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miR-200b

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https://www.readbyqxmd.com/read/29779470/ascites-derived-extracellular-micrornas-as-potential-biomarkers-for-ovarian-cancer
#1
Luděk Záveský, Eva Jandáková, Vít Weinberger, Luboš Minář, Veronika Hanzíková, Daniela Dušková, Lenka Záveská Drábková, Iveta Svobodová, Aleš Hořínek
Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29778524/posttranscriptional-regulation-of-interleukin-33-expression-by-microrna-200-in-bronchial-asthma
#2
Xin Tang, Feng Wu, Jinshuo Fan, Yang Jin, Jianjun Wang, Guanghai Yang
The importance of understanding how interleukin-33 (IL-33) is regulated (particularly by miRs) is critical in IL-33 biology, and evidence of this in asthma pathology is limited. MicroRNA profiling of cells isolated from bronchoalveolar lavage of 14 asthmatic patients and 11 healthy controls revealed miR-200b and miR-200c were significantly reduced in asthmatic patients compared with healthy controls. The reduction was validated in two independent models of allergen-induced allergic airway inflammation and further demonstrated to be inversely correlated with asthma severity, as well as increased IL-33 production in asthmatic patients...
April 25, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29769539/integrated-analysis-of-hepatic-mrna-and-mirna-profiles-identified-molecular-networks-and-potential-biomarkers-of-nafld
#3
Mingzhe Zhu, Qianlei Wang, Wenjun Zhou, Tao Liu, Lili Yang, Peiyong Zheng, Li Zhang, Guang Ji
To enhance our understanding of molecular mechanisms and mine novel biomarkers of non-alcoholic fatty liver disease (NAFLD), RNA sequencing was performed to gain hepatic expression profiles of mRNAs and miRNAs in NAFLD and normal rats. Using DESeq with thresholds of a two-fold change and a false discovery rate (FDR) less than 0.05, 336 mRNAs and 21 miRNAs were identified as differentially expressed. Among those, 17 miRNAs (e.g., miR-144-3p, miR-99a-3p, miR-200b-3p, miR-200b-5p, miR-200c-3p, etc.) might serve as novel biomarkers of NAFLD...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29743493/the-kindlin-2-regulation-of-epithelial-to-mesenchymal-transition-in-breast-cancer-metastasis-is-mediated-through-mir-200b
#4
Khalid Sossey-Alaoui, Elzbieta Pluskota, Dorota Szpak, William P Schiemann, Edward F Plow
Metastasis is the main cause of death in cancer patients, including breast cancer (BC). Despite recent progress in understanding the biological and molecular determinants of BC metastasis, effective therapeutic treatments are yet to be developed. Among the multitude of molecular mechanisms that regulate cancer metastasis, the epithelial-to-mesenchymal transition (EMT) program plays a key role in the activation of the biological steps leading to the metastatic phenotype. Kindlin-2 has been associated with the pathogenesis of several types of cancers, including BC...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29738771/mir-141-3p-is-upregulated-in-esophageal-squamous-cell-carcinoma-and-targets-pleckstrin-homology-domain-leucine-rich-repeat-protein-phosphatase-2-a-negative-regulator-of-the-pi3k-akt-pathway
#5
Osamu Ishibashi, Ichiro Akagi, Yota Ogawa, Takashi Inui
The phosphatidylinositol-3-kinase (PI3K)/AKT pathway is frequently activated in various human cancers and plays essential roles in their development and progression. Accumulating evidence suggests that dysregulated expression of microRNAs (miRNAs) is closely associated with cancer progression and metastasis. Here, we focused on miRNAs that could regulate genes related to the PI3K/AKT pathway in esophageal squamous cell carcinoma (ESCC). To identify upregulated miRNAs and their possible target genes in ESCC, we performed microarray-based integrative analyses of miRNA and mRNA expression levels in three human ESCC cell lines and a normal esophageal epithelial cell line...
