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Hep A

Vu Minh Thanh, Thi Hiep Nguyen, Tuong Vi Tran, Uyen-Thi Phan Ngoc, Minh Nhat Ho, Thi Thinh Nguyen, Yen Nguyen Tram Chau, Van Thu Le, Ngoc Quyen Tran, Cuu Khoa Nguyen, Dai Hai Nguyen
In this report, poly(amide amine) (PAMAM) dendrimer and Heparin-grafted-monomethoxy polyethylene glycol (HEP-mPEG) were synthesized and characterized. In aqueous solution, the generation 4 PAMAM dendrimers (G4.0-PAMAM) existed as nanoparticles with particle size of 5.63nm. However, after electrostatic complexation with HEP-mPEG to form a core@shell structure G4.0-PAMAM@HEP-mPEG, the size of nanoparticles was significantly increased (73.82nm). The G4.0-PAMAM@HEP-mPEG nanoparticles showed their ability to effectively encapsulate doxorubicin (DOX) for prolonged and controlled release...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Huimin Ma, Rong Lian, Zhiyan Wu, Xiao Li, Wenfa Yu, Yun Shang, Xixia Guo
Enhancing the sensitivity of laryngeal cells to radiation is crucial for improving the efficacy of laryngeal carcinoma. MicroRNAs are known to play a major role in regulating cellular radiosensitivity. This study was designed to explore the effect and the molecular basis of miR-503 in the radiosensitivity of laryngeal carcinoma cells. Quantitative real-time polymerase chain reaction analysis showed that miR-503 expression was decreased in human laryngeal carcinoma cell lines Hep-2 and TU212, and the downregulation of miR-503 was also observed after irradiation...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Hanjun Cheng, Yufeng Liu, Yihui Hu, Yubin Ding, Shichao Lin, Wen Cao, Qian Wang, Jiangjiexing Wu, Faheem Muhammad, Xiaozhi Zhao, Dan Zhao, Zhe Li, Hang Xing, Hui Wei
Metal-organic framework (MOF) nanosheets are a class of two-dimensional (2D) porous and crystalline materials that hold promise for catalysis and biodetection. Although 2D MOF nanosheets have been utilized for in vitro assays, ways of engineering them into diagnostic tools for live animals are much less explored. In this work, a series of MOF nanosheets are successfully engineered into a highly sensitive and selective diagnostic platform for in vivo monitoring of heparin (Hep) activity. The iron-porphyrin derivative is selected as ligand to synthesize a series of archetypical MOF nanosheets with intrinsic heme-like catalytic sites, mimicking pe-roxidase...
October 10, 2017: Analytical Chemistry
Young-In Kim, Rajat Pareek, Ryan Murphy, Lisa Harrison, Eric Farrell, Robert Cook, John DeVincenzo
BACKGROUND: RSV viral load and disease severity associate, and the timing of viral load and disease run in parallel. An antiviral must be broadly effective against the natural spectrum of RSV genotypes and must attain concentrations capable of inhibiting viral replication within the human respiratory tract. OBJECTIVES: We evaluated a novel RSV fusion inhibitor, MDT-637, and compared it with ribavirin for therapeutic effect in vitro to identify relative therapeutic doses achievable in humans...
October 9, 2017: Influenza and Other Respiratory Viruses
M Infantino, M Manfredi, V Grossi, M Benucci, G Morozzi, E Tonutti, M Tampoia, N Bizzaro
BACKGROUND: Patients with suspected idiopathic inflammatory myopathies (IIM) are commonly tested for the presence of anti-nuclear antibodies (ANA) by indirect immunofluorescence (IIF) on HEp-2 cell substrates. However, ANA-IIF false negative tests may occur in IIM because some antigens, such as Jo1 and Ro52, may be scarcely expressed on HEp-2 cells. In addition, cytoplasmic staining is often not appropriately investigated by a specific antibody assay, leading to decreased clinical sensitivity of the ANA test...
October 3, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
Maedeh Khayyat Kholghi, Gillian Bartlett, Morgan Phillips, Jon Salsberg, Alex M McComber, Ann C Macaulay
Background: Kahnawà:ke is a Kanien'kehá:ka (Mohawk) community in Quebec, Canada. In 1997, the community-controlled Kateri Memorial Hospital Centre in partnership with the Kahnawake Education Center, and the Kahnawake Schools Diabetes Prevention Project (KSDPP) developed an elementary school diabetes prevention health education program, aimed to increase knowledge of Type 2 diabetes, healthy eating and active lifestyles. Long-term goals for KSDPP community and school interventions are to decrease obesity and diabetes...
July 24, 2017: Family Practice
B Salehi, S A Ayatollahi, A Segura-Carretero, F Kobarfard, M D M Contreras, M Faizi, M Sharifi-Rad, S A Tabatabai, J Sharifi-Rad
The genus Eremurus is native to Eastern Europe and temperate Asia. Particularly, Eremurus persicus (Joub. & Spach) Boiss. is highly valued in traditional foods and medicine. Scientific knowledge about E. persicus chemical composition and bioactivity is required. Therefore, the present study is aimed to determine the volatile composition of E. persicus essential oil (EO) by means of gas chromatography coupled to flame ionization/mass spectrometry detection. Moreover, the antioxidant, antimicrobial, anticancer, and acetylcholinesterase inhibitory activities of the EO were tested...
