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https://www.readbyqxmd.com/read/28194188/primum-non-nocere-first-do-no-harm-and-then-feed-peanut
#1
Kyla Jade Hildebrand, Elissa Michele Abrams, Timothy K Vander Leek, Julia Elizabeth Mainwaring Upton, Douglas P Mack, Linda Kirste, Christine McCusker, Sandeep Kapur
The Addendum Guidelines for the Prevention of Peanut Allergy in the United States-Report of the NIAID-Sponsored Expert Panel were developed to build on previous food allergy guidelines after several key studies demonstrated the benefit of early introduction of allergenic foods. These landmark studies including the Learning Early about Peanut (LEAP), LEAP-On and Enquiring about Tolerance trials created a paradigm shift in food allergy prevention. The "take home" messages of this guideline include that peanut should be introduced early in the first year of life, and for the majority of infants, peanut can be introduced at home...
2017: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/28186805/simultaneous-bistability-of-a-qubit-and-resonator-in-circuit-quantum-electrodynamics
#2
Th K Mavrogordatos, G Tancredi, M Elliott, M J Peterer, A Patterson, J Rahamim, P J Leek, E Ginossar, M H Szymańska
We explore the joint activated dynamics exhibited by two quantum degrees of freedom: a cavity mode oscillator which is strongly coupled to a superconducting qubit in the strongly coherently driven dispersive regime. Dynamical simulations and complementary measurements show a range of parameters where both the cavity and the qubit exhibit sudden simultaneous switching between two metastable states. This manifests in ensemble averaged amplitudes of both the cavity and qubit exhibiting a partial coherent cancellation...
January 27, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28172893/p297-fecal-calprotectin-accurately-predicts-symptomatic-relapse-in-children-and-adolescents-with-inflammatory-bowel-disease-in-clinical-remission
#3
K Diederen, D Hoekman, A Leek, V Wolters, M Benninga, T Hummel, T de Meij, B Koot, M Tabbers, M Benninga, A Kindermann
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28171598/widespread-splicing-of-repetitive-element-loci-into-coding-regions-of-gene-transcripts
#4
Miranda M Darby, Jeffrey T Leek, Ben Langmead, Robert H Yolken, Sarven Sabunciyan
No abstract text is available yet for this article.
November 15, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/28144174/an-early-record-of-meloidogyne-fallax-from-ireland
#5
Olivera Topalović, John F Moore, Toon Janssen, Wim Bert, Gerrit Karssen
Root-knot nematodes, Meloidogyne spp., cause huge economic losses worldwide. Currently, three Meloidogyne spp. are present on the quarantine A2 list of EPPO, Meloidogyne chitwoodi, Meloidogyne fallax and Meloidogyne enterolobii. As a quarantine organism, Meloidogyne fallax has been detected in England and Northern Ireland on sport turf in 2011, and in England on leek in 2013. However, its presence in Ireland has probably been overlooked since 1965, when Mr. John F. Moore and Dr. Mary T. Franklin had detected a new Meloidogyne species for that time...
2017: ZooKeys
https://www.readbyqxmd.com/read/28138990/raised-faecal-calprotectin-is-associated-with-subsequent-symptomatic-relapse-in-children-and-adolescents-with-inflammatory-bowel-disease-in-clinical-remission
#6
K Diederen, D R Hoekman, A Leek, V M Wolters, T Z Hummel, T G de Meij, B G P Koot, M M Tabbers, M A Benninga, A Kindermann
BACKGROUND: Reliable data on inflammatory biomarkers for predicting relapse of paediatric inflammatory bowel disease (IBD) are lacking. AIM: To investigate the predictive value of faecal calprotectin (FC) and CRP for symptomatic relapse in pediatric IBD in clinical remission. METHODS: In this cross-sectional cohort study, patients <18 years with Crohn's disease or ulcerative colitis in clinical remission ≥3 months were included. At baseline, clinical and biochemical disease activity were assessed using the abbreviated-Pediatric Crohn's Disease Activity Index or Pediatric Ulcerative Colitis Activity Index, and FC and CRP respectively...
