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Blood biomarker proteomic

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https://www.readbyqxmd.com/read/28196416/blood-proteomic-profiling-in-inherited-attrm-and-acquired-attrwt-forms-of-transthyretin-associated-cardiac-amyloidosis
#1
Gloria G Chan, Clarissa M Koch, Lawreen H Connors
Transthyretin-associated forms of cardiac amyloidosis are fatal protein misfolding diseases that can be inherited (ATTRm) or acquired (ATTRwt). Precise biomarkers for ATTR disease identification and monitoring are undiscovered, disease-specific therapeutic options are needed, and the current understanding of ATTR molecular pathogenesis is limited. The aim of this study was to investigate and compare the serum proteomes in ATTRm and ATTRwt cardiac amyloidosis to identify differentially expressed blood proteins that were disease-specific...
February 14, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28186866/toward-postgenomics-ophthalmology-a-proteomic-map-of-the-human-choroid-retinal-pigment-epithelium-tissue
#2
Manjunath Dammalli, Krishna R Murthy, Sneha M Pinto, Kalpana Babu Murthy, Raja Sekhar Nirujogi, Anil K Madugundu, Gourav Dey, Bipin Nair, Harsha Gowda, Thottethodi Subrahmanya Keshava Prasad
Ophthalmology and visual health research have received relatively limited attention from the personalized medicine community, but this trend is rapidly changing. Postgenomics technologies such as proteomics are being utilized to establish a baseline biological variation map of the human eye and related tissues. In this context, the choroid is the vascular layer situated between the outer sclera and the inner retina. The choroidal circulation serves the photoreceptors and retinal pigment epithelium (RPE). The RPE is a layer of cuboidal epithelial cells adjacent to the neurosensory retina and maintains the outer limit of the blood-retina barrier...
February 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28176468/correlation-of-pre-operative-plasma-protein-concentrations-in-cardiac-surgery-patients-with-bleeding-outcomes-using-a-targeted-quantitative-proteomics-approach
#3
Nathan Clendenen, Ashley Tollefson, Monika Dzieciatkowska, Alice Cambiaghi, Manuela Ferrario, Miranda Kroehl, Anirban Banerjee, Angelo D'Alessandro, Kirk C Hansen, Nathaen Weitzel
PURPOSE: Despite recent advancements in the use of thrombelastography (TEG) in the surgical setting, adequate technology to accurately predict bleeding phenotypes for patients undergoing cardiopulmonary bypass on the basis of non-mechanical parameters is lacking. While basic science and translational studies have provided key mechanistic insights about the protein components of coagulation cascades and regulatory mediators of hemostasis and fibrinolysis, targeted protein assays are still missing and the association of protein profiles to bleeding phenotypes and TEG readouts have yet to be discovered...
February 8, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28161100/the-future-of-omics-in-hypertension
#4
REVIEW
Gemma Currie, Christian Delles
Despite decades of research and clinical practice, the pathogenesis of hypertension remains incompletely understood, and blood pressure is often suboptimally controlled. "Omics" technologies allow the description of a large number of molecular features and have the potential to identify new factors that contribute to blood pressure regulation and how they interact. In this review, we focus on the potential of genomics, transcriptomics, proteomics, and metabolomics and explore their roles in unraveling the pathophysiology and diagnosis of hypertension, the prediction of organ damage and treatment response, and monitoring treatment effect...
December 7, 2016: Canadian Journal of Cardiology
https://www.readbyqxmd.com/read/28154374/precision-diagnostics-moving-towards-protein-biomarker-signatures-of-clinical-utility-in-cancer
#5
REVIEW
Carl A K Borrebaeck
Interest in precision diagnostics has been fuelled by the concept that early detection of cancer would benefit patients; that is, if detected early, more tumours should be resectable and treatment more efficacious. Serum contains massive amounts of potentially diagnostic information, and affinity proteomics has risen as an accurate approach to decipher this, to generate actionable information that should result in more precise and evidence-based options to manage cancer. To achieve this, we need to move from single to multiplex biomarkers, a so-called signature, that can provide significantly increased diagnostic accuracy...
