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https://www.readbyqxmd.com/read/29675577/dna-in-squid-synaptosomes
#1
Carolina Cefaliello, Marina Prisco, Marianna Crispino, Antonio Giuditta
The synthesis of brain metabolic DNA (BMD) is modulated by learning and circadian oscillations and is not involved in cell division or DNA repair. Data from rats have highlighted its prevalent association with the mitochondrial fraction and its lack of identity with mtDNA. These features suggested that BMD could be localized in synaptosomes that are the major contaminants of brain mitochondrial fractions. The hypothesis has been examined by immunochemical analyses of the large synaptosomes of squid optic lobes that are readily prepared and identified...
April 19, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29675370/effect-of-senescence-marker-protein-30-on-the-proliferation-and-apoptosis-of-human-lens-epithelial-cells-sra01-04
#2
Xi Chen, Song-Man Li, Yan-Wei Li, Zi-Hao Han, Hao Liang
AIM: To study the effect of senescence marker protein 30 (SMP30) on the proliferation and apoptosis of human lens epithelial cell (HLEC) SRA01/04. METHODS: SMP30 overexpression (OE) and knock down (KD) type cell lines were cultivated by using two groups regucalcin (RGN; SMP30) lentiviral vectors (LV-RGN, LV-RGN-RNAi) and the respective negative control virus infect SRA01/04 cells. Western blot and real-time quantitative polymerase chain reaction (q-PCR) analysis were used to determine RGN overexpression and knock down efficiency...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29672156/ursodeoxycholic-acid-protects-against-intestinal-barrier-breakdown-by-promoting-enterocyte-migration-via-egfr-and-cox-2-dependent-mechanisms
#3
Jamie M Golden, Oswaldo H Escobar, Michelle Vl Nguyen, Michael U Mallicote, Patil Kavarian, Mark R Frey, Christopher P Gayer
The intestinal barrier is often disrupted in disease states, and intestinal barrier failure leads to sepsis. Ursodeoxycholic acid (UDCA) is a bile acid that may protect the intestinal barrier. We hypothesized that UDCA would protect the intestinal epithelium in injury models. To test this hypothesis, we utilized an in vitro wound healing assay and a mouse model of intestinal barrier injury. We found that UDCA stimulates intestinal epithelial cell migration in vitro, and this migration was blocked by inhibition of COX-2, EGFR, or ERK...
April 19, 2018: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29669322/microrna-381-favors-repair-of-nerve-injury-through-regulation-of-the-sdf-1-cxcr4-signaling-pathway-via-lrrc4-in-acute-cerebral-ischemia-after-cerebral-lymphatic-blockage
#4
Jian-Min Piao, Wei Wu, Zhong-Xi Yang, Yu-Zhen Li, Qi Luo, Jin-Lu Yu
BACKGROUND/AIMS: Acute cerebral ischemia is a manifestation of cerebral vascular insufficiency and has a high mortality. However, the therapy for acute cerebral ischemia is still limited. This study aimed to investigate the effect of microRNA-381 (miR-381) on the repair of nerve injury in rats with acute cerebral ischemia after cerebral lymphatic blockage (CLB) by targeting leucine-rich repeat C4 protein (LRRC4) through the Stromal cell-derived factor-1/CXC chemokine receptor-4 signaling pathway...
April 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29667269/effects-of-bmp-2-on-the-osteogenic-differentiation-of-bone-marrow-stem-cells-in-fibrous-dysplasia
#5
Soyoun Um, Hui Young Kim, Byoung-Moo Seo
OBJECTIVES: The purpose of this study was to evaluate the effects of BMP-2 on bone-grafting procedures for treatment of fibrous dysplasia based on in vitro and in vivo experiments. SUBJECTS AND METHODS: Proliferation of stem cells was determined by colony-forming assay, CCK-8 assay, and BrdU staining. OCT4 and NANOG expression was analyzed by flow cytometry and immunocytochemistry. Osteogenic differentiation was assessed by measuring ALP activity, alizarin red S staining, and in vivo transplantation...
