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citrus pectin

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https://www.readbyqxmd.com/read/27842844/rheological-and-chemical-properties-of-pectin-enriched-fractions-from-different-sources-extracted-with-citric-acid
#1
Joanna Mierczyńska, Justyna Cybulska, Artur Zdunek
Yield, properties and functionality of pectins depend on the source material and method of extraction. The objective of this work was to compare pectinolytic enzyme activity in fresh pulp as well as the physicochemical and rheological properties of polysaccharides extracted with citric acid from six new potential sources: fruit materials - peach, blackcurrant, raspberry, strawberry, plum and a vegetable Source: carrot. The uronic acid content of polysaccharides extracted in citric acid depended on pectinolytic enzymes activity in fresh plant tissues and ranged between 16...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27829066/galectin-3-blockade-reduces-renal-fibrosis-in-two-normotensive-experimental-models-of-renal-damage
#2
Ernesto Martinez-Martinez, Jaime Ibarrola, Laurent Calvier, Amaya Fernandez-Celis, Celine Leroy, Victoria Cachofeiro, Patrick Rossignol, Natalia Lopez-Andres
BACKGROUND: Galectin-3 (Gal-3), a β-galactoside-binding lectin, is increased in kidney injury and its pharmacological blockade reduces renal damage in acute kidney injury, hyperaldosteronism or hypertensive nephropathy. We herein investigated the effects of pharmacological Gal-3 inhibition by modified citrus pectin (MCP) in early renal damage associated with obesity and aortic stenosis (AS). RESULTS: Gal-3 was upregulated in kidneys from high fat diet (HFD) rats and in animals with partial occlusion of ascending aorta (AS)...
2016: PloS One
https://www.readbyqxmd.com/read/27815266/role-for-galectin-3-in-calcific-aortic-valve-stenosis
#3
J Rafael Sádaba, Ernesto Martínez-Martínez, Vanessa Arrieta, Virginia Álvarez, Amaya Fernández-Celis, Jaime Ibarrola, Amaia Melero, Patrick Rossignol, Victoria Cachofeiro, Natalia López-Andrés
BACKGROUND: Aortic stenosis (AS) is a chronic inflammatory disease, and calcification plays an important role in the progression of the disease. Galectin-3 (Gal-3) is a proinflammatory molecule involved in vascular osteogenesis in atherosclerosis. Therefore, we hypothesized that Gal-3 could mediate valve calcification in AS. METHODS AND RESULTS: Blood samples and aortic valves (AVs) from 77 patients undergoing AV replacement were analyzed. As controls, noncalcified human AVs were obtained at autopsy (n=11)...
November 4, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27702494/effect-of-low-and-high-methoxyl-citrus-pectin-on-the-properties-of-polypyrrole-based-electroactive-hydrogels
#4
Laís C Lopes, Fernanda F Simas-Tosin, Thales R Cipriani, Luís F Marchesi, Marcio Vidotti, Izabel C Riegel-Vidotti
Electroactive hydrogels were prepared using commercial citric pectin, either raw (PC) or purified through dialysis (dPC), and chemically synthesized polypyrrole (PPy). (1)H NMR analyses showed that PC is a low methoxyl pectin (degree of methoxylation, DM=46%) and dPC is a high methoxyl pectin (DM=77%). The pyrrole polymerization was monitored through UV-vis spectroscopy and both samples were observed to be good stabilizers for PPy in aqueous medium. The dispersions were used to prepare the hydrogels h-PC-PPy and h-dPC-PPy...
January 2, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27696792/immobilization-of-recombinant-pectate-lyase-from-clostridium-thermocellum-atcc-27405-on-magnetic-nanoparticles-for-bioscouring-of-cotton-fabric
#5
Soumyadeep Chakraborty, T Jagan Mohan Rao, Arun Goyal
Recombinant pectate lyase from family 1 polysaccharide lyase (PL1B) was immobilized on synthesized magnetic nanoparticles (MNPs) after 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) activation. At 70 mg/ml MNPs 100% binding of 1 mg/ml PL1B was achieved. The immobilized PL1B-MNP displayed activity of 20.3 and 18.2 U/mg against polygalacturonic acid and citrus pectin, respectively, which was higher than the activity of free PL1B, on the same substrates of 17.8 and 16.2 U/mg. The immobilized PL1B-MNP showed 32 fold and 14 fold enhanced thermal stability at 80°C and 90°C, respectively as compared with free PL1B at same temperatures...
