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Liver transplantation in rat

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https://www.readbyqxmd.com/read/28109058/in-vivo-construction-of-liver-tissue-by-implantation-of-a-hepatic-non-parenchymal-adipose-derived-stem-cell-sheet
#1
Mio Fujii, Kosho Yamanouchi, Yusuke Sakai, Zhassulan Baimakhanov, Izumi Yamaguchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi
Subcutaneous hepatocyte sheet implantation is an attractive therapeutic option for various liver diseases. However, this technique is limited by the availability of hepatocytes. Thus, the use of hepatic non-parenchymal cells (NPCs) containing small hepatocytes, which have the ability to proliferate more rapidly than mature hepatocytes, for transplantation has been suggested. The aim of our study was to construct liver tissue subcutaneously in rats by implanting NPC sheets co-cultivated with adipose-derived stem cells (ADSCs), which produce certain angiogenic factors...
January 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28105981/cryopreservation-of-hepatocyte-microbeads-for-clinical-transplantation
#2
Suttiruk Jitraruch, Anil Dhawan, Robin D, Celine Filippi, Sharon C, Leanne Glover, Ragai R
Intraperitoneal transplantation of hepatocyte microbeads is an attractive option for the management of acute liver failure. Encapsulation of hepatocytes in alginate microbeads supports their function and prevents immune attack of the cells. Establishment of banked cryopreserved hepatocyte microbeads is important for emergency use. The aim of this study was to develop an optimised protocol for cryopreservation of hepatocyte microbeads for clinical transplantation using modified freezing solutions. Four freezing solutions with potential for clinical application were investigated...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28088007/impairment-of-host-liver-repopulation-by-transplanted-hepatocytes-in-aged-rats-and-the-release-by-short-term-growth-hormone-treatment
#3
Peggy Stock, Maximilian Bielohuby, Martin S Staege, Mei-Ju Hsu, Martin Bidlingmaier, Bruno Christ
Hepatocyte transplantation is an alternative to whole liver transplantation. Yet, efficient liver repopulation by transplanted hepatocytes is low in livers of old animals. This restraint might be because of the poor proliferative capacity of aged donor hepatocytes or the regenerative impairment of the recipient livers. The age-dependent liver repopulation by transplanted wild-type hepatocytes was investigated in juvenile and senescent rats deficient in dipeptidyl-peptidase IV. Repopulation was quantified by flow cytometry and histochemical estimation of dipeptidyl-peptidase IV enzyme activity of donor cells in the negative host liver...
January 11, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28077277/berberine-protects-against-ischemia-reperfusion-injury-after-orthotopic-liver-transplantation-via-activating-sirt1-foxo3%C3%AE-induced-autophagy
#4
Yuanbang Lin, Mingwei Sheng, Yiqi Weng, Rubin Xu, Ning Lu, Hongyin Du, Wenli Yu
The effects and mechanism of berberine (BBR) on hepatic injury after orthotopic liver transplantation (OLT) have not been well characterized. We examined the role of Sirt1/FoxO3α axis in the protective effect of BBR on ischemia/reperfusion injury after OLT. Adult male Wistar rats were randomly allocated into four groups: Sham, OLT, OLT with BBR pretreatment (BBR), OLT with BBR and Sirt1 inhibitor (EX527) pretreatment group (EX527). The liver function and oxidative stress level were measured by biochemical and histopathologic examinations...
January 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28069109/immunomodulatory-effects-of-bone-marrow-mesenchymal-stem-cells-overexpressing-heme-oxygenase-1-protective-effects-on-acute-rejection-following-reduced-size-liver-transplantation-in-a-rat-model
#5
Zhong-Yang Shen, Bin Wu, Tao Liu, Yang Yang, Ming-Li Yin, Wei-Ping Zheng, Bo-Ya Zhang, Hong-Li Song
Here we explore the T-lymphocyte suppressive and immunomodulatory effects of bone marrow mesenchymal stem cells (BMMSCs) overexpressing heme oxygenase-1 (HO-1) on acute rejection following reduced-size liver transplantation (RLT) in a rat model. The proliferation activity, cell cycle progression, secretion of proinflammatory cytokines, expression of CD25 and CD71 in lymphocytes, and activity of NK cells were found to be significantly lowered, and the proportion of regulatory T cells (Tregs) was found to be increased relative to BMMSCs when Adv-HO-1/BMMSCs were co-cultured with Con A ex vivo; secretion of anti-inflammatory cytokines was significantly higher...
