keyword
MENU ▼
Read by QxMD icon Read
search

Plant Flux analysis

keyword
https://www.readbyqxmd.com/read/28642707/tetrandrine-an-activator-of-autophagy-induces-autophagic-cell-death-via-pkc-%C3%AE-inhibition-and-mtor-dependent-mechanisms
#1
Vincent Kam Wai Wong, Wu Zeng, Juan Chen, Xiao Jun Yao, Elaine Lai Han Leung, Qian Qian Wang, Pauline Chiu, Ben C B Ko, Betty Yuen Kwan Law
Emerging evidence suggests the therapeutic role of autophagic modulators in cancer therapy. This study aims to identify novel traditional Chinese medicinal herbs as potential anti-tumor agents through autophagic induction, which finally lead to autophagy mediated-cell death in apoptosis-resistant cancer cells. Using bioactivity-guided purification, we identified tetrandrine (Tet) from herbal plant, Radix stephaniae tetrandrae, as an inducer of autophagy. Across a number of cancer cell lines, we found that breast cancer cells treated with tetrandrine show an increase autophagic flux and formation of autophagosomes...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28629120/metabolomics-characterization-of-two-apocynaceae-plants-catharanthus-roseus-and-vinca-minor-using-gc-ms-and-lc-ms-methods-in-combination
#2
Qi Chen, Xueyan Lu, Xiaorui Guo, Qingxi Guo, Dewen Li
Catharanthus roseus (C. roseus) and Vinca minor (V. minor) are two common important medical plants belonging to the family Apocynaceae. In this study, we used non-targeted GC-MS and targeted LC-MS metabolomics to dissect the metabolic profile of two plants with comparable phenotypic and metabolic differences. A total of 58 significantly different metabolites were present in different quantities according to PCA and PLS-DA score plots of the GC-MS analysis. The 58 identified compounds comprised 16 sugars, eight amino acids, nine alcohols and 18 organic acids...
June 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28625541/plant-derived-antifungal-compounds-trigger-a-common-transcriptional-response
#3
Zhe Wang
Understanding the mechanism of action of antifungal drugs is vital for better control of mycosis, which kills >1.3 million lives every year thus remains a major health problem worldwide. In this study, we investigate the activities of three different categories of plant-derived antifungal compounds (resveratrol, honokiol and osthole) via transcriptomics and bioinformatics analysis, with the goal of discovering the common Mode-of-Action (MoA) at molecular level. The result shows that a common transcriptional response (72 gene are up-regulated while 10 genes are down-regulated, commonly) are triggered by above representative antifungal compounds in Schizosaccharomyces pombe (S...
June 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28614594/linkages-of-plant-stoichiometry-to-ecosystem-production-and-carbon-fluxes-with-increasing-nitrogen-inputs-in-an-alpine-steppe
#4
Yunfeng Peng, Fei Li, Guoying Zhou, Kai Fang, Dianye Zhang, Changbin Li, Guibiao Yang, Guanqin Wang, Jun Wang, Yuanhe Yang
Unprecedented levels of nitrogen (N) have entered terrestrial ecosystems over the past century, which substantially influences the carbon (C) exchange between the atmosphere and biosphere. Temperature and moisture are generally regarded as the major controllers over the N effects on ecosystem C uptake and release. N-phosphorous (P) stoichiometry regulates the growth and metabolisms of plants and soil organisms, thereby affecting many ecosystem C processes. However, it remains unclear how the N-induced shift in the plant N:P ratio affects ecosystem production and C fluxes and its relative importance...
June 14, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28605605/nanosensor-technology-applied-to-living-plant-systems
#5
Seon-Yeong Kwak, Min Hao Wong, Tedrick Thomas Salim Lew, Gili Bisker, Michael A Lee, Amir Kaplan, Juyao Dong, Albert Tianxiang Liu, Volodymyr B Koman, Rosalie Sinclair, Catherine Hamann, Michael S Strano
An understanding of plant biology is essential to solving many long-standing global challenges, including sustainable and secure food production and the generation of renewable fuel sources. Nanosensor platforms, sensors with a characteristic dimension that is nanometer in scale, have emerged as important tools for monitoring plant signaling pathways and metabolism that are nondestructive, minimally invasive, and capable of real-time analysis. This review outlines the recent advances in nanotechnology that enable these platforms, including the measurement of chemical fluxes even at the single-molecule level...
