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Stevia rebaudiana

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https://www.readbyqxmd.com/read/29072099/stevia-rebaudiana-targeting-%C3%AE-amylase-an-in-vitro-and-in-silico-mechanistic-study
#1
Ramit Singla, Navdeep Singla, Vikas Jaitak
Diabetes mellitus (DM) is the fastest growing metabolic disorder in the world. Recently, more attention is paid to the study of natural products due to side effects of synthetic drugs. Stevia rebaudiana (Bertoni) is considered an encouraging starting point for the antidiabetic lead development. In the present study, the in vitro α-amylase inhibitory activity of the extracts of S. rebaudiana is investigated. In order to understand the molecular mechanism and future pharmacophore development, in silico study of secondary metabolites isolated from S...
October 26, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29063136/coupling-biological-detection-to-liquid-chromatography-a-new-tool-in-drug-discovery
#2
M A Campuzano-Bublitz, J G Hernández-Jiménez, R González-Brito, M S Montesinos, J J Fernández, J G Díaz, R Borges
Procedures to characterize drugs that can be obtained from plant extracts or combinatorial chemistry are tedious, and they consume considerable resources (e.g., animals) and time. Thus, we have looked for a way to streamline this process. We describe here a novel system for the pre-characterization of drugs based on liquid chromatography coupled to biological detection using perifused or perfused organs. This novel system allows the on-line detection of pharmacologically active substances in hydrosoluble mixtures from vegetal extracts or combinatorial chemistry libraries...
October 23, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29038773/a-fusion-protein-strategy-for-soluble-expression-of-stevia-glycosyltransferase-ugt76g1-in-escherichia-coli
#3
Liangliang Chen, Ping Sun, Yan Li, Ming Yan, Lin Xu, Kequan Chen, Pingkai Ouyang
The UDP-glucosyltransferase UGT76G1 from Stevia rebaudiana converts stevioside to rebaudioside A via a one-step glycosylation reaction, which increases the amount of sweet-tasting rebaudioside A and decreases the amount of stevioside that has a bitter aftertaste. This enzyme could, therefore, conceivably be used to improve the organoleptic properties of steviol glycosides and offer a cost-effective preparation of high-purity rebaudioside A. Producing soluble enzymes by overexpression is a prerequisite for large-scale biocatalysis...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/28991641/cardioprotection-of-stevioside-on-stunned-rat-hearts-a-mechano-energetical-study
#4
María I Ragone, Patricia Bonazzola, Germán A Colareda, María Lara Lazarte, Fiorella Bruno, Alicia E Consolini
BACKGROUND: The sweetener and hypoglycemic properties of stevioside (STV) are well known, as the main component of the plant Stevia rebaudiana. Given its extensive use in diabetic patients, it was of interest to evaluate its effects on the most frequent cardiovascular disease, the coronary insufficiency. PURPOSE: To study whether STV could be cardioprotective against ischemia-reperfusion (I/R) in a model of "stunning" in rat hearts. STUDY DESIGN: A preclinical study was performed in isolated hearts from rats in the following groups: non-treated rats whose hearts were perfused with STV 0...
November 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28958553/pretreatment-with-ethanol-as-an-alternative-to-improve-steviol-glycosides-extraction-and-purification-from-a-new-variety-of-stevia
#5
Maysa Formigoni, Paula Gimenez Milani, Alexandre da Silva Avíncola, Vanessa Jorge Dos Santos, Livia Benossi, Antônio Sergio Dacome, Eduardo Jorge Pilau, Silvio Claudio da Costa
Leaves of a new variety of Stevia rebaudiana with a high content of rebaudioside A were pretreated with ethanol. The ethanolic extract showed high antioxidant potential and 39 compounds were identified, by UPLC/HRMS, among them one not yet mentioned in the literature for stevia leaves. From the in natura leaves and pretreated leaves, the conditions of aqueous extraction of steviol glycosides were investigated using response surface methodology. The aqueous extracts obtained were purified by ion exchange chromatography techniques and membrane separation methods...
