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https://www.readbyqxmd.com/read/29054095/ginkgolide-b-and-bilobalide-ameliorate-neural-cell-apoptosis-in-%C3%AE-synuclein-aggregates
#1
Jun Hua, Nuo Yin, Beibei Yang, Ji Zhang, Jianhua Ding, Yi Fan, Gang Hu
The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the α-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human α-Syn, but not α-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay...
October 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29054047/application-of-transglycosylated-stevia-and-hesperidin-as-drug-carriers-to-enhance-biopharmaceutical-properties-of-poorly-soluble-artemisinin
#2
Kumaran Letchmanan, Shou-Cang Shen, Wai Kiong Ng, Reginald B H Tan
Biopharmaceutical properties of poorly water-soluble antimalarial drug, Artemisinin (ART), were improved by formulating amorphous solid dispersions with transglycosylated food additives (Hsp-G and Stevia-G) via co-spray drying. Both the formulated ART/Hsp-G and ART/Stevia-G showed superior dissolution properties with a burst release of more than 95% of drug within 5 min, whereas untreated ART dissolved only 4% in 5min. The supersaturation solubility of the formulated ART was enhanced by 2-fold as compared with untreated counterpart...
October 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29054027/sapofectosid-ensuring-non-toxic-and-effective-dna-and-rna-delivery
#3
Simko Sama, Gerold Jerz, Peter Schmieder, Eric Woith, Matthias F Melzig, Alexander Weng
Different methods are being deployed for non-viral DNA/RNA delivery. However non-viral formulations for DNA/RNA-delivery are often accompanied by severe toxicity and thus low efficiency. Particular costly cell culture media are required as well. Here we introduce sapofection as a valuable enhancing method for non-viral DNA/RNA delivery. Sapofection is based on the application of DNA/RNA nanoplexes and sapofectosid, a plant derived natural transfection reagent. Sapofectosid was produced from plant raw material by chromatographic methods and characterized by tandem mass spectrometry and intensive one and two dimensional NMR-spectroscopy...
October 17, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29053994/dietary-resveratrol-confers-apoptotic-resistance-to-oxidative-stress-in-myoblasts
#4
Satoshi Haramizu, Shinichi Asano, David C Butler, David A Stanton, Ameena Hajira, Junaith S Mohamed, Stephen E Alway
High levels of reactive oxygen species (ROS) contribute to muscle cell death in aging and disuse. We have previously found that resveratrol can reduce oxidative stress in response to aging and hindlimb unloading in rodents in vivo, but it was not known if resveratrol would protect muscle stem cells during repair or regeneration when oxidative stress is high. To test the protective role of resveratrol on muscle stem cells directly, we treated the C2C12 mouse myoblast cell line with moderate (100 μM) or very high (1 mM) levels of H2O2 in the presence or absence of resveratrol...
August 24, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29053955/the-met1-linked-ubiquitin-machinery-emerging-themes-of-de-regulation
#5
REVIEW
Matous Hrdinka, Mads Gyrd-Hansen
The linear ubiquitin chain assembly complex, LUBAC, is the only known mammalian ubiquitin ligase that makes methionine 1 (Met1)-linked polyubiquitin (also referred to as linear ubiquitin). A decade after LUBAC was discovered as a cellular activity of unknown function, there are now many lines of evidence connecting Met1-linked polyubiquitin to NF-κB signaling, cell death, inflammation, immunity, and cancer. We now know that Met1-linked polyubiquitin has potent signaling functions and that its deregulation is connected to disease...
October 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29053932/the-patterns-of-in-vitro-cell-death-and-inflammatory-cytokines-induced-by-distinct-bcg-vaccine-strains-are-differentially-induced-in-human-mononuclear-cells
#6
C Ponte, M Hacker, M Moraes, L Castello-Branco, F Silva, P Antas
Tuberculosis (TB) remains among the world's leading cause of mortality. For its control, studies of TB vaccines are needed. Since live-attenuated Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only TB vaccine currently in use, studies on the protective role of BCG are required. In this study, we analyzed host cells purified directly from whole blood of human immunodeficiency virus (HIV)-negative volunteers, comprising adult healthy donors (HD) and neonates (umbilical cord bloods, UCB), with the aim to directly compare in vitro immune responses with distinct BCG strains in human mononuclear cells...
