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Liver transplantation in rat

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https://www.readbyqxmd.com/read/28916482/disposition-of-treosulfan-and-its-active-monoepoxide-in-a-bone-marrow-liver-lungs-brain-and-muscle-studies-in-a-rat-model-with-clinical-relevance
#1
Michał Romański, Anna Kasprzyk, Mateusz Walczak, Agnieszka Ziółkowska, Franciszek Główka
For the recent years, the application of treosulfan (TREO)-based conditioning prior to hematopoietic stem cell transplantation (HSCT) has been increasing as an alternative to busulfan-based therapy, especially for patients presenting high risk of developing hepato-, pulmo-, and neurotoxicity. So far, the penetration of TREO and its epoxy-derivatives into central nervous system and aqueous humor of the eye has been investigated. However, lacking knowledge on the compounds distribution into the other key tissues precludes comprehensive understanding and assessment of TREO clinical efficacy and toxicity...
September 12, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28901794/silybin-against-liver-ischemia-reperfusion-injury-something-old-something-new%C3%A2
#2
Mihai Oltean
Ischemia reperfusion injury (IRI) is a life threatening condition that may develop after elective liver surgery or liver transplantation. Numerous surgical and pharmacological approaches have shown varying degrees of protection against liver IRI. A group of protective compounds are the flavonoids but their intestinal absorbtion and bioavailability are low and impredictible. In this issue Tsaroucha et al. reports significantly decreased hepatocellular injury, Fas/FasL expression and inhibited HMGB1 release in rats receiving a hydrosoluble, lyophilized complex of SLB and hydroxypropyl-β-cyclodextrin (SLB-HP-β-CD) intravenously...
September 13, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28901391/protective-effects-of-heme-oxygenase-1-transduced-bone-marrow-derived-mesenchymal-stem-cells-on-reduced%C3%A2-size-liver-transplantation-role-of-autophagy-regulated-by-the-erk-mtor-signaling-pathway
#3
Raorao Wang, Zhongyang Shen, Liu Yang, Mingli Yin, Weiping Zheng, Bin Wu, Tao Liu, Hongli Song
Autophagy is a critical lysosomal pathway that degrades cytoplasmic components to maintain cell homeostasis and provide substrates for energy metabolism. A study revealed that heme oxygenase-1 (HO-1)-transduced bone marrow-derived mesenchymal stem cells (BM-MSCs) could protect 50% reduced-size liver transplantation (RSLT) in a rat model. However, the mechanisms remain mostly unknown. The aim of the present study was to explore the effects and related mechanism of autophagy on the protection conferred by HO-1-transduced BM-MSCs (HO-1/BM-MSCs) on 50% RSLT in a rat model...
September 6, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28901189/cryopreservation-of-hepatocyte-microbeads-for-clinical-transplantation
#4
Suttiruk Jitraruch, Anil Dhawan, Robin D Hughes, Celine Filippi, Sharon C Lehec, Leanne Glover, Ragai R Mitry
Intraperitoneal transplantation of hepatocyte microbeads is an attractive option for the management of acute liver failure. Encapsulation of hepatocytes in alginate microbeads supports their function and prevents immune attack of the cells. Establishment of banked cryopreserved hepatocyte microbeads is important for emergency use. The aim of this study was to develop an optimized protocol for cryopreservation of hepatocyte microbeads for clinical transplantation using modified freezing solutions. Four freezing solutions with potential for clinical application were investigated...
August 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28889961/analysis-of-the-reversibility-of-biliary-cirrhosis-in-young-rats-submitted-to-biliary-obstruction
#5
Maria Julia de Aro Braz, Leonardo Ervolino Corbi, Ana Cristina Aoun Tannuri, Maria Cecília Mendonça Coelho, Josiane Oliveira Gonçalves, Suellen Serafini, Uenis Tannuri
BACKGROUND/PURPOSE: Biliary atresia and other liver biliary obstructions are relevant conditions in pediatric surgery due to their progression to biliary cirrhosis and indication for liver transplantation. It is known that the period during which biliary obstruction persists determines the development of cirrhosis and its reversibility after a biliary drainage procedure. However, no time or histological markers of biliary cirrhosis reversibility have been established. MATERIALS AND METHODS: One hundred and twenty-nine young Wistar rats underwent surgery for ligation of the common bile duct and were maintained until 8weeks...