December 26, 2016: Cellular Immunology
https://www.readbyqxmd.com/read/28009756/regeneration-and-cell-recruitment-in-an-improved-heterotopic-auxiliary-partial-liver-transplantation-model-in-the-rat
#6
Yoshihiro Ono, Angelica Pérez-Gutiérrez, Mladen I Yovchev, Kentaro Matsubara, Shinichiro Yokota, Jorge Guzman-Lepe, Kan Handa, Alexandra Collin de l'Hortet, Angus W Thomson, David A Geller, Hiroshi Yagi, Michael Oertel, Alejandro Soto-Gutierrez
BACKGROUND: Auxiliary partial liver transplantation (APLT) in humans is a therapeutic modality used especially to treat liver failure in children or congenital metabolic disease. Animal models of APLT have helped to explore therapeutic options. Though many groups have suggested improvements, standardizing the surgical procedure has been challenging. Additionally, the question of whether graft livers are reconstituted by recipient-derived cells after transplantation has been controversial...
January 2017: Transplantation
https://www.readbyqxmd.com/read/27998796/propofol-postconditioning-attenuates-hippocampus-ischemia-reperfusion-injury-via-modulating-jak2-stat3-pathway-in-rats-after-autogenous-orthotropic-liver-transplantation
#7
Jia Lili, Wang Fei, Gu Xiangqian, Weng Yiqi, Sheng Mingwei, Wang Gang, Li Shipeng, Du Hongyin, Yu Wenli
Liver transplantation has been a routine treatment for the end stage liver diseases. Severe changes in circulation system and internal environment may accur during transplant surgery and cause injury to many organs including brain. Specific mechanisms of brain injury associated with liver transplantation are not yet elucidated. Previous studies have shown that the JAK/STAT signal transduction pathways are involved in the development of the central nervous system, such as nerve cell proliferation, survival, differentiation, and it also have a role in the disease processes, including brain tumor, brain ischemia and other diseases of the central nervous system...
December 17, 2016: Brain Research
https://www.readbyqxmd.com/read/27960154/mesenchymal-stem-cells-with-enhanced-bcl-2-expression-promote-liver-recovery-in-a-rat-model-of-hepatic-cirrhosis
#8
Shizhu Jin, Hulun Li, Mingzi Han, Mengting Ruan, Zishuai Liu, Feifei Zhang, Chunwei Zhang, Yongsub Choi, Bingrong Liu
BACKGROUND/AIMS: Mesenchymal stem cell (MSC) transplantation has emerged as an option for the treatment of chronic hepatic cirrhosis, while its therapeutic efficacy could be improved. The bcl-2 gene is anti-apoptotic and can help cell survival and proliferation. Therefore, we explored whether transplanted MSCs with enhanced bcl-2 expression may be beneficial in the treatment of experimental cirrhosis in rats. METHODS: MSCs were isolated from rat bone marrow, expanded in vitro and transfected with adeno-associated virus (AAV) engineered the bcl-2 gene (AAV-bcl-2)...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27929382/aging-promotes-neoplastic-disease-through-effects-on-the-tissue-microenvironment
#9
Fabio Marongiu, Maria Paola Serra, Silvia Doratiotto, Marcella Sini, Maura Fanti, Erika Cadoni, Monica Serra, Ezio Laconi
A better understanding of the complex relationship between aging and cancer will provide important tools for the prevention and treatment of neoplasia. In these studies, the hypothesis was tested that aging may fuel carcinogenesis via alterations imposed in the tissue microenvironment. Preneoplastic hepatocytes isolated from liver nodules were orthotopically injected into either young or old syngeneic rats and their fate was followed over time using the dipeptidyl-peptidase type IV (DPPIV) system to track donor-derived-cells...
