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burkholderia contaminans

Peng Deng, Adam Foxfire, Jianhong Xu, Sonya M Baird, Jiayuan Jia, Keren H Delgado, Ronald Shin, Leif Smith, Shi-En Lu
Burkholderia contaminans MS14 was isolated from soil in Mississippi. When cultivated on nutrient broth-yeast extract (NBY) agar medium, the colonies exhibit bactericidal activity against a wide range of plant pathogenic bacteria. Only a bacteriostatic compound with siderophore activity was successfully purified, which was determined by nuclear magnetic resonance (NMR) to be ornibactin. The isolation of the bactericidal compound has not yet been achieved, thus, the exact nature of the bactericidal compound is still unknown...
February 10, 2017: Applied and Environmental Microbiology
Burke A Cunha, John Gian, Bertamaria Dieguez, Elsa Santos-Cruz, Daniela Matassa, Steve Gerson, Pat Daniels, Carlos Rosales, Rodger P Silletti
Objective:B. contaminans was cultured from respiratory secretions and liquid docusate (Colace) in a Neurosurgical Intensive Care Unit (NICU) patient with community-acquired Legionnaire's disease but not from another bottle given to the patient. Unexpectedly, C. pelliculosa was cultured from two bottles, but not the B. contaminans bottle or respiratory secretions. Methods:B. cepacia, later identified as B. contaminans, was cultured from a bottle of liquid docusate (Colace) dispensed to a non-cystic fibrosis patient...
November 30, 2016: Journal of Clinical Medicine
Maria Sol Haim, Marta Mollerach, Gary Van Domselaar, Sergio A Teves, José Degrossi, Silvia T Cardona
Burkholderia contaminans is a species of the Burkholderia cepacia complex, a group of bacteria that can grow in pharmaceutical products and are capable of infecting the immunocompromised and people with cystic fibrosis. Here, we report draft genome sequences for Burkholderia contaminans FFI-28, a strain isolated from a contaminated pharmaceutical solution.
October 27, 2016: Genome Announcements
Jaroslav Nunvar, Lucie Kalferstova, Ruhi A M Bloodworth, Michal Kolar, Jose Degrossi, Silvina Lubovich, Silvia T Cardona, Pavel Drevinek
Several bacterial species from the Burkholderia cepacia complex (Bcc) are feared opportunistic pathogens that lead to debilitating lung infections with a high risk of developing fatal septicemia in cystic fibrosis (CF) patients. However, the pathogenic potential of other Bcc species is yet unknown. To elucidate clinical relevance of Burkholderia contaminans, a species frequently isolated from CF respiratory samples in Ibero-American countries, we aimed to identify its key virulence factors possibly linked with an unfavorable clinical outcome...
2016: PloS One
Jae-Hoon Choi, Ayaka Kikuchi, Panyapon Pumkaeo, Hirofumi Hirai, Shinji Tokuyama, Hirokazu Kawagishi
Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. We previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH). In this study, a bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX. The strain converted AHX to AOH and identified as Burkholderia contaminans based on the gene sequence of its 16S rDNA. The quantitative production of AOH by resting cells of the strain was achieved...
October 2016: Bioscience, Biotechnology, and Biochemistry
Rachel F Power, Barry Linnane, Ruth Martin, Noelle Power, Peig Harnett, Brian Casserly, Nuala H O'Connell, Colum P Dunne
BACKGROUND: Burkholderia contaminans is an emerging pathogen in the cystic fibrosis (CF) setting. Included in the Burkholderia cepacia complex (Bcc), B. contaminans is a Gram negative, motile, obligate aerobe previously classified as a pseudomonad. Previous reports have described B. contaminans isolation from patients in Portugal, Switzerland, Spain, Argentina and the USA. This, however, is the first report relating to B. contaminans affecting Irish patients with CF, initially detected in a paediatric setting...
April 22, 2016: BMC Pulmonary Medicine
João M S Cardoso, Adelino M Galvão, Soraia I Guerreiro, Jorge H Leitão, Ana C Suarez, M Fernanda N N Carvalho
Five new silver camphorimine complexes of general formula [Ag(NO3)(Y)L] were synthesized and fully characterized using spectroscopic and analytical techniques. The structure of [Ag(NO3)(OC10H14NC6H4NC10H14O)] () was analyzed using single crystal X-ray diffraction, showing that it arranges as a coordination polymer formed by sequential Ag(NO3) units bridged by the bi-camphor ligand (). The antimicrobial properties of the new complexes were screened using the disk diffusion method and their Minimal Inhibitory Concentrations (MIC) were assessed against selected bacterial strains of the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli, Pseudomonas aeruginosa, and Burkholderia contaminans...
