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https://www.readbyqxmd.com/read/29245156/selection-and-targeting-of-epcam-protein-by-ssdna-aptamer
#1
Walhan Alshaer, Nida Ababneh, Mamon Hatmal, Heba Izmirli, Moujab Choukeife, Alaa Shraim, Nour Sharar, Aya Abu-Shiekah, Fadwa Odeh, Abeer Al Bawab, Abdalla Awidi, Said Ismail
Aptamers are molecules that reveal highly complex and refined molecular recognition properties. These molecules are capable of binding with high affinity and selectivity to targets, ranging from small molecules to whole living cells. Several aptamers have been selected for targeting cellular proteins and they have also used in developing therapeutics and diagnostic strategies. Epithelial cell adhesion molecule (EpCAM) is considered as a cancer stem cell (CSC) biomarker and one of the most promising targets for aptamer selection against CSCs...
2017: PloS One
https://www.readbyqxmd.com/read/29245123/the-gdnf-family-a-role-in-cancer
#2
REVIEW
Graeme C Fielder, Teresa Wen-Shan Yang, Mahalakshmi Razdan, Yan Li, Jun Lu, Jo K Perry, Peter E Lobie, Dong-Xu Liu
The glial cell line-derived neurotrophic factor (GDNF) family of ligands (GFLs) comprising of GDNF, neurturin, artemin, and persephin plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis. Here we review our current understanding of GFL biology, and supported by recent progress in the area, we examine their emerging role in endocrine-related and other non-hormone-dependent solid neoplasms. The ability of GFLs to elicit actions that resemble those perturbed in an oncogenic phenotype, alongside mounting evidence of GFL involvement in tumor progression, presents novel opportunities for therapeutic intervention...
December 12, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29245122/reduction-of-cancer-cell-viability-by-synergistic-combination-of-photodynamic-treatment-with-the-inhibition-of-the-id-protein-family
#3
Cornelia Roschger, Thomas Verwanger, Barbara Krammer, Chiara Cabrele
The inhibitor of DNA binding and cell differentiation (Id) proteins are dominant negative regulators of the helix-loop-helix transcription factor family and play a key role during development as well as in vascular disorders and cancer. In fact, impairing the Id-protein activity in cancer cells reduces cell growth and even chemoresistance. Recently, we have shown that a synthetic Id-protein ligand (1Y) consisting of a cyclic nonapeptide can reduce the viability of the two breast cancer cell lines MCF-7 and T47D and of the bladder cancer cells T24 to about 50% at concentrations ≥100μM...
December 5, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29245120/bioactive-polyoxygenated-seco-cyclohexenes-from-artabotrys-hongkongensis
#4
Yan-Ping Liu, Jin-Ying Tang, Yan Hua, Liang Lai, Xiu-Lan Luo, Zhi-Jie Zhang, Wen-Qing Yin, Guang-Ying Chen, Yan-Hui Fu
Six new polyoxygenated seco-cyclohexenes, artahongkongenes A-F (1-6), together with six known analogues (7-12) were isolated from the stems and leaves of Artabotrys hongkongensis. Their structures were elucidated by extensive spectroscopic methods. All new compounds were evaluated for their cytotoxicities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro. New seco-cyclohexenes 1-6 showed significant inhibitory effects against various human cancer cell lines with IC50 values ranging from 0...
December 7, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29245105/autoimmune-encephalitis-with-gabaa-receptor-antibodies-in-a-10-year-old-girl
#5
Magdalena Figlerowicz, Paweł Kemnitz, Anna Mania, Katarzyna Mazur-Melewska, Emila Tomczak, Karolina Kuls, Katarzyna Jończyk-Potoczna, Francesc Graus, Wojciech Służewski
Recent studies have indicated that some cases of nonparaneoplastic autoimmune encephalitis in children can be caused by a systemic autoimmune disorder that generates autoantibodies to cell membrane proteins. We describe the clinical features of a 10-year-old girl with autoimmune encephalitis with autoantibodies against the GABAA receptor in whom type 1 diabetes mellitus developed during the course of the disease. The diagnosis was based on the progressive course of disease, pleocytosis in the cerebrospinal fluid (CSF), inflammatory changes in the brain and autoantibodies against the GABAA receptor detected in serum (absent in CSF)...