October 3, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27680678/in-vitro-%C3%AE-carotene-bioaccessibility-and-lipid-digestion-in-emulsions-influence-of-pectin-type-and-degree-of-methyl-esterification
#6
Tina A J Verrijssen, Stefanie Christiaens, Sarah H E Verkempinck, Jeroen Boeve, Tara Grauwet, Ann M Van Loey, Laura Salvia-Trujillo, Marc E Hendrickx
Citrus pectin (CP) and sugar beet pectin (SBP) were demethoxylated and fully characterized in terms of pectin properties in order to investigate the influence of the pectin degree of methyl-esterification (DM) and the pectin type on the in vitro β-carotene bioaccessibility and lipid digestion in emulsions. For the CP based emulsions containing β-carotene enriched oil, water and pectin, the β-carotene bioaccessibility, and lipid digestion were higher in the emulsions with pectin with a higher DM (57%; "CP57 emulsion") compared to the emulsions with pectin with a lower DM (30%; "CP30 emulsion") showing that the DM plays an important role...
October 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27644093/yield-esterification-degree-and-molecular-weight-evaluation-of-pectins-isolated-from-orange-and-grapefruit-peels-under-different-conditions
#7
Mohamed Yassine Sayah, Rachida Chabir, Hamid Benyahia, Youssef Rodi Kandri, Fouad Ouazzani Chahdi, Hanan Touzani, Faouzi Errachidi
Orange (Citrus sinensis) and grapefruit (Citrus paradise) peels were used as a source of pectin, which was extracted under different conditions. The peels are used under two states: fresh and residual (after essential oil extraction). Organic acid (citric acid) and mineral acid (sulfuric acid) were used in the pectin extraction. The aim of this study is the evaluation the effect of extraction conditions on pectin yield, degree of esterification "DE" and on molecular weight "Mw". Results showed that the pectin yield was higher using the residual peels...
2016: PloS One
https://www.readbyqxmd.com/read/27622937/preparation-and-self-assembly-mechanism-of-bovine-serum-albumin-citrus-peel-pectin-conjugated-hydrogel-a-potential-delivery-system-for-vitamin-c
#8
Hailong Peng, Sha Chen, Mei Luo, Fangjian Ning, Xuemei Zhu, Hua Xiong
In this study, a novel hydrogel (BSA-pectin hydrogel, BPH) was prepared via a self-assembly method by using the natural polymers of bovine serum albumin (BSA) and citrus peel pectin (pectin). The rheological properties and gel conformational structures were determined and showed that electrostatic and covalent interactions between BSA and pectin were the main mechanisms for the formation of BPH. The morphological characteristics of BPH included a stable and solid three-dimensional network structure with a narrow size distribution (polydispersity index <0...
October 5, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27570277/pattern-of-phenolic-content-antioxidant-activity-and-senescence-related-enzymes-in-granulated-vs-non-granulated-juice-sacs-of-kinnow-mandarin-citrus-nobilis-x-c-deliciosa
#9
R R Sharma, O P Awasthi, Kuldeep Kumar
'Kinnow' is a hybrid mandarin, developed at California (USA) but could not become successful there. However, it revolutionized citrus industry in India, Pakistan and Bangladesh. Recent reports indicate that like other citrus fruits, it also suffers from juice-sac granulation but exact cause of this malady is not known. Fully-mature 'Kinnow' fruits were harvested and observations on some physical and biochemical attributes were recorded and their relationship was established with occurrence of granulation. About 12...
March 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27538689/the-introduction-of-the-fungal-d-galacturonate-pathway-enables-the-consumption-of-d-galacturonic-acid-by-saccharomyces-cerevisiae
#10
Alessandra Biz, Maura Harumi Sugai-Guérios, Joosu Kuivanen, Hannu Maaheimo, Nadia Krieger, David Alexander Mitchell, Peter Richard
BACKGROUND: Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize D-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp...
