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https://www.readbyqxmd.com/read/28641505/in-utero-exposure-to-di-n-butyl-phthalate-induces-morphological-and-biochemical-changes-in-rats-postpuberty
#1
Yuya Okayama, Shin Wakui, Michael F Wempe, Mitsuru Sugiyama, Masaya Motohashi, Tomoko Mutou, Hiroyuki Takahashi, Eisuke Kume, Hiroshi Ikegami
Pregnant Sprague-Dawley rats were orally administered di( n-butyl)phthalate (DBP; 100 mg/kg/day) on gestation days (GD) 12 to 21. We investigated the male offspring and probed morphological alterations in Sertoli cells at 7, 9, 14, and 17 weeks of age. Parameters assessed in this study included offspring number, sex ratios, body weights, testis weights, seminiferous tubule (ST) profile numbers and diameters, number of vimentin-labeled Sertoli cells, and both testosterone and follicle-stimulating hormone (FSH) levels...
June 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28638318/maturation-behavioral-activation-and-connectivity-of-adult-born-medium-spiny-neurons-in-a-striatal-song-nucleus
#2
Jennifer Kosubek-Langer, Lydia Schulze, Constance Scharff
Neurogenesis continues in the adult songbird brain. Many telencephalic song control regions incorporate new neurons into their existing circuits in adulthood. One song nucleus that receives many new neurons is Area X. Because this striatal region is crucial for song learning and song maintenance the recruitment of new neurons into Area X could influence these processes. As an entry point into addressing this possibility, we investigated the maturation and connectivity within the song circuit and behavioral activation of newly generated Area X neurons...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28632772/characterization-of-slow-cycling-cells-in-the-mouse-cochlear-lateral-wall
#3
Yang Li, Kotaro Watanabe, Masato Fujioka, Kaoru Ogawa
Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days...
2017: PloS One
https://www.readbyqxmd.com/read/28631574/microrna-125a-5p-plays-a-role-as-a-tumor-suppressor-in-lung-carcinoma-cells-by-directly-targeting-stat3
#4
Lou Zhong, Siyuan Sun, Jiahai Shi, Fei Cao, Xiao Han, Zhong Chen
Increasing evidence supports that the dysregulation of microRNA expression plays an important role in the process of tumor occurrence and development. Studies have found that mir-125a-5p expression was downregulated in a variety of tumors, but the effects and mechanism of mir-125a-5p in lung cancer are still unclear. The aim of this study is to detect the expression of mir-125a-5p in lung cancer tissues and lung cancer cell lines and to explore the effects of mir-125a-5p on the biological characteristics of lung cancer cells; thus, this study aims to provide new methods and new strategies for the treatment of lung cancer...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28628625/neural-progenitor-cell-proliferation-in-the-hypothalamus-is-involved-in-acquired-heat-tolerance-in-long-term-heat-acclimated-rats
#5
Kentaro Matsuzaki, Masanori Katakura, Naotoshi Sugimoto, Toshiko Hara, Michio Hashimoto, Osamu Shido
Constant exposure to moderate heat facilitates progenitor cell proliferation and neuronal differentiation in the hypothalamus of heat-acclimated (HA) rats. In this study, we investigated neural phenotype and responsiveness to heat in HA rats' hypothalamic newborn cells. Additionally, the effect of hypothalamic neurogenesis on heat acclimation in rats was evaluated. Male Wistar rats (5 weeks old) were housed at an ambient temperature (Ta) of 32°C for 6 days (STHA) or 40 days (LTHA), while control (CN) rats were kept at a Ta of 24°C for 6 days (STCN) or 40 days (LTCN)...
2017: PloS One
https://www.readbyqxmd.com/read/28627461/8-methoxypsoralen-reduces-akt-phosphorylation-induces-intrinsic-and-extrinsic-apoptotic-pathways-and-suppresses-cell-growth-of-sk-n-as-neuroblastoma-and-sw620-metastatic-colon-cancer-cells
#6
Magdalena Bartnik, Adrianna Sławińska-Brych, Aleksandra Żurek, Martyna Kandefer-Szerszeń, Barbara Zdzisińska
ETHNOPHARMACOLOGICAL RELEVANCE: 8-methoxypsoralen (8-MOP) is a furanocoumarin and an active compound of a traditional Egyptian medicinal plant Ammi majus L, whose juice/fruit has been used for many years in folk phototherapy for the treatment of vitiligo or a hyperproliferative skin disorder, psoriasis. 8-MOP together with UVA light is also used as an anticancer drug for the treatment of cutaneous T-cell lymphoma. However, furanocoumarins exert anticancer activity even without UV irradiation...
