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Liver transplantation in rat

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https://www.readbyqxmd.com/read/27925343/transplantation-of-thy1-cells-accelerates-liver-regeneration-by-enhancing-the-growth-of-small-hepatocyte-like-progenitor-cells-via-il17rb-signaling
#1
Norihisa Ichinohe, Masayuki Ishii, Naoki Tanimizu, Junko Kon, Yusuke Yoshioka, Takahiro Ochiya, Toru Mizuguchi, Koichi Hirata, Toshihiro Mitaka
Small hepatocyte-like progenitor cells (SHPCs) transiently form clusters in rat livers treated with retrorsine (Ret)/70% partial hepatectomy (PH). When Thy1(+) cells isolated from d-galactosamine-treated rat livers were transplanted into the livers of Ret/PH-treated rats, the mass of the recipient liver transiently increased during the first 30 days after transplantation, suggesting that liver regeneration was enhanced. Here we addressed how Thy1(+) cell transplantation stimulates liver regeneration. We found that the number and size of SHPC clusters increased in the liver at 14 days after transplantation...
December 7, 2016: Stem Cells
https://www.readbyqxmd.com/read/27909196/recommendation-for-severity-assessment-following-liver-resection-and-liver-transplantation-in-rats-part-i
#2
S Kanzler, A Rix, Z Czigany, H Tanaka, K Fukushima, B Kögel, K Pawlowsky, R H Tolba
Score sheets were first introduced 30 years ago to assess pain, distress and suffering in animals. To date, however, there is still no general agreement on their use in research practice, and only a few publications can be found on this topic. In the present work, we demonstrate the use of a special score sheet for severity assessment in the first three postoperative days in two showcased studies performed on Wistar and Lewis rats undergoing liver resection or orthotopic liver transplantation, respectively...
December 2016: Laboratory Animals
https://www.readbyqxmd.com/read/27894918/laser-speckle-contrast-imaging-and-oxygen-to-see-for-assessing-microcirculatory-liver-blood-flow-changes-following-different-volumes-of-hepatectomy
#3
Chong Hui Li, Xin Lan Ge, Ke Pan, Peng Fei Wang, Yi Nan Su, Ai Qun Zhang
OBJECTIVE: Portal hyperperfusion after extended hepatectomy or small-for-size liver transplantation may induce organ dysfunction and failure. This study was designed to monitor and characterize the hepatic microcirculatory perfusion following different volumes of hepatectomy in rats by using laser speckle contrast image (LSCI) and Oxygen to See (O2C), a spectrometric device. METHODS: The microcirculatory liver blood flow of the rats that underwent 68%, 85% and 90% hepatectomy (68PH, 85PH and 90PH) was monitored with LSCI and O2C before and following the hepatectomy...
November 25, 2016: Microvascular Research
https://www.readbyqxmd.com/read/27890868/serological-validation-of-an-alveolar-echinococcosis-rat-model-with-a-single-hepatic-lesion
#4
Masamichi Yamashita, Tomohiro Imagawa, Yasuhito Sako, Munehiro Okamoto, Tetsuya Yanagida, Yoshiharu Okamoto, Takeshi Tsuka, Tomohiro Osaki, Akira Ito
Serology is important for the diagnosis and follow-up of human alveolar echinococcosis (AE). However, patient conditions are highly variable among those with AE, and antibody responses in serological follow-up have not been well-defined. We recently described a new AE rat model established by implantation of small AE tissue into a single arbitrary location in the liver; no metastasis and dissemination were observed. In the present study, we examined the serological characteristics in our rat model before and after surgical treatment...
