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CD24 CD38

Geothy Chakupurakal, María Alejandra Garcia-Marquez, Alexander Shimabukuro-Vornhagen, Sandra Kluth, Hans Schlosser, Sebastian Theurich, Christof Scheid, Michael Hallek, Udo Holtick, Michael von Bergwelt-Baildon
OBJECTIVE: The role of B-cells and the subgroup of IL-10 producing B-cells, known to have a regulatory function, in patients following a haematopoetic stem cell transplant (alloSCT) has not been clearly understood to date. METHODS: We prospectively recruited 95 patients following an alloSCT and studied the B-cell reconstitution on days 30, 90 and 150. Regulatory B10-cells could be analysed in 22 consecutively recruited patients on day 30 post transplant. RESULTS: The total B-cell percentages in transplant recipients (median 0...
September 26, 2016: European Journal of Haematology
M Kabuto, N Fujimoto, T Takahashi, T Tanaka
BACKGROUND: hile the frequency of IL-10-producing B cells (B10 cells) is reported to have an inverse correlation with disease activity in some human autoimmune diseases, the association between B10 cells and autoimmune blistering diseases (AIBD) has not been well-evaluated. Although several phenotypes of human regulatory B cells have been proposed, the most appropriate one was not established. OBJECTIVE: This study aimed to evaluate B10 cells in AIBD including their phenotypes...
October 7, 2016: British Journal of Dermatology
Sindhu Cherian, Valerie Miller, Vivian McCullouch, Katy Dougherty, Jonathan R Fromm, Brent L Wood
BACKGROUND: Residual disease detection following therapy is an important prognostic variable in B-lymphoblastic leukemia (B-LL). Most flow cytometric strategies for detecting B cell malignancy utilize CD19 to identify B cells. With growing use of anti-CD19 targeted therapies, alternative strategies are needed for residual disease detection. We describe an approach for residual disease detection in this setting. METHODS: A novel combination was designed using expression of CD22 or CD24 (without CD66b) for B cell detection in combination with markers aberrantly expressed in B-LL (CD10, CD20, CD34, CD38, and CD45)...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Willem van de Veen, Barbara Stanic, Oliver F Wirz, Kirstin Jansen, Anna Globinska, Mübeccel Akdis
Immune tolerance to both self-antigens and innocuous non-self-antigens is essential to protect the host against chronic inflammatory diseases and tissue damage. A wide range of cell types and suppressive molecules are involved in induction and maintenance of tolerance. In addition to their key function in the production of immunoglobulins, B cells can regulate immune responses through their surface molecules and secretion of cytokines. Regulatory B (Breg) cells are characterized by their immunosuppressive capacity, which is often mediated through IL-10 secretion...
September 2016: Journal of Allergy and Clinical Immunology
Jian Rong Sheng, Kourosh Rezania, Betty Soliven
Regulatory B cells (Bregs) attenuate the severity of experimental autoimmune myasthenia gravis (EAMG) in an interleukin-10 (IL-10)-dependent manner. The goal of this study was to investigate the role of human Bregs in MG focusing on CD19(+)CD1d(hi) CD5(+) and CD19(+)CD24(hi)CD38(hi) subsets. We found that MG patients exhibited a decrease in the frequency of both Breg subsets and IL-10 producing B cells within each subset, which correlated with disease severity. In addition, there was impaired suppression of Th1 polarization in MG...
August 15, 2016: Journal of Neuroimmunology
Laurent Simonin, Elisabeth Pasquier, Christophe Leroyer, Divi Cornec, Julie Lemerle, Boutahar Bendaoud, Sophie Hillion, Jacques-Olivier Pers, Francis Couturaud, Yves Renaudineau
Among patients with venous thromboembolism (VTE), the persistent detection of antiphospholipid (aPL) antibodies (Ab) represents an independent high risk factor for recurrence. However, oral anticoagulation vitamin K antagonist therapy, frequently used in these patients, is problematic in assessing and/or confirming a diagnosis of primary aPL syndrome (pAPS), suggesting use of alternative strategies. For this reason, and by analogy with other autoimmune diseases, a flow cytometer approach testing peripheral T cell subsets (CD3, CD4, and CD8), B cell subsets (B1, transitional, naive, and memory), and NK cells can be proposed...
June 25, 2016: Clinical Reviews in Allergy & Immunology
J Bigot, C Pilon, M Matignon, C Grondin, C Leibler, A Aissat, F Pirenne, J L Cohen, P Grimbert
The role of B cells after transplant regarding allograft rejection or tolerance has become a topic of major interest. Recently, in renal transplant recipients, a B cell signature characterized by the overexpression of CD19(+) CD38(hi) CD24(hi) transitional B cells has been observed in operationally tolerant patients and in belatacept-treated patients with significantly lower incidence of donor-specific antibodies. The phenotypic and functional characterization of these transitional B cells is far from exhaustive...
