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https://www.readbyqxmd.com/read/27825488/trichoderma-asperellum-spore-dose-depended-modulation-of-plant-growth-in-vegetable-crops
#1
Vivek Singh, Ram Sanmukh Upadhyay, Birinchi Kumar Sarma, Harikesh Bahadur Singh
In the present study we bioprimed seeds of six vegetable crops (tomato, brinjal, chilli, okra, ridge gourd and guar) with different spore doses of Trichoderma asperellum BHUT8 ranging from 10(2) to 10(8) spores ml(-1) and the effect of biopriming was seen on seed germination and development. The most effective spore dose for enhancement in seed germination and radicle length was found to be 10(3) spores ml(-1) in tomato and ridge gourd, 10(4) spores ml(-1) in brinjal and okra while 10(6) spores ml(-1) in chilli and guar...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27585825/mitochondrial-trafficking-through-rhot1-is-involved-in-the-aggregation-of-germinal-granule-components-during-primordial-germ-cell-formation-in-xenopus-embryos
#2
Haru Tada, Yuya Taira, Keisuke Morichika, Tsutomu Kinoshita
In many animals, the germ plasm is sufficient and necessary for primordial germ cell (PGC) formation. It contains germinal granules and abundant mitochondria (germline-Mt). However, the role of germline-Mt in germ cell formation remains poorly understood. In Xenopus, the germ plasm is distributed as many small islands at the vegetal pole, which gradually aggregates to form a single large mass in each of the four vegetal pole cells at the early blastula stage. Polymerized microtubules and the adapter protein kinesin are required for the aggregation of germ plasm...
October 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27092118/nanocalorimetric-characterization-of-microbial-activity-in-deep-subsurface-oceanic-crustal-fluids
#3
Alberto Robador, Douglas E LaRowe, Sean P Jungbluth, Huei-Ting Lin, Michael S Rappé, Kenneth H Nealson, Jan P Amend
Although fluids within the upper oceanic basaltic crust harbor a substantial fraction of the total prokaryotic cells on Earth, the energy needs of this microbial population are unknown. In this study, a nanocalorimeter (sensitivity down to 1.2 nW ml(-1)) was used to measure the enthalpy of microbially catalyzed reactions as a function of temperature in samples from two distinct crustal fluid aquifers. Microorganisms in unamended, warm (63°C) and geochemically altered anoxic fluids taken from 292 meters sub-basement (msb) near the Juan de Fuca Ridge produced 267...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26919813/optimization-of-heat-and-relative-humidity-conditions-to-reduce-escherichia-coli-o157-h7-contamination-and-maximize-the-germination-of-radish-seeds
#4
M K Song, H W Kim, M S Rhee
We previously reported that a combination of heat and relative humidity (RH) had a marked bactericidal effect on Escherichia coli O157:H7 on radish seeds. Here, response surface methodology with a Box-Behnken design was used to build a model to predict reductions in E. coli O157:H7 populations based on three independent variables: heating temperature (55 °C, 60 °C, or 65 °C), RH (40%, 60%, and 80%), and holding time (8, 15, or 22 h). Optimum treatment conditions were selected using a desirability function...
June 2016: Food Microbiology
https://www.readbyqxmd.com/read/26494182/very-small-embryonic-like-stem-cells-a-potential-developmental-link-between-germinal-lineage-and-hematopoiesis-in-humans
#5
REVIEW
Irma Virant-Klun
It has been suggested that hematopoietic stem/progenitor cells (HSPCs) could become specified from a population of migrating primordial germ cells (PGCs), precursors of gametes, during embryogenesis. Some recent experimental data demonstrated that the cell population that is usually considered to be PGCs, moving toward the gonadal ridges of an embryo, contains a subset of cells coexpressing several germ cell and hematopoietic markers and possessing hematopoietic activity. Experimental data showed that bone morphogenetic protein 4 (BMP4) generates PGCs from mouse bone marrow-derived pluripotent stem cells...
