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Lin28A

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https://www.readbyqxmd.com/read/29775782/network-based-expression-analyses-and-experimental-validations-revealed-high-co-expression-between-yap1-and-stem-cell-markers-compared-to-differentiated-cells
#1
Fariba Dehghanian, Zohreh Hojati, Fariba Esmaeili, Ali Masoudi-Nejad
The Hippo signaling pathway is identified as a potential regulatory pathway which plays critical roles in differentiation and stem cell self-renewal. Yap1 is a primary transcriptional effector of this pathway. The importance of Yap1 in embryonic stem cells (ESCs) and differentiation procedure remains a challenging question, since two different observations have been reported. To answer this question we used co-expression network and differential co-expression analyses followed by experimental validations. Our results indicate that Yap1 is highly co-expressed with stem cell markers in ESCs but not in differentiated cells (DCs)...
May 15, 2018: Genomics
https://www.readbyqxmd.com/read/29758178/lin28a-regulates-neurogliogenesis-in-mammalian-retina-through-the-igf-signaling
#2
Xiaohuan Xia, Pooja Teotia, Iqbal Ahmad
In the developing central nervous system (CNS) the majority of neurons are born before the generation of glia. Emerging evidence implicates heterochronic gene, Lin28 in the temporal switch between two distinct lineages. However, the respective contributions of Lin28a and Lin28b in neurogliogenesis remain poorly understood. Here, we have examined the relative involvement of Lin28a and Lin28b in mammalian retina, a simple and accessible CNS model where neurogliogenic decision largely occurs postnatally. Examination of Lin28a/b involvement during late histogenesis by the perturbation of function approaches revealed that while Lin28b did not influence differentiation in general Lin28a facilitated and antagonized the generation of neurons and glia, respectively...
May 11, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29749495/lin28a-inhibits-lysosome%C3%A2-associated-membrane-glycoprotein-1-protein-expression-in-embryonic-stem-and-bladder-cancer-cells
#3
Peng Pan, Ting Chen, Yanmin Zhang, Zhengyu Qi, Jie Qin, Guanghui Cui, Xin Guo
Tumor cells and embryonic stem cells (ESCs) have similar transcription mechanisms. LIN28A is an important factor in tumor cells and ESCs, it is an inhibitor of intracellular endoplasmic reticulum (ER)‑related protein translation in ESCs. The present study aimed to examine the effects of LIN28A on an ER‑related protein, lysosome‑associated membrane glycoprotein 1 (LAMP1), in human bladder cancer cells and mouse (m)ESCs, using reverse transcription‑quantitative polymerase chain reaction and western blotting to detect the expression of LAMP1 mRNA and protein, respectively, following LIN28A knockdown...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29742265/antiproliferative-effect-of-urolithin-a-the-ellagic-acid-derived-colonic-metabolite-on-hepatocellular-carcinoma-hepg2-2-15-cells-by-targeting-lin28a-let-7a-axis
#4
Zhenpeng Qiu, Junxuan Zhou, Cong Zhang, Ye Cheng, Junjie Hu, Guohua Zheng
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29719254/let-7-microrna-mediated-regulation-of-shh-signaling-and-the-gene-regulatory-network-is-essential-for-retina-regeneration
#5
Simran Kaur, Shivangi Gupta, Mansi Chaudhary, Mohammad Anwar Khursheed, Soumitra Mitra, Akshai Janardhana Kurup, Rajesh Ramachandran
Upon injury, Müller glia cells of the zebrafish retina reprogram themselves to progenitor cells with stem cell characteristics. This necessity for retina regeneration is often compromised in mammals. We explored the significance of developmentally inevitable Sonic hedgehog signaling and found its necessity in MG reprogramming during retina regeneration. We report on stringent translational regulation of sonic hedgehog, smoothened, and patched1 by let-7 microRNA, which is regulated by Lin28a, in Müller glia (MG)-derived progenitor cells (MGPCs)...
