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https://www.readbyqxmd.com/read/28735481/single-cell-mass-cytometry-of-acute-myeloid-leukemia-and-leukemia-stem-progenitor-cells
#1
Zhihong Zeng, Marina Konopleva, Michael Andreeff
Mass cytometry time-of-flight (CyTOF) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse. Here we provide a protocol for AML sample preparation for CyTOF and keynotes emphasizing critical steps in and troubleshooting strategies for this procedure.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28734901/gastroresistant-oral-peptide-for-fluorescence-imaging-of-colonic-inflammation
#2
Paola Luciani, Ander Estella-Hermoso de Mendoza, Tommaso Casalini, Silvia Lang, Kirstin Atrott, Marianne R Spalinger, Anna Pratsinis, Jens Sobek, Isabelle Frey-Wagner, Jens Schumacher, Jean-Christophe Leroux, Gerhard Rogler
The use of molecular markers for inflammation in the gastrointestinal tract could empower optical imaging modalities for early diagnosis and eventually personalized timely treatments. A major hurdle to the widespread use of functional fluorescence imaging is the absence of suitable contrast agents, in particular to be administered via the oral route due to the usual proteolytic susceptibility of the biomarkers. By designing a retro-inverso peptide, starting from a previously described sequence specific for N-cadherin, we achieved resistance to gastrointestinal degradation and even slightly improved specificity towards the target, both in ex vivo and in vivo experimental colitis...
July 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28734509/global-disability-empowering-children-of-all-abilities
#3
REVIEW
Rebecca J Scharf, Angelina Maphula, Paige C Pullen, Rita Shrestha, Gaynell Paul Matherne, Reeba Roshan, Beena Koshy
Worldwide, children are often not meeting their developmental potential owing to malnutrition, infection, lack of stimulation, and toxic stress. Children with disabilities are more likely to experience poverty, neglect, and abuse, and are less likely to have adequate access to education and medical care. Early childhood developmental stimulation can improve language, learning, and future participation in communities. Therapeutic supports and endeavors to reduce stigma for people of all abilities strengthen communities and allow for human thriving...
August 2017: Pediatric Clinics of North America
https://www.readbyqxmd.com/read/28729679/a-population-specific-reference-panel-empowers-genetic-studies-of-anabaptist-populations
#4
Liping Hou, Rachel L Kember, Jared C Roach, Jeffrey R O'Connell, David W Craig, Maja Bucan, William K Scott, Margaret Pericak-Vance, Jonathan L Haines, Michael H Crawford, Alan R Shuldiner, Francis J McMahon
Genotype imputation is a powerful strategy for achieving the large sample sizes required for identification of variants underlying complex phenotypes, but imputation of rare variants remains problematic. Genetically isolated populations offer one solution, however population-specific reference panels are needed to assure optimal imputation accuracy and allele frequency estimation. Here we report the Anabaptist Genome Reference Panel (AGRP), the first whole-genome catalogue of variants and phased haplotypes in people of Amish and Mennonite ancestry...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28725850/how-can-home-care-patients-and-their-caregivers-better-manage-fall-risks-by-leveraging-information-technology
#5
Dari Alhuwail, Güneş Koru, Eun-Shim Nahm
OBJECTIVES: From the perspectives of home care patients and caregivers, this study aimed to (a) identify the challenges for better fall-risk management during home care episodes and (b) explore the opportunities for them to leverage health information technology (IT) solutions to improve fall-risk management during home care episodes. METHODS: Twelve in-depth semistructured interviews with the patients and caregivers were conducted within a descriptive single case study design in 1 home health agency (HHA) in the mid-Atlantic region of the United States...
December 2016: Journal of patient experience
https://www.readbyqxmd.com/read/28725789/improving-american-healthcare-through-clinical-lab-2-0-a-project-santa-fe-report
#6
James M Crawford, Khosrow Shotorbani, Gaurav Sharma, Michael Crossey, Tarush Kothari, Thomas S Lorey, Jeffrey W Prichard, Myra Wilkerson, Nancy Fisher
Project Santa Fe was established both to provide thought leadership and to help develop the evidence base for the valuation of clinical laboratory services in the next era of American healthcare. The participants in Project Santa Fe represent major regional health systems that can operationalize laboratory-driven innovations and test their valuation in diverse regional marketplaces in the United States. We provide recommendations from the inaugural March 2016 meeting of Project Santa Fe. Specifically, in the transition from volume-based to value-based health care, clinical laboratories are called upon to provide programmatic leadership in reducing total cost of care through optimization of time-to-diagnosis and time-to-effective therapeutics, optimization of care coordination, and programmatic support of wellness care, screening, and monitoring...
January 2017: Acad Pathol
https://www.readbyqxmd.com/read/28725628/perspectives-on-reflexology-a-qualitative-approach
#7
Nurul Haswani Embong, Yee Chang Soh, Long Chiau Ming, Tin Wui Wong
INTRODUCTION: Reflexology is the systematic practice of applying some pressure to particular points on the feet and hands to impact on health of related parts of the body. OBJECTIVE: To explore the practitioners' perspectives of reflexology in Malaysia. METHODS: Data was collected using face-to-face semi-structured interviews with practitioners in Malaysia. The interviews were conducted in the Malay language and recorded. Interview conversations were translated and transcribed verbatim...
