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https://www.readbyqxmd.com/read/27914475/an-observational-study-of-febrile-seizures-the-importance-of-viral-infection-and-immunization
#1
Joshua R Francis, Peter Richmond, Christine Robins, Katie Lindsay, Avram Levy, Paul V Effler, Meredith Borland, Christopher C Blyth
BACKGROUND: Febrile seizures are common in young children. Annual peaks in incidence mirror increased respiratory virus activity during winter. Limited virological data are available using modern diagnostic techniques for children with febrile seizures. We aimed to determine the frequency of detection of specific viral pathogens in children with febrile seizures, to describe risk factors including recent vaccination and clinical features associated with specific etiologies. METHODS: An observational study was performed...
December 3, 2016: BMC Pediatrics
https://www.readbyqxmd.com/read/27852673/progress-in-quantitative-viral-load-testing-variability-and-impact-of-the-who-quantitative-international-standards
#2
R T Hayden, Y Sun, L Tang, G W Procop, D R Hillyard, B A Pinsky, S A Young, A M Caliendo
It has been hoped that recent availability of WHO quantitative standards would improve inter-laboratory agreement for viral load testing; however, insufficient data are available to evaluate whether this has been the case. Results from 554 laboratories participating in proficiency testing surveys for quantitative CMV, EBV, BKV, ADV and HHV6 PCR testing were evaluated to determine result variability overall, and then stratified by assay manufacturer. The impact of calibration to IU/mL (CMV and EBV) on variability was also determined...
November 16, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27830722/comprehensive-polymerase-chain-reaction-assay-for-detection-of-pathogenic-dna-in-lymphoproliferative-disorders-of-the-ocular-adnexa
#3
Yoshihiko Usui, Narsing A Rao, Hiroshi Takase, Kinya Tsubota, Kazuhiko Umazume, Daniel Diaz-Aguilar, Takeshi Kezuka, Manabu Mochizuki, Hiroshi Goto, Sunao Sugita
Infectious agents have been identified as a major cause of specific types of human cancers worldwide. Several microorganisms have been identified as potential aggravators of ocular adnexal neoplasms; however, given the rarity of these neoplasms, large epidemiological studies are difficult to coordinate. This study aimed to conduct an exhaustive search for pathogenic DNA in lymphoproliferative disorders (LPD) of the ocular adnexa in a total of 70 patients who were diagnosed with LPD of the ocular adnexa between 2008 and 2013...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27800078/-molecular-tests-in-diagnosis-of-cytomegalovirus-cmv-human-herpesvirus-6-hhv-6-and-epstein-barr-virus-ebv-using-real-time-pcr-in-hiv-positive-and-hiv-negative-pregnant-women-in-ouagadougou-burkina-faso
#4
Alice Rogomenoma Ouedraogo, Madeleine Kabre, Cyrille Bisseye, Théodora Mahoukèdè Zohoncon, Maleki Asshi, Serge Théophile Soubeiga, Birama Diarra, Lassina Traore, Florencia Wendkuuni Djigma, Djénéba Ouermi, Virginio Pietra, Nicolas Barro, Jacques Simpore
INTRODUCTION: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou. METHODS: In this study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected three infections (EBV, CMV and HHV-6)...
2016: Pan African Medical Journal
https://www.readbyqxmd.com/read/27794237/cognitive-functioning-one-month-and-one-year-following-febrile-status-epilepticus
#5
Erica F Weiss, David Masur, Shlomo Shinnar, Dale C Hesdorffer, Veronica J Hinton, Melanie Bonner, Julie Rinaldi, Virginia Van de Water, James Culbert, Ruth C Shinnar, Syndi Seinfeld, William Gallentine, Douglas R Nordli, L Mathew Frank, Leon Epstein, Solomon L Moshé, Shumei Sun
OBJECTIVE: The objective of this study was to determine early developmental and cognitive outcomes of children with febrile status epilepticus (FSE) one month and one year after FSE. METHODS: One hundred ninety four children with FSE were evaluated on measures of cognition, receptive language, and memory as part of the FEBSTAT study and compared with 100 controls with simple febrile seizures (FSs). RESULTS: Children with FSE did not differ dramatically on tasks compared with FS controls at one month after FSE but demonstrated slightly weaker motor development (p=0...
