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miR-200b

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https://www.readbyqxmd.com/read/29317613/glucose-6-phosphate-dehydrogenase-is-indispensable-in-embryonic-development-by-modulation-of-epithelial-mesenchymal-transition-via-the-nox-smad3-mir-200b-axis
#1
Yi-Hsuan Wu, Ying-Hsuan Lee, Hung-Yu Shih, Shih-Hsiang Chen, Yi-Chuan Cheng, Daniel Tsun-Yee Chiu
Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme involved in the pentose phosphate shunt for producing nicotinamide adenine dinucleotide phosphate (NADPH). Severe G6PD deficiency leads to embryonic lethality, but the underlying mechanism is unclear. In the current study, the effects of G6PD on epithelial-mesenchymal transition (EMT), especially during embryonic development, were investigated. The knockdown of G6PD induced morphological changes, accompanied by the suppression of epithelial markers, E-cadherin and β-catenin, in A549 and MDCK cells...
January 9, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29303912/transforming-growth-factor-%C3%AE-and-bone-morphogenetic-protein-2-regulation-of-microrna-200-family-in-chronic-pancreatitis
#2
Peter Yu, Ka Liu, Xuxia Gao, Harry Karmouty-Quintana, Jennifer M Bailey, Yanna Cao, Tien C Ko
OBJECTIVES: To investigate regulation of microRNA (miR)-200 family (a, b, c, 141, and 429) in chronic pancreatitis (CP). This was accomplished by examining miR-200 family levels in a mouse model in vivo and their regulation in pancreatic cells in vitro. METHODS: Chronic pancreatitis was induced by cerulein for 4 weeks (50 μg/kg, 5 hourly intraperitoneal injections/day, and 3 days/week). Control mice received normal saline. The pancreata were harvested for fibrosis assessment by Sirius red staining and for miRNA, collagen, and fibronectin levels by quantitative PCR...
January 5, 2018: Pancreas
https://www.readbyqxmd.com/read/29258530/disruption-of-the-c-myc-mir-200b-3p-prdx2-regulatory-loop-enhances-tumor-metastasis-and-chemotherapeutic-resistance-in-colorectal-cancer
#3
Zhenbing Lv, Jinlai Wei, Wenxian You, Rong Wang, Jingkun Shang, Yongfu Xiong, Hua Yang, Xuanhua Yang, Zhongxue Fu
BACKGROUND: Metastasis is a major threat to colorectal cancer (CRC) patients. We have reported that peroxiredoxin-2 (PRDX2) is associated with CRC invasion and metastasis. However, the mechanisms regulating PRDX2 expression remain unclear. We investigate whether microRNAs (miRNAs) regulate PRDX2 expression in CRC progression. METHODS: Quantitative real-time polymerase chain reaction (qPCR) was used to measure microRNA-200b-3p (miR-200b-3p) expression. Immunohistochemistry (IHC) was performed to detect c-Myc and PRDX2 protein levels in CRC tissue samples (n = 97)...
December 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29249979/potential-contributions-of-mir-200a-200b-and-their-target-gene-leptin-to-the-sexual-size-dimorphism-in-yellow-catfish
#4
Jin Zhang, Wenge Ma, Yan He, Farman U Dawar, Shuting Xiong, Jie Mei
Sexual size dimorphism is the consequence of differential expression of sex-biased genes related to feeding and growth. Leptin is known to regulate energy balance by regulating food intake. In order to investigate the molecular mechanism of sexual size dimorphism in yellow catfish (Pelteobagrus fulvidraco), the expression of leptin (lep) and its functional receptor (lepr) were detected during larval development. Both lep and lepr have lower expression in males than in females during 1-4 weeks post hatching...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29202733/expression-of-micro-rnas-and-genes-related-to-angiogenesis-in-ccrcc-and-associations-with-tumor-characteristics
#5
Rita de Cássia Oliveira, Renato Fidelis Ivanovic, Katia Ramos Moreira Leite, Nayara Izabel Viana, Ruan César Aparecido Pimenta, José Pontes Junior, Vanessa Ribeiro Guimarães, Denis Reis Morais, Daniel Kanda Abe, Adriano João Nesrallah, Miguel Srougi, William Nahas, Sabrina Thalita Reis
BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is the third most common urological cancer in adults. Our aim is to evaluate genes and miRNAs expression profiles involved with angiogenesis and tumor characteristics in ccRCC. METHODS: The expression levels of miRNAs miR-99a, 99b, 100; 199a; 106a; 106b; 29a; 29b; 29c; 126; 200a, 200b and their respective target genes: mTOR, HIF1-α, VHL, PDGF, VEGF, VEGFR1 and VEGFR2 were analyzed using qRT-PCR in tumor tissue samples from 56 patients with ccRCC...
