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Blood biomarker proteomic

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https://www.readbyqxmd.com/read/29766829/acute-myeloid-leukemia-diagnosis-and-management-based-on-current-molecular-genetics-approach
#1
E Suguna, R Farhana, E Kanimozhi, P SaiKumar, G Kumaramanickavel, Chitralekha Sai Kumar
Background &Objective: Acute myeloid leukemia (AML) is characterized by accumulation of ?20% myeloid premature blast cells in the bone marrow and most often found in the peripheral blood. AML is generally classified based on two groups, namely, French-American-British (FAB) and World Health Organization (WHO) systems. For better clinical management, cytogenetic findings in AML are necessary and in patients with normal karyotypes, molecular analysis becomes critical. Mutations of certain genes like Nucleophosmin 1gene (NPM1), Fms-related Tyrosine Kinase 3 (FLT3), CCAAT/Enhancer Binding Protein Alpha (CEBPA), Runt-related transcription factor 1(RUNX1), and Mixed Lineage Leukemia (MLL) play a crucial role in the risk management and clinical stratification of AML patients...
May 15, 2018: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/29717677/facilitating-earlier-diagnosis-of-cardiovascular-disease-through-point-of-care-biosensors-a-review
#2
Mackenzie M Honikel, Chi-En Lin, David Probst, Jeffrey T La Belle
Cardiovascular disease (CVD) accounts for 30% of all global deaths and is predicted to dominate in the coming years, despite vast improvements in medical technology. Current clinical methods of assessing an individual's cardiovascular health include blood tests to monitor relevant biomarker levels as well as varying imaging modalities such as electrocardiograms, computed tomography, and angiograms to assess vasculature. As informative as these tools are, they each require lengthy scheduling, preparation, and highly trained personnel to interpret the results before any information is accessible to patients, often leading to delayed treatment, which can be fatal...
2018: Critical Reviews in Biomedical Engineering
https://www.readbyqxmd.com/read/29708539/protein-digestion-ultrafiltration-and-size-exclusion-chromatography-to-optimize-the-isolation-of-exosomes-from-human-blood-plasma-and-serum
#3
Gustavo Diaz, Chandler Bridges, Megan Lucas, Yong Cheng, Jeff S Schorey, Karen M Dobos, Nicole A Kruh-Garcia
Exosomes, a type of nanovesicle released from all cell types, can be isolated from any bodily fluid. The contents of exosomes, including proteins and RNAs, are unique to the cells from which they are derived and can be used as indicators of disease. Several common enrichment protocols, including ultracentrifugation, yield exosomes laden with soluble protein contaminants. Specifically, we have found that the most abundant proteins within blood often co-purify with exosomes and can confound downstream proteomic studies, thwarting the identification of low abundance biomarker candidates...
April 13, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29707944/high-field-asymmetric-waveform-ion-mobility-spectrometry-in-non-targeted-bottom-up-proteomics-of-dried-blood-spots
#4
Cecilie Rosting, Jinglei Yu, Helen J Cooper
Despite the great potential of Dried Blood Spots (DBS) as a source of endogenous proteins for biomarker discovery, the literature relating to non-targeted bottom-up proteomics of DBS is sparse, primarily due to the inherent complexity and very high dynamic range associated with these samples. Here, we present proof-of-concept results in which we have coupled high field asymmetric waveform ion mobility spectrometry (FAIMS) with LC-MS/MS for non-targeted bottom-up proteomics of DBS with the aim of addressing these challenges...
April 30, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29703324/label-free-quantitative-proteomics-reveals-fibrinopeptide-b-and-heparin-cofactor-ii-as-potential-serum-biomarkers-in-respiratory-syncytial-virus-infected-mice-treated-with-qingfei-oral-liquid-formula
#5
Li-Hua Zhou, Jian-Ya Xu, Chen Dai, Yi-Man Fan, Bin Yuan
Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections. Qingfei oral liquid (QFOL), a traditional Chinese medicine, is widely used in clinical treatment for RSV-induced pneumonia. The present study was designed to reveal the potential targets and mechanism of action for QFOL by exploring its influence on the host cellular network following RSV infection. We investigated the serum proteomic changes and potential biomarkers in an RSV-infected mouse pneumonia model treated with QFOL...