January 15, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/26037665/in-vitro-generation-and-characterization-of-chicken-long-term-germ-cells-from-different-embryonic-origins
#6
Franca Raucci, Aurelie Fuet, Bertrand Pain
Primordial germ cells (PGCs) are the precursors of differentiated germ cells. Located in the epiblast of a stage X (EG&K) embryo, the PGCs translocate anteriorly to the germinal crescent and migrate, within 48 to 56 hours of development, through the blood vascular system to the germinal ridges where they become the gonadal germ cells (GGCs). We aim to generate, compare, and determine the basic characters of the in vitro long-term cultured PGCs derived from (1) the chicken blastodermal cells (at stages IX-XII); (2) the chicken blood of a 2-day old embryo (stages 14-17 Hamburger Hamilton [HH]); and (3) the long-term cultured gonocytes taken from male gonads of a 5- to 6-day-old embryo (stages 29-30 HH)...
September 15, 2015: Theriogenology
https://www.readbyqxmd.com/read/25707852/virus-induced-gene-silencing-based-functional-verification-of-six-genes-associated-with-vernalization-in-wheat
#7
Ya-Lan Feng, Ke-Tao Wang, Chao Ma, Yong-Ying Zhao, Jun Yin
Vernalization requirement is an important characteristic in crop breeding. Wheat is a widely grown crop in the world that possesses enormous economic significance. To better understand the gene networks in vernalization process, we performed a high-throughput RNA sequencing analysis comparing the transcriptomes of spring and winter wheat cultivars, with and without vernalization (unpublished data). In this study, we selected six unigenes (CL14010, CL12788, CL176, Unigene 16777, CL8746 and Unigene10196) from our transcriptome analysis based on their expression differences to further characterize their function...
March 20, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25424782/portrait-of-dr-michael-g-hanna-jr-a-pioneer-in-active-specific-immunotherapy-of-cancer
#8
Michael Hanna
While a PhD candidate, doing my thesis at the Oak Ridge National Laboratory, Biology Division under Dr. Charles Congdon, my introduction to the immune response was studying graft vs. host (GVH) disease as a consequence of bone marrow transplantation in mice. The sequalae of GVH was impressive, and demonstrated the potential of negative clinical consequences of the immune system. The idea of harnessing this immunological phenomena in cancer therapy was appealing even in the late 1960s. The problem was that at the time T-cells as a component of the immune system were identified but not defined...
2014: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/25424781/human-vaccines-immunotherapeutics-news
#9
Michael Hanna
While a PhD candidate, doing my thesis at the Oak Ridge National Laboratory, Biology Division under Dr. Charles Congdon, my introduction to the immune response was studying graft vs. host (GVH) disease as a consequence of bone marrow transplantation in mice. The sequalae of GVH was impressive, and demonstrated the potential of negative clinical consequences of the immune system. The idea of harnessing this immunological phenomena in cancer therapy was appealing even in the late 1960s. The problem was that at the time T-cells as a component of the immune system were identified but not defined...
2014: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/25165118/generation-of-mouse-functional-oocytes-in-rat-by-xeno-ectopic-transplantation-of-primordial-germ-cells
#10
Tomonari Hayama, Tomoyuki Yamaguchi, Megumi Kato-Itoh, Sanae Hamanaka, Mami Kawarai, Makoto Sanbo, Chihiro Tamura, Youn-Su Lee, Ayaka Yanagida, Hideyuki Murayama, Naoaki Mizuno, Ayumi Umino, Hideyuki Sato, Satoshi Yamazaki, Hideki Masaki, Toshihiro Kobayashi, Masumi Hirabayashi, Hiromitsu Nakauchi
Primordial germ cells (PGCs) are germ cell progenitors in the fetal genital ridge; female PGCs give rise to definitive oocytes that contribute to the next generation. Artificial PGCs have been induced in vitro from pluripotent stem cells and gonad-like tissue has been induced in vivo by cotransplantation of PGCs with PGC-free gonadal cells. To apply these technologies to human infertility treatment or conservation of rare species, PGC transplantation must be established in xenogenic animals. Here, we established a xenogeneic transplantation model by inducing ovary-like tissue from PGCs in xenogenic animals...
