keyword
MENU ▼
Read by QxMD icon Read
search

G1/S transition

keyword
https://www.readbyqxmd.com/read/28820316/cell-cycle-related-metabolism-and-mitochondrial-dynamics-in-a-replication-competent-pancreatic-beta-cell-line
#1
Chiara Montemurro, Suryakiran Vadrevu, Tatyana Gurlo, Alexandra E Butler, Kenny E Vongbunyong, Anton Petcherski, Orian S Shirihai, Leslie S Satin, Daniel Braas, Peter C Butler, Slavica Tudzarova
Cell replication is a fundamental attribute of growth and repair in multicellular organisms. Pancreatic beta-cells in adults rarely enter cell cycle, hindering the capacity for regeneration in diabetes. Efforts to drive beta-cells into cell cycle have so far largely focused on regulatory molecules such as cyclins and cyclin-dependent kinases (CDKs). Investigations in cancer biology have uncovered that adaptive changes in metabolism, the mitochondrial network, and cellular Ca(2+) are critical for permitting cells to progress through the cell cycle...
August 18, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28811550/application-of-galangin-an-active-component-of-alpinia-officinarum-hance-zingiberaceae-for-use-in-drug-eluting-stents
#2
Jung-Jin Lee, Ji-Hye Lee, Nam-Hui Yim, Joo-Hui Han, Jin Yeul Ma
In clinical pathology, stent interposition is used to treat vascular disease but can lead to restenosis. Drug-eluting stents (DES) are most commonly used to suppress restenosis but can also have side effects. Therefore, we investigated the anti-proliferative effect and its possible target in vitro and in vivo. We found that Alpinia officinarum Hance (AO) extract efficiently inhibited VSMC proliferation by arresting the transition from the G0/G1 to the S phase via the up-regulation of p27(KIP1) expression. Galangin (GA) was determined to be a significant component of this extract, with the same anti-proliferative activity as the raw extract...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28802234/paqr3-overexpression-suppresses-the-aggressive-phenotype-of-esophageal-squamous-cell-carcinoma-cells-via-inhibition-of-erk-signaling
#3
Ge Bai, Jianhu Chu, Mayinur Eli, Yongxing Bao, Hao Wen
Progestin and adipoQ receptor family member 3 (PAQR3) has exhibited anticancer activity in multiple malignancies. However, its expression and function in esophageal squamous cell carcinoma (ESCC) is still elusive. In this work, we examined the expression of PAQR3 in 40 surgically resected ESCC specimens and their adjacent normal tissues. The expression of PAQR3 in ESCC cell lines was measured after treatment with the demethylating agent 5-aza-2'-deoxycytidine (5-Aza-CdR). The effects of overexpression of PAQR3 on cell proliferation, colony formation, invasion, and tumorigenesis were investigated...
August 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28800675/discovery-and-pharmacological-characterisation-of-a-novel-series-of-highly-selective-inhibitors-of-cyclin-dependent-kinases-4-and-6-as-anticancer-agents
#4
Solomon Tadesse, Laychiluh Bantie, Khamis Tomusange, Mingfeng Yu, Saiful Islam, Nataliya Bykovska, Benjamin Noll, Ge Zhu, Peng Li, Frankie Lam, Malika Kumarasiri, Robert Milne, Shudong Wang
BACKGROUND AND PURPOSE: Cyclin D dependent kinases 4 and 6 (CDK4/6) are crucial regulators of the G1 to S phase transition of the cell cycle, and are actively pursued as therapeutic targets in cancer targets. We sought to discover a novel series of orally bioavailable, and highly selective small molecule inhibitors of CDK4/6. EXPERIMENTAL APPROACH: The discovery of pharmacological inhibitors and optimisation for potency, selectivity and drug properties were achieved by iterative chemical synthesis, biochemical screening against a panel of kinases, cell-based assays measuring cellular viability, cell cycle distribution, induction of apoptosis, and the level of retinoblastoma tumour suppressor protein (Rb) phosphorylation and E2 factor (E2F) regulated gene expression, and in vitro biopharmaceutical and in vivo pharmacokinetic profiling...
August 11, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28800532/cell-cycle-arrest-mediated-by-cd-induced-dna-damage-in-arabidopsis-root-tips
#5
Weina Cui, Hetong Wang, Jie Song, Xia Cao, Hilary J Rogers, Dennis Francis, Chunyun Jia, Lizong Sun, Meifang Hou, Yuesuo Yang, Peidong Tai, Wan Liu
Accumulating evidence demonstrates that the aberrant expression of cell cycle regulation and DNA repair genes can result in abnormal cell proliferation and genomic instability in eukaryotic cells under different stresses. Herein, Arabidopsis thaliana (Arabidopsis) seedlings were grown hydroponically on 0.5 × MS media containing cadmium (Cd) at 0-2.5mgL(-1) for 5d of treatment. Real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that expression of DNA damage repair and cell cycle regulation genes, including BRCA1, MRE11, WEE1, CDKA;1 and PCNA1, showed an inverted U-shaped dose-response...
