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https://www.readbyqxmd.com/read/28154486/viral-effects-of-a-dsrna-mycovirus-pov-asi2792-on-the-vegetative-growth-of-the-edible-mushroom-pleurotus-ostreatus
#1
Ha-Yeon Song, Hyo-Jin Choi, Hansaem Jeong, Dahye Choi, Dae-Hyuk Kim, Jung-Mi Kim
A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P...
December 2016: Mycobiology
https://www.readbyqxmd.com/read/28149172/yield-size-nutritional-value-and-antioxidant-activity-of-oyster-mushrooms-grown-on-perilla-stalks
#2
Huizhen Li, Zhijun Zhang, Mengxue Li, Xiaojun Li, Ziwen Sun
Perilla is an edible medical plant with rapidly increasing acreage in China. In this study, we investigated the potential of perilla stalks (PSs) as an alternative substrate for the cultivation of oyster mushrooms (Pleurotus ostreatus). P. ostreatus was cultivated on cottonseed hulls (CSH) alone or mixed with PSs in different ratios. The production parameters, physical characteristics, nutritional values, and antioxidant activity of mushrooms cultivated on different substrate mixtures were determined. The addition of PSs to CSH significantly improved the growth rate, yield, biological efficiency, and proximate composition and shortened the cultivation cycle...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28086773/pleurotus-giganteus-berk-karun-hyde-the-giant-oyster-mushroom-inhibits-no-production-in-lps-h2o2-stimulated-raw-264-7-cells-via-stat-3-and-cox-2-pathways
#3
Asweni Baskaran, Kek Heng Chua, Vikineswary Sabaratnam, Mani Ravishankar Ram, Umah Rani Kuppusamy
BACKGROUND: Pleurotus giganteus (Berk. Karunarathna and K.D. Hyde), has been used as a culinary mushroom and is known to have medicinal properties but its potential as an anti-inflammatory agent to mitigate inflammation triggered diseases is untapped. In this study, the molecular mechanism underlying the protective effect of ethanol extract of P. giganteus (EPG) against lipopolysaccharide (LPS) and combination of LPS and hydrogen peroxide (H2O2)-induced inflammation on RAW 264.7 macrophages was investigated...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27910787/nonvolatile-taste-components-and-antioxidant-properties-of-fruiting-body-and-mycelium-with-high-ergothioneine-content-from-the-culinary-medicinal-golden-oyster-mushroom-pleurotus-citrinopileatus-agaricomycetes
#4
Shin-Yi Lin, Shih-Chang Chien, Sheng-Yang Wang, Jeng-Leun Mau
Pleurotus citrinopileatus mycelium was prepared with high ergothioneine (Hi-Ergo) content and its proximate composition, nonvolatile taste components, and antioxidant properties were studied. The ergothioneine contents of fruiting bodies and Hi-Ergo and regular mycelia were 3.89, 14.57, and 0.37 mg/g dry weight, respectively. Hi-Ergo mycelium contained more dietary fiber, soluble polysaccharides, and ash but less carbohydrates, reducing sugar, fiber, and fat than regular mycelium. However, Hi-Ergo mycelium contained the smallest amounts of total sugars and polyols (47...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910786/bioavailability-and-digestibility-of-nutrients-from-the-dried-oyster-culinary-medicinal-mushroom-pleurotus-ostreatus-agaricomycetes-in-vivo-experiments
#5
Julita Regula, Joanna Suliburska, Marek Siwulski