November 2016: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/27697585/human-herpesvirus-6-infection-following-haploidentical-transplantation-immune-recovery-and-outcome
#6
Raffaella Greco, Lara Crucitti, Maddalena Noviello, Sara Racca, Daniele Mannina, Alessandra Forcina, Francesca Lorentino, Veronica Valtolina, Serena Rolla, Roee Dvir, Mara Morelli, Fabio Giglio, Maria Chiara Barbanti, Maria Teresa Lupo Stanghellini, Chiara Oltolini, Luca Vago, Paolo Scarpellini, Andrea Assanelli, Matteo G Carrabba, Sarah Marktel, Massimo Bernardi, Consuelo Corti, Massimo Clementi, Jacopo Peccatori, Chiara Bonini, Fabio Ciceri
Human herpesvirus 6 (HHV-6) is increasingly recognized as a potentially life-threatening pathogen in allogeneic hematopoietic stem cell transplantation (alloSCT). We retrospectively evaluated 54 adult patients who developed positivity to HHV-6 after alloSCT. The median time from alloSCT to HHV-6 reactivation was 34 days. HHV-6 was present in plasma samples from 31 patients, in bone marrow (BM) of 9 patients, in bronchoalveolar lavage fluid and liver or gut biopsy specimens from 33 patients, and in cerebrospinal fluid of 7 patients...
September 30, 2016: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/27367597/presence-of-hhv-6a-in-endometrial-epithelial-cells-from-women-with-primary-unexplained-infertility
#7
Roberto Marci, Valentina Gentili, Daria Bortolotti, Giuseppe Lo Monte, Elisabetta Caselli, Silvia Bolzani, Antonella Rotola, Dario Di Luca, Roberta Rizzo
To elucidate the roles of human herpesvirus (HHV)-6 primary unexplained infertile women, a prospective randomized study was conducted on a cohort of primary unexplained infertile women and a cohort of control women, with at least one successful pregnancy. HHV-6 DNA was analyzed and the percentage and immune-phenotype of resident endometrial Natural Killer (NK) cells, as the first line of defense towards viral infections, was evaluated in endometrial biopsies. Cytokine levels in uterine flushing samples were analyzed...
2016: PloS One
https://www.readbyqxmd.com/read/27364410/virus-reactivations-after-autologous-hematopoietic-stem-cell-transplantation-detected-by-multiplex-pcr-assay
#8
Natsuko Inazawa, Tsukasa Hori, Masanori Nojima, Makoto Saito, Keita Igarashi, Masaki Yamamoto, Norio Shimizu, Yuko Yoto, Hiroyuki Tsutsumi
Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple viral reactivations simultaneously in 24 patients undergoing auto-HSCT between September 2010 and December 2012. Weekly whole blood samples were collected from pre- to 42 days post-HSCT, and tested for the following 13 viruses; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6), HHV-7, HHV-8, adeno virus (ADV), BK virus (BKV), JC virus (JCV), parvovirus B19 (B19V), and hepatitis B virus (HBV)...
February 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/27339696/divergent-viral-presentation-among-human-tumors-and-adjacent-normal-tissues
#9
Song Cao, Michael C Wendl, Matthew A Wyczalkowski, Kristine Wylie, Kai Ye, Reyka Jayasinghe, Mingchao Xie, Song Wu, Beifang Niu, Robert Grubb, Kimberly J Johnson, Hiram Gay, Ken Chen, Janet S Rader, John F Dipersio, Feng Chen, Li Ding
We applied a newly developed bioinformatics system called VirusScan to investigate the viral basis of 6,813 human tumors and 559 adjacent normal samples across 23 cancer types and identified 505 virus positive samples with distinctive, organ system- and cancer type-specific distributions. We found that herpes viruses (e.g., subtypes HHV4, HHV5, and HHV6) that are highly prevalent across cancers of the digestive tract showed significantly higher abundances in tumor versus adjacent normal samples, supporting their association with these cancers...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27272972/hv-6-and-seizure-a-systematic-review-and-meta-analysis
#10
Fatemeh Mohammadpour Touserkani, Marina Gaínza Lein, Saba Jafarpour, Katelyn Brinegar, Kush Kapur, Tobias Loddenkemper
OBJECTIVES: Human herpesvirus-6 (HHV-6) is a common infection in childhood. This systematic review aims to assess the relationship between HHV-6 infection and seizure disorders based on available literature. METHODS: MEDLINE database was searched using a search strategy. We included studies that were published after 1993 in English, and described patients with HHV-6 infection and accompanying seizure or status epilepticus. RESULTS: We identified 503 articles, and included 147 studies...
June 8, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27270692/acute-onset-myopericarditis-as-unusual-presentation-of-primary-hiv-infection
#11
Giacomo Vandi, Leonardo Calza, Nicolò Girometti, Roberto Manfredi, Giuseppina Musumeci, Isabella Bon, Maria Carla Re
A 30-year-old man was admitted to hospital after complaining of a retrosternal burning pain, radiating to the jugular region, and to both upper limbs. An electrocardiography examination showed a ST segment elevation involving the lower-lateral leads. A trans-thoracic ultrasonography showed findings compatible with an acute myopericarditis. All performed serological testings excluded other recent infections with cardiac tropism. Among screening tests, a peripheral lymphocyte subset analysis was performed and an inversion of the CD4/CD8 ratio was found...
