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https://www.readbyqxmd.com/read/29345333/effects-and-mechanism-of-puerarin-on-the-human-retinoblastoma-cells
#1
Chao Yang, Xiaohui Fan, Shuxia Fan
Puerarin is an isoflavonoid that is extracted from Kudzu root and is considered to have an anti-tumor effect. In the present study, the effects of puerarin on human retinoblastoma cells and the related pathways was determined. The retinoblastoma (RB) cell lines were used in this study. Cell viability and proliferation was measured by MTT assay. Cell cycle and apoptosis was determined by flow cytometry. The expression of caspase-3 and apoptosis-related proteins were detected by Western blotting following puerarin treatment...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29244731/comparative-therapeutic-effects-of-plant-extract-synthesized-and-traditionally-synthesized-gold-nanoparticles-on-alcohol-induced-inflammatory-activity-in-sh-sy5y-cells-in-vitro
#2
Ashok K Singh
The present study describes potential beneficial and adverse effects of plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y cells. Although kudzu root extract (K), edible-gum extract (G), alone or in combination (KG), reduced Au3+ into AuNPs, the extract's composition and the reaction temperature determined their size (AuNPKG(90<50<37) < AuNPK(90,50<37) < AuNPG(90<50); the subscript KG, K, or G is extract identification and numerical vales are reaction temperature in Celsius) and biological properties (AuNPKG(90,50>37) < AuNPK(90,50>37) < AuNPG(90,50))...
December 15, 2017: Biomedicines
https://www.readbyqxmd.com/read/29223058/discrimination-of-geographical-origin-and-detection-of-adulteration-of-kudzu-root-by-fluorescence-spectroscopy-coupled-with-multi-way-pattern-recognition
#3
Leqian Hu, Shuai Ma, Chunling Yin
In this work, fluorescence spectroscopy combined with multi-way pattern recognition techniques were developed for determining the geographical origin of kudzu root and detection and quantification of adulterants in kudzu root. Excitation-emission (EEM) spectra were obtained for 150 pure kudzu root samples of different geographical origins and 150 fake kudzu roots with different adulteration proportions by recording emission from 330 to 570nm with excitation in the range of 320-480nm, respectively. Multi-way principal components analysis (M-PCA) and multilinear partial least squares discriminant analysis (N-PLS-DA) methods were used to decompose the excitation-emission matrices datasets...
December 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29218115/erratum-preventive-effects-of-kudzu-root-on-bone-loss-and-cartilage-degradation-in-ovariectomized-rats
#4
Yunyun Luo, Shuang Zheng, Yujia Ding, Yueqin Dai, Yi Zhou, Ruifeng Xiang, Anne C Bay-Jensen, Morten A Karsdal, Per Qvist, Qinlong Zheng
[This corrects the article on p. 3517 in vol. 9, PMID: 28804568.].
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29029301/attractiveness-of-host-plants-at-different-growth-stage-to-kudzu-bug-megacopta-cribraria-heteroptera-plataspidae-behavioral-responses-to-whole-plant-and-constitutive-volatiles
#5
L Yang, X P Hu, E van Santen, X N Zeng
The kudzu bug, Megacopta cribraria (Fabricius), is an invasive pest of soybeans, Glycine max (L.) Merr., that has recently been detected in the United States. This study investigated whether there was a differential attraction of adult bugs to soybean growth stages, and whether the attraction was related to soybean constitutive volatiles. Greenhouse choice assays examined the behavioral orientation preference of adult bugs exposed to four growth stages of whole soybean plants: vegetative (V2), flowering (R1), pod (R3), and seed (R5)...
September 27, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29028996/cold-tolerance-of-megacopta-cribraria-hemiptera-plataspidae-an-invasive-pest-of-soybeans
#6
Jessica I Grant, William O Lamp
Kudzu bug, Megacopta cribraria Fabricius (Hemiptera: Plataspidae), first discovered in the United States in 2009, is an invasive pest of soybeans. From 2013 to 2016, Maryland has been the northern limit of its distribution in the United States. We sought to determine the physiological cold temperature limits, timing of movement to overwintering locations, and to characterize overwintering microhabitat temperature. We measured supercooling point (SCP) on three populations from distinct USDA plant hardiness zones in Maryland and Virginia between October and December of 2015...
