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Kouichi Morita

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https://www.readbyqxmd.com/read/29321001/dengue-virus-infection-enhancement-activity-in-neutralizing-antibodies-of-healthy-adults-before-dengue-season-as-determined-by-using-fc%C3%AE-r-expressing-cells
#1
Minh Huong Phu Ly, Meng Ling Moi, Thi Bich Hau Vu, Mya Myat Ngwe Tun, Todd Saunders, Cam Nhat Nguyen, Anh Kieu Thi Nguyen, Hung Manh Nguyen, Than Huu Dao, Do Quyen Pham, Thi Thu Thuy Nguyen, Thi Quynh Mai Le, Futoshi Hasebe, Kouichi Morita
BACKGROUND: Antibodies are critical responses to protect the host from dengue virus(DENV) infection. Antibodies target DENV by two pathologic mechanisms: virus neutralization and infection enhancement. In dengue patients, the absence of neutralizing activity in the presence of FcγR implies that infection-enhancing activity hampers the neutralizing activity of antibodies, which could potentially lead to symptomatic presentations and severe clinical outcomes. METHODS: A total of 100 pair serum samples from adult healthy volunteers were obtained during the dengue season in Ha Noi in 2015 for evaluation of neutralizing and infection-enhancing activity...
January 10, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29315809/analysis-of-vibrio-seventh-pandemic-island-ii-and-novel-genomic-islands-in-relation-to-attachment-sequences-among-a-wide-variety-of-vibrio-cholerae-strains
#2
Tuan Hai Nguyen, Tho Duc Pham, Naomi Higa, Hanako Iwashita, Taichiro Takemura, Makoto Ohnishi, Kouichi Morita, Tetsu Yamashiro
Vibrio cholerae O1 El Tor, the pathogen responsible for the current cholera pandemic became pathogenic by acquiring virulent factors including Vibrio seventh pandemic islands (VSP)-I and -II. Diversity of VSP-II are well recognized, however, studies addressing attachment sequence left (attL) sequences of VSP-II are few. In this report, a wide variety of V. cholerae strains were analyzed for the structure and distribution of VSP-II in relation to their attachment sequences. Of 188 V. cholerae strains analyzed, 81% (153/188) strains carried VSP-II, of those, typical VSP-II, and a short variant was found in 36% (55/153), and 63% (96/153), respectively...
January 8, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29165625/evidence-of-chikungunya-virus-circulation-in-the-terai-region-of-nepal-in-2014-and-2015
#3
Kishor Pandey, Basu Dev Pandey, Ram Rekha Chaurasiya, Mahesh Thakur, Biswas Neupane, Yogendra Shah, Mya Myat Ngwe Tun, Kouichi Morita
Background: Chikungunya virus (CHIKV) infection is an emerging viral disease in Nepal, with the reported by our laboratories in 2013. Because of the similarity in signs and symptoms to dengue virus (DENV) and misdiagnosis, the under-reporting of CHIKV infection in DENV-endemic areas of Nepal is very common. The aim of the present study is to identify CHIKV infection among patients with febrile illness during the 2014-2015 outbreaks. Methods: A total of 219 serum samples were tested for CHIKV and DENV by using enzyme-linked immunosorbent assays (ELISAs) to detect immunoglobulin M (IgM) and IgG antibodies, with confirmation by a 50% focal reduction neutralization test (FRNT50)...
November 20, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29093640/identification-of-novel-antiviral-of-fungus-derived-brefeldin-a-against-dengue-viruses
#4
Muhareva Raekiansyah, Mihoko Mori, Kenichi Nonaka, Masanobu Agoh, Kazuro Shiomi, Atsuko Matsumoto, Kouichi Morita
Microbial natural products possess a wide range of biological and biochemical potential. Among them, fungal secondary metabolites are one of the most important sources for discovering new drugs or lead compounds. In the present study, we explored substances produced by the strain Penicillium sp. FKI-7127 for its antiviral activity. We identified brefeldin A as a novel antiviral agent against dengue viruses. The inhibitory effect of brefeldin A was confirmed by virus titer and immunofluorescence assay. Brefeldin A inhibited dengue viruses regardless of serotypes and other related viruses including Zika virus and Japanese encephalitis virus...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29051976/late-gadolinium-enhancement-on-cardiac-magnetic-resonance-imaging-is-associated-with-coronary-endothelial-dysfunction-in-patients-with-dilated-cardiomyopathy
#5
Mina Nakayama, Megumi Yamamuro, Seiji Takashio, Tomoaki Uemura, Naoki Nakayama, Kyoko Hirakawa, Seitaro Oda, Daisuke Utsunomiya, Koichi Kaikita, Seiji Hokimoto, Yasuyuki Yamashita, Yukiko Morita, Kazuo Kimura, Kouichi Tamura, Kenichi Tsujita
Myocardial fibrosis and coronary endothelial dysfunction are important determinants of outcome in patients with heart failure. However, the relationship of these factors in patients with dilated cardiomyopathy (DCM) is not fully understood. This study aimed to investigate the relationship between endothelium-dependent coronary vasomotor abnormality and late gadolinium enhancement (LGE) on cardiac magnetic resonance imaging (CMR) in patients with DCM. We examined 38 consecutive patients with DCM. All patients underwent CMR and the acetylcholine (ACh) provocation test using cardiac catheterization...