December 12, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29245078/analysis-of-nras-gain-in-657-patients-with-melanoma-and-evaluation-of-its-sensitivity-to-a-mek-inhibitor
#6
Junya Yan, Xiaowen Wu, Jiayi Yu, Huan Yu, Tianxiao Xu, Kevin M Brown, Xue Bai, Jie Dai, Meng Ma, Huan Tang, Lu Si, Zhihong Chi, Xinan Sheng, Chuanliang Cui, Yan Kong, Jun Guo
BACKGROUND: Neuroblastoma rat-sarcoma (NRAS) mutations have been described in Chinese patients with melanoma. However, the status and the clinical significance of NRAS gain have not been investigated on a large scale. METHODS: A total of 657 melanoma samples were included in the study. NRAS copy number was examined using the QuantiGene Plex DNA assay. The sensitivities of cell lines and patient-derived xenograft (PDX) models containing NRAS gain to a MAP/ERK kinase (MEK) inhibitor (binimetinib) were also evaluated...
December 11, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/29245070/blood-brain-barrier-permeability-and-neuroprotective-effects-of-three-main-alkaloids-from-the-fruits-of-euodia-rutaecarpa-with-mdck-phamdr-cell-monolayer-and-pc12-cell-line
#7
Yi-Nan Zhang, Yan-Fang Yang, Xiu-Wei Yang
The fruits of Euodia rutaecarpa (Euodiae Fructus, EF), the widely used traditional Chinese medicine, have various central nervous system effects. Alkaloids following as evodiamine (EDM), rutaecarpine (RCP) and dehydroevodiamine (DEDM) are the major substances in EF. The MDCK-pHaMDR cell monolayer model was utilized as a blood-brain barrier (BBB) surrogate model to study their BBB permeability. The transport samples were analyzed by high performance liquid chromatography and the apparent permeability coefficients (Papp) were calculated...
December 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29245062/comparative-studies-on-dna-binding-and-in-vitro-antitumor-activity-of-enantiomeric-ruthenium-ii-complexes
#8
Wen-Xu Hong, Fengwen Huang, Tianwen Huan, Xu Xu, Qingguo Han, Gaofeng Wang, Hong Xu, Shan Duan, Yongheng Duan, Xun Long, Ying Liu, Zhangli Hu
A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)2PBIP]2+ {bpy=2,2'-bipyridine, PBIP=2-(4-bromophenyl)imidazo[4,5-f]1,10-phenanthroline} have been synthesized and characterized. The systematic comparative studies between two enantiomers on their DNA binding-behaviors with calf thymus DNA (CT DNA) were carried out by viscosity measurements, spectrophotometric methods and molecular simulation technology. Additional assays were performed to explore the cytotoxicity of the ruthenium(II) enantiomers against tumor cell lines...
November 29, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29245051/magnesium-phosphate-ceramics-incorporating-a-novel-indene-compound-promote-osteoblast-differentiation-in%C3%A2-vitro-and-bone-regeneration-in%C3%A2-vivo
#9
Ju Ang Kim, Hui-Suk Yun, Young-Ae Choi, Jung-Eun Kim, So-Young Choi, Tae-Geon Kwon, Young Kyung Kim, Tae-Yub Kwon, Myung Ae Bae, Nak Jeong Kim, Yong Chul Bae, Hong-In Shin, Eui Kyun Park
Incorporating bioactive molecules into synthetic ceramic scaffolds is challenging. In this study, to enhance bone regeneration, a magnesium phosphate (MgP) ceramic scaffold was incorporated with a novel indene compound, KR-34893. KR-34893 induced the deposition of minerals and expression of osteoblast marker genes in primary human bone marrow mesenchymal stem cells (BMSCs) and a mouse osteoblastic MC3T3-E1 cell line. Analysis of the mode of action showed that KR-34893 induced the phosphorylation of MAPK/extracellular signal-regulated kinase and extracellular signal-regulated kinase, and subsequently the expression of bone morphogenetic protein 7, accompanied by SMAD1/5/8 phosphorylation...
December 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/29245011/oncogenic-role-of-thor-a-conserved-cancer-testis-long-non-coding-rna
#10
Yasuyuki Hosono, Yashar S Niknafs, John R Prensner, Matthew K Iyer, Saravana M Dhanasekaran, Rohit Mehra, Sethuramasundaram Pitchiaya, Jean Tien, June Escara-Wilke, Anton Poliakov, Shih-Chun Chu, Sahal Saleh, Keerthana Sankar, Fengyun Su, Shuling Guo, Yuanyuan Qiao, Susan M Freier, Huynh-Hoa Bui, Xuhong Cao, Rohit Malik, Timothy M Johnson, David G Beer, Felix Y Feng, Weibin Zhou, Arul M Chinnaiyan
Large-scale transcriptome sequencing efforts have vastly expanded the catalog of long non-coding RNAs (lncRNAs) with varying evolutionary conservation, lineage expression, and cancer specificity. Here, we functionally characterize a novel ultraconserved lncRNA, THOR (ENSG00000226856), which exhibits expression exclusively in testis and a broad range of human cancers. THOR knockdown and overexpression in multiple cell lines and animal models alters cell or tumor growth supporting an oncogenic role. We discovered a conserved interaction of THOR with IGF2BP1 and show that THOR contributes to the mRNA stabilization activities of IGF2BP1...
