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Di-cyclic gmp

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https://www.readbyqxmd.com/read/29146598/structural-analyses-unravel-the-molecular-mechanism-of-cyclic-di-gmp-regulation-of-bacterial-chemotaxis-via-a-pilz-adaptor-protein
#1
Xin-Fu Yan, Lingyi Xin, Jackie Tan Yen, Yukai Zeng, Shengyang Jin, Qing Wei Cheang, Rachel Andrea Chea Yuen Fong, Keng-Hwee Chiam, Zhao-Xun Liang, Yong-Gui Gao
The bacterial second messenger cyclic di-GMP (c-di-GMP) has emerged as a prominent mediator of bacterial physiology, motility and pathogenicity. Cdi-GMP often regulates the function of its protein targets through a unique mechanism that involves a discrete PilZ adaptor protein. However, the molecular mechanism in c-di-GMP-mediated protein regulation is unclear. Here, we present the structure of the PilZ adaptor protein MapZ co-crystallized in complex with c-di-GMP and its protein target CheR1, a chemotaxis-regulating methyltransferase in Pseudomonas aeruginosa This co-crystal structure, together with the structure of free CheR1, revealed that the binding of c-di-GMP induces dramatic structural changes in MapZ that are crucial for CheR1 binding...
November 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29091392/thiol-benzo-triazolo-quinazolinone-covalently-modifies-alg44-to-inhibit-c-di-gmp-binding-and-reduces-alginate-production-by-pseudomonas-aeruginosa
#2
Eric Zhou, Anna B Seminara, Soo-Kyoung Kim, Cherisse L Hall, Yan Wang, Vincent T Lee
Pseudomonas aeruginosa is an opportunistic pathogen that affects a large proportion of cystic fibrosis (CF) patients. CF patients have dehydrated mucus within the airways that leads to the inability of the mucociliary escalator to expel inhaled microbes. Once inhaled, P. aeruginosa can persist in the lungs of the CF patients for the remainder of their lives. During this chronic infection, a phenomenon called mucoid conversion can occur in which P. aeruginosa can mutate and inactivate their mucA gene. As a consequence, transcription of the alg operon is highly expressed leading to the copious secretion of the alginate exopolysaccharide, which is associated with decreased lung function and increased CF patient morbidity and mortality...
November 1, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/29091032/cyclic-di-gmp-differentially-tunes-a-bacterial-flagellar-motor-through-a-novel-class-of-chey-like-regulators
#3
Jutta Nesper, Isabelle Hug, Setsu Kato, Chee-Seng Hee, Judith Maria Habazettl, Pablo Manfredi, Stephan Grzesiek, Tilman Schirmer, Thierry Emonet, Urs Jenal
The flagellar motor is a sophisticated rotary machine facilitating locomotion and signal transduction. Owing to its important role in bacterial behavior, its assembly and activity are tightly regulated. For example, chemotaxis relies on a sensory pathway coupling chemical information to rotational bias of the motor through phosphorylation of the motor switch protein CheY. Using a chemical proteomics approach, we identified a novel family of CheY-like (Cle) proteins in Caulobacter crescentus, which tune flagellar activity in response to binding of the second messenger c-di-GMP to a C-terminal extension...
November 1, 2017: ELife
https://www.readbyqxmd.com/read/29090193/reduced-intracellular-c-di-gmp-content-increases-expression-of-quorum-sensing-regulated-genes-in-pseudomonas-aeruginosa
#4
Song Lin Chua, Yang Liu, Yingying Li, Hui Jun Ting, Gurjeet S Kohli, Zhao Cai, Pipob Suwanchaikasem, Kelvin Kau Kit Goh, Sean Pin Ng, Tim Tolker-Nielsen, Liang Yang, Michael Givskov
Cyclic-di-GMP (c-di-GMP) is an intracellular secondary messenger which controls the biofilm life cycle in many bacterial species. High intracellular c-di-GMP content enhances biofilm formation via the reduction of motility and production of biofilm matrix, while low c-di-GMP content in biofilm cells leads to increased motility and biofilm dispersal. While the effect of high c-di-GMP levels on bacterial lifestyles is well studied, the physiology of cells at low c-di-GMP levels remains unclear. Here, we showed that Pseudomonas aeruginosa cells with high and low intracellular c-di-GMP contents possessed distinct transcriptome profiles...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29072834/light-regulated-synthesis-of-cyclic-di-gmp-by-a-bidomain-construct-of-the-cyanobacteriochrome-tlr0924-sesa-without-stable-dimerization
#5
Matthew D Blain-Hartung, Nathan Clarke Rockwell, J Clark Lagarias
Phytochromes and cyanobacteriochromes (CBCRs) use double bond photoisomerization of their linear tetrapyrrole (bilin) chromophores within cGMP-specific phosphodiesterases/Adenylyl cyclases/FhlA (GAF) domain-containing photosensory modules to regulate signaling activity of C-terminal output domains. CBCRs exhibit much more diverse photocycles than phytochromes, and they are often found in large modular proteins such as Tlr0924 (SesA), one of three blue light regulators of cell aggregation in the cyanobacterium Thermosynechococcus elongatus...