October 19, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28956764/an-envelope-modified-tetravalent-dengue-virus-like-particle-vaccine-has-implications-for-flavivirus-vaccine-design
#6
Akane Urakami, Mya Myat Ngwe Tun, Meng Ling Moi, Atsuko Sakurai, Momoko Ishikawa, Sachiko Kuno, Ryuji Ueno, Kouichi Morita, Wataru Akahata
Dengue viruses (DENV) infect 50 to 100 million people each year. The spread of DENV-associated infections is one of the most serious public health problems worldwide, as there is no widely available vaccine or specific therapeutic for DENV infections. To address this, we developed a novel tetravalent dengue vaccine by utilizing virus-like particles (VLPs). We created recombinant DENV1 to -4 (DENV1-4) VLPs by coexpressing precursor membrane (prM) and envelope (E) proteins, with an F108A mutation in the fusion loop structure of E to increase the production of VLPs in mammalian cells...
December 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28628629/understanding-msos-a-qualitative-study-examining-the-implementation-of-a-text-messaging-outbreak-alert-system-in-rural-kenya
#7
Mitsuru Toda, Ian Njeru, Dejan Zurovac, David Kareko, Shikanga O-Tipo, Matilu Mwau, Kouichi Morita
Outbreaks of epidemic diseases pose serious public health risks. To overcome the hurdles of sub-optimal disease surveillance reporting from the health facilities to relevant authorities, the Ministry of Health in Kenya piloted mSOS (mobile SMS-based disease outbreak alert system) in 2013-2014. In this paper, we report the results of the qualitative study, which examined factors that influence the performances of mSOS implementation. In-depth interviews were conducted with 11 disease surveillance coordinators and 32 in-charges of rural health facilities that took part in the mSOS intervention...
2017: PloS One
https://www.readbyqxmd.com/read/28446808/possible-combinatorial-effects-of-current-smoking-and-alcohol-intake-on-chronic-kidney-disease-in-a-japanese-nationwide-cross-sectional-survey
#8
Kouichi Tamura, Toru Dejima, Yukiko Morita, Satoshi Hirade, Hiromichi Wakui
No abstract text is available yet for this article.
August 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28392469/susceptibility-and-initial-immune-response-of-tupaia-belangeri-cells-to-dengue-virus-infection
#9
Mohammad Enamul Hoque Kayesh, Bouchra Kitab, Takahiro Sanada, Daisuke Hayasaka, Kouichi Morita, Michinori Kohara, Kyoko Tsukiyama-Kohara
Dengue is an emerging disease of great public health significance worldwide. The lack of a suitable infection model has hampered dengue virus (DENV) pathogenesis study, and developing a suitable small animal model has been a long-standing challenge. The aim of this study was to develop a feasible experimental model of DENV infection using Tupaia belangeri. The susceptibility of tupaia to DENV infection and characteristics of its innate immune response were examined in vitro. We found that tupaia fibroblast cells support replication of DENV serotypes 1-4 with a linear increase in viral load 24-96h post-infection in both cells and culture supernatants...
