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Kouichi Morita

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https://www.readbyqxmd.com/read/29728909/association-between-dengue-severity-and-plasma-levels-of-dengue-specific-ige-and-chymase
#1
Miho Inokuchi, Shyam Prakash Dumre, Shusaku Mizukami, Mya Myat Ngwe Tun, Mohamed Gomaa Kamel, Dao Huy Manh, Le Hong Phuc, Nguyen Van Thuong, Tran Van An, Lan Nguyen Weiss, Tarek Turk, Tran Ngoc Dang, Vu Thi Que Huong, Kouichi Morita, Nguyen Tien Huy, Kenji Hirayama
There is no definitive predictor of dengue severity, and this has led to a very large number of unnecessary hospitalizations worldwide. Although mast cell mediators are believed to a play role in dengue severity, the lack of precise kinetic data demands further research on early predictors. We enrolled 111 patients with confirmed dengue and 85 with "other febrile illness" (OFI) in a hospital-based prospective study in Vietnam. Dengue patients were classified as level 1, 2, or 3 based on the clinical intervention received...
May 4, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29723109/impact-of-a-six-year-project-to-enhance-the-awareness-of-community-based-palliative-care-on-the-place-of-death
#2
Nozomu Murakami, Kouichi Tanabe, Tatsuya Morita, Yasunaga Fujikawa, Shiro Koseki, Shinya Kajiura, Kazunori Nakajima, Ryuji Hayashi
OBJECT: To examine the clinical outcomes of a project to enhance the awareness of community-based palliative care (awareness-enhancing project), focusing on home death and care rates in communities. METHODS: A single-center study on community-based intervention was conducted. The awareness-enhancing project, consisting of three intervention approaches (outreach, palliative care education for community-based medical professionals, and information-sharing tool use), was executed, and changes in the home death rate in the community were examined...
May 3, 2018: Journal of Palliative Medicine
https://www.readbyqxmd.com/read/29669948/survey-of-tick-borne-zoonotic-viruses-in-wild-deer-in-hokkaido-japan
#3
Leo Uchida, Daisuke Hayasaka, Mya Myat Ngwe Tun, Kouichi Morita, Yasukazu Muramatsu, Katsuro Hagiwara
Tick-borne encephalitis (TBE) and severe fever with thrombocytopenia syndrome (SFTS) are both tick-borne zoonotic diseases caused by TBE virus (TBEV) and SFTS phlebovirus (SFTSV). In 2016, a second domestic TBE case was reported in Hokkaido, Japan, after an absence of 23 years. We conducted IgG ELISA for TBEV and SFTSV on 314 deer (Cervus nippon yesoensis) serum samples collected from 3 places in Hokkaido. There were 7 seropositive samples for TBEV but none for SFTSV by ELISA. The specificity of the 7 positive samples was confirmed by neutralization tests against TBEV, and 5 sera showed 320 to 640 of 50% focus reduction endpoint titers...
April 19, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29429035/inhibitory-effect-of-the-green-tea-molecule-egcg-against-dengue-virus-infection
#4
Muhareva Raekiansyah, Corazon C Buerano, Mark Anthony D Luz, Kouichi Morita
Dengue virus (DENV) infection is a major public health problem worldwide; however, specific antiviral drugs against it are not available. Hence, identifying effective antiviral agents for the prevention of DENV infection is important. In this study, we showed that the reportedly highly biologically active green-tea component epigallocatechin gallate (EGCG) inhibited dengue virus infection regardless of infecting serotype, but no or minimal inhibition was observed with other flaviviruses, including Japanese encephalitis virus, yellow fever virus, and Zika virus...