October 20, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29053864/damage-associated-responses-of-the-host-contribute-to-defence-against-cyst-nematodes-but-not-root-knot-nematodes
#7
Syed Jehangir Shah, Muhammad Shahzad Anjam, Badou Mendy, Muhammad Arslan Anwer, Samer S Habash, Jose L Lozano-Torres, Florian M W Grundler, Shahid Siddique, Robert Hancock
When nematodes invade and subsequently migrate within plant roots, they generate cell wall fragments (in the form of oligogalacturonides; OGs) that can act as damage-associated molecular patterns and activate host defence responses. However, the molecular mechanisms mediating damage responses in plant-nematode interactions remain unexplored. Here, we characterized the role of a group of cell wall receptor proteins in Arabidopsis, designated as polygalacturonase-inhibiting proteins (PGIPs), during infection with the cyst nematode Heterodera schachtii and the root-knot nematode Meloidogyne incognita...
October 19, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29053790/the-hippo-signaling-pathway-in-pancreatic-%C3%AE-cells-functions-and-regulations
#8
Amin Ardestani, Kathrin Maedler
Hippo signaling is an evolutionarily conserved pathway that critically regulates development and homeostasis of various tissues in response to a wide range of extracellular and intracellular signals. As an emerging significant player in many diseases, the Hippo pathway is also involved in the pathophysiology of diabetes on the level of the pancreatic islets. Multiple lines of evidence uncover the importance of Hippo signaling in pancreas development as well as in the regulation of β-cell survival, proliferation and regeneration...
October 18, 2017: Endocrine Reviews
https://www.readbyqxmd.com/read/29053745/impact-of-the-structural-integrity-of-the-three-way-junction-of-adenovirus-vai-rna-on-pkr-inhibition
#9
Edis Dzananovic, Astha, Grzegorz Chojnowski, Soumya Deo, Evan P Booy, Pauline Padilla-Meier, Kevin McEleney, Janusz M Bujnicki, Trushar R Patel, Sean A McKenna
Highly structured RNA derived from viral genomes is a key cellular indicator of viral infection. In response, cells produce the interferon inducible RNA-dependent protein kinase (PKR) that, when bound to viral dsRNA, phosphorylates eukaryotic initiation factor 2α and attenuates viral protein translation. Adenovirus can evade this line of defence through transcription of a non-coding RNA, VAI, an inhibitor of PKR. VAI consists of three base-paired regions that meet at a three-way junction; an apical stem responsible for the interaction with PKR, a central stem required for inhibition, and a terminal stem...
2017: PloS One
https://www.readbyqxmd.com/read/29053683/new-application-of-an-atmospheric-pressure-plasma-jet-as-a-neuro-protective-agent-against-glucose-deprivation-induced-injury-of-sh-sy5y-cells
#10
Xu Yan, Zhaozhong Meng, Jiting Ouyang, Yajun Qiao, Fang Yuan
The atmospheric pressure plasma jet (APPJ) has attracted the attention of many researchers from multiple disciplines in recent years because its emissions include multiple types of reactive nitrogen species (RNS) and reactive oxygen species (ROS). Our previous study has shown the cytoprotective effect of the APPJ against oxidative stress-induced injuries. The aim of the present study is to provide a detailed in vitro treatment protocol regarding the neuroprotective applications of helium APPJs on glucose deprivation-induced injury in SH-SY5Y cells...
October 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053667/assay-development-for-high-content-quantification-of-sod1-mutant-protein-aggregate-formation-in-living-cells
#11
Honggun Lee, Constantin Radu, Jeung Whan Han, Regis Grailhe
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that can be caused by inherited mutations in the gene encoding copper-zinc superoxide dismutase 1 (SOD1). The structural instability of SOD1 and the detection of SOD1-positive inclusions in familial-ALS patients supports a potential causal role for misfolded and/or aggregated SOD1 in ALS pathology. In this study, we describe the development of a cell-based assay designed to quantify the dynamics of SOD1 aggregation in living cells by high content screening approaches...
