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Chaperonine

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https://www.readbyqxmd.com/read/28445748/directional-force-originating-from-atp-hydrolysis-drives-the-groel-conformational-change
#1
Jie Liu, Kannan Sankar, Yuan Wang, Kejue Jia, Robert L Jernigan
Protein functional mechanisms usually require conformational changes, and often there are known structures for the different conformational states. However, usually neither the origin of the driving force nor the underlying pathways for these conformational transitions is known. Exothermic chemical reactions may be an important source of forces that drive conformational changes. Here we investigate this type of force originating from ATP hydrolysis in the chaperonin GroEL, by applying forces originating from the chemical reaction...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28430446/piecing-together-the-allosteric-patterns-of-chaperonin-groel
#2
Jin Chen, Qian Zhang, Weitong Ren, Wenfei Li
Despite considerable effort, elucidating allostery of large macromolecular assemblies at a molecular level in solution remains technically challenging due to its structural complexity. Here we have employed an approach combining amide backbone hydrogen/deuterium exchange coupled with mass spectrometry, fluorescence spectroscopy and molecular simulations to characterize allosteric patterns of chaperonin GroEL, a ~800 kDa tetradecamer from E. coli. Using available crystal structures of GroEL, we quantitatively map out GroEL allosteric changes in solution by resolving exchange behaviors of 133 overlapping proteolytic peptides with more than 95% sequence coverage...
April 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28424530/molecular-diagnostic-assays-based-on-cpn60-ut-sequences-reveal-the-geographic-distribution-of-subgroup-16srxiii-a-i-i-phytoplasma-in-mexico
#3
Edel Pérez-López, Douglas Rodríguez-Martínez, Chrystel Y Olivier, Mauricio Luna-Rodríguez, Tim J Dumonceaux
Geographically diverse samples from strawberry exhibiting symptoms of Strawberry Green Petal (SbGP), periwinkle plants with virescence, and blackberry, blueberry, and raspberry plants displaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA. PCR targeting the 16S rRNA-encoding gene and chaperonin-60 (cpn60) showed that the plants were infected with phytoplasma subgroup16SrXIII-(A/I)I (SbGP/MPV). To examine the geographic distribution of this pathogen in Mexico, we designed an array of cpn60-targeted molecular diagnostic assays for SbGP/MPV phytoplasma...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28401568/a-secreted-chitinase-like-protein-osclp-supports-root-growth-through-calcium-signaling-in-oryza-sativa
#4
Jingni Wu, Yiming Wang, Sang Gon Kim, Ki-Hong Jung, Ravi Gupta, Joonyup Kim, Younghoon Park, Kyu Young Kang, Sun Tae Kim
Chitinases belong to a conserved protein family and play multiple roles in defense, development, and growth regulation in plants. Here, we identified a secreted chitinase-like protein, OsCLP, which functions in rice growth. A T-DNA insertion mutant of OsCLP (osclp) showed significant retardation of root and shoot growth. A comparative proteomic analysis was carried out using root tissue of wild-type and the osclp mutant to understand the OsCLP-mediated rice growth retardation. Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1) were differentially regulated in osclp roots...
April 12, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28396349/biochemical-and-genetic-analysis-of-the-chlamydia-groel-chaperonins
#5
Melissa Illingworth, Anna J Hooppaw, Lu Ruan, Derek J Fisher, Lingling Chen
Chaperonins are essential for cellular growth under normal and stressful conditions and consequently represent one of the most conserved and ancient protein classes. The paradigm Escherichia coli chaperonin, EcGroEL, and its cochaperonin, EcGroES, assist folding of proteins via an ATP-dependent mechanism. In addition to the presence of groEL and groES homologs, groEL paralogs are found in many bacteria, including pathogens, and have evolved poorly understood species-specific functions. Chlamydia spp., which are obligate intracellular bacteria, have reduced genomes that nonetheless encode three groEL genes, ChgroEL, ChgroEL2 and ChgroEL3 We hypothesized that ChGroEL is the bona-fide chaperonin, while the paralogs perform novel, Chlamydia-specific functions...