June 7, 2016: American Journal of Transplantation
Wenli Li, Xiaolan Tian, Xin Lu, Qinglin Peng, Xiaoming Shu, Hanbo Yang, Yuanli Li, Yan Wang, Xuezhi Zhang, Qingyan Liu, Guochun Wang
Regulatory B cells (Bregs) are critical in maintaining self-tolerance. Their role in dermatomyositis (DM), an autoimmune disease characterized by inappropriate regulation of hyperactivated B and T cells, has not been clearly defined. In the current study, we performed flow cytometry analysis of studied CD19(+) CD24(high)CD38(high) Breg subpopulations in blood samples from 30 patients with DM, 37 diseased controls and 23 healthy controls. A significant decrease was observed in the frequency of Bregs in DM patients compared to that in diseased controls (p < 0...
2016: Scientific Reports
Jorge Lima, Catarina Martins, Maria J Leandro, Glória Nunes, Maria-José Sousa, Jorge C Branco, Luís-Miguel Borrego
BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women...
2016: BMC Pregnancy and Childbirth
Jiyong Song, Li Xiao, Guosheng Du, Yu Gao, Wen Chen, Shaozhen Yang, Wenmei Fan, Bingyi Shi
Sirolimus can significantly amplify regulatory T cells (Tregs) in vivo and in vitro, but the specific mechanism of this has not been well documented. The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus was investigated in peripheral blood mononuclear cells (PBMCs) in vitro in this study. The results showed that the percentages of both CD19+CD24+CD38+TGF-β1+ Bregs and CD19+CD24+CD38+IL-10+ Bregs to B cells were elevated by Sirolimus in PBMCs including B cells. Sirolimus significantly enhances the cytokine production of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in PBMCs with B cells, and the enhancement significantly decreased in PBMCs without B cells...
September 2016: International Immunopharmacology
Ji-Yong Song, Guo-Sheng Du, Li Xiao, Wen Chen, Long-Long Suo, Yu Gao, Li-Kui Feng, Bing-Yi Shi
BACKGROUND: Lymphocyte subsets play important roles in rejection in liver transplant recipients, and the effect of splenic function on these roles remains unknown. The aim of this study was to explore the feasibility to adjust immunosuppressive agents based on splenic function status through detecting the lymphocyte subsets in liver transplantBeijing recipients. METHODS: The lymphocyte subsets of 49 liver transplant recipients were assessed in the 309th Hospital of Chinese People's Liberation Army between June 2014 and August 2015...
June 5, 2016: Chinese Medical Journal
Honggeng Guan, Yang Lan, Yuqiu Wan, Qin Wang, Cheng Wang, Longjiang Xu, Yongjing Chen, Wenting Liu, Xueguang Zhang, Yecheng Li, Yongping Gu, Zemin Wang, Fang Xie
Accumulating evidence suggests that B cells play important roles in inhibiting the immune response in autoimmune disorders and human tumors as well as murine tumor models. In an effort to explore the role of B cells in human breast cancer etiology, we examined the presence of CD19(+) B lymphocytes in 134 cases of invasive breast carcinoma (IBCa) and 31 breast fibroadenoma, and assessed its relationship with PD-L1 (programmed death-ligand 1) expression in breast cancer. We found that the density of CD19(+) B lymphocytes was higher in IBCa compared with fibroadenoma, and significantly associated with increasing tumor grade, negative estrogen status...
February 2016: Oncoimmunology
Bastian Tebbe, Benjamin Wilde, Zeng Ye, Junyu Wang, Xinning Wang, Fu Jian, Sebastian Dolff, Manfred Schedlowski, Peter F Hoyer, Andreas Kribben, Oliver Witzke, André Hoerning
BACKGROUND: CD19+CD24hiCD38hi transitional immature B-lymphocytes have been demonstrated to play an important role in regulating the alloimmune response in transplant recipients. Here, we analyzed the effect of calcineurin inhibition on these peripherally circulating regulatory B-cells (Breg) in renal transplant recipients receiving cyclosporine A (CsA) or tacrolimus. METHODS: PBMCs from healthy subjects (HS) (n = 16) and renal transplant recipients (n = 46) were isolated...
2016: PloS One
Steven K Lundy, Qi Wu, Qin Wang, Catherine A Dowling, Sophina H Taitano, Guangmei Mao, Yang Mao-Draayer
OBJECTIVE: To test the hypothesis that dimethyl fumarate (Tecfidera, BG-12) affects B-cell subsets in patients with relapsing-remitting multiple sclerosis (RRMS). METHODS: Peripheral blood B cells were compared for surface marker expression in patients with RRMS prior to initiation of treatment, after 4-6 months, and at more than 1 year of treatment with BG-12. Production of interleukin (IL)-10 by RRMS patient B cells was also analyzed. RESULTS: Total numbers of peripheral blood B lymphocytes declined after 4-6 months of BG-12 treatment, due to losses in both the CD27+ memory B cells and CD27(neg) B-cell subsets...