April 28, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
X Q Wang, A X Liu, A Guerrero, J Liu, X Q Yu, P Deng, L Ma, S M Baird, L Smith, X D Li, S E Lu
AIMS: To identify the taxonomy of tobacco rhizosphere-isolated strain Lyc2 and investigate the mechanisms of the antifungal activities, focusing on antimicrobials gene clusters identification and function analysis. METHODS AND RESULTS: Multilocus sequence typing and 16S rRNA analyses indicated that strain Lyc2 belongs to Burkholderia pyrrocinia. Bioassay results indicated strain Lyc2 showed significant antifungal activities against a broad range of plant and animal fungal pathogens and control efficacy on seedling damping off disease of cotton...
March 2016: Journal of Applied Microbiology
Ricardo Araujo, Rita Caramalho, Carla Coutinho, Isabel Sá-Correia
We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans. Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.
February 2016: Journal of Clinical Microbiology
Carina M Hall, Joseph D Busch, Kenzie Shippy, Christopher J Allender, Mirjam Kaestli, Mark Mayo, Jason W Sahl, James M Schupp, Rebecca E Colman, Paul Keim, Bart J Currie, David M Wagner
The global distribution of the soil-dwelling bacterium Burkholderia pseudomallei, causative agent of melioidosis, is poorly understood. We used established culturing methods developed for B. pseudomallei to isolate Burkholderia species from soil collected at 18 sampling sites in three states in the southern United States (Arizona (n = 4), Florida (n = 7), and Louisiana (n = 7)). Using multi-locus sequence typing (MLST) of seven genes, we identified 35 Burkholderia isolates from these soil samples. All species belonged to the B...
2015: PloS One
Esteban C Nannini, Adriana Ponessa, Rosa Muratori, Patricia Marchiaro, Viviana Ballerini, Luis Flynn, Adriana S Limansky
A nosocomial polyclonal outbreak associated to bacteremia caused by different Burkholderia cepacia complex (BCC) species and clones is reported. Molecular characterization identified Burkholderia stabilis, Burkholderia contaminans, and Burkholderia ambifaria among BCC isolates obtained from patients in neonatal and adult intensive care units. BCC was also isolated from an intrinsically contaminated ultrasound gel, which constituted the presumptive BCC source. Prior BCC outbreak related to contaminated ultrasound gels have been described in the setting of transrectal prostate biopsy...
September 2015: Brazilian Journal of Infectious Diseases
Ruhi A M Bloodworth, Carrie Selin, Maria Agustina López De Volder, Pavel Drevinek, Laura Galanternik, José Degrossi, Silvia T Cardona
Burkholderia contaminans belongs to the Burkholderia cepacia complex (BCC), a group of bacteria that are ubiquitous in the environment and capable of infecting the immunocompromised and people with cystic fibrosis. We report here draft genome sequences for the B. contaminans type strain LMG 23361 and an Argentinian cystic fibrosis sputum isolate.
2015: Genome Announcements
Carla P Coutinho, Celeste Barreto, Luísa Pereira, Luís Lito, José Melo Cristino, Isabel Sá-Correia
The Burkholderia cepacia complex (Bcc) is a heterogeneous group of bacteria comprising around 20 related species. These bacteria are important opportunistic pathogens, especially in cystic fibrosis (CF) patients, and are associated with a worse prognosis and decreased life expectancy. The taxonomic position of 20 Bcc isolates retrieved from CF patients receiving care at Hospital Santa Maria (HSM), in Lisbon, from 1995 to 2006, was re-examined in the present work. These isolates, formerly classified as Burkholderia cepacia (taxon K), are here reclassified as Burkholderia contaminans, including the former B...