September 23, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/29028992/spatial-distribution-of-megacopta-cribraria-hemiptera-plataspidae-adults-eggs-and-parasitism-by-paratelenomus-saccharalis-hymenoptera-platygastridae-in-soybean
#7
Ian A Knight, Phillip M Roberts, Wayne A Gardner, Kerry M Oliver, Francis P F Reay-Jones, Dominic D Reisig, Michael D Toews
Since 2014, populations of the kudzu bug, Megacopta cribraria (F.) (Hemiptera: Plataspidae), have declined in the southeastern United States and seldom require treatment. This decline follows the discovery of Paratelenomus saccharalis (Dodd; Hymenoptera: Platygastridae), a non-native egg parasitoid. The objective of this project was to observe the temporal and spatial dynamics of P. saccharalis parasitism of kudzu bug egg masses in commercial soybean fields. Four fields were sampled weekly for kudzu bugs and egg masses at a density of one sample per 0...
October 9, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28927631/formulation-and-characterization-of-o-w-emulsions-stabilized-using-octenyl-succinic-anhydride-modified-kudzu-starch
#8
Yiguo Zhao, Nauman Khalid, Gaofeng Shu, Marcos A Neves, Isao Kobayashi, Mitsutoshi Nakajima
Kudzu starch esterified with octenyl succinic anhydride (OSA) was used as a food-grade emulsifier to formulate O/W emulsions. In addition, the difference between the physicochemical properties and emulsifying ability of native kudzu starch and those of OSA-modified kudzu starch was investigated. Granules of the OSA-modified kudzu starches increased in size after gelatinization. The interfacial tension between soybean oil and gelatinized OSA-modified kudzu starch was lower than that of kudzu starch. The droplet size of O/W emulsions decreased to 186nm at 100MPa after three passes...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28898994/how-well-do-growing-season-dynamics-of-photosynthetic-capacity-correlate-with-leaf-biochemistry-and-climate-fluctuations
#9
Danielle A Way, Joseph R Stinziano, Henry Berghoff, Ram Oren
Accurate values of photosynthetic capacity are needed in Earth System Models to predict gross primary productivity. Seasonal changes in photosynthetic capacity in these models are primarily driven by temperature, but recent work has suggested that photoperiod may be a better predictor of seasonal photosynthetic capacity. Using field-grown kudzu (Pueraria lobata (Willd.) Ohwi), a nitrogen-fixing vine species, we took weekly measurements of photosynthetic capacity, leaf nitrogen, and pigment and photosynthetic protein concentrations and correlated these with temperature, irradiance and photoperiod over the growing season...
July 1, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28830209/puerarin-improves-vascular-insulin-resistance-and-cardiovascular-remodeling-in-salt-sensitive-hypertension
#10
Chunxiang Tan, Aimei Wang, Chan Liu, Yao Li, Yuepin Shi, Ming-Sheng Zhou
Puerarin is an isoflavonoid isolated from the Chinese herb, Kudzu roots (also known as Gegen), which has been widely used for the treatment of hypertensive diseases and diabetic mellitus in traditional Chinese medicine. Dahl salt-sensitive (DS) rat is a genetic model of salt-sensitive hypertension with cardiovascular injury and vascular insulin resistance. Here, we investigated whether puerarin improved vascular insulin resistance and attenuated cardiac and aortic remodeling in salt-sensitive hypertension. DS rats were given a normal (NS) or high salt diet (HS) for five weeks...
2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28807875/bioactive-isoflavones-from-pueraria-lobata-root-and-starch-different-extraction-techniques-and-carbonic-anhydrase-inhibition
#11
Andrei Mocan, Simone Carradori, Marcello Locatelli, Daniela Secci, Stefania Cesa, Adriano Mollica, Simona Riga, Andrea Angeli, Claudiu T Supuran, Christian Celia, Luisa Di Marzio
Kudzu, the dried root of an important edible plant (Pueraria lobata), is used in Traditional Chinese Medicine for the important nutritional value strictly related to its isoflavone derivatives. These compounds characterize the quality of kudzu contained in different preparations, as pharmaceutical ingredient as well as dietary/food supplement (e.g. starch). The optimization of the isoflavones recovery, monitored by HPLC-PDA, through different innovative and conventional extraction techniques, e.g. microwave-assisted, ultrasound-assisted and conventional extraction, represented a suitable challenge in food industry and natural products evaluation...