December 4, 2017: BMC Urology
https://www.readbyqxmd.com/read/29200203/mir-200b-c-attenuates-lipopolysaccharide-induced-early-pulmonary-fibrosis-by-targeting-zeb1-2-via-p38-mapk-and-tgf-%C3%AE-smad3-signaling-pathways
#6
Yongmei Cao, Yujing Liu, Feng Ping, Lyu Yi, Zhen Zeng, Yingchuan Li
Pulmonary fibrosis triggered during the early stage of acute respiratory distress syndrome (ARDS) contributes to poor prognosis in patients. However, whether microRNAs (miRNAs) can serve as therapeutic targets for early pulmonary fibrosis during ARDS is still largely unknown. In this study, we evaluated the effects and mechanisms of miR-200s and its targets ZEB1/2 in lung tissue. An early pulmonary fibrosis mouse model caused by ARDS was established via a lipopolysaccharide (LPS) three-hit regimen. Lentiviral packaged miR-200b/c cDNA or ZEB1/2 shRNA was intratracheally administered into the lungs of C57BL/6 mice 1 day before an LPS injection was administered...
December 4, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29167681/mir-200b-downregulates-cftr-during-hypoxia-in-human-lung-epithelial-cells
#7
Sylwia Bartoszewska, Wojciech Kamysz, Bogdan Jakiela, Marek Sanak, Jarosław Króliczewski, Zsuzsa Bebok, Rafal Bartoszewski, James F Collawn
Background: Hypoxic conditions induce the expression of hypoxia-inducible factors (HIFs) that allow cells to adapt to the changing conditions and alter the expression of a number of genes including the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is a low abundance mRNA in airway epithelial cells even during normoxic conditions, but during hypoxia its mRNA expression decreases even further. Methods: In the current studies, we examined the kinetics of hypoxia-induced changes in CFTR mRNA and protein levels in two human airway epithelial cell lines, Calu-3 and 16HBE14o-, and in normal primary bronchial epithelial cells...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29156719/the-micrornas-mir-200b-3p-and-mir-429-5p-target-the-limk1-cfl1-pathway-to-inhibit-growth-and-motility-of-breast-cancer-cells
#8
Dengfeng Li, Hong Wang, Hongming Song, Hui Xu, Bingkun Zhao, Chenyang Wu, Jiashu Hu, Tianqi Wu, Dan Xie, Junyong Zhao, Qiang Shen, Lin Fang
Triple-negative breast cancer (TNBC) has the worst prognosis of all subtypes of breast cancer (BC), with limited options for conventional therapy and no targeted therapies. MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression. In this study, we aimed to determine whether two members of the miR-200 family, miR-200b-3p and miR-429-5p, are involved in BC cell proliferation and motility and to elucidate their target genes and pathways. We performed a meta-analysis that reveals down-regulated expression of miR-200b-3p and miR-429-5p in BC tissues and cell lines, consistent with a lower expression of miR-200b-3p and miR-429-5p in MDA-MB-231 and HCC1937 cells than in MCF-7 and MCF-10 cells...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29150958/inhibition-of-mir-200b-mir-429-contributes-to-neuropathic-pain-development-through-targeting-zinc-finger-e-box-binding-protein-1
#9
Xue-Tao Yan, Ying Zhao, Xiao-Li Cheng, Xiang-Hu He, Yu Wang, Wen-Zhong Zheng, Hu Chen, Yan-Lin Wang
Many studies have reported that microRNAs participate in neuropathic pain development. Previously, miR-200b and miR-429 are reported to be involved in various diseases. In our current study, we focused on their roles in neuropathic pain and we found that miR-200b and miR-429 were significantly decreased in chronic constriction injury (CCI) rat spinal cords and isolated microglials. miR-200b and miR-429 overexpression were able to relieve neuropathic pain through modulating PWT and PWL in CCI rats. Meanwhile, we observed that both miR-200b and miR-429 upregulation could repress neuroinflammation via inhibiting inflammatory cytokines such as IL-6, IL-1β and TNF-α in CCI rats...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29137351/microrna-200-associated-with-metastatic-breast-cancer-promotes-traits-of-mammary-luminal-progenitor-cells
