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https://www.readbyqxmd.com/read/29064007/production-of-steviol-from-steviol-glucosides-using-%C3%AE-glucosidase-from-a-commercial-pectinase-sumizyme-px
#1
Thi Thanh Hanh Nguyen, Changseop Seo, Bon-Cheol Gu, Hee-Jung Lim, Jung-Min Ha, Seong-Bo Kim, Jun-Seong Park, Doman Kim
OBJECTIVE: To purify and characterize a specific enzyme from a commercial pectinase for the production of steviol from stevioside (Ste) without adding organic solvent and to improve steviol production. RESULTS: Commercial Sumizyme PX converted Ste to steviol with a yield of 98%. β-Glucosidase from Sumizyme PX (βglyPX) was purified in three steps with 12.5-fold purification and 51% yield. The specific activity of the purified βglyPX was 141 U/mg. The molecular weight of βglyPX was ~ 116 kDa on SDS-PAGE...
October 24, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29038773/a-fusion-protein-strategy-for-soluble-expression-of-stevia-glycosyltransferase-ugt76g1-in-escherichia-coli
#2
Liangliang Chen, Ping Sun, Yan Li, Ming Yan, Lin Xu, Kequan Chen, Pingkai Ouyang
The UDP-glucosyltransferase UGT76G1 from Stevia rebaudiana converts stevioside to rebaudioside A via a one-step glycosylation reaction, which increases the amount of sweet-tasting rebaudioside A and decreases the amount of stevioside that has a bitter aftertaste. This enzyme could, therefore, conceivably be used to improve the organoleptic properties of steviol glycosides and offer a cost-effective preparation of high-purity rebaudioside A. Producing soluble enzymes by overexpression is a prerequisite for large-scale biocatalysis...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/28991641/cardioprotection-of-stevioside-on-stunned-rat-hearts-a-mechano-energetical-study
#3
María I Ragone, Patricia Bonazzola, Germán A Colareda, María Lara Lazarte, Fiorella Bruno, Alicia E Consolini
BACKGROUND: The sweetener and hypoglycemic properties of stevioside (STV) are well known, as the main component of the plant Stevia rebaudiana. Given its extensive use in diabetic patients, it was of interest to evaluate its effects on the most frequent cardiovascular disease, the coronary insufficiency. PURPOSE: To study whether STV could be cardioprotective against ischemia-reperfusion (I/R) in a model of "stunning" in rat hearts. STUDY DESIGN: A preclinical study was performed in isolated hearts from rats in the following groups: non-treated rats whose hearts were perfused with STV 0...
November 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28958513/beaming-steviol-glycoside-analysis-into-the-next-dimension
#4
Benno F Zimmermann
Nine state-of-the-art reversed phase (RP) columns for ultra-high performance liquid chromatography were tested for the separation of steviol glycosides. The main criteria were resolution of the critical peak pair rebaudioside A and stevioside and the retention time of rebaudioside D. Three columns yielded a resolution of 2 or more of the critical peak pair and two of them showed sufficient retention of rebaudioside D, namely 1.62 and 1.84min corresponding to retention factors of 0.98 and 1.24. The separation of nine steviol glycosides was possible in 11min with UV and MS compatible, buffer-free eluents at moderate temperature...
February 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28947927/glycosides-from-stevia-rebaudiana-bertoni-possess-insulin-mimetic-and-antioxidant-activities-in-rat-cardiac-fibroblasts
#5
Cecilia Prata, Laura Zambonin, Benedetta Rizzo, Tullia Maraldi, Cristina Angeloni, Francesco Vieceli Dalla Sega, Diana Fiorentini, Silvana Hrelia
Stevia rebaudiana Bertoni is a shrub having a high content of sweet diterpenoid glycosides in its leaves, mainly stevioside and rebaudioside A, which are used as noncaloric, natural sweeteners. The aim of this study was to deepen the knowledge about the insulin-mimetic effect exerted by four different mixtures of steviol glycosides, rich in stevioside and rebaudioside A, in neonatal rat cardiac fibroblasts. The potential antioxidant activity of these steviol glycosides was also assessed, as oxidative stress is associated with diabetes...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28934139/phyllodulcin-a-natural-sweetener-regulates-obesity-related-metabolic-changes-and-fat-browning-related-genes-of-subcutaneous-white-adipose-tissue-in-high-fat-diet-induced-obese-mice
#6
Eunju Kim, Soo-Min Lim, Min-Soo Kim, Sang-Ho Yoo, Yuri Kim
Phyllodulcin is a natural sweetener found in Hydrangea macrophylla var. thunbergii. This study investigated whether phyllodulcin could improve metabolic abnormalities in high-fat diet (HFD)-induced obese mice. Animals were fed a 60% HFD for 6 weeks to induce obesity, followed by 7 weeks of supplementation with phyllodulcin (20 or 40 mg/kg body weight (b.w.)/day). Stevioside (40 mg/kg b.w./day) was used as a positive control. Phyllodulcin supplementation reduced subcutaneous fat mass, levels of plasma lipids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol and improved the levels of leptin, adiponectin, and fasting blood glucose...
