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Liver transplantation in rat

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https://www.readbyqxmd.com/read/29771932/a-multi-center-preclinical-study-of-gadoxetate-dce-mri-in-rats-as-a-biomarker-of-drug-induced-inhibition-of-liver-transporter-function
#1
Anastassia Karageorgis, Stephen C Lenhard, Brittany Yerby, Mikael F Forsgren, Serguei Liachenko, Edvin Johansson, Mark A Pilling, Richard A Peterson, Xi Yang, Dominic P Williams, Sharon E Ungersma, Ryan E Morgan, Kim L R Brouwer, Beat M Jucker, Paul D Hockings
Drug-induced liver injury (DILI) is a leading cause of acute liver failure and transplantation. DILI can be the result of impaired hepatobiliary transporters, with altered bile formation, flow, and subsequent cholestasis. We used gadoxetate dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), combined with pharmacokinetic modelling, to measure hepatobiliary transporter function in vivo in rats. The sensitivity and robustness of the method was tested by evaluating the effect of a clinical dose of the antibiotic rifampicin in four different preclinical imaging centers...
2018: PloS One
https://www.readbyqxmd.com/read/29768493/remote-ischemic-preconditioning-stat3-dependently-ameliorates-pulmonary-ischemia-reperfusion-injury
#2
Nanfu Luo, Jin Liu, Yan Chen, Huan Li, Zhaoyang Hu, Geoffrey W Abbott
The lungs are highly susceptible to injury, including ischemia/reperfusion (I/R) injury. Pulmonary I/R injury can occur when correcting conditions such as primary pulmonary hypertension, and is also relatively common after lung transplantation or other cardiothoracic surgery. Methods to reduce pulmonary I/R injury are urgently needed to improve outcomes following procedures such as lung transplantation. Remote liver ischemic preconditioning (RLIPC) is an effective cardioprotective measure, reducing damage caused by subsequent cardiac I/R injury, but little is known about its potential role in pulmonary protection...
2018: PloS One
https://www.readbyqxmd.com/read/29768256/overexpression-of-pck1-gene-antagonizes-hepatocellular-carcinoma-through-the-activation-of-gluconeogenesis-and-suppression-of-glycolysis-pathways
#3
Yufu Tang, Yibing Zhang, Chunhui Wang, Zhongyi Sun, Longfei Li, Shuqun Cheng, Wenping Zhou
BACKGROUND/AIMS: Gluconeogenesis, a reverse process of glycolysis, is suppressed in neoplastic livers. Cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C/PCK1, encoded by PCK1) is a step limiting enzyme of gluconeogenesis. The induced expression of the factor is reported to initiate gluconeogenesis process and antagonize hepatocellular carcinoma (HCC). In the current study, the effect of the modulation of PCK1 expression on HCC was assessed. METHODS: The levels of PCK1 in clinical HCC tissues and different HCC cell lines were investigated with real time quantitative PCR, immunochemistry, and western blotting...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29761416/bioluminescence-imaging-of-transplanted-mesenchymal-stem-cells-by-overexpression-of-hepatocyte-nuclear-factor4%C3%AE-tracking-biodistribution-and-survival
#4
Peiyi Xie, Xiaojun Hu, Dan Li, Sidong Xie, Zhiyang Zhou, Xiaochun Meng, Hong Shan
PURPOSE: The purposes of this study were to construct immortalized human bone marrow mesenchymal stem cells (UE7T-13) with overexpression of the hepatocyte nuclear factor4α (hHNF4α) and luciferase2-mKate2 dual-fusion reporter gene, further investigate their impact on treating acute liver injury (ALI) in rats, and track their biodistribution and survival by bioluminescence imaging (BLI). PROCEDURES: The hHNF4α and luciferase2-mKate2 genes were transduced by a lentiviral vector into UE7T-13 cells (named E7-hHNF4α-R cells), and expression was verified by immunofluorescence, RT-PCR, and flow cytometry...
May 14, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29755641/ischemic-preconditioning-attenuates-acute-lung-injury-after-partial-liver-transplantation
#5
Qinlong Liu, Hasibur Rehman, Yasodha Krishnasamy, John J Lemasters, Zhi Zhong
Pulmonary complications frequently occur after liver transplantation and are often life-threatening. Thus, we investigated whether hepatic ischemic preconditioning (IP) attenuates acute lung injury (ALI) after small-for-size liver transplantation. Rat livers were explanted after 9-min ischemia plus 5-min reperfusion, reduced to 50% of original size ex vivo , and implanted into recipients with approximately twice the donor body weight, resulting in quarter-size liver grafts (QSG). After QSG transplantation, hepatic Toll-like receptor 4 (TLR4) and tumor necrosis factor-α (TNFα ) expression increased markedly and high mobility group box-1 (HMGB1), an endogenous damage-associated molecular pattern molecule (DAMP), was released from QSG into the blood...