October 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053567/eco-friendly-formulated-zinc-oxide-nanoparticles-induction-of-cell-cycle-arrest-and-apoptosis-in-the-mcf-7-cancer-cell-line
#12
Amin Boroumand Moghaddam, Mona Moniri, Susan Azizi, Raha Abdul Rahim, Arbakariya Bin Ariff, Mohammad Navaderi, Rosfarizan Mohamad
Green products have strong potential in the discovery and development of unique drugs. Zinc oxide nanoparticles (ZnO NPs) have been observed to have powerful cytotoxicity against cells that cause breast cancer. The present study aims to examine the cell cycle profile, status of cell death, and pathways of apoptosis in breast cancer cells (MCF-7) treated with biosynthesized ZnO NPs. The anti-proliferative activity of ZnO NPs was determined using MTT assay. Cell cycle analysis and the mode of cell death were evaluated using a flow cytometry instrument...
October 20, 2017: Genes
https://www.readbyqxmd.com/read/29053414/perk-phosphorylated-eif2%C3%AE-pathway-suppresses-tumor-metastasis-through-downregulating-expression-of-programmed-death-ligand-1-and-cxcl5-in-triple-negative-breast-cancer
#13
Weiwei Zou, Yu Bai, Xilong Wang, Kai Cheng, Hongguang Sun, Guoqiang Zhang, Xiaohong Wang, Zhenlin Yang
Endoplasmic reticulum (ER) stress has been reported to be associated with metastasis in many malignant tumors. PKR-like ER kinase-phosphorylated eukaryotic translation initiation factor 2α (PERK-p-eIF2α) pathway is one of the three main signal pathways in ER stress, however, its mechanism in regulating breast cancer (BC) relapse or metastasis was still not completely understood. Besides, drug resistance was an important factor influencing the effect of tumor treatment and whether PERK-p-eIF2α pathway was involved in the drug resistance to BC treatment also needs to be explored...
October 2017: Cancer Biotherapy & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29053271/discovery-of-7-18-f-fluorotryptophan-as-a-novel-pet-probe-for-the-visualization-of-tryptophan-metabolism-in-vivo
#14
Boris D Zlatopolskiy, Johannes Zischler, Dominique Schäfer, Elizaveta A Urusova, Mehrab Guliyev, Olesia Bannykh, Heike Endepols, Bernd Neumaier
Tryptophan and its metabolites are involved in different physiological and pathophysiological processes. Consequently, PET tracers addressing tryptophan metabolic pathways should allow the detection of different pathologies like neurological disorders and cancer. Herein we report an efficient method for the preparation of fluorotryptophans labeled in different positions with (18)F and their biological evaluation. 4-7-[(18)F]Fluorotryptophans ([(18)F]FTrps) were prepared according to a modified protocol of alcohol-enhanced Cu-mediated radiofluorination in 30-53% radiochemical yields...
October 20, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29053266/structure-activity-relationships-of-new-natural-product-based-diaryloxazoles-with-selective-activity-against-androgen-receptor-positive-breast-cancer-cells
#15
Andrew J Robles, Shelby McCowen, Shengxin Cai, Michaels Glassman, Francisco Ruiz, Robert H Cichewicz, Stanton F McHardy, Susan L Mooberry
Targeted therapies for ER+/PR+ and HER2-amplified breast cancers have improved patient survival, but there are no therapies for triple negative breast cancers (TNBC) that lack expression of estrogen and progesterone receptors (ER/PR), or amplification or overexpression of HER2. Gene expression profiling of TNBC has identified molecular subtypes and representative cell lines. An extract of the Texas native plant Amyris texana was found to have selective activity against MDA-MB-453 cells, a model of the luminal androgen receptor (LAR) subtype of TNBC...