October 26, 2017: Biochemistry
https://www.readbyqxmd.com/read/29068284/the-agrobacterium-tumefaciens-chey-like-protein-clar-regulates-biofilm-formation
#6
Nathan Feirer, DohHyun Kim, Jing Xu, Nico Fernandez, Christopher M Waters, Clay Fuqua
The switch from a motile, planktonic existence to an attached biofilm is a major bacterial lifestyle transition that is often mediated by complex regulatory pathways. In this report, we describe a CheY-like protein required for control of the motile-to-sessile switch in the plant pathogen Agrobacterium tumefaciens. This regulator, which we have designated ClaR, possesses two distinct CheY-like receiver (REC) domains and is involved in the negative regulation of biofilm formation, through production of the unipolar polysaccharide (UPP) adhesin and cellulose...
October 25, 2017: Microbiology
https://www.readbyqxmd.com/read/29063141/cmpx-affects-virulence-in-pseudomonas-aeruginosa-through-the-gac-rsm-signaling-pathway-and-by-modulating-c-di-gmp-levels
#7
Anjali Y Bhagirath, Deepti Somayajula, Yanqi Li, Kangmin Duan
Pseudomonas aeruginosa is an ubiquitous organism which is able to infect and colonize many types of hosts including humans. Colonization of P. aeruginosa in chronic infections leads to the formation of biofilms, which are difficult to eradicate. P. aeruginosa is capable of regulating its virulence factors in response to external environment triggers and its signaling mechanism involves two-component regulatory systems and small molecules such as bis-(3'-5')-cyclic dimeric guanosine monophosphate. PA1611-RetS-GacS/A-RsmA/Y/Z is a key regulatory pathway in P...
October 23, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29056046/synthesis-of-all-possible-canonical-3-5-linked-cyclic-dinucleotides-and-evaluation-of-riboswitch-interactions-and-immune-stimulatory-effects
#8
Changhao Wang, Malte Sinn, Julia Stifel, Anna C Heiler, Annette Sommershof, Jörg S Hartig
The cyclic dinucleotides (CDNs) c-di-GMP, c-di-AMP, and c-AMP-GMP are widely utilized as second messengers in bacteria, where they signal lifestyle changes such as motility and biofilm formation, cell wall and membrane homeostasis, virulence, and exo-electrogenesis. For all known bacterial CDNs, specific riboswitches have been identified that alter gene expression in response to the second messengers. In addition, bacterial CDNs trigger potent immune responses, making them attractive as adjuvants in immune therapies...
November 2, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29052983/in-vivo-biochemistry-single-cell-dynamics-of-cyclic-di-gmp-in-e-coli-in-response-to-zinc-overload
#9
Jongchan Yeo, Andrew B Dippel, Xin C Wang, Ming C Hammond
Intracellular signaling enzymes drive critical changes in cellular physiology and gene expression, but their endogenous activities in vivo remain highly challenging to study in real-time and for individual cells. Here we show that flow cytometry can be performed in complex media to monitor single-cell population distributions and dynamics of cyclic di-GMP signaling, which controls the bacterial colonization program. These in vivo biochemistry experiments are enabled by our second-generation RNA-based fluorescent (RBF) biosensors, which exhibit high fluorescence turn-on in response to cyclic di-GMP...
