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https://www.readbyqxmd.com/read/28530032/hcv-ag-quantification-as-a-one-step-procedure-in-diagnosing-chronic-hepatitis-c-infection-in-cameroon-the-anrs-12336-study
#1
REVIEW
Léa Duchesne, Richard Njouom, Frédéric Lissock, Gishlaine Flore Tamko-Mella, Sandrine Rallier, Lila Poiteau, Alexandre Soulier, Stéphane Chevaliez, Guy Vernet, Nicolas Rouveau, Jean-Michel Pawlotsky, Pierre-Marie Girard, Karine Lacombe
INTRODUCTION: The diagnostic procedure for chronic hepatitis C infection (CHC) usually combines anti-HCV antibody (HCV-Ab) and HCV-RNA measurement. Quantifying HCV core antigen (cAg) as a one-step procedure could shorten the diagnostic process. We aimed to assess the performance of cAg quantification in diagnosing CHC and how it is influenced by concomitant HIV or HBV infections. METHODS: The cAg was quantified by an automated assay (Abbott Diagnostics) in 465 HCV-Ab negative serum samples and 544 HCV-RNA positive serum samples (n = 1009) collected in patients from the Pasteur Center in Cameroon, some of whom were infected by HBV or HIV...
May 15, 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/28524656/multifunctional-and-redox-responsive-self-assembled-magnetic-nanovectors-for-protein-delivery-and-dual-modal-imaging
#2
Hong Yu Yang, Moon-Sun Jang, Yi Li, Jung Hee Lee, Doo Sung Lee
Nanoparticle (NP)-based model carriers present an emerging strategy for protein delivery. However, constructing a multifunctional nanocarrier with high loading capacity, diagnostic imaging capacity and controlled release capability is a tremendous challenge for protein delivery systems. Thus, we herein report on the fabrication of redox-responsive magnetic nanovectors (termed RMNs) through self- assembly of Fe3O4 NPs and redox-responsive polymer ligands, which could effectively transport protein and trigger intracellular protein release...
May 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28521452/assessment-of-high-sensitivity-c-reactive-protein-tests-for-the-diagnosis-of-hepatocellular-carcinoma-in-patients-with-hepatitis-b-associated-liver-cirrhosis
#3
Li-Na Ma, Xiao-Yan Liu, Zhen-Hui Lu, Li-Gang Wu, Yuan-Yuan Tang, Xia Luo, Yan-Chao Hu, Ting-Ting Yan, Qi Wang, Xiang-Chun Ding, Yan Xie
Hepatocellular carcinoma (HCC) is a common malignant tumor worldwide, with high morbidity and mortality. Chronic infection with hepatitis B virus (HBV) is a major risk factor for the development of hepatocellular carcinoma and the majority (~80%) of hepatocellular carcinoma patients in China exhibit co-morbidity with HBV-associated liver cirrhosis. The goal of reliable early diagnostic and prognostic techniques for HBV-associated HCC remains unrealized. The aim of the present study was to explore the efficacy of serum high-sensitivity C-reactive protein (hs-CRP) tests in the early diagnosis of HCC in patients with HBV-associated liver cirrhosis...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521029/soluble-taci-and-soluble-bcma-as-biomarkers-in-primary-central-nervous-system-lymphoma
#4
Franziska S Thaler, Sarah A Laurent, Marion Huber, Matthias Mulazzani, Martin Dreyling, Uwe Ködel, Tania Kümpfel, Andreas Straube, Edgar Meinl, Louisa von Baumgarten
Background: B-cell survival is regulated through interactions of B-cell-activating factor (BAFF) and a proliferation-inducing ligand (APRIL) with their receptors transmembrane activator and CAML interactor (TACI) and B-cell maturation antigen (BCMA). We evaluated the diagnostic potential of soluble TACI (sTACI) and soluble BCMA (sBCMA) in cerebrospinal fluid (CSF) and serum as biomarkers in primary CNS lymphoma (PCNSL). Methods: CSF (n=176) and serum samples (n=105) from patients with clinically or radiologically suspected PCNSL as well as from control patients were collected prospectively...
May 17, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28515032/bilateral-painless-testicular-mass-acute-uveitis-and-annular-cutaneous-lesions-an-unusual-presentation-of-sarcoidosis-and-literature-review
#5
Enver Şimşek, Tülay Şimşek, Meliha Demiral, Funda Canaz, M Fuat Açıkalın
BACKGROUND: Sarcoidosis is a systemic inflammatory disease characterized by non-caseating epitheloid granulomas; whereas it usually involves the lungs and lymph nodes, genitourinary involvement is extremely rare. For this reason, no consensus regarding the diagnostic and management options for testicular sarcoidosis has been reached. OBJECTIVE: The aim of this case report was to describe an unusual clinical presentation of sarcoidosis. An 8-year-old boy presented with a 6-month history of painless scrotal enlargement and a 2-month history of photophobia, blurred vision, and pain in both eyes...
