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citrus pectin

Dugald Seely, Julie K Ennis, Ellen McDonell, Linlu Zhao
BACKGROUND AND OBJECTIVES: There is a lack of information on therapies recommended by naturopathic doctors (NDs) for lung and gastroesophageal cancer care. Study objectives were to: (1) identify the most common interventions considered for use by NDs; (2) identify interventions NDs recommend to support key therapeutic goals; and (3) identify potential contraindications between integrative and conventional therapies. METHODS: Oncology Association of Naturopathic Physicians (OncANP) members (n = 351) were invited to complete an electronic survey...
March 1, 2018: Integrative Cancer Therapies
Ahmed I El-Batal, Nawal E Al-Hazmi, Farag M Mosallam, Gharieb S El-Sayyad
Mono-dispersed copper nanoparticles (CuNPs) were constructed using cheap polysaccharides (citrus pectin, chitosan, and sodium alginate), and by appropriating aqueous fermented fenugreek powder (FFP) under the action of Pleurotus ostreatus (as reducing and preserving means), through the influence of gamma irradiation. The created CuNPs are described by UV-Vis. spectroscopy TEM, DLS, XRD, and FT-IR. XRD study of the CuNPs confirmed the generation of metallic CuNPs. The nucleation and the production mechanism of CuNPs are moreover explained...
March 9, 2018: Microbial Pathogenesis
Agnieszka Nawrocka, Magdalena Krekora, Zbigniew Niewiadomski, Antoni Miś
FTIR spectroscopy was used to determine changes in secondary structure, as well as water state, in gluten and model doughs supplemented by four fibre polysaccharides (microcrystalline cellulose, inulin, apple pectin and citrus pectin). The gluten and model doughs were obtained from commercially available wheat gluten and model flour, respectively. The polysaccharides were used in five concentrations: 3%, 6%, 9%, 12% and 18%. Analysis of the FTIR spectra indicated that polysaccharides could be divided into two groups: first - microcrystalline cellulose and inulin, second - apple and citrus pectins that induced opposite structural changes...
June 30, 2018: Food Chemistry
My-Nhan Nguyen, Yidan Su, Helen Kiriazis, Yan Yang, Xiao-Ming Gao, Julie R McMullen, Anthony M Dart, Xiao-Jun Du
Pre-clinical studies have demonstrated that anti-galectin-3 (Gal-3) interventions are effective in attenuating cardiac remodeling, fibrosis and dysfunction. We determined, in a transgenic (TG) mouse model of fibrotic cardiomyopathy, whether galectin-3 expression was elevated and whether Gal-3 played a critical role in disease development. We studied mice with fibrotic cardiomyopathy due to cardiac overexpression of human β2 -adrenoceptor (β2 -TG). Cardiac expression levels of Gal-3 and fibrotic or inflammatory genes were determined...
February 9, 2018: American Journal of Physiology. Heart and Circulatory Physiology
Gema Marín-Royo, Isabel Gallardo, Ernesto Martínez-Martínez, Beatriz Gutiérrez, Raquel Jurado-López, Natalia López-Andrés, Josué Gutiérrez-Tenorio, Eduardo Rial, M V Bartolome, María Luisa Nieto, Victoria Cachofeiro
Obesity is accompanied by metabolic alterations characterized by insulin resistance and cardiac lipotoxicity. Galectin-3 (Gal-3) induces cardiac inflammation and fibrosis in the context of obesity; however, its role in the metabolic consequences of obesity is not totally established. We have investigated the potential role of Gal-3 in the cardiac metabolic disturbances associated with obesity. In addition, we have explored whether this participation is at least partially acting on mitochondrial damage. Gal-3 inhibition in rats fed a high-fat diet (HFD) for 6 weeks with modified citrus pectin (MCP; 100 mg/kg/day) attenuated the increase in cardiac levels of total triglyceride (TG)...
January 11, 2018: Disease Models & Mechanisms
Sibel Türkkan, Deniz Atila, Akın Akdağ, Ayşen Tezcaner
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnectivity with pore size about 120 µm that providing suitable microenvironment for cells...
January 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Dolores Mueller, Kathrin Jung, Manuel Winter, Dorothee Rogoll, Ralph Melcher, Ulrich Kulozik, Karin Schwarz, Elke Richling
Anthocyanins are flavonoids that have been suggested to provide beneficial health effects. The biological activity of anthocyanins is influenced by their pharmacokinetic properties, but anthocyanins are associated with limited bioavailability in humans. In the presented study, we investigated how the encapsulation of bilberry extract (BE), a source of anthocyanins, with either whey protein or citrus pectin influences the bioavailability and intestinal accessibility of anthocyanins in humans. We performed an intervention study that analyzed anthocyanins and their degradation products in the urine, plasma, and ileal effluent of healthy volunteers and ileostomists (subjects without an intact colon)...
