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Langendorff

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https://www.readbyqxmd.com/read/29677462/ondansetron-blocks-wildtype-and-p-f503l-variant-small-conductance-calcium-activated-potassium-channels
#1
Jum-Suk Ko, Shuai Guo, Jonathan Hassel, Patricia Celestino-Soper, Ty C Lynnes, James E Tisdale, James J Zheng, Stanley E Taylor, Tatiana Foroud, Michael D Murray, Richard J Kovacs, Xiaochun Li, Shien-Fong Lin, Zhenhui Chen, Matteo Vatta, Peng-Sheng Chen, Michael Rubart
The apamin-sensitive, small conductance, calcium-activated potassium (SK) current ( IKAS ) is encoded by KCNN genes. IKAS importantly contributes to cardiac repolarization in conditions associated with reduced repolarization reserve. To test the hypothesis that IKAS inhibition contributes to drug-induced long QT (diLQT) syndrome, we (1) screened for KCNN variants among patients with diLQT, (2) determined the properties of heterologously expressed wildtype and variant KCNN channels, and (3) determined if the 5-HT3 receptor antagonist ondansetron blocks IKAS ...
April 20, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29671257/glycogen-synthase-kinase-3%C3%AE-opens-mitochondrial-permeability-transition-pore-through-mitochondrial-hexokinase-ii-dissociation
#2
Takamitsu Tanaka, Masao Saotome, Hideki Katoh, Terumori Satoh, Prottoy Hasan, Hayato Ohtani, Hiroshi Satoh, Hideharu Hayashi, Yuichiro Maekawa
Accumulating evidence has revealed pivotal roles of glycogen synthase kinase-3β (GSK3β) inactivation on cardiac protection. Because the precise mechanisms of cardiac protection against ischemia/reperfusion (I/R) injury by GSK3β-inactivation remain elusive, we investigated the relationship between GSK3β-mediated mitochondrial hexokinase II (mitoHK-II; a downstream target of GSK3β) dissociation and mitochondrial permeability transition pore (mPTP) opening. In Langendorff-perfused hearts, GSK3β inactivation by SB216763 improved the left ventricular-developed pressure and retained mitoHK-II binding after I/R...
April 18, 2018: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/29670839/effects-of-postconditioning-with-fructose-on-arrhythmias-and-the-size-of-infarct-caused-by-global-ischemia-and-reperfusion-in-isolated-rat-heart
#3
Jila Haghi, Tahereh Eteraf-Oskouei, Moslem Najafi
Purpose: In the present study, postconditioning effect of fructose against ischemia/reperfusion (I/R)-induced arrhythmias and infarct size were investigated in isolated rat heart. Methods: The isolated hearts were divided into 7 groups, mounted on a Langendorff apparatus at constant pressure then subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, normal Krebs-Henseleit (K/H) solution was perfused into the hearts throughout the experiment. In two separate sets of experiments, the treatment groups received 12, 24 and 48 mM of fructose with/without normal glucose in K/H solution for 20 min at the beginning of reperfusion...
March 2018: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29670333/upregulation-of-vascular-endothelial-growth-factor-receptor-1-contributes-to-sevoflurane-preconditioning-mediated-cardioprotection
#4
Bin Qian, Yang Yang, Yusheng Yao, Yanling Liao, Ying Lin
Purpose: Sevoflurane preconditioning (SPC) can provide myocardial protective effects similar to ischemic preconditioning. However, the exact mechanism of SPC remains unclear. Previous studies indicate that vascular endothelial growth factor receptor 1 (VEGFR-1) is involved in ischemic preconditioning-mediated cardioprotection. This study was designed to determine the significance of VEGFR-1 signaling in SPC-mediated cardioprotection. Materials and methods: Myocardial ischemia-reperfusion (I/R) rat model was established using the Langendorff isolated heart perfusion apparatus...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29656359/myocardial-infarct-size-reduction-provided-by-local-and-remote-ischaemic-preconditioning-references-values-from-the-hatter-cardiovascular-institute
#5
Xavier Rossello, Zhenhe He, Derek M Yellon
PURPOSE: To accurately estimate the effect size of both local or classic ischaemic preconditioning (IPC) and remote ischaemic preconditioning (RIPC) using a pooling data set of 91 animals. METHODS: We combined all the available mouse data collected from our Institute over the last 3 years regarding (i) local IPC (4 cycles of 5 min of global ischaemia/reperfusion injury, IRI, followed by 35-min ischaemia and 2-h reperfusion) in the Langendorff-isolated perfused mouse heart model and (ii) RIPC (3 cycles of 5 min of limb occlusion followed by 40-min ischaemia and 2-h reperfusion) in the in vivo mouse model...
