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Liver transplantation in rat

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https://www.readbyqxmd.com/read/29344306/131i-labeled-copper-sulfide-loaded-microspheres-to-treat-hepatic-tumors-via-hepatic-artery-embolization
#1
Qiufang Liu, Yuyi Qian, Panli Li, Sihang Zhang, Jianjun Liu, Xiaoguang Sun, Michael Fulham, Dagan Feng, Gang Huang, Wei Lu, Shaoli Song
Purpose: Transcatheter hepatic artery embolization therapy is a minimally invasive alternative for treating inoperable liver cancer but recurrence is frequent. Multifunctional agents, however, offer an opportunity for tumor eradication. In this study, we were aim to synthesized poly (lactic-co-glycolic acid) (PLGA) microspheres encapsulating hollow CuS nanoparticles (HCuSNPs) and paclitaxel (PTX) that were then labeled with radioiodine-131 (131I) to produce 131I-HCuSNPs-MS-PTX. This compound combines the multi-theranostic properties of chemotherapy, radiotherapy and photothermal therapy...
2018: Theranostics
https://www.readbyqxmd.com/read/29332347/genetically-engineered-bone-marrow-derived-mesenchymal-stem-cells-co-expressing-ifn-%C3%AE-and-il-10-inhibit-hepatocellular-carcinoma-by-modulating-mapk-pathway
#2
Hao Wang, Jun Wang, Xiaolei Shi, Yitao Ding
PURPOSE: One of the major challenges in delivering cytokines for the treatment of hepatocellular carcinoma (HCC) is the mode of delivery. This study hypothesized that genetically engineered bone marrow derived mesenchymal stem cells (BMSCs) co-expressing IFN-γ and IL-10 can serve as a potential therapeutic strategy in the treatment of HCC by inhibiting cell proliferation. METHODS: Male Sprague-Dawley rats (n=5, 200-250 g) for BMSCs isolation and Nude/SCID mice (n=35,12-20g) to develop liver cancer xenograft model were used...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29319169/cell-tracking-survival-and-differentiation-capacity-of-adipose-derived-stem-cells-after-engraftment-in-rat-tissue
#3
Mario F Muñoz, Sandro Argüelles, Matias Guzman-Chozas, Remedios Guillén-Sanz, Jaime M Franco, José A Pintor-Toro, Mercedes Cano, Antonio Ayala
Adipose tissue is an important source of adipose derived stem cells (ADSCs). These cells have the potential of being used for certain therapies, in which the main objective is to recover the function of a tissue/organ affected by a disease. In order to contribute to repair of the tissue, these cells should be able to survive and carry out their functions in unfavorable conditions after being transplanted. This process requires a better understanding of the biology involved: such as the time cells remain in the implant site, how long they stay there, and whether or not they differentiate into host tissue cells...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29312561/therapeutic-effects-of-adipose-derived-mesenchymal-stem-cells-against-brain-death-induced-remote-organ-damage-and-post-heart-transplant-acute-rejection
#4
Hon-Kan Yip, Mel S Lee, Cheuk-Kwan Sun, Kuan-Hung Chen, Han-Tan Chai, Pei-Hsun Sung, Kun-Chen Lin, Sheung-Fat Ko, Chun-Man Yuen, Chu-Feng Liu, Pei-Lin Shao, Fan-Yen Lee
We tested the hypothesis that allogenic adipose-derived mesenchymal stem cells (ADMSCs) alleviated brain death (BD)-induced remote organ damage and events of post heart-transplant acute rejection. To determine the impact of BD on remote organ damage, adult-male F344 rats (n=24) were categorized sham-control (SC), BD and BDMSC (allogenic ADMSC/1.2 × 106 cells/derived from F344 by intravenous transfusion 3 h after BD procedure). To determine the protective effect of allogenic ADMSCs, animals (n=8/each group in F344/Lewis) were categorized into groups BD-T(F344 heart transplanted into Lewis by 6h after BD), BD-TMSC(D1/3) (BD induction for 6h then heart transplantation, and allogenic ADMSCs transfusion at days 1 and 5 after heart transplantation), BD-TMSC(3h) (BD + ADMSC/1...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29290803/glutathione-peroxidase-3-delivered-by-hipsc-mscs-ameliorated-hepatic-ir-injury-via-inhibition-of-hepatic-senescence
#5
Xiang Qi, Kevin Tak-Pan Ng, Qizhou Lian, Chang Xian Li, Wei Geng, Chang Chun Ling, Wai Ho Yeung, Yuen Yuen Ma, Xiao Bing Liu, Hui Liu, Jiang Liu, Xin Xiang Yang, Chung Mau Lo, Kwan Man
Background and Aims: Down-regulation of GPx3 accelerated hepatic senescence, which further caused overwhelming inflammation and severe liver graft injury. MSCs derived from human induced pluripotent stem cells (hiPSC-MSCs) have been developed as more efficient delivery vehicle with the property of injury tropism. Here, we aimed to explore the suppressive role of GPx3 in hepatic IR injury using novel delivery system of hiPSC-MSCs. Methods: The mice IR injury model with partial hepatectomy was established. The engineered hiPSC-MSCs delivering GPx3 was constructed...
