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https://www.readbyqxmd.com/read/28442219/virulence-potential-of-staphylococcus-aureus-isolates-from-buruli-ulcer-patients
#1
Nana Ama Amissah, Monika A Chlebowicz, Anthony Ablordey, Caitlin S Tetteh, Isaac Prah, Tjip S van der Werf, Alex W Friedrich, Jan Maarten van Dijl, Ymkje Stienstra, John W Rossen
Buruli ulcer (BU) is a necrotizing infection of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. BU wounds may also be colonized with other microorganisms including Staphylococcus aureus. This study aimed to characterize the virulence factors of S. aureus isolated from BU patients. Previously sequenced genomes of 21 S. aureus isolates from BU patients were screened for the presence of virulence genes. The results show that all S. aureus isolates harbored on their core genomes genes for known virulence factors like α-hemolysin, and the α- and β-phenol soluble modulins...
April 19, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28441421/high-prevalence-of-mrsa-and-esbl-among-asylum-seekers-in-the-netherlands
#2
Sofanne J Ravensbergen, Matthijs Berends, Ymkje Stienstra, Alewijn Ott
Migration is one of the risk factors for the spread of multidrug-resistant organisms (MDRO). The increasing influx of migrants challenges local health care systems. To provide evidence for both hospital hygiene measure and empirical antibiotic therapy, we analysed all cultures performed in asylum seekers between January 1st 2014 and December 31st 2015 for methicillin resistant Staphylococcus aureus (MRSA) and for multidrug-resistant Enterobacteriaceae (MDRE). We compared these with cultures from the Dutch patient population with risk factors for carriage of MDRO...
2017: PloS One
https://www.readbyqxmd.com/read/28409124/hospital-dissemination-of-tst-1-positive-clonal-complex-5-cc5-methicillin-resistant-staphylococcus-aureus
#3
Min Wang, Yi Zheng, Jose R Mediavilla, Liang Chen, Barry N Kreiswirth, Yajun Song, Ruifu Yang, Hong Du
Methicillin-resistant Staphylococcus aureus (MRSA), is one of the most prevalent clinical pathogens isolated from hospital settings, and has increasingly identified in community settings. In China, the SCCmecIII-ST239 strains are disseminated in different geographic regions, accounting for >75% of all MRSA isolates in some national studies. Here we characterized 150 non-duplicate MRSA isolates collected from February 2012 to May 2013 in a tertiary hospital in Suzhou, Eastern China, to explore the molecular epidemiology...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28407796/prevalence-of-methicillin-resistant-staphylococcus-aureus-mrsa-infection-and-the-molecular-characteristics-of-mrsa-bacteraemia-over-a-two-year-period-in-a-tertiary-teaching-hospital-in-malaysia
#4
Pik San Sit, Cindy Shuan Ju Teh, Nuryana Idris, I-Ching Sam, Sharifah Faridah Syed Omar, Helmi Sulaiman, Kwai Lin Thong, Adeeba Kamarulzaman, Sasheela Ponnampalavanar
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is an established pathogen that causes hospital- and community-acquired infections worldwide. The prevalence rate of MRSA infections were reported to be the highest in Asia. As there is limited epidemiological study being done in Malaysia, this study aimed to determine the prevalence of MRSA infection and the molecular characteristics of MRSA bacteraemia. METHODS: Two hundred and nine MRSA strains from year 2011 to 2012 were collected from a tertiary teaching hospital in Malaysia...
