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https://www.readbyqxmd.com/read/28108407/purification-and-characterization-of-an-extracellular-ribonuclease-from-a-bacillus-sp-rns3-kx966412
#1
Gupta Shruti, Singh Sukhdev, Kanwar Shamsher Singh
Ribonucleases (RNases) catalyze the degradation of ribonucleic acid (RNA) into smaller nucleotides. RNases display angiogenic, neurotoxic, antitumor and immunosuppressive properties. In the present study, an extracellular RNase was successfully purified to homogeneity from a Bacillus sp. RNS3 (KX966412) by salting out at 0-50% ammonium sulphate saturation followed by the gel permeation (Sephadex G-100) chromatography. The multistep purification resulted in 10.4 fold purification of RNase with a yield of 3.12%...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28106938/vascular-endothelial-growth-factor-targeted-paclitaxel-loaded-liposome-microbubbles-and-inhibition-of-human-epidermoid-2-cell-proliferation
#2
Lian Zhou, Wenlong Luo
BACKGROUND: Vascular endothelial growth factor (VEGF) plays an important role in the formation of capillary blood vessels. The purpose of this study was to evaluate the inhibitory effect of VEGF-targeted paclitaxel (PTX)-loaded liposome microbubbles (VTPLLMs) on the proliferation of human epidermoid (Hep-2) laryngeal squamous cell carcinoma (SCC). METHODS: Six groups were randomly divided. The inhibitory effects on Hep-2 proliferation were assessed by a methylthiazol tetrazolium (MTT) method...
January 20, 2017: Head & Neck
https://www.readbyqxmd.com/read/28103717/baccharis-trimera-inhibits-reactive-oxygen-species-production-through-pkc-and-down-regulation-p47-phox-phosphorylation-of-nadph-oxidase-in-sk-hep-1-cells
#3
Glaucy Rodrigues de Araújo, Ana Carolina Silveira Rabelo, Janaína Serenato Meira, Joamyr Victor Rossoni-Júnior, William de Castro-Borges, Renata Guerra-Sá, Maurício Azevedo Batista, Denise da Silveira-Lemos, Gustavo Henrique Bianco de Souza, Geraldo Célio Brandão, Míriam Martins Chaves, Daniela Caldeira Costa
Baccharis trimera, popularly known as "carqueja", is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells...
February 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28102028/the-involvement-of-msmk-in-pathogenesis-of-the-streptococcus-suis-serotype-2
#4
Mei-Fang Tan, Wan-Quan Liu, Chun-Yan Zhang, Ting Gao, Lin-Lin Zheng, De-Xin Qiu, Lu Li, Rui Zhou
Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen that causes global economic and public health problems. Virulent S. suis strains successfully maintain high bacterial concentrations in host blood and rapidly adapt to challenging environments within hosts. Successful survival in hosts is a major factor influencing the pathogenesis of SS2. We have previously identified that SS2 colonization in mouse brain is possibly affected by the ATPase, MsmK of carbohydrate ATP-binding cassette (ABC) transporters because of carbohydrate utilization...
January 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28101799/antitumor-efficacy-of-slpi-promoter-controlled-expression-of-artificial-microrna-targeting-egfr-in-a-squamous-cell-carcinoma-cell-line
#5
Jia Chen, Shoude Zhang, Yi Lin, Beibei Yang, Jiang Cao
The purpose of this study was to develop a recombinant adenovirus with secretory leukoprotease inhibitor (SLPI) promoter-controlled expression for gene therapy of squamous cell carcinoma (SCC). An artificial microRNA targeting epidermal growth factor receptor (EGFR) was designed, and used to construct a replication-defective recombinant adenovirus with SLPI promoter-controlled expression. The silencing efficiency of this vector (Ad-SLPI-EGFRamiR) was detected in Hep-2 cells. Western blotting showed that the expression of 170 kD EGFR was significantly reduced in Hep-2 cells 72 h after infection with Ad-SLPI-EGFRamiR...
