keyword
https://read.qxmd.com/read/38473071/whole-genome-scanning-for-selection-signatures-reveals-candidate-genes-associated-with-growth-and-tail-length-in-sheep
#1
JOURNAL ARTICLE
Taotao Li, Meilin Jin, Huihua Wang, Wentao Zhang, Zehu Yuan, Caihong Wei
Compared to Chinese indigenous sheep, Western sheep have rapid growth rate, larger physique, and higher meat yield. These excellent Western sheep were introduced into China for crossbreeding to expedite the enhancement of production performance and mutton quality in local breeds. Here, we investigated population genetic structure and genome-wide selection signatures among the Chinese indigenous sheep and the introduced sheep based on whole-genome resequencing data. The PCA, N-J tree and ADMIXTURE results showed significant genetic difference between Chinese indigenous sheep and introduced sheep...
February 22, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38430033/circular-rna-circ_0096041-promotes-osteosarcoma-cell-proliferation-and-migration-via-sponging-mir-556-5p-and-regulating-lin28a-expression
#2
JOURNAL ARTICLE
Fulin Zhou, Hao Wu, Zhen Yin, Weibo Zhou, Wei Chen
Strategies targeting lin-28 homolog A (LIN28A) for the treatment of osteosarcoma are limited, even though salient findings have illustrated the crucial role of LIN28A in bone deformities and cancer. In the present study, we proved circ_0096041, one of the circular RNAs (circRNAs) with significant upregulated expression in osteosarcoma, to be notably engaged in the progression of osteosarcoma. We elucidated that osteosarcoma patients with highly expressed circ_0096041 had relatively worse prognoses. We determined that circ_0096041 potentially sponge miR-556-5p using the Circular RNA Interactome database...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38341436/dynamic-nucleolar-phase-separation-influenced-by-non-canonical-function-of-lin28a-instructs-pluripotent-stem-cell-fate-decisions
#3
JOURNAL ARTICLE
Tianyu Tan, Bo Gao, Hua Yu, Hongru Pan, Zhen Sun, Anhua Lei, Li Zhang, Hengxing Lu, Hao Wu, George Q Daley, Yu Feng, Jin Zhang
LIN28A is important in somatic reprogramming and pluripotency regulation. Although previous studies addressed that LIN28A can repress let-7 microRNA maturation in the cytoplasm, few focused on its role within the nucleus. Here, we show that the nucleolus-localized LIN28A protein undergoes liquid-liquid phase separation (LLPS) in mouse embryonic stem cells (mESCs) and in vitro. The RNA binding domains (RBD) and intrinsically disordered regions (IDR) of LIN28A contribute to LIN28A and the other nucleolar proteins' phase-separated condensate establishment...
February 10, 2024: Nature Communications
https://read.qxmd.com/read/38321535/identification-of-specific-markers-for-human-pluripotent-stem-cell-derived-small-extracellular-vesicles
#4
JOURNAL ARTICLE
Zhengsheng Chen, Lei Luo, Teng Ye, Jiacheng Zhou, Xin Niu, Ji Yuan, Ting Yuan, Dehao Fu, Haiyan Li, Qing Li, Yang Wang
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinical translational potential in multiple aging-related degenerative diseases. Characterizing the PSC-sEVs is crucial for their clinical applications. However, the specific marker pattern of PSC-sEVs remains unknown. Here, the sEVs derived from two typical types of PSCs including induced pluripotent stem cells (iPSC-sEVs) and embryonic stem cells (ESC-sEVs) were analysed using proteomic analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS), and surface marker phenotyping analysis by nanoparticle flow cytometry (NanoFCM)...
February 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38320485/direct-reprogramming-of-non-limb-fibroblasts-to-cells-with-properties-of-limb-progenitors
#5
JOURNAL ARTICLE
Yuji Atsuta, ChangHee Lee, Alan R Rodrigues, Charlotte Colle, Reiko R Tomizawa, Ernesto G Lujan, Patrick Tschopp, Laura Galan, Meng Zhu, Joshua M Gorham, Jean-Pierre Vannier, Christine E Seidman, Jonathan G Seidman, Marian A Ros, Olivier Pourquié, Clifford J Tabin
The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts...
February 5, 2024: Developmental Cell
https://read.qxmd.com/read/38301803/lin28a-cardiomyocyte-specific-modified-mrna-translation-system-induces-cardiomyocyte-cell-division-and-cardiac-repair
#6
JOURNAL ARTICLE
Ajit Magadum, Jiacheng Sun, Neha Singh, Ann Anu Kurian, Elena Chepurko, Anthony Fargnoli, Roger Hajjar, Jianyi Zhang, Lior Zangi
The mammalian heart has a limited regenerative capacity. Previous work suggested the heart can regenerate during development and immediately after birth by inducing cardiomyocyte (CM) proliferation; however, this capacity is lost seven days after birth. modRNA gene delivery, the same technology used successfully in the two mRNA vaccines against SARS-CoV-2, can prompt cardiac regeneration, cardiovascular regeneration and cardiac protection. We recently established a novel CM-specific modRNA translational system (SMRTs) that allows modRNA translation only in CMs...