September 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28922721/hydroalcoholic-extract-of-stevia-rebaudiana-bert-leaves-and-stevioside-ameliorates-lipopolysaccharide-induced-acute-liver-injury-in-rats
#7
Latha S, Sheetal Chaudhary, Ray R S
Oxidative stress and hepatic inflammatory response is primarily implicated in the pathogenesis of LPS induced acute liver injury. Stevioside, a diterpenoidal glycoside isolated from the Stevia rebaudiana leaves, exerts potent anti-oxidant, anti-inflammatory and immunomodulatory activities. The present study was aimed to investigate the hepatoprotective effect of hydroalcoholic extract of Stevia rebaudiana leaves (STE EXT) and its major phytochemical constituent, stevioside (STE) in LPS induced acute liver injury...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28903608/influence-of-substrates-on-the-in-vitro-kinetics-of-steviol-glucuronidation-and-interaction-between-steviol-glycosides-metabolites-and-ugt2b7
#8
Jun-Ming Chen, Yong-Mei Xia, Yan-Dong Zhang, Tong-Tong Zhang, Qing-Rui Peng, Yun Fang
Steviol glycosides, a natural sweetener, may perform bioactivities via steviol, their main metabolite in human digestion. The metabolising kinetics, i.e. glucuronidation kinetics and interaction between steviol glycosides or their metabolites and metabolising enzyme, are important for understanding the bioactivity and cytotoxicity. The present study investigated kinetics of steviol glucuronidation in human liver microsome and a recombinant human UDP-glucuronosyltransferases isomer, UGT2B7, along with molecular docking to analyse interaction between UGT2B7 and steviol or glucose...
September 13, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28887089/steviol-an-aglycone-of-steviol-glycoside-sweeteners-interacts-with-the-pregnane-x-pxr-and-aryl-hydrocarbon-ahr-receptors-in-detoxification-regulation
#9
Jan Dusek, Alejandro Carazo, Frantisek Trejtnar, Lucie Hyrsova, Ondřej Holas, Tomas Smutny, Stanislav Micuda, Petr Pavek
Stevia rebaudiana Bertoni is a herb known for the high content of natural sweeteners in its leaves. Its main secondary metabolite stevioside is used as non-caloric sweetener. No information, however, is available on whether stevioside or steviol interact with drug-metabolizing enzymes and pose the potential risk of food-drug interactions. Similarly, data are lacking on the interactions of steviol and stevioside with key nuclear receptors controlling the expression of the main drug metabolizing enzymes. We studied the interactions of steviol and stevioside with the pregnane X (PXR), vitamin D (VDR), constitutive androstane (CAR), farnesoid X (FXR), glucocorticoid (GR) and aryl hydrocarbon (AHR) receptors, which control expression of genes of xenobiotic metabolism...
