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https://www.readbyqxmd.com/read/28527751/hand-strengthening-exercises-in-chronic-stroke-patients-dose-response-evaluation-using-electromyography
#1
Jonas Vinstrup, Joaquin Calatayud, Markus D Jakobsen, Emil Sundstrup, Jørgen R Jørgensen, Jose Casaña, Lars L Andersen
STUDY DESIGN: Cross-sectional. PURPOSE OF THE STUDY: This study evaluates finger flexion and extension strengthening exercises using elastic resistance in chronic stroke patients. METHODS: Eighteen stroke patients (mean age: 56.8 ± 7.6 years) with hemiparesis performed 3 consecutive repetitions of finger flexion and extension, using 3 different elastic resistance levels (easy, moderate, and hard). Surface electromyography was recorded from the flexor digitorum superficialis (FDS) and extensor digitorum (ED) muscles and normalized to the maximal electromyography of the non-paretic arm...
May 17, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/28527253/-prevalence-of-mycobacterium-tuberculosis-resistance-to-quinolones-and-injectables-in-colombia-2012-2013
#2
Claudia Llerena, Angie Zabaleta, Angélica Valbuena, Martha Murcia
INTRODUCTION: Tuberculosis is a health problem worldwide. The World Health Organization estimated 9.6 million new cases and 480,000 multirresistant cases for 2014. The assessment of resistance to quinolones and injectables was implemented only a few years ago, so its prevalence is not known. OBJECTIVE: To determine the prevalence of resistance to amikacin, capreomycin and ofloxacin in cases of tuberculosis resistant to isoniazid and/or rifampin during 2012-2013...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28526987/assessment-of-antibiotic-use-in-farm-animals-in-rwanda
#3
Rosine Manishimwe, Kizito Nishimwe, Lonzy Ojok
The irrational use of antibiotics in humans and animals is highly related to the emergence and increase of antibiotic-resistant bacteria worldwide. A cross-sectional survey aimed at evaluating the current level of practices regarding antibiotic use in farm animals in Rwanda was carried out countrywide. Interviews were conducted on 229 farmers rearing different types of animals. The study has revealed that almost all respondent farmers could name at least one antibiotic used in farm animals and peni-streptomycin was named by most of them (95...
May 19, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28526786/impact-of-acanthamoeba-cysts-on-stress-resistance-of-salmonella-enterica-serovar-typhimurium-yersinia-enterocolitica-4-o-3-listeria-monocytogenes-1-2a-and-escherichia-coli-o-26
#4
Ellen Lambrecht, Julie Baré, Koen Sabbe, Kurt Houf
The formation of robust resting cysts enables Acanthamoeba to resist harsh environmental conditions. This study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. Moreover it was assessed whether certain intracystic meatborne bacterial pathogens are more stress-resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cross-tolerance towards other stressors...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526748/the-sialate-o-acetylesterase-esta-from-gut-bacteroidetes-species-enables-sialidase-mediated-cross-species-foraging-of-9-o-acetylated-sialoglycans
#5
Lloyd S Robinson, Warren G Lewis, Amanda L Lewis
The gut harbors many symbiotic, commensal, and pathogenic microbes that engage in the breakdown and metabolism of host carbohydrates. Sialic acids are prominent outermost carbohydrates on mucins and protect underlying glycan chains from enzymatic degradation. Sialidases produced by some members of the colonic microbiota have been shown to promote the expansion of several potential pathogens (e.g. Clostridium difficile, Salmonella, Escherichia coli) that do not produce sialidases. O-acetyl ester modifications of sialic acids help resist the action of many sialidases and are found at high levels in the mammalian colon...
May 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28525391/titanium-dioxide-nanowire-sensor-array-integration-on-cmos-platform-using-deterministic-assembly
#6
Oren Gall, Xiahua Zhong, Daniel Schulman, Myungkoo Kang, Ali Razavieh, Theresa Mayer
Nanosensor arrays have recently received significant attention due to their utility in a wide range of applications, including gas sensing, fuel cells, internet of things (IoT), and portable health monitoring systems. Less attention has been given to the production of sensor platforms in the µW range for ultra-low power applications. Here, we discuss how to scale the nanosensor energy demand by developing a process for integration of nanowire sensing arrays on a monolithic CMOS chip. This work demonstrates an off-chip nanowire fabrication method; subsequently nanowires link to a fused SiO2 substrate using electric-field assisted directed assembly...
