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https://www.readbyqxmd.com/read/28819440/microrna-204-5p-inhibits-invasion-and-metastasis-of-laryngeal-squamous-cell-carcinoma-by-suppressing-forkhead-box-c1
#1
Wei Gao, Yongyan Wu, Xiaoling He, Chunming Zhang, Meixia Zhu, Bo Chen, Qingqing Liu, Xukuan Qu, Weiyan Li, Shuxin Wen, Binquan Wang
Background and aim: Understanding the molecular biological mechanisms underlying laryngeal squamous cell carcinoma (LSCC) invasion and metastasis is crucial for diagnosis, treatment, and prognosis. We aimed to examine the expression of the tumor suppressor microRNA-204-5p (miR-204-5p) and its target gene, forkhead box C1 (FOXC1), in human LSCC and explore their roles in the malignant behaviors of LSCC Hep-2 and TU-177 cells. Methods: The regulatory effects of miR-204-5p on the 3' untranslated region of FOXC1 predicted by bioinformatics were tested by dual-luciferase reporter assay...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28818113/respiratory-syncytial-virus-rsv-entry-is-inhibited-by-serine-protease-inhibitor-aebsf-when-present-during-an-early-stage-of-infection
#2
Winke Van der Gucht, Annelies Leemans, Marjorie De Schryver, Annick Heykers, Guy Caljon, Louis Maes, Paul Cos, Peter L Delputte
BACKGROUND: Host proteases have been shown to play important roles in many viral activities such as entry, uncoating, viral protein production and disease induction. Therefore, these cellular proteases are putative targets for the development of antivirals that inhibit their activity. Host proteases have been described to play essential roles in Ebola, HCV, HIV and influenza, such that specific protease inhibitors are able to reduce infection. RSV utilizes a host protease in its replication cycle but its potential as antiviral target is unknown...
August 17, 2017: Virology Journal
https://www.readbyqxmd.com/read/28811224/hypericin-cytotoxicity-in-tumor-and-non-tumor-cell-lines-a-chemometric-study
#3
Joyce Laura da Silva Gonçalves, Claudia Bernal, Hidetake Imasatoa, Janice Rodrigues Perussi
Hypericin (HY) is an excellent photoactive compound that has been investigated for the photodynamic treatment of cancer as well as for microorganisms inactivation. In this study, chemometric analysis was applied for the first time on photodynamic assays to investigate the cytotoxicity of HY in tumor (HEp-2) and non-tumor (Vero and HUVEC) cell lines. The experimental planning was based on eight assays using the 2(3) full factorial design combining three important variables for PDT: photosensitizer concentrations, incubation time of cells in HY solutions and employed light dose (=590±10nm)...
August 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28806864/study-of-physical-function-in-adolescents-with-haemophilia-the-so-fit-study
#4
K Khair, M Holland, M Bladen, A Griffioen, P McLaughlin, S von Mackensen
INTRODUCTION: Contemporary haemophilia care demands Patient-Reported Outcomes. SO-FIT is a UK multi-centre study, assessing self-reported function, health-related quality of life (HRQoL) and joint health in boys with severe haemophilia. METHODS: Subjective physical function (PedHAL, HEP-Test-Q) and HRQoL (Haemo-QoL Short Form [SF]) were assessed alongside joint health using the objective Haemophilia Joint Health Score (HJHS v2.1). Demographic and clinical data were collected...
August 14, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28806534/knockdown-of-snail-inhibits-epithelial-mesenchymal-transition-of-human-laryngeal-squamous-cell-carcinoma-hep-2-cells-through-vdr-signaling-pathway
#5
Xue Zhao, Dan Yu, Jingpu Yang, Kai Xue, Yan Liu, Chunshun Jin
It has been well-documented that Snail plays a decisive role in various tumors. However, the direct effect of Snail on laryngeal squamous cell carcinoma (LSCC) has not been elaborated. In this study, we firstly detected the expression of Snail in 14 samples of patients with LSCC and found that its content was high in cancer tissues compared with adjacent tissues. Then we established LSCC Hep-2 cells with Snail silencing and validated the knockdown efficiency by western blotting and real-time PCR. Results showed that silencing of Snail significantly inhibited the ability of adhesion, migration, and invasion of Hep-2 cells...