September 5, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28886311/effect%C3%A2-of%C3%A2-sucrose-concentrations-on-stevia-rebaudiana-bertoni-tissue-culture-and-gene-expression
#10
T Ghorbani, D Kahrizi, M Saeidi, I Arji
Stevia rebaudiana (Bert.) Bertoni is known as sweet plant which it contains a high level of steviol glycosides in the leaves.  This plant has been used from centuries ago as a sweetener for tea. One of the most important steviol glycosides is stevioside that is attractive for diabetic persons. Tissue culture is the only rapid process for the mass propagation of stevia. One of the most important factors in the medium is sucrose that is a necessary for plant growth. In the present study, we use nodal segments of the stem as explants in mediums with different sucrose concentration (50 mM, 100mM and 150mM)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28876399/new-seminal-variety-of-stevia-rebaudiana-obtaining-fractions-with-high-antioxidant-potential-of-leaves
#11
Paula G Milani, Maysa Formigoni, Antonio S Dacome, Livia Benossi, Cecília E M DA Costa, Silvio C DA Costa
The aim of this study was to determine the composition and antioxidant potential of leaves of a new variety of Stevia rebaudiana (Stevia UEM-13). Stevia leaves of UEM-13 contain rebaudioside A as the main glycoside, while most wild Stevia plants contain stevioside. Furthermore can be multiplied by seed, which reduces the cost of plant culture techniques as other clonal varieties are multiplied by buds, requiring sophisticated and expensive seedling production systems. Ethanol and methanol were used in the extraction to determine the bioactive compounds...
July 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28838349/effect-of-kh2po4-on-gene-expression-morphological-and-biochemical-characteristics-of-stevia-rebaudiana-bertoni-under-in-vitro-conditions
#12
D Kahrizi, S M Ghari, M Ghaheri, F Fallah, T Ghorbani, A Beheshti Ale Agha, E Kazemi, Z Ansarypour
Stevia rebaudiana is one of the most important biologically sourced and low-calorie sweeteners Bertoni that has a lot of steviol glycosides. Tissue culture is the best for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. Therefore, the effect of different concentrations of KH2PO4on stevia growth factors and gene expression had been studied by tissue culture methods, RT-PCR and HPLC. According the results, bud numbers had increased significantly in MS + 0...
August 15, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28838348/effect-of-salinity-on-gene-expression-morphological-and-biochemical-characteristics-of-stevia-rebaudiana-bertoni-under-in-vitro-conditions
#13
F Fallah, F Nokhasi, M Ghaheri, D Kahrizi, A Beheshti Ale Agha, T Ghorbani, E Kazemi, Z Ansarypour
Stevia rebaudiana Bertoni is a famous medicinal plant for its low calorific value compounds which are named steviol glycosides (SGs) and they are 150-300 times sweeter than sugar. Among various SGs, stevioside and rebaudioside A considered to be the main sweetening compounds.  Soil salinity is one of the most essential stress in the world. Salinity affects the survival and yield of crops. In current study the effects of salinity and osmotic stress caused by different concentration of NaCl (0, 20, 40, 60 and 80 mM) on morphological traits, genes expressionand amount of both stevioside and rebaudioside Aunder in vitro conditions has been investigated...
August 15, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28763974/development-of-low-calorie-jams-with-increased-content-of-natural-dietary-fibre-made-from-tomato-pomace
#14
Miona Belović, Aleksandra Torbica, Ivana Pajić-Lijaković, Jasna Mastilović
In this study, four jam formulations were developed, starting with the basic formulation (Jam 1) containing sucrose and without added pectin. Sucrose was partially (50%) replaced by stevioside in formulations of Jam 2 and 3, while in Jam 4 sucrose was completely replaced by fructose and stevioside, making this formulation suitable for diabetic patients. Jam formulations 1 and 2, prepared without added pectin, were thermally stable in the temperature range of 25-90°C, which indicate their potential use as fruit fillings...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28754349/effect-of-steviol-steviol-glycosides-and-stevia-extract-on-glucocorticoid-receptor-signaling-in-normal-and-cancer-blood-cells
#15
Christina Panagiotou, Chrysovalantou Mihailidou, George Brauhli, Olga Katsarou, Paraskevi Moutsatsou
The use of steviol glycosides as non-caloric sweeteners has proven to be beneficial for patients with type 2 diabetes mellitus (T2D), obesity, and metabolic syndrome. However, recent data demonstrate that steviol and stevioside might act as glucocorticoid receptor (GR) agonists and thus correlate with adverse effects on metabolism. Herein, we evaluated the impact of steviol, steviol glycosides, and a Greek-derived stevia extract on a number of key steps of GR signaling cascade in peripheral blood mononuclear cells (PBMCs) and in Jurkat leukemia cells...