2018: Theranostics
https://www.readbyqxmd.com/read/29290652/in-vivo-hepatic-differentiation-potential-of-human-umbilical-cord-derived-mesenchymal-stem-cells-therapeutic-effect-on-liver-fibrosis-cirrhosis
#6
Guo-Zun Zhang, Hui-Cong Sun, Li-Bo Zheng, Jin-Bo Guo, Xiao-Lan Zhang
AIM: To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis. METHODS: A CCl4-induced liver fibrotic/cirrhotic rat model was used to assess the effect of hUC-MSCs. Histopathology was assessed by hematoxylin and eosin (H&E), Masson trichrome and Sirius red staining. The liver biochemical profile was measured using a Beckman Coulter analyzer...
December 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29286833/liver-x-receptors-activation-attenuates-ischemia-reperfusion-injury-of-liver-graft-in-rats
#7
Ming-Xiang Cheng, Ping Huang, Qiang He, Yong Chen, Jin-Zheng Li
PURPOSE: Suppression of the Toll like receptor 4 (TLR4)-nuclear factor-κB (NF-κB) signaling was critical in protection against liver IRI. Previous studies revealed that Liver X receptors (LXRs) activation could antagonize TLR4-NF-κB signaling. The purpose of this study is to determine whether LXRs agonist GW3965 can suppress the TLR4-NF-κB signaling during liver transplantation and protect ischemia-reperfusion injury (IRI). MATERIALS AND METHODS: Sprague Dawley (SD) rats were used to perform orthotropic liver transplantation...
December 29, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/29279846/autoantibodies-against-cyp-2c19-a-novel-serum-marker-in-pediatric-de-novo-autoimmune-hepatitis
#8
Maria Grazia Clemente, Roberto Antonucci, Claudia Mandato, Lucia Cicotto, Antonella Meloni, Bruno Gridelli, Stefano De Virgiliis, Michael P Manns, Pietro Vajro
Diagnosis of de novo autoimmune hepatitis (AIH) after orthotopic liver transplantation (OLT) is challenging especially in the absence of hyper-γ-globulinemia. Circulating autoantibodies are not sensitive nor specific in de novo AIH but when positive increase the diagnostic probability. We report the discovery of novel liver microsomal (LM) autoantibodies against CYP-2C19 in a 9-year-old boy with "de novo" AIH developed 7 years after OLT. Graft dysfunction presented with hypertransaminasemia (up to 400 IU/L), while serum γ-globulins remained within the normal range for age...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29259704/effect-of-human-umbilical-cord-blood-stem-cell-transplantation-on-oval-cell-response-in-2-aaf-ccl4-liver-injury-model-experimental-immunohistochemical-study
#9
Hussein Abdellatif, Gamal Shiha, Dalia M Saleh, Huda Eltahry, Kamal G Botros
Background: Oval cells, specific liver progenitors, are activated in response to injury. The human umbilical cord blood (hUCB) is a possible source of transplantable hepatic progenitors and can be used in cases of severe liver injury. We detected the effect of hUCB stem cell transplantation on natural response of oval cells to injury. Methods: Twenty-four female albino rats were randomly divided into three groups: (A) control, (B) liver injury with hepatocyte block, and (C) hUCB transplanted group...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29258603/adipose-tissue-derived-stem-cells-ameliorate-hyperglycemia-insulin-resistance-and-liver-fibrosis-in-the-type-2-diabetic-rats
#10
Naishun Liao, Youshi Zheng, Haihua Xie, Bixing Zhao, Yongyi Zeng, Xiaolong Liu, Jingfeng Liu
BACKGROUND: Type 2 diabetes (T2D) is closely associated with liver fibrosis, but no effective treatments are currently available. This study was designed to investigate the therapeutic effects of ADSCs on insulin resistance, hyperglycemia, and liver fibrosis on T2D rats. METHODS: We first established a T2D rat model with liver fibrosis by using the combination of a high-fat diet (HFD), low-dose streptozotocin (STZ), and carbon tetrachloride (CCl4). Subsequently, the model rats were administrated by tail vein injection of PBS or ADSCs, respectively...