October 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/29038053/flagellar-motility-extracellular-proteases-and-vibrio-cholerae-detachment-from-abiotic-and-biotic-surfaces
#10
Loree Mewborn, Jorge A Benitez, Anisia J Silva
Vibrio cholerae of serogroups O1 and O139, the causative agent of Asiatic cholera, continues to be a major global health threat. This pathogen utilizes substratum-specific pili to attach to distinct surfaces in the aquatic environment and the human small intestine and detaches when conditions become unfavorable. Both attachment and detachment are critical to bacterial environmental survival, pathogenesis and disease transmission. However, the factors that promote detachment are less understood. In this study, we examine the role of flagellar motility and hemagglutinin/protease (HapA) in vibrio detachment from a non-degradable abiotic surface and from the suckling mouse intestine...
October 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29034850/pseudomonas-aeruginosa-variants-obtained-from-veterinary-clinical-samples-reveal-a-role-for-cyclic-di-gmp-in-biofilm-formation-and-colony-morphology
#11
Maria T Brock, Galya C Fedderly, Grace I Borlee, Michael M Russell, Liliana K Filipowska, Doreene R Hyatt, Ryan A Ferris, Bradley R Borlee
Overuse of antibiotics is contributing to an emerging antimicrobial resistance crisis. To better understand how bacteria adapt tolerance and resist antibiotic treatment, Pseudomonas aeruginosa isolates obtained from infection sites sampled from companion animals were collected and evaluated for phenotypic differences. Selected pairs of clonal isolates were obtained from individual infection samples and were assessed for antibiotic susceptibility, cyclic di-GMP levels, biofilm production, motility and genetic-relatedness...
October 16, 2017: Microbiology
https://www.readbyqxmd.com/read/29018125/more-than-enzymes-that-make-or-break-cyclic-di-gmp-local-signaling-in-the-interactome-of-ggdef-eal-domain-proteins-of-escherichia-coli
#12
Olga Sarenko, Gisela Klauck, Franziska M Wilke, Vanessa Pfiffer, Anja M Richter, Susanne Herbst, Volkhard Kaever, Regine Hengge
The bacterial second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) ubiquitously promotes bacterial biofilm formation. Intracellular pools of c-di-GMP seem to be dynamically negotiated by diguanylate cyclases (DGCs, with GGDEF domains) and specific phosphodiesterases (PDEs, with EAL or HD-GYP domains). Most bacterial species possess multiple DGCs and PDEs, often with surprisingly distinct and specific output functions. One explanation for such specificity is "local" c-di-GMP signaling, which is believed to involve direct interactions between specific DGC/PDE pairs and c-di-GMP-binding effector/target systems...
October 10, 2017: MBio
https://www.readbyqxmd.com/read/28990312/cyclic-di-gmp-regulation-of-virulence-in-bacterial-pathogens
#13
REVIEW
Cherisse L Hall, Vincent T Lee
Signaling pathways allow bacteria to adapt to changing environments. For pathogenic bacteria, signaling pathways allow for timely expression of virulence factors and the repression of antivirulence factors within the mammalian host. As the bacteria exit the mammalian host, signaling pathways enable the expression of factors promoting survival in the environment and/or nonmammalian hosts. One such signaling pathway uses the dinucleotide cyclic-di-GMP (c-di-GMP), and many bacterial genomes encode numerous proteins that are responsible for synthesizing and degrading c-di-GMP...
October 8, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28970274/a-model-of-equine-endometritis-involving-a-pseudomonas-aeruginosa-biofilm
#14
Ryan A Ferris, Patrick M McCue, Grace I Borlee, Kristina E Glapa, Kevin H Martin, Mihnea R Mangalea, Margo L Hennet, Lisa M Wolfe, Corey D Broeckling, Bradley R Borlee
Bacteria in a biofilm community have increased tolerance to antimicrobial therapy. To characterize the role of biofilms in equine endometritis, six mares were inoculated with lux-engineered Pseudomonas aeruginosa strains isolated from equine uterine infections. Following establishment of infection, the horses were euthanized and the endometrial surfaces were imaged for luminescence to localize adherent lux-labeled bacteria. Samples from the endometrium were collected for cytology, histopathology, carbohydrate analysis, and expression of inflammatory cytokine genes...