April 10, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28378385/functional-characterization-of-lotp-from-liberibacter-asiaticus
#6
Flavia Loto, Janelle F Coyle, Kaylie A Padgett, Fernando A Pagliai, Christopher L Gardner, Graciela L Lorca, Claudio F Gonzalez
Liberibacter asiaticus is an unculturable parasitic bacterium of the alphaproteobacteria group hosted by both citrus plants and a psyllid insect vector (Diaphorina citri). In the citrus tree, the bacteria thrive only inside the phloem, causing a systemically incurable and deadly plant disease named citrus greening or Huanglongbing. Currently, all commercial citrus cultivars in production are susceptible to L. asiaticus, representing a serious threat to the citrus industry worldwide. The technical inability to isolate and culture L...
April 5, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28375158/cct6a-suppresses-smad2-and-promotes-prometastatic-tgf-%C3%AE-signaling
#7
Zhe Ying, Han Tian, Yun Li, Rong Lian, Wei Li, Shanshan Wu, Hui-Zhong Zhang, Jueheng Wu, Lei Liu, Junwei Song, Hongyu Guan, Junchao Cai, Xun Zhu, Jun Li, Mengfeng Li
Paradoxically, during early tumor development in many cancer types, TGF-β acts as a tumor suppressor, whereas in the advanced stages of these cancers, increased TGF-β expression is linked to high metastasis and poor prognosis. These findings suggest that unidentified mechanisms may function to rewire TGF-β signaling toward its prometastatic role in cancer cells. Our current study using non-small-cell lung carcinoma (NSCLC) cell lines, animal models, and clinical specimens demonstrates that suppression of SMAD2, with SMAD3 function intact, switches TGF-β-induced transcriptional responses to a prometastatic state...
April 4, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28331079/characterization-and-vaccine-potential-of-membrane-vesicles-produced-by-francisella-noatunensis-sup-orientalis-in-an-adult-zebrafish-model
#8
Leidy Lagos, Julia I Tandberg, Urska Repnik, Preben Boysen, Erik Ropstad, Deepa Varkey, Ian T Paulsen, Hanne C Winther-Larsen
Vaccine development against extracellular bacteria has been important for the sustainability of the aquaculture industry. In contrast, infections with intracellular pathogens remain largely an unresolved problem. Francisella noatunensis subspecies orientalis (Fno) are Gram-negative, facultative intracellular bacteria that cause the disease francisellosis in fish. Francisellosis is commonly characterized as a chronic granulomatous disease with high morbidity and can result in high mortality depending on the host...
March 22, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28295377/time-restricted-feeding-for-prevention-and-treatment-of-cardiometabolic-disorders
#9
Girish C Melkani, Satchidananda Panda
The soaring prevalence of obesity and diabetes is associated with an increase in comorbidities, including elevated risk for cardiovascular diseases (CVD). CVDs continue to be among the leading causes of death and disability in the United States. While increased nutrition intake from an energy dense diet is known to disrupt metabolic homeostasis and contributes to the disease risk, circadian rhythm disruption is emerging as a new risk factor for CVD. Circadian rhythms coordinate cardiovascular health via temporal control of organismal metabolism and physiology...
March 10, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28283754/leishmania-donovani-chaperonin-10-regulates-parasite-internalization-and-intracellular-survival-in-human-macrophages
#10
Lucie Colineau, Joachim Clos, Kyung-Mee Moon, Leonard J Foster, Neil E Reiner
Protozoa of the genus Leishmania infect macrophages in their mammalian hosts causing a spectrum of diseases known as the leishmaniases. The search for leishmania effectors that support macrophage infection is a focus of significant interest. One such candidate is leishmania chaperonin 10 (CPN10) which is secreted in exosomes and may have immunosuppressive properties. Here, we report for the first time that leishmania CPN10 localizes to the cytosol of infected macrophages. Next, we generated two genetically modified strains of Leishmania donovani (Ld): one strain overexpressing CPN10 (CPN10+++) and the second, a CPN10 single allele knockdown (CPN10+/-), as the null mutant was lethal...
