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Loxoscelism

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https://www.readbyqxmd.com/read/28264436/toxin-fused-with-sumo-tag-a-new-expression-vector-strategy-to-obtain-recombinant-venom-toxins-with-easy-tag-removal-inside-the-bacteria
#1
Lhiri H A L Shimokawa-Falcão, Maria C Caporrino, Katia C Barbaro, Maisa S Della-Casa, Geraldo S Magalhães
Many animal toxins may target the same molecules that need to be controlled in certain pathologies; therefore, some toxins have led to the formulation of drugs that are presently used, and many other drugs are still under development. Nevertheless, collecting sufficient toxins from the original source might be a limiting factor in studying their biological activities. Thus, molecular biology techniques have been applied in order to obtain large amounts of recombinant toxins into Escherichia coli. However, most animal toxins are difficult to express in this system, which results in insoluble, misfolded, or unstable proteins...
February 27, 2017: Toxins
https://www.readbyqxmd.com/read/28257106/tetracycline-reduces-kidney-damage-induced-by-loxosceles-spider-venom
#2
Cinthya Kimori Okamoto, Carmen W van den Berg, Mizuno Masashi, Rute M Gonçalves-de-Andrade, Denise V Tambourgi
Envenomation by Loxosceles spider can result in two clinical manifestations: cutaneous and systemic loxoscelism, the latter of which includes renal failure. Although incidence of renal failure is low, it is the main cause of death, occurring mainly in children. The sphingomyelinase D (SMase D) is the main component in Loxosceles spider venom responsible for local and systemic manifestations. This study aimed to investigate the toxicity of L. intermedia venom and SMase D on kidney cells, using both In vitro and in vivo models, and the possible involvement of endogenous metalloproteinases (MMP)...
March 2, 2017: Toxins
https://www.readbyqxmd.com/read/28194160/highlights-in-the-knowledge-of-brown-spider-toxins
#3
REVIEW
Daniele Chaves-Moreira, Andrea Senff-Ribeiro, Ana Carolina Martins Wille, Luiza Helena Gremski, Olga Meiri Chaim, Silvio Sanches Veiga
Brown spiders are venomous arthropods that use their venom for predation and defense. In humans, bites of these animals provoke injuries including dermonecrosis with gravitational spread of lesions, hematological abnormalities and impaired renal function. The signs and symptoms observed following a brown spider bite are called loxoscelism. Brown spider venom is a complex mixture of toxins enriched in low molecular mass proteins (4-40 kDa). Characterization of the venom confirmed the presence of three highly expressed protein classes: phospholipases D, metalloproteases (astacins) and insecticidal peptides (knottins)...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28146203/-loxoscelism-and-changes-in-coagulation-profile
#4
Rafael Pichardo-Rodríguez, J Antonio Grandez-Urbina
No abstract text is available yet for this article.
December 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28038994/cutaneous-loxoscelism-extensive-nonulcerated-livedoid-plaque
#5
Natalie Torres, Monica Rueda
No abstract text is available yet for this article.
December 27, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27846029/nonhealing-wounds-caused-by-brown-spider-bites-application-of-hyperbaric-oxygen-therapy
#6
Amir Hadanny, Gregory Fishlev, Yair Bechor, Oshra Meir, Shai Efrati
BACKGROUND: Bites by Loxosceles spiders (also known as recluse spiders or brown spiders) can cause necrotic ulcerations of various sizes and dimensions. The current standard of care for brown spider bites includes analgesics, ice, compression, elevation, antihistamines, and surgical debridement. Hyperbaric oxygen therapy (HBOT) in the treatment of brown spider bites has been administered in the early stage of ulceration, or 2 to 6 days after the bite. Unfortunately, the diagnosis of spider bite-related ulcers is often delayed and weeks or months may elapse before HBOT is considered...
