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citrus pectin

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https://www.readbyqxmd.com/read/28931282/crystallization-and-colloidal-stabilization-of-ca-oh-2-in-the-presence-of-nopal-juice-opuntia-ficus-indica-implications-in-architectural-heritage-conservation
#1
Carlos Rodriguez-Navarro, Encarnacion Ruiz-Agudo, Alejandro Burgos-Cara, Kerstin Elert, Eric F Hansen
Hydrated lime (Ca(OH)2) is a vernacular art and building material produced following slaking of CaO in water. If excess water is used, a slurry, called lime putty forms, which has been the preferred craftsman selection for formulating lime mortars since Roman times. A variety of natural additives were traditionally added to the lime putty to improve its quality. The mucilaginous juice extracted from nopal cladodes has been and still is used as additive incorporated in the slaking water for formulation of lime mortars and plasters both in ancient Mesoamerica as well as in the USA Southwest...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28911641/evaluation-of-the-prebiotic-effects-of-citrus-pectin-hydrolysate
#2
Yen-Yi Ho, Chia-Min Lin, Ming-Chang Wu
Citrus pectin enzyme hydrolysate (PEH) of different hydrolysis time intervals (6 hours, PEH-6; 12 hours, PEH-12; 24 hours, PEH-24; or 48 hours, PEH-48) or concentrations (1%, 2%, and 4%) was tested for its growth stimulation effect on two probiotics, Bifidobacterium bifidum and Lactobacillus acidophilus. Higher monosaccharide concentrations and smaller molecular weights of PEHs were obtained by prolonging the hydrolysis time. In addition, higher PEH concentrations resulted in significantly higher (p < 0...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28900464/synergistic-antioxidant-and-anti-inflammatory-effects-between-modified-citrus-pectin-and-honokiol
#3
Cheppail Ramachandran, Barry Wilk, Steven J Melnick, Isaac Eliaz
Inflammation is a normal physiological process; however, dysregulation of this process may contribute to inflammatory-based chronic disorders and diseases in animals and humans. Therefore, the antioxidant and anti-inflammatory properties of natural products, often recognized in traditional medicine systems, represent therapeutic modalities to reduce or prevent uncontrolled inflammatory processes which in turn potentially ameliorate or prevent sequelae of inflammatory-based symptoms of chronic diseases. We have investigated the antioxidant and anti-inflammatory effects of honokiol (HNK) and modified citrus pectin (MCP) in vitro and examined whether the MCP : HNK combination has synergistic effects on antioxidant and anti-inflammatory properties...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28888433/apple-pectin-derived-oligosaccharides-produce-carbon-dioxide-radical-anion-in-fenton-reaction-and-prevent-growth-of-escherichia-coli-and-staphylococcus-aureus
#4
Jelena Martinov, Miodrag Krstić, Snežana Spasić, Srdjan Miletić, Jovana Stefanović-Kojić, Aleksandra Nikolić-Kokić, Duško Blagojević, Ivan Spasojević, Mihajlo B Spasić
Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate)...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28858976/galectin-3-pharmacological-inhibition-attenuates-early-renal-damage-in-spontaneously-hypertensive-rats
#5
Ernesto Martínez-Martínez, Jaime Ibarrola, Amaya Fernández-Celis, Laurent Calvier, Celine Leroy, Victoria Cachofeiro, Patrick Rossignol, Natalia López-Andrés
BACKGROUND: The pharmacological blockade of galectin-3 (Gal-3), a β-galactoside-binding lectin, reduces renal impairment in acute kidney injury, hyperaldosteronism or nephropathy. We herein investigated the effects of pharmacological Gal-3 inhibition by modified citrus pectin (MCP) in renal damage in spontaneously hypertensive rats (SHRs). METHODS AND RESULTS: Gal-3 inhibition did not modify blood pressure levels in 30-week-old SHR. Kidney weight was higher in SHR, with no effect of MCP treatment (100 mg/kg/day in the drinking water)...
August 30, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28843267/screening-for-circulating-tumour-cells-allows-early-detection-of-cancer-and-monitoring-of-treatment-effectiveness-an-observational-study
#6
Karin Ried, Peter Eng, Avni Sali
Background: Circulating-Tumour-Cells (CTC) provide a blood biomarker for early carcinogenesis, cancer progression and treatment effectiveness. An increase in CTCs is associated with cancer progression, a CTC decrease with cancer containment or remission. Several technologies have been developed to identify CTC, including the validated Isolation-by-Size-of-Epithelial-Tumour (ISET, Rarecells) technology, combining blood filtration and microscopy using standard histo-pathological criteria. Methods: This observational study compared CTC count to cancer status and cancer risk, by monitoring treatment effectiveness in cancer patients and by screening for CTC in asymptomatic patients with risk factors, including family history of cancer...