August 14, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28805868/a-microfluidic-flow-cytometer-enabling-absolute-quantification-of-single-cell-intracellular-proteins
#6
Xiufeng Li, Beiyuan Fan, Shanshan Cao, Deyong Chen, Xiaoting Zhao, Dong Men, Wentao Yue, Junbo Wang, Jian Chen
Quantification of single-cell proteomics provides key insights into cellular heterogeneity while conventional flow cytometry cannot provide absolute quantification of intracellular proteins of single cells due to the lack of calibration approaches. This paper presents a constriction channel (with a cross sectional area smaller than cells) based microfluidic flow cytometer, capable of collecting copy numbers of specific intracellular proteins. In this platform, single cells stained with fluorescence labelled antibodies were forced to squeeze through the constriction channel with the fluorescence intensities quantified and since cells fully filled the constriction channel during the squeezing process, solutions with fluorescence labelled antibodies were flushed into the constriction channel to obtain calibration curves...
August 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28804570/value-of-quality-of-life-analysis-in-liver-cancer-a-clinician-s-perspective
#7
REVIEW
Leung Li, Winnie Yeo
Health related quality of life (HRQOL) is increasingly recognized as an important clinical parameter and research endpoint in patients with hepatocellular carcinoma (HCC). HRQOL in HCC patients is multifaceted and affected by medical factor which encompasses HCC and its complications, oncological and palliative treatment for HCC, underlying liver disease, as well as the psychological, social or spiritual reaction to the disease. Many patients presented late with advanced disease and limited survival, plagued with multiple symptoms, rendering QOL a very important aspect in their general well being...
July 18, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28802074/altered-amino-acid-concentrations-in-nafld-impact-of-obesity-and-insulin-resistance
#8
Melania Gaggini, Fabrizia Carli, Chiara Rosso, Emma Buzzigoli, Milena Marietti, Veronica Della Latta, Demetrio Ciociaro, Maria Lorena Abate, Roberto Gambino, Maurizio Cassader, Elisabetta Bugianesi, Amalia Gastaldelli
Plasma concentrations of amino acids (AA), in particular branched chain (BCAA), are often found increased in non-alcoholic fatty liver disease (NAFLD). However, if this is due to increased muscular protein catabolism, obesity and/or increased insulin resistance (IR) or impaired tissue metabolism is not known. Thus, we evaluated a) if subjects with NAFLD non-obese (NAFLD-NO), compared to obese (NAFLD-Ob) display altered plasma AA compared to controls (CT); b) if AA concentrations are associated to IR and liver histology...
August 12, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28800461/design-synthesis-and-biological-evaluation-of-novel-3-substituted-imidazo-1-2-a-pyridine-and-quinazolin-4-3h-one-derivatives-as-pi3k%C3%AE-inhibitors
#9
Yan-Hua Fan, Wei Li, Dan-Dan Liu, Meng-Xuan Bai, Hong-Rui Song, Yong-Nan Xu, SangKook Lee, Zhi-Peng Zhou, Jian Wang, Huai-Wei Ding
Phosphatidylinositol 3-kinase (PI3K) is a pivotal regulator of intracellular signaling pathways and considered as a promising target in the development of a therapeutic treatment of cancer. Among the different PI3K subtypes, the PIK3CA gene encoding PI3K p110α is frequently mutated and overexpressed in majority of human cancers. Therefore, the inhibition of PI3Kα has been considered to be an efficient approach for the treatment of cancer. In this study, two series compounds containing hydrophilic group in imidazo[1,2-a]pyridine and quinazolin-4(3H)-one were synthesized and their antiproliferative activities against five cancer cell lines, including HCT-116, SK-HEP-1, MDA-MB-231, SNU638 and A549, were evaluated...
August 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28795320/ezh2-promotes-cell-proliferation-by-regulating-the-expression-of-runx3-in-laryngeal-carcinoma
#10
Rong Lian, Huimin Ma, Zhiyan Wu, Guozheng Zhang, Lei Jiao, Wenjie Miao, Qianqian Jin, Ruixue Li, Ping Chen, Haixu Shi, Wenfa Yu
Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 and the tumor suppressor RUNX3...
August 9, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28789962/expression-and-characterization-of-an-enhanced-recombinant-heparinase-i-with-chitin-binding-domain
#11
Shuqin Xu, Meiling Qiu, Xuanyue Zhang, Jinghua Chen
Heparinase I (Hep I) can efficiently depolymerize heparin and heparin sulfate to oligosaccharides or unsaturated disaccharides, which resulted in loss of physiological function such as blood coagulation. In order to realize the immobilization of Hep I on chitin carriers, we cloned Hep I with the chitin binding domain (ChBD) as a chitin-affinity tag, and the Small Ubiquitin-like MOdifier (SUMO) linker as a solvation enhancer in different fusion sequence. DNA and protein gels suggested that 4 kinds of recombinants were successfully constructed and expressed in Escherichia coli (E...