April 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28400021/east-and-west-african-milk-products-are-reservoirs-for-human-and-livestock-associated-staphylococcus-aureus
#5
Christoph Jans, Axel Merz, Sophia Johler, Mario Younan, Sabine A Tanner, Dasel Wambua Mulwa Kaindi, John Wangoh, Bassirou Bonfoh, Leo Meile, Taurai Tasara
Staphylococcus aureus frequently isolated from milk products in sub-Saharan Africa (SSA) is a major pathogen responsible for food intoxication, human and animal diseases. SSA hospital-derived strains are well studied but data on the population structure of foodborne S. aureus required to identify possible staphylococcal food poisoning sources is lacking. Therefore, the aim was to assess the population genetic structure, virulence and antibiotic resistance genes associated with milk-derived S. aureus isolates from Côte d'Ivoire, Kenya and Somalia through spa-typing, MLST, and DNA microarray analysis...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399994/prevalence-and-molecular-characterization-of-staphylococcus-aureus-in-commercially-available-meat-over-a-one-year-period-in-iowa-usa
#6
Dipendra Thapaliya, Brett M Forshey, Jhalka Kadariya, Megan K Quick, Sarah Farina, Ashley O' Brien, Rajeshwari Nair, Amos Nworie, Blake Hanson, Ashley Kates, Shylo Wardyn, Tara C Smith
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of infectious disease morbidity and mortality. Previous studies have confirmed the presence of S. aureus, including MRSA, on raw meat products. We investigated the prevalence and molecular epidemiology of S. aureus and MRSA in commercially-distributed antibiotic-free and conventional raw meat products (n = 3290) purchased in 8 Iowa retail stores weekly for a period of one year. Isolates were characterized using spa typing, and PCR was used to detect the presence of the Panton-Valentine leukocidin (PVL) and mecA genes...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28357840/possible-outbreak-of-skin-infections-due-to-panton-valentine-leukocidin-positive-methicillin-resistant-staphylococcus-aureus-on-a-commercial-ship-in-2012-2014
#7
Etienne Bon, Kheira Gadouri, Catherine Avril, Guillaume Camuset, Elsa Balleydier, Isabelle Mathieu, Sandrine Picot, Julien Jaubert, Richard Ballas, Mélanie Colomb-Cotinat, Anne Tristan, Coralie Bouchiat, Laurent Filleul, Frédéric Pagès
No abstract text is available yet for this article.
2017: International Maritime Health
https://www.readbyqxmd.com/read/28336116/purulent-pericarditis-by-staphylococcus-panton-valentine-secondary-to-hair-implants
#8
Leydimar Anmad Shihadeh, Patricia Couto Comba, Carolina Hernández Carballo
No abstract text is available yet for this article.
March 20, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28283923/characterization-of-staphylococcus-aureus-in-goose-feces-from-state-parks-in-northeast-ohio
#9
Dipendra Thapaliya, Mark Dalman, Jhalka Kadariya, Katie Little, Victoria Mansell, Mohammed Y Taha, Dylan Grenier, Tara C Smith
Staphylococcus aureus can colonize a range of species. Although numerous studies have isolated pathogenic bacteria from wild birds, very little is known regarding S. aureus and their potential to spread methicillin-resistant (MRSA) strains. The objective of this study was to determine the presence and molecular characteristics of S. aureus in geese fecal samples collected from ten state parks across Northeast Ohio (NEO). A total of 182 fecal samples from Canada geese (Branta canadensis) were collected in April 2015...
March 10, 2017: EcoHealth
https://www.readbyqxmd.com/read/28273954/community-associated-staphylococcus-aureus-from-sub-saharan-africa-and-germany-a-cross-sectional-geographic-correlation-study
#10
Ulla Ruffing, Abraham Alabi, Theckla Kazimoto, Delfino C Vubil, Ruslan Akulenko, Salim Abdulla, Pedro Alonso, Markus Bischoff, Anja Germann, Martin P Grobusch, Volkhard Helms, Jonas Hoffmann, Winfried V Kern, Peter G Kremsner, Inacio Mandomando, Alexander Mellmann, Georg Peters, Frieder Schaumburg, Sabine Schubert, Lena Strauß, Marcel Tanner, Hagen von Briesen, Laura Wende, Lutz von Müller, Mathias Herrmann
Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease and are well characterized in industrialized countries but poorly analysed in developing regions. The objective of this study was to compare the molecular-epidemiologic profiles of S. aureus isolates from Sub-Saharan Africa and Germany. S. aureus isolates from 600 staphylococcal carriers and 600 patients with community-associated staphylococcal disease were characterized by DNA hybridization, clonal complex (CC) attribution, and principal component (PCA)-based gene repertoire analysis...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28259072/a-rare-case-of-acute-osteomyelitis-due-to-panton-valentine-leukocidin-positive-community-acquired-methicillin-resistant-staphylococcus-aureus-in-a-young-healthy-adult
#11
Toshiyuki Takemori, Osamu Nakamura, Yoshiki Yamagami, Hideki Nishimura, Teruya Kawamoto, Toshihiro Akisue, Tetsuji Yamamoto
INTRODUCTION: Most community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections affect skin or soft tissues, while invasive and life-threatening illnesses including osteomyelitis are less common. CA-MRSA infections occur especially in the pediatric age group, while the occurrence of CA-MRSA osteomyelitis in adults is uncommonly reported. PRESENTATION OF CASES: A rare case of acute osteomyelitis of the femur caused by Panton-Valentine leukocidin (PVL)-positive CA-MRSA in a 37-year-old man in good health is presented...