February 10, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29409678/isolation-and-genomic-characterization-of-culex-flaviviruses-from-mosquitoes-in-myanmar
#5
Aung Kyaw Kyaw, Mya Myat Ngwe Tun, Corazon C Buerano, Takeshi Nabeshima, Miako Sakaguchi, Tsuyoshi Ando, Shingo Inoue, Yi Yi Mya, Daisuke Hayasaka, Hlaing Myat Thu, Kyaw Zin Thant, Kouichi Morita
An entomological surveillance of arboviruses was conducted in Myanmar in 2014. A total of 8357 Culex mosquito vectors were collected in the Mandalay area and virus isolation was done by using the mosquito cell line C6/36 E2. A total of eighteen strains of Culex flavivirus (CxFV) were isolated from Cx. tritaeniorhynchus, Cx. vishnui and Cx. fuscocephala. Like other insect-specific flaviviruses, CxFV can replicate only in mosquito cells but not in mammalian cells. These CxFV strains that were isolated in Japan from mosquitoes collected in Myanmar were closely related to the Wang Thong virus detected from Cx fusocephalus in Thailand and Cx...
March 2, 2018: Virus Research
https://www.readbyqxmd.com/read/29401664/a-simple-mechanism-based-on-amino-acid-substitutions-is-not-a-critical-determinant-of-high-mortality-of-japanese-encephalitis-virus-infection-in-mice
#6
Yuki Takamatsu, Leo Uchida, Muhareva Raekiansyah, Mark Anthony Luz, Kouichi Morita, Daisuke Hayasaka
For the development of effective treatment strategies for Japanese encephalitis (JE), it is important to identify the viral factors causing severe disease during JE virus (JEV) infection. In this study, we assessed whether amino acid substitutions are critical factors for higher mortality of JaTH160 compared with JaOArS982 in mice using the technique of infectious cDNA clones. We raised the possibility that two amino acids of C124 and NS3482 of JaTH160 may contribute to increased mortality in mice. However, simultaneous substitutions of these amino acids did not significantly increase the virulence of JaOArS982, suggesting that high mortality due to JaTH160 viral infection cannot be simply attributed to the specific amino acids...
February 3, 2018: Viruses
https://www.readbyqxmd.com/read/29398122/frequency-and-characteristics-of-the-tbk1-gene-variants-in-japanese-patients-with-sporadic-amyotrophic-lateral-sclerosis
#7
Genki Tohnai, Ryoichi Nakamura, Jun Sone, Masahiro Nakatochi, Daichi Yokoi, Masahisa Katsuno, Hazuki Watanabe, Hirohisa Watanabe, Mizuki Ito, Yuanzhe Li, Yuishin Izumi, Mitsuya Morita, Akira Taniguchi, Osamu Kano, Masaya Oda, Satoshi Kuwabara, Koji Abe, Ikuko Aiba, Koichi Okamoto, Kouichi Mizoguchi, Kazuko Hasegawa, Masashi Aoki, Nobutaka Hattori, Osamu Onodera, Hiroya Naruse, Jun Mitsui, Yuji Takahashi, Jun Goto, Hiroyuki Ishiura, Shinichi Morishita, Jun Yoshimura, Koichiro Doi, Shoji Tsuji, Kenji Nakashima, Ryuji Kaji, Naoki Atsuta, Gen Sobue
Amyotrophic lateral sclerosis (ALS) is a devastating neurological disease, and the etiology of sporadic ALS is generally unknown. The TANK-binding kinase 1 (TBK1) gene was identified as an ALS gene contributing to a predisposition toward ALS. To reveal the frequency and characteristics of variants of the TBK1 gene in sporadic ALS patients in Japan, we analyzed the TBK1 gene by exome sequencing in a large Japanese cohort of 713 sporadic ALS patients and 800 controls. We identified known or potentially toxic rare variants of TBK1 gene in 9 patients (1...