2018: International Journal of Physiology, Pathophysiology and Pharmacology
https://www.readbyqxmd.com/read/29749104/soluble-fibrinogen-like-protein-2-ameliorates-acute-rejection-of-liver-transplantation-in-rat-via-inducing-kupffer-cells-m2-polarization
#6
Guangrui Pan, Zhengfei Zhao, Chengyong Tang, Liuyue Ding, Zhongtang Li, Daofeng Zheng, Liang Zong, Zhongjun Wu
Soluble fibrinogen-like protein 2 (sFGL2) could ameliorate acute rejection (AR) in rat cardiac transplantation. However, the role of sFGL2 in AR of liver transplantation has not been addressed. In this study, we found that FGL2 was upregulated in rat orthotropic liver transplantation (OLT) models of tolerance and positive correlation with the frequency of M2 Kupffer cells (KCs). Gain-of-function experiments in vitro showed that sFGL2 promoted the secretion of anti-inflammatory cytokines (IL-10, TGF-β) and the expression of CD206, and inhibited the activities of STAT1 and NF-κB signaling pathway...
May 10, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29731771/undifferentiated-adipose-tissue-stem-cell-transplantation-promotes-hepatic-regeneration-ameliorates-histopathologic-damage-of-the-liver-and-upregulates-the-expression-of-liver-regeneration-and-liver-specific-genes-in-a-rat-model-of-partial-hepatectomy
#7
Konstantinos G Apostolou, Ioannis G Papanikolaou, Charalampos Katselis, Themistoklis Feretis, Dimitrios Kletsas, Manousos M Konstadoulakis, Maria Lymperi, Angelica A Saetta, Spiros Tsikalakis, George Agrogiannis, Efstratios Patsouris, George C Zografos, Apostolos E Papalois
Objective: Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and liver-specific genes, following 60% partial hepatectomy (PHx). Methods: Sixty female rats were subjected to PHx and were transplanted with 106 or 2 × 106 ADSCs, either into the portal vein (PV) or into the hepatic parenchyma...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29723851/transplantation-of-telocytes-attenuates-unilateral-ureter-obstruction-induced-renal-fibrosis-in-rats
#8
Long Zheng, Long Li, Guisheng Qi, Mushuang Hu, Chao Hu, Shuo Wang, Jiawei Li, Mingnan Zhang, Weitao Zhang, Yigang Zeng, Yi Zhang, Liping Li, Xuanchuan Wang, Miao Lin, Tongyu Zhu, Ruiming Rong
BACKGROUND/AIMS: Previous studies imply that telocytes may have a protective effect on fibrosis in various organs, including the liver, colon, and heart. The effect of telocytes on renal fibrosis remains unknown. Herein, this study was designed to investigate the effect of telocytes on renal fibrosis and the potential mechanisms involved. METHODS: In a unilateral ureteral obstruction (UUO)-induced renal fibrosis model, telocytes were injected via the tail vein every other day for 10 days...
April 28, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29710463/a-comprehensive-review-on-regulatory-effects-of-crocin-on-ischemia-reperfusion-injury-in-multiple-organs
#9
REVIEW
Ghaidafeh Akbari, Seyyed Ali Mard, Ali Veisi
Ischemia-reperfusion (I/R) injury affects o2 -dependent organs including liver, kidneys, heart, brain, and intestine. I/R injury is described as the cellular injury in an organ caused by ischemia and then further aggravated during the reperfusion due to intracellular alterations. It is a process that happens in clinical settings such as organ transplantation, reperfusion after thrombolytic therapy, and coronary angioplasty. Crocus sativus L. known as saffron used in folk medicine for its beneficial effects...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29695258/cells-responsible-for-liver-mass-regeneration-in-rats-with-2-acetylaminofluorene-partial-hepatectomy-injury
#10
Chin-Sung Chien, Ya-Hui Chen, Hui-Ling Chen, Chiu-Ping Wang, Shang-Hsin Wu, Shu-Li Ho, Wen-Cheng Huang, Chun-Hsien Yu, Mei-Hwei Chang
BACKGROUND: Whether hepatic progenitor cells (HPCs)/oval cells regenerate liver mass upon chronic liver injury is controversial in mice and has not been conclusively proven in humans and rats. In this study, we examined which cell type-hepatocytes or oval cells-mediates liver regeneration in the classic rat 2-acetylaminofluorene (AAF)/partial hepatectomy (PH) injury where AAF reversibly blocks hepatocyte proliferation, thereby inducing oval cell expansion after the regenerative stimulus of PH...