April 18, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29665410/the-role-of-the-rx-homeobox-gene-in-retinal-progenitor-proliferation-and-cell-fate-specification
#6
H M Rodgers, V J Huffman, V A Voronina, M Lewandoski, P H Mathers
The Retinal homeobox gene (Rx; also Rax) plays a crucial role in the early development of the vertebrate eye. Germline deletion of Rx in mice results in the failure of optic vesicle formation, leading to anophthalmia. Recent research using conditional mouse knockout models provides some clues to the role of Rx in eye development following optic vesicle formation. However, the functions of Rx in embryonic retinogenesis are still not fully understood. We investigated the function of Rx in the mouse neural retina using a conditional knockout where the Pax6α-Cre driver deletes Rx activity in early retinal progenitors...
April 14, 2018: Mechanisms of Development
https://www.readbyqxmd.com/read/29663876/phytochemical-screening-antiproliferative-and-antioxidant-properties-of-various-extracts-from-endemic-origanum-acutidens
#7
Feyza Oke-Altuntas, Mehmet Ali Demirci, Ibrahim Demirtas, Ayse Sahin Yaglioglu, Lutfi Behcet
AIM AND OBJECTIVE: Origanum acutidens (Hand.-Mazz.) Ietsw. is an endemic and perennial plant grown mainly in East Anatolia. Recently, natural plant products have attracted interest due to their safety and therapeutic effects. Therefore, the aim of this study was to investigate phytochemical contents and biological effects of Origanum acutidens. MATERIALS AND METHODS: The aerial parts of O. acutidens were extracted with water, ethyl acetate, n-butanol, and methanol/chloroform solvents...
April 16, 2018: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/29663684/adult-neurogenesis-in-the-central-olfactory-pathway-of-dendrobranchiate-and-caridean-shrimps-new-insights-into-the-evolution-of-the-deutocerebral-proliferative-system-in-reptant-decapods
#8
Christin Wittfoth, Steffen Harzsch
Persistent neurogenesis in the central olfactory pathway characterizes many reptant decapods such as lobsters, crayfish and crabs. In these animals, the deutocerebral proliferative system generates new neurons which integrate into the neuronal network of the first order processing neuropil of the olfactory system, the deutocerebral chemosensory lobes (also called olfactory lobes). However, differences concerning the phenotype and the mechanisms that drive adult neurogenesis were reported in crayfish versus spiny lobsters...
April 16, 2018: Developmental Neurobiology
https://www.readbyqxmd.com/read/29663136/memory-retrieval-induced-activation-of-adult-born-neurons-generated-in-response-to-damage-to-the-dentate-gyrus
#9
Andrea Aguilar-Arredondo, Angélica Zepeda
The dentate gyrus (DG) is a neurogenic structure that exhibits functional and structural reorganization after injury. Neurogenesis and functional recovery occur after brain damage, and the possible relation between both processes is a matter of study. We explored whether neurogenesis and the activation of new neurons correlated with DG recovery over time. We induced a DG lesion in young adult rats through the intrahippocampal injection of kainic acid and analyzed functional recovery and the activation of new neurons after animals performed a contextual fear memory task (CFM) or a control spatial exploratory task...
April 16, 2018: Brain Structure & Function
https://www.readbyqxmd.com/read/29651535/cell-cycle-of-the-enamel-knot-during-tooth-morphogenesis
#10
Seo-Yoon Jung, David William Green, Han-Sung Jung, Eun-Jung Kim
Enamel knot (EK) is known to be a central organ in tooth development, especially for cusp patterning. To trace the exact position and movement among the inner dental epithelium (IDE) and EK cells, and to monitor the relationship between the EK and cusp patterning, it is essential that we understand the cell cycle status of the EK in early stages of tooth development. In this study, thymidine analogous (IdU, BrdU) staining was used to evaluate the cell cycle phase of the primary EK at the early casp stage (E13...
