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Chaperonine

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https://www.readbyqxmd.com/read/28906489/disrupting-cct-%C3%AE-%C3%A2-%C3%A2-%C3%AE-tubulin-selectively-kills-cct-%C3%AE-overexpressed-cancer-cells-through-mapks-activation
#1
Yan-Jin Liu, Vathan Kumar, Yuan-Feng Lin, Po-Huang Liang
We have previously demonstrated the ability of I-Trp to disrupt the protein-protein interaction of β-tubulin with chaperonin-containing TCP-1β (CCT-β). This caused more severe apoptosis in multidrug-resistant MES-SA/Dx5, compared to MES-SA, due to its higher CCT-β overexpression. In this study, we screened a panel of cancer cell lines, finding CCT-β overexpression in the triple-negative breast cancer cell line MDA-MB-231, colorectal cancer cell lines Colo205 and HCT116, and a gastric cancer cell line MKN-45...
September 14, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28888227/conversion-of-an-inactive-xylose-isomerase-into-a-functional-enzyme-by-co-expression-of-groel-groes-chaperonins-in-saccharomyces-cerevisiae
#2
Beatriz Temer, Leandro Vieira Dos Santos, Victor Augusti Negri, Juliana Pimentel Galhardo, Pedro Henrique Mello Magalhães, Juliana José, Cidnei Marschalk, Thamy Lívia Ribeiro Corrêa, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira
BACKGROUND: Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel. Though xylose isomerase (XI) is the key enzyme for xylose conversion, almost half of the XI genes are not functional when expressed in S. cerevisiae. To date, protein misfolding is the most plausible hypothesis to explain this phenomenon...
September 9, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28883688/a-controversial-medicolegal-issue-timing-the-onset-of-perinatal-hypoxic-ischemic-brain-injury
#3
REVIEW
Vittorio Fineschi, Rocco Valerio Viola, Raffaele La Russa, Alessandro Santurro, Paola Frati
Perinatal hypoxic-ischemic brain injury, as a result of chronic, subacute, and acute insults, represents the pathological consequence of fetal distress and birth or perinatal asphyxia, that is, "nonreassuring fetal status." Hypoxic-ischemic injury (HII) is typically characterized by an early phase of damage, followed by a delayed inflammatory local response, in an apoptosis-necrosis continuum. In the early phase, the cytotoxic edema and eventual acute lysis take place; with reperfusion, additional damage should be assigned to excitotoxicity and oxidative stress...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28881650/apex2-enhanced-electron-microscopy-distinguishes-sigma-1-receptor-localization-in-the-nucleoplasmic-reticulum
#4
Timur A Mavlyutov, Huan Yang, Miles L Epstein, Arnold E Ruoho, Jay Yang, Lian-Wang Guo
The sigma-1 receptor (Sig1R) is an endoplasmic reticulum chaperonin that is attracting tremendous interest as a potential anti-neurodegenerative target. While this membrane protein is known to reside in the inner nuclear envelope (NE) and influences transcription, apparent Sig1R presence in the nucleoplasm is often observed, seemingly contradicting its NE localization. We addressed this confounding issue by applying an antibody-free approach of electron microscopy (EM) to define Sig1R nuclear localization. We expressed APEX2 peroxidase fused to Sig1R-GFP in a Sig1R-null NSC34 neuronal cell line generated with CRISPR-Cas9...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28880864/identification-of-an-allosteric-network-that-influences-assembly-and-function-of-group-ii-chaperonins
#5
Mingliang Jin, Yao Cong
No abstract text is available yet for this article.
September 7, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28861940/mapping-the-spatial-proteome-of-metastatic-cells-in-colorectal-cancer
#6
Marta Mendes, Alberto Peláez-García, María López-Lucendo, Rubén A Bartolomé, Eva Calviño, Rodrigo Barderas, J Ignacio Casal
Colorectal cancer (CRC) is the second deadliest cancer worldwide. Here, we aimed to study metastasis mechanisms using spatial proteomics in the KM12 cell model. Cells were SILAC-labeled and fractionated into five subcellular fractions corresponding to: cytoplasm, plasma, mitochondria and ER/golgi membranes, nuclear, chromatin-bound and cytoskeletal proteins and analyzed with high resolution mass spectrometry. We provide localization data of 4863 quantified proteins in the different subcellular fractions. A total of 1318 proteins with at least 1...
