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Stevia rebaudiana

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https://www.readbyqxmd.com/read/28372164/optimization-of-microwave-assisted-extraction-of-total-extract-stevioside-and-rebaudioside-a-from-stevia-rebaudiana-bertoni-leaves-using-response-surface-methodology-rsm-and-artificial-neural-network-ann-modelling
#1
Kashif Ameer, Seong-Woo Bae, Yunhee Jo, Hyun-Gyu Lee, Asif Ameer, Joong-Ho Kwon
Stevia rebaudiana (Bertoni) consists of stevioside and rebaudioside-A (Reb-A). We compared response surface methodology (RSM) and artificial neural network (ANN) modelling for their estimation and predictive capabilities in building effective models with maximum responses. A 5-level 3-factor central composite design was used to optimize microwave-assisted extraction (MAE) to obtain maximum yield of target responses as a function of extraction time (X1: 1-5min), ethanol concentration, (X2: 0-100%) and microwave power (X3: 40-200W)...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28364791/effect-of-nitrogen-sources-on-some-morphological-characteristics-of-in-vitro-stevia-rebaudiana-bertoni
#2
F Akbari, A Arminian, D Kahrizi, A Fazeli
Stevia rebaudiana Bertoni belongs to Asteraceae family that leaves 200-300 times sweeter than sugar. Low seed fertility is one of the most important problems in Stevia production. So, Plant tissue culture is an efficient method for mass propagation of Stevia. In this research, we studied the effect of various concentrations of nitrogen on some morphological traits of stevia under in vitro conditions. We used axillary nodes as explants and they were cultured on Murashige and Skoog (MS) medium containing inorganic nitrogen sources i...
February 28, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28363166/insight-into-anti-diabetic-effect-of-low-dose-of-stevioside
#3
Vladimirka Ilić, Saša Vukmirović, Nebojša Stilinović, Ivan Čapo, Milan Arsenović, Boris Milijašević
Diabetes mellitus is a chronic disease characterized by abnormal carbohydrate, lipid and protein metabolism due to a lack of insulin or reduced target cell sensitivity to insulin. Stevia rebaudiana is an important source of biochemically active substances with proven anti-diabetic effect. The aim of this study was to determine anti-diabetic effects of the low dose of stevioside in NMRI Haan mice. Aqueous stevioside solution (20mg/kg body weight) was administered by oral route of administration. Anti-diabetic effect of stevioside was estimated by oral glucose tolerance test, adrenaline test after a 10day stevioside treatment, and alloxan induced hyperglycaemia in mice (two experimental groups, 10day stevioside treatment before and after alloxan administration)...
March 28, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28361903/steviol-glycosides-enhance-pancreatic-beta-cell-function-and-taste-sensation-by-potentiation-of-trpm5-channel-activity
#4
Koenraad Philippaert, Andy Pironet, Margot Mesuere, William Sones, Laura Vermeiren, Sara Kerselaers, Sílvia Pinto, Andrei Segal, Nancy Antoine, Conny Gysemans, Jos Laureys, Katleen Lemaire, Patrick Gilon, Eva Cuypers, Jan Tytgat, Chantal Mathieu, Frans Schuit, Patrik Rorsman, Karel Talavera, Thomas Voets, Rudi Vennekens
Steviol glycosides (SGs), such as stevioside and rebaudioside A, are natural, non-caloric sweet-tasting organic molecules, present in extracts of the scrub plant Stevia rebaudiana, which are widely used as sweeteners in consumer foods and beverages. TRPM5 is a Ca(2+)-activated cation channel expressed in type II taste receptor cells and pancreatic β-cells. Here we show that stevioside, rebaudioside A and their aglycon steviol potentiate the activity of TRPM5. We find that SGs potentiate perception of bitter, sweet and umami taste, and enhance glucose-induced insulin secretion in a Trpm5-dependent manner...
March 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/28351677/toxicological-evaluation-of-ethanolic-extract-from-stevia-rebaudiana-bertoni-leaves-genotoxicity-and-subchronic-oral-toxicity
#5
Qiannan Zhang, Hui Yang, Yongning Li, Haibo Liu, Xudong Jia
Stevia rebaudiana Bertoni leaves have a long history of use as an abundant source of sweetener. The aqueous extract of stevia leaves and the predominant constitutes steviol glycosides have been intensively investigated. However, rare studies provided toxicological evaluation of bioactive components in the polar extract regarding their safety on human health. This study aimed to evaluate the toxicity of ethanolic extract of Stevia rebaudiana Bertoni leaves through a battery of in vitro and in vivo tests. Negative results were unanimously obtained from bacterial reverse mutation assay, mouse bone marrow micronucleus assay and mouse sperm malformation assay...