July 25, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28748072/the-evaluation-of-saccharose-replacing-by-adding-stevioside-maltodextrin-mixture-on-the-physicochemical-and-sensory-properties-of-naanberenji-an-iranian-confectionary
#16
Amirpouya Ghandehari Yazdi, Mohammad Hojjatoleslamy, Javad Keramat, Mahshid Jahadi, Elahe Amani
Stevia is a natural, non-nutritive sweetener can replace sugar in your diet to control diabetes and aid in weight loss. Naanberenji, an Iranian traditional cookie, is a well-reputed confectionary containing high sucrose and calorie value, for this reason its consumption in healthy diet has been restricted. In this study, the effect of sucrose replacement by stevioside-maltodextrin mixture on physicochemical properties of Naanberenji was evaluated in four replacing levels including 25%, 50%, 75%, and 100%. Results of texture evaluation revealed that hardness increases due to adding stevioside-maltodextrin (p < ...
July 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28716847/nonnutritive-sweeteners-and-cardiometabolic-health-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials-and-prospective-cohort-studies
#17
REVIEW
Meghan B Azad, Ahmed M Abou-Setta, Bhupendrasinh F Chauhan, Rasheda Rabbani, Justin Lys, Leslie Copstein, Amrinder Mann, Maya M Jeyaraman, Ashleigh E Reid, Michelle Fiander, Dylan S MacKay, Jon McGavock, Brandy Wicklow, Ryan Zarychanski
BACKGROUND: Nonnutritive sweeteners, such as aspartame, sucralose and stevioside, are widely consumed, yet their long-term health impact is uncertain. We synthesized evidence from prospective studies to determine whether routine consumption of non-nutritive sweeteners was associated with long-term adverse cardiometabolic effects. METHODS: We searched MEDLINE, Embase and Cochrane Library (inception to January 2016) for randomized controlled trials (RCTs) that evaluated interventions for nonnutritive sweeteners and prospective cohort studies that reported on consumption of non-nutritive sweeteners among adults and adolescents...
July 17, 2017: CMAJ: Canadian Medical Association Journal, Journal de L'Association Medicale Canadienne
https://www.readbyqxmd.com/read/28714761/gamma-irradiation-of-medicinally-important-plants-and-the-enhancement-of-secondary-metabolite-production
#18
P Vivek Vardhan, Lata I Shukla
PURPOSE: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secondary metabolites, their mode of synthesis and effect of γ-radiation on their yield for different plants, organs and in vitro cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying the increase in secondary metabolites...
September 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28709091/evaluating-the-photocatalytic-treatment-of-stevioside-by-tio2-in-different-aqueous-matrices-and-identification-of-transformation-products
#19
V A Sakkas, M Sarro, M Kalaboka, V Santoro, T Albanis, P Calza, C Medana
The present study reports the photocatalytic transformation of stevioside, under simulated solar irradiation using TiO2 as a photocatalyst. As a tool of investigating the effect of various aqueous matrices, as well as, the initial stevioside concentration on the variation of the photocatalytic efficiency, a fully nested experimental design was employed. A significant impact on the degradation rate of the sweetener was observed: degradation rate decreases in the order distilled water>river water>lake water, attributed to the increased natural organic matter content of the respective natural water samples...
December 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28666887/stevia-and-stevioside-protect-against-cisplatin-nephrotoxicity-through-inhibition-of-erk1-2-stat3-and-nf-%C3%AE%C2%BAb-activation
#20
Iva Potočnjak, Dalibor Broznić, Marija Kindl, Matija Kropek, Sanda Vladimir-Knežević, Robert Domitrović
We investigated the effect of natural sweetener Stevia rebaudiana and its constituent stevioside in cisplatin (CP)-induced kidney injury. Male BALB/cN mice were orally administered 10, 20, and 50 mg/kg body weight of Stevia rebaudiana ethanol extract (SE) or stevioside 50 mg/kg, 48 h after intraperitoneal administration of CP (13 mg/kg). Two days later, CP treatment resulted in histopathological changes showing kidney injury. Increased expression of 4-hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), and heme oxygenase-1 (HO-1) in mice kidneys suggested oxidative stress...
September 2017: Food and Chemical Toxicology
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