June 6, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/26664436/human-herpes-virus-type-6-and-febrile-convulsion
#12
Mohammad Mehdi Houshmandi, Alireza Moayedi, Mohammad Bagher Rahmati, Abdulmajid Nazemi, Darioush Fakhrai, Shahram Zare
OBJECTIVE: Febrile Convulsion (FC) is occurred in 6 months to 5 yr old children. The aim of this study was to investigate the prevalence of HHV-6 infection in FC admitted patients of Bandar Abbas Children Hospital, southern Iran. MATERIALS & METHODS: In a cross-sectional study, 118 children aged 6-60 months who had FC were selected by a simple random method in 2010-11. Demographic data, clinical manifestation and two blood samples gathered to assess the human herpes virus type 6 (HHV6)...
2015: Iranian Journal of Child Neurology
https://www.readbyqxmd.com/read/26640344/evaluation-of-a-multiplex-pcr-assay-for-detection-of-cytomegalovirus-in-stool-samples-from-patients-with-ulcerative-colitis
#13
Saifun Nahar, Atsushi Iraha, Akira Hokama, Ayako Uehara, Gretchen Parrott, Tetsuya Ohira, Masatoshi Kaida, Tetsu Kinjo, Takeshi Kinjo, Tetsuo Hirata, Nagisa Kinjo, Jiro Fujita
AIM: To evaluate a multiplex PCR assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis (UC). METHODS: We prospectively analyzed 300 individuals, including immunocompetent patients, immunocompromised patients, and patients with UC. Stool samples were collected from the recto-sigmoid region of the colon by endoscopy. The samples were qualitatively analyzed for bacterial and viral enteropathogens with a multiplex PCR assay using a Seeplex(®) Kit...
November 28, 2015: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/26555946/simultaneous-quantification-of-the-8-human-herpesviruses-in-allogeneic-hematopoietic-stem-cell-transplantation
#14
Paulo Guilherme Alvarenga Gomes de Oliveira, Miriam Yurika Hiramoto Ueda, Juliana Monte Real, Eloisa de Sá Moreira, José Salvador Rodrigues de Oliveira, Matheus Vescovi Gonçalves, Valeria Cortez Ginani, Olga Margareth Wanderley de Oliveira Felix, Adriana Seber, Yana Novis, Vanderson Rocha, Celso Francisco Hernandes Granato, Celso Arrais-Rodrigues
BACKGROUND: Human herpesviruses may cause severe complications after allogeneic hematopoietic stem cell transplantation (HSCT). However, the impact of some of these infections on transplant outcomes is still unclear. A prospective survey on the incidence and clinical features of herpesviruses infections after HSCT has not yet been conducted in Brazilian patients, and the impact of these infections on HSCT outcome remains unclear. METHODS: We prospectively analyzed the incidence of infection of the eight human herpesviruses simultaneously in 1 045 peripheral blood samples from 98 allogeneic HSCT recipients...
June 2016: Transplantation
https://www.readbyqxmd.com/read/26493843/increased-risk-of-infections-and-infection-related-mortality-in-children-undergoing-haematopoietic-stem-cell-transplantation-compared-to-conventional-anticancer-therapy-a-multicentre-nationwide-study
#15
MULTICENTER STUDY
J Styczynski, K Czyzewski, M Wysocki, O Gryniewicz-Kwiatkowska, A Kolodziejczyk-Gietka, M Salamonowicz, L Hutnik, O Zajac-Spychala, A Zaucha-Prazmo, L Chelmecka-Wiktorczyk, K Siewiera, J Fraczkiewicz, Z Malas, R Tomaszewska, N Irga-Jaworska, M Plonowski, T Ociepa, F Pierlejewski, Z Gamrot, A Urbanek-Dadela, J Gozdzik, W Stolpa, B Dembowska-Baginska, D Perek, M Matysiak, J Wachowiak, J Kowalczyk, W Balwierz, K Kalwak, A Chybicka, W Badowska, T Szczepanski, E Drozynska, M Krawczuk-Rybak, T Urasinski, W Mlynarski, M Woszczyk, G Karolczyk, G Sobol-Milejska, L Gil
This nationwide multicentre study analysed the epidemiology of bacterial, viral and fungal infections in paediatric haematopoietic stem cell transplantation (HSCT) and paediatric haematology and oncology (PHO) patients over a period of 24 consecutive months, including incidence, hazard risk and outcome of infections as well as occurrence of multidrug-resistant bacteria. During this period, 308 HSCTs were performed and 1768 children were newly diagnosed for malignancy. Compared to PHO, the risk in HSCT patients was significantly higher for all infections (hazard ratio (HR) 2...