August 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28797103/effects-of-cancer-testis-antigen-tfdp3-on-cell-cycle-regulation-and-its-mechanism-in-l-02-and-hepg2-cell-lines-in-vitro
#6
Yunshen Jiao, Lingyu Ding, Ming Chu, Tieshan Wang, Jiarui Kang, Xiaofan Zhao, Huanhuan Li, Xi Chen, Zirui Gao, Likai Gao, Yuedan Wang
TFDP3, also be known as HCA661, was one of the cancer-testis antigens, which only expressed in human tissues. The recent researches about TFDP3 mostly focused on its ability to control the drug resistance and apoptosis of tumor cells. However, the role of TFDP3 in the progress of the cell cycle is rarely involved. In this study, we examined the expression of TFDP3 in human liver tissues firstly. After that, we detect the expression of TFDP3 at the RNA level and protein level in L-02 cell line and HepG2 cell line, and the location of TFDP3 was defined by immunofluorescence technique...
2017: PloS One
https://www.readbyqxmd.com/read/28791382/potential-role-of-zeb1-as-a-dna-repair-regulator-in-colorectal-cancer-cells-revealed-by-cancer-associated-promoter-profiling
#7
Miao Wang, Su-Fei He, Lei-Lei Liu, Xiao-Xia Sun, Fan Yang, Qian Ge, Wei-Kang Wong, Jing-Yan Meng
Besides being a key contributor to epithelial‑to‑mesenchymal transition (EMT) activation and stemness maintenance, zinc finger E-box binding homeobox 1 (ZEB1) is also a crucial inducer of chemoresistance and radioresistance. Unlike the clear mechanism that mediates its effect on EMT and dedifferentiation, the mechanism of how ZEB1 promotes chemo- and radio-resistance remains to be elucidated. It has been previously reported that ZEB1 promotes DNA double-strand break clearance by enhancing the deubiquitylating activity of ubiquitin-specific peptidase (USP)7 on checkpoint kinase 1, which is an important step during DNA repair...
August 8, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28774850/-ru-pipe-dppb-bipy-pf6-a-novel-ruthenium-complex-that-effectively-inhibits-erk-activation-and-cyclin-d1-expression-in-a549-cells
#8
Guilherme A Ferreira-Silva, Marina M Ortega, Marco A Banionis, Graciana Y Garavelli, Felipe T Martins, Julia S M Dias, Cláudio Viegas, Jaqueline C de Oliveira, Fabio B do Nascimento, Antonio C Doriguetto, Marilia I F Barbosa, Marisa Ionta
Lung cancer is the most frequent type of cancer worldwide. In Brazil, only 14% of the patients diagnosed with lung cancer survived 5years in the last decades. Although improvements in the therapeutic approach, it is relevant to identify new chemotherapeutic agents. In this framework, ruthenium metal compounds emerge as a promising alternative to platinum-based compounds once they displayed lower cytotoxicity and more selectivity for tumor cells. The present study aimed to evaluate the antitumor potential of innovative ruthenium(II) complex, [Ru(pipe)(dppb)(bipy)]PF6 (PIPE) on A549 cells, which is derived from non-small cell lung cancer...
August 1, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28770829/wnk1-is-required-for-proliferation-induced-by-hypotonic-challenge-in-rat-vascular-smooth-muscle-cells
#9
Ya-Juan Zhang, Hua-Qing Zheng, Bao-Yi Chen, Lu Sun, Ming-Ming Ma, Guan-Lei Wang, Yong-Yuan Guan
Hypotonic challenge evoked vascular cell proliferation through activation of volume-regulated Cl(-) channel (VRCC), leading to a decrease in the intracellular Cl(-) concentration ([Cl(-)]i). We hypothesize that the decrease in [Cl(-)]i may activate one or several Cl(-)-sensitive kinases, resulting in a subsequent signaling cascade. In this study we demonstrated that WNK1, a Cl(-)-sensitive kinase, was involved in VRCC-induced proliferative signaling pathway in A10 vascular smooth muscle cells in vitro. A10 cells were exposed to a hypotonic challenge (225 mosmol·kg(-1)·H20), which caused significantly increase in WNK1 phosphorylation without altering WNK1 protein expression...