April 2018: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29695634/tumor-independent-host-secretomes-induced-by-angiogenesis-and-immune-checkpoint-inhibitors
#6
Michalis Mastri, Christina R Lee, Amanda Tracz, Robert S Kerbel, Melissa Dolan, Yuhao Shi, John M L Ebos
The levels of various circulating blood proteins can change in response to cancer therapy. Monitoring therapy-induced secretomes (TIS) may have use as biomarkers for establishing optimal biological effect (such as dosing) or identifying sources of toxicity and drug resistance. While TIS can derive from tumor cells directly, non-tumor 'host' treatment responses can also impact systemic secretory programs. For targeted inhibitors of the tumor microenvironment, including antiangiogenic and immune-checkpoint therapies, host TIS could explain unexpected collateral 'side-effects' of treatment...
April 25, 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29691073/microbiological-diagnosis-of-bacteraemia-and-fungaemia-blood-cultures-and-molecular-methods
#7
María Remedio Guna Serrano, Nieves Larrosa Escartín, Mercedes Marín Arriaza, Juan Carlos Rodríguez Díaz
OBJETIVE: In this review we try to update the new procedures applicable in the microbiological diagnosis of bacteriemia and fungemias. METHOD: Review of scientific literature. RESULTS AND CONCLUSIONS: After defining the process and indicating its fundamental principles, the main biomarkers used in clinical practice are reviewed. Subsequently, the particularities of the pre-analytical phase (collection and transport of samples) are highlighted and the steps to follow for the microbiological identification by classical methods are detailed, based on the culture of the blood samples...
April 21, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29689304/proteomics-of-liquid-biopsies-depicting-rcc-infiltration-into-the-renal-vein-by-ms-analysis-of-urine-and-plasma
#8
Clizia Chinello, Martina Stella, Isabella Piga, Andrew James Smith, Giorgio Bovo, Marta Varallo, Mariia Ivanova, Vanna Denti, Marco Grasso, Angelica Grasso, Marina Del Puppo, Apostolos Zaravinos, Fulvio Magni
Liquid biopsies, as blood and urine, could offer an invaluable, easily accessible source of biomarkers, and evidences for elucidating the pathological processes. Only few studies integrated the proteomes driven by more than one biofluid. Furthermore, it is not clear which biofluid better mirrors the alterations triggered by disease. Venous infiltrating RCC(Renal Cell Carcinoma) could represent an advantageous model for exploring this aspect. Herein, we investigate how blood and urine "proteomically" reflect the changes occurring during RCC infiltration into renal vein(RV) by label-free nLC-ESI-MS/MS...
April 21, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29677216/plasma-metabolomic-biomarkers-accurately-classify-acute-mild-traumatic-brain-injury-from-controls
#9
Massimo S Fiandaca, Mark Mapstone, Amin Mahmoodi, Thomas Gross, Fabio Macciardi, Amrita K Cheema, Kian Merchant-Borna, Jeffrey Bazarian, Howard J Federoff
Past and recent attempts at devising objective biomarkers for traumatic brain injury (TBI) in both blood and cerebrospinal fluid have focused on abundance measures of time-dependent proteins. Similar independent determinants would be most welcome in diagnosing the most common form of TBI, mild TBI (mTBI), which remains difficult to define and confirm based solely on clinical criteria. There are currently no consensus diagnostic measures that objectively define individuals as having sustained an acute mTBI. Plasma metabolomic analyses have recently evolved to offer an alternative to proteomic analyses, offering an orthogonal diagnostic measure to what is currently available...
2018: PloS One
https://www.readbyqxmd.com/read/29672914/revisiting-the-nih-taskforce-on-the-research-needs-of-eosinophil-associated-diseases-re-tread
#10
REVIEW
Paneez Khoury, Praveen Akuthota, Steven J Ackerman, Joseph R Arron, Bruce S Bochner, Margaret H Collins, Jean-Emmanuel Kahn, Patricia C Fulkerson, Gerald J Gleich, Rashmi Gopal-Srivastava, Elizabeth A Jacobsen, Kristen M Leiferman, Levi-Schaffer Francesca, Sameer K Mathur, Michael Minnicozzi, Calman Prussin, Marc E Rothenberg, Florence Roufosse, Kathleen Sable, Dagmar Simon, Hans-Uwe Simon, Lisa A Spencer, Jonathan Steinfeld, Andrew J Wardlaw, Michael E Wechsler, Peter F Weller, Amy D Klion
BACKGROUND: Eosinophil-associated diseases (EADs) are rare, heterogeneous disorders characterized by the presence of eosinophils in tissues and/or peripheral blood resulting in immunopathology. The heterogeneity of tissue involvement, lack of sufficient animal models, technical challenges in working with eosinophils, and lack of standardized histopathologic approaches have hampered progress in basic research. Additionally, clinical trials and drug development for rare EADs are limited by the lack of primary and surrogate endpoints, biomarkers, and validated patient-reported outcomes...
