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Stevia rebaudiana

M Molina-Calle, F Priego-Capote, M D Luque de Castro
Stevia is currently a well-known plant thanks to its sweeting power. Numerous studies that elucidate its composition were exclusively focused on determination of steviol and its glycosides. Untargeted analysis was applied to obtain a profile of main compounds present in extracts from Stevia (Stevia rebaudiana Bertoni) leaves using LC-MS in high resolution mode with a quadrupole-time of flight analyzer. Eighty-nine compounds were tentatively identified and classified into different families: flavonoids; quinic and caffeic acids and derivatives; diterpenoids (including steviol and glycosides); sesquiterpenoids; amino acids and derivatives; fatty amides and derivatives; fatty acids and derivatives; oligosaccharides; glycerolipids; purines; and retinoids...
March 15, 2017: Food Chemistry
Václav Pavlíček, Petr Tůma
Two electrophoretic methods with contactless conductivity detection have been developed for determination of the content of rebaudioside A and stevioside in samples of sweeteners and beverages prepared from extracts of the plant Stevia rebaudiana Bertoni. The total content of rebaudioside A and stevioside can be determined in a fused silica capillary with an inner diameter of 10μm and total length of 31.5cm in optimised background electrolyte with the composition 170mM H3BO3/LiOH (pH 9.0). The combined peak of the two glucosides is characterised by a migration time of 54s, which completely separates it from EOF...
March 15, 2017: Food Chemistry
Thi Thanh Hanh Nguyen, Seong-Bo Kim, Nahyun M Kim, Choongil Kang, Byoungsang Chung, Jun-Seong Park, Doman Kim
Steviol is a diterpene isolated from the plant Stevia rebaudiana that has a potential role as an antihyperglycemic agent by stimulating insulin secretion from pancreatic beta cells and also has significant potential to diminish the renal clearance of anionic drugs and their metabolites. In this study, the lacS gene, which encodes a thermostable β-glycosidase (SSbgly) enzyme from the extremely thermoacidophillic archaeon Sulfolobus solfataricus, was cloned and expressed in E. coli Rossetta BL21(DE3)pLyS using lactose as an inducer...
November 2016: Enzyme and Microbial Technology
Luisa Torri, Alessandra Frati, Paolino Ninfali, Stefano Mantegna, Giancarlo Cravotto, Gabriella Morini
BACKGROUND: The demand for zero and reduced-sugar food products containing cocoa is expanding continuously. The study has been designed to evaluate the feasibility of producing high-quality chocolate sweetened with a crude extract of Stevia rebaudiana (Bertoni) prepared by a green microwave-assisted water-steam extraction procedure. Seven approximately isosweet chocolate formulations were developed mixing cocoa paste, sucrose, commercial stevioside, crude green extract and maltitol in different proportions...
September 20, 2016: Journal of the Science of Food and Agriculture
Shokoofeh Hajihashemi, Jan M C Geuns
Stevia rebaudiana is a sweet herb of the Astraceae family, which is cultivated for the natural sweeteners it contains. The aim of this study was to assess the effect of drought, simulated by the application of polyethylene glycol (5%, 10%, and 15% w/v), on the content of steviol glycosides (SVglys) and transcription levels of six genes involved in the biosynthesis of these natural sweeteners. The transcription levels of ent-kaurene synthase, ent-kaurene oxidase, ent-kaurenoic acid hydroxylase, and three UDP-dependent glycosyltransferases, UGT85C2,UGT74G1 and UGT76G1 were downregulated under polyethylene glycol treatment...
September 2016: FEBS Open Bio
Waldo Acevedo, Fernando González-Nilo, Eduardo Agosin
Stevia is one of the sweeteners with the greatest consumer demand because of its natural origin and minimal calorie content. Steviol glycosides (SG) are the main active compounds present in the leaves of Stevia rebaudiana and are responsible for its sweetness. However, recent in vitro studies in HEK 293 cells revealed that SG specifically activate the hT2R4 and hT2R14 bitter taste receptors, triggering this mouth feel. The objective of this study was to characterize the interaction of SG with these two receptors at the molecular level...
