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Ali Al-Ahmad, Aleksander Walankiewicz, Elmar Hellwig, Marie Follo, Christian Tennert, Annette Wittmer, Lamprini Karygianni
Alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. Antimicrobial photodynamic therapy (aPDT) has recently gained attention as a novel method to eradicate pathogens. The aim of this study was to evaluate the antimicrobial effects of a novel aPDT method using visible light (vis) and water infiltrated infrared A (wIRA) in combination with chlorine e6 (Ce6) against different periodontal pathogens in planktonic form and within in situ subgingival oral biofilms...
2016: Frontiers in Microbiology
Fernando H Biase, Chanaka Rabel, Michel Guillomot, Isabelle Hue, Kalista Andropolis, Colleen A Olmstead, Rosane Oliveira, Richard Wallace, Daniel Le Bourhis, Christophe Richard, Evelyne Campion, Aurélie Chaulot-Talmon, Corinne Giraud-Delville, Géraldine Taghouti, Hélène Jammes, Jean-Paul Renard, Olivier Sandra, Harris A Lewin
A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients before or during the implantation window. We investigated this problem by using RNA sequencing (RNA-seq) to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at day (d) 18 (preimplantation) and d 34 (postimplantation) of gestation. In addition, endometrium was profiled to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before or during the implantation window...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
Fotios Tzerbos, Fotios Bountaniotis, Nadia Theologie-Lygidakis, Dimitrios Fakitsas, Ioannis Fakitsas
Zygomatic implants have been used for rehabilitation of the edentulous atrophic maxilla as an alternative to bone grafting for almost two decades resulting in satisfactory clinical outcomes. However, the patients with edentulous atrophic maxilla treated using this technique may present serious complications that could put the prosthetic restoration at risk. Four cases are reported in this paper, one case with a cutaneous fistula in the left zygomatic-orbital area caused by aseptic necrosis at the apical part of the implant, which was treated with the surgical removal of this part, a second case with loss of the right zygomatic implant due to failure of osseointegration and two cases of periimplantitis that resulted in partial and complete removal of the implant, respectively...
September 2016: Acta Stomatologica Croatica
Kuang-Wei Hsu, Yu-Fu Shen, Pein-Chi Wei
STATEMENT OF PROBLEM: In addition to the original abutments provided by implant companies, compatible computer-aided design and computer-aided manufacturing (CAD-CAM) abutments are also available from different manufacturers. However, the combination of abutments and implant systems from different manufacturers may lead to mechanical problems between components. Little has been reported on the clinical performance of this treatment option. PURPOSE: The purpose of this retrospective case series was to evaluate the outcome of compatible CAD-CAM titanium abutments (TiAs) for posterior single-implant tooth replacement (PSITR) up to 6 years after insertion...
October 17, 2016: Journal of Prosthetic Dentistry
Pouria HosseinNia, Mehdi Hajian, Mojtaba Tahmoorespur, Sayyed Morteza Hosseini, Somayyeh Ostadhosseini, Mohammad Reza Nasiri, Mohammad Hossein Nasr-Esfahani
BACKGROUND: Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MATERIALS AND METHODS: In this experimental study, stage-specific profiling using real time-quantitative polymerase chain reaction (RT-qPCR) revealed robust and dynamic patterns of stage-specific gene activity that fall into four major clusters depending on their respective mRNA profiles...
October 2016: International Journal of Fertility & Sterility
Vahid Khoshkam, Fernando Suárez-López Del Amo, Alberto Monje, Guo-Hao Lin, Hsun-Liang Chan, Hom-Lay Wang
PURPOSE: The purpose of this systematic review was to evaluate long-term outcomes of regenerative procedures for treating peri-implantitis. MATERIALS AND METHODS: Electronic searches of five databases and hand searches of journals were performed to identify human trials that had treated more than 10 periimplantitis lesions using a regenerative approach with a follow-up period of at least 36 months. To meet the inclusion criteria, studies had to report at least one of the following parameters: radiographic bone fill (RBF), probing depth (PD) reduction, clinical attachment level gain, bleeding on probing reduction, and mucosal level gain...
November 2016: International Journal of Oral & Maxillofacial Implants
Ausra Ramanauskaite, Gintaras Juodzbalys, Tolga F Tözüm
OBJECTIVES: To review the literature on retrograde periimplantitis symptoms, risk factors, and treatment methods and to propose a decision-making tree of retrograde periimplantitis management. MATERIALS AND METHODS: An electronic literature search was conducted on the MEDLINE and EMBASE databases for articles published between 1990 and 2015. Clinical human studies in the English language were included. RESULTS: The search resulted in 44 case reports published by 27 authors...