April 14, 2018: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/29626474/canstatin-modulates-l-type-calcium-channel-activity-in-rat-ventricular-cardiomyocytes
#6
Keisuke Imoto, Masaki Hirakawa, Muneyoshi Okada, Hideyuki Yamawaki
Excessive increase of cytosolic Ca2+ through the activation of L-type Ca2+ channels (LTCCs) via β adrenergic receptor induces apoptosis of cardiomyocytes. Canstatin, a cleaved fragment of collagen type IV α2 chain, is abundantly expressed in normal heart tissue. We previously reported that canstatin inhibits β adrenergic receptor-stimulated apoptosis in cardiomyoblasts. Here, we tested the hyposeis that canstatin regulates LTCCs activity in ventricular cardiomyocytes. Collagen type IV α2 chain (COL4A2) small interfering (si) RNA (for canstatin suppression) or control siRNA was injected via jugular vein in Wistar rats...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29620570/free-fatty-acid-receptor-g-protein-coupled-receptor-40-mediates-lipid-emulsion-induced-cardioprotection
#7
Soban Umar, Jingyuan Li, Kyle Hannabass, Mylene Vaillancourt, Christine M Cunningham, Shayan Moazeni, Aman Mahajan, Mansoureh Eghbali
BACKGROUND: We have previously shown that intralipid (lipid emulsion) protects the heart against ischemia/reperfusion injury and bupivacaine-induced cardiotoxicity. However, the precise underlying mechanisms are not fully understood. Here we explored the hypothesis that free fatty acid receptor-1 or G-protein-coupled receptor 40 is expressed in the heart and that cardioprotective effects of lipid emulsion are mediated through G-protein-coupled receptor 40 in two animal models of ischemia/reperfusion injury and bupivacaine-induced cardiotoxicity...
April 4, 2018: Anesthesiology
https://www.readbyqxmd.com/read/29615543/intermittent-hypoxia-induces-beneficial-cardiovascular-remodeling-in-left-ventricular-function-of-type-1-diabetic-rat
#8
Fırat Akat, Hakan Fıçıcılar, Ayşegül Durak, Erkan Tuncay, Ali Doğan Dursun, Ferda Topal Çelikkan, Bizden Sabuncuoğlu, Belma Turan, Metin Baştuğ
OBJECTIVE: Depressed mechanical activity is a marked complication in diabetics. Hypoxia has properties for novel diagnostic and therapeutic strategies, while intermittent hypoxia (IH) provides early functional and histologic remodeling, including some cardio benefits in early hemodynamic alterations with histologic remodeling and delayed changes in peripheral vasoreactivity. Therefore, we aimed to examine whether IH application presents a cardioprotective effect, via stabilization of hypoxia-inducible factor (HIF) in streptozotocin (STZ)-induced diabetic rat heart...
April 2018: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/29604187/investigating-the-possible-mechanisms-involved-in-adenosine-preconditioning-induced-cardioprotection-in-rats
#9
Lovedeep Singh, Jasleen Kaur Virdi, L N Maslov, Nirmal Singh, Amteshwar Singh Jaggi
BACKGROUND: Adenosine is a breakdown product of adenosine triphosphate and plays an important role in pharmacological preconditioning. The cardioprotective effects of adenosine preconditioning are well established. However, the possible mechanisms need to be explored. AIM: The present study was aimed to investigate the possible mechanisms involved in adenosine preconditioning-induced cardioprotection in rats. METHODS: Rat heart was isolated and perfused on Langendorff apparatus...
March 31, 2018: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/29582211/the-caspase-1-inhibitor-vx-765-protects-the-isolated-rat-heart-via-the-risk-pathway
#10
Helison Do Carmo, Sapna Arjun, Orlando Petrucci, Derek M Yellon, Sean M Davidson
PURPOSE: Protecting the heart from ischaemia-reperfusion (IR) injury is a major goal in patients presenting with an acute myocardial infarction. Pyroptosis is a novel form of cell death in which caspase 1 is activated and cleaves interleukin 1β. VX-785 is a highly selective, prodrug caspase 1 inhibitor that is also clinically available. It has been shown to be protective against acute IR in vivo rat model, and therefore might be a promising possibility for future cardioprotective therapy...