March 25, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28346877/stevia-rebaudiana-bertoni-effect-on-the-hemolytic-potential-of-listeria-monocytogenes
#6
S Sansano, A Rivas, M C Pina-Pérez, A Martinez, D Rodrigo
The effect of Stevia rebaudiana Bertoni on the hemolytic potential of Listeria monocytogenes was studied by means of the assessment of the Listeriolysin O (LLO) production. The three factors under study, stevia concentration in the range [0-2.5] % (w/v), incubation temperature (10 and 37°C), and exposure time (0-65h) significantly affected (p≤0.05) the hemolytic activity of L. monocytogenes. Results showed that at the lower incubation temperature the hemolytic potential of the bacterium was significantly reduced, from 100% at 37°C to 8% at 10°C (after 65h of incubation) in unsupplemented substrate (0% stevia)...
March 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28343388/development-and-validation-of-an-enzymatic-method-to-determine-stevioside-content-from-stevia-rebaudiana
#7
Somsiri Udompaisarn, Dumrongkiet Arthan, Jamorn Somana
An enzymatic method for specific determination of stevioside content was established. Recombinant β-glucosidase BT_3567 (rBT_3567) from Bacteroides thetaiotaomicron HB-13 exhibited selective hydrolysis of stevioside at β-1,2-glycosidic bond to yield rubusoside and glucose. Coupling of this enzyme with glucose oxidase and peroxidase allowed for quantitation of stevioside content in Stevia samples by using a colorimetric-based approach. The series of reactions for stevioside determination can be completed within 1 h at 37 °C...
April 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28278673/development-of-an-efficient-high-performance-thin-layer-chromatography-method-for-determination-of-jasmonic-acid-in-leaf-tissue-of-stevia-rebaudiana-bertoni-bertoni
#8
Divya Kilam, M Saifi, A Agnihotri, M Z Abdin
Determination of endogenous levels of jasmonic acid (JA) is essential, as it plays a pivotal role in the physiological processes during a plant's life cycle. A high performance thin layer chromatography (HPTLC) method was developed for the detection and quantification of JA in leaf extracts of medicinal plant, Stevia rebaudiana (Bertoni) Bertoni. The separation was achieved using the solvents ethyl acetate-benzene (1:1, v/v) as the mobile phase, followed by scanning and quantification at 295 nm. Densitometric analysis of leaf extract resulted in compact spots for JA at Rf = 0...
February 13, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28251728/tandem-mass-spectrometry-a-convenient-approach-in-the-dosage-of-steviol-glycosides-in-stevia-sweetened-commercial-food-beverages
#9
Leonardo Di Donna, Fabio Mazzotti, Ilaria Santoro, Giovanni Sindona
The use of sweeteners extracted from leaves of the plant species Stevia Rebaudiana is worldwide increasing. They are recognized as GRAS (Generally Recognized as Safe) by US-FDA and approved by EU-EFSA, with some recommendation on the daily dosage that should not interfere with glucose metabolism. The results here presented introduce an easy analytical approach for the identification and assay of Stevia sweeteners in commercially available soft drink, based on Liquid Chromatography (LC) coupled to Tandem Mass Spectrometry (MS/MS), using a natural statin like molecule, Brutieridin, as internal standard...
March 1, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28218969/stability-of-the-stevia-derived-sweetener-rebaudioside-a-in-solution-as-affected-by-ultraviolet-light-exposure
#10
Jiewen Zhang, Leonard N Bell
Rebaudioside A is a natural noncaloric high-potency sweetener extracted from the leaves of Stevia rebaudiana. With rebaudioside A use increasing in foods, understanding the factors affecting its stability is necessary. This project evaluated the degradation rate constants of rebaudioside A in water, 0.1 M phosphate buffer, and 0.1 M citrate buffer at pH 3 and 7 as a function of ultraviolet (UV) light intensity (365 nm, 0 μW/cm(2) for dark conditions, 27 μW/cm(2) for low intensity, and 190 μW/cm(2) for high intensity) at 32...