November 27, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27882463/the-use-of-technetium-99m-for-intravital-tracing-of-transplanted-multipotent-stromal-cells
#5
D N Silachev, A K Kondakov, I A Znamenskii, Yu B Kurashvili, A V Abolenskaya, N R Antipkin, T I Danilina, V N Manskikh, M V Gulyaev, Yu A Pirogov, E Yu Plotnikov, D B Zorov, G T Sukhikh
We studied the possibility of in vivo tracing of multipotent mesenchymal stromal cells labeled with a radiophermaceutic preparation based on metastable isotope Technetium-99m and injected to rats with modeled traumatic brain injury. Accumulation of labeled cells occurred primarily in the liver and lungs. The cells distribution in internal organs greatly varied depending on the administration route. Cell injection into the carotid artery led to their significant accumulation in the damaged brain hemisphere, while intravenous injection was followed by diffuse cell distribution in all brain structures...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27882135/protective-effect-of-enos-overexpression-against-ischemia-reperfusion-injury-in-small-for-size-liver-transplantation
#6
Bo Zhang, Qiu-Hua Liu, Cui-Jie Zhou, Ming-Zheng Hu, Hai-Xin Qian
Ischemia/reperfusion (I/R) injury can occur during small-for-size liver transplantation, resulting in delayed graft function and decreased long-term graft survival. The aim of the present study was to evaluate the effects of genetic overexpression of endothelial nitric oxide synthase (eNOS) in protecting hepatocytes against I/R injury in a rat model of small-for-size liver transplantation. L02 liver cells were transfected with the eNOS gene using an adenovirus (Ad-eNOS). eNOS expression was detected using quantitative polymerase chain reaction and western blot analysis...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27861845/quantum-dots-labelling-allows-detection-of-the-homing-of-mesenchymal-stem-cells-administered-as-immunomodulatory-therapy-in-an-experimental-model-of-pancreatic-islets-transplantation
#7
Silvia Mannucci, Laura Calderan, Paola Quaranta, Sara Antonini, Franco Mosca, Biancamaria Longoni, Pasquina Marzola, Federico Boschi
Cell transplantation is considered a promising therapeutic approach in several pathologies but still needs innovative and non-invasive imaging technologies to be validated. The use of mesenchymal stem cells (MSCs) attracts major interest in clinical transplantation thanks to their regenerative properties, low immunogenicity and ability to regulate immune responses. In several animal models, MSCs are used in co-transplantation with pancreatic islets (PIs) for the treatment of type I diabetes, supporting graft survival and prolonging normal glycaemia levels...
November 15, 2016: Journal of Anatomy
https://www.readbyqxmd.com/read/27860296/impact-of-subnormothermic-machine-perfusion-preservation-in-severely-steatotic-rat-livers-a-detailed-assessment-in-an-isolated-setting
#8
Y Okamura, K Hata, H Tanaka, H Hirao, T Kubota, O Inamoto, S Kageyama, I Tamaki, N Yermek, J Yoshikawa, S Uemoto
Current drastic shortage of donor organs has led to acceptance of extended-criteria donors for transplantation, despite higher risk of primary non-function. Here, we report the impact of subnormothermic machine perfusion (SMP) preservation on the protection of >50% macro-steatotic livers. Dietary hepatic steatosis was induced in Wistar rats via 2-day fasting and subsequent 3-day re-feeding with a fat-free, carbohydrate-rich diet. This protocol induces 50-60% macrovesicular steatosis, which should be discarded when preserved via cold storage (CS)...
November 10, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27848069/adenosine-receptors-regulatory-players-in-the-preservation-of-mitochondrial-function-induced-by-ischemic-preconditioning-of-rat-liver
#9
Filipe V Duarte, João A Amorim, Ana T Varela, João S Teodoro, Ana P Gomes, Rodrigo A Cunha, Carlos M Palmeira, Anabela P Rolo
Although adenosine A1 receptors (A1R) have been associated to ischemic preconditioning (IPC), direct evidence for their ability to preserve mitochondrial function upon hepatic preconditioning is still missing and could represent a novel strategy to boost the quality of liver transplants. We tested if the A1R antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) prevented IPC in the liver and if the A1R agonist 2-chloro-N(6)-cyclopentyladenosine (CCPA) might afford a pharmacological preconditioning. Livers underwent a 120 min of 70% warm ischemia and 16 h of reperfusion (I/R), and the IPC group underwent a 5-min ischemic episode followed by a 10-min period of reperfusion before I/R...