January 31, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28134423/tobacco-chewing-and-risk-of-gastric-cancer-a-case-control-study-in-yemen
#7
F A Al-Qadasi, S A Shah, H F Ghazi
This study aimed to assess the risk factors for gastric cancer in Yemen. A hospital-based case-control study of 70 cases and 140 controls was carried out in Sana'a city between May and October 2014. A structured questionnaire was used to collect information through direct interview. Living in rural areas, tobacco chewing and drinking untreated water were significant risk factors for gastric cancer. Frequent consumption of chicken, cheese, milk, starchy vegetables, cucumber, carrots, leeks, sweet pepper, fruit drinks, legumes and olive oil were associated significantly with decreased risk of gastric cancer...
January 23, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/28107356/integrity-of-induced-pluripotent-stem-cell-ipsc-derived-megakaryocytes-as-assessed-by-genetic-and-transcriptomic-analysis
#8
Kai Kammers, Margaret A Taub, Ingo Ruczinski, Joshua Martin, Lisa R Yanek, Alyssa Frazee, Yongxing Gao, Dixie Hoyle, Nauder Faraday, Diane M Becker, Linzhao Cheng, Zack Z Wang, Jeff T Leek, Lewis C Becker, Rasika A Mathias
Previously, we have described our feeder-free, xeno-free approach to generate megakaryocytes (MKs) in culture from human induced pluripotent stem cells (iPSCs). Here, we focus specifically on the integrity of these MKs using: (1) genotype discordance between parent cell DNA to iPSC cell DNA and onward to the differentiated MK DNA; (2) genomic structural integrity using copy number variation (CNV); and (3) transcriptomic signatures of the derived MK lines compared to the iPSC lines. We detected a very low rate of genotype discordance; estimates were 0...
2017: PloS One
https://www.readbyqxmd.com/read/28106796/preparative-scale-resolution-of-enantiomers-enables-accelerated-drug-discovery-and-development
#9
Hanna Leek, Shalini Andersson
The provision of pure enantiomers is of increasing importance not only for the pharmaceutical industry but also for agro-chemistry and biotechnology. In drug discovery and development, the enantiomers of a chiral drug depict unique chemical and pharmacological behaviors in a chiral environment, such as the human body, in which the stereochemistry of the chiral drugs determines their pharmacokinetic, pharmacodynamic and toxicological properties. We present a number of challenging case studies of up-to-kilogram separations of racemic or enriched isomer mixtures using preparative liquid chromatography and super critical fluid chromatography to generate individual enantiomers that have enabled the development of new candidate drugs within AstraZeneca...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28038678/human-splicing-diversity-and-the-extent-of-unannotated-splice-junctions-across-human-rna-seq-samples-on-the-sequence-read-archive
#10
Abhinav Nellore, Andrew E Jaffe, Jean-Philippe Fortin, José Alquicira-Hernández, Leonardo Collado-Torres, Siruo Wang, Robert A Phillips Iii, Nishika Karbhari, Kasper D Hansen, Ben Langmead, Jeffrey T Leek
BACKGROUND: Gene annotations, such as those in GENCODE, are derived primarily from alignments of spliced cDNA sequences and protein sequences. The impact of RNA-seq data on annotation has been confined to major projects like ENCODE and Illumina Body Map 2.0. RESULTS: We aligned 21,504 Illumina-sequenced human RNA-seq samples from the Sequence Read Archive (SRA) to the human genome and compared detected exon-exon junctions with junctions in several recent gene annotations...
December 30, 2016: Genome Biology
https://www.readbyqxmd.com/read/27999077/analysis-of-dithiocarbamate-fungicides-in-vegetable-matrices-using-hplc-uv-followed-by-atomic-absorption-spectrometry
#11
Josephine Al-Alam, Laura Bom, Asma Chbani, Ziad Fajloun, Maurice Millet
A simple method combining ion-pair methylation, high-performance liquid chromatography (HPLC) analysis with detection at 272 nm and atomic absorption spectrometry was developed in order to determine 10 dithiocarbamate fungicides (Dazomet, Metam-sodium, Ferbam, Ziram, Zineb, Maneb, Mancozeb, Metiram, Nabam and Propineb) and distinguish ethylenbisdithiocarbamates (EBDTCs) Zineb, Maneb and Mancozeb in diverse matrices. This method associates reverse phase analysis by HPLC analysis with detection at 272 nm, with atomic absorption spectrometry in order to distinguish, with the same extraction protocol, Maneb, Mancozeb and Zineb...