May 1, 2018: Cell Reports
https://www.readbyqxmd.com/read/29674645/long-noncoding-rna-tug1-facilitates-osteogenic-differentiation-of-periodontal-ligament-stem-cells-via-interacting-with-lin28a
#6
Qin He, Shuangyan Yang, Xiuge Gu, Mengying Li, Chunling Wang, Fulan Wei
Periodontal ligament stem cells (PDLSCs) are mesenchymal stem cells derived from dental tissues with multidirectional differentiation potential and excellent self-renewing ability. Recently, long noncoding RNAs (lncRNAs) have been shown to play important roles in MSC osteogenic differentiation. In this study, we found that taurine upregulated gene 1 (TUG1), an evolutionarily conserved and widely present lncRNA was significantly upregulated in osteogenically induced PDLSCs compared to their undifferentiated counterparts...
April 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29577687/-mettl3-regulates-expression-of-pluripotent-genes-in-porcine-pluripotent-stem-cells
#7
Ziyu Ma, Yahui Ren, Min Ling, Huayan Wang
In post-transcriptional mRNA modification, m⁶A has been observed in a wide range of eukaryotes. METTL3, as a component of methyltransferase complex for m⁶A modification, regulates mouse naïve pluripotency and influences mRNA stability, especially affecting the expression level of the key pluripotent transcription factors. To reveal the expression pattern of the porcine METTL3 gene, we analyzed METTL3 expression level in different porcine tissues, somatic cells, and induced pluripotent stem cells (piPSCs) by RT-PCR...
March 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29552298/synergistic-neuroprotective-effect-of-rasagiline-and-idebenone-against-retinal-ischemia-reperfusion-injury-via-the-lin28-let-7-dicer-pathway
#8
Dawei Lei, Zhengbo Shao, Xinrong Zhou, Huiping Yuan
Retinal ischemia-reperfusion (RIR) injury causes neuronal degeneration and initiates various optic nerve diseases. This study aimed to investigate the synergistic neuroprotective effect of rasagiline and idebenone against RIR injury. A combination of rasagiline and idebenone was administered intraperitoneally immediately after establishment of the RIR model. Treatment with the combination of the two drugs resulted in a significant restoration of retinal thickness and retinal ganglion cells. Apoptosis of cells in ganglion cell layers was also ameliorated, suggesting that the effect of the two drugs was synergistic and the expression of brain-derived neurotrophic factor increased...
February 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29540527/a-biosensor-for-mapk-dependent-lin28-signaling
#9
Laurel M Oldach, Kirill Gorshkov, William T Mills, Jin Zhang, Mollie K Meffert
Intracellular levels of the RNA-binding protein and pluripotency factor, Lin28a, are tightly controlled to govern cellular and organismal growth. Lin28a is extensively regulated at the posttranscriptional level, and can undergo mitogen-activated protein kinase (MAPK)-mediated elevation from low basal levels in differentiated cells by phosphorylation-dependent stabilizing interaction with the RNA-silencing factor HIV TAR RNA-binding protein (TRBP). However, molecular and spatiotemporal details of this critical control mechanism remained unknown...
May 15, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29462197/thymic-epithelial-cell-derived-signals-control-b-progenitor-formation-and-proliferation-in-the-thymus-by-regulating-let-7-and-arid3a
#10
Shiyun Xiao, Wen Zhang, Nancy R Manley
The postnatal thymus is an efficient microenvironment for T cell specification and differentiation. B cells are also present in the thymus and have been recently shown to impact T cell selection, however, the mechanisms controlling B cell development in the thymus are largely unknown. In Foxn1lacZ mutant mice, down-regulation of Foxn1 expression in thymic epithelial cells beginning 1 week after birth caused a dramatic reduction of T progenitors and an increase of B cell progenitors. This time point is coincident with the switch from fetal to adult-type hematopoietic stem cells (HSCs), which is regulated by the Lin28-Let7 system...