August 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28780484/expression-of-hepcidin-and-ferroportin-in-full-term-placenta-of-pregnant-cows
#12
Sante Roperto, Valeria Russo, Chiara Urraro, Anna Cutarelli, Antonella Perillo, Francesca De Falco, Franco Roperto
Hepcidin (HEP) and ferroportin (FPN) play a central role in systemic iron homeostasis. The HEP/FPN axis controls both extracellular iron concentration and total body iron levels. HEP is synthesized mainly by hepatocytes and controls the absorption of dietary iron and the distribution of iron to the various cell types; its synthesis is regulated by both iron and innate immunity. FPN is a membrane protein and the major exporter of iron from mammalian cells, including iron recycling macrophages, iron absorbing duodenal enterocytes, and iron storing hepatocytes...
July 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28778116/caveolin-1-restricts-group-a-streptococcus-invasion-of-non-phagocytic-host-cells
#13
Jin Yan Lim, Timothy C Barnett, Michele Bastiani, Kerrie-Ann McMahon, Charles Ferguson, Richard I Webb, Robert G Parton, Mark J Walker
Caveolae are composed of two major proteins, caveolin-1 (CAV1) and cavin-1/polymerase transcript factor I (CAVIN1). Here we demonstrate that CAV1 levels modulate invasion of group A Streptococcus (GAS) into non-phagocytic mammalian cells. GAS showed enhanced internalization into CAV1 knockout mouse embryonic fibroblasts (MEFs), and CAV1 knockdown human epithelial HEp-2 cells, whereas over-expression of CAV1 in HEp-2 cells reduced GAS invasion. This effect was not dependent on the expression of the GAS fibronectin binding protein SfbI, which had previously been implicated in caveolae-mediated uptake...
August 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28776672/mechanisms-involved-in-the-cytotoxic-action-of-brazilian-propolis-and-caffeic-acid-against-hep-2-cells-and-modulation-of-p-glycoprotein-activity
#14
Lívia M da Silva, Yahima Frión-Herrera, Ariane R Bartolomeu, Carolina Mendonça Gorgulho, José M Sforcin
OBJECTIVES: The effects of propolis and phenolic compounds (caffeic acid - Caf; dihydrocinnamic acid - Cin; p-coumaric acid - Cou) in the same quantity found in our propolis sample were investigated on human laryngeal epidermoid carcinoma (HEp-2) cells. METHODS: Cell viability, apoptosis/necrosis and cell cycle arrest, P53 and CASPASE-3 gene expression, generation of reactive oxygen species (ROS) and the ability of propolis to induce doxorubicin (DOX) efflux using a P-glycoprotein (P-gp) inhibitor (verapamil) were assayed...
August 4, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28776296/synthesis-of-piperazine-functionalized-magnetic-sporopollenin-a-new-organic-inorganic-hybrid-material-for-the-removal-of-lead-ii-and-arsenic-iii-from-aqueous-solution
#15
Naqhiyah Farhan Ahmad, Muhammad Afzal Kamboh, Hamid Rashidi Nodeh, Siti Nadiah Binti Abd Halim, Sharifah Mohamad
The present work describes the successful functionalization/magnetization of bio-polymeric spores of Lycopodium clavatum (sporopollenin) with 1-(2-hydroxyethyl) piperazine. Analytical techniques, i.e., Fourier transform infrared (FT-IR), field emission scanning electron microscope (FESEM), energy-dispersive X-ray spectroscopy (EDS), and vibrating sample magnetometer (VSM), were used to confirm the formation of 1-(2-hydroxyethyl) piperazine-functionalized magnetic sporopollenin (MNPs-Sp-HEP). The proposed adsorbent (MNPs-Sp-HEP) was used for the removal of noxious Pb(II) and As(III) metal ions from aqueous media through a batch-wise method...