December 19, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27998093/cucurbit-7-uril-tetramethylrhodamine-conjugate-for-direct-sensing-and-cellular-imaging
#12
Andrew T Bockus, Lauren C Smith, Amy G Grice, Omar A Ali, Carolyn C Young, William Mobley, Ashley Leek, James L Roberts, Brittany Vinciguerra, Lyle Isaacs, Adam R Urbach
This paper describes the design and synthesis of a conjugate (Q7R) comprising the synthetic host cucurbit[7]uril (Q7) linked to the fluorescent dye tetramethylrhodamine (TMR), and the characterization of its optical and guest-binding properties as well as its cellular uptake. Q7R was synthesized in two steps from monofunctionalized azidobutyl-Q7 and NHS-activated TMR. The fluorescence of Q7R is quenched upon guest binding, and this observable was used to determine equilibrium dissociation constant (Kd) values...
December 21, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27979194/a-strategy-for-the-evaluation-of-an-analytical-approach-for-selected-pesticide-residues-in-complex-agricultural-product-matrices-a-case-study-of-leek
#13
Yanyan Xu, Ninghui Song, Qin Zhang, Jining Liu, Guosong Chen, Lili Shi, Jikui Wang
In order to choose a sensitive, selective, simple, rapid, cost-effective and reliable analytical method for a given complex sample, a strategy was proposed for the evaluation of an analytical approach to determining multi-pesticide residue in complex agricultural product matrices, using leek as an example. In this work, the matrix effect of most of pesticides were in the range of -50% to -90%. A simple thin layer chromatography (TLC) was used to evaluate the effect of cleanup. The results agree well with that of matrix effect described above...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27979090/a-study-on-biomimetic-immunoassay-capillary-electrophoresis-method-based-on-molecularly-imprinted-polymer-for-determination-of-trace-trichlorfon-residue-in-vegetables
#14
Jie Li, Juxiu Lu, Xuguang Qiao, Zhixiang Xu
Pesticide residue in vegetables is a serious problem that has adverse effects on human health. In our study, we designed and synthesized a molecularly imprinted polymer that can selectively recognize trichlorfon. Using the polymer material as biomimetic antibody, we developed a biomimetic immunoassay-capillary electrophoresis method with improved sensitivity for the detection of trichlorfon. We evaluated the competitive reactions between HRP labeled trichlorfon hapten and free trichlorfon with the biomimetic antibody...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27955886/generation-characterization-and-reuse-of-solid-wastes-from-a-biodiesel-production-plant
#15
Fernando Jorge Santos Oliveira, Daniele Dos Santos Santana, Simone Soraya Brito Costa, Lenise Diniz Oliveira, Vitor Silva Liduino, Eliana Flávia Camporese Servulo
The aim of this study was to identify and characterize industrial solid wastes generated by a biodiesel production plant in Brazil, as well as to present strategies for the management of these materials. This plant produces every year around 100,000tons of biodiesel from vegetable oils and animal fats. The methodology of the study included technical visits, interviews with the operational and environmental management staff as well as analysis of documents, reports and computerized data systems. An approach to reduce the generation of hazardous waste was investigated...
December 7, 2016: Waste Management
https://www.readbyqxmd.com/read/27914370/concurrent-measures-of-contralateral-suppression-of-transient-evoked-otoacoustic-emissions-and-of-auditory-steady-state-responses
#16
Ian B Mertes, Marjorie R Leek
Contralateral suppression of otoacoustic emissions (OAEs) is frequently used to assess the medial olivocochlear (MOC) efferent system, and may have clinical utility. However, OAEs are weak or absent in hearing-impaired ears, so little is known about MOC function in the presence of hearing loss. A potential alternative measure is contralateral suppression of the auditory steady-state response (ASSR) because ASSRs are measurable in many hearing-impaired ears. This study compared contralateral suppression of both transient-evoked otoacoustic emissions (TEOAEs) and ASSRs in a group of ten primarily older adults with either normal hearing or mild sensorineural hearing loss...