April 2016: Neurology® Neuroimmunology & Neuroinflammation
Xinrui Wang, Yonggang Zhu, Manli Zhang, Hongjuan Wang, Yanfang Jiang, Pujun Gao
BACKGROUND AND AIMS: Ulcerative colitis (UC) is characterized by Th2-polarized, B cell responses. However, neither size, composition, nor activity of the Breg compartment in active UC has been evaluated. METHODS: Peripheral blood of 25 patients with UC, 5 patients with Crohn's disease (CD) and 15 control subjects was examined for Bregs subsets by flow cytometry. Intestinal tissue of 5 patients with UC, 3 patients with CD and 5 control subjects was also examined for Bregs...
October 2016: Journal of Crohn's & Colitis
Madhvi Menon, Paul A Blair, David A Isenberg, Claudia Mauri
Signals controlling the generation of regulatory B (Breg) cells remain ill-defined. Here we report an "auto"-regulatory feedback mechanism between plasmacytoid dendritic cells (pDCs) and Breg cells. In healthy individuals, pDCs drive the differentiation of CD19(+)CD24(hi)CD38(hi) (immature) B cells into IL-10-producing CD24(+)CD38(hi) Breg cells and plasmablasts, via the release of IFN-α and CD40 engagement. CD24(+)CD38(hi) Breg cells conversely restrained IFN-α production by pDCs via IL-10 release. In systemic lupus erythematosus (SLE), this cross-talk was compromised; pDCs promoted plasmablast differentiation but failed to induce Breg cells...
March 15, 2016: Immunity
K Heinemann, B Wilde, A Hoerning, B Tebbe, A Kribben, O Witzke, S Dolff
OBJECTIVES: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by B cell-dependent autoantibody production. Recently, a new B-cell subset was discovered that has a regulatory capacity. The aim of this study was to analyse regulatory B cells (Bregs) in SLE patients. METHOD: Peripheral mononuclear blood cells (PBMCs) of 34 SLE patients fulfilling the American College of Rheumatology (ACR) criteria for SLE and 21 healthy controls (HC) were included...
July 2016: Scandinavian Journal of Rheumatology
Gábor Papp, Edit Gyimesi, Krisztina Szabó, Éva Zöld, Margit Zeher
Recently, we reported elevated proportions of circulating follicular T helper cells and higher levels of interleukin- (IL-) 21 in primary Sjögren's syndrome (pSS). Interaction of invariant natural killer T (iNKT) cells with B cells and granzyme B (GrB) production may be also important in pSS. Thirty-two pSS patients and 24 healthy controls were enrolled in our study. We investigated the expression of intracellular GrB and IL-21 receptor (IL-21R) of CD19(+)CD5(+) and CD19(+)CD5(-) B cells; furthermore, we determined the IL-21 expression of iNKT cells as well...
2016: Mediators of Inflammation
Asmaa M Zahran, Khalid I Elsayh, Khaled Saad, Mostafa Embaby, Ahmed M Ali
BACKGROUND: β-Thalassemia major (BTM) is considered the most common hemoglobinopathy in Egypt and is one of the major health problems in our locality. MATERIALS & METHODS: We investigated the frequency of B-regulatory cells (CD19(+)CD38(hi)CD24(hi)); (Bregs) among polytransfused alloimmunized and non-alloimmunized children with BTM. The study included 110 polytransfused pediatric patients with β-thalassemia major. Clinical and transfusion records of all studied patients were reviewed...
March 2016: Blood Cells, Molecules & Diseases
Veronika Svachova, Alena Sekerkova, Petra Hruba, Irena Tycova, Marketa Rodova, Eva Cecrdlova, Janka Slatinska, Eva Honsova, Ilja Striz, Ondrej Viklicky
B cells play an important role in the immune responses which affect the outcomes of kidney allografts. Dynamic changes of B-cell compartments in clinical kidney transplantation are still poorly understood. B-cell subsets were prospectively monitored using flow cytometry for 1 year in 98 kidney transplant recipients. Data were correlated with immunosuppression and clinical outcomes. An increase in the total population of B lymphocytes was observed during the first week after transplantation. The level of IgM(high) CD38(high) CD24(high) transitional B cells reduced significantly up until the third month, with partial repopulation in the first year...
May 2016: Transplant International: Official Journal of the European Society for Organ Transplantation
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