October 2014: Biology of Reproduction
https://www.readbyqxmd.com/read/25123578/differentiation-and-morphogenesis-of-the-ovary-and-expression-of-gonadal-development-related-genes-in-the-protogynous-hermaphroditic-ricefield-eel-monopterus-albus
#11
Z He, Y Li, Y Wu, S Shi, C Sun, Q Deng, J Xie, T Wang, W Zhang, L Zhang
The ovarian differentiation, morphogenesis and expression of some putative gonadal development-related genes were analysed in the ricefield eel Monopterus albus, a protogynous hermaphroditic teleost with a single elongate ovary. At c. 1 day post-hatching (dph), the gonadal ridge was colonized with primordial germ cells (PGCs) at the periphery and transformed into the gonadal primordium, which appeared to contain two germinal epithelia. At c. 7 dph, four ovarian cavities appeared in the gonadal tissue with two in each germinal epithelial compartment, and the indifferent gonad might have begun to differentiate into the ovary...
November 2014: Journal of Fish Biology
https://www.readbyqxmd.com/read/25045900/portrait
#12
Michael Hanna
While a PhD candidate, doing my thesis at the Oak Ridge National Laboratory, Biology Division under Dr. Charles Congdon, my introduction to the immune response was studying graft vs. host (GVH) disease as a consequence of bone marrow transplantation in mice. The sequalae of GVH was impressive, and demonstrated the potential of negative clinical consequences of the immune system. The idea of harnessing this immunological phenomena in cancer therapy was appealing even in the late 1960s. The problem was that at the time T-cells as a component of the immune system were identified but not defined...
July 21, 2014: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/24697411/primordial-germ-cells-in-the-dorsal-mesentery-of-the-chicken-embryo-demonstrate-left-right-asymmetry-and-polarized-distribution-of-the-ema1-epitope
#13
Gideon Hen, Miriam Friedman-Einat, Dalit Sela-Donenfeld
Despite the importance of the chicken as a model system, our understanding of the development of chicken primordial germ cells (PGCs) is far from complete. Here we characterized the morphology of PGCs at different developmental stages, their migration pattern in the dorsal mesentery of the chicken embryo, and the distribution of the EMA1 epitope on PGCs. The spatial distribution of PGCs during their migration was characterized by immunofluorescence on whole-mounted chicken embryos and on paraffin sections, using EMA1 and chicken vasa homolog antibodies...
May 2014: Journal of Anatomy
https://www.readbyqxmd.com/read/24513561/an-algorithmic-approach-to-posttraumatic-nail-deformities-based-on-anatomical-classification
#14
Ashish Rai, Manoj K Jha, Lalit K Makhija, Sameek Bhattacharya, Nitin Sethi, Shilpi Baranwal
BACKGROUND AND AIMS: Most of the clinical series on posttraumatic nail deformities (PTNDs) address an individual deformity and its correction. The aim of the study was to classify PTND on the basis of its anatomical defect, devise the reconstructive modality and propose an algorithmic approach to PTND. We have also analysed our results of surgical correction and compared the data with the published literature. METHOD: A 5-year retrospective study of 45 patients with PTND was conducted...
April 2014: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/23894634/the-influence-of-variable-rainfall-frequency-on-germination-and-early-growth-of-shade-tolerant-dipterocarp-seedlings-in-borneo
#15
Michael J O'Brien, Christopher D Philipson, John Tay, Andy Hector
Climate change induced alterations to rainfall patterns have the potential to affect the regeneration dynamics of plant species, especially in historically everwet tropical rainforest. Differential species response to infrequent rainfall may influence seed germination and seedling establishment in turn affecting species distributions. We tested the role of watering frequency intervals (from daily to six-day watering) on the germination and the early growth of Dipterocarpaceae seedlings in Borneo. We used seeds that ranged in size from 500 to 20,000 mg in order to test the role of seed mass in mediating the effects of infrequent watering...