April 2018: Neurobiology of Aging
https://www.readbyqxmd.com/read/29363460/detection-of-zika-virus-infection-in-myanmar
#8
Mya Myat Ngwe Tun, Aung Kyaw Kyaw, Saw Wut Hmone, Shingo Inoue, Corazon C Buerano, Aung Min Soe, Meng Ling Moi, Daisuke Hayasaka, Hlaing Myat Thu, Futoshi Hasebe, Kyaw Zin Thant, Kouichi Morita
Zika virus (ZIKV), an emerging mosquito-borne flavivirus, causes a dengue-like infection that has recently caught global attention. The infection, which also includes some birth defects, has been documented in the Americas, Pacific Islands, and some parts of Africa and Asia. There are no published reports on local ZIKV transmission in Myanmar. In this study, a total of 462 serum samples from patients and asymptomatic persons were collected in Myanmar from 2004 to 2017. They were analyzed for ZIKV infection by immunoglobulin M capture enzyme-linked immunosorbent assay (ELISA), immunoglobulin G indirect ELISA, neutralization test, real-time polymerase chain reaction (PCR), and conventional PCR...
March 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29343225/health-worker-knowledge-of-integrated-disease-surveillance-and-response-standard-case-definitions-a-cross-sectional-survey-at-rural-health-facilities-in-kenya
#9
Mitsuru Toda, Dejan Zurovac, Ian Njeru, David Kareko, Matilu Mwau, Kouichi Morita
BACKGROUND: The correct knowledge of standard case definition is necessary for frontline health workers to diagnose suspected diseases across Africa. However, surveillance evaluations commonly assume this prerequisite. This study assessed the knowledge of case definitions for health workers and their supervisors for disease surveillance activities in rural Kenya. METHODS: A cross-sectional survey including 131 health workers and their 11 supervisors was undertaken in two counties in Kenya...
January 17, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29321001/dengue-virus-infection-enhancement-activity-in-neutralizing-antibodies-of-healthy-adults-before-dengue-season-as-determined-by-using-fc%C3%AE-r-expressing-cells
#10
Minh Huong Phu Ly, Meng Ling Moi, Thi Bich Hau Vu, Mya Myat Ngwe Tun, Todd Saunders, Cam Nhat Nguyen, Anh Kieu Thi Nguyen, Hung Manh Nguyen, Than Huu Dao, Do Quyen Pham, Thi Thu Thuy Nguyen, Thi Quynh Mai Le, Futoshi Hasebe, Kouichi Morita
BACKGROUND: Antibodies are critical responses to protect the host from dengue virus(DENV) infection. Antibodies target DENV by two pathologic mechanisms: virus neutralization and infection enhancement. In dengue patients, the absence of neutralizing activity in the presence of FcγR implies that infection-enhancing activity hampers the neutralizing activity of antibodies, which could potentially lead to symptomatic presentations and severe clinical outcomes. METHODS: A total of 100 pair serum samples from adult healthy volunteers were obtained during the dengue season in Ha Noi in 2015 for evaluation of neutralizing and infection-enhancing activity...
January 10, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29315809/analysis-of-vibrio-seventh-pandemic-island-ii-and-novel-genomic-islands-in-relation-to-attachment-sequences-among-a-wide-variety-of-vibrio-cholerae-strains
#11
Tuan Hai Nguyen, Tho Duc Pham, Naomi Higa, Hanako Iwashita, Taichiro Takemura, Makoto Ohnishi, Kouichi Morita, Tetsu Yamashiro
Vibrio cholerae O1 El Tor, the pathogen responsible for the current cholera pandemic, became pathogenic by acquiring virulent factors including Vibrio seventh pandemic islands (VSP)-I and -II. Diversity of VSP-II is well recognized; however, studies addressing attachment sequence left (attL) sequences of VSP-II are few. In this report, a wide variety of V. cholerae strains were analyzed for the structure and distribution of VSP-II in relation to their attachment sequences. Of 188 V. cholerae strains analyzed, 81% (153/188) strains carried VSP-II; of these, typical VSP-II, and a short variant was found in 36% (55/153), and 63% (96/153), respectively...