September 30, 2017: Cellular and Molecular Biology
Wei Jing, Jonathan W Roberts, Dixy E Green, Andrew Almond, Paul L DeAngelis
Many injectable drugs require delivery strategies for enhancing their pharmacokinetics due to rapid loss via renal filtration if possess low molecular weight (<60-70 kDa) and/or clearance by the body's components (e.g., proteases, antibodies, high-efficiency receptors) in their native form. FDA-approved polyethylene glycol [PEG] is a vehicle for improving therapeutics, but artificial polymers have potential biocompatibility and immunogenicity liabilities. Here we utilized a natural vertebrate carbohydrate, heparosan [HEP], the biosynthetic precursor of heparan sulfate and heparin, to enhance performance of a biologic drug...
August 23, 2017: Glycobiology
Fei Cao, Chang-Lun Shao, Yun-Feng Liu, Hua-Jie Zhu, Chang-Yun Wang
Vibrational circular dichroism (VCD) method has become robust and reliable alternative for the stereochemical characterization of natural products. In this paper, three new serrulatane-type diterpenoids, euplexaurenes A-C (1-3), and a known metabolite, anthogorgiene P (4), were obtained from the South China Sea gorgonian Euplexaura sp. GXWZ-05. The absolute configuration of C-11 in 1-4, which was difficult to be determined by common means due to the high conformational flexibility of the eight-carbon aliphatic chain attached at C-4, was determined by VCD method, suggesting a new horizon to define the absolute configurations of natural products possessing chains...
October 2, 2017: Scientific Reports
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani, Homa Frohesh Tehrani
BACKGROUND: Klebsiella oxytoca is an opportunistic pathogen which damages intestinal epithelium through producing cytotoxin tilivalline. This toxin plays a role in the pathogenesis of bacteria and is the main virulence factor which leads to antibiotic-associated hemorrhagic colitis progress. MATERIALS AND METHODS: In this study, we collected a total of 75 K. oxytoca strains isolated from the stool, urine, blood, wounds, and sputum and evaluated them in terms of the production of toxins; we detected their cytotoxic effects on HEp-2 cells...
September 29, 2017: Microbial Pathogenesis
Sri Devi Subramaniyan, Ashok Kumar Natarajan
INTRODUCTION: Diabetes mellitus, a major metabolic disorder associated with hyperglycaemia is one of the leading cause of death in many developed countries. However, use of natural phytochemicals have been proved to have a protective effect against oxidative damage. AIM: To investigate the effect of citral, a monoterpene on high glucose induced cytotoxicity and oxidative stress in human hepatocellular liver carcinoma (Hep G2) cell line. MATERIALS AND METHODS: Cells were treated with 50 mM concentration of glucose for 24 hours incubation following citral (30 μM) was added to confluent HepG2 cells...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
Federica Rinaldi, Patrizia Nadia Hanieh, Catia Longhi, Simone Carradori, Daniela Secci, Gokhan Zengin, Maria Grazia Ammendolia, Elena Mattia, Elena Del Favero, Carlotta Marianecci, Maria Carafa
The aim of the present work is to develop nanoemulsions (NEs), nanosized emulsions, manufactured for improving the delivery of active pharmaceutical ingredients. In particular, nanoemulsions composed of Neem seed oil, contain rich bioactive components, and Tween 20 as nonionic surfactant were prepared. A mean droplet size ranging from 10 to 100 nm was obtained by modulating the oil/surfactant ratio. Physicochemical characterisation was carried out evaluating size, ζ-potential, microviscosity, polarity and turbidity of the external shell and morphology, along with stability in simulated cerebrospinal fluid (CSF), activity of Neem oil alone and in NEs, HEp-2 cell interaction and cytotoxicity studies...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
Johnny Birch, Hörður Kári Harðarson, Sanaullah Khan, Marie-Rose Van Calsteren, Richard Ipsen, Christel Garrigues, Kristoffer Almdal, Maher Abou Hachem, Birte Svensson
Interactions of exopolysaccharides and proteins are of great importance in food science, but complicated to analyze and quantify at the molecular level. A surface plasmon resonance procedure was established to characterize binding of seven structure-determined, branched hetero-exopolysaccharides (HePSs) of 0.14-4.9MDa from lactic acid bacteria to different milk proteins (β-casein, κ-casein, native and heat-treated β-lactoglobulin) at pH 4.0-5.0. Maximum binding capacity (RUmax) and apparent affinity (KA,app) were HePS- and protein-dependent and varied for example 10- and 600-fold, respectively, in the complexation with native β-lactoglobulin at pH 4...