August 24, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28887231/therapeutic-activity-of-superoxide-dismutase-containing-enzymosomes-on-rat-liver-ischaemia-reperfusion-injury-followed-by-magnetic-resonance-microscopy
#6
Paulo Marcelino, H Susana Marinho, Maria Celeste Campos, Ana Rita Neves, Carla Real, Filipa S Fontes, Alexandra Carvalho, Gabriel Feio, M Bárbara F Martins, M Luísa Corvo
Liver ischaemia-reperfusion injury (IRI) may occur during hepatic surgery and is unavoidable in liver transplantation. Superoxide dismutase enzymosomes (SOD-enzymosomes), liposomes where SOD is at the liposomal surface expressing enzymatic activity in intact form without the need of liposomal disruption, were developed with the aim of having a better insight into its antioxidant therapeutic outcome in IRI. We also aimed at validating magnetic resonance microscopy (MRM) at 7T as a tool to follow IRI. SOD-enzymosomes were characterized and tested in a rat ischaemia-reperfusion model and the therapeutic outcome was compared with conventional long circulating SOD liposomes and free SOD using biochemical liver injury biomarkers, histology and MRM...
September 6, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28885495/isoglycyrrhizinate-magnesium-enhances-hepatoprotective-effect-of-fk506-on-ischemia-reperfusion-injury-through-hmgb1-inhibition-in-a-rat-model-of-liver-transplantation
#7
Zhang Weichen, Li Feibo, Ye Yufu, Liu Yuanxing, Yu Songfeng, Cen Chao, Chen Xuliang, Zhou Lin, Tang Xiaofeng, Yu Jun, Zheng Shusen
BACKGROUND: Ischemia-reperfusion injury (IRI) after liver transplantation impairs graft function and affects prognosis of recipients. Isoglycyrrhizinate magnesium (Iso) is a hepatoprotective drug usually used after liver injury. In this study, we intended to explore whether Iso alone have protective effect after IRI in a rat model of liver transplantataion. We also aimed to study whether Iso could enhance the hepatoprotective effect of FK506 (tacrolimus) and underlying mechanism. METHODS: Rats after liver transplantation were treated with different concentration of FK506 with or without, Iso or lower-dose FK506 plus Iso...
September 6, 2017: Transplantation
https://www.readbyqxmd.com/read/28871615/controlled-oxygenated-rewarming-up-to-normothermia-for-pre-transplant-reconditioning-of-liver-grafts
#8
Charlotte von Horn, Hideo Baba, Patrik Hannaert, Thierry Hauet, Henri Leuvenink, Andreas Paul, Thomas Minor
Controlled oxygenated rewarming (COR) up to 20°C during ex vivo machine perfusion limits reperfusion induced tissue injury upon graft implantation. Rewarming up to normothermia might add further benefits and provide better prediction of post transplantation organ function. The effect of 90 min of oxygenated machine perfusion with Aqix RS-I after cold storage combined with gentle rewarming up to 20°C (COR20) or 35°C (COR35) was studied in rat livers and compared with cold storage alone (CS, n=6, resp). Post-preservation recovery was evaluated upon warm reperfusion using an established in vitro system...
September 4, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28870982/understanding-the-effect-of-corticosteroid-pretreatment-in-brain-dead-organ-donors-new-mechanistic-insights-for-improvement-of-organ-quality-in-liver-transplantation
#9
Carola Dahrenmöller, Raymond Reding
Transplant surgeons are currently faced with the challenge to accept marginal liver transplants due to steatosis or old age. Improving organ quality by implementing a selective organ protective donor management could be the first step towards a graft of enhanced quality. However, the molecular mechanisms of such treatments are still poorly understood. Glucocorticoid medication in donor medicine has been carried out and discussed for a long time. In a recent study published in Clinical Science, Jiménez-Castro et al...
September 15, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28870676/hypothermic-oxygenated-perfusion-hope-for-fatty-liver-grafts-in-rats-and-humans
#10
P Kron, A Schlegel, L Mancina, P-A Clavien, P Dutkowski
BACKGROUND: Pretreatment of marginal organs by perfusion is a promising opportunity to consider more organs for transplantation. Protection of human DCD (donation after cardiac death) livers by a novel machine perfusion technique, hypothermic oxygenated perfusion (HOPE), was recently established. Here we tested in a rodent transplant model, whether HOPE is also useful for fatty liver grafts. METHODS: Rats were fed over 3 weeks with a special Methionine-Choline-Deficient-Diet (MCDD) to induce severe hepatic macrosteatosis (≥60%)...