May 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28525328/establishment-of-5-fluorouracil-resistant-canine-mammary-tumor-cell-line
#7
B Zhou, D Zhang, S M Pei, H Zhang, H C Du, Y P Jin, D G Lin
Canine mammary tumors are the most common neoplasms in intact female dogs. The surgery cannot always solve the problem, chemotherapy are recommend to these patients. However, chemotherapy could always fail because of multidrug resistance (MDR). Through stepwise increasing 5-Fluorouracil (5-FU) concentration in the culture medium, a 5-FU-resistant canine mammary tumor cell line CMT7364/5-FU was established to disclose the molecular mechanism of the drug resistance. Cell morphology, cell sensitivity to drugs, growth curves, expression of proteins, and chemo-sensitivity in vivo were compared between the parental cell line and resistant cell line...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523846/a-novel-rapid-analysis-using-mass-spectrometry-to-evaluate-downstream-refolding-of-recombinant-human-insulin-like-growth-factor-1-mecasermin
#8
Kenichiro Furuki, Toshimasa Toyo'oka, Hideto Yamaguchi
RATIONALE: Mecasermin is used to treat elevated blood sugar as well as growth hormone-resistant Laron-type dwarfism. Mecasermin isolated from inclusion bodies in extracts of E.coli must be refolded to acquire sufficient activity. However, there is no rapid analytical method for monitoring refolding during the purification process. METHODS: We prepared mecasermin drug product, in-process samples during the oxidation of mecasermin, forced-reduced mecasermin, and aerially oxidized mecasermin after forced reduction...
May 19, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28523792/the-impact-of-calcineurin-inhibitors-on-neutrophil-gelatinase-associated-lipocalin-and-fibroblast-growth-factor-23-in-long-term-kidney-transplant-patients
#9
Inger Hjørdis Bleskestad, Inga Strand Thorsen, Grete Jonsson, Øyvind Skadberg, Lasse Gunnar Gøransson
BACKGROUND: Neutrophil gelatinase-associated lipocalin (NGAL), a protein with bacteriostatic functions rapidly excreted from stimulated or damaged epithelial cells, is elevated in acute and chronic kidney disease. A calcineurin dependent signaling pathway for fibroblast growth factor 23 (FGF23) has been revealed, but the effect of calcineurin inhibitors (CNIs) on the levels of NGAL and markers of mineral metabolism in long-term kidney transplant patients has not been explored. METHODS: In a cross-sectional study, 39 patients who received a first kidney transplant more than 10 years ago were split into two groups based on whether (n=28) or not (n=11) they used CNIs...
May 19, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28523469/system-for-automatic-heart-rate-calculation-in-epileptic-seizures
#10
Marcin Kołodziej, Andrzej Majkowski, Remigiusz J Rak, Bartosz Świderski, Andrzej Rysz
This article presents a comprehensive system for automatic heart rate (HR) detection. The system is robust and resistant to disturbances (noise, interferences, artifacts) occurring mainly during epileptic seizures. ECG signal filtration (IIR) and normalization due to skewness and standard deviation were used as preprocessing steps. A key element of the system is a reference QRS complex pattern calculated individually for each ECG recording. Next, a cross-correlation of the reference QRS pattern with short, normalized ECG windows is calculated and the maxima of the correlation are found (R-wave locations)...