July 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28722827/being-diagnosed-with-cancer-the-experiences-of-patients-with-non-hodgkin-s-lymphoma
#8
Daren Chircop, Josianne Scerri
AIMS AND OBJECTIVES: The aim of this study was to explore the lived experiences of patients on receiving a diagnosis of non-Hodgkin's lymphoma. BACKGROUND: Persons with non-Hodgkin's lymphoma are often diagnosed with cancer when at an advanced stage. This may arise due to a lack of screening programs and delays in help seeking as the symptoms experienced are fairly non-specific in nature. Consequently, receiving an unexpected diagnosis of non-Hodgkin's lymphoma may pose a psychosocial challenge to the individual...
July 19, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28721434/muscle-measures-and-nutritional-status-at-hospital-admission-predict-survival-and-independent-living-of-older-patients-the-empower-study
#9
S Verlaan, J M Van Ancum, V D Pierik, J P Svan Wijngaarden, K Scheerman, C G M Meskers, A B Maier
OBJECTIVES: Older adults with sarcopenia and malnutrition are at risk for co-morbidities, hospitalization, institutionalization, and mortality. In case of hospitalization, risks may be further increased, especially in case of suboptimal dietary intake. The aim of our study was to assess whether muscle mass, muscle strength, functional performance, and nutritional status at hospital admission were associated with survival and independent living among older patients three months after discharge...
2017: Journal of Frailty & Aging
https://www.readbyqxmd.com/read/28720259/empowering-survivors-after-colorectal-and-lung-cancer-treatment-pilot-study-of-a-self-management-survivorship-care-planning-intervention
#10
Anne Reb, Nora Ruel, Marwan Fakih, Lily Lai, Ravi Salgia, Betty Ferrell, Sagus Sampath, Jae Y Kim, Dan J Raz, Virginia Sun
PURPOSE: This study evaluates the feasibility and acceptability of a Self-Management Survivorship Care Planning (SM-SCP) intervention in colorectal and lung cancer survivors. METHODS: This is a single-group, pre- and post-mixed methods study of an advance practice nurse-driven survivorship care intervention that integrates a survivorship care plan with self-management skills coaching. Colorectal and lung cancer survivors with stage I-III disease were enrolled at 3-6 months after completing treatments, and the intervention was administered in one in-person or telephone session...
August 2017: European Journal of Oncology Nursing: the Official Journal of European Oncology Nursing Society
https://www.readbyqxmd.com/read/28720018/structural-risk-and-limits-on-agency-among-exotic-dancers-hiv-risk-practices-in-the-exotic-dance-club
#11
Katherine H A Footer, Sahnah Lim, Merdith R Brantley, Susan G Sherman
This paper provides longitudinal examination of women's health and sexual risk trajectories in US exotic dance clubs, which represent an important commercial setting for the economic mainstreaming of sexual services and an important target for public health programmes. Between July 2014 and May 2015, two semi-structured interviews (at baseline and at three months) were conducted with 24 female exotic dancers who had recently started working in in Baltimore City, USA. Results from a constant comparative analysis point to the interrelationship between the structures of the club setting, including the social context, and women's agentic practices concerning their sexual health...
July 19, 2017: Culture, Health & Sexuality
https://www.readbyqxmd.com/read/28719921/-patient-s-autonomy-and-information-in-psycho-oncology-computer-based-distress-screening-for-an-interactive-treatment-planning-epos-react
#12
Norbert Schäffeler, Jana Sedelmaier, Hannah Möhrer, Katrin Ziser, Johanna Ringwald, Martin Wickert, Sara Brucker, Florian Junne, Stephan Zipfel, Martin Teufel
To identify distressed patients in oncology using screening questionnaires is quite challenging in clinical routine. Up to now there is no evidence based recommendation which instrument is most suitable and how to put a screening to practice. Using computer based screening tools offers the possibility to automatically analyse patient's data, inform psycho-oncological and medical staff about the results, and use reactive questionnaires. Studies on how to empower patients in decision making in psycho-oncology are rare...
July 2017: Psychotherapie, Psychosomatik, Medizinische Psychologie
https://www.readbyqxmd.com/read/28719707/community-triage-of-otology-patients-using-a-store-and-forward-telemedicine-device-a-feasibility-study
#13
Nishi Gupta, Neeraj Chawla, Digant Gupta, Nidhi Dhawan, Vidya R Janaki
Chronic otitis media is a major contributor to acquired hearing loss in developing countries. Developing countries such as India, with huge populations and poor health infrastructures, have always felt the shortage of trained specialists who can provide quality care to meet the enormous demand for treatment of this disease. This pilot study assessed the feasibility of empowering trained health workers equipped with ENTraview, a store-and-forward telemedicine device that integrates a camera- enabled smart phone with an otoscope...