There is a limited number of publications on the bioavailability and digestibility of nutrients contained in macrofungi. The aim of this study was to assess the bioavailability and digestibility of macronutrients using in vivo experiments on laboratory animals. The experiments were conducted with the commercial oyster mushroom Pleurotus ostreatus. Semisynthetic diets were prepared based on the modified AIN-93M diet and were supplemented with 4% and 8% mushroom powder. Between days 4 and 13, apparent digestibility indexes were determined for all animals using the conventional balance method...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910773/giant-oyster-mushroom-pleurotus-giganteus-agaricomycetes-enhances-adipocyte-differentiation-and-glucose-uptake-via-activation-of-ppar%C3%AE-and-glucose-transporters-1-and-4-in-3t3-l1-cells
#6
Puvaneswari Paravamsivam, Chua Kek Heng, Sri Nurestri Abdul Malek, Vikineswary Sabaratnam, Ravishankar Ram M, Umah Rani Kuppusamy
The edible mushroom Pleurotus giganteus was tested for its effect on adipocyte differentiation and glucose uptake activity in 3T3-L1 cells. The basidiocarps of P. giganteus were soaked in methanol to obtain a crude methanol extract and then fractionated to obtain an ethyl acetate extract. In this study, cell proliferation was measured using an MTT assay, lipid accumulation using an Oil Red O assay, and glucose uptake using a fluorescence glucose uptake assay. Gene expression was measured via real-time polymerase chain reaction analysis with TaqMan primer...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910758/antimicrobial-coumarins-from-the-oyster-culinary-medicinal-mushroom-pleurotus-ostreatus-agaricomycetes-from-kenya
#7
Lilechi D Baraza, Wekesa Neser, Korir Cheruiyot Jackson, Juma B Fredrick, Ochieno Dennis, Kamau R Wairimu, Aggrey Osogo Keya, Matthias Heydenreich
Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ostreatus against selected human pathogenic bacteria and endophytic fungi...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27866467/vitamin-d2-ergosterol-and-vitamin-b2-content-in-commercially-dried-mushrooms-marketed-in-china-and-increased-vitamin-d2-content-following-uv-c-irradiation
#8
Guocheng Huang, Weixi Cai, Baojun Xu
Mushrooms are a great source of vitamin D and vitamin B2; however, the content of these vitamins in dried mushrooms has not fully been investigated. Thus, the objectives of this study were to determine the contents of vitamin D2, ergosterol, and vitamin B2 in commercially dried edible mushrooms in China and to investigate the effect of UV-C irradiation on fresh mushrooms. Among the 35 species of dried mushrooms considered for this study, the average ergosterol content was 1.98 mg/g, while the average vitamin D2 content was 16...
November 21, 2016: International Journal for Vitamin and Nutrition Research. Journal International de Vitaminologie et de Nutrition
https://www.readbyqxmd.com/read/27797216/inhibition-of-oxidative-stress-and-enhancement-of-cellular-activity-by-mushroom-lectins-in-arsenic-induced-carcinogenesis
#9
REVIEW
Tanmoy Rana, Asit Kumar Bera, Subhashree Das, Debasis Bhattacharya, Diganta Pan, Subrata Kumar Das
Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27774430/enhancement-of-the-th1-phenotype-immune-system-by-the-intake-of-oyster-mushroom-tamogitake-extract-in-a-double-blind-placebo-controlled-study
#10
Aiko Tanaka, Mie Nishimura, Yuji Sato, Hiroki Sato, Jun Nishihira
Pleurotus cornucopiae (Oyster mushroom, Tamogitake) has long been eaten as a functional food for enhancement of the immune system, but its effectiveness has not been well confirmed in humans. To this end, we set up a double-blind placebo-controlled human clinical trial to investigate the potential of Oyster mushrooms with respect to the up-regulation of the immune system. The subjects ingested Oyster mushroom extract for 8 weeks. We measured the serum cytokine levels involved in regulation of the immune system, including interferon (IFN)-γ, interleukin (IL)-4, IL-5, IL-10, IL-12, IL-13, and tumor-necrosis factor (TNF)-α...