May 17, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28514751/large-scale-clinical-validation-of-biomarkers-for-pancreatic-cancer-using-a-mass-spectrometry-based-proteomics-approach
#6
Jisook Park, Eunjung Lee, Kyoung-Jin Park, Hyung-Doo Park, Jong-Won Kim, Hye In Woo, Kwang Hyuck Lee, Kyu-Taek Lee, Jong Kyun Lee, Joon-Oh Park, Young Suk Park, Jin Seok Heo, Seong Ho Choi, Dong Wook Choi, Kee-Taek Jang, Soo-Youn Lee
We performed an integrated analysis of proteomic and transcriptomic datasets to develop potential diagnostic markers for early pancreatic cancer. In the discovery phase, a multiple reaction monitoring assay of 90 proteins identified by either gene expression analysis or global serum proteome profiling was established and applied to 182 clinical specimens. Nine proteins (P < 0.05) were selected for the independent validation phase and quantified using stable isotope dilution-multiple reaction monitoring-mass spectrometry in 456 specimens...
April 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28514315/role-of-cystatin-c-and-glomerular-filtration-rate-in-diagnosis-of-kidney-impairment-in-hepatic-cirrhosis-patients
#7
Dong Wang, Jia-Fu Feng, An-Qun Wang, Yu-Wei Yang, Yun-Shuang Liu
Hepatic cirrhosis is often accompanied by functional kidney impairment, which may be reversed if early treatment is promptly administered. This study aimed to investigate the role of Cystatin C and Cystatin C estimated glomerular filtration rate in the diagnosis of kidney impairment in patients with hepatic cirrhosis.Four hundred sixty five patients with hepatic cirrhosis were recruited. Serum creatinine and Cystatin C were determined, and their estimated glomerular filtration rates were calculated.The area under the receiver-operating characteristic curve (area under curve [AUC]) of Cystatin C and Cystatin C estimated glomerular filtration rate was significantly larger than that of serum creatinine and serum creatinine estimated glomerular filtration rate, respectively (P = ...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28513463/alzheimer-s-disease-against-peptides-products-of-enzymatic-cleavage-app-protein-biological-pathobiological-and-physico-chemical-properties-of-fibrillating-peptides
#8
Małgorzata Marszałek
Various peptides products of enzymatic cleavage of key for Alzheimer's disease Amyloid Precursor Protein (APP) are well known, but still are matter of scientific debate. The Aβ type products are especially challenging for experimental and medical research. This paper outlines several, still poorly known, biological and medical processes such as peptides biology, i.e., formation, biodistribution, translocation, transport and finally removal from brain compartments and body fluids like Intracellular Fluid (ICF), Cerebrospinal Fluid (CSF), Interstitial Fluid (ISF), blood serum or urine...
May 17, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28513288/growth-arrest-specific-protein-6-gas6-as-a-noninvasive-biomarker-for-early-detection-of-diabetic-nephropathy
#9
Wenni Li, Junlin Wang, Lina Ge, Jiehui Shan, Chunbing Zhang, Jianping Liu
BACKGROUND: To investigate the diagnostic level of cystatin C and growth arrest-specific gene 6 (Gas6) levels in elderly type 2 diabetic patients with different degrees of diabetic nephropathy (DN). METHODS: Four hundred and eighty-two old people, including 130 healthy controls, 130 normoalbuminuric diabetic patients, 122 with microalbuminuria, and 100 with macroalbuminuria, were recruited. Plasma Gas6 and serum cystatin C levels were measured. RESULTS: Plasma Gas6 concentration was significantly lower in diabetic patients with microalbuminuria or macroalbuminuria, as compared with diabetic subjects with normoalbuminuria; while cystatin C was significantly higher...
May 17, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28511949/serum-metal-levels-for-diagnosis-of-adverse-local-tissue-reactions%C3%A2-secondary-to-corrosion-in-metal-on-polyethylene-total%C3%A2-hip%C3%A2-arthroplasty
#10
Yale A Fillingham, Craig J Della Valle, Daniel D Bohl, Mick P Kelly, Deborah J Hall, Robin Pourzal, Joshua J Jacobs
BACKGROUND: Recently, corrosion at the head-neck junction in metal-on-polyethylene bearing surface total hip arthroplasty (THA) has been recognized as a cause of adverse local tissue reactions (ALTRs). Serum metal levels have been advocated as a tool for the diagnosis of ALTR; however, no prior studies have specifically examined their utility. The purpose of this study was to determine the optimal cutoff values for serum cobalt and chromium levels in diagnosing ALTR after metal-on-polyethylene bearing surface THA...