December 14, 2017: Cell
https://www.readbyqxmd.com/read/29244827/neuroligin-4x-overexpression-in-human-breast-cancer-is-associated-with-poor-relapse-free-survival
#11
Henry J Henderson, Balasubramanyam Karanam, Rajeev Samant, Komal Vig, Shree R Singh, Clayton Yates, Deepa Bedi
The molecular mechanisms involved in breast cancer progression and metastasis still remain unclear to date. It is a heterogeneous disease featuring several different phenotypes with consistently different biological characteristics. Neuroligins are neural cell adhesion molecules that have been implicated in heterotopic cell adhesion. In humans, alterations in neuroligin genes are implicated in autism and other cognitive diseases. Until recently, neuroligins have been shown to be abundantly expressed in blood vessels and also play a role implicated in the growth of glioma cells...
2017: PloS One
https://www.readbyqxmd.com/read/29244783/using-a-commonly-down-regulated-cytomegalovirus-cmv-promoter-for-high-level-expression-of-ectopic-gene-in-a-human-b-lymphoma-cell-line
#12
Xiaojun Yu, Wei Geng, Hongchuan Zhao, Guobin Wang, Yijun Zhao, Zhe Zhu, Xiaoping Geng
BACKGROUND Vectors are widely used to drive gene expression using a promoter. However, not all promoters are able to drive ectopic gene expression efficiently, including CMV promoter. Here, we report our data using CMV promoter for high-level gene expression in a B lymphoma cell line DG75. MATERIAL AND METHODS A plasmid (pcDNA3.1(+)) containing the CD21 gene driven under CMV promoter was constructed. The plasmid was stably transfected into a human B lymphoma cell line DG75 for cellular surface CD21 expression, and flow cytometry was used to monitor CD21 expression...
December 15, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29244729/comparative-evaluation-of-u-s-brand-and-generic-intravenous-sodium-ferric-gluconate-complex-in-sucrose-injection-in-vitro-cellular-uptake
#13
Min Wu, Dajun Sun, Katherine Tyner, Wenlei Jiang, Rodney Rouse
Iron deficiency anemia is a common clinical consequence for people who suffer from chronic kidney disease, especially those requiring dialysis. Intravenous (IV) iron therapy is a widely accepted safe and efficacious treatment for iron deficiency anemia. Numerous IV iron drugs have been approved by U.S. Food and Drug Administration (FDA), including a single generic product, sodium ferric gluconate complex in sucrose. In this study, we compared the cellular iron uptake profiles of the brand (Ferrlecit®) and generic sodium ferric gluconate (SFG) products...
December 15, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29244724/rlj-rgd3-suppresses-the-growth-of-heya8-cells-in-nude-mice
#14
Yuanyuan Zheng, Li Lv, Longda Yi, Rui Wu, Rong Xiao, Jihong Wang
In the previous study, rLj-RGD3, a recombinant toxin protein which contains three RGD motifs, was reported to not only inhibit the proliferation of an ovarian cancer cell line, HeyA8 cells, by inducing apoptosis, but also block their adhesion, migration and invasion processes. However, whether rLj-RGD3 could also suppress the tumor growth in HeyA8 xenografted mice has not been reported yet. In the present study, rLj-RGD3 was intraperitoneally injected in the nude mice bearing HeyA8 tumors. Compared with the control group (normal saline), rLj-RGD3 inhibited the tumor growth significantly in the HeyA8 xenografted mice in a dose-dependent manner without affecting their body weights...
December 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29244711/flavonoids-and-ellagitannins-characterization-antioxidant-and-cytotoxic-activities-of-phyllanthus-acuminatus-vahl
#15
Mirtha Navarro, Ileana Moreira, Elizabeth Arnaez, Silvia Quesada, Gabriela Azofeifa, Felipe Vargas, Diego Alvarado, Pei Chen
The phenolic composition of leaves from Phyllanthus acuminatus L., a plant commonly used in Costa Rica as traditional medicine, was studied using UPLC-ESI-MS on an enriched phenolic extract. A total of 20 phenolic compounds were identified, comprising eight flavonoids (two flavanones-pinocembrin isomers and six derivatives from apigenin, chrysin, quercetin, and kaempferol); seven ellagitannins, two flavan-3-ols (prodelphinidin B dimer and (epi)gallocatechin); and three phenolic acids (ellagic acid, trimethylellagic acid, and ferulic acid)...