February 17, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28251179/fluorocycline-tp-271-is-potent-against-complicated-community-acquired-bacterial-pneumonia-pathogens
#12
Trudy H Grossman, Corey Fyfe, William O'Brien, Meredith Hackel, Mary Beth Minyard, Ken B Waites, Jacques Dubois, Timothy M Murphy, Andrew M Slee, William J Weiss, Joyce A Sutcliffe
TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0...
January 2017: MSphere
https://www.readbyqxmd.com/read/28210352/risk-of-skin-and-soft-tissue-infections-among-children-found-to-be-staphylococcus-aureus-mrsa-usa300-carriers
#13
Lilly Cheng Immergluck, Shabnam Jain, Susan M Ray, Robert Mayberry, Sarah Satola, Trisha Chan Parker, Keming Yuan, Anaam Mohammed, Robert C Jerris
INTRODUCTION: The purpose of this study was to examine community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) carriage and infections and determine risk factors associated specifically with MRSA USA300. METHODS: We conducted a case control study in a pediatric emergency department. Nasal and axillary swabs were collected, and participants were interviewed for risk factors. The primary outcome was the proportion of S. aureus carriers among those presenting with and without a skin and soft tissue infection (SSTI)...
February 2017: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28184301/detection-of-diverse-genotypes-of-methicillin-resistant-staphylococcus-aureus-from-hospital-personnel-and-the-environment-in-armenia
#14
Hermine V Mkrtchyan, Zhen Xu, Maria Yacoub, Mary M Ter-Stepanyan, Hayk D Karapetyan, Angela M Kearns, Ronald R Cutler, Bruno Pichon, Armen Dz Hambardzumyan
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a public health concern internationally. Studies examining a range of cohorts have been reported from various regions of the world, but little is known about the molecular epidemiology of MRSA in Armenia. METHODS: Between May and September 2013, twenty isolates of methicillin-resistant Staphylococcus aureus (MRSA; mecA positive) were recovered from hospital personnel (n = 10; 9 females, 1 male) and environmental sites (n = 10) in the maternity ward of one of the teaching hospitals in Armenia...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28146185/-community-acquired-staphylococcus-aureus-isolated-from-paraguayan-children-clinical-phenotypic-and-genotypic-characterization
#15
Rosa Guillén, Letizia Carpinelli, Fátima Rodríguez, Héctor Castro, Beatriz Quíñónez, Ana Campuzano, María Macchi, Juana Ortellado, Patricia Almada, Lorena Grau, Mónica Rodríguez, Gladys Velázquez, Carmen Espínola, Gloría Samudio, Gloria Gómez, Wilma Basualdo
INTRODUCTION: The prevalence of Staphylococcus aureus in the community has increased, being the pediatric population the most affected. This fact highlights the need for epidemiological surveillance. AIM: To characterize clinical, phenotypic and genotypic isolates of S. aureus children's samples with community-acquired infections, collected in hospitals of Asuncion and the Central Department, between November 2009 and December 2010. MATERIALS AND METHODS: Descriptive and transverse analysis with analytical component...