August 31, 2017: Proteomics
https://www.readbyqxmd.com/read/28852778/pathogenic-mutation-in-the-als-ftd-gene-ccnf-causes-elevated-lys48-linked-ubiquitylation-and-defective-autophagy
#7
Albert Lee, Stephanie L Rayner, Serene S L Gwee, Alana De Luca, Hamideh Shahheydari, Vinod Sundaramoorthy, Audrey Ragagnin, Marco Morsch, Rowan Radford, Jasmin Galper, Sarah Freckleton, Bingyang Shi, Adam K Walker, Emily K Don, Nicholas J Cole, Shu Yang, Kelly L Williams, Justin J Yerbury, Ian P Blair, Julie D Atkin, Mark P Molloy, Roger S Chung
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are fatal neurodegenerative disorders that have common molecular and pathogenic characteristics, such as aberrant accumulation and ubiquitylation of TDP-43; however, the mechanisms that drive this process remain poorly understood. We have recently identified CCNF mutations in familial and sporadic ALS and FTD patients. CCNF encodes cyclin F, a component of an E3 ubiquitin-protein ligase (SCF(cyclin F)) complex that is responsible for ubiquitylating proteins for degradation by the ubiquitin-proteasome system...
August 29, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28852160/creating-the-functional-single-ring-groel-groes-chaperonin-systems-via-modulating-groel-groes-interaction
#8
Melissa Illingworth, Holly Ellis, Lingling Chen
Chaperonin and cochaperonin, represented by E. coli GroEL and GroES, are essential molecular chaperones for protein folding. The double-ring assembly of GroEL is required to function with GroES, and a single-ring GroEL variant GroEL(SR) forms a stable complex with GroES, arresting the chaperoning reaction cycle. GroES I25 interacts with GroEL; however, mutations of I25 abolish GroES-GroEL interaction due to the seven-fold mutational amplification in heptameric GroES. To weaken GroEL(SR)-GroES interaction in a controlled manner, we used groES (7), a gene linking seven copies of groES, to incorporate I25 mutations in selected GroES modules in GroES(7)...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815858/loss-of-chloroplast-localized-protein-phosphatase-2cs-in-arabidopsis-thaliana-leads-to-enhancement-of-the-plant-immunity-and-resistance-to-xanthomonas-campestris-pv-campestris-infection
#9
Chiharu Akimoto-Tomiyama, Shigeru Tanabe, Hideyuki Kajiwara, Eiichi Minami, Hirokazu Ochiai
Protein phosphatases (PPs) counteract kinases in reversible phosphorylation events during numerous signal transduction pathways in eukaryotes. PP2Cs, one of the four major classes of serine/threonine specific PPs family is greatly expanded in plants. Thus PP2C is thought to have a specific role in a signal transduction pathway. Some rice PP2Cs classified to subgroup K were responsive to the infection by the compatible Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight. In A. thaliana, orthologous PP2C genes (AtPP2C62 and AtPP2C26) classified to subgroup K were also responsive to Xanthomonas campestris pv...
August 16, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28774748/investigation-on-different-chemical-stability-of-mitochondrial-hsp60-and-its-precursor
#10
Caterina Ricci, Rita Carrotta, Giacoma Cinzia Rappa, Maria Rosalia Mangione, Fabio Librizzi, Pier Luigi San Biagio, Heinz Amenitsch, Maria Grazia Ortore, Silvia Vilasi
In the large class of molecules that maintain protein homeostasis, called molecular chaperones, chaperonins constitute a subclass that specifically assist the correct folding of newly synthesized proteins. Among them, Hsp60 is composed of a double heptameric ring structure with a large central cavity where the unfolded protein binds via hydrophobic interactions and is supported, in this function, by the co-chaperonin Hsp10. Hsp60 is typically located in the mitochondria, but in some pathological situations, such as cancers and chronic inflammatory diseases, Hsp60 accumulates in the cytoplasm...