November 15, 2016: Purinergic Signalling
https://www.readbyqxmd.com/read/27830556/deep-digging-far-red-imaging-for-the-monitoring-of-transplanted-hepatocytes-in-rats
#10
Peggy Stock, Kristin Weber
Technologies for in vivo imaging of the distribution and integration of cell transplants gain significance for the use of novel cell therapy approaches in regenerative medicine. Applied to adequate animal models, they provide information on the spatio-temporal engraftment and functional performance of the cells transplanted. This chapter includes a detailed description of the in vivo tracking of transplanted hepatocytes in rat liver including the conjugation of antibodies to fluorochromes for far red imaging using a multispectral optical imager...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830554/minimally-invasive-liver-preconditioning-for-hepatocyte-transplantation-in-rats
#11
Martin Gaillard, Ibrahim Dagher
In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830552/demonstrating-potential-of-cell-therapy-for-wilson-s-disease-with-the-long-evans-cinnamon-rat-model
#12
Fadi Luc Jaber, Yogeshwar Sharma, Sanjeev Gupta
Wilson's disease (WD) is characterized by the inability to excrete copper (Cu) from the body with progressive tissue injury, especially in liver and brain. The molecular defect in WD concerns mutations in ATP7B gene leading to loss of Cu transport from the hepatocyte to the bile canaliculus. While drugs, e.g., Cu chelators, have been available for several decades, these must be taken lifelong, which can be difficult due to issues of compliance or side effects. Many individuals may require liver transplantation, which can also be difficult due to donor organ shortages...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830550/gunn-rats-as-a-surrogate-model-for-evaluation-of-hepatocyte-transplantation-based-therapies-of-crigler-najjar-syndrome-type-1
#13
Zsuzsanna Polgar, Yanfeng Li, Xia Li Wang, Chandan Guha, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
Liver transplantation has been established as a curative therapy for acute and chronic liver failure, as well as liver-based inherited metabolic diseases. Because of the complexity of organ transplantation and the worldwide shortage of donor organs, hepatocyte transplantation is being developed as a bridging therapy until donor organs become available, or for amelioration of inherited liver-based diseases. The Gunn rat is a molecular and metabolic model of Crigler-Najjar syndrome type 1, which is characterized by lifelong unconjugated hyperbilirubinemia due to the lack of uridinediphosphoglucuronate glucuronosyltransferase-1 (UGT1A1)-mediated bilirubin glucuronidation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830548/fetal-liver-stem-progenitor-cell-transplantation-a-model-to-study-tissue-mass-replacement-and-cell-based-therapies
#14
Mladen I Yovchev, Michael Oertel
Liver transplantation is the only therapeutic treatment for patients with end-stage liver diseases. However, donor organ scarcity is the major limitation, and therefore, alternative strategies are urgently needed. The ultimate goal for successful cell-based therapies is the ability of transplanted cells to efficiently engraft and reconstitute injured liver mass. To evaluate the repopulation capacity of transplanted cells, it is essential to identify their specific characteristics, as well as to study the mechanism(s) Through which transplanted donor cells replace tissue mass in hepatic microenvironments, using well-established cell transplantation models...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830547/propagation-of-human-hepatocytes-in-upa-scid-mice-producing-chimeric-mice-with-humanized-liver
#15
Hiroki Ohshita, Chise Tateno
Primary or cryopreserved human hepatocytes (h-heps) have been used as the gold standard for in vitro metabolism and hepatotoxicity studies; however, the supply of h-heps is limited and they cannot grow in vitro. We achieved approximately 1000-fold propagation of h-heps in the liver of albumin promoter/enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency disease (uPA/SCID) mice with genetically induced liver disease and immunodeficiency. When h-heps are transplanted into the uPA/SCID mouse liver via the spleen, the h-heps engraft in the mouse liver, resulting in its repopulation with h-heps...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27830544/late-gestation-fetal-hepatocytes-for-liver-repopulation-in-the-rat
#16
Jennifer A Sanders
Cellular transplantation represents an alternative to liver transplantation for the treatment of end-stage liver disease and liver-based inborn errors of metabolism. In order for cellular transplantation to be successful, an optimal source of cells for transplantation needs to be identified and the molecular mechanisms regulating their engraftment, proliferation, and functional differentiation elucidated. Here we describe a detailed protocol for the isolation, selection, and transplantation into an injured adult rat liver of a defined population of late gestation fetal rat hepatocytes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27788823/isoflurane-an-ideal-anesthetic-for-rodent-orthotopic-liver-transplantation-surgery
#17
D Cao, Y Liu, J Li, J Gong
PURPOSE: Because the choice of anesthetic affects the rodent orthotopic liver transplantation (OLT) model, we compared the effects of isoflurane, ketamine, chloral hydrate, and pentobarbital on the OLT model. BASIC PROCEDURES: OLT was performed using the two-cuff technique. Two hundred male rats were randomly divided into five groups: control, isoflurane, ketamine, chloral hydrate, and pentobarbital groups. Rectal temperatures, respiratory rates, arterial blood values (pH, PaCO2, PaO2, and SatO2), liver function tests and histopathology, recovery times, and anhepatic stage mortality rates were assessed...