January 30, 2024: Journal of Molecular and Cellular Cardiology
https://read.qxmd.com/read/38242343/arf6-a-component-of-intercellular-bridges-is-essential-for-spermatogenesis-in-mice
#7
JOURNAL ARTICLE
Hetty N Wong, Tingfang Chen, P Jeremy Wang, Lawrence B Holzman
Male germ cells are connected by intercellular bridges (ICBs) in a syncytium due to incomplete cytokinesis. Syncytium is thought to be important for synchronized germ cell development by interchange of cytoplasmic factors via ICBs. Mammalian ADP-ribosylation factor 6 (ARF6) is a small GTPase that is involved in many cellular mechanisms including but not limited to regulating cellular structure, motility, vesicle trafficking and cytokinesis. ARF6 localizes to ICBs in spermatogonia and spermatocytes in mice. Here we report that mice with global depletion of ARF6 in adulthood using Ubc-CreERT2 display no observable phenotypes but are male sterile...
January 17, 2024: Developmental Biology
https://read.qxmd.com/read/38235335/growth-suppressor-micrornas-mediate-synaptic-overgrowth-and-behavioral-deficits-in-fragile-x-mental-retardation-protein-deficiency
#8
JOURNAL ARTICLE
Megha Subramanian, William T Mills, Manish D Paranjpe, Uche S Onuchukwu, Manasi Inamdar, Amanda R Maytin, Xinbei Li, Joel L Pomerantz, Mollie K Meffert
Abnormal neuronal and synapse growth is a core pathology resulting from deficiency of the Fragile X mental retardation protein (FMRP), but molecular links underlying the excessive synthesis of key synaptic proteins remain incompletely defined. We find that basal brain levels of the growth suppressor let-7 microRNA (miRNA) family are selectively lowered in FMRP-deficient mice and activity-dependent let-7 downregulation is abrogated. Primary let-7 miRNA transcripts are not altered in FMRP-deficiency and posttranscriptional misregulation occurs downstream of MAPK pathway induction and elevation of Lin28a, a let-7 biogenesis inhibitor...
January 19, 2024: IScience
https://read.qxmd.com/read/38216964/exosomes-derived-from-cancer-associated-fibroblasts-promote-tumorigenesis-metastasis-and-chemoresistance-of-colorectal-cancer-by-upregulating-circ_0067557-to-target-lin28
#9
JOURNAL ARTICLE
Cheng Yang, Yan Zhang, Mingze Yan, Jiahao Wang, Jiaming Wang, Muhong Wang, Yuhong Xuan, Haiyue Cheng, Jiaao Ma, Cuicui Chai, Mingzhe Li, Zhiwei Yu
BACKGROUND: Cancer associated fibroblasts (CAFs) can remodel tumor microenvironment by secreting exosomes. This study aimed to investigate the role of exosomes derived from cancer-associated fibroblasts in colorectal cancer (CRC) progression. METHODS: Circular RNA (circRNA) array was used to identify differentially expressed circRNAs in exosomes from normal fibroblasts (NFs) and CAFs, and confirmed one differentially expressed circRNA circ_0067557 by real-time PCR...
January 12, 2024: BMC Cancer
https://read.qxmd.com/read/38193536/seizure-induced-lin28a-disrupts-pattern-separation-via-aberrant-hippocampal-neurogenesis
#10
JOURNAL ARTICLE
In-Young Choi, Jung-Ho Cha, Seong Yun Kim, Jenny Hsieh, Kyung-Ok Cho
Prolonged seizures can disrupt stem cell behavior in the adult hippocampus, an important brain structure for spatial memory. Here, using a mouse model of pilocarpine-induced status epilepticus (SE), we characterized spatiotemporal expression of Lin28a mRNA and proteins after SE. Unlike Lin28a transcripts, induction of LIN28A protein after SE was detected mainly in the subgranular zone, where immunoreactivity was found in progenitors, neuroblasts, and immature and mature granule neurons. To investigate roles of LIN28A in epilepsy, we generated Nestin-Cre:Lin28aloxP/loxP (conditional KO [cKO]) and Nestin-Cre:Lin28a+/+ (WT) mice to block LIN28A upregulation in all neuronal lineages after acute seizure...