July 26, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28767434/chaperonin-containing-tcp1-subunit-5-is-a-tumor-associated-antigen-of-non-small-cell-lung-cancer
#11
Hongjun Gao, Min Zheng, Sijin Sun, Hongwu Wang, Zhigang Yue, Yun Zhu, Xiaochen Han, Junquan Yang, Yanqiu Zhou, Yiran Cai, Wanning Hu
Novel tumor antigens and their related autoantibodies have tremendous potential for early diagnosis of non-small cell lung cancer (NSCLC). In this study, we identify antigens from NSCLC tissue and autoantibodies in sera of patients with NSCLC using a modified proteomics-based approach. We seperated and identified four NSCLC-associated proteins extracted from the cytosol in tumor tissues by mini-two-dimensional gel electrophoresis, followed by Western blot and hybridization with individual sera for confirmation of antibody binding...
July 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28744329/psma-targeted-theranostic-nanocarrier-for-prostate-cancer
#12
Orielyz Flores, Santimukul Santra, Charalambos Kaittanis, Rania Bassiouni, Amr S Khaled, Annette R Khaled, Jan Grimm, J Manuel Perez
Herein, we report the use of a theranostic nanocarrier (Folate-HBPE(CT20p)) to deliver a therapeutic peptide to prostate cancer tumors that express PSMA (folate hydrolase 1). The therapeutic peptide (CT20p) targets and inhibits the chaperonin-containing TCP-1 (CCT) protein-folding complex, is selectively cytotoxic to cancer cells, and is non-toxic to normal tissue. With the delivery of CT20p to prostate cancer cells via PSMA, a dual level of cancer specificity is achieved: (1) selective targeting to PSMA-expressing prostate tumors, and (2) specific cytotoxicity to cancer cells with minimal toxicity to normal cells...
2017: Theranostics
https://www.readbyqxmd.com/read/28741612/an-information-theoretic-framework-reveals-a-tunable-allosteric-network-in-group-ii-chaperonins
#13
Tom Lopez, Kevin Dalton, Anthony Tomlinson, Vijay Pande, Judith Frydman
ATP-dependent allosteric regulation of the ring-shaped group II chaperonins remains ill defined, in part because their complex oligomeric topology has limited the success of structural techniques in suggesting allosteric determinants. Further, their high sequence conservation has hindered the prediction of allosteric networks using mathematical covariation approaches. Here, we develop an information theoretic strategy that is robust to residue conservation and apply it to group II chaperonins. We identify a contiguous network of covarying residues that connects all nucleotide-binding pockets within each chaperonin ring...
September 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28741466/binding-of-cxcr4-transmembrane-peptides-to-the-bacterial-chaperonin-groel
#14
Xiaoqiang Wang, Haixia Chi, Shixin Li, Yan Xu
: Newly translated membrane protein CXCR4 was observed to gain significant folding enhancement upon interacting with the bacterial chaperonin GroEL-GroES. The main fold of CXCR4 consists of 7 transmembrane α-helices distinct from soluble proteins that have dominated research on the chaperonin-assisted folding. This work extends our previous observation to the binding of GroEL with CXCR4 transmembrane peptides, which are mainly composed of hydrophobic residues characteristic of the substrates recognized by the chaperonin...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28731469/novel-chaperonins-are-prevalent-in-the-virioplankton-and-demonstrate-links-to-viral-biology-and-ecology
#15
Rachel L Marine, Daniel J Nasko, Jeffrey Wray, Shawn W Polson, K Eric Wommack
Chaperonins are protein-folding machinery found in all cellular life. Chaperonin genes have been documented within a few viruses, yet, surprisingly, analysis of metagenome sequence data indicated that chaperonin-carrying viruses are common and geographically widespread in marine ecosystems. Also unexpected was the discovery of viral chaperonin sequences related to thermosome proteins of archaea, indicating the presence of virioplankton populations infecting marine archaeal hosts. Virioplankton large subunit chaperonin sequences (GroELs) were divergent from bacterial sequences, indicating that viruses have carried this gene over long evolutionary time...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28713028/the-effect-of-fusarium-culmorum-infection-and-deoxynivalenol-don-application-on-proteome-response-in-barley-cultivars-chevron-and-pedant
#16
Klára Kosová, Jana Chrpová, Jiří Šantrůček, Radovan Hynek, Lenka Štěrbová, Pavel Vítámvás, Jana Bradová, Ilja Tom Prášil
Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage (BBCH 73) 10days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS investigated 98 protein spots revealing quantitative or qualitative differences between the experimental variants...