February 3, 2017: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/28149930/ten-years-of-proteomics-in-bladder-cancer-progress-and-future-directions
#6
REVIEW
Maria Frantzi, Antonia Vlahou
Research efforts targeting the identification of bladder cancer biomarkers have been extensive during the past decade. Investigations have been performed at the genome, transcriptome, proteome, and metabolome levels and outputs have started appearing including the sketching of disease molecular subtypes. Proteins are directly linked to cell phenotype hence they accumulate special interest as both biomarkers and therapeutic targets. Multiple technical challenges exist, of the main, being the protein concentration vast dynamic range and presence of proteins in modified forms...
January 27, 2017: Bladder Cancer
https://www.readbyqxmd.com/read/28149928/evening-and-morning-alterations-in-obstructive-sleep-apnea-red-blood-cell-proteome
#7
Amélia Feliciano, Fátima Vaz, Cristina Valentim-Coelho, Vukosava M Torres, Rita Silva, Vesna Prosinecki, Bruno M Alexandre, Andreia Almeida, Catarina Almeida-Marques, Ana S Carvalho, Rune Matthiesen, Atul Malhotra, Paula Pinto, Cristina Bárbara, Deborah Penque
This article presents proteomics data referenced in [1] Using proteomics-based evaluation of red blood cells (RBCs), we have identified differentially abundant proteins associated with Obstructive Sleep Apnea Syndrome (OSA). RBCs were collected from peripheral blood of patients with moderate/severe OSA or snoring at pre- (evening) and post-night (morning) polysomnography, so that proteome variations between these time points could be assessed. RBC cytoplasmic fraction depleted of hemoglobin, using Hemovoid(™) system, were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), the 2D image software-based analyzed and relevant differentially abundant proteins identified by mass spectrometry (MS)...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28140733/integrated-metabonomic-proteomic-studies-on-blood-enrichment-effects-of-angelica-sinensis-on-a-blood-deficiency-mice-model
#8
Yongli Hua, Wangling Yao, Peng Ji, Yanming Wei
CONTEXT: Angelica sinensis (Oliv.) Diels (Umbelliferae) (AS) is a well-known Traditional Chinese Medicine (TCM) that enriches and regulates the blood. OBJECTIVE: An integrated metabonomic and proteomic method was developed and applied to study the blood enrichment effects and mechanisms of AS on blood deficiency (BD) mouse model. MATERIALS AND METHODS: Forty mice were randomly divided into the control, BD, High-dose of AS (ASH), Middle-dose of AS (ASM), and Low-dose of AS (ASL) groups...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28126963/plasma-derived-proteomic-biomarkers-in-hla-haploidentical-or-hla-matched-bone-marrow-transplantation-using-post-transplantation-cyclophosphamide
#9
Christopher G Kanakry, Giorgos Bakoyannis, Susan M Perkins, Shannon R McCurdy, Ante Vulic, Edus H Warren, Etienne Daguindau, Taylor Olmsted, Christen Mumaw, Andrea M H Towlerton, Kenneth R Cooke, Paul V O'Donnell, Heather J Symons, Sophie Paczesny, Leo Luznik
Recent studies have suggested that plasma-derived proteins may be potential biomarkers relevant for graft-versus-host disease and/or non-relapse mortality occurring after allogeneic blood or marrow transplantation. However, none of these putative biomarkers have been assessed in patients treated either with HLA-haploidentical blood or marrow transplantation or with post-transplantation cyclophosphamide, which has been repeatedly associated with low rates of severe acute graft-versus-host disease, chronic graft-versus-host disease, and non-relapse mortality...
January 25, 2017: Haematologica
https://www.readbyqxmd.com/read/28120209/differential-proteomics-analysis-of-endometriosis-in-blood-stasis-syndrome
#10
Yi Wen, Yan Wang, Ting-Ting Feng, Shao-Bin Wei
OBJECTIVE: To investigate the innate characters of 3 endometriosis (EMT) syndromes, blood stasis (BS), qi stagnation and blood stasis (QSBS) as well as Shen (Kidney) deficiency and blood stasis (KDBS) in terms of proteomics, lay a molecular biological basis for the differentiation of various blood stasis syndromes of EMT, establish a EMT microscopic syndrome differentiation and diagnosis system in terms of proteomics, discover the evolution principles and therapeutic targets of these EMT syndromes, and search their significant molecular markers and genetic intervention targets...