March 26, 2018: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/29581158/chymase-released-from-hypoxia-activated-cardiac-mast-cells-cleaves-human-apolipoproteina-i-at-tyr192-and-compromises-its-cardioprotective-activity
#11
Ilona Kareinen, Marc Baumann, Su Duy Nguyen, Katariina Maaninka, Andrey Anisimov, Minoru Tozuka, Matti Jauhiainen, Miriam Lee-Rueckert, Petri T Kovanen
Apolipoprotein A-I (apoA-I), the main structural and functional protein of HDL particles, is cardioprotective, but also highly sensitive to proteolytic cleavage. Here we investigated the effect of cardiac mast cell activation and ensuing chymase secretion on apoA-I degradation using isolated rat hearts in the Langendorff perfusion system. Cardiac mast cells were activated by injection of compound 48/80 into the coronary circulation or by low-flow myocardial ischemia, after which lipid-free apoA-I was injected, and collected in the coronary effluent for cleavage analysis...
March 26, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29579593/amp-activated-protein-kinase-protects-against-necroptosis-via-regulation-of-keap1-pgam5-complex
#12
Yi-Shu Wang, Peng Yu, Yong Wang, Jing Zhang, Wei Hang, Zhi-Xian Yin, Gang Liu, Jianfeng Chen, Kaitlin D Werle, Cheng-Shi Quan, Hang Gao, Qinghua Zeng, Rutao Cui, Jiyong Liang, Qiang Ding, Yu-Lin Li, Zhi-Xiang Xu
BACKGROUND: The AMP-activated protein kinase (AMPK) plays critical roles in growth regulation and metabolism reprogramming. AMPK activation protects cells against apoptosis from injury in different cell and animal models. However, its function in necroptosis remains largely unclear. METHODS AND RESULTS: In the current study, we demonstrated that AMPK was activated upon necroptosis induction and protected mouse embryonic fibroblasts (MEFs) and cardiomyocytes from N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and reactive oxygen species (ROS) induced necroptosis...
May 15, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29575758/a-selenoprotein-t-derived-peptide-protects-the-heart-against-ischemia-reperfusion-injury-through-inhibition-of-apoptosis-and-oxidative-stress
#13
Carmine Rocca, Loubna Boukhzar, Maria Concetta Granieri, Ifat Alsharif, Rosa Mazza, Benjamin Lefranc, Bruno Tota, Jérôme Leprince, Maria Carmela Cerra, Youssef Anouar, Tommaso Angelone
AIM: Selenoprotein T (SelT or SELENOT) is a novel thioredoxin-like enzyme whose genetic ablation in mice results in early embryonic lethality. SelT exerts an essential cytoprotective action during development and after injury through its redox-active catalytic site. The present study aims to determine the expression and regulation of SelT in the mammalian heart in normal and pathological conditions, and to evaluate the cardioprotective effect of a SelT-derived peptide, SelT43-52(PSELT) encompassing the redox motif which is key to its function, against ischemia/reperfusion(I/R) injury...
March 25, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29573617/anti-arrhythmogenic-and-anti-inflammatory-effects-of-troxerutin-in-ischemia-reperfusion-injury-of-diabetic-myocardium
#14
Moslem Najafi, Elham Noroozi, Aniseh Javadi, Reza Badalzadeh
INTRODUCTION: Medicinal plants are increasingly used in the treatment of cardiovascular diseases due to their multifaceted properties. This study was designed to investigate anti-arrhythmic and anti-inflammatory potentials of the natural bioflavonoid, troxerutin (TXR) in myocardial ischemia/reperfusion (I/R) injury in diabetic rats. METHODS: Male Wistar rats were randomly divided into 4 groups (control, control + TXR [150 mg/kg, daily], diabetic, and diabetic + TXR)...
March 21, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29571157/antimalarial-agents-against-both-sexual-and-asexual-parasites-stages-structure-activity-relationships-and-biological-studies-of-the-malaria-box-compound-1-5-4-bromo-2-chlorophenyl-furan-2-yl-n-piperidin-4-yl-methyl-methanamine-mmv019918-and-analogues
#15
Alessandra Vallone, Sarah D'Alessandro, Simone Brogi, Margherita Brindisi, Giulia Chemi, Gloria Alfano, Stefania Lamponi, Soon Goo Lee, Joseph M Jez, Karin J M Koolen, Koen J Dechering, Simona Saponara, Fabio Fusi, Beatrice Gorelli, Donatella Taramelli, Silvia Parapini, Reto Caldelari, Giuseppe Campiani, Sandra Gemma, Stefania Butini
Therapies addressing multiple stages of Plasmodium falciparum life cycle are highly desirable for implementing malaria elimination strategies. MMV019918 (1, 1-[5-(4-bromo-2-chlorophenyl)furan-2-yl]-N-[(piperidin-4-yl)methyl]methanamine) was selected from the MMV Malaria Box for its dual activity against both asexual stages and gametocytes. In-depth structure-activity relationship studies and cytotoxicity evaluation led to the selection of 25 for further biological investigation. The potential transmission blocking activity of 25 versus P...