January 18, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28093234/the-1910hk-rr-two-component-system-is-essential-for-the-virulence-of-streptococcus-suis-serotype-2
#6
Fangyan Yuan, Chen Tan, Zewen Liu, Keli Yang, Danna Zhou, Wei Liu, Zhengying Duan, Rui Guo, Huanchun Chen, Yongxiang Tian, Weicheng Bei
Streptococcus suis serotype 2 is a major zoonotic pathogen, and the two-component system plays an important role in bacterial pathogenesis. The present study targeted the 1910HK/RR two-component system of S. suis 2. A 1910HK/RR deletion mutant (Δ1910HK/RR) and the corresponding complementation strain (CΔ1910HK/RR) were constructed in S. suis 2 strain 05ZYH33. 1910HK/RR deletion had no effect on S. suis 2 growth, but significantly inhibited the adherence and invasion of S. suis 2 to HEp-2 cells. Analysis of the role of 1910HK/RR in murine and pig infection models demonstrated that 1910HK/RR played a distinct role in the virulence of S...
January 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28089932/selenizing-hericium-erinaceus-polysaccharides-induces-dendritic-cells-maturation-through-mapk-and-nf-%C3%AE%C2%BAb-signaling-pathways
#7
Tao Qin, Zhe Ren, Yifan Huang, Yulong Song, Dandan Lin, Jian Li, Yufang Ma, Xiuqin Wu, Fuan Qiu, Qi Xiao
In this study, polysaccharides extracted from Hericium erinaceus were modified to obtain its nine selenium derivatives, sHEP1-sHEP9. Their structures were identified, yields and selenium contents were determined, the phenotypic and functional maturation of murine bone marrow-derived dendritic cells (DCs) and relevant mechanisms were compared taking unmodified HEP as control. The results revealed that the selenylation were successful. sHEP1, sHEP2 and sHEP8 treatment of DCs increased their surface expression of MHC-II and CD86 and indicated that sHEP1, sHEP2 and sHEP8 induced DC maturation...
January 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28089546/expression-of-interleukin-6-by-a-recombinant-rabies-virus-enhances-its-immunogenicity-as-a-potential-vaccine
#8
Jun Luo, Boyue Zhang, Yuting Wu, Qin Tian, Jing Zhao, Ziyu Lyu, Qiong Zhang, Mingzhu Mei, Yongwen Luo, Xiaofeng Guo
Several studies have confirmed that interleukin-6 (IL6) mediates multiple biological effects that enhance immune responses when used as an adjuvant. In the present study, recombinant rabies virus (RABV) expressing canine IL6 (rHEP-CaIL6) was rescued and its pathogenicity and immunogenicity were investigated in mice. We demonstrated that mice received a single intramuscular immunization with rHEP-CaIL6 showed an earlier increase and higher maximum titres of virus-neutralizing antibody (VNA) as well as anti-RABV antibodies compared with mice immunized with the parent strain...
January 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28087493/antioxidant-and-antiproliferative-potential-of-biosurfactants-isolated-from-lactobacillus-casei-and-their-anti-biofilm-effect-in-oral-staphylococcus-aureus-strains
#9
Abderrahmen Merghni, Ines Dallel, Emira Noumi, Yassine Kadmi, Hajer Hentati, Samir Tobji, Adel Ben Amor, Maha Mastouri
Biosurfactants also called bioemulsifiers are amphipathic compounds produced by many microorganisms that allow them to exhibit a wide range of biological activities. The aim of this study was to determine the antioxidant and antiproliferative potential of biosurfactants isolated from Lactobacillus casei and to assess their anti-adhesive and anti-biofilm abilities against oral opportunistic Staphylococcus aureus strains. The antioxidant activity of biosurfactant was evaluated using the in vitro scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical...
January 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28081204/deletion-of-ssna-attenuates-the-pathogenicity-of-streptococcus-suis-and-confers-protection-against-serovar-2-strain-challenge
#10
Miao Li, Ru-Jian Cai, Chun-Ling Li, Shuai Song, Yan Li, Zhi-Yong Jiang, Dong-Xia Yang
Streptococcus suis serotype 2 (SS2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. Streptococcus suis nuclease A (SsnA) is a recently discovered deoxyribonuclease (DNase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (NETs). To further determine the effects of ssnA on virulence, the ssnA deletion mutant (ΔssnA) and its complemented strain (C-ΔssnA) were constructed. The ability of ΔssnA mutant to interact with human laryngeal epithelial cell (Hep-2) was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28080973/changes-in-interoceptive-processes-following-brain-stimulation
#11
Olga Pollatos, Beate M Herbert, Sandra Mai, Thomas Kammer
The processing and perception of individual internal bodily signals (interoception) has been differentiated to comprise different levels and processes involved. The so-called heartbeat-evoked potential (HEP) offers an additional possibility to examine automatic processing of cardiac signals. Knowledge on neural structures potentially supporting different facets of interoception is still sparse. One way to get insights into neuroanatomical function is to manipulate the activity of different brain structures...