April 19, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29670052/aneuploid-ctc-and-cec
#11
REVIEW
Peter Ping Lin
Conventional circulating tumor cell (CTC) detection technologies are restricted to large tumor cells (> white blood cells (WBCs)), or those unique carcinoma cells with double positive expression of surface epithelial cell adhesion molecule (EpCAM) for isolation, and intracellular structural protein cytokeratins (CKs) for identification. With respect to detecting the full spectrum of highly heterogeneous circulating rare cells (CRCs), including CTCs and circulating endothelial cells (CECs), it is imperative to develop a strategy systematically coordinating all tri-elements of nucleic acids, biomarker proteins, and cellular morphology, to effectively enrich and comprehensively identify CRCs...
April 18, 2018: Diagnostics
https://www.readbyqxmd.com/read/29669750/current-challenges-and-opportunities-in-treating-glioblastoma
#12
Andrea Shergalis, Armand Bankhead, Urarika Luesakul, Nongnuj Muangsin, Nouri Neamati
Glioblastoma multiforme (GBM), the most common and aggressive primary brain tumor, has a high mortality rate despite extensive efforts to develop new treatments. GBM exhibits both intra- and intertumor heterogeneity, lending to resistance and eventual tumor recurrence. Large-scale genomic and proteomic analysis of GBM tumors has uncovered potential drug targets. Effective and "druggable" targets must be validated to embark on a robust medicinal chemistry campaign culminating in the discovery of clinical candidates...
July 2018: Pharmacological Reviews
https://www.readbyqxmd.com/read/29663493/mouse-serum-exosomal-proteomic-signature-in-response-to-asbestos-exposure
#13
Phillip Munson, Ying-Wai Lam, Maximilian MacPherson, Stacie Beuschel, Arti Shukla
Asbestos-induced diseases like fibrosis and mesothelioma are very aggressive, without any treatment options. These diseases are diagnosed only at the terminal stages due to lack of early stage biomarkers. The recent discovery of exosomes as circulating biomarkers led us to look for exosomal biomarkers of asbestos exposure in mouse blood. In our model, mice were exposed to asbestos as a single bolus dose by oropharyngeal aspiration. Fifty-six days later blood was collected, exosomes were isolated from plasma and characterized and subjected to proteomic analysis using Tandem Mass Tag labeling...
April 16, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29660691/proteomic-analysis-of-liquid-biopsy-from-tumor-draining-vein-indicates-that-high-expression-of-exosomal-ecm1-is-associated-with-relapse-in-stage-i-iii-colon-cancer
#14
Sandra Santasusagna, Isabel Moreno, Alfons Navarro, Joan J Castellano, Francisco Martinez, Raquel Hernández, Carmen Muñoz, Mariano Monzo
BACKGROUND: The analysis of exosomes in blood obtained from the tumor-draining mesenteric vein (MV) can identify tumor biomarkers before they reach target organs and form the premetastatic niche where circulating tumor cells can anchor. Our group has recently shown that microRNAs in plasma from the MV-but not the peripheral vein (PV)-have been related to liver metastases in colon cancer (CC) patients. Here we examine the exosomal protein cargo in plasma from the MV and paired PV in 31 CC patients...
April 13, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29658936/two-dimensional-gel-electrophoresis-coupled-with-mass-spectrometry-methods-for-an-analysis-of-human-pituitary-adenoma-tissue-proteome
#15
Xianquan Zhan, Yuda Huang, Ying Long
Human pituitary adenoma (PA) is a common tumor that occurs in the human pituitary gland in the hypothalamus-pituitary-targeted organ axis systems, and may be classified as either clinically functional or nonfunctional PA (FPA and NFPA). NFPA is difficult for early stage diagnosis and therapy due to barely elevating hormones in the blood compared to FPA. Our long-term goal is to use proteomics methods to discover reliable biomarkers for clarification of PA molecular mechanisms and recognition of effective diagnostic, prognostic markers and therapeutic targets...