October 12, 2016: Journal of Agricultural and Food Chemistry
Yigit Uyanikgil, Turker Cavusoglu, Huseyin A Balcıoglu, Serkan Gurgul, Volkan Solmaz, Hatice K Ozlece, Nilgun Erten, Oytun Erbas
The safety of patients with epilepsy consuming sweetening agents, which is becoming increasingly prevalent for various reasons, is a topic that should be emphasized as sensitively as it is for other diseases. Patients with epilepsy consume sweetening agents for different reasons such being diabetic or overweight. They can occasionally be exposed to sweetening agents unrestrainedly through consuming convenience food, primarily beverages. This study aimed to investigate the effects of rebaudioside A (Reb-A), which is a steviol glycoside produced from the herb Stevia rebaudiana (Bertoni), on epileptic seizures and convulsions induced by pentylenetetrazole (PTZ)...
September 2016: Kaohsiung Journal of Medical Sciences
Nabilatul Hani Mohd-Radzman, Wan Iryani Wan Ismail, Siti Safura Jaapar, Zainah Adam, Aishah Adam
[This corrects the article DOI: 10.1155/2013/938081.].
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Sheila Mara Sanches Lopes, Mariane Grigio Francisco, Bruna Higashi, Rafaela Takako Ribeiro de Almeida, Gabriela Krausová, Eduardo Jorge Pilau, José Eduardo Gonçalves, Regina Aparecida Correia Gonçalves, Arildo José Braz de Oliveira
Stevia rebaudiana (Bertoni) is widely studied because of its foliar steviol glycosides. Fructan-type polysaccharides were recently isolated from its roots. Fructans are reserve carbohydrates that have important positive health effects and technological applications in the food industry. The objective of the present study was to isolate and characterize fructo-oligosaccharides (FOSs) from S. rebaudiana roots and in vitro adventitious root cultures and evaluate the potential prebiotic effect of these molecules...
November 5, 2016: Carbohydrate Polymers
Irma Aranda-González, Yolanda Moguel-Ordóñez, Luis Chel-Guerrero, Maira Segura-Campos, David Betancur-Ancona
Steviol glycosides are a family of compounds found in Stevia rebaudiana Bertoni that are responsible for sweetness capacity. The antihyperglycemic effect of the two major steviol glycosides, Rebaudioside A and Stevioside, has been studied and it has been found that despite having the same common structure, only Stevioside exerts an antihyperglycemic effect. Although other steviol derivatives are found in smaller amounts (minor steviol glycosides) in S. rebaudiana, whether or not they possess antihyperglycemic activity has not been evaluated...
September 2016: Journal of Medicinal Food
Stefan Wolfrum, Julien Marcus, Didier Touraud, Werner Kunz
Soaps are the oldest and perhaps most natural surfactants. However, they lost much of their importance since "technical surfactants", usually based on sulfates or sulfonates, have been developed over the last fifty years. Indeed, soaps are pH- and salt-sensitive and they are irritant, especially to the eyes. In food emulsions, although authorized, they have a bad taste, and long-chain saturated soaps have a high Krafft temperature. We believe that most or perhaps all of these problems can be solved with modern formulation approaches...
October 2016: Advances in Colloid and Interface Science
Mohamed A Ibrahim, Douglas L Rodenburg, Kamilla Alves, Wilmer H Perera, Frank R Fronczek, John Bowling, James D McChesney
No abstract text is available yet for this article.
August 26, 2016: Journal of Natural Products
Perumal Venkatachalam, Thiyagarajan Manickam, Keuk-Soo Bang, Palanivel Velmurugan, Byung-Taek Oh
The present study was carried out to examine the antidiabetic effects of chitosan nanoparticles (CNPs) loaded with (Stevia rebaudiana leaf extract-SRLE) bioactive molecules in a rat model of streptozotocin (STZ) induced diabetes mellitus. Successful crosslinking of the bioactive molecules to the chitosan nanoparticles was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). The colloidal characteristics of the synthesized nanoparticles were revealed by X-ray Diffraction (XRD) analysis. Morphological analysis by Transmission Electron Microscopy (TEM) revealed that thebioactive molecule-loaded CNPs were well-dispersed and spherical or polygonal in shape with an average size of<73...
July 2, 2016: International Journal of Biological Macromolecules
Griet Dewitte, Maarten Walmagh, Margo Diricks, Alexander Lepak, Alexander Gutmann, Bernd Nidetzky, Tom Desmet
UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr)...