December 19, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29250120/human-chorionic-plate-derived-mesenchymal-stem-cells-restore-hepatic-lipid-metabolism-in-a-rat-model-of-bile-duct-ligation
#11
Yun Bin Lee, Jong Ho Choi, Eun Nam Kim, Jin Seok, Hyun-Jung Lee, Jung-Hwan Yoon, Gi Jin Kim
In cholestatic liver diseases, impaired bile excretion disrupts lipid homeostasis. We investigated changes of lipid metabolism, including mitochondrial β-oxidation, in a rat model of bile duct ligation (BDL) in which chorionic plate-derived mesenchymal stem cells (CP-MSCs) were transplanted. Serum cholesterol level, which was elevated after BDL, was significantly decreased following CP-MSC transplantation. The expression levels of genes involved in intracellular lipid uptake, including long-chain fatty acyl-CoA synthetases and fatty acid transport proteins, were decreased in rats after BDL; however, they were not significantly changed by subsequent CP-MSC transplantation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29237388/crigler-najjar-syndrome-current-perspectives-and-the-application-of-clinical-genetics
#12
Ammar Ebrahimi, Fakher Rahim
Crigler-Najjar syndrome (CNS, OMIM: 218800) is the paradigm of an inborn error of metabolism and a rare genetic disease with an estimated incidence of 0.6-1.0 per million live births. Discrimination between CNS subtypes is usually done on the basis of the clinical criteria, such as response to phenobarbital treatment and other molecular and functional characteristics. The identification of four novel pathogenic mutations and the analysis of residual activity of missense in UGT1A1 gene are useful for clinical diagnosis, and may reveal a new insight in enzyme activity, whereas the identification of pathogenic mutations will accelerate genetic counseling for newly identified CNS patients...
December 13, 2017: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/29228730/wisp2-disruption-represses-cxcr4-expression-and-inhibits-bmscs-homing-to-injured-liver
#13
Dan Qin, Yi Yan, Bian Hu, Wanpo Zhang, Hanmin Li, Xiaodong Li, Shenghui Liu, Depeng Dai, Xiongji Hu, Xingxu Huang, Lisheng Zhang
Liver regeneration/repair is a compensatory regrowth following acute liver failure, and bone marrow-derived mesenchyme stem cell (BMSC) transplantation is an effective therapy that promotes liver regeneration/repair. Wnt1 inducible signaling pathway protein 2 (Wisp2) is highly expressed in BMSCs, however, its function remains unclear. In this work, we used clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein -9 nuclease (CRISPR/Cas9) genome editing technology to knockdown Wisp2 in BMSCs, and these modified cells were then transplanted into rats which were induced by the 2-AAF/PH...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29218087/the-cytoskeleton-protein-%C3%AE-actin-may-mediate-t-cell-apoptosis-during-acute-rejection-reaction-after-liver-transplantation-in-a-rat-model
#14
Xiaolong Chen, Jun Zheng, Jianye Cai, Hui Li, Shihui Li, Li Wang, Daorou Cheng, Huaxin Chen, Yang Yang, Guihua Chen, Qi Zhang, Yanwen Peng, Qiyou Wang, Genshu Wang
Cytoskeletal proteins and associated regulatory proteins are essential for maintaining cell structure and growth. β-actin is a major component of the cytoskeleton, and β-actin remodeling is involved in lymphocyte migration, infiltration and apoptosis. However, little is known about whether changes in β-actin expression affect lymphocyte cell fate, particularly during acute rejection after liver transplantation in a rat model. In our studies, grafts were harvested on days 5, 7 or 9 after xenogeneic rat liver transplantation...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29216568/nigella-sativa-oil-modulates-the-therapeutic-efficacy-of-mesenchymal-stem-cells-against-liver-injury-in-irradiated-rats
#15
Rasha R Radwan, Heba A Mohamed
Stem cell transplantation is a novel strategy for regenerative medicine in liver disease. This study was conducted to explore the modulatory effect of Nigella sativa oil (NSO) on the therapeutic potential of mesenchymal stem cells (MSCs) against irradiation-induced liver damage in rats. Liver damage was induced by a total body exposure to a single dose of 7Gy. NSO (2mg/kg/day) was then given orally for 4 consecutive weeks starting 24h after irradiation with or without a single intravenous MSCs administration, then rats were sacrificed four weeks after exposure to γ radiation...