August 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28774308/polarity-directed-optimization-of-phytochemical-and-in-vitro-biological-potential-of-an-indigenous-folklore-quercus-dilatata-lindl-ex-royle
#16
Madiha Ahmed, Humaira Fatima, Muhammad Qasim, Bilquees Gul, Ihsan-Ul-Haq
BACKGROUND: Plants have served either as a natural templates for the development of new chemicals or a phytomedicine since antiquity. Therefore, the present study was aimed to appraise the polarity directed antioxidant, cytotoxic, protein kinase inhibitory, antileishmanial and glucose modulatory attributes of a Himalayan medicinal plant- Quercus dilatata. METHODS: Total phenolic and flavonoid contents were determined colorimetrically and various polyphenols were identified by RP-HPLC analysis...
August 3, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28771196/preparation-and-evaluation-of-dexamethasone-dex-growth-and-differentiation-factor-5-gdf-5-surface-modified-titanium-using-%C3%AE-cyclodextrin-conjugated-heparin-cd-hep-for-enhanced-osteogenic-activity-in-vitro-and-in-vivo
#17
Dae Hyeok Yang, Sun-Jung Yoon, Deok-Won Lee
The most ideal implant models in the dental and orthopedic fields to minimize the failure rate of implantation involve the improvement of osseointegration with host bone. Therefore, a focus of this study is the preparation of surface-modified titanium (Ti) samples of disc and screw types using dexamethasone (DEX) and/or growth and differentiation factor-5 (GDF-5), as well as the evaluation of their efficacies on bone formation in vitro and in vivo. X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and contact angle measurement were used to evaluate the surface chemical composition, surface morphology and wettability, respectively...
August 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28770960/expression-of-mir-195-in-laryngeal-squamous-cell-carcinoma-and-its-effect-on-proliferation-and-apoptosis-of-hep-2
#18
Y Shuang, C Li, X Zhou, Y-W Huang, L Zhang
OBJECTIVE: To investigate the expression of miR-195 and its relationship with clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC), and to explore its effect and possible mechanism on proliferation and apoptosis of Hep-2. PATIENTS AND METHODS: Real-time fluorescence quantitative PCR was used to detect the expression of miR-195 in laryngeal carcinoma tissues and adjacent normal tissues from 98 cases. Dual-luciferase reporter plasmid with Bcl-2 wild type and mutant type 3' untranslated region was created to verify the target of miR-195 by luciferase assay...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28770702/prevalence-of-anti-dfs70-antibodies-in-patients-with-and-without-systemic-autoimmune-rheumatic-diseases
#19
Ora Shovman, Boris Gilburd, Chen Chayat, Howard Amital, Pnina Langevitz, Abdulla Watad, Adi Guy, Dolores Perez, Danielle Azoulay, Miri Blank, Yael Segal, Chelsea Bentow, Michael Mahler, Yehuda Shoenfeld
OBJECTIVES: Autoantibodies to the dense fine speckled 70 (DFS70) antigen are common among antinuclear antibodies (ANA) positive healthy individuals (HI). We assessed the prevalence of anti-DFS70 antibodies in patients with and without ANA-associated rheumatic diseases (AARDs) by two methods: chemiluminescent immunoassay (CIA) and an indirect immunofluorescence (IIF) assay based on immunoadsorption for DFS70. METHODS: Fifty-one ANA-positive sera samples from patients with confirmed clinical diagnosis of AARD, 92 samples from HI and 85 samples submitted to a reference laboratory for routine ANA testing were evaluated for the presence of anti-DFS70 antibodies...
July 27, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28769749/attention-in-and-out-scalp-level-and-intracranial-eeg-correlates-of-interoception-and-exteroception
#20
Indira García-Cordero, Sol Esteves, Ezequiel P Mikulan, Eugenia Hesse, Fabricio H Baglivo, Walter Silva, María Del Carmen García, Esteban Vaucheret, Carlos Ciraolo, Hernando S García, Federico Adolfi, Marcos Pietto, Eduar Herrera, Agustina Legaz, Facundo Manes, Adolfo M García, Mariano Sigman, Tristán A Bekinschtein, Agustín Ibáñez, Lucas Sedeño
Interoception, the monitoring of visceral signals, is often presumed to engage attentional mechanisms specifically devoted to inner bodily sensing. In fact, most standardized interoceptive tasks require directing attention to internal signals. However, most studies in the field have failed to compare attentional modulations between internally- and externally-driven processes, thus probing blind to the specificity of the former. Here we address this issue through a multidimensional approach combining behavioral measures, analyses of event-related potentials and functional connectivity via high-density electroencephalography, and intracranial recordings...
2017: Frontiers in Neuroscience
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