January 9, 2024: JCI Insight
https://read.qxmd.com/read/38167913/role-of-upf1-lin28a-interaction-during-early-differentiation-of-pluripotent-stem-cells
#11
JOURNAL ARTICLE
Seungwon Jung, Seung Hwan Ko, Narae Ahn, Jinsam Lee, Chang-Hwan Park, Jungwook Hwang
UPF1 and LIN28A are RNA-binding proteins involved in post-transcriptional regulation and stem cell differentiation. Most studies on UPF1 and LIN28A have focused on the molecular mechanisms of differentiated cells and stem cell differentiation, respectively. We reveal that LIN28A directly interacts with UPF1 before UPF1-UPF2 complexing, thereby reducing UPF1 phosphorylation and inhibiting nonsense-mediated mRNA decay (NMD). We identify the interacting domains of UPF1 and LIN28A; moreover, we develop a peptide that impairs UPF1-LIN28A interaction and augments NMD efficiency...
January 2, 2024: Nature Communications
https://read.qxmd.com/read/38154448/wnt-signaling-blockade-is-essential-for-maintaining-the-pluripotency-of-chicken-embryonic-stem-cells
#12
JOURNAL ARTICLE
Ryota Kajihara, Ryo Ezaki, Kennosuke Ichikawa, Tenkai Watanabe, Takumi Terada, Mei Matsuzaki, Hiroyuki Horiuchi
Activation of Wnt/β-catenin signaling supports the self-renewal of mouse embryonic stem cells. We aimed to understand the effects of Wnt signaling activation or inhibition on chicken embryonic stem cells (chESCs), as these effects are largely unknown. When the glycogen synthase kinase-3 β inhibitor CHIR99021-which activates Wnt signaling-was added to chESC cultures, the colony shape flattened, and the expression levels of pluripotency-related (NANOG, SOX2, SOX3, OCT4, LIN28A, DNMT3B, and PRDM14) and germ cell (CVH and DAZL) markers showed a decreasing trend, and the growth of chESCs was inhibited after approximately 7 d...
December 7, 2023: Poultry Science
https://read.qxmd.com/read/38135347/identification-of-pan-cancer-testis-genes-and-validation-of-therapeutic-targeting-in-triple-negative-breast-cancer-lin28a-based-and-siglece-based-vaccination-induces-antitumor-immunity-and-inhibits-metastasis
#13
JOURNAL ARTICLE
Jason A Carter, Bharati Matta, Jenna Battaglia, Carter Somerville, Benjamin D Harris, Margaret LaPan, Gurinder S Atwal, Betsy J Barnes
BACKGROUND: Cancer-testis (CT) genes are targets for tumor antigen-specific immunotherapy given that their expression is normally restricted to the immune-privileged testis in healthy individuals with aberrant expression in tumor tissues. While they represent targetable germ tissue antigens and play important functional roles in tumorigenesis, there is currently no standardized approach for identifying clinically relevant CT genes. Optimized algorithms and validated methods for accurate prediction of reliable CT antigens (CTAs) with high immunogenicity are also lacking...
December 22, 2023: Journal for Immunotherapy of Cancer
https://read.qxmd.com/read/38134923/generation-of-canine-induced-pluripotent-stem-cells-under-feeder-free-conditions-using-sendai-virus-vector-encoding-six-canine-reprogramming-factors
#14
JOURNAL ARTICLE
Masaya Tsukamoto, Kazuto Kimura, Takumi Yoshida, Miyuu Tanaka, Mitsuru Kuwamura, Taro Ayabe, Genki Ishihara, Kei Watanabe, Mika Okada, Minoru Iijima, Mahito Nakanishi, Hidenori Akutsu, Kikuya Sugiura, Shingo Hatoya
Although it is in its early stages, canine induced pluripotent stem cells (ciPSCs) hold great potential for innovative translational research in regenerative medicine, developmental biology, drug screening, and disease modeling. However, almost all ciPSCs were generated from fibroblasts, and available canine cell sources for reprogramming are still limited. Furthermore, no report is available to generate ciPSCs under feeder-free conditions because of their low reprogramming efficiency. Here, we reanalyzed canine pluripotency-associated genes and designed canine LIN28A, NANOG, OCT3/4, SOX2, KLF4, and C-MYC encoding Sendai virus vector, called 159cf...
December 19, 2023: Stem Cell Reports
https://read.qxmd.com/read/38028604/computational-study-of-the-motor-neuron-protein-kif5a-to-identify-nssnps-bioactive-compounds-and-its-key-regulators
#15
JOURNAL ARTICLE
Rupesh Kumar, Thirumurthy Madhavan, Kalaiarasan Ponnusamy, Honglae Sohn, Shazia Haider
Introduction: Kinesin family member 5A ( KIF5A ) is a motor neuron protein expressed in neurons and involved in anterograde transportation of organelles, proteins, and RNA. Variations in the KIF5A gene that interfere with axonal transport have emerged as a distinguishing feature in several neurodegenerative disorders, including hereditary spastic paraplegia (HSP10), Charcot-Marie-Tooth disease type 2 (CMT2), and Amyotrophic Lateral Sclerosis (ALS). Methods: In this study, we implemented a computational structural and systems biology approach to uncover the role of KIF5A in ALS...