November 19, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28080965/feeling-learning-from-and-being-aware-of-inner-states-interoceptive-dimensions-in-neurodegeneration-and-stroke
#12
Indira García-Cordero, Lucas Sedeño, Laura de la Fuente, Andrea Slachevsky, Gonzalo Forno, Francisco Klein, Patricia Lillo, Jesica Ferrari, Clara Rodriguez, Julian Bustin, Teresa Torralva, Sandra Baez, Adrian Yoris, Sol Esteves, Margherita Melloni, Paula Salamone, David Huepe, Facundo Manes, Adolfo M García, Agustín Ibañez
Interoception is a complex process encompassing multiple dimensions, such as accuracy, learning and awareness. Here, we examined whether each of those dimensions relies on specialized neural regions distributed throughout the vast interoceptive network. To this end, we obtained relevant measures of cardiac interoception in healthy subjects and patients offering contrastive lesion models of neurodegeneration and focal brain damage: behavioural variant fronto-temporal dementia (bvFTD), Alzheimer's disease (AD) and fronto-insular stroke...
November 19, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28073324/3-methoxy-aroylhydrazones-free-radicals-scavenging-anticancer-and-cytoprotective-potency
#13
Nadya Hristova-Avakumova, Krassimira Yoncheva, Boryana Nikolova-Mladenova, Trayko Traykov, Georgi Momekov, Vera Hadjimitova
OBJECTIVE: This study aimed to determine the capability of newly designed 3-methoxy derivatives of salicylaldehyde benzoylhydrazone to influence the oxidative stress processes and to test their in vitro cytotoxicity. METHODS: We have used chemiluminescent and spectrophotometric model systems containing different types of reactive oxygen species (OH(●), OCl(─) and O2(─●)). The hydrazones effect on the viability of Hep-G2, HEK-293 and SH-SY5Y cell lines was determined via MTT assay...
January 10, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28072492/identification-and-characterization-of-novel-host-defense-peptides-from-the-skin-secretion-of-the-fungoid-frog-hydrophylax-bahuvistara-anura-ranidae
#14
T V Vineeth Kumar, R Asha, G Shyla, Sanil George
Two novel peptides (brevinin1 HYba1 and brevinin1 HYba2) were identified from the skin secretion of the frog Hydrophylax bahuvistara, endemic to Western Ghats, India, and their amino acid sequences were confirmed using cDNA cloning and LC/MS/MS. Antibacterial, hemolytic and cytotoxic activities of brevinin1 peptides and their synthetic analogs (amidated C-terminus) were investigated and compared. All the peptides except the acidic forms showed antibacterial activity against all tested Gram -positive and negative bacteria...
January 10, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28066964/mechanisms-of-the-prevention-and-inhibition-of-the-progression-and-development-of-nash-by-genetic-and-pharmacological-dcr3-supplementation
#15
Pei-Chang Lee, Ling-Yu Yang, Ying-Wen Wang, Shiang-Fen Huang, Kuei-Chuan Lee, Yun-Cheng Hsieh, Ying-Ying Yang, Shie-Liang Hsieh, Ming-Chih Hou, Han-Chieh Lin, Fa-Yuah Lee, Shou-Dong Lee
AIMS: Treatment to non-alcoholic steatohepatitis (NASH) is difficult due to the absence of a proven treatment and its comprehensive mechanisms. In the NASH animal model, up-regulated hepatic inflammation and oxidative stress with the resultant M1 polarization of macrophage as well as the imbalanced adipocytokines all accelerate NASH progression. As a member of TNF receptor superfamily, decoy receptor 3 (DcR3) not only neutralizes the death ligands, but also performs immune modulations...