December 2016: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/27743460/insecticidal-activity-of-a-recombinant-knottin-peptide-from-loxosceles-intermedia-venom-and-recognition-of-these-peptides-as-a-conserved-family-in-the-genus
#7
F H Matsubara, G O Meissner, V Herzig, H C Justa, B C L Dias, D Trevisan-Silva, L H Gremski, W Gremski, A Senff-Ribeiro, O M Chaim, G F King, S S Veiga
Loxosceles intermedia venom comprises a complex mixture of proteins, glycoproteins and low molecular mass peptides that act synergistically to immobilize envenomed prey. Analysis of a venom-gland transcriptome from L. intermedia revealed that knottins, also known as inhibitor cystine knot peptides, are the most abundant class of toxins expressed in this species. Knottin peptides contain a particular arrangement of intramolecular disulphide bonds, and these peptides typically act upon ion channels or receptors in the insect nervous system, triggering paralysis or other lethal effects...
October 15, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27692235/-cutaneous-loxoscelism-about-an-exceptional-observation-of-9-consecutive-cases
#8
F Boissiere, R Masson, S Fluieraru, J Vitse, L Dessena, M Lefevre, F Bekara, C Herlin
INTRODUCTION: Loxosceles spiders are ubiquitous and responsible for many cases of envenomation in the world. The kind rufescens is present in the Provence and Occitan regions in France. During the summer 2015, we faced many Loxosceles rufescens cases of bites having led to extensive integumental necrosis whose features and singular evolution seems important to report. MATERIAL AND METHODS: We report the cases of nine patients who experienced a spider bite in the summer of 2015 in the Languedoc Roussillon...
December 2016: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/27651958/spider-bite-a-rare-case-of-acute-necrotic-arachnidism-with-rapid-and-fatal-evolution
#9
Mario Pezzi, Anna Maria Giglio, Annamaria Scozzafava, Orazio Filippelli, Giuseppe Serafino, Mario Verre
The spider bites are quite frequent and often resolve quickly without leaving outcomes; only some species are capable of causing necrotic and systematic lesions in humans. Among them, we should mention the genus Loxosceles. The venom released from the spider bite of Loxosceles species is composed of proteins, enzymes, and nonenzymatic polypeptides. The phospholipase D family was identified as the active component of the venom. This family of enzymes is responsible for the local and systemic effects observed in loxoscelism...
2016: Case Reports in Emergency Medicine
https://www.readbyqxmd.com/read/27579754/acute-generalized-exanthematous-pustulosis-associated-with-spider-bite
#10
Laura de Mattos Milman, Giana Paula Müller, Paulo Ricardo Martins Souza, Aline Barcellos Grill, Deise Louise Bohn Rhoden, Carlos Augusto Mello-da-Silva, Gerson Vettorato
Acute generalized exanthematous pustulosis (AGEP) is an acute febrile rash, usually induced by drugs, which recently has been linked to spider bite. We report a case of a male patient, 48 years old, with an erythematous rash accompanied by fever and small non-follicular pustules. He reported previous pain in the buttock with the onset of a necrotic plaque. The lesion was compatible with spider bite of the genus Loxosceles. According to the EuroSCAR group instrument, the patient scored +10 indicating definite diagnosis of AGEP...
July 2016: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/27563734/potential-implications-for-designing-drugs-against-the-brown-spider-venom-phospholipase-d
#11
Daniele Chaves-Moreira, Fábio Rogério de Moraes, Ícaro Putinhon Caruso, Olga Meiri Chaim, Andrea Senff-Ribeiro, Anwar Ullah, Luciane Sussuchi da Silva, Jorge Chahine, Raghuvir K Arni, Silvio Sanches Veiga
Loxoscelism refers to the clinical symptoms that develop after brown spider bites. Brown spider venoms contain several phospholipase-D isoforms, which are the main toxins responsible for both the cutaneous and systemic effects of loxoscelism. Understanding of the phospholipase-D catalytic mechanism is crucial for the development of specific treatment that could reverse the toxic effects caused by the spider bite. Based on enzymatic, biological, structural, and thermodynamic tests, we show some features suitable for designing drugs against loxoscelism...
August 26, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27496061/description-of-loxtox-protein-family-and-identification-of-a-new-group-of-phospholipases-d-from-loxosceles-similis-venom-gland
#12
Arthur Estanislau Dantas, A O Carmo, Carolina Campolina Rebello Horta, Hortênsia Gomes Leal, Bárbara Bruna Ribeiro Oliveira-Mendes, Ana Paula Vimieiro Martins, Carlos Chávez-Olórtegui, Evanguedes Kalapothakis
Envenoming resulting from Loxosceles spider bites (loxoscelism) is a recognized public health problem in Brazil. However, the pathophysiology of loxoscelism caused by L. similis bites, which is widespread in Brazil, remains poorly understood. In the present work, the RNA sequencing (RNA-Seq - Next Generation sequencing - NGS) of the L. similis venom gland was performed to identify and analyze the sequences of the key component phospholipase D. The sequences were aligned based on their classical domains, and a phylogenetic tree was constructed...