2023: Frontiers in Genetics
https://read.qxmd.com/read/37984514/exploring-lin28-proteins-unravelling-structure-and-functions-with-emphasis-on-nervous-system-malignancies
#16
REVIEW
Ahmed Maklad, Mohammed Sedeeq, Kai Man Chan, Nuri Gueven, Iman Azimi
Cancer and stem cells share many characteristics related to self-renewal and differentiation. Both cell types express the same critical proteins that govern cellular stemness, which provide cancer cells with the growth and survival benefits of stem cells. LIN28 is an example of one such protein. LIN28 includes two main isoforms, LIN28A and LIN28B, with diverse physiological functions from tissue development to control of pluripotency. In addition to their physiological roles, LIN28A and LIN28B affect the progression of several cancers by regulating multiple cancer hallmarks...
November 18, 2023: Life Sciences
https://read.qxmd.com/read/37927133/methylation-based-subclassifications-of-embryonal-tumor-with-multilayered-rosettes-in-not-just-pediatric-brains
#17
JOURNAL ARTICLE
Eric Eunshik Kim, Kwanghoon Lee, Ji-Hoon Phi, Min-Sung Kim, Hyoung Jin Kang, Hongseok Yun, Sung-Hye Park
The aim of this study is to investigate the genetic profiles and methylation-based classifications of Embryonal tumor with multilayered rosettes (ETMR), with a specific focus on differentiating between C19MC amplified and C19MC-not amplified groups, including cases with DICER1 mutations. To achieve this, next-generation sequencing using a targeted gene panel for brain tumors and methylation class studies using the Epic850K microarray were performed to identify tumor subclasses and their clinicopathological characteristics...
October 31, 2023: Experimental Neurobiology
https://read.qxmd.com/read/37920133/embryonal-tumor-with-multilayered-rosettes-arising-from-the-internal-auditory-canal-of-an-adult-illustrative-case-with-molecular-investigations
#18
Adam Sheriff, Hirokazu Takami, Shunsaku Takayanagi, Yosuke Kitagawa, Shota Tanaka, Masako Ikemura, Reiko Matsuura, Yuko Matsushita, Koichi Ichimura, Nobuhito Saito
Embryonal tumors with multilayered rosettes (ETMRs) are aggressive central nervous system (CNS) tumors that usually occur in young children. Here, we describe the first incidence of ETMR in an adult patient that also originated in the novel location of the internal auditory canal (IAC). The 36-year-old patient initially presented with unsteadiness, diplopia, and tinnitus. The tumor in the IAC was discovered on brain magnetic resonance imaging, and gross total resection was performed followed by pathological and molecular diagnosis...
November 2, 2023: Neuropathology: Official Journal of the Japanese Society of Neuropathology
https://read.qxmd.com/read/37868985/lin28-regulates-thymic-growth-and-involution-and-correlates-with-mhcii-expression-in-thymic-epithelial-cells
#19
JOURNAL ARTICLE
Shiyun Xiao, Wen Zhang, Jie Li, Nancy R Manley
Thymic epithelial cells (TECs) are essential for T cell development in the thymus, yet the mechanisms governing their differentiation are not well understood. Lin28, known for its roles in embryonic development, stem cell pluripotency, and regulating cell proliferation and differentiation, is expressed in endodermal epithelial cells during embryogenesis and persists in adult epithelia, implying postnatal functions. However, the detailed expression and function of Lin28 in TECs remain unknown. In this study, we examined the expression patterns of Lin28 and its target Let-7g in fetal and postnatal TECs and discovered opposing expression patterns during postnatal thymic growth, which correlated with FOXN1 and MHCII expression...
2023: Frontiers in Immunology
https://read.qxmd.com/read/37774938/blocking-the-interaction-between-circtnrc18-and-lin28a-promotes-trophoblast-epithelial-mesenchymal-transformation-and-alleviates-preeclampsia
#20
JOURNAL ARTICLE
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Defects in migration and invasion caused by dysregulation of trophoblastic epithelial-mesenchymal transformation (EMT) play a vital role in preeclampsia (PE). We have previously shown that circTNRC18 inhibits the migration and EMT of trophoblasts; however, its role in PE remains unknown. Herein, we demonstrate that circTNRC18 interacts with an RNA-binding protein, lin-28 homolog A (LIN28A), and this interaction is enhanced in PE placental tissue. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, whereas LIN28A knockdown promotes them...
September 27, 2023: Molecular and Cellular Endocrinology
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