October 2016: Implant Dentistry
Paul S Rosen, Stuart J Froum
The biologic complication of peri-implantitis threatens the survival of dental implants that have successfully integrated and are in function. A list of seven risk factors associated with this disease was generated in 2006 at the Sixth European Workshop on Periodontology. These factors were based on the prevailing literature. Since then, modifications to this list have been considered whereby some of the original items suggested to be associated with periimplantitis would be deleted, while, in light of evidence-based information, newer, emerging risks may be included...
July 2016: Compendium of Continuing Education in Dentistry
Juliano Milanezi de Almeida, Henrique Rinaldi Matheus, David Jonathan Rodrigues Gusman, Paula Lazilha Faleiros, Nathália Januário de Araújo, Vivian Cristina Noronha Novaes
OBJECTIVE: This study aimed to perform a systematic review of the effectiveness of nonsurgical treatment associated with different adjuvant therapies on periimplantitis. MATERIALS AND METHODS: Different individuals, following a research process, performed a network research of controlled and randomized controlled clinical trials on PubMed, Embase/MEDLINE, with 20 years' time constraint and the last search in January 2016. RESULTS: From 108 articles found by the first search, they analyzed 10 full texts, and in none did they find a standard control group...
February 2017: Implant Dentistry
Rucha Shah, Raison Thomas, Tarun Kumar, Dhoom Singh Mehta
BACKGROUND: Retrograde periimplantitis (RPI) is the inflammatory disease that affects the apical part of an osseointegrated implant while the coronal portion of the implant sustains a normal bone-to-implant interface. The aim of the current study was to assess the intraexaminer and interexaminer reliability of a proposed new classification system for RPI. MATERIALS AND METHODS: After thorough electronic literature search, 56 intraoral periapical radiographs (IOPA) of implants with RPI were collected and were classified by 2 independent reviewers as per the new classification system into one of the 3-mild, moderate, and advanced-classes based on the amount of bone loss from the apex of the implant to the most coronal part as a percentage of the total implant length...
December 2016: Implant Dentistry
Bernhard Pommer, Robert Haas, Georg Mailath-Pokorny, Rudolf Fürhauser, Georg Watzek, Dieter Busenlechner, Michael Müller-Kern, Claudia Kloodt
PURPOSE: Periimplantitis is the most frequent cause of late implant failure; however, little is known about the long-term success of periimplantitis treatment and the effectiveness of various therapeutic interventions. MATERIALS AND METHODS: A total of 142 patients were referred to the Academy for Oral Implantology in Vienna for the treatment of recurrent periimplantitis around single-tooth implants. Of them, 72 patients (51%) were treated by laser decontamination, 47 patients (33%) by implantoplasty surgery, and 23 patients (16%) by a combination of both approaches...
October 2016: Implant Dentistry
Melissa S Lang, D Roselyn Cerutis, Takanari Miyamoto, Martha E Nunn
PURPOSE: The aim of this study was to evaluate the surface characteristics and gingival fibroblast adhesion of disks composed of implant and abutment materials following brief and repeated instrumentation with instruments commonly used in procedures for implant maintenance, stage-two implant surgery, and periimplantitis treatment. MATERIALS AND METHODS: One hundred twenty disks (40 titanium, 40 titaniumzirconium, 40 zirconia) were grouped into treatment categories of instrumentation by plastic curette, titanium curette, diode microlaser, rotary titanium brush, and no treatment...
July 2016: International Journal of Oral & Maxillofacial Implants
Michael R Norton
INTRODUCTION: Sinus grafts are a safe and predictable treatment for increasing bone volume in the posterior maxilla. Common complications include membrane perforations and postoperative infection. Little is published with regard to late-onset infection except as it relates to periimplantitis. This report presents a case of late sequestration of bovine bone mineral graft fully consolidated within host lamellar bone, 8 years postsinus lift. Histology revealed mature lamellar bone encapsulating well-integrated particles of bovine bone material with evidence of devitalization and marrow spaces filled with a dense neutrophil infiltrate and a filamentous mixed microbial flora...
August 2016: Implant Dentistry
Daehyun Lee, Byungjin Sohn, Kyoung Hwa Kim, Sungtae Kim, Ki-Tae Koo, Tae-Il Kim, Yang-Jo Seol, Yong-Moo Lee, In-Chul Rhyu, Young Ku
BACKGROUND: There have been previous studies on the relationship between periodontitis and peri-implantitis, but limited information is available on how periodontitis affects osseointegration and wound healing of newly placed dental implants adjacent to natural teeth. The objective of the present experiment is to evaluate healing around dental implants adjacent to teeth with untreated experimental periodontitis. METHODS: The study included six male beagle dogs. Scaling and plaque control procedures were performed on three dogs (control group)...