August 11, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28804568/preventive-effects-of-kudzu-root-on-bone-loss-and-cartilage-degradation-in-ovariectomized-rat
#12
Yunyun Luo, Shuang Zheng, Yujia Ding, Yueqin Dai, Yi Zhou, Ruifeng Xiang, Anne C Bay-Jensen, Morten A Karsdal, Per Qvist, Qinlong Zheng
The clinical utility of Traditional Chinese Medicine (TCM) herbs/roots extracts in osteoporosis (OP) and osteoarthritis (OA) has been described in multiple reports, but there have been few studies of TCM for preventing bone loss and cartilage degradation simultaneously. Six-month-old female Sprague-Dawley rats each were subjected to ovariectomized (OVX) or sham surgery and treated orally once daily with herbal extracts or vehicle. Body weight was recorded weekly, and blood samples were collected from fasting animals at different time points...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28791618/boosting-isoprene-production-via-heterologous-expression-of-the-kudzu-isoprene-synthase-gene-kisps-into-bacillus-spp-cell-factory
#13
Lamis Gomaa, Michael E Loscar, Haggag S Zein, Nahed Abdel-Ghaffar, Abdelhadi A Abdelhadi, Ali S Abdelaal, Naglaa A Abdallah
Isoprene represents a key building block for the production of valuable materials such as latex, synthetic rubber or pharmaceutical precursors and serves as basis for advanced biofuel production. To enhance the production of the volatile natural hydrocarbon isoprene, released by plants, animals and bacteria, the Kudzu isoprene synthase (kIspS) gene has been heterologously expressed in Bacillus subtilis DSM 402 and Bacillus licheniformis DSM 13 using the pHT01 vector. As control, the heterologous expression of KIspS in E...
August 8, 2017: AMB Express
https://www.readbyqxmd.com/read/28705501/morphology-crystallinity-pasting-thermal-and-quality-characteristics-of-starches-from-adzuki-bean-vigna-angularis-l-and-edible-kudzu-pueraria-thomsonii-benth
#14
Chagam Koteswara Reddy, Fei Luan, Baojun Xu
Starches were isolated from adzuki bean (Vigna angularis L.) and edible kudzu (Pueraria thomsonii Benth) and investigated for their physico-chemical, morphological, pasting, crystallinity, thermal and FT-IR spectroscopic characteristics. Statistical analysis of physico-chemical and functional characteristics showed significant (p<0.05) difference between isolated starches from adzuki bean and edible kudzu. The XRD pattern of starches from adzuki bean and edible kudzu showed A-type with reflections (2θ) at 15...
December 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28638404/puerarin-protects-against-cardiac-fibrosis-associated-with-the-inhibition-of-tgf-%C3%AE-1-smad2-mediated-endothelial-to-mesenchymal-transition
#15
Ya-Ge Jin, Yuan Yuan, Qing-Qing Wu, Ning Zhang, Di Fan, Yan Che, Zhao-Peng Wang, Yang Xiao, Sha-Sha Wang, Qi-Zhu Tang
BACKGROUND: Puerarin is a kind of flavonoids and is extracted from Chinese herb Kudzu root. Puerarin is widely used as an adjuvant therapy in Chinese clinics. But little is known about its effects on regulating cardiac fibrosis. METHODS: Mice were subjected to transverse aorta constriction (TAC) for 8 weeks; meanwhile puerarin was given 1 week after TAC. Cardiac fibrosis was assessed by pathological staining. The mRNA and protein changes of CD31 and vimentin in both animal and human umbilical vein endothelial cells (HUVECs) models were detected...
