Natalie N Kinloch, Yanqin Ren, Winiffer D Conce Alberto, Winnie Dong, Pragya Khadka, Szu Han Huang, Talia M Mota, Andrew Wilson, Aniqa Shahid, Don Kirkby, Marianne Harris, Colin Kovacs, Erika Benko, Mario A Ostrowski, Perla M Del Rio Estrada, Avery Wimpelberg, Christopher Cannon, W David Hardy, Lynsay MacLaren, Harris Goldstein, Chanson J Brumme, Guinevere Q Lee, Rebecca M Lynch, Zabrina L Brumme, R Brad Jones
The Intact Proviral DNA Assay (IPDA) was developed to address the critical need for a scalable method for intact HIV-1 reservoir quantification. This droplet digital PCR-based assay simultaneously targets two HIV-1 regions to distinguish genomically intact proviruses against a large background of defective ones, and its application has yielded insights into HIV-1 persistence. Reports of assay failures however, attributed to HIV-1 polymorphism, have recently emerged. Here, we describe a diverse North American cohort of people with HIV-1 subtype B, where the IPDA yielded a failure rate of 28% due to viral polymorphism...
January 8, 2021: Nature Communications