit from distal rectus femoris transfer (DRFT) due to lack of improvement in stance knee extension. The fate of knees in such subjects who do not undergo DRFT is unknown. The purpose of this study was to compare knee kinematic outcomes in patients with CP and stiff knee gait who underwent single-event multilevel surgery with and without DRFT...
April 3, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28371333/behavior-of-prostate-cancer-cells-in-a-nanohydroxyapatite-collagen-bone-scaffold
#18
S Cruz-Neves, N Ribeiro, I Graça, C Jerónimo, S R Sousa, F J Monteiro
Prostate cancer (PCa) is the second leading cause of death among men in Europe and US. The metastatic dissemination pattern of PCa is unique, developing bone metastasis as the only site of progression, consequently with a prognosis very poor. The cancer cells interactions within the surrounding bone environment are critical for tumor growth and progression. SPARC (Secreted Protein, Acidic and Rich in Cysteine) is described to be involved in PCa cells migration and invasion into bone. 3D in vitro systems that are able to closely resemble the in vivo microenvironment are recently taking importance in cancer research...
March 28, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28370221/molecular-analysis-of-norovirus-in-specimens-from-children-enrolled-in-a-1982-1986-study-in-bel%C3%A3-m-brazil-a-community-based-longitudinal-study
#19
Jones Anderson Monteiro Siqueira, Edivaldo Costa Sousa Júnior, Alexandre da Costa Linhares, Yvone Benchimol Gabbay
Fecal specimens were collected during a longitudinal, community-based study in the city of Belém, North Brazil, that was conducted over 3 years (October 1982 to March 1986), in which 20 children were included from birth to 3 years of age. A total of 229 fecal samples were screened by real time RT-PCR targeting the junction region (ORF 1/2) of the norovirus (NoV) genome. NoV-positive samples were subjected to PCR and sequencing of the viral polymerase (ORF1) and viral protein 1 (VP1) genes (ORF2). The junction region was also sequenced to assess for recombination when ORF1 and ORF2 genotyping results were dissimilar...
March 29, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28366511/occurrence-and-molecular-characterization-of-hemoplasmas-in-domestic-dogs-and-wild-mammals-in-a-brazilian-wetland
#20
Keyla Carstens Marques de Sousa, Heitor Miraglia Herrera, Caroline Tostes Secato, André do Vale Oliveira, Filipe Martins Santos, Fabiana Lopes Rocha, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho Macedo, Pedro Cordeiro Estrela de Andrade Pinto, Rosangela Zacarias Machado, Mirela Tinucci Costa, Marcos Rogério André
Hemotropic mycoplasmas are known to cause anemia in several mammalian species. The present work aimed to investigate the occurrence of Mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil. Between August 2013 and March 2015, 31 Nasua nasua, 78 Cerdocyon thous, seven Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were collected. Mammals and ectoparasites DNA samples were submitted to conventional PCR assays for Mycoplasma spp...
March 31, 2017: Acta Tropica
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