April 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29625564/urinary-proteomics-for-prediction-of-mortality-in-patients-with-type-2-diabetes-and-microalbuminuria
#16
Gemma E Currie, Bernt Johan von Scholten, Sheon Mary, Jose-Luis Flores Guerrero, Morten Lindhardt, Henrik Reinhard, Peter K Jacobsen, William Mullen, Hans-Henrik Parving, Harald Mischak, Peter Rossing, Christian Delles
BACKGROUND: The urinary proteomic classifier CKD273 has shown promise for prediction of progressive diabetic nephropathy (DN). Whether it is also a determinant of mortality and cardiovascular disease in patients with microalbuminuria (MA) is unknown. METHODS: Urine samples were obtained from 155 patients with type 2 diabetes and confirmed microalbuminuria. Proteomic analysis was undertaken using capillary electrophoresis coupled to mass spectrometry to determine the CKD273 classifier score...
April 6, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29625113/exosomal-proteome-analysis-of-human-plasma-to-monitor-sepsis-progression
#17
Yan Xu, Xin Ku, Chunrong Wu, Chunlin Cai, Jianguo Tang, Wei Yan
Exosomes are cell-derived vesicles containing RNA, lipid, and protein, which act in body immune response, intercellular signaling and some other important biological processes. Exosomes have been extensively studied in the past several years on their disease related mechanisms and potential roles to monitor disease progression as biomarkers. Compared with analyzing exosome RNA, comprehensive proteome profiling of exosomes in clinical samples (e.g. blood) are highly demanded but limited mainly due to lack of a reproducible method for efficient exosome extraction...
May 23, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29622549/biomarkers-for-post-transplantation-outcomes
#18
Sophie Paczesny
During the last decade, the development of biomarkers for the complications seen after allogeneic hematopoietic stem cell transplantation (allo-HSCT) has expanded tremendously with the most progress been made for acute graft-versus-host disease (aGVHD), a common and often fatal complication. Although many factors are known to determine transplant outcome (including the age of the recipient, comorbidity, conditioning intensity, donor source, donor-recipient human leukocyte antigen (HLA) compatibility, conditioning regimen, post-transplant GVHD prophylaxis), they are incomplete guides for predicting outcomes...
April 5, 2018: Blood
https://www.readbyqxmd.com/read/29621665/effect-of-sex-and-ryr1-gene-mutation-on-the-muscle-proteomic-profile-and-main-physiological-biomarkers-in-pigs-at-slaughter
#19
Mamen Oliván, Joel González, Anna Bassols, Fernando Díaz, Ricard Carreras, Eva Mainau, Laura Arroyo, Raquel Peña, Yaiza Potes, Ana Coto-Montes, Kristin Hollung, Antonio Velarde
Gender and RYR1 gene mutation might have an effect on the muscle metabolic characteristics and on the animal's stress at slaughter, which could influence the process of muscle-to-meat conversion. Forty-eight pigs were distributed in a design including two factors: sex (male/female) and RYR1 genotype (NN/Nn). At slaughter, physiological blood biomarkers and muscle proteome were analyzed and carcass and meat quality traits were registered. Females had higher serum levels of glucose, urea, C-reactive protein "CRP", Pig-MAP and glutation-peroxidase "GPx" and lower levels of lactate, showed faster muscle pH decline and higher meat exudation...
March 27, 2018: Meat Science
https://www.readbyqxmd.com/read/29615349/assessment-of-extracellular-matrix-related-biomarkers-in-patients-with-lower-extremity-artery-disease
#20
Anna Hernández-Aguilera, Signe Holm Nielsen, Cristina Bonache, Salvador Fernández-Arroyo, Vicente Martín-Paredero, Montserrat Fibla, Morten A Karsdal, Federica Genovese, Javier A Menendez, Jordi Camps, Jorge Joven
BACKGROUND: The prevalence of lower extremity artery disease (LEAD) is high (20%-25%) in the population older than 65 years, but patients are seldom identified until the disease is advanced. Circulating markers of disease activity might provide patients with a key opportunity for timely treatment. We tested the hypothesis that measuring blood-specific fragments generated during degradation of the extracellular matrix (ECM) could provide further insight into the pathophysiologic mechanism of arterial remodeling...
March 31, 2018: Journal of Vascular Surgery
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