May 15, 2018: Food Chemistry
Hiroshi Uno, Hirokazu Sekimoto, Tetsuro Kitamura
Pectin is used in several foods as an additive and a thickner. But some cases of anaphylaxis have been reported. Most of these are induced by occasional exposures; however, no cases of anaphylaxis after eating a Citrus unshiu, the albedo of which is rich in pectin, have been reported.A 7-year-old girl developed barking cough and pruritus approximately two hours after eating a frozen Citrus unshiu. She had a history of anaphylaxis induced by consuming cashew nuts. Skin testing and basophil activation tests were performed using a commercially available pectin product...
2017: Arerugī, [Allergy]
Fredrick Onyango Ogutu, Tai-Hua Mu, Hongnan Sun, Miao Zhang
BACKGROUND: Pectin and especially modified citrus pectin possesses anticancer activity. Hence, the current study investigated anticancer activity of ultrasonic-modified sweet potato pectin (SPP) on HT-29 cells to assess its potential as a cancer therapeutic agent. METHOD: The effect of ultrasonic treatment on SPP molecular weight, galacturonic acid content, degree of methoxylation, and neutral sugar was investigated. Moreover, the effect of sonicated variant on human HT-29 cell proliferation was assessed by MTT assay, cell cytotoxicity, and apoptosis by Annexin V/PI flow cytometer and caspase-3 activity was studied...
January 2018: Nutrition and Cancer
Xiuwen Wu, Muhammad Riaz, Lei Yan, Chenqing Du, Yalin Liu, Cuncang Jiang
Boron (B) is a micronutrient indispensable for citrus and B deficiency causes a considerable loss of productivity and quality in China. However, studies on pectin composition and architecture of cell wall components in trifoliate orange roots under B deficiency condition are not sufficient. In this study, we investigated the alteration in pectin characteristics and the architecture of cell wall components in trifoliate orange [ Poncirus trifoliata (L.) Raf.] roots under B starvation. The results showed that B-deficient roots resulted in a significant enlargement of root tips and an obvious decrease in cell wall B and uronic acid content in Na2 CO3 -soluble pectin compared with B-adequate roots...
2017: Frontiers in Plant Science
Aline Francielle Fracasso, Camila Augusto Perussello, Danielle Carpiné, Carmen Lúcia de Oliveira Petkowicz, Charles Windson Isidoro Haminiuk
The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin. Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties. The molar mass (Mw), degree of esterification (DE), monosaccharide composition, (13)C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated...
November 10, 2017: International Journal of Biological Macromolecules
Shanshan Zhang, Haijuan Hu, Lufeng Wang, Fengxia Liu, Siyi Pan
Pectin oligosaccharide (POS) fractions were obtained by controlled chemical degradation of citrus peel pectin. By adjusting trifluoroacetic acid (TFA) concentration, three oligosaccharides were obtained, of molecular weight (Mw) range 3000-4000 Da, 2000-3000 Da and lower than 2000 Da. Varying hydrogen peroxide (H2O2) concentration and reaction time produced oligosaccharides of 3000-4000 Da and 2000-3000 Da. The relative proportions of acidic monosaccharide increased from 68.58% to 89.93% (TFA) and from 63...
April 1, 2018: Food Chemistry
Jin-Shu Yang, Tai-Hua Mu, Meng-Mei Ma
Effects of HCl, H2SO4, HNO3, citric acid, and acetic acid on the yield, structure, and emulsifying properties of potato pectins were investigated. Results showed that the highest yield (14.34%) was obtained using citric acid, followed by HNO3 (9.83%), HCl (9.72%), H2SO4 (8.38%), and acetic acid (4.08%). The degrees of methylation (37.45%) and acetylation (15.38%), protein content (6.97%), and molecular weight (3.207 × 10(5) g/mol) were the highest for pectin extracted using acetic acid, and (galactose + arabinose)/rhamnose was 33...