2018: PloS One
https://www.readbyqxmd.com/read/29458410/human-regulatory-proteins-associate-with-non-coding-rnas-from-the-ebv-ir1-region
#11
V S Tompkins, D P Valverde, W N Moss
OBJECTIVE: The function of Epstein-Barr virus (EBV) stable intronic sequence (sis)RNAs, non-coding RNAs transcribed from a region required for EBV-mediated cellular transformation, remain unknown. To better understand the function of ebv-sisRNA-1 and ebv-sisRNA-2 from the internal repeat (IR)1 region of EBV, we used a combination of bioinformatics and biochemistry to identify associated RNA binding proteins. The findings reported here are part of ongoing studies to determine the functions of non-coding RNAs from the IR1 region of EBV...
February 20, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29378863/notch-suppression-collaborates-with-ascl1-and-lin28-to-unleash-a-regenerative-response-in-fish-retina-but-not-in-mice
#12
Fairouz Elsaeidi, Peter Macpherson, Elizabeth A Mills, Jonathan Jui, John G Flannery, Daniel Goldman
Müller glial (MG) cells in the zebrafish retina respond to injury by acquiring retinal stem-cell characteristics. Thousands of gene expression changes are associated with this event. Key among these changes is the induction of Ascl1a and Lin28a, two reprogramming factors whose expression is necessary for retina regeneration. Whether these factors are sufficient to drive MG proliferation and subsequent neuronal-fate specification remains unknown. To test this, we conditionally expressed Ascl1a and Lin28a in the uninjured retina of male and female fish...
February 28, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29337138/let-7-suppresses-b-cell-activation-through-restricting-the-availability-of-necessary-nutrients
#13
Shuai Jiang, Wei Yan, Shizhen Emily Wang, David Baltimore
The control of uptake and utilization of necessary extracellular nutrients-glucose and glutamine-is an important aspect of B cell activation. Let-7 is a family of microRNAs known to be involved in metabolic control. Here, we employed several engineered mouse models, including B cell-specific overexpression of Lin28a or the let-7a-1/let-7d/let-7f-1 cluster (let-7adf) and knockout of individual let-7 clusters to show that let-7adf specifically inhibits T cell-independent (TI) antigen-induced immunoglobulin (Ig)M antibody production...
February 6, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29301498/pcaf-mediated-acetylation-of-lin28b-increases-let-7-biogenesis-in-lung-adenocarcinoma-h1299-cells
#14
Ting-Ting Qu, Fei Chen, Jing Wang, Yan-Jun Zhang, Mo-Bin Cheng, Wen-Zheng Sun, Yu-Fei Shen, Ye Zhang
BACKGROUND: Lin28B and its paralog Lin28A are small RNA binding proteins that have similar inhibitory effects, although they target separate steps in the maturation of let-7 miRNAs in mammalian cells. Because Lin28B participates in the promotion and development of tumors mostly by blocking the let-7 tumor suppressor family members, we sought to explore the associated mechanisms to gain insights into how Lin28B might be decreased in human cancer cells to increase let-7 levels and reverse malignancy...
January 4, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29245067/lin28a-functionally-modulates-bupivacaine-induced-dorsal-root-ganglion-neuron-apoptosis-through-trka-activation
#15
Liangsheng Yu, Yuanxu Jiang, Bin Tang
PURPOSE: Lin-28 Homolog A gene (Lin28a) is an important regulator in nerve system. In this study, we investigated the functional mechanism of Lin28a during the process of bupivacaine (BUP)-induced neuronal apoptosis of spinal cord dorsal root ganglion neurons (DRGNs). METHODS: Young mouse DRGNs were cultured in vitro and treated with series concentrations of BUP. Apoptosis was evaluated by TUNEL assay. Corresponding Lin28a mRNA and protein expressions were evaluated by qRT-PCR and western blot (WB) assays...
February 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29204769/rna-binding-protein-lin28a-promotes-osteocarcinoma-cells-progression-by-associating-with-the-long-noncoding-rna-malat1
#16
Zhen Wang, Jinhui Pang, Bin Ji, Shailin Zhang, Yan Cheng, Luchao Yu, Weicheng Pan
OBJECTIVES: To explore the effects of Lin28A on progression of osteocarcinoma (OS) cells. RESULTS: Lin28A mRNA and protein expressions were significantly increased in OS tissues compared with that in normal adjacent tissues. Expressions of Lin28A and long noncoding RNA MALAT1 were positively correlated. Patients with higher Lin28A expression had shorter overall survival. Moreover, Lin28A knockdown inhibited OS cells proliferation, migration, invasion and promoted cell apoptosis; Lin28A was found to harbor binding sites on MALAT1 sequences and associated with MALAT1, and increased MALAT1 stability and expression...