October 20, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29053264/cytotoxic-half-sandwich-rh-iii-and-ir-iii-%C3%AE-diketonates
#16
Agnese Petrini, Riccardo Pettinari, Fabio Marchetti, Claudio Pettinari, Bruno Therrien, Agustı N Galindo, Rosario Scopelliti, Tina Riedel, Paul J Dyson
A series of half-sandwich pentamethylcyclopentadienyl rhodium(III) and iridium(III) complexes [Cp*M(DBM/HDB/AVB)Cl] and [Cp*M(DBM/HDB/AVB)(PTA)][SO3CF3], where Cp* = pentamethylcyclopentadienyl, the proligands DBMH = dibenzoylmethane, HDBH = o-hydroxydibenzoylmethane, AVBH = avobenzone, and PTA = 1,3,5-triaza-7-phosphaadamantane, is reported. All the complexes were characterized by IR, (1)H and (13)C NMR spectroscopy, electrospray ionization mass spectrometry, elemental analysis, and DFT calculations. Five of the complexes have also been characterized in the solid-state by X-ray crystallography...
October 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29053187/xist-promotes-gastric-cancer-gc-progression-through-tgf-%C3%AE-1-via-targeting-mir-185
#17
Quan Zhang, Baiyu Chen, Ping Liu, Jing Yang
LncRNAs and microRNAs can play significant roles in various cancers, including gastric cancer (GC). In our study, we investigated the role of lncRNA XIST in GC. We observed that XIST was increased in MGC803 and BGC823 cells compared to human normal gastric epithelial GES-1 cells. It was also shown that miR-185 was decreased in GC cell lines. Silencing XIST can inhibit the growth of GC cells and bioinformatics analysis was performed to confirm the correlation between XIST and miR-185. Interestingly, a negative correlation was indicated between XIST and miR-185 in GC cells...
October 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29052968/non-steroidal-estrogen-receptor-isoform-selective-biphenyls
#18
Seema Bhatnagar, Anjali Soni, Swati Kaushik, Megha Rikhi, T R Santhosh Kumar, B Jayaram
Estrogen receptor (ER) has been a therapeutic target to treat ER positive breast cancer, most notably by agents known as selective estrogen receptor modulators (SERMs). However, resistance and severe adverse effects of known drugs gave impetus to the search for newer agents with better therapeutic profile. ERα and ERβ are two isoforms sharing 56% identity, and having different physiological functions and expressions in various tissues. Only two residues differ in the active sites of the two isoforms motivating us to design isoform selective ligands...
October 20, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29052919/oamdp-a-novel-podophyllotoxin-derivative-induces-apoptosis-cell-cycle-arrest-and-autophagy-in-hepatoma-hepg2-cells
#19
Jie Ren, Yu Liu, Lixia Li, Yuexin Zhao, Zhongyu Li, Chao Wu, Lin Chen, Kun Hu
4β-(1,3,4-oxadiazole-2-amino-5-methyl)-4-deoxypodophyllotoxin (OAMDP), a novel podophyllotoxin derivative, has demonstrated potent anti-tumor activity with significant cytotoxic effect. Here we report the anti-proliferative effect of OAMDP, for which OAMDP could suppress the proliferation of HepG2 cells (human hepatoma cell line) in a dose- and time-dependent manner. After treating with OAMDP, cell apoptosis was confirmed by Annexin V-FITC/PI double staining assay. Furthermore, flow cytometry analysis revealed the loss of mitochondrial membrane potential and the increase of intracellular reactive oxygen species in HepG2 cells...
October 20, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29052817/androgen-signaling-in-esophageal-adenocarcinoma-cell-lines-in-vitro
#20
Helen M Palethorpe, Paul A Drew, Eric Smith
BACKGROUND: We showed previously that nuclear localization of the androgen receptor (AR) and expression of the androgen-responsive gene FK506-binding protein 5 (FKBP5) in esophageal adenocarcinoma (EAC) tissues were associated with decreased patient survival, suggesting a role for androgens in this cancer. AIM: To investigate the effect of the AR ligand 5α-dihydrotestosterone (DHT) on AR-expressing EAC cell lines in vitro. METHODS AND RESULTS: In tissue resection specimens from EAC patients, FKBP5 expression was positively associated with proliferation as measured by Ki-67 expression...
October 20, 2017: Digestive Diseases and Sciences
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