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miR-200b

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https://www.readbyqxmd.com/read/28548126/microrna-dysregulation-in-the-tumor-microenvironment-influences-the-phenotype-of-pancreatic-cancer
#1
Eva Karamitopoulou, Stefan Haemmig, Ulrich Baumgartner, Cornelia Schlup, Martin Wartenberg, Erik Vassella
Cellular interactions in the tumor microenvironment influence neoplastic progression in pancreatic ductal adenocarcinoma. One underlying mechanism is the induction of the prognostically unfavorable epithelial-mesenchymal-transition-like tumor budding. Our aim is to explore the expression of microRNAs implicated in the regulation of tumor budding focusing on the microenvironment of the invasive front. To this end, RNA from laser-capture-microdissected material of the main tumor, tumor buds, juxta-tumoral stroma, tumor-remote stroma, and non-neoplastic pancreatic parenchyma from pancreatic cancer cases with (n=7) and without (n=6) tumor budding was analyzed by qRT-PCR for the expression of a panel of miRNAs that are known to be implicated in the regulation of epithelial-mesenchymal transition, including miR-21, miR-183, miR-200b, miR-200c, miR-203, miR-205, miR-210, and miR-217...
May 26, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28535666/ddx53-regulates-cancer-stem-cell-like-properties-by-binding-to-sox-2
#2
Youngmi Kim, Minjeong Yeon, Dooil Jeoung
This study investigated the role of cancer/testis antigen DDX53 in regulating cancer stem cell-like properties. DDX53 shows co-expression with CD133, a marker for cancer stem cells. DDX53 directly regulates the SOX-2 expression in anticancer drug-resistant Malme3MR cells. DDX53 and miR-200bwere found to be involved in the regulation of tumor spheroid forming potential of Malme3M and Malme3MR cells. Furthermore, the self-renewal activity and the tumorigenic potential of Malme3M(R)-CD133 (+) cells were also regulated by DDX53...
May 2, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28535509/erythropoietin-inhibits-hypoxia-induced-epithelial-to-mesenchymal-transition-via-upregulation-of-mir-200b-in-hk-2-cells
#3
Jiuxu Bai, Xiao Xiao, Xiaoling Zhang, Hanmin Cui, Junfeng Hao, Jingming Han, Ning Cao
BACKGROUND/AIMS: Renal tubular epithelial-mesenchymal transition (EMT) is regarded as an important factor leading to renal interstitial fibrosis. Erythropoietin (EPO) has been reported to attenuate renal fibrosis. The mechanism underlying this protective effect of EPO remains unclear. In this study, we aim to identify possible mechanisms of the EPO renoprotective effect. METHODS: Hypoxia was induced in vitro by incubating human proximal tubular epithelial cell line HK-2 cells in 1% O2 and 5% CO2...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28529604/dicer1-regulates-endometrial-carcinoma-invasion-via-histone-acetylation-and-methylation
#4
Bilan Li, Wen Lu, Junjie Qu, Yongli Zhang, Xiaoping Wan
Endometrial carcinoma (EC) is one of the most common gynecologic malignancy, but molecular mechanisms of the development and progression of EC remain unclear. Here we showed that the expression of DICER1 was negatively associated with the level of histone methylation, histone acetylation and PRC2 components SUZ12 and EZH2 in EC cells. In addition, knockdown of DICER1 significantly downregulated miR-200b and let-7i, which may then regulate their targets SUZ12 and EZH2. Furthermore, knockdown of DICER1 remarkably suppressed the expression of epithelial cell marker E-cadherin, induced the expression of mesenchymal cell marker Vimentin, and promoted the invasion of EC cells...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28507337/identification-and-comparative-analysis-of-the-ovarian-micrornas-of-prolific-and-non-prolific-goats-during-the-follicular-phase-using-high-throughput-sequencing
#5
Xiang-Dong Zi, Jian-Yuan Lu, Li Ma
The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present study provides the first comparison of the ovarian miRNAs of prolific Jintang black goats (JTGs) and non-prolific Tibetan goats (TBGs) during the follicular phase using RNA-Seq technology. We generated 11.19 million (M) and 11...
