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citrus pectin

Tian Fang, Dan-Dan Liu, He-Ming Ning, Dan Liu, Jing-Ya Sun, Xiao-Jing Huang, Yu Dong, Mei-Yu Geng, Shi-Feng Yun, Jun Yan, Rui-Min Huang
Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G2 /M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP...
May 16, 2018: Acta Pharmacologica Sinica
Karabi Roy, Sujan Dey, Md Kamal Uddin, Rasel Barua, Md Towhid Hossain
Pectinase is one of the important enzymes of industrial sectors. Presently, most of the pectinases are of plant origin but there are only a few reports on bacterial pectinases. The aim of the present study was to isolate a novel and potential pectinase producing bacterium as well as optimization of its various parameters for maximum enzyme production. A total of forty bacterial isolates were isolated from vegetable dump waste soil using standard plate count methods. Primary screening was done by hydrolysis of pectin...
2018: Enzyme Research
Cristiane Colodel, Lúcia Cristina Vriesmann, Reinaldo Francisco Teófilo, Carmen Lúcia de Oliveira Petkowicz
A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel. The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time. The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1...
May 10, 2018: International Journal of Biological Macromolecules
S H E Verkempinck, L Salvia-Trujillo, S Denis, A M Van Loey, M E Hendrickx, T Grauwet
Oil-in-water emulsions were prepared with 5% (w/v) carrot-enriched olive oil and stabilized with Tween 80 (TW), phosphatidylcholine (PC), citrus pectin (CP) or a combination of these emulsifiers. Additionally, the methylesterification degree (DM) of citrus pectin was modified, resulting in three different studied pectin structures: CP82, CP38 and CP10. All initial emulsions presented small initial oil droplet sizes and were submitted to an in vitro simulated gastric and small intestinal phase. The latter was executed in a kinetic way to determine the time dependency of the lipolysis reaction, micelle formation and carotenoid bioaccessibility...
October 1, 2018: Food Chemistry
Shan Qin Liew, Wen Hui Teoh, Chee Keong Tan, Rozita Yusoff, Gek Cheng Ngoh
Low methoxyl (LM) pectin was extracted from pomelo peels using subcritical water in a dynamic mode. The effects of pressure and temperature were analyzed through a face-centred central composite design. Extraction yield and the rate of extraction were found to be predominantly influenced by temperature. Optimization of the subcritical water extraction (SWE) yielded an optimized operating condition of 120 °C and 30 bar with a predicted pectin yield of 18.8%. The corresponding experimental yield was 19.6%, which is in close agreement with the predicted data...
May 5, 2018: International Journal of Biological Macromolecules
Xun Wang, Lijin Lin, Yi Tang, Hui Xia, Xiancong Zhang, Maolan Yue, Xia Qiu, Ke Xu, Zhihui Wang
BACKGROUND: During fresh fruit consumption, sensory texture is one factor that affects the organoleptic qualities. Chemical components of plant cell walls, including pectin, cellulose, hemicellulose and lignin, play central roles in determining the textural qualities. To explore the genes and regulatory pathways involved in fresh citrus' perceived sensory texture, we performed mRNA-seq analyses of the segment membranes of two citrus cultivars, Shiranui and Kiyomi, with different organoleptic textures...
April 23, 2018: BMC Genomics
Jaime Ibarrola, Vanessa Arrieta, Rafael Sádaba, Ernesto Martínez-Martínez, Amaia García-Peña, Virginia Alvarez, Amaya Fernández-Celis, Alicia Gainza, Enrique Santamaría, Joaquin Fernández-Irigoyen, Victoria Cachofeiro, Guillermo Zalba, Renaud Fay, Patrick Rossignol, Natalia López-Andrés
Galectin-3 (Gal-3) is increased in heart failure (HF) and promotes cardiac fibrosis and inflammation. We investigated whether Gal-3 modulates oxidative stress in human cardiac fibroblasts, in experimental animal models and in human aortic stenosis (AS). Using proteomics and immunodetection approaches, we have identified that Gal-3 down-regulated the anti-oxidant peroxiredoxin-4 (Prx-4) in cardiac fibroblasts. In parallel, Gal-3 increased peroxide, nitrotyrosine, malondialdehyde and N-carboxymethyl-lysine levels and decreased total antioxidant capacity...