March 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29165625/evidence-of-chikungunya-virus-circulation-in-the-terai-region-of-nepal-in-2014-and-2015
#12
Kishor Pandey, Basu Dev Pandey, Ram Rekha Chaurasiya, Mahesh Thakur, Biswas Neupane, Yogendra Shah, Mya Myat Ngwe Tun, Kouichi Morita
Background: Chikungunya virus (CHIKV) infection is an emerging viral disease in Nepal, with the reported by our laboratories in 2013. Because of the similarity in signs and symptoms to dengue virus (DENV) and misdiagnosis, the under-reporting of CHIKV infection in DENV-endemic areas of Nepal is very common. The aim of the present study is to identify CHIKV infection among patients with febrile illness during the 2014-2015 outbreaks. Methods: A total of 219 serum samples were tested for CHIKV and DENV by using enzyme-linked immunosorbent assays (ELISAs) to detect immunoglobulin M (IgM) and IgG antibodies, with confirmation by a 50% focal reduction neutralization test (FRNT50)...
July 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29093640/identification-of-novel-antiviral-of-fungus-derived-brefeldin-a-against-dengue-viruses
#13
Muhareva Raekiansyah, Mihoko Mori, Kenichi Nonaka, Masanobu Agoh, Kazuro Shiomi, Atsuko Matsumoto, Kouichi Morita
Microbial natural products possess a wide range of biological and biochemical potential. Among them, fungal secondary metabolites are one of the most important sources for discovering new drugs or lead compounds. In the present study, we explored substances produced by the strain Penicillium sp. FKI-7127 for its antiviral activity. We identified brefeldin A as a novel antiviral agent against dengue viruses. The inhibitory effect of brefeldin A was confirmed by virus titer and immunofluorescence assay. Brefeldin A inhibited dengue viruses regardless of serotypes and other related viruses including Zika virus and Japanese encephalitis virus...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29051976/late-gadolinium-enhancement-on-cardiac-magnetic-resonance-imaging-is-associated-with-coronary-endothelial-dysfunction-in-patients-with-dilated-cardiomyopathy
#14
Mina Nakayama, Megumi Yamamuro, Seiji Takashio, Tomoaki Uemura, Naoki Nakayama, Kyoko Hirakawa, Seitaro Oda, Daisuke Utsunomiya, Koichi Kaikita, Seiji Hokimoto, Yasuyuki Yamashita, Yukiko Morita, Kazuo Kimura, Kouichi Tamura, Kenichi Tsujita
Myocardial fibrosis and coronary endothelial dysfunction are important determinants of outcome in patients with heart failure. However, the relationship of these factors in patients with dilated cardiomyopathy (DCM) is not fully understood. This study aimed to investigate the relationship between endothelium-dependent coronary vasomotor abnormality and late gadolinium enhancement (LGE) on cardiac magnetic resonance imaging (CMR) in patients with DCM. We examined 38 consecutive patients with DCM. All patients underwent CMR and the acetylcholine (ACh) provocation test using cardiac catheterization...
October 19, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28956764/an-envelope-modified-tetravalent-dengue-virus-like-particle-vaccine-has-implications-for-flavivirus-vaccine-design
#15
Akane Urakami, Mya Myat Ngwe Tun, Meng Ling Moi, Atsuko Sakurai, Momoko Ishikawa, Sachiko Kuno, Ryuji Ueno, Kouichi Morita, Wataru Akahata
Dengue viruses (DENV) infect 50 to 100 million people each year. The spread of DENV-associated infections is one of the most serious public health problems worldwide, as there is no widely available vaccine or specific therapeutic for DENV infections. To address this, we developed a novel tetravalent dengue vaccine by utilizing virus-like particles (VLPs). We created recombinant DENV1 to -4 (DENV1-4) VLPs by coexpressing precursor membrane (prM) and envelope (E) proteins, with an F108A mutation in the fusion loop structure of E to increase the production of VLPs in mammalian cells...