May 11, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29738694/downregulation-of-linc00894-002-contributes-to-tamoxifen-resistance-by-enhancing-the-tgf-%C3%AE-signaling-pathway
#6
Xiulei Zhang, Meiting Wang, Huihui Sun, Tao Zhu, Xiangting Wang
Tamoxifen is a widely used personalized medicine for estrogen receptor (ER)-positive breast cancer, but approximately 30% of patients receiving the treatment relapse due to tamoxifen resistance (TamR). Recently, several reports have linked lncRNAs to cancer drug resistance. However, the role of lncRNAs in TamR is unclear. To identify TamR-related lncRNAs, we first used a bioinformatic approach to predict whether they have connection with known TamR-associated genes by starBase v2.0 and divided them into two groups...
May 2018: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29738527/defensive-effect-of-microrna-200b-c-against-amyloid-beta-peptide-induced-toxicity-in-alzheimer-s-disease-models
#7
Sayuri Higaki, Masashi Muramatsu, Akio Matsuda, Kenji Matsumoto, Jun-Ichi Satoh, Makoto Michikawa, Shumpei Niida
MiRNA molecules are important post-transcriptional regulators of gene expression in the brain function. Altered miRNA profiles could represent a defensive response against the pathogenesis of neurodegenerative disorders, such as Alzheimer's disease (AD). Endogenous miRNAs have lower toxic effects than other gene silencing methods, thus enhancing the expression of defensive miRNA could be an effective therapy. However, little is known about the potential of targeting miRNAs for the treatment of AD. Here, we examined the function of the miR-200 family (miR-200a, -141, -429, -200b, -200c), identified using miRNA microarray analysis of cortical tissue from Tg2576 transgenic mice...
2018: PloS One
https://www.readbyqxmd.com/read/29737439/enos-expression-and-no-release-during-hypoxia-is-inhibited-by-mir-200b-in-human-endothelial-cells
#8
Anna Janaszak-Jasiecka, Anna Siekierzycka, Sylwia Bartoszewska, Marcin Serocki, Lawrence W Dobrucki, James F Collawn, Leszek Kalinowski, Rafal Bartoszewski
The nitric oxide (NO) secreted by vascular endothelium is required for the maintenance of cardiovascular homeostasis. Diminished release of NO generated by endothelial NO synthase contributes to endothelial dysfunction. Hypoxia and ischemia reduce endothelial eNOS expression via posttranscriptional mechanisms that result in NOS3 transcript destabilization. Here, we examine whether microRNAs contribute to this mechanism. We followed the kinetics of hypoxia-induced changes in NOS3 mRNA and eNOS protein levels in primary human umbilical vein endothelial cells (HUVECs)...
May 8, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29727505/integrated-microrna-and-mrna-sequencing-analysis-of-age-related-changes-to-mouse-thymic-epithelial-cells
#9
Hong-Ling Jia, Xiao-Qin Zeng, Feng Huang, Ya-Meng Liu, Bi-Shuang Gong, Kai-Zhao Zhang, Jiang-Hui Zeng, Dong-Guang Guo, Zhuo-Ya Wang, Yu-Gu Li
MicroRNAs (miRNAs) play key roles in the regulation of gene expression during multiple physiological processes, including early development, differentiation, and ageing. However, their involvement in age-related thymic involution is not clear. In this study, we profiled the global transcriptome and miRNAome of thymic epithelial cells in 1- and 3-month-old male and female mice, and predicted the possible transcription factors and target genes of the four most significantly differentially expressed miRNAs (DEMs) (miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p) by performing bioinformatics analyses...
May 4, 2018: IUBMB Life
https://www.readbyqxmd.com/read/29721089/high-discrimination-factor-nanosensor-based-on-tetrahedral-dna-nanostructures-and-gold-nanoparticles-for-detection-of-mirna-21-in-live-cells
#10
Shulian Bai, Bangtian Xu, Yongcan Guo, Juhui Qiu, Wen Yu, Guoming Xie
While detection of microRNA with or without signal amplification is highly informative, nanosensors with high specificity for cell-specific RNA detection are rare. Methods: In this study, a tetrahedral DNA nanostructure (TDN) with a specific function was combined with gold nanoparticles (Au-NP) possessing fluorescence quenching effects and a large surface area to fabricate a fluorescence resonance energy transfer based nanosensor (Au-TDNN). The presence of miR-21 (target) can separate the fluorescent dye-labeled detection probe on Au-TDNNs from Au-NPs, which separates the donor and acceptor, thus inducing an intensive fluorescence signal...