June 12, 2017: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/28596620/respiratory-oxygen-consumption-in-the-seagrass-zostera-marina-varies-on-a-diel-basis-and-is-partly-affected-by-light
#6
Lina M Rasmusson, Chiara Lauritano, Gabriele Procaccini, Martin Gullström, Pimchanok Buapet, Mats Björk
The seagrass Zostera marina is an important marine ecosystem engineer, greatly influencing oxygen and carbon fluctuations in temperate coastal areas. Although photosynthetically driven gas fluxes are well studied, the impact of the plant's mitochondrial respiration on overall CO2 and O2 fluxes in marine vegetated areas is not yet understood. Likewise, the gene expression in relation to the respiratory pathway has not been well analyzed in seagrasses. This study uses a combined approach, studying respiratory oxygen consumption rates in darkness simultaneously with changes in gene expression, with the aim of examining how respiratory oxygen consumption fluctuates on a diel basis...
2017: Marine Biology
https://www.readbyqxmd.com/read/28596613/effect-of-climate-warming-on-the-annual-terrestrial-net-ecosystem-co2-exchange-globally-in-the-boreal-and-temperate-regions
#7
Zhiyuan Zhang, Renduo Zhang, Alessandro Cescatti, Georg Wohlfahrt, Nina Buchmann, Juan Zhu, Guanhong Chen, Fernando Moyano, Jukka Pumpanen, Takashi Hirano, Kentaro Takagi, Lutz Merbold
The net ecosystem CO2 exchange is the result of the imbalance between the assimilation process (gross primary production, GPP) and ecosystem respiration (RE). The aim of this study was to investigate temperature sensitivities of these processes and the effect of climate warming on the annual terrestrial net ecosystem CO2 exchange globally in the boreal and temperate regions. A database of 403 site-years of ecosystem flux data at 101 sites in the world was collected and analyzed. Temperature sensitivities of rates of RE and GPP were quantified with Q 10, defined as the increase of RE (or GPP) rates with a temperature rise of 10 °C...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28559925/integrated-analysis-of-454-and-illumina-transcriptomic-sequencing-characterizes-carbon-flux-and-energy-source-for-fatty-acid-synthesis-in-developing-lindera-glauca-fruits-for-woody-biodiesel
#8
Zixin Lin, Jiyong An, Jia Wang, Jun Niu, Chao Ma, Libing Wang, Guanshen Yuan, Lingling Shi, Lili Liu, Jinsong Zhang, Zhixiang Zhang, Ji Qi, Shanzhi Lin
BACKGROUND: Lindera glauca fruit with high quality and quantity of oil has emerged as a novel potential source of biodiesel in China, but the molecular regulatory mechanism of carbon flux and energy source for oil biosynthesis in developing fruits is still unknown. To better develop fruit oils of L. glauca as woody biodiesel, a combination of two different sequencing platforms (454 and Illumina) and qRT-PCR analysis was used to define a minimal reference transcriptome of developing L...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28554116/riparian-vegetation-in-the-alpine-connectome-terrestrial-aquatic-and-terrestrial-terrestrial-interactions
#9
Dragos G Zaharescu, Antonio Palanca-Soler, Peter S Hooda, Catalin Tanase, Carmen I Burghelea, Richard N Lester
Alpine regions are under increased attention worldwide for their critical role in early biogeochemical cycles, their high sensitivity to environmental change, and as repositories of natural resources of high quality. Their riparian ecosystems, at the interface between aquatic and terrestrial environments, play important geochemical functions in the watershed and are biodiversity hotspots, despite a harsh climate and topographic setting. With climate change rapidly affecting the alpine biome, we still lack a comprehensive understanding of the extent of interactions between riparian surface, lake and catchment environments...
May 26, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28546437/characterization-of-thermostable-cam-rubisco-activase-reveals-a-rubisco-interacting-surface-loop
#10
Devendra Shivhare, Oliver Mueller-Cajar
In order to maintain metabolic flux through the Calvin-Benson-Bassham (CBB) cycle in higher plants, dead-end inhibited complexes of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) must constantly be engaged and remodeled by the molecular chaperone Rubisco activase (Rca). In C3 plants the thermolability of Rca is responsible for the deactivation of Rubisco and reduction of photosynthesis at moderately elevated temperatures. We reasoned that CAM plants must possess thermostable Rca to support CBB flux during the day when stomata are closed...