March 11, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28274294/mitochondria-during-sea-urchin-oogenesis
#11
Maria Agnello, Maria Carmela Roccheri, Giovanni Morici, Anna Maria Rinaldi
Sea urchin represents an ideal model for studies on fertilization and early development, but the achievement of egg competence and mitochondrial behaviour during oogenesis remain to be enlightened. Oocytes of echinoid, such as sea urchin, unlike other echinoderms and other systems, complete meiotic maturation before fertilization. Mitochondria, the powerhouse of eukaryotic cells, contain a multi-copy of the maternally inherited genome, and are involved directly at several levels in the reproductive processes, as their functional status influences the quality of oocytes and contributes to fertilization and embryogenesis...
March 9, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28271487/groel-and-the-groel-groes-complex
#12
Noriyuki Ishii
Chaperonin is categorized as a molecular chaperone and mediates the formation of the native conformation of proteins by first preventing folding during synthesis or membrane translocation and subsequently by mediating the step-wise ATP-dependent release that result in proper folding. In the GroEL-GroES complex, a single heptameric GroEL ring binds one GroES ring in the presence of ATP/ADP, in this vein, the double ring GroEL tetradecamer is present in two distinct types of GroEL-GroES complexes: asymmetric 1:1 "bullet"-shaped GroEL:GroES and symmetric 1:2 "football" (American football)-shaped GroEL:GroES2...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28257512/quantitative-molecular-diagnostic-assays-of-grain-washes-for-claviceps-purpurea-are-correlated-with-visual-determinations-of-ergot-contamination
#13
Alexia Comte, Tom Gräfenhan, Matthew G Links, Sean M Hemmingsen, Tim J Dumonceaux
We examined the epiphytic microbiome of cereal grain using the universal barcode chaperonin-60 (cpn60). Microbial community profiling of seed washes containing DNA extracts prepared from field-grown cereal grain detected sequences from a fungus identified only to Class Sordariomycetes. To identify the fungal sequence and to improve the reference database, we determined cpn60 sequences from field-collected and reference strains of the ergot fungus, Claviceps purpurea. These data allowed us to identify this fungal sequence as deriving from C...
2017: PloS One
https://www.readbyqxmd.com/read/28246357/lethal-consequences-of-overcoming-metabolic-restrictions-imposed-on-a-cooperative-bacterial-population
#14
Eunhye Goo, Yongsung Kang, Jae Yun Lim, Hyeonheui Ham, Ingyu Hwang
Quorum sensing (QS) controls cooperative activities in many Proteobacteria In some species, QS-dependent specific metabolism contributes to the stability of the cooperation. However, the mechanism by which QS and metabolic networks have coevolved to support stable public good cooperation and maintenance of the cooperative group remains unknown. Here we explored the underlying mechanisms of QS-controlled central metabolism in the evolutionary aspects of cooperation. In Burkholderia glumae, the QS-dependent glyoxylate cycle plays an important role in cooperativity...
February 28, 2017: MBio
https://www.readbyqxmd.com/read/28178129/chaperonin-hsp60-and-annexin-2-are-candidate-biomarkers-for-non-small-cell-lung-carcinoma
#15
İsmail Ağababaoğlu, Ahmet Önen, Ayşe Banu Demir, Safiye Aktaş, Zekiye Altun, Hasan Ersöz, Aydn Şanl, Nezih Özdemir, Atila Akkoçlu
BACKGROUND: Lung cancer is responsible of 12.4% and 17.6% of all newly diagnosed cancer cases and mortality due to cancer, respectively, and 5-year survival rate despite all improved treatment options is 15%. This survival rate reaches 66% in the Stage 1 and surgically treated patients. Early diagnosis which could not be definitely and commonly achieved yet is extremely critical in obtaining high survival rate in this disease. For this reason; proteomic differences were evaluated using matrix assisted laser desorption ionization (MALDI) mass spectrometry in the subgroups of lung adenocarcinoma and squamous cell carcinoma...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28156097/confinement-and-stabilization-of-fyn-sh3-folding-intermediate-mimetics-within-the-cavity-of-the-chaperonin-groel-demonstrated-by-relaxation-based-nmr
#16
David S Libich, Vitali Tugarinov, Rodolfo Ghirlando, G Marius Clore
The interaction of two folding intermediate mimetics of the model protein substrate Fyn SH3 with the chaperonin GroEL, a supramolecular foldase/unfoldase machine, has been investigated by (15)N relaxation-based nuclear magnetic resonance spectroscopy (lifetime line broadening, dark state exchange saturation transfer, and relaxation dispersion). The two mimetics comprise C-terminal truncations of wild-type and triple-mutant (A39V/N53P/V55L) Fyn SH3 in which the C-terminal strand of the SH3 domain is unfolded, while preserving the remaining domain structure...