September 15, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27488102/molecular-cloning-and-in-silico-characterization-of-knottin-peptide-u2-scrtx-lit2-from-brown-spider-loxosceles-intermedia-venom-glands
#13
Gabriel Otto Meissner, Pedro Túlio de Resende Lara, Luis Paulo Barbour Scott, Antônio Sérgio Kimus Braz, Daniele Chaves-Moreira, Fernando Hitomi Matsubara, Eduardo Mendonça Soares, Dilza Trevisan-Silva, Luiza Helena Gremski, Silvio Sanches Veiga, Olga Meiri Chaim
Inhibitor cystine knots (ICKs) are a family of structural peptides with a large number of cysteine residues that form intramolecular disulfide bonds, resulting in a knot. These peptides are involved in a variety of biological functions including predation and defense, and are found in various species, such as spiders, scorpions, sea anemones, and plants. The Loxosceles intermedia venom gland transcriptome identified five groups of ICK peptides that represent more than 50 % of toxin-coding transcripts. Here, we describe the molecular cloning of U2-Sicaritoxin-Lit2 (U2-SCRTX-Lit2), bioinformatic characterization, structure prediction, and molecular dynamic analysis...
September 2016: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/27440851/rare-case-of-dermonecrosis-caused-by-a-recluse-spider-bite-in-europe
#14
Monique Cachia, Liam Mercieca, Charles Mallia Azzopardi, Michael J Boffa
Spider poisoning is rare in Europe, with very few reported cases in the literature. Recluse spider (genus Loxosceles) bites may lead to cutaneous and systemic manifestations known as loxoscelism. We report the second known case of spider bite poisoning in Malta caused by Loxosceles rufescens (Mediterranean recluse spider). A young adult female presented with localised erythema and pain on her left thigh after a witnessed spider bite. Over a few days, the area developed features of dermonecrosis together with systemic symptoms, including fever, fatigue and a generalised erythematous eruption...
July 20, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27424870/niche-modelling-of-the-chilean-recluse-spider-loxosceles-laeta-and-araneophagic-spitting-spider-scytodes-globula-and-risk-for-loxoscelism-in-chile
#15
M Canals, A Taucare-Rios, A D Brescovit, F Peña-Gomez, G Bizama, A Canals, L Moreno, R Bustamante
In Chile, all necrotic arachnidism is attributed to the Chilean recluse spider Loxosceles laeta (Nicolet) (Araneae: Sicariidae). It is predated by the spitting spider Scytodes globula (Nicolet) (Araneae: Scytodidae). The biology of each of these species is not well known and it is important to clarify their distributions. The aims of this study are to elucidate the variables involved in the niches of both species based on environmental and human footprint variables, and to construct geographic maps that will be useful in estimating potential distributions and in defining a map of estimated risk for loxoscelism in Chile...
July 18, 2016: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/27371689/estimation-of-the-effect-of-the-predator-scytodes-globula-araneae-scytodidae-on-loxosceles-laeta-araneae-sicariidae-populations
#16
Mauricio Canals, Lucila Moreno, Rigoberto Solís
It has been reported that Scytodes spiders are predators of spiders of the genus Loxosceles, but the question of the effect of Scytodes globula Nicolet on Loxosceles laeta (Nicolet) populations is still unanswered. The goal of this study is to analyze the population effect of S. globula on the population dynamics of L. laeta by means of simulation with projection matrices, considering the seasonal fluctuation of fecundity, random meetings between the predator and the prey, and limited growth of the L. laeta population...