May 18, 2017: Australasian Physical & Engineering Sciences in Medicine
https://www.readbyqxmd.com/read/28523017/antibiotic-susceptibility-in-commonly-isolated-pathogens-from-urinary-tract-infection-in-a-cohort-of-subjects-from-low-socioeconomic-strata
#11
Saera Suhail Kidwai, Ayesha Nageen, Samina Ghaznavi, Farhat Bashir, Jamal Ara
BACKGROUND AND OBJECTIVE: Urinary tract infection is one of the commonest infections seen in clinical practice. Lack of compliance and unjustified antibiotic prescriptions has resulted in bacterial resistance and is proving as a major challenge in the management of these infections. Our aim was to identify the sensitivity pattern of commonly used antibiotics against urinary tract infections so as to suggest an improvised line of action against bacteria causing urinary tract infections'...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28523008/genetic-interaction-between-arabidopsis-qpm3-1-locus-and-bacterial-effector-gene-hopw1-1-underlies-natural-variation-in-quantitative-disease-resistance-to-pseudomonas-infection
#12
Qi Luo, Wei-Wei Liu, Ke-Di Pan, You-Liang Peng, Jun Fan
Wide quantitative variation in plant disease resistance across Arabidopsis wild populations has been documented and the underlying mechanisms remain largely unknown. To investigate the genetic and molecular basis of this variation, Arabidopsis recombinant inbred lines (RILs) derived from Aa-0 × Col-0 and Gie-0 × Col-0 crosses were constructed and used for inoculation with Pseudomonas syringae pathovars maculicola ES4326 (ES4326) and tomato DC3000 (DC3000). Bacterial growth assays revealed continuous distribution across the large differences between the most and the least susceptible lines in the RILs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28521955/prevalence-of-relevant-ns5a-resistance-associated-substitutions-to-elbasvir-in-genotype-1a-hepatitis-c-virus-patients-in-spain
#13
Claudia Palladino, Beatriz Esteban-Cartelle, Irene Mate-Cano, Marta Sánchez-Carrillo, Salvador Resino, Verónica Briz
Resistance-associated substitutions (RASs) to the new HCV NS5A inhibitor elbasvir may limit its efficacy and lead to virological failure in HCV-GT1a-infected patients. There are no data outside clinical trials evaluating their prevalence and impact in grazoprevir/elbasvir in GT1a-infected patients in Spain. A multicentre cross-sectional study of 632 initial patients was conducted. In 13 of these patients, the sample could not be amplified or a consensus sequence by Sanger sequencing could not be performed. Ultimately, 617 HCV-G1a-infected individuals treated at 84 Spanish hospitals from the 17 autonomous communities plus the 2 autonomous cities of Spain were analysed...
May 15, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28521719/virological-suppression-and-patterns-of-resistance-amongst-patients-on-antiretroviral-therapy-at-4-nigerian-military-hospitals
#14
Keshinro Babajide, Ayemoba Ojor, Terfa Kene, Ake Julie, Crowell Trevor A, Adamu Yakubu, Mohammed Tahir, Okoye Ifeanyi, Odeyemi Sunday, Crawford Keith, Hughes Lindsay, Akintunde Ezekiel, Umar Tahir, Hamm Tiffany E, Njoku Ogbonnaya S
BACKGROUND: In resource-constrained settings, plasma HIV-1 RNA quantification has not been routinely available for the monitoring of response to antiretroviral therapy. This study evaluated virological suppression rates amongst patients on first-line ART in four Nigerian military hospitals. METHODS: We conducted a cross-sectional study of 325 randomly selected adult clinic clients (≥18 years old) on first-line ART regimens at four Nigerian military hospitals. Plasma HIV-1 RNA was assayed using a Roche COBAS TaqMan48 with High Pure System...
May 16, 2017: Current HIV Research
https://www.readbyqxmd.com/read/28521110/ethanol-induced-stress-response-of-staphylococcus-aureus
#15
Jasmine M Pando, Richard F Pfeltz, Jesus A Cuaron, Vijayaraj Nagarajan, Mukti N Mishra, Nathanial J Torres, Mohamed O Elasri, Brian J Wilkinson, John E Gustafson
Transcriptional profiles of two unrelated clinical methicillin-resistant S. aureus (MRSA) isolates were analyzed following 10 % v/v ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common to both strains, of which 291 were up-regulated. With the exception of the down-regulation of genes involved with osmotic stress functions, EIS resulted in the up-regulation of genes that contribute to stress response networks, notably those altered by oxidative stress, protein quality control in general, and heat shock in particular...