December 6, 2016: Aging
https://www.readbyqxmd.com/read/27925343/transplantation-of-thy1-cells-accelerates-liver-regeneration-by-enhancing-the-growth-of-small-hepatocyte-like-progenitor-cells-via-il17rb-signaling
#10
Norihisa Ichinohe, Masayuki Ishii, Naoki Tanimizu, Junko Kon, Yusuke Yoshioka, Takahiro Ochiya, Toru Mizuguchi, Koichi Hirata, Toshihiro Mitaka
Small hepatocyte-like progenitor cells (SHPCs) transiently form clusters in rat livers treated with retrorsine (Ret)/70% partial hepatectomy (PH). When Thy1(+) cells isolated from d-galactosamine-treated rat livers were transplanted into the livers of Ret/PH-treated rats, the mass of the recipient liver transiently increased during the first 30 days after transplantation, suggesting that liver regeneration was enhanced. Here we addressed how Thy1(+) cell transplantation stimulates liver regeneration. We found that the number and size of SHPC clusters increased in the liver at 14 days after transplantation...
December 7, 2016: Stem Cells
https://www.readbyqxmd.com/read/27909196/recommendation-for-severity-assessment-following-liver-resection-and-liver-transplantation-in-rats-part-i
#11
S Kanzler, A Rix, Z Czigany, H Tanaka, K Fukushima, B Kögel, K Pawlowsky, R H Tolba
Score sheets were first introduced 30 years ago to assess pain, distress and suffering in animals. To date, however, there is still no general agreement on their use in research practice, and only a few publications can be found on this topic. In the present work, we demonstrate the use of a special score sheet for severity assessment in the first three postoperative days in two showcased studies performed on Wistar and Lewis rats undergoing liver resection or orthotopic liver transplantation, respectively...
December 2016: Laboratory Animals
https://www.readbyqxmd.com/read/27894918/laser-speckle-contrast-imaging-and-oxygen-to-see-for-assessing-microcirculatory-liver-blood-flow-changes-following-different-volumes-of-hepatectomy
#12
Chong Hui Li, Xin Lan Ge, Ke Pan, Peng Fei Wang, Yi Nan Su, Ai Qun Zhang
OBJECTIVE: Portal hyperperfusion after extended hepatectomy or small-for-size liver transplantation may induce organ dysfunction and failure. This study was designed to monitor and characterize the hepatic microcirculatory perfusion following different volumes of hepatectomy in rats by using laser speckle contrast image (LSCI) and Oxygen to See (O2C), a spectrometric device. METHODS: The microcirculatory liver blood flow of the rats that underwent 68%, 85% and 90% hepatectomy (68PH, 85PH and 90PH) was monitored with LSCI and O2C before and following the hepatectomy...
November 25, 2016: Microvascular Research
https://www.readbyqxmd.com/read/27890868/serological-validation-of-an-alveolar-echinococcosis-rat-model-with-a-single-hepatic-lesion
#13
Masamichi Yamashita, Tomohiro Imagawa, Yasuhito Sako, Munehiro Okamoto, Tetsuya Yanagida, Yoshiharu Okamoto, Takeshi Tsuka, Tomohiro Osaki, Akira Ito
Serology is important for the diagnosis and follow-up of human alveolar echinococcosis (AE). However, patient conditions are highly variable among those with AE, and antibody responses in serological follow-up have not been well-defined. We recently described a new AE rat model established by implantation of small AE tissue into a single arbitrary location in the liver; no metastasis and dissemination were observed. In the present study, we examined the serological characteristics in our rat model before and after surgical treatment...
November 27, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27882463/the-use-of-technetium-99m-for-intravital-tracing-of-transplanted-multipotent-stromal-cells
#14
D N Silachev, A K Kondakov, I A Znamenskii, Yu B Kurashvili, A V Abolenskaya, N R Antipkin, T I Danilina, V N Manskikh, M V Gulyaev, Yu A Pirogov, E Yu Plotnikov, D B Zorov, G T Sukhikh
We studied the possibility of in vivo tracing of multipotent mesenchymal stromal cells labeled with a radiophermaceutic preparation based on metastable isotope Technetium-99m and injected to rats with modeled traumatic brain injury. Accumulation of labeled cells occurred primarily in the liver and lungs. The cells distribution in internal organs greatly varied depending on the administration route. Cell injection into the carotid artery led to their significant accumulation in the damaged brain hemisphere, while intravenous injection was followed by diffuse cell distribution in all brain structures...