December 2, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29211918/decellularized-human-placenta-supports-hepatic-tissue-and-allows-rescue-in-acute-liver-failure
#16
Zurab Kakabadze, Anne Kakabadze, David Chakhunashvili, Lika Karalashvili, Ekaterine Berishvili, Yogeshwar Sharma, Sanjeev Gupta
Tissue engineering with scaffolds to form transplantable organs is of wide interest. Decellularized tissues have been tested for this purpose, although supplies of healthy donor tissues, vascular recellularization for perfusion and tissue homeostasis in engineered organs pose challenges. We hypothesized decellularized human placenta will be suitable for tissue engineering. The universal availability and unique structures of placenta for accommodating tissue, including presence of embedded vessels, were major attractions...
December 6, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29187911/oval-cells-contribute-to-fibrogenesis-of-marginal-liver-grafts-under-stepwise-regulation-of-aldose-reductase-and-notch-signaling
#17
Xiao-Bing Liu, Chung-Mau Lo, Qiao Cheng, Kevin Tak-Pan Ng, Yan Shao, Chang-Xian Li, Sookja K Chung, Irene Oi Lin Ng, Jun Yu, Kwan Man
Background and Aims: Expanded donor criteria poses increased risk for late phase complications such as fibrosis that lead to graft dysfunction in liver transplantation. There remains a need to elucidate the precise mechanisms of post-transplant liver damage in order to improve the long-term outcomes of marginal liver grafts. In this study, we aimed to examine the role of oval cells in fibrogenic development of marginal liver grafts and explore the underlying mechanisms. Methods: Using an orthotopic rat liver transplantation model and human post-transplant liver biopsy tissues, the dynamics of oval cells in marginal liver grafts was evaluated by the platform integrating immuno-labeling techniques and ultrastructure examination...
2017: Theranostics
https://www.readbyqxmd.com/read/29179487/parp-1-promotes-tumor-recurrence-after-warm-ischemic-liver-graft-transplantation-via-neutrophil-recruitment-and-polarization
#18
Shuai Wang, Fa-Ji Yang, Xun Wang, Yuan Zhou, Bo Dai, Bing Han, Hu-Cheng Ma, Yi-Tao Ding, Xiao-Lei Shi
Poly (ADP-ribose) polymerase 1 (PARP-1) is a crucial contributor to exacerbate ischemia and reperfusion (IR) injury and cancer process. However, there is little research into whether PARP-1 affects the hepatocellular carcinoma (HCC) recurrence after liver transplantation. In this study, we investigated the influence of PARP-1 on hepatic neutrophil mobilizing and phenotype shifting which may lead to HCC recurrence after liver transplantation. We found that rats received the grafts with warm ischemic injury had higher risk of HCC recurrence, which was markedly prevented by pharmacological inhibition of PARP-1 after liver transplantation...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29174380/effect-of-heme-oxygenase-1-on-the-protection-of-ischemia-reperfusion-injury-of-bile-duct-in-rats-after-liver-transplantation
#19
Xi Zhan, Zhiqing Zhang, Hanfei Huang, Yujun Zhang, Zhong Zeng
OBJECTIVE: To investigate the effect of heme oxygenase-1 (HO-1) on the ischemic reperfusion injury (IRI) of bile duct in rat models after liver transplantation. METHODS: 320 SD rats were equally and randomly divided into 5 groups, which were group A receiving injection of 3×108/pfu/ml adenovirus (adv), group B with donor receiving Adv-HO-1 and recipient receiving Adv-HO-1-siRNA, group C with donor and recipient both receiving Adv-HO-1, group D with donor receiving Adv-HO-1-siRNA and recipient receiving Adv-HO-1, and group E with donor and recipient both receiving Adv-HO-1-siRNA at 24h before liver transplantation...
November 21, 2017: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/29168056/in-vivo-therapeutic-potential-of-inula-racemosa-in-hepatic-ischemia-reperfusion-injury-following-orthotopic-liver-transplantation-in-male-albino-rats
#20
Zhuoyi Wang, Lei Geng, Zhiyun Chen, Bingyi Lin, Mangli Zhang, Shusen Zheng
Hepatic ischemia-reperfusion (I/R) injury mainly occurs following hepatic resection and liver transplantation and cause severe liver damage, organ injuries, and dysfunction. Pro-inflammatory cytokines that promote injury are released when kupffer cell activates after getting induced by I/R. Repercussions of oxidative stress and cardiac function against isoproterenol based myocardial infarction are caused by flavonol glycosides which are found in high concentrations in Inula racemosa (Ir).The root was deemed to have analgesic and anti-inflammatory effects, and no report has been published about the liver-protective activity against hepatic I/R...
November 22, 2017: AMB Express
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