July 2017: Ear, Nose, & Throat Journal
https://www.readbyqxmd.com/read/28719415/heart-failure-self-care-to-success-development-and-evaluation-of-a-program-toolkit
#14
Rebecca Bryant
The Heart Failure: Self-care to Success toolkit was developed to assist NPs in empowering patients with heart failure (HF) to improve individual self-care behaviors. This article details the evolution of this toolkit for NPs, its effectiveness with patients with HF, and recommendations for future research and dissemination strategies.
August 17, 2017: Nurse Practitioner
https://www.readbyqxmd.com/read/28719144/maximizing-the-stability-of-metabolic-engineering-derived-whole-cell-biocatalysts
#15
REVIEW
Marvin Kadisch, Christian Willrodt, Michael Hillen, Bruno Bühler, Andreas Schmid
A systematic and powerful knowledge-based framework exists for improving the activity and stability of chemical catalysts and for empowering the commercialization of respective processes. In contrast, corresponding biotechnological processes are still scarce and characterized by case-by-case development strategies. A systematic understanding of parameters affecting biocatalyst efficiency, that is, biocatalyst activity and stability, is essential for a rational generation of improved biocatalysts. Today, systematic approaches only exist for increasing the activity of whole-cell biocatalysts...
July 18, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28718928/accessing-support-and-empowerment-online-the-experiences-of-individuals-with-diabetes
#16
Ellen Brady, Julia Segar, Caroline Sanders
CONTEXT: The use of the internet for health information by those with long-term conditions is growing. It has been argued that this represents a form of empowerment by patients, as it enables them to control the content and flow of the information available to them. To explore this, the use of online discussion groups by those with diabetes was examined. METHOD: Semi-structured interviews were conducted with 21 participants with type 1 and 2 diabetes and analysed using thematic analysis...
July 18, 2017: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/28718079/symptom-self-management-strategies-reported-by-adolescents-and-young-adults-with-cancer-receiving-chemotherapy
#17
Lauri A Linder, Jeanne M Erickson, Kristin Stegenga, Catherine Fiona Macpherson, Sarah Wawrzynski, Christina Wilson, Suzanne Ameringer
PURPOSE: Adolescents and young adults (AYAs) with cancer experience multiple symptoms related to their cancer and its treatment which can negatively impact their development and quality of life. An understanding of the strategies AYAs use to self-manage their symptoms is limited. This study described symptom self-management strategies reported by AYAs with cancer using an iPad-based symptom heuristics tool, the Computerized Symptom Capture Tool. METHODS: The study used a cross-sectional, descriptive design...
July 17, 2017: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/28717713/breaking-cancer-bad-news-to-patients-with-cancer-a-comprehensive-perspective-of-patients-their-relatives-and-the-public-example-from-a-middle-eastern-country
#18
Jamal Zekri, Syed Mustafa Karim
PURPOSE: Empowering patients with cancer requires that they be continuously informed about their condition. In some Eastern cultures, this concept is often opposed by caregivers. We aim to compare the extent of disclosure desired by actual and presumed patients with cancer and their relatives in our practice. METHODS: Nine questions reflecting possible bad news communication to patients from diagnosis to the end of life were designed to investigate the extent of desired disclosure and were answered by 100 patients (cohort I) and 103 accompanying relatives (cohort II) in an outpatient setting...
October 2016: Journal of Global Oncology
https://www.readbyqxmd.com/read/28716980/early-dysphagia-screening-by-trained-nurses-reduces-pneumonia-rate-in-stroke-patients-a-clinical-intervention-study
#19
Christoph Palli, Simon Fandler, Kathrin Doppelhofer, Kurt Niederkorn, Christian Enzinger, Christian Vetta, Esther Trampusch, Reinhold Schmidt, Franz Fazekas, Thomas Gattringer
BACKGROUND AND PURPOSE: Dysphagia is a common stroke symptom and leads to serious complications such as aspiration and pneumonia. Early dysphagia screening can reduce these complications. In many hospitals, dysphagia screening is performed by speech-language therapists who are often not available on weekends/holidays, which results in delayed dysphagia assessment. METHODS: We trained the nurses of our neurological department to perform formal dysphagia screening in every acute stroke patient by using the Gugging Swallowing Screen...
July 17, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28714833/patients-expectations-and-solutions-for-improving-primary-diabetes-care
#20
Brigitte Vachon, Ai-Thuy Huynh, Mylaine Breton, Louise Quesnel, Michel Camirand, Jeannette Leblanc, Sylvie Tardif
Purpose The purpose of this paper is to document health care needs expressed by people living with diabetes, describe the solutions they envisaged for improving the quality of primary care (PC) services and empower them to make better use of PC services. Design/methodology/approach A participatory research approach was used. Six workshops were organised to provide diabetes patients with knowledge on available services and to engage them in sharing their experience. Group discussions were recorded. Data were analysed using the thematic analysis method...
July 10, 2017: International Journal of Health Care Quality Assurance
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