June 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28625776/beta-carotene-preferentially-regulates-chicken-myoblast-proliferation-withdrawal-and-differentiation-commitment-via-bco1-activity-and-retinoic-acid-production
#7
C Praud, S Al Ahmadieh, E Voldoire, Y Le Vern, E Godet, N Couroussé, B Graulet, E Le Bihan Duval, C Berri, M J Duclos
The enzyme β-carotene oxygenase 1 (BCO1) catalyzes the breakdown of provitamin A, including beta-carotene (BC), into retinal, prior to its oxidation into retinoic acid (RA). Allelic variation at the BCO1 locus results in differential expression of its mRNA and affects carotenoid metabolism specifically in chicken Pectoralis major muscle. In this context, the aim of this study was to evaluate the potential myogenic effect of BC and the underlying mechanisms in chicken myoblasts. BCO1 mRNA was detected in myoblasts derived from chicken satellite cells...
June 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28620140/comparison-of-tumor-biology-of-two-distinct-cell-sub-populations-in-lung-cancer-stem-cells
#8
Jianyu Wang, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Hongmei Rosie Xing
3Characterization of the stem-like properties of cancer stem cells (CSCs) remain indirect and qualitative, especially the ability of CSCs to undergo asymmetric cell division for self renewal and differentiation, a unique property of cells of stem origin. It is partly due to the lack of stable cellular models of CSCs. In this study, we developed a new approach for CSC isolation and purification to derive a CSC-enriched cell line (LLC-SE). By conducting five consecutive rounds of single cell cloning using the LLC-SE cell line, we obtained two distinct sub-population of cells within the Lewis lung cancer CSCs that employed largely symmetric division for self-renewal (LLC-SD) or underwent asymmetric division for differentiation (LLC-ASD)...
June 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28618656/role-of-growth-factor-receptor-bound-2-in-ccl4-induced-hepatic-fibrosis
#9
Shanfei Ge, Ying Xiong, Xiaoping Wu, Jianping Xie, Fei Liu, Jinni He, Tianxing Xiang, Na Cheng, Lingling Lai, Yuanbin Zhong
BACKGROUND: Growth Factor Receptor-bound 2 (GRB2) plays a crucial role in regulation of cellular function including proliferation and differentiation, and we previously identified GRB2 as promoting HSCs (HSCs) proliferation. However, the underlying mechanisms that are involving in the regulation of GRB2 in hepatic fibrogenesis remain unknown. METHODS: In the present study, we tested the function of GRB2 in hepatic fibrosis. Hepatic fibrosis was induced by subcutaneous CCl4 administration at a dose of 3mL/kg in rats...
June 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28613279/nox-mediated-impairment-of-pdgf-induced-dna-synthesis-in-peripheral-blood-lymphocytes-of-children-with-idiopathic-nephrotic-syndrome
#10
Amal Al-Eisa, Gursev S Dhaunsi
BackgroundCellular oxidative stress, inflammatory responses, and immunogenic events are involved in pathogenesis of idiopathic nephrotic syndrome (INS); however, the exact mechanism remains unknown. We examined NADPH oxidase (NOX) activity and platelet-derived growth factor (PDGF)-induced DNA synthesis in peripheral blood lymphocytes (PBL) of patients with INS.MethodsPBL from 15 patients with INS and 15 age- and gender-matched controls were isolated, and enzyme activities of NOX, catalase, and superoxide dismutase (SOD) were measured along with the assay of malondialdehyde levels and bromo-deoxyuridine incorporation...
June 14, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28612523/-protective-effects-of-intraventricular-transplanted-human-umbilical-cord-derived-mesenchymal-stem-cells-on-hypoxic-ischemic-brain-damages-in-rats
#11
Xue Fan, Xiao-Hong Bai, Juan Chen, Qian Wan, Qiang Chen, De-Zhi Mu
OBJECTIVES: To determine the therapeutic effect and protective mechanism of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on newborn rats with hypoxia ischemic brain damage (HIBD). METHODS: Umbilical cord (3-4 cm) was collected from a healthy male infant for preparation of hUC-MSCs using explants technique. The hUC-MSCs were cultured and labeled with BrdU. The differentiation function of MSCs was identified. Healthy SPF grade neonatal SD rats were randomly divided into sham (n =30), HIBD (n =36) and hUC-MSCs treated HIBD (n =32) groups...