August 2015: Journal of Medical Microbiology
Agnese Lupo, Enver Isis, Regula Tinguely, Andrea Endimiani
For the first time, we analyzed the clonality and susceptibility of Burkholderia cepacia complex isolates (n=55) collected during 1998-2013 from 44 Swiss cystic fibrosis (CF)-patients. B. cenocepacia (n=28) and B. multivorans (n=14) were mainly of sequence type (ST) 833 and ST874, respectively; B. contaminans isolates were of ST102. Overall, the following MIC50/90s (mg/l) were obtained: piperacillin/tazobactam (≤ 4/≥ 128), ticarcillin/clavulanate (≥ 256/≥256), ceftazidime (2/≥ 32), aztreonam (16/≥ 32), meropenem (2/8), tobramycin (8/≥ 16), minocycline (≤ 1/16), levofloxacin (≤ 0...
April 2015: New Microbiologica
M J Medina-Pascual, S Valdezate, G Carrasco, P Villalón, N Garrido, J A Saéz-Nieto
Species of the Burkholderia cepacia complex are associated with opportunistic infection in patients with cystic fibrosis. For years now, B. multivorans and B. cenocepacia have been the most frequently isolated species within the complex in such patients. However, between 2008 and 2012, the overall incidence of these species in Spain (17.7% and 12.5% respectively) was overtaken by that of B. contaminans (36.5%). The population structure of B. contaminans isolates from Spanish patients with cystic fibrosis was analysed using multilocus sequence typing and pulsed-field gel electrophoresis (PFGE)...
February 2015: Clinical Microbiology and Infection
Ana S Ferreira, Inês N Silva, Fábio Fernandes, Ruth Pilkington, Máire Callaghan, Siobhán McClean, Leonilde M Moreira
Bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, their roles in the stress response, DNA metabolism, cell division, and virulence have also been documented. The aim of this study was to investigate the pathogenicity and potential mechanisms of virulence dependent on the tyrosine kinase BceF and phosphotyrosine phosphatase BceD of the cystic fibrosis opportunistic pathogen Burkholderia contaminans IST408. The insertion mutants bceD::Tp and bceF::Tp showed similar attenuation of adhesion and invasion of the cystic fibrosis lung epithelial cell line CFBE41o- compared to the parental strain B...
February 2015: Infection and Immunity
Pablo Martina, Sofía Feliziani, Carlos Juan, Marisa Bettiol, Blanca Gatti, Osvaldo Yantorno, Andrea M Smania, Antonio Oliver, Alejandra Bosch
The Burkholderia cepacia complex (Bcc) represents an important group of pathogens involved in long-term lung infection in cystic fibrosis (CF) patients. A positive selection of hypermutators, linked to antimicrobial resistance development, has been previously reported for Pseudomonas aeruginosa in this chronic infection setting. Hypermutability, however, has not yet been systematically evaluated in Bcc species. A total of 125 well characterized Bcc isolates recovered from 48 CF patients, 10 non-CF patients and 15 environmental samples were analyzed...
November 2014: International Journal of Medical Microbiology: IJMM
Rebekah W Moehring, Sarah S Lewis, Pamela J Isaacs, Wiley A Schell, Wayne R Thomann, Mary M Althaus, Kevin C Hazen, Kristen V Dicks, John J Lipuma, Luke F Chen, Daniel J Sexton
IMPORTANCE Many health care facilities compound medications on site to fulfill local demands when customized formulations are needed, national supply is critically low, or costs for manufactured pharmaceuticals are excessive. Small, institutional compounding facilities may perform the same high-risk procedures as large distributors of compounded medications. OBJECTIVES To investigate an outbreak related to contamination of compounded sterile preparations and to determine processes to prevent future outbreaks...
April 2014: JAMA Internal Medicine
Fernanda Matias, Maria Filomena de Andrade Rodrigues
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources...
October 2011: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
A L Voronina, M Yu Chernukha, I A Shaginyan, M S Kunda, L R Avetisyan, A A Orlova, V G Lunin, L V Avakyan, N I Kapranov, E L Amelina, A G Chuchalin, Gintsburg A L
88 cultures of microorganisms referred to the Burkholderia cepacia complex (Bcc) during initial identification were analyzed by multilocus sequencing (Multilocus Sequence Typing, MLST). 13 genotypes (sequence type, ST) were detected, 9 of them (708, 709, 710, 711, 712, 714, 727, 728, 729) were identified for the first time. Two new alleles for the gene trpB (357, 358), one of the genes atpD (306) and gltB (352) were detected and registered. It was found that strains of 2 genotypes (711, 712) belong to the species B...
2013: Molekuliarnaia Genetika, Mikrobiologiia i Virusologiia
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