October 2, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28968917/fleq-regulates-both-the-type-vi-secretion-system-and-flagella-in-pseudomonas-putida
#15
Yuzhou Wang, Ye Li, Jianli Wang, Xiaoyuan Wang
Based on the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, flagellin FliC and a key member of the type VI secretion system Hcp1 were found to be secreted from Pseudomonas putida KT2442 cells in the late exponential phase, but in the early exponential phase only FliC was secreted. Relevant genes fleQ and rpoN were then individually deleted in P. putida KT2442, and the expression and transcription of fliC and hcp1 in these mutant strains were analyzed. In both △fleQ and △rpoN mutant cells, Hcp1 but not FliC was secreted in the late exponential phase, and it was secreted even in the early exponential phase...
October 2, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28956397/-regulation-of-c-di-gmp-metabolism-and-biofilm-formation-in-yersinia-pestis
#16
Juping Zhao, Yicheng Sun
Yersinia pestis, the cause of plague, is transmitted by flea bite. Y. pestis forms a biofilm in the proventriculus of its flea vector to enhance transmission. Biofilm formation in Y. pestis is positively regulated by the intracellular levels of the second messenger cyclic diguanylate (c-di-GMP). The c-di-GMP in Y. pestis is synthesized by two diguanylate cyclases (DGC), HmsT and HmsD, and degraded by phosphodiesterase (PDE), HmsP. Here we summarized the regulators that modulate c-di-GMP metabolism and biofilm formation in Y...
September 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28956394/-progress-in-c-di-gmp-inhibitors
#17
Xuwen Xiang, Xingyu Liu, Hui Tao, Zining Cui, Lianhui Zhang
The cyclic dinucleotide c-di-GMP is known as an important second messenger in bacteria, which controls various important cellular processes, such as cell differentiation, biofilm formation and virulence factors production. It is extremely vital for the development of new antibacterial agents by virtue of blocking c-di-GMP signal conduction. Current research indicates that there are three potential targets for discovering new antibacterial agents based on c-di-GMP regulated signal pathway, which are c-di-GMP synthases, c-di-GMP degrading enzymes and c-di-GMP receptors...
September 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28956389/-identification-of-cyclic-di-gmp-protein-receptors-high-throughput-screening-strategies-and-experimental-verification
#18
Shouting Cheng, Fangfang Wang, Wei Qian
cyclic di-GMP (c-di-GMP) is a universal second messenger in bacterial cells. It regulates various biological processes such as biofilm development, pathogenicity, motility, exopolysaccharide (EPS) production and cell cycle. The second messenger exerts its function by binding to effectors, such as riboswitches and proteins. However, due to the diverse conformations of c-di-GMP, its effectors are hardly to be predicted by homology search. Identification of c-di-GMP effectors is the initial step to investigate its regulatory function in bacterial signal transduction, however, it remains to be a technically difficult task...
September 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28956387/-structure-and-function-of-c-di-gmp-riboswitches
#19
Xinfeng Li, Fang Chen, Jinfeng Xiao, Jin He
Cyclic diguanosine monophosphate (c-di-GMP) is a ubiquitous nucleotide second messenger present in a wide variety of bacteria. It regulates many important bacterial physiological functions such as biofilm formation, motility, adhesion, virulence and extracellular polysaccharide synthesis. It binds with many different proteins or RNA receptors, one of which is called riboswitch that is usually located at the 5'-untranslational region (5'-UTR) in some mRNA. Riboswitch usually comprises a specific ligand-binding (sensor) domain (named aptamer domain, AD), as well as a variable domain, termed expression platform (EP), to regulate expression of downstream coding sequences...
September 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28956352/mutation-of-the-cyclic-di-gmp-phosphodiesterase-gene-in-burkholderia-lata-sk875-attenuates-virulence-and-enhances-biofilm-formation
#20
Hae-In Jung, Yun-Jung Kim, Yun-Jung Lee, Hee-Soo Lee, Jung-Kee Lee, Soo-Ki Kim
Burkholderia sp. is a gram-negative bacterium that commonly exists in the environment, and can cause diseases in plants, animals, and humans. Here, a transposon mutant library of a Burkholderia lata isolate from a pig with swine respiratory disease in Korea was screened for strains showing attenuated virulence in Caenorhabditis elegans. One such mutant was obtained, and the Tn5 insertion junction was mapped to rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that functions as a receptor. Mutation of rpfR caused a reduction in growth on CPG agar and swimming motility as well as a rough colony morphology on Congo red agar...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
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