2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27422378/morphology-of-complexes-formed-between-%C3%AE-lactoglobulin-nanofibrils-and-pectins-is-influenced-by-the-ph-and-structural-characteristics-of-the-pectins
#11
Charith A Hettiarachchi, Laurence D Melton, Martin A K Williams, Duncan J McGillivray, Juliet A Gerrard, Simon M Loveday
For the optimal use of β-lactoglobulin nanofibrils as a raw material in biological composites an in-depth knowledge of their interactions with other constituents is necessary. To understand the effect of electrostatic interactions on the morphology of resulting complexes, β-lactoglobulin nanofibrils were allowed to interact with pectins in which the amount of available negative charge was controlled by selecting their degree of methylesterification. In this study, citrus pectins having different degrees of methylesterification (∼48, 67, 86, and 97%) were selected and interacted with nanofibrils at pH 2 and pH 3, where they possess a net positive charge...
November 2016: Biopolymers
https://www.readbyqxmd.com/read/27318178/identification-of-potential-targets-for-an-anticoagulant-pectin
#12
Aline Guimarães Santana, Ana Helena Pereira Gracher, André Luis Rüdiger, Nilson Ivo Tonin Zanchin, Paulo Costa Carvalho, Thales Ricardo Cipriani, Tatiana de Arruda Campos Brasil de Souza
: Heparin is a sulfated polysaccharide of animal origin showing excellent anticoagulant properties. Although it strongly inhibits the coagulation cascade, its interaction with multiple sites results in several side effects. An ideal alternative compound should not only possess anticoagulant and antithrombotic activities, but also provide specific binding to components of the coagulation cascade to decrease side effects and facilitate the control of pharmacologic actions in patient's body...
June 15, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27283236/characterization-and-functional-properties-of-mango-peel-pectin-extracted-by-ultrasound-assisted-citric-acid
#13
Miaomiao Wang, Bohui Huang, Chuanhui Fan, Kaili Zhao, Hao Hu, Xiaoyun Xu, Siyi Pan, Fengxia Liu
Pectin was extracted from 'Tainong No. 1' mango peels, using a chelating agent-citric acid as extraction medium by ultrasound-assisted extraction (UAE) and conventional extraction (CE) at temperatures of 20 and 80°C. Chemical structures, rheological and emulsifying properties of mango peel pectins (MPPs) were comparatively studied with laboratory grade citrus pectin (CP). All MPPs exhibited higher protein content (4.74%-5.94%), degree of methoxylation (85.43-88.38%), average molecular weight (Mw, 378.4-2858kDa) than the CP, but lower galacuronic acid content (GalA, 52...
October 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27238588/water-soluble-yellow-mustard-mucilage-a-novel-ingredient-with-potent-antioxidant-properties
#14
Y Wu, D Hui, N A M Eskin, S W Cui
The antioxidant properties of the water-soluble yellow mustard (Sinapis alba L.) mucilage (WSM) were compared with citrus pectin and xanthan gum using in vitro methods. The antioxidants ascorbic acid and butylated hydroxyanisole (BHA) were used as controls. The antioxidant activity, DPPH free radical scavenging ability, and reducing power on Fe were measured. Molecular weight (MW), uronic acid content, and viscosity for the three polysaccharides were obtained to investigate the relationships between the physicochemical properties and antioxidant activities of the three different polysaccharides...
October 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27234493/a-low-temperature-polygalacturonase-from-p-occitanis-characterization-and-application-in-juice-clarification
#15
Azza Hadj Sassi, Hajer Tounsi, Hèla Trigui-Lahiani, Rihab Bouzouita, Zamen Ben Romdhane, Ali Gargouri
An extracellular endo-polygalacturonase (PGase) was purified, after a single purification step, from the constitutive and hyperpectinolytic CT1 mutant of Penicillium occitanis. This enzyme named PG2 has a molecular weight of 42kDa. It was optimally active at 35°C and pH6 with more than 85% of activity at pH7 in contrast to the majority of fungal PGase, generally acting at 50°C and pH5. The specific activity obtained was among the highest ones, 31397.26U/mg. The PGase activity increased with the decrease of the degree of methylation (DM) of pectin, but it was also able to degrade the highly methyl-esterified substrates, 70% (DM) and 90% (DM), with almost 80% and 40% of residual activity respectively...