February 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28947927/glycosides-from-stevia-rebaudiana-bertoni-possess-insulin-mimetic-and-antioxidant-activities-in-rat-cardiac-fibroblasts
#6
Cecilia Prata, Laura Zambonin, Benedetta Rizzo, Tullia Maraldi, Cristina Angeloni, Francesco Vieceli Dalla Sega, Diana Fiorentini, Silvana Hrelia
Stevia rebaudiana Bertoni is a shrub having a high content of sweet diterpenoid glycosides in its leaves, mainly stevioside and rebaudioside A, which are used as noncaloric, natural sweeteners. The aim of this study was to deepen the knowledge about the insulin-mimetic effect exerted by four different mixtures of steviol glycosides, rich in stevioside and rebaudioside A, in neonatal rat cardiac fibroblasts. The potential antioxidant activity of these steviol glycosides was also assessed, as oxidative stress is associated with diabetes...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28928460/molecular-dissection-of-transcriptional-reprogramming-of-steviol-glycosides-synthesis-in-leaf-tissue-during-developmental-phase-transitions-in-stevia-rebaudiana-bert
#7
Gopal Singh, Gagandeep Singh, Pradeep Singh, Rajni Parmar, Navgeet Paul, Radhika Vashist, Mohit Kumar Swarnkar, Ashok Kumar, Sanatsujat Singh, Anil Kumar Singh, Sanjay Kumar, Ram Kumar Sharma
Stevia is a natural source of commercially important steviol glycosides (SGs), which share biosynthesis route with gibberellic acids (GAs) through plastidal MEP and cytosolic MVA pathways. Ontogeny-dependent deviation in SGs biosynthesis is one of the key factor for global cultivation of Stevia, has not been studied at transcriptional level. To dissect underlying molecular mechanism, we followed a global transcriptome sequencing approach and generated more than 100 million reads. Annotation of 41,262 de novo assembled transcripts identified all the genes required for SGs and GAs biosynthesis...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28922721/hydroalcoholic-extract-of-stevia-rebaudiana-bert-leaves-and-stevioside-ameliorates-lipopolysaccharide-induced-acute-liver-injury-in-rats
#8
Latha S, Sheetal Chaudhary, Ray R S
Oxidative stress and hepatic inflammatory response is primarily implicated in the pathogenesis of LPS induced acute liver injury. Stevioside, a diterpenoidal glycoside isolated from the Stevia rebaudiana leaves, exerts potent anti-oxidant, anti-inflammatory and immunomodulatory activities. The present study was aimed to investigate the hepatoprotective effect of hydroalcoholic extract of Stevia rebaudiana leaves (STE EXT) and its major phytochemical constituent, stevioside (STE) in LPS induced acute liver injury...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28887089/steviol-an-aglycone-of-steviol-glycoside-sweeteners-interacts-with-the-pregnane-x-pxr-and-aryl-hydrocarbon-ahr-receptors-in-detoxification-regulation
#9
Jan Dusek, Alejandro Carazo, Frantisek Trejtnar, Lucie Hyrsova, Ondřej Holas, Tomas Smutny, Stanislav Micuda, Petr Pavek
Stevia rebaudiana Bertoni is a herb known for the high content of natural sweeteners in its leaves. Its main secondary metabolite stevioside is used as non-caloric sweetener. No information, however, is available on whether stevioside or steviol interact with drug-metabolizing enzymes and pose the potential risk of food-drug interactions. Similarly, data are lacking on the interactions of steviol and stevioside with key nuclear receptors controlling the expression of the main drug metabolizing enzymes. We studied the interactions of steviol and stevioside with the pregnane X (PXR), vitamin D (VDR), constitutive androstane (CAR), farnesoid X (FXR), glucocorticoid (GR) and aryl hydrocarbon (AHR) receptors, which control expression of genes of xenobiotic metabolism...