April 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28215607/impact-of-blue-red-and-far-red-light-treatments-on-gene-expression-and-steviol-glycoside-accumulation-in-stevia-rebaudiana
#11
Yuki Yoneda, Hiroshi Nakashima, Juro Miyasaka, Katsuaki Ohdoi, Hiroshi Shimizu
Stevia rebaudiana (Bertoni) Bertoni is a plant that biosynthesizes a group of natural sweeteners that are up to approximately 400 times sweeter than sucrose. The sweetening components of S. rebaudiana are steviol glycosides (SGs) that partially share their biosynthesis pathway with gibberellins (GAs). However, the molecular mechanisms through which SGs levels can be improved have not been studied. Therefore, transcription levels of several SG biosynthesis-related genes were analyzed under several light treatments involved in GA biosynthesis...
May 2017: Phytochemistry
https://www.readbyqxmd.com/read/28193409/a-new-natural-source-for-obtainment-of-inulin-and-fructo-oligosaccharides-from-industrial-waste-of-stevia-rebaudiana-bertoni
#12
Sheila Mara Sanches Lopes, Gabriela Krausová, José Walter Pedroza Carneiro, José Eduardo Gonçalves, Regina Aparecida Correia Gonçalves, Arildo José Braz de Oliveira
Fructan-type inulin and fructo-oligosaccharides (FOS) are reserve polysaccharides that offer an interesting combination of nutritional and technological properties for food industry. Stevia rebaudiana is used commercially in the sweetener industry due to the high content of steviol glycosides in its leaves. With the proposal of using industrial waste, the objective of the present study was to isolate, characterize and evaluate the prebiotic activity of inulin and FOS from S. rebaudiana stems. The chemical characterization of the samples by GC-MS, NMR and off-line ESI-MS showed that it was possible to obtain inulin molecules from the S...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28146121/a-novel-diterpene-glycoside-with-nine-glucose-units-from-stevia-rebaudiana-bertoni
#13
Indra Prakash, Gil Ma, Cynthia Bunders, Romila D Charan, Catherine Ramirez, Krishna P Devkota, Tara M Snyder
Following our interest in new diterpene glycosides with better taste profiles than that of Rebaudioside M, we have recently isolated and characterized Rebaudioside IX-a novel steviol glycoside-from a commercially-supplied extract of Stevia rebaudiana Bertoni. This molecule contains a hexasaccharide group attached at C-13 of the central diterpene core, and contains three additional glucose units when compared with Rebaudioside M. Here we report the complete structure elucidation-based on extensive Nuclear Magnetic Resonance (NMR) analysis (1H, 13C, Correlation Spectroscopy (COSY), Heteronuclear Single Quantum Coherence-Distortionless Enhancement Polarization Transfer (HSQC-DEPT), Heteronuclear Multiple Bond Correlation (HMBC), 1D Total Correlation Spectroscopy (TOCSY), Nuclear Overhauser Effect Spectroscopy (NOESY)) and mass spectral data-of this novel diterpene glycoside with nine sugar moieties and containing a relatively rare 16 α-linked glycoside...
January 31, 2017: Biomolecules
https://www.readbyqxmd.com/read/28108883/rapid-solid-liquid-dynamic-extraction-rslde-a-new-rapid-and-greener-method-for-extracting-two-steviol-glycosides-stevioside-and-rebaudioside-a-from-stevia-leaves
#14
Monica Gallo, Manuela Vitulano, Anna Andolfi, Marina DellaGreca, Esterina Conte, Martina Ciaravolo, Daniele Naviglio
Stevioside and rebaudioside A are the main diterpene glycosides present in the leaves of the Stevia rebaudiana plant, which is used in the production of foods and low-calorie beverages. The difficulties associated with their extraction and purification are currently a problem for the food processing industries. The objective of this study was to develop an effective and economically viable method to obtain a high-quality product while trying to overcome the disadvantages derived from the conventional transformation processes...
January 20, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/27979591/rebaudiosides-t-and-u-minor-c-19-xylopyranosyl-and-arabinopyranosyl-steviol-glycoside-derivatives-from-stevia-rebaudiana-bertoni-bertoni
#15
Wilmer H Perera, Ion Ghiviriga, Douglas L Rodenburg, Kamilla Alves, John J Bowling, Bharathi Avula, Ikhlas A Khan, James D McChesney
Two diterpene glycosides were isolated from a commercial Stevia rebaudiana leaf extract. One was found to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid-(2-O-β-d-xylopyranosyl-3-O-β-d-glucopyranosyl- β-d-glucopyranosyl) ester (rebaudioside T), whereas the other was determined to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid-(6-O-α-l-arabinopyranosyl-β-d-glucopyranosyl) ester (rebaudioside U)...