December 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28628629/understanding-msos-a-qualitative-study-examining-the-implementation-of-a-text-messaging-outbreak-alert-system-in-rural-kenya
#16
Mitsuru Toda, Ian Njeru, Dejan Zurovac, David Kareko, Shikanga O-Tipo, Matilu Mwau, Kouichi Morita
Outbreaks of epidemic diseases pose serious public health risks. To overcome the hurdles of sub-optimal disease surveillance reporting from the health facilities to relevant authorities, the Ministry of Health in Kenya piloted mSOS (mobile SMS-based disease outbreak alert system) in 2013-2014. In this paper, we report the results of the qualitative study, which examined factors that influence the performances of mSOS implementation. In-depth interviews were conducted with 11 disease surveillance coordinators and 32 in-charges of rural health facilities that took part in the mSOS intervention...
2017: PloS One
https://www.readbyqxmd.com/read/28446808/possible-combinatorial-effects-of-current-smoking-and-alcohol-intake-on-chronic-kidney-disease-in-a-japanese-nationwide-cross-sectional-survey
#17
Kouichi Tamura, Toru Dejima, Yukiko Morita, Satoshi Hirade, Hiromichi Wakui
No abstract text is available yet for this article.
August 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28392469/susceptibility-and-initial-immune-response-of-tupaia-belangeri-cells-to-dengue-virus-infection
#18
Mohammad Enamul Hoque Kayesh, Bouchra Kitab, Takahiro Sanada, Daisuke Hayasaka, Kouichi Morita, Michinori Kohara, Kyoko Tsukiyama-Kohara
Dengue is an emerging disease of great public health significance worldwide. The lack of a suitable infection model has hampered dengue virus (DENV) pathogenesis study, and developing a suitable small animal model has been a long-standing challenge. The aim of this study was to develop a feasible experimental model of DENV infection using Tupaia belangeri. The susceptibility of tupaia to DENV infection and characteristics of its innate immune response were examined in vitro. We found that tupaia fibroblast cells support replication of DENV serotypes 1-4 with a linear increase in viral load 24-96h post-infection in both cells and culture supernatants...
July 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28250260/burden-of-rotavirus-and-enteric-bacterial-pathogens-among-children-under-five-years-old-hospitalized-with-diarrhea-in-suburban-and-rural-areas-in-kenya
#19
Mohammad Shah, Erick Odoyo, Ernest Wandera, Cyrus Kathiiko, Martin Bundi, Gabriel Miringu, Sora Guyo, Satoshi Komoto, James Nyangao, Mohamed Karama, Takao Tsuji, Koki Taniguchi, Kouichi Morita, Yoshio Ichinose
This study aimed to investigate the incidence of rotavirus and enteric bacterial infections among children up to five years old with diarrhea living in suburban and rural areas in Kenya. It was a cross-sectional descriptive study, and a total of 1060 diarrheal fecal specimens were obtained from 722 children at the Kiambu County Hospital (KCH) located in a suburban area and 338 children from the Mbita District Hospital (MDH) located in a rural part of western Kenya, between August 2011 and December 2013. Of the 1060 isolates, group A rotavirus (RVA) was detected in 29...
February 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28108177/discriminating-between-adaptive-and-carcinogenic-liver-hypertrophy-in-rat-studies-using-logistic-ridge-regression-analysis-of-toxicogenomic-data-the-mode-of-action-and-predictive-models
#20
Shujie Liu, Taisuke Kawamoto, Osamu Morita, Kouichi Yoshinari, Hiroshi Honda
Chemical exposure often results in liver hypertrophy in animal tests, characterized by increased liver weight, hepatocellular hypertrophy, and/or cell proliferation. While most of these changes are considered adaptive responses, there is concern that they may be associated with carcinogenesis. In this study, we have employed a toxicogenomic approach using a logistic ridge regression model to identify genes responsible for liver hypertrophy and hypertrophic hepatocarcinogenesis and to develop a predictive model for assessing hypertrophy-inducing compounds...
March 1, 2017: Toxicology and Applied Pharmacology
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