July 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28705905/genome-wide-transcriptional-dynamics-in-the-companion-bacterial-symbionts-of-the-glassy-winged-sharpshooter-cicadellidae-homalodisca-vitripennis-reveal-differential-gene-expression-in-bacteria-occupying-multiple-host-organs
#17
Gordon M Bennett, Rebecca A Chong
The agricultural pest known as the glassy-winged sharpshooter (GWSS) or Homalodisca vitripennis (Hemiptera: Cicadellidae) harbors two bacterial symbionts, "Candidatus Sulcia muelleri" and "Ca Baumannia cicadellinicola," which provide the 10 essential amino acids (EAAs) that are limited in the host plant-sap diet. Although they differ in origin and symbiotic age, both bacteria have experienced extensive genome degradation resulting from their ancient restriction to specialized host organs (bacteriomes) that provide cellular support and ensure vertical transmission...
September 7, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28703007/a-chaperonin-nanocaged-hemin-as-an-artificial-metalloenzyme-for-oxidation-catalysis
#18
Xiaoqiang Wang, Chao Wang, Meihong Pan, Junting Wei, Fuping Jiang, Rongsheng Lu, Xuan Liu, Yihui Huang, Fang Huang
Taking inspiration from biology's effectiveness in functionalizing protein-based nanocages for chemical processes, we describe here a rational design of an artificial metalloenzyme for oxidations with the bacterial chaperonin GroEL, a nanocage for protein folding in nature, by supramolecular anchoring of catalytically active hemin in its hydrophobic central cavity. The promiscuity of the chaperonin cavity is an essential element of this design, which can mimic the hydrophobic binding pocket in natural metalloenzymes to accept cofactor and substrate without requiring specific ligand-protein interactions...
July 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28691708/an-exported-protein-interacting-complex-involved-in-the-trafficking-of-virulence-determinants-in-plasmodium-infected-erythrocytes
#19
Steven Batinovic, Emma McHugh, Scott A Chisholm, Kathryn Matthews, Boiyin Liu, Laure Dumont, Sarah C Charnaud, Molly Parkyn Schneider, Paul R Gilson, Tania F de Koning-Ward, Matthew W A Dixon, Leann Tilley
The malaria parasite, Plasmodium falciparum, displays the P. falciparum erythrocyte membrane protein 1 (PfEMP1) on the surface of infected red blood cells (RBCs). We here examine the physical organization of PfEMP1 trafficking intermediates in infected RBCs and determine interacting partners using an epitope-tagged minimal construct (PfEMP1B). We show that parasitophorous vacuole (PV)-located PfEMP1B interacts with components of the PTEX (Plasmodium Translocon of EXported proteins) as well as a novel protein complex, EPIC (Exported Protein-Interacting Complex)...
July 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28667536/natural-monoclonal-antibody-to-oxidized-low-density-lipoprotein-and-aggregatibacter-actinomycetemcomitans
#20
Chunguang Wang, Sohvi Hörkkö
Natural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis...
2017: Methods in Molecular Biology
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