October 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27788821/irak-4-shrna-prevents-ischemia-reperfusion-injury-via-different-perfusion-periods-through-the-portal-vein-after-liver-transplantation-in-rat
#18
Y Wu, Y Liu, M Li, Z Liu, J Gong
BACKGROUND: This study analyzed the effects of short hairpin RNA targeting interleukin-1 receptor-associated kinase-4 (IRAK-4-shRNA) via portal vein perfusion during different periods on ischemia/reperfusion injury after liver transplantation. METHODS: Rats were randomly divided into 3 groups: the cold ischemia transfection group (CIT group, n = 18), in which graft livers were perfused with the plasmid of pSIIRAK-4 expressing IRAK-4-shRNA for 4 minutes (0.5 mL/min) via the portal vein during the cold ischemia period; the in vivo transfection group (IVT group, n = 18), in which equivalent volumes (2 mL) of IRAK-4-ShRNA plasmid (pSIIRAK-4) were injected during the operation; and the control group (n = 18), in which the rats received equivalent volumes of blank plasmid...
October 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27788708/-role-of-neutrophils-in-treatment-of-rats-with-d-galactosamine-induced-acute-liver-failure-with-bone-marrow-mesenchymal-stem-cells
#19
X Zhao, X L Shi, Z H Zhang, H C Ma, X W Yuan, Y T Ding
Objective: To investigate the therapeutic effect of bone marrow mesenchymal stromal cell (BMSC) transplantation on D-galactosamine-induced acute liver failure in Sprague-Dawley (SD) rats, as well as the mechanism of neutrophils in this process. Methods: A total of 39 male SD rats were divided into control group (8 rats, intraperitoneal injection of isotonic saline), model group (10 rats, intraperitoneal injection of D-galactosamine), solvent group (9 rats, tail vein injection of isotonic saline at 2 hours after intraperitoneal injection of D-galactosamine), and treatment group (12 rats, tail vein injection of MSCs at 2 hours after intraperitoneal injection of D-galactosamine)...
August 20, 2016: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/27785340/comparison-of-the-therapeutic-effectiveness-of-human-cd34-and-rat-bone-marrow-mesenchymal-stem-cells-on-improvement-of-experimental-liver-fibrosis-in-wistar-rats
#20
Hayam G Sayyed, Amany Osama, Naglaa K Idriss, Dina Sabry, Azza S Abdelrhim, Rania Bakry
BACKGROUND AND OBJECTIVE: Human umbilical cord blood (UCB) cells and bone marrow mesenchymal stem cells (BM-MSCs) have numerous advantages as grafts for cell transplantation. We hypothesized differing impacts of human UCB cells and rat BM-MSCs on reversal of hepatic injury and revival of liver function in carbon tetrachloride (CCl4)-induced liver fibrosis. METHODS: Forty rats were divided into 4 groups; control group, CCl4 group, CCl4/CD34(+) group and CCl4/BM-MSCs group...
2016: International Journal of Physiology, Pathophysiology and Pharmacology
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