September 5, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28886311/effect%C3%A2-of%C3%A2-sucrose-concentrations-on-stevia-rebaudiana-bertoni-tissue-culture-and-gene-expression
#10
T Ghorbani, D Kahrizi, M Saeidi, I Arji
Stevia rebaudiana (Bert.) Bertoni is known as sweet plant which it contains a high level of steviol glycosides in the leaves.  This plant has been used from centuries ago as a sweetener for tea. One of the most important steviol glycosides is stevioside that is attractive for diabetic persons. Tissue culture is the only rapid process for the mass propagation of stevia. One of the most important factors in the medium is sucrose that is a necessary for plant growth. In the present study, we use nodal segments of the stem as explants in mediums with different sucrose concentration (50 mM, 100mM and 150mM)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28876399/new-seminal-variety-of-stevia-rebaudiana-obtaining-fractions-with-high-antioxidant-potential-of-leaves
#11
Paula G Milani, Maysa Formigoni, Antonio S Dacome, Livia Benossi, Cecília E M DA Costa, Silvio C DA Costa
The aim of this study was to determine the composition and antioxidant potential of leaves of a new variety of Stevia rebaudiana (Stevia UEM-13). Stevia leaves of UEM-13 contain rebaudioside A as the main glycoside, while most wild Stevia plants contain stevioside. Furthermore can be multiplied by seed, which reduces the cost of plant culture techniques as other clonal varieties are multiplied by buds, requiring sophisticated and expensive seedling production systems. Ethanol and methanol were used in the extraction to determine the bioactive compounds...
July 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28838349/effect-of-kh2po4-on-gene-expression-morphological-and-biochemical-characteristics-of-stevia-rebaudiana-bertoni-under-in-vitro-conditions
#12
D Kahrizi, S M Ghari, M Ghaheri, F Fallah, T Ghorbani, A Beheshti Ale Agha, E Kazemi, Z Ansarypour
Stevia rebaudiana is one of the most important biologically sourced and low-calorie sweeteners Bertoni that has a lot of steviol glycosides. Tissue culture is the best for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. Therefore, the effect of different concentrations of KH2PO4on stevia growth factors and gene expression had been studied by tissue culture methods, RT-PCR and HPLC. According the results, bud numbers had increased significantly in MS + 0...
August 15, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28838348/effect-of-salinity-on-gene-expression-morphological-and-biochemical-characteristics-of-stevia-rebaudiana-bertoni-under-in-vitro-conditions
#13
F Fallah, F Nokhasi, M Ghaheri, D Kahrizi, A Beheshti Ale Agha, T Ghorbani, E Kazemi, Z Ansarypour
Stevia rebaudiana Bertoni is a famous medicinal plant for its low calorific value compounds which are named steviol glycosides (SGs) and they are 150-300 times sweeter than sugar. Among various SGs, stevioside and rebaudioside A considered to be the main sweetening compounds.  Soil salinity is one of the most essential stress in the world. Salinity affects the survival and yield of crops. In current study the effects of salinity and osmotic stress caused by different concentration of NaCl (0, 20, 40, 60 and 80 mM) on morphological traits, genes expressionand amount of both stevioside and rebaudioside Aunder in vitro conditions has been investigated...
August 15, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28792778/stevia-rebaudiana-bertoni-a-natural-alternative-for-treating-diseases-associated-with-metabolic-syndrome
#14
Areli Carrera-Lanestosa, Yolanda Moguel-Ordóñez, Maira Segura-Campos
Stevia rebaudiana (SR) is often used by the food industry due to its steviol glycoside content, which is a suitable calorie-free sweetener. Further, both in vitro and in vivo studies indicate that these glycosides and the extracts from SR have pharmacological and therapeutic properties, including antioxidant, antimicrobial, antihypertensive, antidiabetic, and anticancer. This work reviews the antiobesity, antihyperglycemic, antihypertensive, and antihyperlipidemic effects of the majority of glycosides and aqueous/alcoholic extracts from the leaves, flowers, and roots of the SR...
October 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28762654/development-of-a-high-performance-liquid-chromatography-procedure-to-identify-known-and-detect-novel-c-13-oligosaccharide-moieties-in-diterpene-glycosides-from-stevia-rebaudiana-bertoni-bertoni-asteraceae-structure-elucidation-of-rebaudiosides-v-and-w
#15
Wilmer H Perera, Ion Ghiviriga, Douglas L Rodenburg, Raquel Carvalho, Kamilla Alves, James D McChesney
As an aid for structure elucidation of new steviol glycosides, reversed-phase C18 high-performance liquid chromatography method was developed with several previously characterized diterpene glycosides, to identify known and detect novel aglycone-C13 oligosaccharide moieties and indirectly identify C-19 interlinkages. Elution order of several diterpene glycosides and their aglycone-C13 oligosaccharide substituted with different sugar arrangements were also summarized. Comparison of the retention time of a product obtained after alkaline hydrolysis with the aglycone-C-13 portions of known compounds reported herein allowed us to deduce the exact positions of the sugars in the C-13 oligosaccharide portion...