January 8, 2017: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/28062866/-bovine-lactoferrin-decreases-the-invasion-of-salmonella-enterica-to-hep-2-cells
#16
Liz J Barreto Arce, Carmen A Contreras García, David Durand Vara, Theresa Ochoa Woodell
OBJECTIVE: To assess the effect of bovine lactoferrin (bLf) on the invasion of Salmonella enterica ser. Typhimurium to HEp-2 cells. MATERIALS AND METHODS: HEp-2 monolayers were infected with 106 colony forming unit (CFU) of bacteria in the absence and presence of 1 and 10 mg/mL of bLf (iron-saturated) and incubated 1.5 hours at 37°C. Two treatments were evaluated: pre- infection (HEp-2 cells were incubated with bLf one hour prior to infection with Salmonella) and post-infection (bLf was added 15 minutes after the infection)...
October 2016: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/28062290/virulence-related-genes-adhesion-and-invasion-of-some-yersinia-enterocolitica-like-strains-suggests-its-pathogenic-potential
#17
Priscilla F M Imori, Jaqueline Passaglia, Roberto A Souza, Lenaldo B Rocha, Juliana P Falcão
Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cells. A total of 50 Y. frederiksenii, 55 Y. intermedia and 13 Y. kristensenii strains were studied. The strains contained the following genes: Y. frederiksenii, fepA(44%), fes(44%) and ystB(18%); Y...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28059504/particulate-photocatalyst-sheets-based-on-carbon-conductor-layer-for-efficient-z-scheme-pure-water-splitting-at-ambient-pressure
#18
Qian Wang, Takashi Hisatomi, Yohichi Suzuki, Zhenhua Pan, Jeongsuk Seo, Masao Katayama, Tsutomu Minegishi, Hiroshi Nishiyama, Tsuyoshi Takata, Kazuhiko Seki, Akihiko Kudo, Taro Yamada, Kazunari Domen
Development of sunlight-driven water splitting systems with high efficiency, scalability, and cost-competitiveness is a central issue for mass production of solar hydrogen as a renewable and storable energy carrier. Photocatalyst sheets comprising a particulate hydrogen evolution photocatalyst (HEP) and an oxygen evolution photocatalyst (OEP) embedded in a conductive thin film can realize efficient and scalable solar hydrogen production using Z-scheme water splitting. However, the use of expensive precious metal thin films that also promote reverse reactions is a major obstacle to developing a cost-effective process at ambient pressure...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28057904/a-serine-threonine-phosphatase-1-of-streptococcus-suis-type-2-is-an-important-virulence-factor
#19
Lihua Fang, Pengcheng Fan, Yunkai Yang, Jingjing Zhou, Hongxia Shen, Weihuan Fang
Streptococcus suis is regarded as one of the major pathogens of pigs while S. suis type 2 (SS2) is considered to be a zoonotic bacterium for its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. Many bacterial species contain genes encoding serine/threonine protein phosphatases (STP) responsible for dephosphorylation of their substrates in a single reaction step. This study was aimed to investigate the role of stp1 in pathogenesis of SS2. An isogenic stp1 mutant (Δstp1) was constructed from the SS2 strain ZJ081101...
January 4, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28054985/novel-pei-poly-%C3%AE-gutamic-acid-nanoparticles-for-high-efficient-sirna-and-plasmid-dna-co-delivery
#20
Shu-Fen Peng, Hung-Kun Hsu, Chun-Cheng Lin, Ya-Ming Cheng, Kuang-Hsing Hsu
The efficient delivery of sufficient amounts of nucleic acids into target cells is critical for successful gene therapy and gene knockdown. The DNA/siRNA co-delivery system has been considered a promising approach for cancer therapy to simultaneously express and inhibit tumor suppressor genes and overexpressed oncogenes, respectively, triggering synergistic anti-cancer effects. Polyethylenimine (PEI) has been identified as an efficient non-viral vector for transgene expression. In this study, we created a very high efficient DNA/siRNA co-delivery system by incorporating a negatively-charged poly-γ-glutamic acid (γ-PGA) into PEI/nucleic acid complexes...
January 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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