March 10, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29569577/effects-of-volatile-anesthetic-preconditioning-on-expression-of-nfkb-regulated-genes-in-aged-rat-myocardium
#16
Zhong Cai-Yun, Qiu Hong, Chen Jun, Liu Hong
Volatile anesthetic preconditioning has been shown to be a potent way to provide myocardium protection against ischemia/reperfusion (I/R) injury; however, this cardioprotection is lost in senescent animal models and elderly patients. NFkB-regulated genes have been linked to myocardial I/R injury and anesthetic preconditioning. Here, we investigated NFkB activation related to anesthetic preconditioning in aging rat myocardium. Isolated, Langendorff perfused rat hearts from Fischer 344 male rats, 24 months old, were randomly assigned to one of the three groups...
November 1, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/29559303/remifentanil-preconditioning-confers-cardioprotection-via-c-jun-nh-2-terminal-kinases-and-extracellular-signal-regulated-kinases-pathways-in-ex-vivo-failing-rat-heart
#17
Shi-Yun Jin, Jun Huang, Hai-Juan Zhu, Hao Wu, Shi-Jin Xu, Michael G Irwin, Shu-Fang He, Ye Zhang
Remifentanil preconditioning (RPC) exerts protection in normal hearts, but has not been investigated in heart failure. The aim of the present study was to evaluate the effect of RPC in a chronic failing rat heart model and the mechanisms involving mitogen-activated protein kinases (MAPK) and Bcl-2 protein family. The doxorubicin induced failing rat hearts were subjected to 30min ischemia / 120min reperfusion (IR) with or without RPC by using Langendorff apparatus. RPC was induced by three cycles of 5min remifentanil / 5min drug-free perfusion before IR, with three different concentrations: 25, 50 and 100μg/l...
March 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29554082/postnatal-developmental-changes-in-the-sensitivity-of-l-type-ca-2-channel-to-inhibition-by-verapamil-in-a-mouse-heart-model
#18
Hironori Sagawa, Shinsuke Hoshino, Kengo Yoshioka, Wei-Guang Ding, Mariko Omatsu-Kanbe, Masao Nakagawa, Yoshihiro Maruo, Hiroshi Matsuura
BACKGROUND: In the clinical setting, verapamil is contraindicated in neonates and infants because of the perceived risk of hypotension or bradyarrhythmia. However, it remains unclear whether there is an age-dependent difference in the sensitivity of cardiac L-type Ca2+ channel current (ICa,L ) to inhibition by verapamil. METHODS: Ventricular myocytes were enzymatically dissociated from the hearts of 6 different age groups (0, 7, 14, 21, 28 days and 10-15 weeks) of mice using a similar Langendorff-perfusion method...
March 19, 2018: Pediatric Research
https://www.readbyqxmd.com/read/29553490/electrophysiological-assessment-of-murine-atria-with-high-resolution-optical-mapping
#19
Kensuke Ihara, Koji Sugiyama, Kentaro Takahashi, Masahiro Yamazoe, Tetsuo Sasano, Tetsushi Furukawa
Recent genome-wide association studies targeting atrial fibrillation (AF) have indicated a strong association between the genotype and electrophysiological phenotype in the atria. That encourages us to utilize a genetically-engineered mouse model to elucidate the mechanism of AF. However, it is difficult to evaluate the electrophysiological properties in murine atria due to their small size. This protocol describes the electrophysiological evaluation of atria using an optical mapping system with a high temporal and spatial resolution in Langendorff perfused murine hearts...
February 22, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29538014/bioengineering-cardiac-tissue-constructs-with-adult-rat-cardiomyocytes
#20
Ze-Wei Tao, Mohamed Mohamed, Jeffrey G Jacot, Ravi K Birla
Bioengineering cardiac tissue constructs with adult cardiomyocytes may help treat adult heart defects and injury. In this study, we fabricated cardiac tissue constructs by seeding adult rat cardiomyocytes on a fibrin gel matrix and analyzed the electromechanical properties of the formed cardiac tissue constructs. Adult rat cardiomyocytes were isolated with a collagenase type II buffer using an optimized Langendorff perfusion system. Cardiac tissue constructs were fabricated using either indirect plating with cardiomyocytes that were cultured for 1 week and dedifferentiated or with freshly isolated cardiomyocytes...
March 13, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
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