March 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28611594/overexpression-of-mineralocorticoid-receptors-in-the-mouse-forebrain-partly-alleviates-the-effects-of-chronic-early-life-stress-on-spatial-memory-neurogenesis-and-synaptic-function-in-the-dentate-gyrus
#12
Sofia Kanatsou, Henk Karst, Despoina Kortesidou, Rachelle A van den Akker, Jan den Blaauwen, Anjanette P Harris, Jonathan R Seckl, Harm J Krugers, Marian Joels
Evidence from human studies suggests that high expression of brain mineralocorticoid receptors (MR) may promote resilience against negative consequences of stress exposure, including childhood trauma. We examined, in mice, whether brain MR overexpression can alleviate the effects of chronic early life stress (ELS) on contextual memory formation under low and high stress conditions, and neurogenesis and synaptic function of dentate gyrus granular cells. Male mice were exposed to ELS by housing the dam with limited nesting and bedding material from postnatal day (PND) 2 to 9...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28611394/identification-of-the-ectoenzyme-cd38-as-a-marker-of-committed-preadipocytes
#13
A Carrière, Y Jeanson, J-A Côté, C Dromard, A Galinier, S Menzel, C Barreau, S Dupuis-Coronas, E Arnaud, A Girousse, V Cuminetti, J Paupert, B Cousin, C Sengenes, F Koch-Nolte, A Tchernof, L Casteilla
BACKGROUND/OBJECTIVES: Characterization of the adipocyte cellular lineage is required for a better understanding of white adipose tissue homeostasis and expansion. While several studies have focused on the phenotype of the most immature adipocyte progenitors, very few tools exist to identify committed cells. In haematopoiesis, the CD38 ectoenzyme is largely used to delineate various stages of stem cell lineage commitment. We hypothesize that this marker could be used to identify committed preadipocytes...
June 14, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28606778/hmgb1-promotes-neurovascular-remodeling-via-rage-in-the-late-phase-of-subarachnoid-hemorrhage
#14
Xiaodi Tian, Liang Sun, Dongxia Feng, Qing Sun, Yang Dou, Chenglin Liu, Feng Zhou, Haiying Li, Haitao Shen, Zhong Wang, Gang Chen
High-mobility group box1 (HMGB1) is a nuclear protein widely expressed in the central nervous system. Extracellular HMGB1 serves as a proinflammatory cytokine and contributes to brain injury during the acute stage post-stroke. Recently, increasing evidence has demonstrated beneficial effects of HMGB1 in some types of brain injury, but little is known about its effects during the late phase of subarachnoid hemorrhage (SAH). This study was designed to explore the potential roles and mechanisms of HMGB1 and its receptor, receptor for advanced glycation end-products (Rage), on brain recovery in the late stage of experimental SAH...
June 9, 2017: Brain Research
https://www.readbyqxmd.com/read/28605875/mir-1254-promotes-lung-cancer-cell-proliferation-by-targeting-sfrp1
#15
Hong Li, Tian Yang, Dong Shang, Zhongmin Sun
Lung cancer is the leading cause of cancer deaths worldwide, many miRNAs play critical role in lung cancer initiation and progression. Here, we demonstrated that miR-1254 was upregulated in lung cancer tissues and cells. miR-1254 overexpression promoted lung cancer cell proliferation determined by MTT assay, colony formation assay, soft agar growth ability assay and BrdU incorporation assay, miR-1254 knockdown suppressed lung cancer cell proliferation. Mechanism analysis revealed that Wnt/β-catenin pathway antagonist secreted frizzled related protein 1 (SFRP1) was its target, its expression was opposite to SFRP1 level, and directly bound to the 3'UTR of SFRP1...
June 8, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28603047/tadalafil-induces-antiproliferation-apoptosis-and-phosphodiesterase-type-5-downregulation-in-idiopathic-pulmonary-arterial-hypertension-in-vitro
#16
Aya Yamamura, Eri Fujitomi, Naoki Ohara, Kikuo Tsukamoto, Motohiko Sato, Hisao Yamamura
Idiopathic pulmonary arterial hypertension (IPAH) is a fatal disease of the pulmonary artery resulting from a currently unidentified etiology. IPAH is pathologically characterized as sustained vasoconstriction and vascular remodeling of the pulmonary artery. Vascular remodeling is mediated by enhanced proliferation and reduced apoptosis in pulmonary arterial smooth muscle cells (PASMCs). Based on its pathological mechanism, specific phosphodiesterase type 5 (PDE5) inhibitors have been used in the treatment of IPAH...