February 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28143946/cellular-proteomes-drive-tissue-specific-regulation-of-the-heat-shock-response
#17
Jian Ma, Christopher E Grant, Rosemary N Plagens, Lindsey N Barrett, Karen S Kim Guisbert, Eric Guisbert
The heat shock response (HSR) is a cellular stress response that senses protein misfolding and restores protein folding homeostasis, or proteostasis. We previously identified an HSR regulatory network in Caenorhabditis elegans consisting of highly conserved genes that have important cellular roles in maintaining proteostasis. Unexpectedly, the effects of these genes on the HSR are distinctly tissue-specific. Here, we explore this apparent discrepancy and find that muscle-specific regulation of the HSR by the TRiC/CCT chaperonin is not driven by an enrichment of TRiC/CCT in muscle, but rather by the levels of one of its most abundant substrates, actin...
March 10, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28126905/a-trimer-consisting-of-the-tubulin-specific-chaperone-d-tbcd-regulatory-gtpase-arl2-and-%C3%AE-tubulin-is-required-for-maintaining-the-microtubule-network
#18
Joshua W Francis, Laura E Newman, Leslie A Cunningham, Richard A Kahn
Microtubule dynamics involves the polymerization and depolymerization of tubulin dimers and is an essential and highly regulated process required for cell viability, architecture, and division. The regulation of the microtubule network also depends on the maintenance of a pool of αβ-tubulin heterodimers. These dimers are the end result of complex folding and assembly events, requiring the TCP1 Ring Complex (TriC or CCT) chaperonin and five tubulin-specific chaperones, tubulin binding cofactors A-E (TBCA-TBCE)...
March 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28125284/biogenesis-and-metabolic-maintenance-of-rubisco
#19
Andreas Bracher, Spencer M Whitney, F Ulrich Hartl, Manajit Hayer-Hartl
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) mediates the fixation of atmospheric CO2 in photosynthesis by catalyzing the carboxylation of the 5-carbon sugar ribulose-1,5-bisphosphate (RuBP). Rubisco is a remarkably inefficient enzyme, fixing only 2-10 CO2 molecules per second. Efforts to increase crop yields by bioengineering Rubisco remain unsuccessful, owing in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. The large subunit of Rubisco requires the chaperonin system for folding, and recent studies have shown that assembly of hexadecameric Rubisco is mediated by specific assembly chaperones...
January 11, 2017: Annual Review of Plant Biology
https://www.readbyqxmd.com/read/28108864/is-raf1-protein-from-synechocystis-sp-pcc-6803-really-needed-in-the-cyanobacterial-rubisco-assembly-process
#20
Piotr Kolesinski, Malgorzata Rydzy, Andrzej Szczepaniak
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is responsible for carbon dioxide conversion during photosynthesis and, therefore, is the most important protein in biomass generation. Modifications of this biocatalyst toward improvements in its properties are hindered by the complicated and not yet fully understood assembly process required for the formation of active holoenzymes. An entire set of auxiliary factors, including chaperonin GroEL/GroES and assembly chaperones RbcX or Rubisco accumulation factor 1 (RAF1), is involved in the folding and subsequent assembly of Rubisco subunits...
January 20, 2017: Photosynthesis Research
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