May 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28502477/knockdown-of-hepatic-gonadotropin-releasing-hormone-by-vivo-morpholino-decreases-liver-fibrosis-in-multidrug-resistance-gene-2-knockout-mice-by-down-regulation-of-mir-200b
#6
Konstantina Kyritsi, Fanyin Meng, Tianhao Zhou, Nan Wu, Julie Venter, Heather Francis, Lindsey Kennedy, Paolo Onori, Antonio Franchitto, Francesca Bernuzzi, Pietro Invernizzi, Kelly McDaniel, Romina Mancinelli, Domenico Alvaro, Eugenio Gaudio, Gianfranco Alpini, Shannon Glaser
Hepatic fibrosis occurs during the progression of primary sclerosing cholangitis (PSC) and is characterized by accumulation of extracellular matrix proteins. Proliferating cholangiocytes and activated hepatic stellate cells (HSCs) participate in the promotion of liver fibrosis during cholestasis. Gonadotropin-releasing hormone (GnRH) is a trophic peptide hormone synthesized by hypothalamic neurons and the biliary epithelium and exerts its biological effects on cholangiocytes by interaction with the receptor subtype (GnRHR1) expressed by cholangiocytes and HSCs...
May 11, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28490276/endometrial-stromal-and-epithelial-cells-exhibit-unique-aberrant-molecular-defects-in-patients-with-endometriosis
#7
Philip C Logan, Pamela Yango, Nam D Tran
CONTEXT: Endometriosis is a chronic inflammatory disease that causes pain and infertility in women of reproductive age. OBJECTIVE: To investigate the pathologic pathways in endometrial stromal and epithelial cells that contribute to the manifestation of endometriosis. DESIGN: In vitro cellular and molecular analyses of isolated eutopic endometrial stromal and epithelial cells. METHODS: Eutopic stromal and epithelial cells from endometriotic and normal patients were isolated by fluorescence-activated cell sorting for paired sibling RNA sequencing and microRNA microarray...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28487474/long-noncoding-rna-heih-promotes-melanoma-cell-proliferation-migration-and-invasion-via-inhibiting-mir-200b-a-429
#8
Haiying Zhao, Guoping Xing, Yingying Wang, Zengxiang Luo, Guoyan Liu, Huijuan Meng
Long noncoding RNAs (lncRNAs) are frequently dysregulated and have important roles in many diseases, particular in cancers. lncRNA-HEIH was first identified in hepatocellular carcinoma. The expression, clinical significances and roles of lncRNA-HEIH in melanoma are still unknown. In this study, we found that lncRNA-HEIH is highly expressed in melanoma tissues and cell lines, associated with advanced clinical stages, and predicts poor outcome of melanoma patients. Functional assays showed that ectopic expression of lncRNA-HEIH promotes melanoma cell proliferation, migration and invasion...
May 9, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28472720/epithelial-to-mesenchymal-transition-in-cyclosporine-a-induced-rat-gingival-overgrowth
#9
Fei Yang, Jing Lu, Youcheng Yu, Yiming Gong
BACKGROUND AND OBJECTIVE: Epithelial-mesenchymal transition (EMT) has been proved to occur in drug-induced gingival overgrowth. However, the specific pathogenic mechanism remains uncertain. The aim of this study is to examine the expression of EMT markers in cyclosporine A (CsA)-induced gingival overgrowth in rat models. MATERIAL AND METHODS: Thirty-six rats were randomly divided into two groups. The experimental group received CsA therapy subcutaneously in a daily dose of 10mg/kg, and the other group was used as a control...
April 23, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28451393/the-circulating-microrna-200-family-in-whole-blood-are-potential-biomarkers-for-high-grade-serous-epithelial-ovarian-cancer
#10
Adam Pendlebury, Natalie J Hannan, Natalie Binder, Sally Beard, Monica Mcgauran, Peter Grant, Stephen Tong, Clare L Whitehead
Epithelial ovarian cancer (EOC) is the leading cause of mortality with regard to gynaecological cancer. There is no effective biomarker and therefore prognosis is poor as the majority of cases are not diagnosed until advanced disease is present. MicroRNAs (miRs) are dysregulated in ovarian cancer tissue and are present in the circulation. The aim of the present study was to investigate whether circulating miRs from the miR-200 family served as potential candidate biomarkers for the early detection of EOC. Whole blood and ovarian tissue were collected from women with early (stage I/II, n=4), advanced EOC (stage III/IV, n=4), and women with benign ovarian masses (n=5)...