January 24, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28114394/proteomic-analysis-of-kveim-reagent-identifies-targets-of-cellular-immunity-in-sarcoidosis
#11
Christian Eberhardt, Muhunthan Thillai, Robert Parker, Nazneen Siddiqui, Lee Potiphar, Rob Goldin, John F Timms, Athol U Wells, Onn M Kon, Melissa Wickremasinghe, Donald Mitchell, Mark E Weeks, Ajit Lalvani
BACKGROUND: Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28112944/targeted-proteomics-predict-a-sustained-complete-response-after-transarterial-chemoembolization-and-clinical-outcomes-in-patients-with-hepatocellular-carcinoma-a-prospective-cohort-study
#12
Su Jong Yu, Hyunsoo Kim, Hophil Min, A-Reum Sohn, Young Youn Cho, Jeong-Ju Yoo, Dong Hyeon Lee, Eun Ju Cho, Jeong-Hoon Lee, Jungsoo Gim, Taesung Park, Yoon Jun Kim, Chung Yong Kim, Jung-Hwan Yoon, Youngsoo Kim
This study was aimed to identify blood-based biomarkers to predict a sustained complete response (CR) after transarterial chemoembolization (TACE) using targeted proteomics. Consecutive patients with HCC who had undergone TACE were prospectively enrolled [training (n=100) and validation set (n=80)]. Serum samples were obtained before and 6 months after TACE. Treatment responses were evaluated using the modified Response Evaluation Criteria in Solid Tumors (mRECIST). In the training set, the MRM-MS assay identified 5 MCPs (LRG1, APCS, BCHE, C7, and FCN3)...
January 23, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28112871/quantification-by-nano-liquid-chromatography-parallel-reaction-monitoring-mass-spectrometry-of-human-apolipoprotein-a-i-apolipoprotein-b-and-hemoglobin-a1c-in-dried-blood-spots
#13
Clark M Henderson, James G Bollinger, Jessica O Becker, Jennifer M Wallace, Thomas J Laha, Michael J MacCoss, Andrew N Hoofnagle
PURPOSE: Proteomic analysis of blood proteins in DBS is gaining attention as a possible replacement for measurements in plasma/serum collected by venipuncture. We aimed to develop and provisionally validate a nanoflow LC-PRM-MS method for clinical use. EXPERIMENTAL DESIGN: We used Skyline to develop a nanoflow LC-PRM-MS method to quantify glycated hemoglobin-β, apolipoprotein A-I, and apolipoprotein B in DBS. Precision, linearity, interferences, and stability and were determined and the method was used to analyze samples from 36 human volunteers...
January 23, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28109429/body-fluid-degradomics-and-characterization-of-basic-n-terminome
#14
F Sabino, O Hermes, U Auf dem Keller
Rapid improvements in instrumentation and data analysis make mass spectrometry-based proteomics the method of choice for global characterization of proteomes and discovery of protein-based biomarkers. On the contrary to tissue biopsies, body fluids-e.g., blood, wound fluid, urine, and saliva-are noninvasive and easy to collect and process. However, they are very complex and present high dynamic ranges of protein concentrations, rendering direct shotgun proteomics analysis as inefficient for identification of low-abundance proteins in these specimens...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28101531/osteopontin-is-a-blood-biomarker-for-microglial-activation-and-brain-injury-in-experimental-hypoxic-ischemic-encephalopathy
#15
Yikun Li, Eric B Dammer, Xiaohui Zhang-Brotzge, Scott Chen, Duc M Duong, Nicholas T Seyfried, Chia-Yi Kuan, Yu-Yo Sun
Clinical management of neonatal hypoxic-ischemic encephalopathy (HIE) suffers from the lack of reliable surrogate marker tests. Proteomic analysis may identify such biomarkers in blood, but there has been no proof-of-principle evidence to support this approach. Here we performed in-gel trypsin digestion of plasma proteins from four groups of 10-d-old mice [untouched and 24 h after low-dose lipopolysaccharide (LPS) exposure, hypoxia-ischemia (HI), or LPS/HI injury; n = 3 in each group) followed by liquid chromatography-tandem mass spectrometry and bioinformatics analysis to search for HI- and LPS/HI-associated brain injury biomarkers...