October 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27209035/expression-and-characterization-of-hyperthermostable-exo-polygalacturonase-ttgh28-from-thermotoga-thermophilus
#16
Kurt Wagschal, J Rose Stoller, Victor J Chan, Charles C Lee, Arabela A Grigorescu, Douglas B Jordan
D-galacturonic acid is a potential platform chemical comprising the principal component of pectin in the citrus processing waste stream. Several enzyme activities are required for the enzymatic production of galacturonic acid from pectin, including exo- and endo-polygalacturonases. The gene TtGH28 encoding a putative GH28 polygalacturonase from Pseudothermotoga thermarum DSM 5069 (Theth_0397, NCBI# AEH50492.1) was synthesized, expressed in Escherichia coli, and characterized. Alignment of the amino acid sequence of gene product TtGH28 with other GH28 proteins whose structures and details of their catalytic mechanism have been elucidated shows that three catalytic Asp residues and several other key active site residues are strictly conserved...
July 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/27194951/chemoprevention-of-low-molecular-weight-citrus-pectin-lcp-in-gastrointestinal-cancer-cells
#17
Shi Wang, Pei Li, Sheng-Min Lu, Zhi-Qiang Ling
BACKGROUND & AIMS: Low-molecular-weight citrus pectin (LCP) is a complex polysaccharide that displays abundant galactosyl (i.e., sugar carbohydrate) residues. In this study, we evaluated the anti-tumor properties of LCP that lead to Bcl-xL -mediated dampening of apoptosis in gastrointestinal cancer cells. METHODS: We used AGS gastric cancer and SW-480 colorectal cancer cells to elucidate the effects of LCP on cell viability, cell cycle and apoptosis in cultured cells and tumor xenografts...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27174558/effects-of-pectin-supplementation-on-the-fermentation-patterns-of-different-structural-carbohydrates-in-rats
#18
Lingmin Tian, Jan Scholte, Klaudyna Borewicz, Bartholomeus van den Bogert, Hauke Smidt, Anton J W Scheurink, Harry Gruppen, Henk A Schols
SCOPE: We aimed to investigate and compare the effects of four types of pectins on dietary fiber (DF) fermentation, microbiota composition and short chain fatty acid (SCFA) production throughout the large intestine in rats. METHODS AND RESULTS: Male Wistar rats were given diets supplemented with or without 3% structurally different pectins for seven weeks. Different fermentation patterns of pectins and different location of fermentation of pectin and diet arabinoxylans (AXs) in the large intestine were observed...
May 13, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27164497/anti-diabetic-effect-of-citrus-pectin-in-diabetic-rats-and-potential-mechanism-via-pi3k-akt-signaling-pathway
#19
Yanlong Liu, Man Dong, Ziyu Yang, Siyi Pan
This study was performed to investigate the anti-diabetic effect of citrus pectin in type 2 diabetic rats and its potential mechanism of action. The results showed that fasting blood glucose levels were significantly decreased after 4 weeks of citrus pectin administration. Citrus pectin improved glucose tolerance, hepatic glycogen content and blood lipid levels (TG, TC, LDL-c and HDL-c) in diabetic rats. Citrus pectin also significantly reduced insulin resistance, which played an important role in the resulting anti-diabetic effect...
August 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27129206/low-or-no-inhibitory-potency-of-the-canonical-galectin-carbohydrate-binding-site-by-pectins-and-galactomannans
#20
John Stegmayr, Adriana Lepur, Barbro Kahl-Knutson, Matilde Aguilar-Moncayo, Anatole A Klyosov, Robert A Field, Stina Oredsson, Ulf J Nilsson, Hakon Leffler
Some complex plant-derived polysaccharides, such as modified citrus pectins and galactomannans, have been shown to have promising anti-inflammatory and anti-cancer effects. Most reports propose or claim that these effects are due to interaction of the polysaccharides with galectins because the polysaccharides contain galactose-containing side chains that might bind this class of lectin. However, their direct binding to and/or inhibition of the evolutionarily conserved galactoside-binding site of galectins has not been demonstrated...
June 17, 2016: Journal of Biological Chemistry
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