February 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/26081141/the-putative-role-of-viruses-bacteria-and-chronic-fungal-biotoxin-exposure-in-the-genesis-of-intractable-fatigue-accompanied-by-cognitive-and-physical-disability
#16
REVIEW
Gerwyn Morris, Michael Berk, Ken Walder, Michael Maes
Patients who present with severe intractable apparently idiopathic fatigue accompanied by profound physical and or cognitive disability present a significant therapeutic challenge. The effect of psychological counseling is limited, with significant but very slight improvements in psychometric measures of fatigue and disability but no improvement on scientific measures of physical impairment compared to controls. Similarly, exercise regimes either produce significant, but practically unimportant, benefit or provoke symptom exacerbation...
May 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/26078744/herpes-viral-origin-of-the-parsonage-turner-syndrome-highlighting-of-serological-immune-anti-herpes-deficiency-cured-by-anti-herpes-therapy
#17
Jacqueline Le Goaster, Patrice Bourée, Charles Ifergan, Frederic Tangy, René Olivier, Anne-Lise Haenni
In 2012, a 50 year-old athletic male presented with weakness, pain and unilateral phrenic paralysis, followed by bilateral phrenic paralysis with deep dyspnea. In 2013, the Parsonage-Turner syndrome was diagnosed. When the patient was seen in September 2014 for the first time, he was facing phrenic neuromuscular failure, which led to the hypothesis of neurotropic herpes viruses. A control of the global serological anti-Herpes immunity to analyze his antibody (Ab) levels confirmed herpes immune genetic deficiency...
May 2015: Case Reports in Neurology
https://www.readbyqxmd.com/read/26046087/broad-spectrum-antiviral-t-cells-for-viral-complications-after-hematopoietic-stem-cell-transplantation
#18
Britta Maecker-Kolhoff, Britta Eiz-Vesper
Major complications of hematopoietic stem cell transplantation (HSCT) or solid organ transplantation (SOT), such as graft rejection and graft-versus-host-disease (GvHD), are countered by suppressing the host immune system via chemotherapy and radiation, immunosuppressive drugs, or conditioning regimens such as in vivo or in vitro T-cell depletion. While immunocompromised, the patient is rendered susceptible to a number of viral infections and reactivations mainly caused by endogenous herpes viruses like cytomegalovirus (CMV) and Epstein-Barr virus (EBV) and by lytic agents such as adenovirus (ADV)...
May 2015: Annals of Translational Medicine
https://www.readbyqxmd.com/read/25939660/antivirus-immune-activity-in-multiple-sclerosis-correlates-with-mri-activity
#19
L B Latham, M J Lee, J A Lincoln, N Ji, T G Forsthuber, J W Lindsey
OBJECTIVE: The objective of this study was to determine whether reactivation of Epstein-Barr (EBV) or activation of the anti-EBV immune response correlates with MS disease activity on MR imaging. METHODS: Subjects with early, active relapsing-remitting MS were studied for 16 weeks with blood and saliva samples collected every 2 weeks and brain MRI performed every 4 weeks. We isolated peripheral blood mononuclear cells from each blood sample and tested the immune response to EBV, autologous EBV-infected lymphoblastoid cell lines (LCL), human herpesvirus 6 (HHV6), varicella zoster virus (VZV), tetanus, and mitogens...
January 2016: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/25807050/anti-cmv-igg-positivity-of-donors-is-beneficial-for-allohsct-recipients-with-respect-to-the-better-short-term-immunological-recovery-and-high-level-of-cd4-cd25high-lymphocytes
#20
Emilia Jaskula, Dorota Dlubek, Agnieszka Tarnowska, Janusz Lange, Monika Mordak-Domagala, Krzysztof Suchnicki, Mariola Sedzimirska, Agata Borowik, Sylwia Mizia, Andrzej Lange
Hematopoietic stem cell transplantation from anti-cytomegalovirus immunoglobulin G (anti-CMV-IgG) positive donors facilitated immunological recovery post-transplant, which may indicate that chronic CMV infection has an effect on the immune system. This can be seen in the recipients after reconstitution with donor lymphocytes. We evaluated the composition of lymphocytes at hematologic recovery in 99 patients with hematologic malignancies post hematopoietic stem cell transplantation (HSCT). Anti-CMV-IgG seropositivity of the donor was associated with higher proportions of CD4+ (227...
March 2015: Viruses
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