April 20, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28510335/proteoforms-in-peripheral-blood-mononuclear-cells-as-novel-rejection-biomarkers-in-liver-transplant-recipients
#11
T K Toby, M Abecassis, K Kim, P M Thomas, R T Fellers, R D LeDuc, N L Kelleher, J Demetris, J Levitsky
Biomarker profiles of acute rejection in liver transplant recipients could enhance the diagnosis and management of recipients. Our aim was to identify diagnostic proteoform signatures of acute rejection in circulating immune cells, using an emergent "top down" proteomics methodology. We prepared differentially processed and cryopreserved cell lysates from 26 non-viral liver transplant recipients by molecular weight-based fractionation and analyzed them by mass spectrometry of whole proteins in three steps: 1) Nanocapillary liquid chromatography coupled with high-resolution tandem mass spectrometry; 2) database searching to identify and characterize intact proteoforms; 3) data processing through a hierarchical linear model matching the study design to quantify proteoform fold changes in patients with rejection vs...
May 16, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28507919/hepatitis-c-virus-rna-strands-detection-in-peripheral-blood-mononuclear-cells-legitimizes-virus-eradication-in-negative-serum-pcr-na%C3%A3-ve-and-post-treatment-patients
#12
Mohamed Darwish Ahmed Abd Alla, Mostafa Kamel El Awady
Background and Aims: Hepatitis C virus (HCV) hepatotropism is associated with intra-peripheral blood mononuclear cell (PBMC) infection that causes post-treatment relapse in RNA seronegative patients. Our understanding of the association of non-viremic hepatic fibrosis with positive anti-HCV IgG antibodies and active hepatocellular damage might be increased by PBMCs screening for intracellular infection. Thus, the goals of this study included evaluation of PBMCs PCR for diagnosing HCV infection, addressing PBMCs plus serum real-time (SRT) PCR benefits over SRT-PCR alone, studying intra-PBMCs distribution of RNA sense and antisense strands, and identifying treatment feasibility in solitary intracellular infection...
March 28, 2017: Journal of Clinical and Translational Hepatology
https://www.readbyqxmd.com/read/28507579/assessment-of-concentrations-of-strail-ligand-and-its-receptors-strail-r1-and-strail-r2-markers-monitoring-the-course-of-the-extrinsic-pathway-of-apoptosis-induction-potential-application-in-ovarian-cancer-diagnostics
#13
Aleksandra Mielczarek-Palacz, Justyna Sikora, Zdzisława Kondera-Anasz
INTRODUCTION: TNF-related apoptosis-inducing ligand (TRAIL) together with its receptors are involved in activation of the extrinsic pathway of apoptosis. Due to the special role of the apoptosis pathway in pathogenesis of ovarian cancers, the aim of the study was to assess concentrations of sTRAIL, sTRAIL-R1 and sTRAIL-R2 in serum of affected women. MATERIAL AND METHODS: The study group included 85 women with diagnosed ovarian tumors: 35 women with ovarian serous cystadenoma, 15 women with ovarian teratoma and 35 women with serous cystadenocarcinoma...
April 1, 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28507202/determination-of-volatile-organic-compounds-exhaled-by-cell-lines-derived-from-hematological-malignancies
#14
Hongxia Tang, Yan Lu, Lulu Zhang, Zhonghui Wu, Xiaofang Hou, Hailong Xia
Background: The gas human exhaled contains many volatile organic compounds (VOCs), which is related to the health status of body. Analysis of VOCs has been proposed as a noninvasive diagnostic tool for certain cancers. Detailed research on the VOCs in gas exhaled by cell can characterize cell type specific metabolites and may be helpful to detect the cancer markers in clinical practice. Methods: Solid phase microextraction-gas chromatography-mass spectrometry was used to detect VOCs in the headspace of tissue culture flask in non-Hodgkin's lymphoma cell line JEKO and acute mononuclear leukemia cell line SHI-1, to elaborate the characteristic gaseous biomarkers of hematological malignancies...
May 15, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28506795/parallel-elisas-using-crude-soluble-antigen-and-excretory-secretory-antigen-for-improved-serodiagnosis-of-amoebic-liver-abscess
#15
Weng-Kin Wong, Phiaw-Chong Foo, Alfonso Olivos-Garcia, Rahmah Noordin, Zeehaida Mohamed, Nurulhasanah Othman, Ling-Ling Few, Boon-Huat Lim
Crude soluble antigen (CSA) produced from Entamoeba histolytica trophozoite is conventionally used for serodiagnosis of invasive amoebiasis. However, high background seropositivities by CSA-assay in endemic areas complicate the interpretation of positive result in clinical settings. Instead, incorporating a second assay which indicates active or recent infection into the routine amoebic serology could possibly complement the limitations of CSA-assay. Hence, the present study aimed to evaluate the diagnostic efficacies of indirect ELISAs using CSA and excretory-secretory antigen (ESA) for serodiagnosis of amoebic liver abscess (ALA)...