2018: Theranostics
https://www.readbyqxmd.com/read/29720943/microrna-200a-200b-modulate-high-glucose-induced-endothelial-inflammation-by-targeting-o-linked-n-acetylglucosamine-transferase-expression
#11
Wan-Yu Lo, Wen-Kai Yang, Ching-Tien Peng, Wan-Yu Pai, Huang-Joe Wang
Background and Aims: Increased O -linked N -acetylglucosamine ( O -GlcNAc) modification of proteins by O -GlcNAc transferase (OGT) is associated with diabetic complications. Furthermore, oxidative stress promotes endothelial inflammation during diabetes. A previous study reported that microRNA-200 (miR-200) family members are sensitive to oxidative stress. In this study, we examined whether miR-200a and miR-200b regulate high-glucose (HG)-induced OGT expression in human aortic endothelial cells (HAECs) and whether miRNA-200a/200b downregulate OGT expression to control HG-induced endothelial inflammation...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29702103/the-mir-200b-200a-429-cluster-prevents-metastasis-and-induces-dormancy-in-a-murine-claudin-low-mammary-tumor-cell-line
#12
Katrina L Watson, Robert A Jones, Bruce Anthony, Roger A Moorehead
The miR-200 family of microRNAs consisting of miR-141, miR-200a, miR-200b, miR-200c and miR-429 are emerging as important regulators of breast cancer progression. This family of microRNAs maintain mammary epithelial identity and downregulation of miR-200 expression has been associated with epithelial-to-mesenchymal transition in mammary tumors. Therefore, re-expression of one or more miR-200 family members in mammary tumor cells with mesenchymal characteristics may restore an epithelial phenotype including growth and metastasis suppression...
April 24, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29689389/deep-sequencing-of-small-rnas-in-blood-of-patients-with-brain-arteriovenous-malformations
#13
Yong Chen, Zhili Li, Yi Shi, Guangfu Huang, Longyi Chen, Haibin Tan, Zhenyu Wang, Cheng Yin, Junting Hu
BACKGROUND: The deregulation of circulating microRNAs (miRNAs) is always associated with development and progression of human diseases. We are aimed to assess whether brain arteriovenous malformations (BAVMs) patients present a distinct miRNA signature compared with healthy subjects. METHODS: Three unruptured BAVMs patients and three normal controls were recruited as case and normal groups. Peripheral blood was collected and miRNA signature was obtained by next-generation sequencing, followed by comparative, functional, and network analyses...
April 21, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29588175/identification-and-analysis-of-micrornas-mrnas-pairs-associated-with-nutritional-status-in-seasonal-sheep
#14
Heng Yang, Xianxia Liu, Guangdong Hu, Yifan Xie, Shan Lin, Zongsheng Zhao, Jingbo Chen
Given the important role of nutritional status for reproductive performance, we aimed to explore the potential microRNA (miRNA)-mRNA pairs and their regulatory roles associated with nutritional status in seasonal reproducing sheep. Individual ewes were treated with and without 0.3 kg/day concentrates, and the body condition score, estrus rate, and related miRNAs and target genes were compared. A total of 261 differentially expressed miRNAs were identified, including 148 hypothalamus-expressed miRNAs and 113 ovary-expressed miRNAs, and 349 target genes were predicted to be associated with nutritional status and seasonal reproduction in sheep...
March 24, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29579206/a-mir-200-cluster-on-chromosome-23-regulates-sperm-motility-in-zebrafish
#15
Shuting Xiong, Wenge Ma, Jing Jing, Jin Zhang, Cheng Dan, Jian-Fang Gui, Jie Mei
Besides its well-documented roles in cell proliferation, apoptosis and carcinogenesis, the function of p53-microRNA axis has been recently revealed in reproductive system. Recent studies indicated that miR-200 family members are dys-regulated in nonobstructive azoospermia patients, whereas their functions remain poorly documented. The aims of this study were to investigate the function of miR-200 family on zebrafish testis development and sperm activity. There was no significant difference in testis morphology and histology between wildtype and knockout zebrafish with deletion of miR-200 cluster on chromosome 6 (chr6-miR-200-KO) or on chromosome 23 (chr23-miR-200-KO)...