January 2017: ENeuro
https://www.readbyqxmd.com/read/28067231/multiomics-reveal-non-alcoholic-fatty-liver-disease-in-rats-following-chronic-exposure-to-an-ultra-low-dose-of-roundup-herbicide
#16
Robin Mesnage, George Renney, Gilles-Eric Séralini, Malcolm Ward, Michael N Antoniou
The impairment of liver function by low environmentally relevant doses of glyphosate-based herbicides (GBH) is still a debatable and unresolved matter. Previously we have shown that rats administered for 2 years with 0.1 ppb (50 ng/L glyphosate equivalent dilution; 4 ng/kg body weight/day daily intake) of a Roundup GBH formulation showed signs of enhanced liver injury as indicated by anatomorphological, blood/urine biochemical changes and transcriptome profiling. Here we present a multiomic study combining metabolome and proteome liver analyses to obtain further insight into the Roundup-induced pathology...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28062797/targeted-proteomics-for-multiplexed-verification-of-markers-of-colorectal-tumorigenesis
#17
Anuli Christiana Uzozie, Nathalie Selevsek, Asa Wahlander, Paolo Nanni, Jonas Grossmann, Achim Weber, Federico Buffoli, Giancarlo Marra
Targeted proteomic methods can accelerate the verification of multiple tumor marker candidates in large series of patient samples. We utilized the targeted approach known as selected / multiple reaction monitoring (SRM/MRM) to verify potential protein markers of colorectal adenoma identified by our group in previous transcriptomic and quantitative shotgun proteomic studies of a large cohort of precancerous colorectal lesions. We developed SRM assays to reproducibly detect and quantify 25 (62.5%) of the 40 selected proteins in an independent series of precancerous and cancerous tissue samples (19 adenoma/normal mucosa pairs; 17 adenocarcinoma/normal mucosa pairs)...
January 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27965111/sialylated-fetuin-a-as-a-candidate-predictive-biomarker-for-successful-grass-pollen-allergen-immunotherapy
#18
Noémie Caillot, Julien Bouley, Karine Jain, Sandrine Mariano, Sonia Luce, Stéphane Horiot, Sabi Airouche, Chloé Beuraud, Christian Beauvallet, Philippe Devillier, Sylvie Chollet-Martin, Christine Kellenberger, Laurent Mascarell, Henri Chabre, Thierry Batard, Emmanuel Nony, Vincent Lombardi, Véronique Baron-Bodo, Philippe Moingeon
BACKGROUND: Eligibility to immunotherapy is based on the determination of IgE reactivity to a specific allergen by skin prick or in vitro testing. Biomarkers predicting the likelihood of clinical improvement during immunotherapy would significantly improve patient selection. METHODS: Proteins were differentially assessed by 2D-DiGE and label-free mass spectrometry in pre-treatment sera obtained from clinical responders and non-responders within a cohort of 82 grass pollen allergic patients receiving sublingual immunotherapy or placebo...
December 10, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27943336/verification-of-protein-biomarkers-for-the-identification-of-biological-stains-by-quadrupole-time-of-flight-mass-spectrometry
#19
Kevin M Legg, Roger Powell, Nichole Reisdorph, Rick Reisdorph, Phillip B Danielson
Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analysis. Previous proteome studies compiled panels of "candidate biomarkers" specific to each of six body fluids (i.e. peripheral blood, menstrual fluid, vaginal fluid, seminal fluid, urine and saliva). Here, a multiplex Quadrupole Time-of-flight assay has been developed in order to verify the tissue/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a samples population of fifty humans...
December 12, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27943101/salivary-biomarkers-in-cancer-detection
#20
REVIEW
Xiaoqian Wang, Karolina Elżbieta Kaczor-Urbanowicz, David T W Wong
Cancer is the second most common cause of death in the USA. Its symptoms are often not specific and absent, until the tumors have already metastasized. Therefore, there is an urgent demand for developing rapid, highly accurate and noninvasive tools for cancer screening, early detection, diagnostics, staging and prognostics. Saliva as a multi-constituent oral fluid comprises secretions from the major and minor salivary glands, extensively supplied by blood. Molecules such as DNAs, RNAs, proteins, metabolites, and microbiota, present in blood, could be also found in saliva...
January 2017: Medical Oncology
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