August 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28828292/characterization-and-molecular-modeling-of-polygalacturonase-isoforms-from-saccharomyces-cerevisiae
#7
Vijayakumar Poondla, Rajasekhar Chikati, Monika Kallubai, Vidyasagar Chennupati, Rajagopal Subramanyam, Vijaya Sarathi Reddy Obulam
Earlier, low-temperature-active polygalacturonase isoforms from Saccharomyces cerevisiae PVK4 were isolated and purified. Substrate specificity of polygalacturonase isoforms indicated high affinity for pectins and very low enzyme activity towards non-pectic polysaccharides. To characterize the polygalacturonase isoforms, biochemical, spectral, and in silico approaches were used. The apparent Km and Vmax values for hydrolysis of pectin and galacturonic acid were 0.31 mg/ml and 3.15 mmol min/mg, respectively...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28821126/anticoagulant-and-antithrombotic-effects-of-chemically-sulfated-fucogalactan-and-citrus-pectin
#8
Yony Román, Helyn P de Oliveira Barddal, Marcello Iacomini, Guilherme L Sassaki, Thales R Cipriani
Citrus pectin (CP14) from Citrus sinensis, and a fucogalactan (E) and a glucan (G16) from Agaricus bisporus were isolated and structurally characterized. CP14 was constituted by (1→4)-linked α-d-GalpA units, E was composed by a (1→6)-linked α-d-Galp main-chain, partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp, and partially methylated at O-3, whereas G16 was composed of (1→6)-linked β-d-Glcp units. The polysaccharides were sulfated giving rise to CP14S, ESL and G16S...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821065/physicochemical-and-powder-characteristics-of-various-citrus-pectins-and-their-application-for-oral-pharmaceutical-tablets
#9
Parichat Chomto, Jurairat Nunthanid
The physicochemical and powder properties of citrus pectins varying in molecular weight and degree of esterification (DE) were characterized. All the pectins were flake particles with average sizes of around 84-107μm in diameter. They were amorphous solids and classified as being from slightly to moderately hygroscopic. Pomelo pectin possessed passable to poor flowability while the others were classified as having fair flowability. Heckel's analysis indicated that all pectins underwent plastic deformation under compression...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28818729/characterization-of-citrus-pectin-films-integrated-with-clove-bud-essential-oil-physical-thermal-barrier-antioxidant-and-antibacterial-properties
#10
Tanzeela Nisar, Zi-Chao Wang, Xi Yang, You Tian, Muneeb Iqbal, Yurong Guo
The increasing demand for bio-based materials to be used in food packaging has stimulated the development of novel, environmentally-friendly edible films. Antimicrobial films were developed by incorporating different levels of clove bud essential oil (0.5%, 1.0%, and 1.5%) into the citrus pectin in order to modify the functional properties of the films. Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry analysis (DSC) and X-ray diffraction (XRD) were performed, together with the determination of physical, optical, mechanical, antioxidant and antimicrobial properties of pectin emulsified films...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28758988/beneficial-effects-of-galectin-3-blockade-in-vascular-and-aortic-valve-alterations-in-an-experimental-pressure-overload-model
#11
Jaime Ibarrola, Ernesto Martínez-Martínez, J Rafael Sádaba, Vanessa Arrieta, Amaia García-Peña, Virginia Álvarez, Amaya Fernández-Celis, Alicia Gainza, Patrick Rossignol, Victoria Cachofeiro Ramos, Natalia López-Andrés
Galectin-3 (Gal-3) is involved in cardiovascular fibrosis and aortic valve (AV) calcification. We hypothesized that Gal-3 pharmacological inhibition with modified citrus pectin (MCP) could reduce aortic and AV remodeling in normotensive rats with pressure overload (PO). Six weeks after aortic constriction, vascular Gal-3 expression was up-regulated in male Wistar rats. Gal-3 overexpression was accompanied by an increase in the aortic media layer thickness, enhanced total collagen, and augmented expression of fibrotic mediators...
July 31, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28718883/gelation-kinetics-and-characterization-of-enzymatically-enhanced-fish-scales-gelatin-pectin-coacervate
#12
Tao Huang, Zong-Cai Tu, Xinchen Shangguan, Hui Wang, Hainan-Zhang, Lu Zhang, Xiaomei Sha
BACKGROUND: Protein - polysaccharide complex coacervations have been considered extensively for the development of functional foods. The main problem of the complex coacervates is high unstable under different conditions and that cross-linking is necessary to stabilize them. In this study, the effects of pectin at different concentrations on the gel and structural properties of fish scales gelatin (FSG) - high methoxyl citrus pectin (HMP) coacervate enhanced by microbial-transglutanminase (MTGase) were studied...
July 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28665303/citrus-pectin-derived-carbon-microspheres-with-superior-adsorption-ability-for-methylene-blue
#13
Wenlin Zhang, Zhiqin Zhou
In this study, citrus pectin-derived, green, and tunable carbon microspheres with superior adsorption capacity and high adsorption rate, as well as good reusability toward methylene blue adsorption, were prepared by a facile hydrothermal method without any hazardous chemicals. The materials hold great potential for the treatment of methylene blue wastewater.