September 10, 2016: Journal of Biotechnology
Q Z Wang, M Huang, S R Downie, Z X Chen
Invasive plants tend to spread aggressively in new habitats and an understanding of their genetic diversity and population structure is useful for their management. In this study, expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed for the invasive plant species Praxelis clematidea (Asteraceae) from 5548 Stevia rebaudiana (Asteraceae) expressed sequence tags (ESTs). A total of 133 microsatellite-containing ESTs (2.4%) were identified, of which 56 (42.1%) were hexanucleotide repeat motifs and 50 (37...
2016: Genetics and Molecular Research: GMR
Rabia Javed, Muhammad Usman, Buhara Yücesan, Muhammad Zia, Ekrem Gürel
This study aims to address the effects of different concentrations (0, 0.1, 1.0, 10, 100 or 1000 mg L(-1)) of engineered zinc oxide (ZnO) nanoparticles (34 nm in size) on growth parameters, steviol glycosides (rebaudioside A and stevioside) production and antioxidant activities in the tissue culture grown shoots of Stevia rebaudiana Bertoni. The highest percentage of shoot formation (89.6%) at 1 mg L(-1) of ZnO nanoparticles concentration suggests a positive influence of ZnO nanoparticles on S. rebaudiana growth as compared to other treatments with or without ZnO nanoparticles...
May 24, 2016: Plant Physiology and Biochemistry: PPB
Raheleh Assaei, Pooneh Mokarram, Sanaz Dastghaib, Sara Darbandi, Mahsa Darbandi, Fatemeh Zal, Masoumeh Akmali, Gholam Hossein Ranjbar Omrani
BACKGROUND: Traditional medicines with anti-diabetic effects are considered suitable supplements to treat diabetes. Among medicinal herbs, Stevia Rebaudiana Bertoni is famous for its sweet taste and beneficial effect in regulation of glucose. However, little is known about the exact mechanism of stevia in pancreatic tissue. Therefore, this study investigated the possible effects of stevia on pancreas in managing hyperglycemia seen in streptozotocin-induced Sprague-Dawley rats. METHODS: Sprague-Dawley rats were divided into four groups including normoglycemic, diabetic and two more diabetic groups in which, one was treated with aquatic extract of stevia (400 mg/kg) and the other with pioglitazone (10 mg/kg) for the period of 28 days...
April 2016: Avicenna Journal of Medical Biotechnology
Mohamed A Ibrahim, Douglas L Rodenburg, Kamilla Alves, Wilmer H Perera, Frank R Fronczek, John Bowling, James D McChesney
Two new diterpene glycosides have been isolated from a commercial extract of the leaves of Stevia rebaudiana. Compound 1 was shown to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-xylopyranosyl)oxy]ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (rebaudioside R), while compound 2 was determined to be 13-[(2-O-α-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid 2-O-α-l-rhamnopyranosyl-β-d-glucopyranosyl ester (rebaudioside S). Six additional known compounds were identified, dulcoside B, 13-[(2-O-β-d-xylopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester, eugenol diglucoside, rebaudioside G, 13-[(2-O-6-deoxy-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester, and rebaudioside D (3), respectively...
May 27, 2016: Journal of Natural Products
Arpan Modi, Nitish Kumar, Subhash Narayanan
Stevia (Stevia rebaudiana Bertoni) is a medicinal plant having sweet, diterpenoid glycosides known as steviol glycosides which are 200-300 times sweeter than sucrose (0.4 % solution). They are synthesized mainly in the leaves via plastid localized 2-C-methyl-D-erythrose-4-phosphate pathway (MEP pathway). Fifteen genes are involved in the formation of these glycosides. In the present protocol, a method for the quantification of transcripts of these genes is shown. The work involves RNA extraction and cDNA preparation, and therefore, procedures for the confirmation of DNA-free cDNA preparation have also been illustrated...
2016: Methods in Molecular Biology
Arpan R Modi, Vikas Sharma, Ghanshyam Patil, Amritpal S Singh, N Subhash, Nitish Kumar
Here we describe an efficient micropropagation protocol for Stevia rebaudiana Bertoni. We present experiments carried out to optimize the suitable media for in vitro shoot multiplication and root induction and to study the effect of culture vessel on shoot multiplication. Among all different media tested for in vitro shoot multiplication, hormone-free liquid medium is most suitable. The highest number of nodes per shoot (5.4) and length of shoot (4.76 cm) at 4 weeks after subculturing are observed when single node explants are placed on modified MS medium supplemented with 1 % sucrose and 0...
2016: Methods in Molecular Biology
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