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Anastasija Zaiko, Jose L Martinez, Alba Ardura, Laura Clusa, Yaisel J Borrell, Aurelija Samuiloviene, Agustín Roca, Eva Garcia-Vazquez
Detecting the presence of potential invasive species in ballast water is a priority for preventing their spread into new environments. Next generation sequencing technologies are being increasingly used for exploring and assessing biodiversity from environmental samples. Here we apply high throughput sequencing from DNA extracted from ballast water (BW) samples employing two different platforms, Ion Torrent and 454, and compare the putative species catalogues from the resulting Operational Taxonomic Units (OTU)...
December 2015: Marine Environmental Research
A T Nurden, P Nurden
The gene variants responsible for the primary genotype of many platelet disorders have now been identified. Next-generation sequencing technology (NGST), mainly exome sequencing, has highlighted genes responsible for defects in platelet secretion (NBEAL2, gray platelet syndrome), procoagulant activity (STIM1, Stormorken syndrome), and activation pathways (RASGRP2, CalDAG-GEFI deficiency and integrin dysfunction; PRKACG, cyclic adenosine monophosphate-dependent protein kinase deficiency). Often disorders of platelet function are associated with a modified platelet production with changes in platelet number and size and can accompany malfunction of other organs or tissues...
June 2015: Journal of Thrombosis and Haemostasis: JTH
Rhea Y Y Tan, Hugh S Markus
Most stroke is multifactorial with multiple polygenic risk factors each conferring small increases in risk interacting with environmental risk factors, but it can also arise from mutations in a single gene. This review covers single-gene disorders which lead to stroke as a major phenotype, with a focus on those which cause cerebral small vessel disease (SVD), an area where there has been significant recent progress with findings that may inform us about the pathogenesis of SVD more broadly. We also discuss the impact that next generation sequencing technology (NGST) is likely to have on clinical practice in this area...
December 2015: Journal of Neurology
Choongheon Lee, Julie A Honaker
The gaze stabilization test (GST) is used not only in examining gaze stability required by daily life activities, but also in identifying unilateral or bilateral vestibular deficits. However, a computerized GST (CGST) is an expensive assessment, which cannot be commonly used in most clinics. Validation of low-cost and low-technical clinical tests is required to decrease health care costs. The purpose of this study was to measure the accuracy of a new GST (NGST) in a cohort of healthy young adults with no history of vestibular or balance disorders, as well as the test-retest reliability when re-assessed 5-7 days...
January 1, 2013: Journal of Vestibular Research: Equilibrium & Orientation
Graham M Hughes, Li Gang, William J Murphy, Desmond G Higgins, Emma C Teeling
The advent of Next Generation Sequencing Technology (NGST) has revolutionized molecular biology research, allowing for rapid gene/genome sequencing from a multitude of diverse species. As high throughput sequencing becomes more accessible, more efficient workflows must be developed to deal with the amounts of data produced and better assemble the genomes of de novo lineages. We combine traditional laboratory methods with Illumina NGST to amplify and sequence the largest mammalian multigene family, the Olfactory Receptor gene family, for species with and without a reference genome...
May 2013: Molecular Ecology Resources
Agnes Dettai, Cyril Gallut, Sophie Brouillet, Joel Pothier, Guillaume Lecointre, Régis Debruyne
BACKGROUND: Researchers sorely need markers and approaches for biodiversity exploration (both specimen linked and metagenomics) using the full potential of next generation sequencing technologies (NGST). Currently, most studies rely on expensive multiple tagging, PCR primer universality and/or the use of few markers, sometimes with insufficient variability. METHODOLOGY/PRINCIPAL FINDINGS: We propose a novel approach for the isolation and sequencing of a universal, useful and popular marker across distant, non-model metazoans: the complete mitochondrial genome...
2012: PloS One
Antonis Rokas, John G Gibbons, Xiaofan Zhou, Anne Beauvais, Jean-Paul Latgé
The deep sequencing of an mRNA population, RNA-seq, is a very successful application of next-generation sequencing technologies (NGSTs). RNA-seq takes advantage of two key NGST features: (1) samples can be mixtures of different DNA pieces, and (2) sequencing provides both qualitative and quantitative information about each DNA piece analyzed. We recently used RNA-seq to study the transcriptome of Aspergillus fumigatus, a deadly human fungal pathogen. Analysis of the RNA-seq data indicates that there are likely tens of unannotated and hundreds of novel genes in the A...
December 2012: Annals of the New York Academy of Sciences
Ching-Ching Yu, Yu-Ying Kuo, Chien-Fu Liang, Wei-Ting Chien, Huan-Ting Wu, Tsung-Che Chang, Fan-Dan Jan, Chun-Cheng Lin
Magnetic nanoparticles (MNPs) are attractive materials that serve as a support for enzyme immobilization and facilitate separations by applying an external magnetic field; this could facilitate the recycling of enzymes and broaden their applications in organic synthesis. Herein, we report the methods for the immobilization of water-soluble and membrane-bound enzymes, and the activity difference between free and immobilized enzymes is discussed. Sialyltransferase (PmST1, from Pasteurella multocida ) and cytidine monophosphate (CMP)-sialic acid synthetase (CSS, from Neisseria meningitides ) were chosen as water-soluble enzymes and expressed using an intein expression system...
April 18, 2012: Bioconjugate Chemistry
Zsuzsanna Mihály, Balázs Gyorffy
In the past ten years the development of next generation sequencing technologies brought a new era in the field of quick and efficient DNA sequencing. In our study we give an overview of the methodological achievements from Sanger's chain-termination sequencing in 1975 to those allowing real-time DNA sequencing today. Sequencing methods that utilize clonal amplicons for parallel multistrand sequencing comprise the basics of currently available next generation sequencing techniques. Nowadays next generation sequencing is mainly used for basic research in functional genomics, providing quintessential information in the meta-analyses of data from signal transduction pathways, onthologies, proteomics and metabolomics...
January 9, 2011: Orvosi Hetilap
A Hellemans
NASA scientists and engineers working on the Next Generation Space Telescope (NGST) got a boost from across the Atlantic last week. On 15 September in Paris, the European Space Agency's top science advisory committee recommended that the agency become a major partner in the project. The recommendation puts NGST--along with a handful of other missions the committee also endorsed last week--one short step away from officially becoming part of Europe's space program.
September 22, 2000: Science
Jay Shankar, Bhanu P Singh, Shailendra N Gaur, Naveen Arora
Recombinant proteins are used for vaccines, therapy and diagnosis of many diseases. Biological activity of these may differ from native counterpart and needs investigation. The present study aimed to compare recombinant (r) and native (n) glutathione-S-transferase (GST) from Alternaria alternata. Glutathione-S-transferase sequence showed an ORF of 696bp encoding 26-kDa protein with N-terminus conserved domain. Secondary structure of both forms was comparable with melting temperature of 57 and 59 degrees C, respectively...
May 2006: Molecular Immunology
Andrew Lawler
No abstract text is available yet for this article.
February 22, 2002: Science
M Hadders-Algra
The Neuronal Group Selection Theory (NGST) could offer new insights into the mechanisms directing motor disorders, such as cerebral palsy and developmental coordination disorder. According to NGST, normal motor development is characterized by two phases of variability. Variation is not at random but determined by criteria set by genetic information. Development starts with the phase of primary variability, during which variation in motor behavior is not geared to external conditions. At function-specific ages secondary variability starts, during which motor performance can be adapted to specific situations...
2001: Neural Plasticity
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