March 2017: Phytochemistry
https://www.readbyqxmd.com/read/27979170/bioaccessibility-of-bioactive-compounds-after-non-thermal-processing-of-an-exotic-fruit-juice-blend-sweetened-with-stevia-rebaudiana
#16
COMPARATIVE STUDY
Magdalena Buniowska, Juana M Carbonell-Capella, Ana Frigola, Maria J Esteve
A comparative study of the bioaccessibility of bioactive compounds and antioxidant capacity in a fruit juice-Stevia rebaudiana mixture processed by pulsed electric fields (PEF), high voltage electrical discharges (HVED) and ultrasound (USN) technology at two equivalent energy inputs (32-256kJ/kg) was made using an in vitro model. Ascorbic acid was not detected following intestinal digestion, while HVED, PEF and USN treatments increased total carotenoid bioaccessibility. HVED at an energy input of 32kJ/kg improved bioaccessibility of phenolic compounds (34...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27923373/microbial-production-of-next-generation-stevia-sweeteners
#17
Kim Olsson, Simon Carlsen, Angelika Semmler, Ernesto Simón, Michael Dalgaard Mikkelsen, Birger Lindberg Møller
BACKGROUND: The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively, and have low sweetness thresholds, high maximum sweet intensities and exhibit a greatly reduced lingering bitter taste compared to stevioside and rebaudioside A, the most abundant steviol glucosides in the leaves of Stevia rebaudiana...
December 7, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27816105/stevia-glycosides-chemical-and-enzymatic-modifications-of-their-carbohydrate-moieties-to-improve-the-sweet-tasting-quality
#18
Gerrit J Gerwig, Evelien M Te Poele, Lubbert Dijkhuizen, Johannis P Kamerling
Stevia glycosides, extracted from the leaves of the plant Stevia rebaudiana Bertoni, display an amazing high degree of sweetness. As processed plant products, they are considered as excellent bio-alternatives for sucrose and artificial sweeteners. Being noncaloric and having beneficial properties for human health, they are the subject of an increasing number of studies for applications in food and pharmacy. However, one of the main obstacles for the successful commercialization of Stevia sweeteners, especially in food, is their slight bitter aftertaste and astringency...
2016: Advances in Carbohydrate Chemistry and Biochemistry
https://www.readbyqxmd.com/read/27813105/the-influence-of-the-rebaudioside-a-content-of-stevia-stevia-rebaudiana-bertoni-on-the-determination-of-sweetness-equivalence-in-bittersweet-chocolates-using-the-time-intensity-analysis
#19
Bruna M Azevedo, Janaína M M Ferreira, Valdecir Luccas, Helena M A Bolini
The consumption of diet products has increased greatly in recent years. The objectives of the study were to develop a bittersweet chocolate added inulin and stevias with different rebaudioside A contents (60%, 80%, and 97%). Five chocolate samples were formulated with different sucrose concentrations to determine the ideal sucrose concentration for bittersweet chocolate. The use of just-about-right scale identified an ideal sucrose concentration of 47.5% (w/w). The sweetness equivalence in sugar-free bittersweet chocolates was determined by the time-intensity method by 14 selected and trained judges...
December 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27798332/the-noncaloric-sweetener-rebaudioside-a-stimulates-glucagon-like-peptide-1-release-and-increases-enteroendocrine-cell-numbers-in-2-dimensional-mouse-organoids-derived-from-different-locations-of-the-intestine
#20
Nikkie van der Wielen, Jean Paul Ten Klooster, Susanne Muckenschnabl, Raymond Pieters, Henk Fj Hendriks, Renger F Witkamp, Jocelijn Meijerink
BACKGROUND: Glucagon-like peptide 1 (GLP-1) contributes to satiety and plays a pivotal role in insulin secretion and glucose homeostasis. Similar to GLP-1, peptide YY (PYY) and cholecystokinin also influence food intake. The secretion of these hormones by enteroendocrine cells along the intestine is modulated by nutrients. Preparations from the Stevia rebaudiana plant, including rebaudioside A, are increasingly being used as noncaloric sweeteners. OBJECTIVE: We investigated the effects of rebaudioside A on enteroendocrine cells by assessing both cell numbers as well as their secretory capacity in an organoid model...
December 2016: Journal of Nutrition
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