April 12, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29651134/endothelial-cell-phenotypes-are-maintained-during-angiogenesis-in-cultured-microvascular-networks
#11
Jessica M Motherwell, Christopher R Anderson, Walter L Murfee
A challenge in tissue engineering biomimetic models for studying angiogenesis is building the physiological complexity of real microvascular networks. Our laboratory recently introduced the rat mesentery culture model as an ex vivo experimental platform for investigating multicellular dynamics involved in angiogenesis within intact microvascular networks. The objective of this study was to compare endothelial cell phenotypes along capillary sprouts in cultured ex vivo rat mesentery microvascular networks to in vivo endothelial cell phenotypes...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649974/delayed-and-repeated-intranasal-delivery-of-bone-marrow-stromal-cells-increases-regeneration-and-functional-recovery-after-ischemic-stroke-in-mice
#12
Monica J Chau, Todd C Deveau, Xiaohuan Gu, Yo Sup Kim, Yun Xu, Shan Ping Yu, Ling Wei
BACKGROUND: Stroke is a leading cause of death and disability worldwide, yet there are limited treatments available. Intranasal administration is a novel non-invasive strategy to deliver cell therapy into the brain. Cells delivered via the intranasal route can migrate from the nasal mucosa to the ischemic infarct and show acute neuroprotection as well as functional benefits. However, there is little information about the regenerative effects of this transplantation method in the delayed phase of stroke...
April 12, 2018: BMC Neuroscience
https://www.readbyqxmd.com/read/29626565/antidepressant-like-actions-by-silencing-of-neuronal-elav-like-rna-binding-proteins-hub-and-huc-in-a-model-of-depression-in-male-mice
#13
Maria Domenica Sanna, Alessandro Quattrone, Nicoletta Galeotti
Currently available antidepressant drugs often fail to achieve full remission and patients might evolve to treatment resistance, showing the need to achieve a better therapy of depressive disorders. Increasing evidence supports that post-transcriptional regulation of gene expression is important in neuronal development and survival and a relevant role is played by RNA binding proteins (RBP). To explore new therapeutic strategies, we investigated the role of the neuron-specific ELAV-like RBP (HuB, HuC, HuD) in a mouse model of depression...
April 4, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29626562/a-human-relevance-investigation-of-ppar%C3%AE-mediated-key-events-in-the-hepatocarcinogenic-mode-of-action-of-propaquizafop-in-rats
#14
Christian Strupp, Werner H Bomann, François Spézia, Frédéric Gervais, Roy Forster, Lysiane Richert, Pramila Singh
Propaquizafop is an herbicide with demonstrated hepatocarcinogenic activity in rodents. A rodent-specific mode of action (MOA) in the liver via activation of peroxisome proliferator-activated receptor α (PPARα) has been postulated based on existing data. Experience with PPARα-inducing pharmaceuticals indicates a lack of human relevance of this MOA. The objective of the present investigation was to evaluate the dependency of early key events leading to liver tumors on PPARα activation in wildtype (WT) compared to PPARα-knockout (KO) rats following 2 weeks exposure to 75, 500 and 1000 ppm propaquizafop in the diet...
April 4, 2018: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29625437/metalloproteinase-adamts16-is-required-for-proper-closure-of-the-optic-fissure
#15
Mingzhe Cao, Jiamin Ouyang, Jingyi Guo, Siyuan Lin, Shuyi Chen
Purpose: Coloboma is a sight-threatening congenital eye disease caused by a failure in optic fissure (OF) closure. The aim of this study was to investigate the role of Adamts16, a metalloproteinase, in OF closure. Methods: RNA in situ hybridization was used to examine the expression of Adamts16 in developing mouse and zebrafish eyes. Morpholino knockdowns were performed to study adamts16 function during zebrafish eye development. Additionally, immunofluorescent staining, RNA in situ hybridization, bromodeoxyuridine (BrdU) labeling, TUNEL assays, and high-throughput sequencing were used to examine altered cellular and molecular events in adamts16-morphant optic cups (OCs)...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29625115/administration-of-placenta-derived-mesenchymal-stem-cells-counteracts-a-delayed-anergic-state-following-a-transient-induction-of-endogenous-neurogenesis-activity-after-global-cerebral-ischemia
#16
A Ra Kho, Ok Joon Kim, Jeong Hyun Jeong, Ji Min Yu, Hye Sun Kim, Bo Young Choi, Sang Won Suh, Tae Nyoung Chung
BACKGROUND: Global cerebral ischemia (GCI) is a major obstacle for cardiac arrest survival. Recent studies have suggested the possibility of mesenchymal stem cell (MSC) as a novel therapeutic option for GCI, but these results were limited to the neuroprotective effects of MSCs. Therefore, we aimed to investigate specific characteristics of neurogenesis after transient GCI, and to assess the effect of MSC on these characteristics. METHODS: Adult male Sprague-Dawley rats were subjected to 7 minutes of transient GCI and randomized into 7 groups: baseline, MSC, and control administered groups, to be analyzed at 2, 3, and 4 weeks after GCI, respectively...