April 1, 2018: Food Chemistry
Yuly A Ramírez-Tapias, Aldana S Lapasset Laumann, Claudia N Britos, Cintia W Rivero, Jorge A Trelles
Enzyme immobilization using hydrogels is a low-cost and effective system for the degradation of bulk pectin derived from orange industry residues. Polygalacturonases obtained from four different bacterial strains of Streptomyces genus were immobilized in alginate gel and assayed for pectin hydrolysis. The enzyme from Streptomyces halstedii ATCC 10897 proved to be superior and more stable within the alginate matrix. Furthermore, a new strategy to improve alginate bead stability using a mixture of calcium and strontium is reported; this technique allowed enhancing the mechanical properties by combining different amounts of these cations for ionotropic gelation...
December 2017: 3 Biotech
Ahmed I El-Batal, Farag M Mosalam, M M Ghorab, Amro Hanora, Ahmed M Elbarbary
Aqueous dispersed zinc nanoparticles (ZnNPs) were synthesized using natural polysaccherides (chitosan (CS), citrus pectin (CP) and alginate (Alg)) using aqueous fermented fenugreek powder (FFP) by Pleurotus ostreatus as reducing and stabilizing agent or using gamma irradiation. The synthesized ZnNPs are characterized by ultra violect spectroscopy (UV), Transmission electronmicroscopy (TEM), Dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). XRD analysis of the ZnNPS confirmed the formation of metallic nanoparticles...
October 30, 2017: International Journal of Biological Macromolecules
Bahare Pasandide, Faramarz Khodaiyan, Zeinab E Mousavi, Seyed Saeid Hosseini
In this study, the effect of aqueous extraction conditions (temperature of 70-90°C, time of 60-180min, and liquid/solid ratio (LSR) of 20-40v/w) on the yield and degree of esterification (DE) of Citrus medica peel pectin was studied using a Box-Behnken design. The highest production yield of pectin (21.85±0.35%) was obtained at temperature of 90°C, extraction time of 180min and LSR of 40v/w as optimum extraction conditions which was close to the predicted value (24.13%). In these extraction conditions, the DE and the emulsifying activity were 77...
December 15, 2017: Carbohydrate Polymers
Caroline C Kim, William J Kelly, Mark L Patchett, Gerald W Tannock, Z Jordens, Halina M Stoklosinski, Jordan W Taylor, Ian M Sims, Tracey J Bell, Douglas I Rosendale
A novel anaerobic pectinolytic bacterium (strain 14(T)) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14(T) belonged to the family Ruminococcaceae, but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14(T) was Acetivibrio cellulolyticus (89.7 % sequence similarity). Strain 14(T) shared ~99 % sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Keita Ishisono, Tomio Yabe, Kohji Kitaguchi
Pectin, a water-soluble dietary fiber, has been found to improve survival in endotoxin shock. However, the underlying mechanism by which pectin exerts its protective effect against endotoxin shock remains unknown. Apart from its prebiotic effects, it has been suggested that pectin directly affects immune cells to regulate inflammatory responses. In this study, we investigated the direct effect of pectin in murine model of endotoxin shock. Citrus pectin solution was administered to male C57BL/6 mice for 10 days...
August 12, 2017: Journal of Nutritional Biochemistry
Miriam G Shtriker, Michal Hahn, Elise Taieb, Abraham Nyska, Uzi Moallem, Oren Tirosh, Zecharia Madar
OBJECTIVE: Galactomannans derived from fenugreek confer known health benefits; however, there is little information regarding health benefits of citrus pectin (CP) and its association with gut microbiome metabolites. The aim of this study was to examine links between galactomannan and CP consumption, microbiota development, and glucose metabolism. DESIGN: Male C57 BL/6 J mice ages 7 to 8 wk were fed ad libitum with a normal diet or one supplemented with 15% of either galactomannan or CP...
August 3, 2017: Nutrition
Carlos-Ernesto Fernandez-García, Carlos Tarin, Raquel Roldan-Montero, Diego Martinez-Lopez, Monica Torres-Fonseca, Jes S Lindhot, Melina Vega de Ceniga, Jesus Egido, Natalia Lopez-Andres, Luis-Miguel Blanco-Colio, Jose-Luis Martín-Ventura
Abdominal aortic aneurysm (AAA) evolution is unpredictable and no specific treatment exists for AAA, except surgery to prevent aortic rupture. Galectin-3 has been previously associated with CVD, but its potential role in AAA has not been addressed. Galectin-3 levels were increased in the plasma of AAA patients (n=225) compared with the control group (n=100). In addition, galectin-3 concentrations were associated with the need for surgical repair, independently of potential confounding factors. Galectin-3 mRNA and protein expression were increased in human AAA samples compared with healthy aortas...
November 15, 2017: Clinical Science (1979-)
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