October 2016: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/27704469/growth-and-yield-performance-of-pleurotus-ostreatus-jacq-fr-kumm-oyster-mushroom-on-different-substrates
#11
Zenebe Girmay, Weldesemayat Gorems, Getachew Birhanu, Solomon Zewdie
Mushroom cultivation is reported as an economically viable bio-technology process for conversion of various lignocellulosic wastes. Given the lack of technology know-how on the cultivation of mushroom, this study was conducted in Wondo Genet College of Forestry and Natural Resource, with the aim to assess the suitability of selected substrates (agricultural and/or forest wastes) for oyster mushroom cultivation. Accordingly, four substrates (cotton seed, paper waste, wheat straw, and sawdust) were tested for their efficacy in oyster mushroom production...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27696289/optimization-of-substrate-preparation-for-oyster-mushroom-pleurotus-ostreatus-cultivation-by-studying-different-raw-materials-and-substrate-preparation-conditions-composting-phases-i-and-ii
#12
Fabrício Rocha Vieira, Meire Cristina Nogueira de Andrade
In recent years, oyster mushroom (Pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in Brazil. Among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. Looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbens grass, brizantha grass and sugarcane straw) and nitrogen supplementation (with or without wheat bran) on oyster mushroom yield and biological efficiency (BE)...
November 2016: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27608429/oral-administration-of-an-aqueous-extract-from-the-oyster-mushroom-pleurotus-ostreatus-enhances-the-immunonutritional-recovery-of-malnourished-mice
#13
Gabriel Llauradó, Humberto J Morris, Yamila Lebeque, Gleymis Venet, Onel Fong, Jane Marcos, Roberto Fontaine, Paul Cos, Rosa C Bermúdez
Mushroom nutriceutical components have lately attracted interest for developing immunonutritional support. However, there is relatively little information pertaining to the use of mushroom preparations for modulating the metabolic and immunological disorders associated to malnutrition. This study was aimed to evaluate the effects of oral administration of an aqueous extract (CW-P) from Pleurotus ostreatus on the recovery of biochemical and immunological functions of malnourished mice. 8-week old female BALB/c mice were starved for 3days and then refed with commercial diet supplemented with or without CW-P (100mg/kg) for 8days...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27592080/pleurotus-citrinopileatus-polysaccharide-induces-activation-of-human-dendritic-cells-through-multiple-pathways
#14
Ken-Ichiro Minato, Lisa C Laan, Akihiro Ohara, Irma van Die
Many edible mushrooms have become attractive as "health foods" and as source materials for immunomodulators. To increase our insight in the immune-modulatory properties of a polysaccharide of the oyster mushroom Pleurotus citrinopileatus, PCPS, we analyzed its effects on the function of human dendritic cells (DCs). We showed that PCPS induces upregulation of the surface maturation markers CD80, CD86 and HLA-DR on DCs, indicating its potential to induce DC maturation. In addition, PCPS stimulates DCs to secrete the pro-inflammatory cytokines TNF, IL-1β, IL-6 and IL-12, as well as the anti-inflammatory cytokine IL-10, and induces enhanced mRNA levels of the chemokines CCL2, CCL3, CCL8, CXCL9, CXCL10, and LTA...
November 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27567720/marker-recycling-via-5-fluoroorotic-acid-and-5-fluorocytosine-counter-selection-in-the-white-rot-agaricomycete-pleurotus-ostreatus
#15
Takehito Nakazawa, Masami Tsuzuki, Toshikazu Irie, Masahiro Sakamoto, Yoichi Honda
Of all of the natural polymers, lignin, an aromatic heteropolymer in plant secondary cell walls, is the most resistant to biological degradation. White-rot fungi are the only known organisms that can depolymerize or modify wood lignin. Investigating the mechanisms underlying lignin biodegradation by white-rot fungi would contribute to the ecofriendly utilization of woody biomass as renewable resources in the future. Efficient gene disruption, which is generally very challenging in the white-rot fungi, was established in Pleurotus ostreatus (the oyster mushroom)...