May 18, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28518147/survivin-a-key-player-in-cancer-progression-increases-in-obesity-and-protects-adipose-tissue-stem-cells-from-apoptosis
#16
Miriam Ejarque, Victòria Ceperuelo-Mallafré, Carolina Serena, Gisela Pachón, Yaiza Núñez-Álvarez, Margarida Terrón-Puig, Enrique Calvo, Catalina Núñez-Roa, Wilfredo Oliva-Olivera, Francisco J Tinahones, Miguel Angel Peinado, Joan Vendrell, Sonia Fernández-Veledo
Adipose tissue (AT) has a central role in obesity-related metabolic imbalance through the dysregulated production of cytokines and adipokines. In addition to its known risk for cardiovascular disease and diabetes, obesity is also a major risk for cancer. We investigated the impact of obesity for the expression of survivin, an antiapoptotic protein upregulated by adipokines and a diagnostic biomarker of tumor onset and recurrence. In a cross-sectional study of 111 subjects classified by body mass index, circulating levels of survivin and gene expression in subcutaneous AT were significantly higher in obese patients and positively correlated with leptin...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28518103/structure-function-studies-in-mouse-embryonic-stem-cells-using-recombinase-mediated-cassette-exchange
#17
Tim Pieters, Lieven Haenebalcke, Kenneth Bruneel, Niels Vandamme, Tino Hochepied, Jolanda van Hengel, Dagmar Wirth, Geert Berx, Jody J Haigh, Frans van Roy, Steven Goossens
Gene engineering in mouse embryos or embryonic stem cells (mESCs) allows for the study of the function of a given protein. Proteins are the workhorses of the cell and often consist of multiple functional domains, which can be influenced by posttranslational modifications. The depletion of the entire protein in conditional or constitutive knock-out (KO) mice does not take into account this functional diversity and regulation. An mESC line and a derived mouse model, in which a docking site for FLPe recombination-mediated cassette exchange (RMCE) was inserted within the ROSA26 (R26) locus, was previously reported...
April 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516806/is-bisphenol-a-exposure-associated-with-the-development-of-glucose-intolerance-and-increased-insulin-resistance-in-thais
#18
La-Or Chailurkit, Pechngam Tengpraettanakorn, Suwannee Chanprasertyotin, Boonsong Ongphiphadhanakul
Bisphenol A (BPA), the monomeric component of polycarbonate plastics, reportedly possesses endocrine-disrupting effects. Exposure to low levels of BPA during more vulnerable periods leads to abnormalities related to sexual development in experimental animals. Moreover, recently a few epidemiological studies in Caucasians have demonstrated the association of BPA exposure with type 2 diabetes. Therefore, in the present study we examined the association of BPA exposure and abnormal glucose tolerance in Thais. This is a cross-sectional study of 240 participants aged at least 50 years, randomly selected by computer-generated random numbers within each glucose tolerance status from an oral glucose tolerance study of 661 participants...
January 1, 2017: Nutrition and Health
https://www.readbyqxmd.com/read/28515978/variation-in-medial-and-lateral-gastrocnemius-muscle-activity-with-foot-position
#19
Michael Cibulka, April Wenthe, Zach Boyle, Dylan Callier, Adam Schwerdt, Deidra Jarman, Michael J Strube
BACKGROUND: The gastrocnemius has two heads, medial gastrocnemius (MG) and lateral gastrocnemius (LG); little is known how they contract with different foot positions. The MG is more frequently strained than the LG; and gastrocnemius activation pattern altered by foot position may play a role in injury. Leg exercises often use a toe-in versus toe-out foot position to isolate one gastrocnemius head over another. PURPOSE: The purpose of this study was to determine the electromyographic gastrocnemius muscle activity in the toe-out and toe-in foot positions during weight bearing and non-weight bearing activities...
April 2017: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/28515905/colonization-of-long-term-care-facility-patients-with-mdr-gram-negatives-during-an-acinetobacter-baumannii-outbreak
#20
Ines Zollner-Schwetz, Elisabeth Zechner, Elisabeth Ullrich, Josefa Luxner, Christian Pux, Gerald Pichler, Walter Schippinger, Robert Krause, Eva Leitner
BACKGROUND: We aimed to determine the prevalence of colonization by multidrug-resistant Gram-negative bacteria including ESBL-producing enterobacteriaceae, carbapenem-resistant enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii at two wards caring long term for patients with disorder of consciousness at the Geriatric Health Centers Graz, Austria. During our study we detected two A. baumannii outbreaks. METHODS: In August 2015, we conducted a point-prevalence study...
2017: Antimicrobial Resistance and Infection Control
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