May 12, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28506782/the-misleading-effect-of-serum-galactomannan-testing-in-high-risk-hematology-patients-receiving-prophylaxis-with-micafungin
#16
A Vena, E Bouza, A Álvarez-Uría, J Gayoso, P Martin-Rabadan, F Cajuste, J Guinea, J Gómez Castellá, R Alonso, P Munoz
OBJECTIVES: To investigate the performance of the routine serum galactomannan (sGM) assay in the diagnosis of invasive aspergillosis (IA) in high-risk hematology patients receiving prophylaxis with micafungin. METHODS: Retrospective study including all hematological patients who received prophylaxis with micafungin during high-risk IA episodes (neutropenic patients after chemotherapy for acute myeloid leukaemia/myelodysplastic syndrome; allogeneic HSCT during early neutropenic phase or graft-versus-host disease requiring high prednisone doses)...
May 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28506369/autoimmune-hepatitis-overlap-syndromes-and-liver-pathology
#17
REVIEW
Albert J Czaja, Herschel A Carpenter
Autoimmune hepatitis (AIH) may have an atypical serum alkaline phosphatase elevation, antimitochondrial antibodies, histologic features of bile duct injury/loss, or cholangiographic findings of focal biliary strictures and dilations. These manifestations characterize the overlap syndromes. Patients can be classified as having AIH with features of primary biliary cholangitis, primary sclerosing cholangitis, or a cholestatic syndrome. The gold standard of diagnosis is clinical judgment. Histologic evaluation is a major diagnostic component...
June 2017: Gastroenterology Clinics of North America
https://www.readbyqxmd.com/read/28505570/clinical-evaluation-of-the-biofire-filmarray-%C3%A2-biothreat-e-test-for-the-diagnosis-of-ebola-virus-disease-in-guinea
#18
Françoise Gay-Andrieu, N'Fally Magassouba, Valentina Picot, Cynthia L Phillips, Christophe N Peyrefitte, Brigitte Dacosta, Ahmadou Doré, Fode Kourouma, Véronique Ligeon-Ligeonnet, Corentin Gauby, Christophe Longuet, Matt Scullion, Ousmane Faye, Jean Louis Machuron, Mark Miller
BACKGROUND: The recent West Africa Ebola outbreak highlighted the need to provide access to rapid, safe and reliable Ebola Virus Disease diagnostics. OBJECTIVES: The objective of this field study was to assess the clinical performance of the FilmArray(®) BioThreat-E test for the detection of Ebola Zaïre virus in whole blood in symptomatic patients suspected of Ebola Virus Disease in Conakry (Guinea) from March to July 2015. STUDY DESIGN: The BioThreat-E test was compared to the two RT-PCRs, using serum, implemented at Donka Hospital in the emergency context: an in-house developed quantitative one-step RT-PCR adapted from the Weidmann technique, and the RealStar(®) Filovirus RT-PCR Kit 1...
May 1, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28503423/microrna-126-a-promising-novel-biomarker-in-peripheral-blood-for-diabetic-retinopathy
#19
Li-Li Qin, Mei-Xia An, Yan-Li Liu, Han-Chun Xu, Zhi-Qing Lu
AIM: To investigate the content of serum microRNA-126 (miR-126) and its role in screening retinal endothelial injury and early diagnosis of proliferative diabetic retinopathy. METHODS: The study included 184 serum samples, 59 samples from healthy individuals, 44 samples from diabetes mellitus (DM) patients without diabetic retinopathy (NDR), 42 from non-proliferative diabetic retinopathy (NPDR) patients and 39 samples from proliferative diabetic retinopathy (PDR) patients...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28503282/skeletal-complications-of-brucellosis-a-study-of-464-cases-in-babol-iran
#20
Soheil Ebrahimpour, Masomeh Bayani, Zahra Moulana, Mohammad Reza Hasanjani Roushan
BACKGROUND: Skeletal complication of brucellosis is common in endemic region of brucellosis, but its frequency has not been clearly determined. The aim of this study was to determine the frequency of skeletal complication in brucellosis patients in Babol, north of Iran. METHODS: From 2005-2015, 1252 cases of brucellosis were diagnosed at the Department of Infectious Diseases, Ayatollah Rouhani Teaching Hospital, in Babol, North of Iran. The diagnosis of brucellosis was established using serum agglutination test (SAT≥1/160), and 2-mercaptoethanol (2ME≥ 1/80) with clinical and epidemiological findings compatible with brucellosis...
2017: Caspian Journal of Internal Medicine
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