June 8, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28597506/skeletal-myofiber-vegf-is-required-for-the-exercise-training-induced-increase-in-dentate-gyrus-neuronal-precursor-cells
#17
Benjamin Rich, Miriam Scadeng, M Yamaguchi, Peter D Wagner, Ellen C Breen
Exercise signals neurogenesis in the dentate gyrus of the hippocampus. This phenomenon requires vascular endothelial growth factor (VEGF) originating from outside the blood brain barrier, but no cellular source has been identified. Thus, we hypothesized that VEGF produced by skeletal myofibers plays a role in regulating hippocampal neuronal precursor cell proliferation following exercise training. This was tested in adult conditional skeletal myofiber-specific VEGF gene-ablated mice (VEGF(HSA-/-) ) by providing VEGF(HSA-/-) and non-ablated (VEGF(f/f) ) littermates with running wheels for 14 days...
June 9, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28594400/the-role-of-oligodendrocyte-precursor-cells-expressing-the-gpr17-receptor-in-brain-remodeling-after-stroke
#18
Elisabetta Bonfanti, Paolo Gelosa, Marta Fumagalli, Leda Dimou, Francesca Viganò, Elena Tremoli, Mauro Cimino, Luigi Sironi, Maria P Abbracchio
Following stroke-induced neuronal damage, quiescent oligodendrocyte precursors (OPCs) are activated to proliferate and later to differentiate to myelin-producing cells. GPR17, a receptor transiently expressed on early OPCs, has emerged as a target to implement stroke repair through stimulation of OPC maturation. However, being GPR17 completely downregulated in myelin-producing oligodendrocytes, its actual role in determining the final fate of OPCs after cerebral ischemia is still uncertain. Here, to univocally define the spatiotemporal changes and final fate of GPR17-expressing OPCs, we induced ischemia by middle cerebral artery occlusion (MCAo) in reporter GPR17iCreER(T2):CAG-eGreen florescent protein (GFP) mice, in which, upon tamoxifen treatment, cells expressing GPR17 become green and traceable for their entire life...
June 8, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28592981/acupuncture-stimulation-at-gb34-restores-mptp-induced-neurogenesis-impairment-in-the-subventricular-zone-of-mice
#19
Hyongjun Jeon, Sun Ryu, Dongsoo Kim, Sungtae Koo, Ki-Tae Ha, Seungtae Kim
Adult neurogenesis has recently been considered a new therapeutic paradigm of Parkinson's disease. In this study, we investigated whether acupuncture restores 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- (MPTP-) induced impaired neurogenesis in the subventricular zone (SVZ). Male C57BL/6 mice were given 30 mg/kg of MPTP intraperitoneally once a day for 5 days, after which they were intraperitoneally injected with 50 mg/kg of bromodeoxyuridine (BrdU) and given acupuncture stimulation at HT7 or GB34 for 12 consecutive days...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28590418/enhancement-of-x-ray-induced-apoptosis-by-mobile-phone-like-radio-frequency-electromagnetic-fields-in-mouse-spermatocyte-derived-cells
#20
Ke-Ying Zhang, Hui Xu, Le Du, Jun-Ling Xing, Bin Zhang, Qiang-Shan Bai, Yu-Qiao Xu, Yong-Chun Zhou, Jun-Ping Zhang, Yan Zhou, Gui-Rong Ding
To explore the combined effects of environmental radio-frequency (RF) field and X-ray, mouse spermatocyte-derived (GC-1) cells were exposed to 1950 MHz RF field at specific absorption rate (SAR) of 3 W/kg for 24 h combined with or without X-ray irradiation at 6 Gy. After treatment, the cell proliferation level was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) Assay and 5-Bromo-2-deoxy Uridine (BrdU) enzyme linked immunosorbent (ELISA) Assay. The apoptosis level was detected by annexin V flow cytometry assay, transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) Assay and Caspase-3 Activity Assay...
June 7, 2017: International Journal of Environmental Research and Public Health
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