April 25, 2018: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29683437/re-arterialized-rat-partial-liver-transplantation-with-an-in-vivo-vessel-oriented-70-hepatectomy
#11
Xuehai Chen, Rong Yu, Ziqiang Xu, Yan Zhang, Chengyang Liu, Bicheng Chen, Hao Jin
Split liver transplantation and living liver donor liver transplantation were developed in the clinic to utilize liver organs in a more efficient manner. To better understand the mechanism behind these surgical procedures, a rat partial liver transplantation (PLTx) model was established for relevant surgical studies. Because of the complexity of the rat PLTx model, a protocol with detailed descriptions is required. An article published previously reported a protocol in which ex vivo hepatectomy was used to achieve 50% rat PLTx...
April 8, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29661451/ischemia-with-preconditioning-in-wistar-rats-maintains-mitochondrial-respiration-even-with-mild-hepatocellular-disturbance
#12
R R Tártaro, G L Jorge, A H Dianin, C A F Escanhoela, I F S F Boin
INTRODUCTION: In hepatectomy or liver transplantation, preconditioning is a procedure indicated to protect the organ from ischemia-reperfusion injury (I-R). OBJECTIVE: Evaluate the effect of preconditioning after hepatic I-R in Wistar rats, through mitochondrial respiration, liver histology, and profile. METHOD: Twenty male Wistar rats, weighing on average 307.1 g, were anesthetized with sodium thiopental (25 mg/kg) intravenously and xylazine hydrochloride (30 mg/kg) intramuscularly...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29661450/effect-of-hepatic-preconditioning-with-the-use-of-methylene-blue-on-the-liver-of-wistar-rats-submitted-to-ischemia-and-reperfusion
#13
T N de Almeida, J P Victorino, J Bistafa Liu, D Tófoli Queiroz Campos, C Graf, M C Jordani, L A Carneiro d'Albuquerque, K D S Mendes, O Castro-E-Silva
BACKGROUND: The liver may be injured in situations where it is submitted to ischemia, such as partial hepatectomy and liver transplantation. In all cases, ischemia is followed by reperfusion and, although it is essential for the reestablishment of tissue function, reperfusion may cause greater damage than ischemia, an injury characterized as ischemia-reperfusion (I/R) damage. The aim of this work was to analyze the effect of ischemic preconditioning with the use of methylene blue (MB; 15 mg/kg) 5 or 15 minutes before I/R (IRMB5' and IRMB15', respectively) on the hepatic injury occurring after I/R...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29617450/limb-remote-ischemic-conditioning-of-the-recipient-protects-the-liver-in-a-rat-model-of-arterialized-orthotopic-liver-transplantation
#14
Zoltan Czigany, Christian Bleilevens, Christian Beckers, Christian Stoppe, Michaela Möhring, Andras Fülöp, Attila Szijarto, Georg Lurje, Ulf P Neumann, René H Tolba
BACKGROUND: Ischemic-reperfusion (IR) injury still represents a major concern in clinical transplantation, especially in the era of extreme organ shortage and extended criteria donor organs. In the present study we aimed to investigate the hepatoprotective effects of remote ischemic conditioning (RIC) in a rat model of arterialized orthotopic liver transplantation (OLT). METHODS: Male Lewis rats were used (n = 144 / 72 OLT cases; 240-340g) as donors and recipients...
2018: PloS One
https://www.readbyqxmd.com/read/29608158/a-rat-model-of-orthotopic-liver-transplantation-using-a-novel-magnetic-anastomosis-technique-for-suprahepatic-vena-cava-reconstruction
#15
Lifei Yang, Jianwen Lu, Yue Wang, Mei Zhang, Yuan Shi, Shasha Wei, Peng Liu, Zheng Wu, Yi Lv, Rongqian Wu
The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep learning curve. The introduction of the cuff technique for anastomosis of the portal vein (PV) and infrahepatic vena cava (IHVC) has significantly simplified the transplant procedure in rats. However, due to the short anterior wall of the recipients' suprahepatic vena cava (SHVC), the cuff technique is very difficult to use for the reconstruction of the SHVC...