December 15, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29244610/inhibition-of-exosome-release-by-ketotifen-enhances-sensitivity-of-cancer-cells-to-doxorubicin
#16
Farman Matloob Khan, Ekram Saleh, Hussain Alawadhi, Rania Harati, Wolfram-Hubertus Zimmermann, Raafat El-Awady
Exosomes released from cancer cells support metastasis and growth of recipient cells and increase their resistance to chemotherapy. Therapeutic targeting of exosomes is a promising area in cancer research. Our aim is to test the effect of the mast cell stabilizer ketotifen on exosomes release from cancer cells and how this can modify their response to doxorubicin. Exosomes release from three cancer cell lines (MCF7, HeLa and BT549) was assessed by scan electron microscope and exosomes quantification kit. Doxorubicin export within exosomes was monitored flurometrically and cellular sensitivity to doxorubicin ± ketotifen was measured by sulphorhodamine-B and colony formation assays...
December 15, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29244495/antiprotozoal-sesquiterpene-lactones-and-other-constituents-from-tarchonanthus-camphoratus-and-schkuhria-pinnata
#17
Njogu M Kimani, Josphat C Matasyoh, Marcel Kaiser, Reto Brun, Thomas J Schmidt
In continuation of a search for new antiprotozoal agents from plants of the family Asteraceae, Tarchonanthus camphoratus and Schkuhria pinnata have been investigated. By following the promising in vitro activity of the dichloromethane extracts from their aerial parts, bioassay-guided chromatographic isolation yielded two known sesquiterpene lactones (1 and 2) from T. camphoratus and 20 known compounds of this type from S. pinnata. From the latter, a new eudesmanolide, (1R*,5S*,6R*,7R*,8R*,10R*)-1-hydroxy-8-[5″-hydroxy-4'-(2″-hydroxyisovaleroyloxy)tigloyloxy]-3-oxoeudesma-11(13)-en-6,12-olide (3), and two new germacranolides, 3β-(2″-hydroxyisovaleroyloxy)-8β-(3-furoyloxy)costunolide (14) and 1(10)-epoxy-3β-hydroxy-8β-[5'-hydroxy-4'-(2″-hydroxyisovaleroyloxy)tigloyloxy]costunolide (16), were obtained...
December 15, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29244209/generation-of-a-kshv-k13-deletion-mutant-for-vflip-function-study
#18
Fei Wang, Yuanyuan Guo, Wan Li, Chun Lu, Qin Yan
Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded viral Fas-associated death domain-like IL-1-converting enzyme inhibitory protein (vFLIP) is one of the latently expressed genes and plays a key role in cell survival and maintenance of latent infection by activating the NF-κB pathway. To obtain a genetic system for studying KSHV vFLIP mutation in the context of the viral genome, we generated recombinant viruses lacking the coding sequence (CDS) of vFLIP gene (K13/ORF71) by bacterial artificial chromosome (BAC) technology and the Escherichia coli Red recombination system...
December 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29244195/decellularized-colorectal-cancer-matrix-as-bioactive-microenvironment-for-in-vitro-3d-cancer-research
#19
Martina Piccoli, Edoardo D'Angelo, Sara Crotti, Francesca Sensi, Luca Urbani, Edoardo Maghin, Alan Burns, Paolo De Coppi, Matteo Fassan, Massimo Rugge, Flavio Rizzolio, Antonio Giordano, Pierluigi Pilati, Enzo Mammano, Salvatore Pucciarelli, Marco Agostini
Three-dimensional (3D) cancer models are overlooking the scientific landscape with the primary goal of bridging the gaps between two-dimensional (2D) cell lines, animal models and clinical research. Here we describe an innovative tissue engineering approach applied to colorectal cancer (CRC) starting from decellularized human biopsies in order to generate an organotypic 3D-bioactive model. This in vitro 3D system recapitulates the ultrastructural environment of native tissue as demonstrated by histology, immunohistochemistry, immunofluorescence and scanning electron microscopy analyses...
December 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29244184/accumulation-of-long-term-transcriptionally-active-integrated-retroviral-vectors-in-active-promoters-and-enhancers
#20
Filip Šenigl, Dalibor Miklík, Miroslav Auxt, Jirí Hejnar
Most retroviruses preferentially integrate into certain genomic locations and, as a result, their genome-wide integration patterns are non-random. We investigate the epigenetic landscape of integrated retroviral vectors and correlate it with the long-term stability of proviral transcription. Retroviral vectors derived from the avian sarcoma/leukosis virus expressing the GFP reporter were used to transduce the human myeloid lymphoblastoma cell line K562. Because of efficient silencing of avian retrovirus in mammalian cells, only ∼3% of established clones displayed stable proviral expression...
December 15, 2017: Nucleic Acids Research
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