March 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28446464/trf-mir-1280-suppresses-stem-cell-like-cells-and-metastasis-in-colorectal-cancer
#11
Bingqing Huang, Huipeng Yang, Xixi Cheng, Dan Wang, Shuyu Fu, Wencui Shen, Qi Zhang, Lijuan Zhang, Zhenyi Xue, Yan Li, Yurong Da, Qing Yang, Zesong Li, Li Liu, Liang Qiao, Ying Kong, Zhi Yao, Peng Zhao, Min Li, Rongxin Zhang
Several studies have shown that tRNAs can be enzymatically cleaved to generate distinct classes of tRNA-derived fragments (tRF). Here we report that tRF/miR-1280, a 17bp fragment derived from tRNA(Leu) and pre-miRNA, influences Notch signaling pathways that support the function of cancer stem-like cells (CSC) in colorectal cancer progression. tRF/miR-1280 expression was decreased in human specimens of colorectal cancer (CRC). Ectopic expression of tRF/miR-1280 reduced cell proliferation and colony formation, whereas its suppression reversed these effects...
April 26, 2017: Cancer Research
https://www.readbyqxmd.com/read/28413462/identification-of-mirna-biomarkers-of-pneumonia-using-rna-sequencing-and-bioinformatics-analysis
#12
Sai Huang, Cong Feng, Yong-Zhi Zhai, Xuan Zhou, Bei Li, Li-Li Wang, Wei Chen, Fa-Qin Lv, Tan-Shi Li
Pneumonia is a lower respiratory tract infection that causes dramatic mortality worldwide. The present study aimed to investigate the pathogenesis of pneumonia and identify microRNA (miRNA) biomarkers as candidates for targeted therapy. RNA from the peripheral blood plasma of participants with pneumonia (severe, n=9; non-severe, n=9) and controls (n=9) was isolated and paired-end sequencing was performed on an Illumina HiSeq4000 system. Following the processing of raw reads, the sequences were aligned against the Genome Reference Consortium human genome assembly 38 reference genome using Bowtie2 software...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28411194/systematic-characterization-of-a-to-i-rna-editing-hotspots-in-micrornas-across-human-cancers
#13
Yumeng Wang, Xiaoyan Xu, Shuangxing Yu, Kang Jin Kang, Zhicheng Zhou, Leng Han, Yiu Huen Tsang, Jun Li, Hu Chen, Lingegowda S Mangala, Yuan Yuan, A Karina Eterovic, Yiling Lu, Anil K Sood, Kenneth L Scott, Gordon B Mills, Han Liang
RNA editing, a widespread posttranscriptional mechanism, has emerged as a new player in cancer biology. Recent studies have reported key roles for individual miRNA editing events, but a comprehensive picture of miRNA editing in human cancers remains largely unexplored. Here we systematically characterized the miRNA editing profiles of 8,595 samples across 20 cancer types from miRNA sequencing data of The Cancer Genome Atlas and identified 19 adenosine-to-inosine (A-to-I) RNA editing hotspots. We independently validated 15 of them by perturbation experiments in several cancer cell lines...
April 14, 2017: Genome Research
https://www.readbyqxmd.com/read/28402940/prognostic-role-of-micrornas-in-human-gastrointestinal-cancer-a-systematic-review-and-meta-analysis
#14
REVIEW
Qiang Zheng, Changyu Chen, Haiyang Guan, Weibiao Kang, Changjun Yu
BACKGROUND: Gastrointestinal cancers (GICs) mainly including esophageal, gastric and colorectal cancer, are the most common cause of cancer-related death and lead into high mortality worldwide. We performed this systematic review and meta-analysis to elucidate relationship between multiple microRNAs (miRs) expression and survival of GIC patients. METHODS: We searched a wide range of database. Fixed-effects and random-effects models were used to calculate the pooled hazard ratio values of overall survival and disease free survival...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28399108/decreased-levels-of-baseline-and-drug-induced-tubulin-polymerisation-are-hallmarks-of-resistance-to-taxanes-in-ovarian-cancer-cells-and-are-associated-with-epithelial-to-mesenchymal-transition
#15
George E Duran, Yan C Wang, François Moisan, E Brian Francisco, Branimir I Sikic
BACKGROUND: ABCB1 expression is uncommon in ovarian cancers in the clinical setting so we investigated non-MDR mechanisms of resistance to taxanes. METHODS: We established eight taxane-resistant variants from the human ovarian carcinoma cell lines A2780/1A9, ES-2, MES-OV and OVCAR-3 by selection with paclitaxel or docetaxel, with counter-selection by the transport inhibitor valspodar. RESULTS: Non-MDR taxane resistance was associated with reduced intracellular taxane content compared to parental controls, and cross-resistance to other microtubule stabilising drugs...