August 3, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28768199/loss-of-brap-results-in-premature-g1-s-phase-transition-and-impeded-neural-progenitor-differentiation
#10
Alison A Lanctot, Yan Guo, Yicong Le, Brittany M Edens, Richard S Nowakowski, Yuanyi Feng
Cells initiate fate decisions during G1 phase by converting extracellular signals into distinctive cell cycle kinetics. The DNA replication timing is determined in G1 phase; lengthened G1 and hastened S phases correlate with increased neurogenic propensity of neural progenitor cells (NPCs), although the underlying molecular control remains elusive. Here, we report that proper G1 phase completion in NPCs requires Brap, a Ras-Erk signaling modulator with ubiquitin E3 ligase activity. We identified Skp2 and Skp2-associated SCF ubiquitin ligase as a key target of Brap-mediated polyubiquitination...
August 1, 2017: Cell Reports
https://www.readbyqxmd.com/read/28763625/microrna-1-3p-inhibits-proliferation-and-migration-of-oral-squamous-cell-carcinoma-cells-by-targeting-dkk1
#11
Zhenshi Wang, Jiaolong Wang, Zhihua Chen, Kun Wang, Lianshui Shi
We aimed to investigate the functional role and mechanism of miR-1-3p and DKK1 in oral squamous cell carcinoma (OSCC) cells. The level of miR-1-3p and DKK1 expression were detected in OSCC tissues and cells using RT-qPCR and Western blot. Dual luciferase reporter gene assay was applied to confirm the targeting relationship between miR-1-3p and DKK1. Functional assays, including MTT, Transwell, colony formation and flow cytometric analysis, were conducted to verify their effect on cell progressions. MTT, colony formation and Transwell assay indicated that proliferation, migration and invasion of SCC-4 cells was impaired with high miR-1-3p expression but promoted with high DKK1 expression...
August 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28759023/mecp2-a-target-of-mir-638-facilitates-gastric-cancer-cell-proliferation-through-activation-of-the-mek1-2-erk1-2-signaling-pathway-by-upregulating-git1
#12
L Y Zhao, D D Tong, M Xue, H L Ma, S Y Liu, J Yang, Y X Liu, B Guo, L Ni, L Y Liu, Y N Qin, L M Wang, X G Zhao, C Huang
Methyl-CpG binding protein 2 (MeCP2) is involved in the carcinogenesis and progression of multiple types of cancer. However, its precise role in gastric cancer (GC) and the relevant molecular mechanism remain unknown. In the present study, we found that miR-638 levels were lower in GC tissues and GC cell lines than in adjacent normal tissues and normal gastric epithelial cell lines, respectively. Low miR-638 levels were associated with poor tumor differentiation, tumor size and lymph node metastasis. MeCP2 expression levels were higher in GC tissues than in adjacent normal tissues...
July 31, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28754843/hormone-induced-dna-damage-response-and-repair-mediated-by-cyclin-d1-in-breast-and-prostate-cancer
#13
REVIEW
Gabriele Di Sante, Agnese Di Rocco, Claudia Pupo, Mathew C Casimiro, Richard G Pestell
Cell cycle control proteins govern events that leads to the production of two identical daughter cells. Distinct sequential temporal phases, Gap 1 (G1), Gap 0 (G0), Synthesis (S), Gap 2 (G2) and Mitosis (M) are negotiated through a series of check points during which the favorability of the local cellular environment is assessed, prior to replicating DNA [1]. Cyclin D1 has been characterized as a key regulatory subunit of the holoenzyme that promotes the G1/S-phase transition through phosphorylating the pRB protein...
July 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28746211/epidermal-growth-factor-promotes-proliferation-and-maintains-multipotency-of-continuous-cultured-adipose-stem-cells-via-activating-stat-signal-pathway-in-vitro
#14
Guihai Ai, Xiaowen Shao, Meng Meng, Liwen Song, Jin Qiu, Yi Wu, Jianhong Zhou, Jiajing Cheng, Xiaowen Tong
This study aimed to investigate the effects of epidermal growth factor (EGF) on the proliferation and differentiation of adipose stem cells (ASC) during the repeated passaging and probe the underlying signal pathway. Results showed that the Ki67 positive rate remained at a high level, the number of ASCs in G0/G1 phase reduced significantly, but ASCs in G2/M phase and S phase increased markedly in ASCs treated with EGF when compared with ASCs without EGF treatment, indicating that EGF made more ASCs in proliferation phase...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28745317/reticulocalbin-2-enhances-hepatocellular-carcinoma-proliferation-via-modulating-the-egfr-erk-pathway
#15
D Ding, H Huang, W Jiang, W Yu, H Zhu, J Liu, H Saiyin, J Wu, H Huang, S Jiang, L Yu
Hepatocellular carcinoma (HCC) is a major health threat worldwide. Although the involvement of reticulocalbin-2 (RCN2) in cell differentiation has been reported, its function in oncogenesis is poorly understood. Here, we showed that RCN2 was upregulated in tumors compared with adjacent non-tumorous tissues in HCC patients and RCN2 expression clinically correlated with tumor size, disease recurrence and survival rate. Both knockdown and knockout of RCN2 significantly inhibited HCC cell proliferation by inducing G1/S transition arrest and downregulating cyclin D1 expression, while the proliferative ability was restored in knockout HCC cells with exogenously expressed RCN2...