November 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27882135/protective-effect-of-enos-overexpression-against-ischemia-reperfusion-injury-in-small-for-size-liver-transplantation
#15
Bo Zhang, Qiu-Hua Liu, Cui-Jie Zhou, Ming-Zheng Hu, Hai-Xin Qian
Ischemia/reperfusion (I/R) injury can occur during small-for-size liver transplantation, resulting in delayed graft function and decreased long-term graft survival. The aim of the present study was to evaluate the effects of genetic overexpression of endothelial nitric oxide synthase (eNOS) in protecting hepatocytes against I/R injury in a rat model of small-for-size liver transplantation. L02 liver cells were transfected with the eNOS gene using an adenovirus (Ad-eNOS). eNOS expression was detected using quantitative polymerase chain reaction and western blot analysis...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27861845/quantum-dots-labelling-allows-detection-of-the-homing-of-mesenchymal-stem-cells-administered-as-immunomodulatory-therapy-in-an-experimental-model-of-pancreatic-islets-transplantation
#16
Silvia Mannucci, Laura Calderan, Paola Quaranta, Sara Antonini, Franco Mosca, Biancamaria Longoni, Pasquina Marzola, Federico Boschi
Cell transplantation is considered a promising therapeutic approach in several pathologies but still needs innovative and non-invasive imaging technologies to be validated. The use of mesenchymal stem cells (MSCs) attracts major interest in clinical transplantation thanks to their regenerative properties, low immunogenicity and ability to regulate immune responses. In several animal models, MSCs are used in co-transplantation with pancreatic islets (PIs) for the treatment of type I diabetes, supporting graft survival and prolonging normal glycaemia levels...
November 15, 2016: Journal of Anatomy
https://www.readbyqxmd.com/read/27860296/impact-of-subnormothermic-machine-perfusion-preservation-in-severely-steatotic-rat-livers-a-detailed-assessment-in-an-isolated-setting
#17
Y Okamura, K Hata, H Tanaka, H Hirao, T Kubota, O Inamoto, S Kageyama, I Tamaki, N Yermek, J Yoshikawa, S Uemoto
The current drastic shortage of donor organs has led to acceptance of extended-criteria donors for transplantation, despite higher risk of primary nonfunction. Here, we report the impact of subnormothermic machine perfusion (SMP) preservation on the protection of >50% macrosteatotic livers. Dietary hepatic steatosis was induced in Wistar rats via 2-day fasting and subsequent 3-day re-feeding with a fat-free, carbohydrate-rich diet. This protocol induces 50-60% macrovesicular steatosis, which should be discarded when preserved via cold storage (CS)...
November 10, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27848069/adenosine-receptors-regulatory-players-in-the-preservation-of-mitochondrial-function-induced-by-ischemic-preconditioning-of-rat-liver
#18
Filipe V Duarte, João A Amorim, Ana T Varela, João S Teodoro, Ana P Gomes, Rodrigo A Cunha, Carlos M Palmeira, Anabela P Rolo
Although adenosine A1 receptors (A1R) have been associated to ischemic preconditioning (IPC), direct evidence for their ability to preserve mitochondrial function upon hepatic preconditioning is still missing and could represent a novel strategy to boost the quality of liver transplants. We tested if the A1R antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) prevented IPC in the liver and if the A1R agonist 2-chloro-N(6)-cyclopentyladenosine (CCPA) might afford a pharmacological preconditioning. Livers underwent a 120 min of 70% warm ischemia and 16 h of reperfusion (I/R), and the IPC group underwent a 5-min ischemic episode followed by a 10-min period of reperfusion before I/R...
November 15, 2016: Purinergic Signalling
https://www.readbyqxmd.com/read/27830556/deep-digging-far-red-imaging-for-the-monitoring-of-transplanted-hepatocytes-in-rats
#19
Peggy Stock, Kristin Weber
Technologies for in vivo imaging of the distribution and integration of cell transplants gain significance for the use of novel cell therapy approaches in regenerative medicine. Applied to adequate animal models, they provide information on the spatio-temporal engraftment and functional performance of the cells transplanted. This chapter includes a detailed description of the in vivo tracking of transplanted hepatocytes in rat liver including the conjugation of antibodies to fluorochromes for far red imaging using a multispectral optical imager...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830554/minimally-invasive-liver-preconditioning-for-hepatocyte-transplantation-in-rats
#20
Martin Gaillard, Ibrahim Dagher
In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.
2017: Methods in Molecular Biology
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