September 1, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28870599/hepatocyte-nuclear-factor-4a-improves-hepatic-differentiation-of-immortalized-adult-human-hepatocytes-and-improves-liver-function-and-survival
#11
Hua-Lian Hang, Xin-Yu Liu, Hai-Tian Wang, Ning Xu, Jian-Min Bian, Jian-Jun Zhang, Lei Xia, Qiang Xia
Immortalized human hepatocytes (IHH) could provide an unlimited supply of hepatocytes, but insufficient differentiation and phenotypic instability restrict their clinical application. This study aimed to determine the role of hepatocyte nuclear factor 4A (HNF4A) in hepatic differentiation of IHH, and whether encapsulation of IHH overexpressing HNF4A could improve liver function and survival in rats with acute liver failure (ALF). Primary human hepatocytes were transduced with lentivirus-mediated catalytic subunit of human telomerase reverse transcriptase (hTERT) to establish IHH...
September 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28864291/functional-recovery-upon-human-dental-pulp-stem-cell-transplantation-in-a-diabetic-neuropathy-rat-model
#12
Indrani Datta, Naini Bhadri, Pradnya Shahani, Debanjana Majumdar, Sowmithra Sowmithra, Rema Razdan, Ramesh Bhonde
Diabetic neuropathy (DN) is among the most debilitating complications of diabetes. Here, we investigated the effects of human dental pulp stem cell (DPSC) transplantation in Streptozotocin (STZ)-induced neuropathic rats. Six weeks after STZ injection, DPSCs were transplanted through two routes, intravenous (IV) or intramuscular (IM), in single or two repeat doses. Two weeks after transplantation, a significant improvement in hyperalgesia, grip-strength, motor coordination and nerve conduction velocity was observed in comparison with controls...
August 29, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28855128/inflammasome-activation-involved-in-early-inflammation-reaction-after-liver-transplantation
#13
Bao-Jian Hong, Hui Liu, Zhou-Han Wang, Yue-Xia Zhu, Li-Yun Su, Min-Xia Zhang, Ke Xu, Jian-Zhong Chen
Liver transplantation has emerged as a vital therapy for end-stage liver diseases. Acute -phase inflammation play an important role in liver graft injury.Recent studies have revealed that inflammasome are responsible for initiating inflammation in early stage of acute organ rejection in liver transplantation, however the underlying mechanism remains unclear. Here we explored to block inflammasome activation to see whether it can alleviate early inflammation reaction during rejection of allgrafts in a rat model and gain further insights into the mechanism of inhibiting inflammation in allografts...
September 5, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28833919/il-10-secreted-by-mesenchymal-stem-cells-attenuates-acute-liver-failure-through-inhibiting-pyroptosis
#14
Wang Jinglin, Ren Haozhen, Yuan Xianwen, Ma Hucheng, Shi Xiaolei, Ding Yitao
AIMS: Recently, the benefit of mesenchymal stem cells (MSCs) as a cell-based therapy for acute liver failure (ALF) has gained much attention, although the mechanism of action of MSCs in the treatment of ALF remains elusive. Pyroptosis is a novel form of programmed cell death with an intense inflammatory response. The aim of the present study was to explore the soluble cytokines secreted by MSCs and their therapeutic effects via inhibiting pyroptosis in ALF. METHODS: MSCs obtained from C57BL/6 mice were isolated and cultured according to an established protocol...
August 22, 2017: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/28808225/congestive-preconditioning-by-portal-venous-clamping-a-novel-preventive-concept-for-small-intestinal-congestive-re-outflow-injury
#15
Hironori Hayashi, Hiroyuki Takamura, Ryosuke Gabata, Yoshinao Ohbatake, Shinichi Nakanuma, Tomoharu Miyashita, Hidehiro Tajima, Itasu Ninomiya, Sachio Fushida, Tetsuo Ohta
BACKGROUND Congestion of the small intestine, which induces mucosal damage via apoptosis, is a common pathophysiological change in hepato-biliary-pancreatic surgery, including liver transplantation. Small intestinal mucosal damage can trigger postoperative complications via various pathways. Therefore, we investigated the efficacy of intermittent portal venous clamping, which we named congestive preconditioning (CPC), for decreasing congestive re-outflow injury (CRoI) of the small intestine. MATERIAL AND METHODS Small intestinal CRoI was induced in rats by clamping the portal vein (PV) for 30 min, followed by re-outflow...