December 1, 2017: Carbohydrate Polymers
Shuqin Xu, Xuanyue Zhang, Bo Duan, Jinghua Chen
Using chitin-affinity interaction between triple-functional heparinase I (Hep I) and chitin, an engineered platform was prepared to produce controllable low molecular weight heparin (LMWH). Chitin microspheres with well-defined nanofibrils were fabricated through a "bottom up" pathway. An enhanced soluble protein, ChBD-SUMO-Hep I (CSH-I), was expressed in 3L batch fermentation with a high bioactivity of 2.5×10(3) IU/L. Chitin binding domain (ChBD) can specifically bind to chitin in noncovalent way, which leads to the immobilization and purification of enzyme in a single step...
December 1, 2017: Carbohydrate Polymers
Fengzhi Si, Jie Sun, Chunli Wang
MicroRNA (miR)-138 generally has a suppressive role in various human cancer types; however, its role and the underlying mechanisms in laryngeal squamous cell carcinoma (LSCC) have remained to be elucidated. The present study assessed the clinical significance and regulatory mechanisms of miR-138 in LSCC progression. Reverse-transcription quantitative polymerase chain reaction analysis indicated that miR-138 was significantly downregulated in LSCC tissues and cell lines. In addition, the decreased expression of miR-138 was significantly associated with poor differentiation, lymph node metastasis and advanced clinical stage of LSCC...
September 2017: Experimental and Therapeutic Medicine
Shu-Wen Xie, Yan-Ni Wang, Hui-Yan Luo, Zi-Bin Lu, Lin-Zhong Yu, Jun-Shan Liu
OBJECTIVE: To investigate the inhibitory effect of giganteaside D (GD) on hepatocellular carcinoma and its molecular mechanisms. METHODS: The inhibitory effects of GD on Hep 3b cells were determined using MTT assay and colony formation assay. The morphological changes of Hep 3b cells after GD treatment were observed by electron microscopy, and the cell cycle changes was analyzed using flow cytometry. The cell apoptosis and mitochondrial potential collapse in the treated cells were tested with Hoechst staining assay and flow cytometry...
September 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Yijian Wu, Huihui Jiang, Erpeng Zhu, Jian Li, Quanxi Wang, Wuduo Zhou, Tao Qin, Xiaoping Wu, Baocheng Wu, Yifan Huang
To elucidate the effect of Hericium erinaceus polysaccharide (HEP) on the intestinal mucosal immunity in normal and Muscovy duck reovirus (MDRV)-infected Muscovy ducklings, 1-day-old healthy Muscovy ducklings were pretreated with 0.2g/L HEP and/or following by MDRV infection in this study, duodenal samples were respectively collected at 1, 3, 6, 10, 15 and 21day post-infection, tissue sections were prepared for observation of morphological structure and determination of intestinal parameters (villus height/crypt depth ratio, villus surface area) as well as counts of intraepithelial lymphocytes (IELs), goblet cells, mast cells...
September 23, 2017: International Journal of Biological Macromolecules
A R O Brito, K Nishinari, P F Saad, K R Saad, M A T Pereira, S C D Emídio, G Yazbek, G A Z Bomfim, R N Cavalcante, M Krutman, M P Teivelis, B S Pignataro, I Y I Fonseca, G Centofanti, B L F Soares
INTRODUCTION: there are only three studies comparing the efficacy of two different types of lock used in totally implantable catheters regarding occlusion or reflux dysfunction. The present study contains the largest published casuistry (862 patients) and is the only that analyzes three parameters: occlusion, reflux dysfunction and flow dysfunction. METHOD: it was a retrospective study of patients operated at a large oncology center and followed up in the outpatient clinic between 2007 and 2015...
September 22, 2017: Annals of Vascular Surgery
Kin Fong Lei, Tai-Kun Liu, Ngan-Ming Tsang
In order to achieve high predictive value of cell chemosensitivity test for clinical efficacy, cancer cells were suggested to be encapsulated and cultured in hydrogel to mimic the natural microenvironment of tumors. However, handling 3D cells/hydrogel culture construct is tedious and cellular response is difficult to be quantitatively analyzed. In the current study, a novel platform for conducting 3D cell culture and analyzing cell viability has been developed towards a high throughput drug screening. Cells encapsulated in the hydrogel were cultured in the microwells of a paper substrate...
September 19, 2017: Biosensors & Bioelectronics
Vladimir Kojecky, Jan Matous, Radan Keil, Milan Dastych, Radek Kroupa, Zdena Zadorova, Michal Varga, Jiri Dolina, Milan Kment, Ales Hep
PURPOSE: The purpose of this study is to compare the efficacy and tolerability of polyethylene glycol (PEG) to sodium picosulfate/magnesium citrate (SPMC) and low-volume polyethylene glycol/ascorbic acid (PEGA) in a single- or split-dose regimen for colonoscopy bowel preparation. METHODS: This was a prospective, randomized, endoscopist-blinded, multicentre study. Outpatients received either PEG or SPMC or PEGA in a single or a split dose before the colonoscopy. Quality and tolerability of the preparation and complaints during preparation were recorded...
September 24, 2017: International Journal of Colorectal Disease
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