March 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29607608/three-dimensional-co-culture-of-primary-hepatocytes-and-stellate-cells-in-silk-scaffold-improves-hepatic-morphology-and-functionality-in-vitro
#16
Guofeng Wei, Jiwen Wang, Qiang Lv, Ming Liu, Hong Xu, He Zhang, Lingling Jin, Jiachuan Yu, Xiuli Wang
A vigorous in vitro model of liver that could recapitulate hepatic phenotype and functionality in vivo would exclusively improve the efficiency of bioartificial liver, drug discovery or even transplantation therapy. Owing to the indispensable role of 3D microenvironment in supporting viability and function of hepatocytes in vitro, much effort recently has been focused on improving reproducibility and standardization of primary hepatocyte cultures with a paradigm shift to 3D culture system, In the present study, an improved 3D co-culture system of hepatocytes was established in which rat primary hepatocytes were co-cultured with hepatic stellate cells in silk porous scaffolds...
April 1, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29593968/engineering-a-tumor-microenvironment-mimetic-niche-for-tissue-regeneration-with-xenogeneic-cancer-cells
#17
Zhenzhen Wang, Chunming Wang, Ayipaxia Abudukeremu, Xiaying Rui, Shang Liu, Xiaoyi Zhang, Min Zhang, Junfeng Zhang, Lei Dong
The insufficient number of cells suitable for transplantation is a long-standing problem to cell-based therapies aimed at tissue regeneration. Xenogeneic cancer cells (XCC) may be an alternative source of therapeutic cells, but their transplantation risks both immune rejection and unwanted spreading. In this study, a strategy to facilitate XCC transplantation is reported and their spreading in vivo is confined by constructing an engineering matrix that mimics the characteristics of tumor microenvironment. The data show that this matrix, a tumor homogenate-containing hydrogel (THAG), successfully creates an immunosuppressive enclave after transplantation into immunocompetent mice...
March 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/29570162/il-34-inhibits-acute-rejection-of-rat-liver-transplantation-by-inducing-kupffer-cell-m2-polarization
#18
Zhengfei Zhao, Guangrui Pan, Chengyong Tang, Zhongtang Li, Daofeng Zheng, Xufu Wei, Zhongjun Wu
BACKGROUND: Recent studies have demonstrated that IL-34 is implicated in the regulation of macrophage functions. However, the effect of IL-34 on Kupffer cells (KCs) in acute rejection (AR) of liver transplantation remains unclear. METHODS: IL-34 expression was detected in graft and serum from allotransplantation and syngeneic transplantation groups. The adeno-associated virus expressing IL-34 (AAV-IL-34) was used to assess the effect of IL-34 on AR of rat liver transplantation...
March 22, 2018: Transplantation
https://www.readbyqxmd.com/read/29562778/evidence-of-amniotic-epithelial-cell-differentiation-toward-hepatic-sinusoidal-endothelial-cells
#19
Monica Serra, Michela Marongiu, Antonella Contini, Toshio Miki, Erika Cadoni, Ezio Laconi, Fabio Marongiu
Amniotic epithelial cells (AECs) represent a useful and noncontroversial source for liver-based regenerative medicine, as they can differentiate into hepatocytes upon transplantation into the liver. However, the possibility that AECs can differentiate into other liver cell types, such as hepatic sinusoidal endothelial cells (HSECs), has never been assessed. In order to test this hypothesis, rat- and human-derived AECs (rAECs and hAECs, respectively) were subjected to endothelial cell tube formation assay in vitro...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29552139/transplantation-of-placenta-derived-multipotent-cells-in-rats-with-dimethylhydrazine-induced-colon-cancer-decreases-survival-rate
#20
Hanna Svitina, Inessa Skrypkina, Pavlo Areshkov, Vitaliy Kyryk, Tetiana Bukreieva, Pavlo Klymenko, Liudmyla Garmanchuk, Galyna Lobintseva, Volodymyr Shablii
Transplantation of placenta-derived multipotent cells (PDMCs) is a promising treatment method for many diseases. However, the impact of PDMCs on colon cancer has not yet been studied. PDMCs were obtained from rat placentas by culturing tissue explants. Colon cancer was experimentally induced in male albino Wistar rats by administering 20 mg/kg dimethylhydrazine (DMH) once a week for 20 consecutive weeks. The administration of the PDMCs was performed at the 20th week after the first DMH injection. The number and size of each tumour lesion were calculated in the 5th week after transplantation...
April 2018: Oncology Letters
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