May 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28527413/monochromatic-green-light-induces-an-aberrant-accumulation-of-geranylgeranyled-chlorophylls-in-plants
#11
Zuzana Materová, Roman Sobotka, Barbora Zdvihalová, Michal Oravec, Jakub Nezval, Václav Karlický, Daniel Vrábl, Michal Štroch, Vladimír Špunda
Light quality is an important environmental factor affecting the biosynthesis of photosynthetic pigments whose production seems to be affected not only quantitatively but also qualitatively. In this work, we set out to identify unusual pigment detected in leaves of barley (Hordeum vulgare L.) and explain its presence in plants grown under monochromatic green light (GL; 500-590 nm). The chromatographic analysis (HPLC-DAD) revealed that a peak belonging to this unknown pigment is eluted between chlorophyll (Chl) a and b...
May 6, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28526789/a-novel-iron-dependent-enzyme-that-catalyzes-the-initial-step-in-the-biodegradation-of-n-nitroglycine-by-variovorax-sp-strain-js1663
#12
Kristina M Mahan, Hangping Zheng, Tekle T Fida, Ronald J Parry, David E Graham, Jim C Spain
Nitramines are key constituents of most of the explosives in current use and consequently contaminate soil and groundwater at many military facilities around the world. Toxicity from nitramine contamination poses a health risk to plants and animals, thus understanding how nitramines are biodegraded is critical to environmental remediation. The biodegradation of synthetic nitramine compounds such as hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX) has been studied for decades, but little is known about the catabolism of naturally produced nitramine compounds...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28520776/proteomic-analysis-of-tea-plants-camellia-sinensis-with-purple-young-shoots-during-leaf-development
#13
Qiongqiong Zhou, Zhidan Chen, Jinwook Lee, Xinghui Li, Weijiang Sun
Tea products made from purple leaves are highly preferred by consumers due to the health benefits. This study developed a proteome reference map related to color changes during leaf growth in tea (Camellia sinensis) plant with purple young shoots using two-dimensional electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel and successfully identified by using MALDI-TOF/TOF-MS. The pronounced changes in the proteomic profile between tender purple leaves (TPL) and mature green leaves (MGL) included: 1) the lower activity of proteins associated with CO2 assimilation, energy metabolism and photo flux efficiency and higher content of anthocyanins in TPL than those in MGL may protect tender leaves against photo-damage; 2) the higher abundance of chalcone synthase (CHS), chalcone isomerase (CHI) and flavonol synthase (FLS) likely contributes to the synthesis of anthocyanins, catechins and flavonols in TPL tissues; 3) higher abundance of stress response proteins, such as glutathione S-transferases (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx), could enhance the tolerance of TPL tissues to adverse condition in; and 4) the increased abundance of proteins related to protein synthesis, nucleic acids and cell wall proteins should be beneficial for the proliferation and expansion of leaf cell in TPL tissues...
2017: PloS One
https://www.readbyqxmd.com/read/28515881/effect-of-bursaphelenchus-xylophilus-infection-on-leaf-photosynthetic-characteristics-and-resource-use-efficiency-of-pinus-massoniana
#14
Ruihe Gao, Zhuang Wang, Juan Shi, Youqing Luo
Pine wilt disease (PWD) is considered as the most destructive forest-invasive alien species in China. We measured gas exchange parameters and foliar carbon isotope ratios (δ(13)C) of different infection phases of Masson pine in order to investigate the effect of Bursaphelenchus xylophilus infection on photosynthetic responses and resource-use efficiency. The results showed that net photosynthetic rate (Pn), transpiration rate (T), stomatal conductance (gs), and internal CO 2 concentrations (Ci) decreased in the infested trees at photosynthetic photon flux density (PPFD) levels from 0 to 2,000 μmol m(-2) s(-1) compared with controls...
May 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28505579/effect-of-lupinus-albus-l-root-activities-on-as-and-cu-mobility-after-addition-of-iron-based-soil-amendments
#15
Teresa Fresno, Jesús M Peñalosa, Jakob Santner, Markus Puschenreiter, Eduardo Moreno-Jiménez
Arsenic and Cu mobility was investigated in the rhizosphere of Lupinus albus L. grown in an iron-amended contaminated soil. White lupin was grown in rhizobags in contaminated soil either left untreated or amended with iron sulphate plus lime (Fe + lime) or biochar (Fe + BC). Porewater was monitored in rhizosphere and bulk soil throughout the experiment and the extractable fraction of several elements and As and Cu plant uptake was analysed after 48 days. The distribution of As, Cu, P and Fe in the lupin rhizosphere was evaluated with chemical images obtained by laser ablation-ICP-MS analysis of diffusive gradients in thin films (DGT) gels...