May 9, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28383782/the-mir-200b-zeb1-circuit-regulates-diverse-stemness-of-human-hepatocellular-carcinoma
#16
Shu-Chun Tsai, Chen-Chun Lin, Tsung-Chieh Shih, Rong-Jeng Tseng, Ming-Chin Yu, Yu-Jr Lin, Sen-Yung Hsieh
Accumulating evidence suggests that human hepatocellular carcinoma (HCC) can be derived from cancer stem cells (CSCs), which contribute to tumor initiation, metastasis, chemoresistance, and recurrence. A great variety of HCC CSCs resulting in diverse clinical manifestations have been reported. However, how CSC diversity is regulated and generated remains unclear. Here we report that the miR-200b-ZEB1 circuit is closely involved with the induction and maintenance of a diverse group of CSCs. We found that miR-200b downregulation occurred in early HCC and associated with poor prognosis...
April 6, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28370348/microvesicles-shuttled-mir-200b-attenuate-experimental-colitis-associated-intestinal-fibrosis-by-inhibiting-the-development-of-emt
#17
Jia Yang, Cheng-Zhi Zhou, Rui Zhu, Heng Fan, Xing-Xing Liu, Xue-Yun Duan, Qing Tang, Zhe-Xing Shou, Dong-Mei Zuo
BACKGROUND: Epithelial-mesenchymal transition (EMT), characterized by the decrease of E-Cad and increase of vimentin and α-SMA, was demonstrated to participate in inflammatory bowel disease (IBD) related fibrosis. MiR-200b plays an anti-fibrosis role in inhibiting EMT by targeting ZEB1 and ZEB2. But the stability of exogenous miR-200b in blood limits its application. Microvesicles (MVs), which can transfer miRNAs among cells and prevent them from degradation, may provide an excellent transport system for the delivery of miR-200b in the treatment of fibrosis...
March 31, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28362995/targeting-effect-of-microrna-on-cd133-and-its-impact-analysis-on-proliferation-and-invasion-of-glioma-cells
#18
C Zhao, Z G Ma, S L Mou, Y X Yang, Y H Zhang, W C Yao
MiR-200b, a member of the microRNA-200 family, has been identified to be capable of suppressing glioma cell growth through targeting CREB1 or CD133. However, whether miR-200b affects the biological behavior (proliferation, invasion, and migration) of glioma cells is poorly understood. The aim of this study was to evaluate the effect of miR-200b on the biological behavior of glioma cells in vitro. MiRNA-200b mimics, miRNA-200b inhibitor, and mimic control were transfected into conventionally cultured glioma U251 cells, followed by measuring the expression of miR-200b and CD133 in transfected cells by RT-PCR; effect of miR-200b on CD133 mRNA 3'-UTR luciferase activity by luciferase reporter assay; proliferation activity of transfected U251 cells by MTT method; and changes in U251 cell invasion and migration by Transwell method after transfection...
March 30, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28347920/mir-200b-and-mir-155-as-predictive-biomarkers-for-the-efficacy-of-chemoradiation-in-locally-advanced-head-and-neck-squamous-cell-carcinoma
#19
Anne-Katrin Hess, Annika Müer, Fabian Dominik Mairinger, Wilko Weichert, Albrecht Stenzinger, Michael Hummel, Volker Budach, Ingeborg Tinhofer
BACKGROUND: The predictive value of microRNAs (miRNAs) in tumour cells and infiltrating immune cells for the efficacy of chemoradiation (CRTX) in locally advanced head and neck squamous cell carcinoma (HNSCC) was evaluated. METHODS: Formalin-fixed, paraffin-embedded tumour material was collected from patients with locally advanced HNSCC treated within the ARO-0401 phase III trial with radiotherapy in combination with either 5-fluorouracil/cisplatin (CDDP-CRTX) or 5-fluorouracil/mitomycin C (MMC-CRTX)...
March 24, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28345813/effect-of-the-interaction-between-mir-200b-3p-and-dnmt3a-on-cartilage-cells-of-osteoarthritis-patients
#20
Jian Wu, Yunjuan Tao, Anquan Shang, Weiwei Wang, Yujie Zhang, Liqing Hu, Jun Wang, Yuan Wang, Naizhou Guo
The aim of this research is to explore the effect of miR-200b-3p targeting DNMT3A on the proliferation and apoptosis of osteoarthritis (OA) cartilage cells. Quantitative RT-PCR was performed to analyse the expression of miR-200b-3p, DNMT3A, MMP1, MMP3, MMP9, MMP13 and COL II in normal and OA cartilage tissues. The dual-luciferase reporter assay and Western blot assay were conducted to confirm the targeting relationship between miR-200b-3p and DNMT3A. We also constructed eukaryotic expression vector to overexpress miR-200b-3p and DNMT3A...
March 27, 2017: Journal of Cellular and Molecular Medicine
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