September 2016: Fungal Biology
https://www.readbyqxmd.com/read/27470278/the-anti-adipogenic-activity-of-a-new-cultivar-pleurotus-eryngii-var-ferulae-beesan-no-2-through-down-regulation-of-ppar-%C3%AE-and-c-ebp-%C3%AE-in-3t3-l1-cells
#16
Min-Jae Kang, Keun Ki Kim, Byoung Yil Son, Soo-Wan Nam, Pyung-Gyun Shin, Gun-Do Kim
Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes. Pleurotus eryngii var. ferulae is one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects...
November 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27294638/iron-bioavailability-from-cereal-products-enriched-with-pleurotus-ostreatus-mushrooms-in-rats-with-induced-anaemia
#17
Julita Reguła, Zbigniew Krejpcio, Halina Staniek
INTRODUCTION AND OBJECTIVE: Oyster mushroom Pleurotus ostreatus is good source of iron. However, there is a limited data concerning bioavailability of iron from oyster mushroom and also cereal products containing this mushroom. The aim of this study was to assess bioavailability of iron from products with an addition of Pleurotus ostreatus in male rats with anaemia. MATERIAL AND METHODS: Investigations were conducted in two stages. In the first stage iron deficiency was developed in rats...
June 2, 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/27279533/protective-effect-of-antioxidant-extracts-from-grey-oyster-mushroom-pleurotus-pulmonarius-agaricomycetes-against-human-low-density-lipoprotein-oxidation-and-aortic-endothelial-cell-damage
#18
Mohamad Hamdi Zainal Abidin, Noorlidah Abdullah, Nurhayati Zainal Abidin
This study evaluated the in vitro antioxidant capacities of extracts from Pleurotus pulmonarius via Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. Extract compositions were determined by phenol-sulfuric acid; Coomassie Plus (Bradford) protein; Spectroquant zinc, copper, and manganese test assays; and liquid chromatography-tandem mass spectrometry (LC/MS/MS) and gas chromatography-mass spectrometry (GC/MS)...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27138199/an-aryl-alcohol-oxidase-of-pleurotus-sapidus-heterologous-expression-characterization-and-application-in-a-2-enzyme-system
#19
Ilya Galperin, Aysha Javeed, Hanno Luig, Günter Lochnit, Martin Rühl
Aryl-alcohol oxidases (AAOs) are enzymes supporting the degradation of lignin by fungal derived class II peroxidases produced by white-rot fungi. AAOs are able to generate H2O2 as a by-product via oxidation of an aryl-alcohol into its correspondent aldehyde. In this study, an AAO was heterologously expressed in a basidiomycete host for the first time. The gene for an AAO of the white-rot fungus Pleurotus sapidus, a close relative to the oyster mushroom Pleurotus ostreatus, was cloned into an expression vector and put under control of the promotor of the glyceraldehyde-3-phosphate dehydrogenase gene 2 (gpdII) of the button mushroom Agaricus bisporus...
September 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27134772/food-derived-hydrophilic-antioxidant-ergothioneine-is-distributed-to-the-brain-and-exerts-antidepressant-effect-in-mice
#20
Noritaka Nakamichi, Keigo Nakayama, Takahiro Ishimoto, Yusuke Masuo, Tomohiko Wakayama, Hirotaka Sekiguchi, Keita Sutoh, Koji Usumi, Shoichi Iseki, Yukio Kato
BACKGROUND: Clinically used antidepressants suffer from various side effects. Therefore, we searched for a safe antidepressant with minimal side effects among food ingredients that are distributed to the brain. Here, we focused on ERGO (ergothioneine), which is a hydrophilic antioxidant and contained at high levels in edible golden oyster mushrooms. ERGO is a typical substrate of carnitine/organic cation transporter OCTN1/SLC22A4, which is expressed in the brain and neuronal stem cells, although little is known about its permeation through the BBB (blood-brain barrier) or its neurological activity...
June 2016: Brain and Behavior
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