April 3, 2018: Brain Research
https://www.readbyqxmd.com/read/29623936/cell-proliferation-during-hair-cell-regeneration-induced-by-math-1-in-vestibular-epithelia-in-vitro
#17
Yi-Bo Huang, Rui Ma, Juan-Mei Yang, Zhao Han, Ning Cong, Zhen Gao, Dongdong Ren, Jing Wang, Fang-Lu Chi
Hair cell regeneration is the fundamental method of correcting hearing loss and balance disorders caused by hair cell damage or loss. How to promote hair cell regeneration is a hot focus in current research. In mammals, cochlear hair cells cannot be regenerated and few vestibular hair cells can be renewed through spontaneous regeneration. However, Math1 gene transfer allows a few inner ear cells to be transformed into hair cells in vitro or in vivo. Hair cells can be renewed through two possible means in birds: supporting cell differentiation and transdifferentiation with or without cell division...
March 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29621758/targeting-mtor-by-cz415-inhibits-head-and-neck-squamous-cell-carcinoma-cells
#18
Jing Xie, Quan Li, Xi Ding, Yunyun Gao
BACKGROUND/AIMS: mTOR is an important therapeutic target for human head and neck squamous cell carcinoma (HNSCC). The current study tested the anti-HNSCC cell activity by a mTOR kinase inhibitor CZ415. METHODS: HNSCC cells were treated with CZ415. Cell death was tested by lactate dehydrogenase (LDH) assay and MTT assay. Cell proliferation was tested by BrdU ELISA assay and [H3] thymidine incorporation assay, with apoptosis assayed by the TUNEL staining. A Western blotting assay was applied to test autophagy-associated proteins, mTOR and signalings...
March 29, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29618746/mirna-142-3p-increases-radiosensitivity-in-human-umbilical-cord-blood-mononuclear-cells-by-inhibiting-the-expression-of-cd133
#19
Fang Yuan, Lu Liu, Yonghong Lei, Yi Hu
This study is to explore the molecular regulation mechanism of CD133 which is associated with malignancy and poor prognosis of blood system diseases. CD133+HUCB-MNC (human umbilical cord blood mononuclear cells) and CD133-HUCB-MNC were isolated and amplificated from umbilical cord blood, and then were exposed to different doses of radiation and subjected to a clonogenic assay. CCK-8 kit was used to detect cell viability, Annexin V-FITC/PI cell apoptosis detection kit was used for the detection of apoptotic cells and the BrdU assay was performed by flow cytometry...
April 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29617677/the-mtor-kinase-inhibitor-cz415-inhibits-human-papillary-thyroid-carcinoma-cell-growth
#20
Xiaobin Li, Zongze Li, Yimin Song, Wenjing Liu, Ziwen Liu
BACKGROUND/AIM: Mammalian target of rapamycin (mTOR) plays an important role in papillary thyroid carcinoma (PTC) cell progression. CZ415 is a novel, highly-efficient and specific mTOR kinase inhibitor. The current study tested the potential anti-tumor activity of CZ415 in human PTC cells. METHODS: The established (TPC-1 cell line) and primary human PTC cells were treated with CZ415. Cell survival and growth were tested by Cell Counting Kit-8 assay and BrdU ELISA assay, respectively...
March 28, 2018: Cellular Physiology and Biochemistry
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