August 2016: Environmental Entomology
https://www.readbyqxmd.com/read/27343628/expression-and-immunological-cross-reactivity-of-lalp3-a-novel-astacin-like-metalloprotease-from-brown-spider-loxosceles-intermedia-venom
#17
Adriano M Morgon, Matheus R Belisario-Ferrari, Dilza Trevisan-Silva, Gabriel O Meissner, Larissa Vuitika, Brenda Marin, Alexandre K Tashima, Luiza H Gremski, Waldemiro Gremski, Andrea Senff-Ribeiro, Silvio S Veiga, Olga M Chaim
Loxosceles spiders' venom comprises a complex mixture of biologically active toxins, mostly consisting of low molecular mass components (2-40 kDa). Amongst, isoforms of astacin-like metalloproteases were identified through transcriptome and proteome analyses. Only LALP1 (Loxosceles Astacin-Like protease 1) has been characterized. Herein, we characterized LALP3 as a novel recombinant astacin-like metalloprotease isoform from Loxosceles intermedia venom. LALP3 cDNA was cloned in pET-SUMO vector, and its soluble heterologous expression was performed using a SUMO tag added to LALP3 to achieve solubility in Escherichia coli SHuffle T7 Express LysY cells, which express the disulfide bond isomerase DsbC...
September 2016: Biochimie
https://www.readbyqxmd.com/read/27265457/protective-antibodies-against-a-sphingomyelinase-d-from-loxosceles-intermedia-spider-venom-elicited-in-mice-with-different-genetic-background
#18
Camila Franco Batista Oliveira, Andrea Vilela, Luis Augusto M Coura, Fernandes Tenório Gomes Rodrigues, Ronaldo Alves Pinto Nagem, Carlos Chávez-Olortegui, Tatiani U Maioli, Liza F Felicori
In the present investigation we used a recombinant LiD1 toxin, named rLiD1his, from Loxosceles intermedia brown spider to elicit specific antibodies in mice carrying different Human Leukocyte Antigens class II (HLAII) {DRB1.0401 (DR4), DQB1.0601 (DQ6) and DQB1.0302 (DQ8)} as well as in BALB/C and C57BL/6 control mice. All mice strains produced high antibody titers against rLiD1his but DR4 mice antibodies (the lower responder mice) were not able to recognize L. intermedia crude venom. The anti-rLiD1his sera, except from DR4 mice, were able to neutralize dermonecrotic, hemorrhagic and edematogenic effects of rLiD1his in naïve rabbits...
July 19, 2016: Vaccine
https://www.readbyqxmd.com/read/27259372/characterization-of-the-gene-encoding-component-c3-of-the-complement-system-from-the-spider-loxosceles-laeta-venom-glands-phylogenetic-implications
#19
D T Myamoto, G Pidde-Queiroz, A Pedroso, R M Gonçalves-de-Andrade, C W van den Berg, D V Tambourgi
A transcriptome analysis of the venom glands of the spider Loxosceles laeta, performed by our group, in a previous study (Fernandes-Pedrosa et al., 2008), revealed a transcript with a sequence similar to the human complement component C3. Here we present the analysis of this transcript. cDNA fragments encoding the C3 homologue (Lox-C3) were amplified from total RNA isolated from the venom glands of L. laeta by RACE-PCR. Lox-C3 is a 5178 bps cDNA sequence encoding a 190kDa protein, with a domain configuration similar to human C3...
September 2016: Immunobiology
https://www.readbyqxmd.com/read/27233517/active-site-mapping-of-loxosceles-phospholipases-d-biochemical-and-biological-features
#20
L Vuitika, D Chaves-Moreira, I Caruso, M A Lima, F H Matsubara, M T Murakami, H K Takahashi, M S Toledo, M A Coronado, H B Nader, A Senff-Ribeiro, O M Chaim, R K Arni, S S Veiga
Brown spider phospholipases D from Loxosceles venoms are among the most widely studied toxins since they induce dermonecrosis, triggering inflammatory responses, increase vascular permeability, cause hemolysis, and renal failure. The catalytic (H12 and H47) and metal-ion binding (E32 and D34) residues in Loxosceles intermedia phospholipase D (LiRecDT1) were mutated to understand their roles in the observed activities. All mutants were identified using whole venom serum antibodies and a specific antibody to wild-type LiRecDT1, they were also analyzed by circular dichroism (CD) and differential scanning calorimetry (DSC)...
September 2016: Biochimica et Biophysica Acta
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