#10
Lourdes Sánchez-Cid, Mònica Pons, Juan José Lozano, Nuria Rubio, Marta Guerra-Rebollo, Aroa Soriano, Laia Paris-Coderch, Miquel F Segura, Raquel Fueyo, Judit Arguimbau, Erika Zodda, Raquel Bermudo, Immaculada Alonso, Xavier Caparrós, Marta Cascante, Arash Rafii, Yibin Kang, Marian Martínez-Balbás, Stephen J Weiss, Jerónimo Blanco, Montserrat Muñoz, Pedro L Fernández, Timothy M Thomson
MicroRNAs are critical regulators of gene networks in normal and abnormal biological processes. Focusing on invasive ductal breast cancer (IDC), we have found dysregulated expression in tumor samples of several microRNAs, including the miR-200 family, along progression from primary tumors to distant metastases, further reflected in higher blood levels of miR-200b and miR-7 in IDC patients with regional or distant metastases relative to patients with primary node-negative tumors. Forced expression of miR-200s in MCF10CA1h mammary cells induced an enhanced epithelial program, aldehyde dehydrogenase (ALDH) activity, mammosphere growth and ability to form branched tubuloalveolar structures while promoting orthotopic tumor growth and lung colonization in vivo...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29135495/prenatal-microrna-mir-200b-therapy-improves-nitrofen-induced-pulmonary-hypoplasia-associated-with-congenital-diaphragmatic-hernia
#11
Naghmeh Khoshgoo, Ramin Kholdebarin, Patricia Pereira-Terra, Thomas H Mahood, Landon Falk, Chelsea A Day, Barbara M Iwasiow, Fuqin Zhu, Drew Mulhall, Carly Fraser, Jorge Correia-Pinto, Richard Keijzer
OBJECTIVE: We aimed to evaluate the use of miR-200b as a prenatal transplacental therapy in the nitrofen rat model of abnormal lung development and congenital diaphragmatic hernia (CDH). BACKGROUND: Pulmonary hypoplasia (PH) and pulmonary hypertension determine mortality and morbidity in CDH babies. There is no safe medical prenatal treatment available. We previously discovered that higher miR-200b is associated with better survival in CDH babies. Here, we investigate the role of miR-200b in the nitrofen rat model of PH and CDH and evaluate its use as an in vivo prenatal therapy...
November 13, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29105802/circulating-micrornas-and-treatment-response-in-the-phase-ii-swog-s0925-study-for-patients-with-new-metastatic-hormone-sensitive-prostate-cancer
#12
Heather H Cheng, Melissa Plets, Hongli Li, Celestia S Higano, Catherine M Tangen, Neeraj Agarwal, Nicholas J Vogelzang, Maha Hussain, Ian M Thompson, Muneesh Tewari, Evan Y Yu
BACKGROUND: Previous studies suggest circulating, blood-based microRNAs (miRNAs) may serve as minimally invasive prostate cancer biomarkers, however there is limited data from prospective clinical trials. Here, we explore the role of candidate plasma miRNAs as potential biomarkers in the SWOG 0925 randomized phase II study of androgen deprivation combined with cixutumumab versus androgen deprivation alone in patients with new metastatic hormone-sensitive prostate cancer. METHODS: Correlative biospecimens, including circulating tumor cells (CTCs) and plasma for miRNA analysis, were collected at baseline and after 12 weeks on treatment from 50 patients enrolled on SWOG 0925...