July 24, 2017: Oncogene
https://www.readbyqxmd.com/read/28744706/the-evolution-of-a-g1-s-transcriptional-network-in-yeasts
#16
REVIEW
Adi Hendler, Edgar M Medina, Nicolas E Buchler, Robertus A M de Bruin, Amir Aharoni
The G1-to-S cell cycle transition is promoted by the periodic expression of a large set of genes. In Saccharomyces cerevisiae G1/S gene expression is regulated by two transcription factor (TF) complexes, the MBF and SBF, which bind to specific DNA sequences, the MCB and SCB, respectively. Despite extensive research little is known regarding the evolution of the G1/S transcription regulation including the co-evolution of the DNA binding domains with their respective DNA binding sequences. We have recently examined the co-evolution of the G1/S TF specificity through the systematic generation and examination of chimeric Mbp1/Swi4 TFs containing different orthologue DNA binding domains in S...
July 25, 2017: Current Genetics
https://www.readbyqxmd.com/read/28743911/knockdown-of-anion-exchanger-2-suppressed-the-growth-of-ovarian-cancer-cells-via-mtor-p70s6k1-signaling
#17
Li-Jun Zhang, Renquan Lu, Ya-Nan Song, Jian-Yong Zhu, Wei Xia, Miao Zhang, Zhi-Yi Shao, Yan Huang, Yuqi Zhou, Hongqin Zhang, Lin Guo, Meiqin Zhang, Hong Zhang
Anion exchanger 2 (AE2, encoded by SLC4A2) is a sodium-independent chloride/bicarbonate transporter and implicated in the regulation of intracellular pH and membrane potential. Previous studies have linked AE2 to the tumorigenesis of various cancers. Here, AE2 was identified as an up-regulated protein in ovarian cancer tissues compared to adjacent non-tumor lesions based on quantitative proteomics analysis. AE2 mRNA was also overexpressed in human ovarian cancer samples, and that AE2 overexpression correlated with the shortened survival time of ovarian cancer patients...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743112/microrna-302b-3p-suppresses-cell-proliferation-through-akt-pathway-by-targeting-igf-1r-in-human-gastric-cancer
#18
Bo Guo, Zhenghao Zhao, Zhen Wang, Qian Li, Xiaofei Wang, Wenjing Wang, Tusheng Song, Che Huang
BACKGROUND/AIMS: MiR-302b is a major microRNA found in human embryonic stem cells and induced pluripotent stem cells. However, its function in gastric cancer progression remains unclear. METHODS: Quantitative reverse transcription-PCR was performed to detect the expression levels of miR-302b-3p in gastric cancer tissues. MTT, colony formation, and flow cytometer analyses were conducted to explore the function of miR-302b-3p in MKN-45/SGC-7901 cells. A dual-luciferase reporter was used to validate the bioinformatics-predicted target gene of miR-302b-3p...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28739738/cyclophilin-a-is-overexpressed-in-hepatocellular-carcinoma-and-is-associated-with-the-cell-cycle
#19
Zhaohua Gong, Cheng Chi, Xiaojuan Huang, Hongjin Chu, Jiahui Wang, Fengcai DU, Lixin Jiang, Jian Chen
AIM: To investigate the expression of cyclophilin A (CypA) in human hepatocellular carcinoma (HCC) and explore the effects of CypA on the cell cycle in HCC. MATERIALS AND METHODS: CypA expression was assessed by immunohistochemistry in 48 cases of HCC tissues and paired adjacent tissues. CypA plasmid was transfected into HCC cells and the cell cycle was analyzed. RESULTS: Positivity for CypA was higher in HCC tissues than in adjacent tissues (79...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28738086/an-essential-ebv-latent-antigen-3c-binds-bcl6-for-targeted-degradation-and-cell-proliferation
#20
Yonggang Pei, Shuvomoy Banerjee, Hem Chandra Jha, Zhiguo Sun, Erle S Robertson
The latent EBV nuclear antigen 3C (EBNA3C) is required for transformation of primary human B lymphocytes. Most mature B-cell malignancies originate from malignant transformation of germinal center (GC) B-cells. The GC reaction appears to have a role in malignant transformation, in which a major player of the GC reaction is Bcl6, a key regulator of this process. We now demonstrate that EBNA3C contributes to B-cell transformation by targeted degradation of Bcl6. We show that EBNA3C can physically associate with Bcl6...
July 2017: PLoS Pathogens
keyword
keyword
64714
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"