2017: PPAR Research
https://www.readbyqxmd.com/read/28583762/puerarin-inhibits-amyloid-%C3%AE-induced-nlrp3-inflammasome-activation-in-retinal-pigment-epithelial-cells-via-suppressing-ros-dependent-oxidative-and-endoplasmic-reticulum-stresses
#16
Ke Wang, Xue Zhu, Kai Zhang, Yong Yao, Miao Zhuang, Chengye Tan, Fanfan Zhou, Ling Zhu
Amyloid β (Aβ) is a critical stimulator that promotes the progression of age-related macular degeneration (AMD). NLRP3 inflammasome activation induced by Aβ is estimated to be responsible for retinal pigment epithelium (RPE) dysfunction in such disease. Puerarin, one of the major active constituents of Kudzu root, has been widely used in the clinical treatment of AMD in China for decades; however, the detailed molecular mechanism remains far from clear. In this study, we investigated the protective effect and underlying mechanism of puerarin against Aβ1-40-induced NLRP3 inflammasome activation in LPS-primed ARPE-19 cells...
August 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28560744/silage-preparation-and-fermentation-quality-of-kudzu-sugarcane-top-and-their-mixture-treated-with-lactic-acid-bacteria-molasses-and-cellulase
#17
Xinzhu Chen, Wenyang Li, Chengfang Gao, Xiaopei Zhang, Boqi Weng, Yimin Cai
We studied silage fermentation of kudzu (KZ), sugarcane top (ST) and their mixtures treated with additives to be able to effectively use available local feed resources. The silages were prepared using KZ, ST, KZ 90% + ST 10%, KZ 80% + ST 20%, KZ 70% + ST 30% and KZ 60% + ST 40%, based on fresh matter (FM). These silages were treated with 10(8)  colony-forming units/g epiphytic lactic acid bacteria (LAB), 5% molasses and 0.02% cellulase of FM. The KZ contained higher crude protein (CP; 14.52%) content and lower levels of neutral detergent fiber (NDF; 62...
May 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28480539/plant-litter-chemistry-alters-the-content-and-composition-of-organic-carbon-associated-with-soil-mineral-and-aggregate-fractions-in-invaded-ecosystems
#18
Mioko Tamura, Vidya Suseela, Myrna Simpson, Brian Powell, Nishanth Tharayil
Through the input of disproportionate quantities of chemically distinct litter, invasive plants may potentially influence the fate of organic matter associated with soil mineral and aggregate fractions in some of the ecosystems they invade. Although context dependent, these native ecosystems subjected to prolonged invasion by exotic plants may be instrumental in distinguishing the role of plant-microbe-mineral interactions from the broader edaphic and climatic influences on the formation of soil organic matter (SOM)...
October 2017: Global Change Biology
https://www.readbyqxmd.com/read/28407550/potential-phosphorus-eutrophication-mitigation-strategy-biochar-carbon-composition-thermal-stability-and-ph-influence-phosphorus-sorption
#19
L W Ngatia, Y P Hsieh, D Nemours, R Fu, R W Taylor
Phosphorus (P) eutrophication is a major pollution problem globally, with unprecedented amount of P emanating from agricultural sources. But little is known about the optimization of soil-biochar P sorption capacity. The study objective was to determine how biochar feedstocks and pyrolysis conditions influences carbon (C) thermal stability, C composition and pH and in turn influence the phosphorus sorption optimization. Biochar was produced from switchgrass, kudzu and Chinese tallow at 200, 300, 400, 500, 550, 650,750 °C...
August 2017: Chemosphere
https://www.readbyqxmd.com/read/28400805/3-daidzein-sulfonate-sodium-improves-mitochondrial-functions-after-cerebral-ischemia-reperfusion-injury
#20
Wa Yuan, Qin Chen, Jing Zeng, Hai Xiao, Zhi-Hua Huang, Xiao Li, Qiong Lei
3'-Daidzein sulfonate sodium is a new synthetic water-soluble compound derived from daidzein (an active ingredient of the kudzu vine root). It has been shown to have a protective effect on cerebral ischemia/reperfusion injury in rats. We plan to study the mechanism of its protective effect. 3'-Daidzein sulfonate sodium was injected in rats after cerebral ischemia/reperfusion injury. Results showed that 3'-daidzein sulfonate sodium significantly reduced mitochondrial swelling, significantly elevated the mitochondrial membrane potential, increased mitochondrial superoxide dismutase and glutathione peroxidase activities, and decreased mitochondrial malondialdehyde levels...
February 2017: Neural Regeneration Research
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