November 6, 2017: Prostate
https://www.readbyqxmd.com/read/29095265/identification-of-differential-expressed-pe-exosomal-mirna-in-lung-adenocarcinoma-tuberculosis-and-other-benign-lesions
#13
Yan Wang, Yan-Mei Xu, Ye-Qing Zou, Jin Lin, Bo Huang, Jing Liu, Jing Li, Jing Zhang, Wei-Ming Yang, Qing-Hua Min, Shu-Qi Li, Qiu-Fang Gao, Fan Sun, Qing-Gen Chen, Lei Zhang, Yu-Huan Jiang, Li-Bin Deng, Xiao-Zhong Wang
Pleural effusion (PE) is a common clinical complication of many pulmonary and systemic diseases, including lung cancer and tuberculosis. Nevertheless, there is no clinical effective biomarker to identify the cause of PE. We attempted to investigate differential expressed exosomal miRNAs in PEs of lung adenocarcinoma (APE), tuberculous (TPE), and other benign lesions (NPE) by using deep sequencing and quantitative polymerase chain reaction (qRT-PCR). As a result, 171 differentiated miRNAs were observed in 3 groups of PEs, and 11 significantly differentiated exosomal miRNAs were validated by qRT-PCR...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29077234/dna-methylation-regulated-micrornas-in-human-cervical-cancer
#14
Vinay Koshy Varghese, Vaibhav Shukla, Shama Prasada Kabekkodu, Deeksha Pandey, Kapaettu Satyamoorthy
Regulation of miRNA gene expression by DNA promoter methylation may represent a key mechanism to drive cervical cancer progression. In order to understand the impact of DNA promoter methylation on miRNAs at various stages of cervical carcinogenesis, we performed DNA methylation microarray on Normal Cervical Epithelium (NCE), Cervical Intraepithelial Neoplasia (CIN I - III) and Squamous Cell Carcinoma (SCC) tissues to identify differentially methylated miRNAs followed by validation by bisulfite sequencing. Further, expression of miRNAs was analyzed by qRT-PCR in clinical tissues and cervical cancer cell lines...
October 27, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29064597/mir-23b-3p-and-mir-125b-5p-downregulate-apo-a-expression-by-targeting-ets1-in-hepg2-cells
#15
Jun-Fa Zeng, Zhao-Lin Zeng, Kai Zhang, Yue Zhao, Ya-Mi Liu, Jiao-Jiao Chen, Hai Tong, Dang-Heng Wei, Zhi-Sheng Jiang, Zuo Wang
High concentrations of plasma lipoprotein(a) [Lp(a)] have been inferred to be an independent risk factor for cardiovascular and cerebrovascular diseases, such as coronary artery diseases, restenosis, and stroke. Apolipoprotein(a) [apo(a)] is one of the most important components of Lp(a) and contributes greatly to the increased concentration of plasma Lp(a). As a critical positive transacting factor of apo(a) gene, Ets1 has been proven as a target gene of several miRNAs, such as miR-193b, miR-125b-5p, miR-200b, miR-1, and miR-499...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29050269/long-non-coding-rna-carlo-5-promotes-tumor-progression-in-hepatocellular-carcinoma-via-suppressing-mir-200b-expression
#16
Chunqing Dou, Liyuan Sun, Xin Jin, Mingming Han, Bao Zhang, Xian Jiang, Jinyong Lv, Tao Li
Long non-coding RNAs (lncRNAs) play key roles in cancer initiation and progression. The aim was to investigate the biological functions and clinical significance of long non-coding RNA CARLo-5 in hepatocellular carcinoma (HCC). QRT-PCR was performed to investigate CARLo-5 expression in HCC tissues and cells. Kaplan-Meier curve and multivariate analysis validated the association between CARLo-5 expression and overall survival (OS) in HCC patients. Cell proliferation and invasion was performed by CCK8 cell proliferation, cell colony formation and transwell invasion assays...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29047185/pluripotent-stem-cell-derived-haematopoietic-progenitors-are-unable-to-downregulate-key-emt-associated-mirnas
#17
Ellie Meader, Tomas Barta, Dario Melguzo-Sanchis, Katarzyna Tilgner, David Montaner, Ashraf A El-Harouni, Lyle Armstrong, Majlinda Lako
Haematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited, with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression, and are responsible for their failure to engraft...