December 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28138384/hospital-clones-of-methicillin-resistant-staphylococcus-aureus-are-carried-by-medical-students-even-before-healthcare-exposure
#16
Ido Orlin, Assaf Rokney, Avi Onn, Daniel Glikman, Avi Peretz
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) strains are prevalent in healthcare and the community. Few studies have examined MRSA carriage among medical students. The aim of this study is to examine Staphylococcus aureus (SA) carriage, and particular MRSA, over time in cohort medical students. METHODS: Prospective collection of nasal swabs from medical students in Israel and assessment of SA carriage. Three samples were taken per student in preclinical and clinical parts of studies...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28137816/effects-of-tedizolid-phosphate-on-survival-outcomes-and-suppression-of-production-of-staphylococcal-toxins-in-a-rabbit-model-of-methicillin-resistant-staphylococcus-aureus-necrotizing-pneumonia
#17
Vien T M Le, Hoan N Le, Marcos Gabriel Pinheiro, Kenneth J Hahn, Mary L Dinh, Kajal B Larson, Shawn D Flanagan, Cedric Badiou, Gerard Lina, Christine Tkaczyk, Bret R Sellman, Binh An Diep
The protective efficacy of tedizolid phosphate, a novel oxazolidinone that potently inhibits bacterial protein synthesis, was compared to those of linezolid, vancomycin, and saline in a rabbit model of Staphylococcus aureus necrotizing pneumonia. Tedizolid phosphate was administered to rabbits at 6 mg/kg of body weight intravenously twice daily, which yielded values of the 24-h area under the concentration-time curve approximating those found in humans. The overall survival rate was 83% for rabbits treated with 6 mg/kg tedizolid phosphate twice daily and 83% for those treated with 50 mg/kg linezolid thrice daily (P = 0...
April 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28131643/bengal-bay-clone-st772-mrsa-v-outbreak-conserved-clone-causes-investigation-challenges
#18
COMPARATIVE STUDY
A Blomfeldt, K W Larssen, A Moghen, K Haugum, T W Steen, S B Jørgensen, H V Aamot
BACKGROUND: The Bengal Bay clone, ST772-MRSA-V, associated with multi-drug resistance, Panton-Valentine leukocidin (PVL) and skin and soft tissue infections, is emerging worldwide. In Norway, a country with low prevalence of meticillin-resistant Staphylococcus aureus (MRSA), increased occurrence of ST772-MRSA-V has also caused hospital outbreaks. The conserved nature of this clone challenged the outbreak investigations. AIM: To evaluate the usefulness of S. aureus protein A (spa) typing, multiple-locus variable number tandem repeat fingerprinting/analysis (MLVF/MLVA) and pulsed-field gel electrophoresis (PFGE) when investigating outbreaks with a conserved MRSA clone...
March 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28117704/above-and-beyond-c5a-receptor-targeting-by-staphylococcal-leucotoxins-retrograde-transport-of-panton-valentine-leucocidin-and-%C3%AE-hemolysin
#19
Gaëlle Zimmermann-Meisse, Gilles Prévost, Emmanuel Jover
Various membrane receptors associated with the innate immune response have recently been identified as mediators of the cellular action of Staphylococcus aureus leucotoxins. Two of these, the Panton-Valentine leucotoxin LukS-PV/LukF-PV and the γ-hemolysin HlgC/HlgB, bind the C5a complement-derived peptide receptor. These leucotoxins utilize the receptor to induce intracellular Ca(2+) release from internal stores, other than those activated by C5a. The two leucotoxins are internalized with the phosphorylated receptor, but it is unknown whether they divert retrograde transport of the receptor or follow another pathway...
January 20, 2017: Toxins
https://www.readbyqxmd.com/read/28116552/characterisation-of-staphylococcus-aureus-isolates-from-bloodstream-infections-democratic-republic-of-the-congo
#20
S Vandendriessche, H De Boeck, A Deplano, M-F Phoba, O Lunguya, D Falay, N Dauly, J Verhaegen, O Denis, J Jacobs
Staphylococcus aureus is known worldwide as an invasive pathogen, but information on S. aureus from bloodstream infections in Central Africa remains scarce. A collection of S. aureus blood culture isolates recovered from hospitals in four provinces in the Democratic Republic of the Congo (2009-2013) was assessed. A total of 27/108 isolates were methicillin-resistant S. aureus (MRSA), of which >70% were co-resistant to aminoglycosides, tetracyclines, macrolides and lincosamides. For MRSA and methicillin-susceptible S...
January 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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