August 1, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28758125/antihyperglycemic-and-antihyperlipidemic-activity-of-hydroponic-stevia-rebaudiana-aqueous-extract-in-hyperglycemia-induced-by-immobilization-stress-in-rabbits
#16
Anush Aghajanyan, Zaruhi Movsisyan, Armen Trchounian
Diabetes mellitus (DM) is a serious worldwide problem related to human hyperglycemia. Thus, herbal preparations with antihyperglycemic properties especially leaf extracts of hydroponic Stevia rebaudiana (SR) would be useful in hyperglycemia treatment. The antihyperglycemic potential of this medicinal plant grown using hydroponics methods has been evaluated. Significant reduction of some biochemical characteristics for sugars and fatty acids in blood, liver, and muscle especially fasting glucose levels, serum triglycerides, LDL-cholesterol, total cholesterol levels, and increased HDL-cholesterol ones was shown with SR aqueous extract treatment...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28742480/from-ca%C3%A3-eh%C3%A3-to-a-commercial-sweetener-the-diterpenoid-glycosides-of-stevia-rebaudiana
#17
James R Hanson
The isolation, structure elucidation, chemistry, biosynthesis and biological activity of the sweet steviol glycosides from Stevia rebaudiana, are reviewed.
December 1, 2016: Science Progress
https://www.readbyqxmd.com/read/28738695/a-research-on-the-genotoxicity-of-stevia-in-human-lymphocytes
#18
Aslı Uçar, Serkan Yılmaz, Şemsigül Yılmaz, Mustafa Sefa Kılıç
Stevia extracts are obtained from Stevia rebaudiana commonly used as natural sweeteners. It is ∼250-300 times sweeter than sucrose. Common use of stevia prompted us to investigate its genotoxicity in human peripheral blood lymphocytes. Stevia (active ingredient steviol glycoside) was dissolved in pure water. Dose selection was done using ADI (acceptable daily intake) value. Negative control (pure water), 1, 2, 4, 8 and 16 μg/ml concentrations which were equivalent to ADI/4, ADI/2, ADI, ADI × 2 and ADI × 4 of Stevia were added to whole-blood culture...
July 24, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28720959/fortification-of-the-whey-protein-isolate-antioxidant-and-antidiabetic-activity-with-fraction-rich-in-phenolic-compounds-obtained-from-stevia-rebaudiana-bert-bertoni-leaves
#19
Paula Gimenez Milani, Maysa Formigoni, Yago Carvalho Lima, Silvano Piovan, Giuliana Maria Ledesma Peixoto, Daiane Montoia Camparsi, Willian do Nascimento da Silva Rodrigues, Jordana Quaglia Pereira da Silva, Alexandre da Silva Avincola, Eduardo Jorge Pilau, Cecília Edna Mareze da Costa, Silvio Cláudio da Costa
A stevia fraction (ASF) free of steviol glycosides was extracted from Stevia rebaudiana leaves (Stevia UEM-13). ASF essentially constitutes phenolic compounds (52.42%), which were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) as caffeic acid, quercetin-3-o-glycoside, cyanidin-3-glucoside, kaempferol, quercetin, apigenin, rozmarinic acid, chlorogenic acid and dicaffeoylquinic acid. ASF was used as a multi-functional source of phenolic compounds to fortify the whey protein isolate (WPI) obtained by membrane separation...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28714761/gamma-irradiation-of-medicinally-important-plants-and-the-enhancement-of-secondary-metabolite-production
#20
P Vivek Vardhan, Lata I Shukla
PURPOSE: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secondary metabolites, their mode of synthesis and effect of γ-radiation on their yield for different plants, organs and in vitro cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying the increase in secondary metabolites...
September 2017: International Journal of Radiation Biology
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