October 2016: Journal of Periodontology
Tomas Albrektsson, Luigi Canullo, David Cochran, Hugo De Bruyn
BACKGROUND: The discrepancy between some scientific views and the daily clinical experience with dental implants has made the topic of "periimplantitis" highly controversial, especially the discussion whether "periimplantitis" should even be considered a "disease" or whether marginal bone loss instead would represent a complication of having a foreign body placed in the oral cavity. PURPOSE: The aim of the present paper was to present the outcomes from a consensus meeting on "peri-implantitis" in Rome, Italy (January 8-10, 2016)...
August 2016: Clinical Implant Dentistry and related Research
Hye-Min Ku, Mi-Kyoung Jun, Jee-Hwan Kim, Ho-Keun Kwon, Baek-Il Kim
INTRODUCTION: It is important to identify the reasons for implant failure when planning future dental treatment. No studies have distinguished the factors that affect implant failure by evaluating the appearance of failed fixtures. PURPOSE: This study investigated these factors by evaluating the surface of implant fixtures using quantitative light-induced fluorescence-digital (QLF-D), which involves detecting the red fluorescence emitted by porphyrin metabolized by oral bacteria in a mature biofilm...
June 2016: Implant Dentistry
Ali Al-Ahmad, Lamprini Karygianni, Max Schulze Wartenhorst, Maria Bächle, Elmar Hellwig, Marie Follo, Kirstin Vach, Jung-Suk Han
Bacterially-driven mucosal inflammation and the development of periimplantitis can lead to oral implant failure. In this study, initial bacterial adhesion after 2 h and biofilm formation after 1 day and 3 days were analyzed in situ on novel 3 mol% yttria-stabilized tetragonal zirconia polycrystal samples (Zr; 3Y-TZP), as well as on alumina and niobium co-doped yttria-stabilized tetragonal zirconia samples (Al-Zr; Al2O3/Y(Nb)-TZP). Pure titanium implant material (Ti) and bovine enamel slabs (BES) served as controls...
April 19, 2016: Journal of Medical Microbiology
Roberto Gonçalves Junior, Aristides da Rosa Pinheiro, José Jorge Schoichet, Carlos Henrique Ramirez Nunes, Rackel Gonçalves, Leticia Ladeira Bonato, Valquiria Quinelato, Leonardo Santos Antunes, Erika Calvano Küchler, Julie Lobo, Ricardo de Mello Villas-Bôas, Alexandre Rezende Vieira, José Mauro Granjeiro, Priscila Ladeira Casado
Subjects susceptible to chronic periodontitis (CP) show a high risk for the development of periimplantitis (PI). Both diseases are multifactorial, presenting similarities in their pathophysiology and polygenic profile. MMP-13 (matrix metalloproteinases 13/ collagenase 3) is a collagenolytic enzyme, which expression is induced by TGF beta 3 (transforming growth factor type 3) in human gingival fibroblasts and inhibited by TIMP-2 (tissue inhibitor of metalloproteinase type 2). The aim of this study was to investigate the occurrence of periimplantitis (PI) in subjects with history of chronic periodontitis (CP) and polymorphisms frequency in MMP13, TIMP2 and TGFB3 genes...
March 2016: Brazilian Dental Journal
Balendra Pratap Singh, Hemant Jivanani
Around 1300 different types of dental implants are available worldwide and the implant manufacturers are resorting to aggressive marketing strategies; claiming their implants to provide a superior outcome. The clinician is left with a constant dilemma on which implant to choose for better clinical outcome and welfare of the patient. Moreover, in India, economical consideration is a concern too. The dentist has to select an implant that provides a good result and is economical. Cochrane systematic reviews provide the gold standard evidence for intervention, diagnosis, etc...
July 2015: Journal of Indian Prosthodontic Society
Shuangbo Kong, Xue Han, Tongtong Cui, Chan Zhou, Yufei Jiang, Hangxiao Zhang, Bingyan Wang, Haibin Wang, Shuang Zhang
Uterine decidualization characterized by stromal cell proliferation and differentiation is critical to the establishment of pregnancy in many species. Progesterone is a key factor in regulating endometrial cell decidualization, however, the molecular basis involved in mediating the effects of progesterone during decidualization remains largely unknown. We report here that the DNA replication licensing factor MCM2, one of the conserved set of six-related proteins (MCM complex: MCM2-7) essential for eukaryotic DNA replication, is dynamically expressed in both proliferative and differentiated stromal cells during mouse periimplantation uterus...
August 2016: Endocrine
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