October 19, 2017: Stem Cells
https://www.readbyqxmd.com/read/29042072/mir-200b-downregulates-kruppel-like-factor-2-klf2-during-acute-hypoxia-in-human-endothelial-cells
#18
Rafal Bartoszewski, Marcin Serocki, Anna Janaszak-Jasiecka, Sylwia Bartoszewska, Kinga Kochan-Jamrozy, Arkadiusz Piotrowski, Jarosław Króliczewski, James F Collawn
The role of microRNAs in controlling angiogenesis is recognized as a promising therapeutic target in both cancer and cardiovascular disorders. However, understanding a miRNA's pleiotropic effects on angiogenesis is a limiting factor for these types of therapeutic approaches. Using genome-wide next-generation sequencing, we examined the role of an antiangiogenic miRNA, miR-200b, in primary human endothelial cells. The results indicate that miR-200b has complex effects on hypoxia-induced angiogenesis in human endothelia and importantly, that many of the reported miR-200b effects using miRNA overexpression may not be representative of the physiological role of this miRNA...
December 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/29037594/epigenetic-modification-of-microrna-200b-contributes-to-diabetic-vasculopathy
#19
Kanhaiya Singh, Durba Pal, Mithun Sinha, Subhadip Ghatak, Surya C Gnyawali, Savita Khanna, Sashwati Roy, Chandan K Sen
Hyperglycemia (HG) induces genome-wide cytosine demethylation. Our previous work recognized miR-200b as a critical angiomiR, which must be transiently downregulated to initiate wound angiogenesis. Under HG, miR-200b downregulation is not responsive to injury. Here, we demonstrate that HG may drive vasculopathy by epigenetic modification of a miR promoter. In human microvascular endothelial cells (HMECs), HG also lowered DNA methyltransferases (DNMT-1 and DNMT-3A) and compromised endothelial function as manifested by diminished endothelial nitric oxide (eNOS), lowered LDL uptake, impaired Matrigel tube formation, lower NO production, and compromised VE-cadherin expression...
September 12, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29032608/microrna-profiling-reveals-dysregulated-micrornas-and-their-target-gene-regulatory-networks-in-cemento-ossifying-fibroma
#20
Thaís Dos Santos Fontes Pereira, João Artur Ricieri Brito, André Luiz Sena Guimarães, Carolina Cavaliéri Gomes, Júlio Cesar Tanos de Lacerda, Wagner Henriques de Castro, Roney Santos Coimbra, Marina Gonçalves Diniz, Ricardo Santiago Gomez
BACKGROUND: Cemento-ossifying fibroma (COF) is a benign fibro-osseous neoplasm of uncertain pathogenesis, and its treatment results in morbidity. MicroRNAs (miRNA) are small non-coding RNAs that regulate gene expression and may represent therapeutic targets. The purpose of the study was to generate a comprehensive miRNA profile of COF compared to normal bone. Additionally, the most relevant pathways and target genes of differentially expressed miRNA were investigated by in silico analysis...
October 15, 2017: Journal of Oral Pathology & Medicine
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