April 19, 2018: Clinical Science (1979-)
Hua Zhang, Jianle Chen, Junhui Li, Chaoyang Wei, Xingqian Ye, John Shi, Shiguo Chen
Pectin had been recovered from canning wastewater produced by chemical treatment of segment membrane during preparation of canned citrus in our previous research. The purpose of this study was to characterize the extracted pectin from canning wastewater, and to evaluate its application as a fat alternative to replace fat in ice cream. The monosaccharide composition and rheological properties of the pectin were determined. The influences of fat reduction and pectin addition on the physicochemical, rheological and sensory properties of low-fat ice cream were determined...
April 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Navnidhi Chhikara, Ragni Kour, Sundeep Jaglan, Pawan Gupta, Yogesh Gat, Anil Panghal
Citrus medica (Citron) is an underutilized fruit plant having various bioactive components in all parts of the plant. The major bioactive compounds present are iso-limonene, citral, limonene, phenolics, flavonones, vitamin C, pectin, linalool, decanal, and nonanal, accounting for several health benefits. Pectin and heteropolysachharides also play a major role as dietary fibers. The potential impact of citron and its bioactive components to prevent or reverse destructive deregulated processes responsible for certain diseases has attracted different researchers' attention...
March 29, 2018: Food & Function
Mukesh Kumar Patidar, Sadhana Nighojkar, Anil Kumar, Anand Nighojkar
A plethora of solid substrates, cultivation conditions and enzyme assay methods have been used for efficient production and estimation of polygalacturonase and pectin methylesterase enzymes. Recent developments in industrial biotechnology offer several opportunities for the utilization of low cost agro-industrial waste in Solid State Fermentation (SSF) for the pectinolytic enzyme production using fungi. Fruit waste mainly citrus fruit waste alone and along with other agro-industrial waste has been explored in SSF for enzyme production...
April 2018: 3 Biotech
Tullio Venditti, Guy D'hallewin, Gianfranca Ladu, Giacomo L Petretto, Giorgio Pintore, John M Labavitch
BACKGROUND: The present study was performed to clarify the strategies of Penicillium digitatum during pathogenesis on citrus, assessing, on albedo plugs, the effects of treatment with NaHCO3 , at two different pH (5 and 8.3), on cell wall-degrading enzymes activity, over a period of 72 h. RESULTS: The treatment with NaHCO3 , under alkaline pH, delayed the polygalacturonase activity for 72 h, or 48 h in the case of the pectin lyase, if compared to the control or the same treatment at pH 5...
March 25, 2018: Journal of the Science of Food and Agriculture
Ana M Muñoz-Labrador, Rodrigo Moreno, Mar Villamiel, Antonia Montilla
BACKGROUND: Pectin is heteropolysaccharide found in cell walls coming mainly from by-products as well as citrus peels, apple and sugar beet pulp and presenting biological and techno-functional properties. In this work a generalproximal and structural characterisation of industrial citrus pectins was done together with a study of impact of power ultrasound (US) on their rheological properties with the aim of using them as edible coatings for fresh strawberries. RESULTS: The results indicated that pure pectin showed a methylesterification degree greater than 50% and galacturonic acid content > 65%, indicating its consideration as additive E-440, conditions that were not achieved by pectin with sugar addition...
March 23, 2018: Journal of the Science of Food and Agriculture
Dugald Seely, Julie K Ennis, Ellen McDonell, Linlu Zhao
BACKGROUND AND OBJECTIVES: There is a lack of information on therapies recommended by naturopathic doctors (NDs) for lung and gastroesophageal cancer care. Study objectives were to: (1) identify the most common interventions considered for use by NDs; (2) identify interventions NDs recommend to support key therapeutic goals; and (3) identify potential contraindications between integrative and conventional therapies. METHODS: Oncology Association of Naturopathic Physicians (OncANP) members (n = 351) were invited to complete an electronic survey...
March 1, 2018: Integrative Cancer Therapies
Ahmed I El-Batal, Nawal E Al-Hazmi, Farag M Mosallam, Gharieb S El-Sayyad
Mono-dispersed copper nanoparticles (CuNPs) were constructed using cheap polysaccharides (citrus pectin, chitosan, and sodium alginate), and by appropriating aqueous fermented fenugreek powder (FFP) under the action of Pleurotus ostreatus (as reducing and preserving means), through the influence of gamma irradiation. The synthesized CuNPs are described by UV-Vis. spectroscopy TEM, DLS, XRD, and FT-IR. XRD study of the CuNPs confirmed the generation of metallic CuNPs. The nucleation and the production mechanism of CuNPs are moreover explained...