March 19, 2018: Endocrinology
https://www.readbyqxmd.com/read/29577903/hypertonic-saline-regulates-microglial-m2-polarization-via-mir-200b-klf4-in-cerebral-edema-treatment
#16
Miaoyun Wen, Jingkun Ye, Yongli Han, Linqiang Huang, Hui Yang, Wenqiang Jiang, Shenglong Chen, Wenhong Zhong, Hongke Zeng, Daniel Y Li
BACKGROUND: Hypertonic saline (HS) has been used clinically for treatment of cerebral edema for decades. Previously we have demonstrated that HS alleviates cerebral edema via regulating water/ion channel protein and attenuating neuroinflammation. However, whether HS treatment triggers microglia polarization and its regulatory mechanism during this process is unclear. METHODS AND RESULTS: The Sprague-Dawley (SD) rats that underwent right-sided middle cerebral artery occlusion (MCAO) were used for assessment of neuroinflammation and microglia functions...
March 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29559342/microrna-signatures-in-diagnosis-and-prognosis-of-cutaneous-t-cell-lymphoma
#17
Xiaoyan Shen, Bo Wang, Kejia Li, Lili Wang, Xiaoqing Zhao, Feng Xue, Ruofei Shi, Jie Zheng
Cutaneous T cell lymphoma (CTCL) can have clinical and histological features resembling benign inflammatory dermatosis (BID) and can be difficult to diagnose. Very limited biomarkers are available for CTCL prognosis. We aimed to identify microRNA (miR) signatures to facilitate diagnostic and prognostic evaluations of CTCL. A cross-platform miR microarray identified 10 miRs that were differentially expressed between CTCL and BID patients. Subsequent RT-PCR validation was used to generate a 5-miR based diagnosing classifier, which showed high diagnostic accuracy in CTCL (AUC=0...
March 17, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29539618/non-proximal-renal-tubule-derived-urinary-exosomal-mir-200b-as-a-biomarker-of-renal-fibrosis
#18
Yanting Yu, Feng Bai, Nan Qin, Wenjin Liu, Qi Sun, Yang Zhou, Junwei Yang
BACKGROUND: Renal fibrosis is a common outcome of nearly all kinds of chronic kidney disease (CKD) and eventually progresses to end-stage renal disease. The identification of an optimal biomarker of renal fibrosis to replace the invasive renal biopsy will have important clinical implications. METHODS: We isolated urinary exosomes from 50 participants and examined the exosomal protein content and particle number in 38 CKD patients with different degrees of renal fibrosis and in 12 normal individuals...
March 14, 2018: Nephron
https://www.readbyqxmd.com/read/29534583/cisplatin-binds-to-pre-mir-200b-and-impairs-its-processing-to-mature-microrna
#19
R Mezencev, R M Wartell
Cisplatin is an important anticancer drug with a complex mode of action, a variety of possible targets, and numerous resistance mechanisms. While genomic DNA has traditionally been considered to be its most critical anticancer target, several lines of evidence suggest that various RNAs and other biomolecules may play a role in its anticancer mode of action. In this report we demonstrate that cisplatin modifies pre-miR-200b, impairs its processing to mature miRNA, and decreases miR-200b expression in ovarian cancer cells...
2018: Neoplasma
https://www.readbyqxmd.com/read/29416756/a-mirna-based-classification-of-renal-cell-carcinoma-subtypes-by-pcr-and-in-situ-hybridization
#20
Ashley Di Meo, Rola Saleeb, Samantha J Wala, Heba W Khella, Qiang Ding, Haiyan Zhai, Kalra Krishan, Adriana Krizova, Manal Gabril, Andrew Evans, Fadi Brimo, Maria D Pasic, Antonio Finelli, Eleftherios P Diamandis, George M Yousef
Renal cell carcinoma (RCC) constitutes an array of morphologically and genetically distinct tumors the most prevalent of which are clear cell, papillary, and chromophobe RCC. Accurate distinction between the typically benign-behaving renal oncocytoma and RCC subtypes is a frequent challenge for pathologists. This is critical for clinical decision making. Subtypes also have different survival outcomes and responses to therapy. We extracted RNA from ninety formalin-fixed paraffin-embedded (FFPE) tissues (27 clear cell, 29 papillary, 19 chromophobe, 4 unclassified RCC and 11 oncocytomas)...
January 5, 2018: Oncotarget
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