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https://www.readbyqxmd.com/read/17243152/lif-immobilized-nonwoven-polyester-fabrics-for-cultivation-of-murine-embryonic-stem-cells
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Gaye Cetinkaya, Hilal Türkoğlu, Sezen Arat, Hande Odaman, Mehmet A Onur, Menemşe Gümüşderelioğlu, Aşkin Tümer
Embryonic stem (ES) cells have a great interest for tissue engineering because of their pluripotent nature and proliferative capacity. The objective of this study is to constitute a synthetic microenvironment to support the in vitro propagation of murine ES cells in an undifferentiated state. That is why we used a three-dimensional matrix, nonwoven polyester fabric (NWPF), which was formed from poly(ethylene terephthalate) (PET) fibers. NWPF discs were partially hydrolyzed, and then the carboxyl groups were coupled with leukemia inhibitory factor (LIF) in the presence of water-soluble carbodiimide...
June 15, 2007: Journal of Biomedical Materials Research. Part A
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