March 9, 2018: Microbial Pathogenesis
Agnieszka Nawrocka, Magdalena Krekora, Zbigniew Niewiadomski, Antoni Miś
FTIR spectroscopy was used to determine changes in secondary structure, as well as water state, in gluten and model doughs supplemented by four fibre polysaccharides (microcrystalline cellulose, inulin, apple pectin and citrus pectin). The gluten and model doughs were obtained from commercially available wheat gluten and model flour, respectively. The polysaccharides were used in five concentrations: 3%, 6%, 9%, 12% and 18%. Analysis of the FTIR spectra indicated that polysaccharides could be divided into two groups: first - microcrystalline cellulose and inulin, second - apple and citrus pectins that induced opposite structural changes...
June 30, 2018: Food Chemistry
My-Nhan Nguyen, Yidan Su, Helen Kiriazis, Yan Yang, Xiao-Ming Gao, Julie R McMullen, Anthony M Dart, Xiao-Jun Du
Pre-clinical studies have demonstrated that anti-galectin-3 (Gal-3) interventions are effective in attenuating cardiac remodeling, fibrosis and dysfunction. We determined, in a transgenic (TG) mouse model of fibrotic cardiomyopathy, whether galectin-3 expression was elevated and whether Gal-3 played a critical role in disease development. We studied mice with fibrotic cardiomyopathy due to cardiac overexpression of human β2 -adrenoceptor (β2 -TG). Cardiac expression levels of Gal-3 and fibrotic or inflammatory genes were determined...
February 9, 2018: American Journal of Physiology. Heart and Circulatory Physiology
Gema Marín-Royo, Isabel Gallardo, Ernesto Martínez-Martínez, Beatriz Gutiérrez, Raquel Jurado-López, Natalia López-Andrés, Josué Gutiérrez-Tenorio, Eduardo Rial, Marı A Visitación Bartolomé, María Luisa Nieto, Victoria Cachofeiro
Obesity is accompanied by metabolic alterations characterized by insulin resistance and cardiac lipotoxicity. Galectin-3 (Gal-3) induces cardiac inflammation and fibrosis in the context of obesity; however, its role in the metabolic consequences of obesity is not totally established. We have investigated the potential role of Gal-3 in the cardiac metabolic disturbances associated with obesity. In addition, we have explored whether this participation is, at least partially, acting on mitochondrial damage. Gal-3 inhibition in rats that were fed a high-fat diet (HFD) for 6 weeks with modified citrus pectin (MCP; 100 mg/kg/day) attenuated the increase in cardiac levels of total triglyceride (TG)...
February 5, 2018: Disease Models & Mechanisms
Sibel Türkkan, Deniz Atila, Akın Akdağ, Ayşen Tezcaner
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnectivity with pore size about 120 µm that providing suitable microenvironment for cells...
January 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Dolores Mueller, Kathrin Jung, Manuel Winter, Dorothee Rogoll, Ralph Melcher, Ulrich Kulozik, Karin Schwarz, Elke Richling
Anthocyanins are flavonoids that have been suggested to provide beneficial health effects. The biological activity of anthocyanins is influenced by their pharmacokinetic properties, but anthocyanins are associated with limited bioavailability in humans. In the presented study, we investigated how the encapsulation of bilberry extract (BE), a source of anthocyanins, with either whey protein or citrus pectin influences the bioavailability and intestinal accessibility of anthocyanins in humans. We performed an intervention study that analyzed anthocyanins and their degradation products in the urine, plasma, and ileal effluent of healthy volunteers and ileostomists (subjects without an intact colon)...
May 15, 2018: Food Chemistry
Hiroshi Uno, Hirokazu Sekimoto, Tetsuro Kitamura
Pectin is used in several foods as an additive and a thickner. But some cases of anaphylaxis have been reported. Most of these are induced by occasional exposures; however, no cases of anaphylaxis after eating a Citrus unshiu, the albedo of which is rich in pectin, have been reported.A 7-year-old girl developed barking cough and pruritus approximately two hours after eating a frozen Citrus unshiu. She had a history of anaphylaxis induced by consuming cashew nuts. Skin testing and basophil activation tests were performed using a commercially available pectin product...
2017: Arerugī, [Allergy]
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