April 6, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28250260/burden-of-rotavirus-and-enteric-bacterial-pathogens-among-children-under-five-years-old-hospitalized-with-diarrhea-in-suburban-and-rural-areas-in-kenya
#10
Mohammad Shah, Erick Odoyo, Ernest Wandera, Cyrus Kathiiko, Martin Bundi, Gabriel Miringu, Sora Guyo, Satoshi Komoto, James Nyangao, Mohamed Karama, Takao Tsuji, Koki Taniguchi, Kouichi Morita, Yoshio Ichinose
This study aimed to investigate the incidence of rotavirus and enteric bacterial infections among children up to five years old with diarrhea living in suburban and rural areas in Kenya. It was a cross-sectional descriptive study, and a total of 1060 diarrheal fecal specimens were obtained from 722 children at the Kiambu County Hospital (KCH) located in a suburban area and 338 children from the Mbita District Hospital (MDH) located in a rural part of western Kenya, between August 2011 and December 2013. Of the 1060 isolates, group A rotavirus (RVA) was detected in 29...
February 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28108177/discriminating-between-adaptive-and-carcinogenic-liver-hypertrophy-in-rat-studies-using-logistic-ridge-regression-analysis-of-toxicogenomic-data-the-mode-of-action-and-predictive-models
#11
Shujie Liu, Taisuke Kawamoto, Osamu Morita, Kouichi Yoshinari, Hiroshi Honda
Chemical exposure often results in liver hypertrophy in animal tests, characterized by increased liver weight, hepatocellular hypertrophy, and/or cell proliferation. While most of these changes are considered adaptive responses, there is concern that they may be associated with carcinogenesis. In this study, we have employed a toxicogenomic approach using a logistic ridge regression model to identify genes responsible for liver hypertrophy and hypertrophic hepatocarcinogenesis and to develop a predictive model for assessing hypertrophy-inducing compounds...
March 1, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27825473/corrigendum-to-phagocytic-cells-contribute-to-the-antibody-mediated-elimination-of-pulmonary-infected-sars-coronavirus-virology-2014-157-168
#12
Fumihiko Yasui, Michinori Kohara, Masahiro Kitabatake, Tetsu Nishiwaki, Hideki Fujii, Chise Tateno, Misako Yoneda, Kouichi Morita, Kouji Matsushima, Shigeo Koyasu, Chieko Kai
No abstract text is available yet for this article.
December 2016: Virology
https://www.readbyqxmd.com/read/27795473/dengue-associated-acute-encephalitis-syndrome-cases-in-son-la-province-vietnam-in-2014
#13
Mya Myat Ngwe Tun, Rohitha V Muthugala, Nguyen Thi Thu Thuy, Pham Hoai Linh Ly, Le Thi Hien Thu, Dang Thi Dinh, Nguyen Viet Hoang, Le Thi Quynh Mai, Meng Ling Moi, Corazon C Buerano, Kouichi Morita, Futoshi Hasebe
Acute encephalitis syndrome (AES) is associated with high morbidity and mortality, and affects both children and adults. The main etiologic agent is Japanese encephalitis virus (JEV); however, there are also reports of Dengue virus (DENV) encephalitis. The objectives of this study were to determine the proportion of patients with encephalitis due to JEV during the 2014 outbreak in Son La Province in Vietnam and to explore the association of DENV in non-JEV viral encephalitis cases. Of 90 patients, 6 (6.7%) were positive for anti-JEV immunoglobulin M (IgM), 5 (5...
July 24, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27777508/pathogenic-potential-and-growth-kinetics-of-muko-virus-in-mice-and-human-derived-cells
#14
Gianne Eduard L Ulanday, Satoshi Shimada, Ngwe Tun Mya Myat, Takeshi Nabeshima, Kouichi Morita, Daisuke Hayasaka
BACKGROUND: Ticks have been long known as vectors of various pathogens, some of which can cause high fatality rates among infected individuals. Our enhanced tick surveillance around Nagasaki, Japan, led to the isolation and identification of a new strain of a recently identified Orbivirus, Muko virus (MUV). The orbiviruses have a wide host range, including humans, and is related to a spectrum of clinical outcomes. However, the zoonotic potential of some members of the genus, although reported, were not clearly elucidated...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27554675/hypoglycemia-during-the-temporary-interruption-of-parenteral-nutrition-infusion-in-pediatric-hematopoietic-stem-cell-transplantation
#15
Ryu Yanagisawa, Kouichi Takeuchi, Kazutoshi Komori, Ikuko Fujihara, Yoshihiko Hidaka, Daisuke Morita, Akiko Futatsugi, Takako Ono, Eiko Hidaka, Kazuo Sakashita, Masaaki Shiohara
BACKGROUND: Parenteral nutrition (PN) is required with pediatric procedures such as hematopoietic stem cell transplantation (HSCT). However, risks associated with temporary PN infusion interruption remain unclear. MATERIALS AND METHODS: We retrospectively analyzed in 22 children undergoing HSCT receiving PN with the same daily routine: temporary PN infusion interruption before breakfast for administering a saline-diluted acyclovir drip. After correcting patients' glucose levels, we examined minimum blood glucose levels between preparative regimen initiation and post-HSCT day 30...