June 30, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28648174/functionalization-of-pectin-with-laccase-mediated-oxidation-products-of-ferulic-acid
#14
N Karaki, A Aljawish, L Muniglia, S Bouguet-Bonnet, S Leclerc, C Paris, J Jasniewski, C Humeau-Virot
Pectin is a natural biopolymer extracted mostly from citrus peel, sugar beet and apple pomace. In order to improve its functional properties and then to enlarge the field of its potential applications, functionalization reaction of citrus pectin with ferulic acid (FA)-oxidation products was performed in aqueous medium, at 30°C and pH7.5, in the presence of Myceliophthora thermophila laccase as biocatalyst. The conjugation between FA-oxidation products and pectin was confirmed using FTIR, UV-Vis and LC-MS analyses...
September 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28630702/evaluation-of-an-in-vitro-fibre-fermentation-method-using-feline-faecal-inocula-repeatability-and-reproducibility
#15
Guido Bosch, Lisa Heesen, Karine de Melo Santos, Wilbert F Pellikaan, John W Cone, Wouter H Hendriks
To gain knowledge on the precision of an in vitro method for characterisation of the fermentability of dietary fibres, this study aimed to evaluate the repeatability and reproducibility of such a method. Substrates used were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from five cats with three replicates for each substrate-cat combination. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) were determined after incubation...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/28630701/evaluation-of-an-in-vitro-fibre-fermentation-method-using-feline-faecal-inocula-inter-individual-variation
#16
Guido Bosch, Lisa Heesen, Karine de Melo Santos, John W Cone, Wilbert F Pellikaan, Wouter H Hendriks
The present study aimed to evaluate the inter-individual variability in fermentation of standard fibrous substrates by faecal inocula from ten healthy adult female cats. Substrates were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from each cat. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) after incubation. Out of ten cats, nine produced faeces on the days of inoculum preparation...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/28585703/husk-tomato-physalis-ixocarpa-brot-waste-as-a-promising-source-of-pectin-extraction-and-physicochemical-characterization
#17
Blanca Elizabeth Morales-Contreras, Juan Carlos Contreras-Esquivel, Louise Wicker, Luz Araceli Ochoa-Martínez, Juliana Morales-Castro
Husk tomato (Physalis ixocarpa Brot. var. Rendidora) waste was evaluated as a source of specialized pectin, and pectin extracted from this waste was characterized physicochemically. Fruit was blanched for 10 or 15 min and extracted in 0.1 N HCl for 15 to 25 min. Extracted pectin was subjected to physicochemical analysis. For all extraction conditions, the percentage of anhydrogalacturonic acid exceeded 60%, indicating that husk tomato was a good source of pectin. The degree of esterification of pectin molecules was 63% to 91%...
July 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28560429/modified-citrus-pectin-inhibits-galectin-3-function-to-reduce-atherosclerotic-lesions-in-apoe-deficient-mice
#18
Yonggang Lu, Mingming Zhang, Pei Zhao, Min Jia, Bing Liu, Qian Jia, Jun Guo, Lin Dou, Jian Li
Galectin-3 is a carbohydrate-binding lectin, which has been implicated in the modulation of atherosclerotic pathophysiology, and is highly expressed in monocytes, macrophages and endothelial cells within atherosclerotic plaques. Modified citrus pectin (MCP) is produced from citrus pectin via pH and temperature modifications, which break it into shorter, non‑branched, galactose‑rich carbohydrate chains. MCP is able to tightly bind with galectin‑3, via recognition of its carbohydrate recognition domain, and facilitates the modulation of galectin‑3‑induced bioactivity...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28555295/expression-and-characterization-of-hyperthermostable-exopolygalacturonase-rmgh28-from-rhodothermus-marinus
#19
Kurt C Wagschal, J Rose Stoller, Victor J Chan, Douglas B Jordan
The gene RmGH28 from the organism Rhodothermus marinus, a putative glycosyl hydrolase family 28 polygalacturonase, was expressed in Escherichia coli and biochemically characterized. The gene was found to encode an exopolygalacturonase termed RmGH28, with galacturonic acid monomer and the polymer substrate (n-1) as the products released when acting on de-esterified polygalacturonic acid from citrus pectin. The enzyme at 25 °C had k cat ∼6 s(-1) when acting on polygalacturonic acid, with K m ∼0.7 μM and a substrate inhibition constant K si ∼70 μM...
May 29, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28534166/in-silico-identification-and-validation-of-potential-micrornas-in-kinnow-mandarin-citrus-reticulata-blanco
#20
Prashant Mohanpuria, Naveen Duhan, Navraj Kaur Sarao, Manvir Kaur, Mandip Kaur
MicroRNAs (miRNAs) are a large family of 19-25 nucleotides, regulatory, non-coding RNA molecules that control gene expression by cleaving or inhibiting the translation of target gene transcripts in animals and plants. Despite the important functions of miRNAs related to regulation of plant growth and development processes, metabolism, and abiotic and biotic stresses, little is known about the disease-related miRNA. Here, we present a new pipeline for miRNA analysis using expressed sequence tags (ESTs)-based bioinformatics approach in Kinnow mandarin, a commercially important citrus fruit crop...
May 22, 2017: Interdisciplinary Sciences, Computational Life Sciences
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