September 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27871350/early-description-of-diet-induced-blistering-skin-diseases-in-medieval-persia-avicenna-s-point-of-view
#17
Fatemeh Atarzadeh, Babak Daneshfard, Ladan Dastgheib, Amir-Mohammad Jaladat, Gholamreza Amin
Pemphigus is an autoimmune blistering skin disease that is strongly associated with different environmental factors. Among these, nutritional factors are considered to trigger pemphigus; however, their role may be underestimated. Investigated more recently in conventional medicine, this causative bond between dietary factors and blistering skin diseases was mentioned by Persian scholars such as Avicenna a thousand years ago. Avicenna, a well-known Persian physician and philosopher, who could be considered a pioneer in dermatology, discussed skin diseases in a chapter in The Canon of Medicine...
2016: Skinmed
https://www.readbyqxmd.com/read/27836984/recurrent-innovation-at-genes-required-for-telomere-integrity-in-drosophila
#18
Yuh Chwen G Lee, Courtney Leek, Mia T Levine
Telomeres are nucleoprotein complexes at the ends of linear chromosomes. These specialized structures ensure genome integrity and faithful chromosome inheritance. Recurrent addition of repetitive, telomere-specific DNA elements to chromosome ends combats end-attrition, while specialized telomere-associated proteins protect naked, double-stranded chromosome ends from promiscuous repair into end-to-end fusions. Although telomere length homeostasis and end-protection are ubiquitous across eukaryotes, there is sporadic but building evidence that the molecular machinery supporting these essential processes evolves rapidly...
November 11, 2016: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/27765204/inorganic-selenium-speciation-analysis-in-allium-and-brassica-vegetables-by-ionic-liquid-assisted-liquid-liquid-microextraction-with-multivariate-optimization
#19
Alexander Castro Grijalba, Estefanía M Martinis, Rodolfo G Wuilloud
A highly sensitive vortex assisted liquid-liquid microextraction (VA-LLME) method was developed for inorganic Se [Se(IV) and Se(VI)] speciation analysis in Allium and Brassica vegetables. Trihexyl(tetradecyl)phosphonium decanoate phosphonium ionic liquid (IL) was applied for the extraction of Se(IV)-ammonium pyrrolidine dithiocarbamate (APDC) complex followed by Se determination with electrothermal atomic absorption spectrometry. A complete optimization of the graphite furnace temperature program was developed for accurate determination of Se in the IL-enriched extracts and multivariate statistical optimization was performed to define the conditions for the highest extraction efficiency...
March 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27728958/description-of-the-ambulance-services-participating-in-the-aus-roc-australian-and-new-zealand-out-of-hospital-cardiac-arrest-epistry
#20
Ben Beck, Janet E Bray, Karen Smith, Tony Walker, Hugh Grantham, Cindy Hein, Melanie Thorrowgood, Anthony Smith, Madoka Inoue, Tony Smith, Bridget Dicker, Andy Swain, Emma Bosley, Katherine Pemberton, Michael McKay, Malcolm Johnston-Leek, Peter Cameron, Gavin D Perkins, Judith Finn
OBJECTIVE: The present study aimed to describe and examine similarities and differences in the current service provision and resuscitation protocols of the ambulance services participating in the Aus-ROC Australian and New Zealand out-of-hospital cardiac arrest (OHCA) Epistry. Understanding these similarities and differences is important in identifying ambulance service factors that might explain regional variation in survival of OHCA in the Aus-ROC Epistry. METHODS: A structured questionnaire was completed by each of the ambulance services participating in the Aus-ROC Epistry...
December 2016: Emergency Medicine Australasia: EMA
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