March 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29143345/lin28a-enhances-in-vitro-osteoblastic-differentiation-of-human-periosteum-derived-cells
#17
Jin-Ho Park, Bong-Wook Park, Young-Hoon Kang, Sung-Hoon Byun, Sun-Chul Hwang, Deok Ryong Kim, Dong Kyun Woo, June-Ho Byun
Despite a capacity for proliferation and an ability to differentiate into multiple cell types, in long-term culture and with ageing, stem cells show a reduction in growth, display a decrease in differentiation potential, and enter senescence without evidence of transformation. The Lin28a gene encodes an RNA-binding protein that plays a role in regulating stem cell activity, including self-renewal and differentiation propensity. However, the effect of the Lin28a gene on cultured human osteoprecursor cells is poorly understood...
December 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29072697/requirement-for-ccnb1-in-mouse-spermatogenesis
#18
Ji-Xin Tang, Jian Li, Jin-Mei Cheng, Bian Hu, Tie-Cheng Sun, Xiao-Yu Li, Aalia Batool, Zhi-Peng Wang, Xiu-Xia Wang, Shou-Long Deng, Yan Zhang, Su-Ren Chen, Xingxu Huang, Yi-Xun Liu
Spermatogenesis, which involves mitosis and meiosis of male germ cells, is a highly complicated and coordinately ordered process. Cyclin B1 (CCNB1), an important regulator in cell cycle machinery, is proved essential for mouse embryonic development. However, the role of CCNB1 in mammalian spermatogenesis remains unclear. Here we tested the requirement for CCNB1 using conditional knockout mice lacking CCNB1 in male germ cells. We found that ablation of CCNB1 in gonocytes and spermatogonia led to mouse sterile caused by the male germ cells' depletion...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29066347/jak2v617f-influences-epigenomic-changes-in-myeloproliferative-neoplasms
#19
Chih-Cheng Chen, Chia-Chen Chiu, Kuan-Der Lee, Chia-Chen Hsu, Hong-Chi Chen, Tim H-M Huang, Shu-Huei Hsiao, Yu-Wei Leu
Negative valine (V) to phenylalanine (F) switch at the Janus kinase (JAK2) 617 codon (V617F) is the dominant driver mutation in patients with myeloproliferative neoplasms (MPNs). JAK2V617F was proved to be sufficient for cell transformation; however, independent mutations might influence the following epigenomic modifications. To assess the JAK2V617F-induced downstream epigenomic changes without interferences, we profiled the epigenomic changes in ectopically expressed JAK2V617F in Ba/F3 cells. Antibodies against phosphorylated signal transducer and activator of transcription 3 (pSTAT3) and enhancer of zeste homolog 2 (EZH2) were used for chromatin-immunoprecipitation sequencing (ChIP-seq) to detect the downstream epigenomic targets in the JAK2-STAT3 signaling pathway...
December 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29048434/common-microrna-mrna-interactions-exist-among-distinct-porcine-ipsc-lines-independent-of-their-metastable-pluripotent-states
#20
Shiqiang Zhang, Youlong Xie, Hongxia Cao, Huayan Wang
Previous evidences have proved that porcine-induced pluripotent stem cells (piPSCs) could be induced to distinctive metastable pluripotent states. This raises the issue of whether there is a common transcriptomic profile existing among the piPSC lines at distinctive state. In this study, we performed conjoint analysis of small RNA-seq and mRNA-seq for three piPSC lines which represent LIF dependence, FGF2 dependence and LFB2i dependence, respectively. Interestingly, we found there are 16 common microRNAs which potentially target 13 common mRNAs among the three piPSC lines...
August 31, 2017: Cell Death & Disease
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