2013: PloS One
https://www.readbyqxmd.com/read/23741212/eczematous-dermatitis-occurring-on-a-caf%C3%A3-au-lait-spot-long-after-laser-radiation
#16
Motoyuki Mihara
A 40-year-old woman presented with an itchy erythematosquamous change of a café-au-lait spot in her face. The onset of this change occurred just after her relocation. The café-au-lait spot had been irradiated by laser approximately 20 years ago. Clinically, there was a coin-sized erythema with a slight scale on the pigmented lesion in the left lateral orbital region. Histopathologically, the lesion demonstrated both spongiotic dermatitis and interface dermatitis together with lymphohistiocytic cell infiltration, in addition to moderate acanthosis and elongation of rete ridges with slight basal hyperpigmentation...
May 2013: Case Reports in Dermatology
https://www.readbyqxmd.com/read/23685333/tbx18-expression-demarcates-multipotent-precursor-populations-in-the-developing-urogenital-system-but-is-exclusively-required-within-the-ureteric-mesenchymal-lineage-to-suppress-a-renal-stromal-fate
#17
Tobias Bohnenpoll, Eva Bettenhausen, Anna-Carina Weiss, Anna B Foik, Mark-Oliver Trowe, Patrick Blank, Rannar Airik, Andreas Kispert
The mammalian urogenital system derives from multipotent progenitor cells of different germinal tissues. The contribution of individual sub-populations to specific components of the mature system, and the spatiotemporal restriction of the respective lineages have remained poorly characterized. Here, we use comparative expression analysis to delineate sub-regions within the developing urogenital system that express the T-box transcription factor gene Tbx18. We show that Tbx18 is transiently expressed in the epithelial lining and the subjacent mesenchyme of the urogenital ridge...
August 1, 2013: Developmental Biology
https://www.readbyqxmd.com/read/22991581/development-of-the-germinal-ridge-and-ovary-in-the-african-elephant-loxodonta-africana
#18
F J Stansfield, J O Nöthling, J T Soley, W R Allen
The follicular reserve and its ontogeny in the elephant are of interest because elephants have the longest reproductive life of all land-based mammals. They also have the longest recorded pregnancy, which allows a protracted view of the series of significant events involved in the development of the embryonic and fetal gonads. The large elephant population of Zimbabwe provided the opportunity to collect conceptuses from elephants culled for management reasons and hunted professionally. Five embryos aged 76-96 days and the ovaries of four fetuses aged 4...
November 2012: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/22551673/disinfection-of-wheat-grains-contaminated-with-penicillium-oxalicum-spores-by-a-supercritical-carbon-dioxide-water-cosolvent-system
#19
Hyong Seok Park, Yong Ho Lee, Wook Kim, Hee Jung Choi, Kyoung Heon Kim
Spores of the plant pathogenic fungus Penicillium oxalicum inoculated onto wheat grains were inactivated using supercritical carbon dioxide (SC-CO(2)). After the SC-CO(2) treatment at various conditions of temperature, time and amount of water cosolvent, the colony forming units (CFU) of fungal spores on wheat grains and the germination yields of wheat grains were determined. Among these SC-CO(2) treatment parameters, the inactivation of P. oxalicum spores was found to be significantly increased by adding water cosolvent...
June 1, 2012: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/22539516/edaphic-adaptation-maintains-the-coexistence-of-two-cryptic-species-on-serpentine-soils
#20
Jenn M Yost, Teri Barry, Kathleen M Kay, Nishanta Rajakaruna
PREMISE OF THE STUDY: Divergent edaphic adaptation can contribute to reproductive isolation and coexistence between closely related species, yet we know little about how small-scale continuous edaphic gradients contribute to this phenomenon. We investigated edaphic adaptation between two cryptic species of California wildflower, Lasthenia californica and L. gracilis (Asteraceae), which grow in close parapatry on serpentine soil. METHODS: We reciprocally transplanted both species into the center of each species' habitat and the transition zone between species...
May 2012: American Journal of Botany
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