August 15, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28807057/inhibitory-effects-of-hnf4%C3%AE-on-migration-maltransformation-of-hepatic-progenitors-hnf4%C3%AE-overexpressing-hepatic-progenitors-for-liver-repopulation
#16
Ping Wang, Min Cong, Tianhui Liu, Hufeng Xu, Lin Wang, Guangyong Sun, Aiting Yang, Dong Zhang, Jian Huang, Yameng Sun, Wenshan Zhao, Hong Ma, Jidong Jia, Hong You
BACKGROUND: Although they are expandable in vitro, hepatic progenitors are immature cells and share many immunomarkers with hepatocellular carcinoma, raising potential concerns regarding maltransformation after transplantation. This study investigated the effects of hepatic nuclear factor (HNF) 4α on the proliferation, migration, and maltransformation of hepatic progenitors and determined the feasibility of using these manipulated cells for transplantation. METHODS: The effects of HNF4α on rat hepatic progenitors (i...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28749819/engraftment-and-repopulation-potential-of-late-gestation-fetal-rat-hepatocytes
#17
Joan M Boylan, Heather Francois-Vaughan, Philip A Gruppuso, Jennifer A Sanders
BACKGROUND: The limited availability of donor organs has led to a search for alternatives to liver transplantation to restore liver function and bridge patients to transplantation. We have shown that the proliferation of late gestation (embryonic day 19) fetal rat hepatocytes is mitogen-independent and that mechanisms regulating mRNA translation, cell cycle progression, and gene expression differ from those of adult rat hepatocytes. In the present study, we investigated whether E19 fetal hepatocytes can engraft and repopulate an injured adult liver...
October 2017: Transplantation
https://www.readbyqxmd.com/read/28748913/d-chiro-inositol-effectively-attenuates-cholestasis-in-bile-duct-ligated-rats-by-improving-bile-acid-secretion-and-attenuating-oxidative-stress
#18
Shuang-Shuang Zhao, Na-Ren Li, Wu-Li Zhao, Hong Liu, Mao-Xu Ge, Yi-Xuan Zhang, Long-Yin Zhao, Xue-Fu You, Hong-Wei He, Rong-Guang Shao
Cholestatic liver diseases are important causes of liver cirrhosis and liver transplantation, but few drugs are available for treatment. D-chiro-inositol (DCI), an isomer of inositol found in many Leguminosae plants and in animal viscera, is used clinically for the treatment of polycystic ovary syndrome (PCOS) and diabetes mellitus. In this study, we investigated whether DCI exerted an anti-cholestatic effect and its underlying mechanisms. A cholestatic rat model was established via bile duct ligation (BDL)...
July 27, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28735385/preparation-of-decellularized-liver-scaffolds-and-recellularized-liver-grafts
#19
Yibin Chen, Sharon Geerts, Maria Jaramillo, Basak E Uygun
Severe liver disease is the 12th leading cause of death in the USA, with organ transplantation often being the only viable option for treatment. However, due to the shortage of viable donor livers, it is estimated that over 1200 patients died in 2015 while waiting for liver transplantation. This highlights the need for alternative sources of viable organs. In this study, we describe a method that provides the groundwork for the development of functional liver grafts. The approach described here is for removal of cells from intact livers and subsequently repopulating them with functional liver cells...
July 23, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28723980/preliminary-results-of-topical-hepatic-hypothermia-in-a-model-of-liver-ischemia-reperfusion-injury-in-rats
#20
Emilio Elias Abdo, Estela Regina Ramos Figueira, Joel Avancini Rocha-Filho, Eleazar Chaib, Luiz Augusto Carneiro D'Albuquerque, Telesforo Bacchella
BACKGROUND: Ischemia/reperfusion causes organ damage but it is mandatory in hepatic transplantation, trauma and other complex liver surgeries, when Pringle maneuver is applied to minimize bleeding during these procedures. It is well known that liver ischemia/reperfusion leads to microcirculatory disturbance and cellular injury. In this setting hypothermia is known to reduce oxygen demand, lowering intracellular metabolism. OBJECTIVE: To evaluate the effects of hypothermia in liver ischemia/reperfusion injury, using a new model of topic isolated liver hypothermia...
July 6, 2017: Arquivos de Gastroenterologia
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