May 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28480159/tracing-insights-into-human-metabolism-using-chemical-engineering-approaches
#16
Thekla Cordes, Christian M Metallo
Metabolism coordinates the conversion of available nutrients toward energy, biosynthetic intermediates, and signaling molecules to mediate virtually all biological functions. Dysregulation of metabolic pathways contributes to many diseases, so a detailed understanding of human metabolism has significant therapeutic implications. Over the last decade major technological advances in the areas of analytical chemistry, computational estimation of intracellular fluxes, and biological engineering have improved our ability to observe and engineer metabolic pathways...
November 2016: Current Opinion in Chemical Engineering
https://www.readbyqxmd.com/read/28469419/an-insight-into-structure-function-and-expression-analysis-of-3-hydroxy-3-methylglutaryl-coa-reductase-of-cymbopogon-winterianus
#17
Kamalakshi Devi, Lochana Patar, Mahendra K Modi, Priyabrata Sen
Citronella (Cymbopogon winterianus) is one of the richest sources of high-value isoprenoid aromatic compounds used as flavour, fragrance, and therapeutic elements. These isoprenoid compounds are synthesized by 2 independent pathways: mevalonate pathway and 2-C-methyl-d-erythritol-4-phosphate pathway. Evidence suggests that 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a rate-controlling enzyme for the synthesis of variety of isoprenoids. This study reports the isolation, characterization, and tissue-specific expression analysis of HMGR from citronella...
2017: Bioinformatics and Biology Insights
https://www.readbyqxmd.com/read/28458109/sulfur-flows-and-biosolids-processing-using-material-flux-analysis-mfa-principles-at-wastewater-treatment-plants
#18
R M Fisher, J P Alvarez-Gaitan, R M Stuetz, S J Moore
High flows of sulfur through wastewater treatment plants (WWTPs) may cause noxious gaseous emissions, corrosion of infrastructure, inhibit wastewater microbial communities, or contribute to acid rain if the biosolids or biogas is combusted. Yet, sulfur is an important agricultural nutrient and the direct application of biosolids to soils enables its beneficial re-use. Flows of sulfur throughout the biosolids processing of six WWTPs were investigated to identify how they were affected by biosolids processing configurations...
April 27, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28456110/quantitative-assessment-of-energy-and-resource-recovery-in-wastewater-treatment-plants-based-on-plant-wide-simulations
#19
T Fernández-Arévalo, I Lizarralde, F Fdz-Polanco, S I Pérez-Elvira, J M Garrido, S Puig, M Poch, P Grau, E Ayesa
The growing development of technologies and processes for resource treatment and recovery is offering endless possibilities for creating new plant-wide configurations or modifying existing ones. However, the configurations' complexity, the interrelation between technologies and the influent characteristics turn decision-making into a complex or unobvious process. In this frame, the Plant-Wide Modelling (PWM) library presented in this paper allows a thorough, comprehensive and refined analysis of different plant configurations that are basic aspects in decision-making from an energy and resource recovery perspective...
July 1, 2017: Water Research
https://www.readbyqxmd.com/read/28444347/fluxomics-links-cellular-functional-analyses-to-whole-plant-phenotyping
#20
Christophe Salon, Jean-Christophe Avice, Sophie Colombié, Martine Dieuaide-Noubhani, Karine Gallardo, Christian Jeudy, Alain Ourry, Marion Prudent, Anne-Sophie Voisin, Dominique Rolin
Fluxes through metabolic pathways reflect the integration of genetic and metabolic regulations. While it is attractive to measure all the mRNAs (transcriptome), all the proteins (proteome), and a large number of the metabolites (metabolome) in a given cellular system, linking and integrating this information remains difficult. Measurement of metabolome-wide fluxes (termed the fluxome) provides an integrated functional output of the cell machinery and a better tool to link functional analyses to plant phenotyping...
April 1, 2017: Journal of Experimental Botany
keyword
keyword
65001
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"