August 23, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27551237/development-and-utility-of-an-in-vitro-fluorescence-based-assay-for-the-discovery-of-novel-compounds-against-dengue-2-viral-protease
#16
Gianne Eduard L Ulanday, Kenta Okamoto, Kouichi Morita
BACKGROUND: Dengue disease is one of the most significant vector-borne illnesses in the world. The emergence and re-emergence of dengue infections in many parts of the world affect millions annually and continue to burden public health systems especially in low-income populations. Advances in dengue vaccine development showed promising results; however, protection seems to be suboptimal. There is no licensed chemotherapeutic agent against dengue to date. An ideal scenario of combinatorial vaccination of high-risk individuals and chemotherapy of the diseased during outbreaks may compensate for the meager protection offered by the vaccine...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27493841/persistence-of-neutralizing-antibody-against-dengue-virus-2-after-70-years-from-infection-in-nagasaki
#17
Mya Myat Ngwe Tun, Yoshihito Muta, Shingo Inoue, Kouichi Morita
This study aimed to investigate the duration of humoral immune responses to dengue virus (DENV) infection in Japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in Nagasaki, Japan, from 1942 to 1944. A Japanese volunteer requested serological diagnosis of DENV infection in 2014 and donated blood sample to measure the antibody titer against DENV by antiflavi IgG indirect ELISA, focus reduction neutralization test, and plaque reduction neutralization test...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27433127/molecular-and-serological-epidemiology-of-japanese-encephalitis-virus-jev-in-a-remote-island-of-western-japan-an-implication-of-jev-migration-over-the-east-china-sea
#18
Akira Yoshikawa, Takeshi Nabeshima, Shingo Inoue, Masanobu Agoh, Kouichi Morita
BACKGROUND: Japanese encephalitis (JE) is a mosquito-borne infectious disease caused by Japanese encephalitis virus (JEV). About 1-10 cases with severe central nervous system symptoms have been constantly reported every year in Japan. To clarify the mechanism of maintenance of JEV, the present study surveyed pigs for serological evidence of JEV infection and isolated JEV strains from pigs and mosquitoes in Isahaya City (Isahaya) and Goto City (Goto) in the islets of Goto in Nagasaki Prefecture from 2008 to 2014...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27433125/seroepidemiological-evidence-of-severe-fever-with-thrombocytopenia-syndrome-virus-infections-in-wild-boars-in-nagasaki-japan
#19
Daisuke Hayasaka, Yu Fuxun, Akira Yoshikawa, Guillermo Posadas-Herrera, Satoshi Shimada, Mya Myat Ngwe Tun, Masanobu Agoh, Kouichi Morita
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease in East Asia. It is thought that the SFTS virus (SFTSV) circulates between ticks and animals in nature and that the virus is transmitted to humans by tick bites. SFTS is endemic to Nagasaki in western Japan; however, epidemiological information regarding SFTSV in Nagasaki is not known. In this study, we performed SFTSV IgG ELISAs and neutralization antibody assays for a seroepidemiological survey using samples from wild boars captured in six areas of Nagasaki...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27307452/development-and-characterization-of-monoclonal-antibodies-to-yellow-fever-virus-and-application-in-antigen-detection-and-igm-capture-enzyme-linked-immunosorbent-assay
#20
Ferdinard Adungo, Fuxun Yu, David Kamau, Shingo Inoue, Daisuke Hayasaka, Guillermo Posadas-Herrera, Rosemary Sang, Matilu Mwau, Kouichi Morita
Yellow fever (YF) is an acute hemorrhagic viral infection transmitted by mosquitoes in Africa and South America. The major challenge in YF disease detection and confirmation of outbreaks in Africa is the limited availability of reference laboratories and the persistent lack of access to diagnostic tests. We used wild-type YF virus sequences to generate recombinant envelope protein in an Escherichia coli expression system. Both the recombinant protein and sucrose gradient-purified YF vaccine virus 17D (YF-17D) were used to immunize BALB/c mice to generate monoclonal antibodies (MAbs)...
August 2016: Clinical and Vaccine Immunology: CVI
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