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https://www.readbyqxmd.com/read/27916650/cardiac-oxidative-stress-in-diabetes-mechanisms-and-therapeutic-potential
#1
REVIEW
Alyssa Faria, Shanta J Persaud
Macrovascular complications of diabetes, including diabetic cardiovascular disease (CVD), occur through a number of hyperglycaemia-induced mechanisms that include generation of oxidative stress, accumulation of advanced glycation end-products (AGE) and activation of protein kinase C (PKC). Cardiac oxidative stress is associated with increased cardiac fibrosis and hypertrophy, and reduced cardiac performance and contractility, leading to severe cardiac dysfunction and potentially fatal cardiac events. It occurs under conditions of excessive synthesis of reactive oxygen species (ROS)...
December 1, 2016: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27893755/quantification-of-arachidonic-acid-and-its-metabolites-in-rat-tissues-by-uhplc-ms-ms-application-for-the-identification-of-potential-biomarkers-of-benign-prostatic-hyperplasia
#2
Qiaoxia Bian, Weihui Wang, Nannan Wang, Yan Peng, Wen Ma, Ronghua Dai
To evaluate the potential relationship between benign prostatic hyperplasia (BPH) and the arachidonic acid (AA) metabolome, a UHPLC-MS/MS method has been developed and validated for simultaneous determination of AA and its cyclooxygenase(COX) and lipoxygenase(LOX) pathway metabolites (15-HETE, 12-HETE, TXA2, 5-HETE, AA, PGI2, PGF2α, 8-HETE, PGD2, PGE2 and LTB4) in rat tissues. The analytes were extracted from tissue samples with a protein precipitation procedure and then separated on a Shim-pack XR-ODSC18 column with 0...
2016: PloS One
https://www.readbyqxmd.com/read/27836541/substituted-e-2-2-benzylidenehydrazinyl-4-methylthiazole-5-carboxylates-as-dual-inhibitors-of-15-lipoxygenase-carbonic-anhydrase-ii-synthesis-biological-evaluation-and-docking-studies
#3
Aamer Saeed, Shafi Ullah Khan, Parvez Ali Mahesar, Pervaiz Ali Channar, Ghulam Shabir, Jamshed Iqbal
15-Lipoxygenase (15-LOX) plays a major role in many inflammatory lung diseases including chronic obstructive pulmonary disease (COPD), asthma and chronic bronchitis. Over-expression of 15-LOX is related with some specific carcinomas including pancreatic, gastric and brain tumor. Similarly among different isozymes of carbonic anhydrase (CA), CA II is expressed in pancreatic, gastric carcinomas as well as in brain tumors. Therefore, novel potent inhibitors of both 15-LOX and CA II are required to explore the role of these enzymes further and to enable the drug discovery efforts...
November 8, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27832777/a-proteomic-analysis-of-acute-leukemia-cells-treated-with-9-hydroxyoctadecadienoic-acid
#4
Zhen Li, Bohong Chen, Ping Wang, Xin Li, Gaotai Cai, Wei Wei, Wenqi Dong
BACKGROUND: 9s-hydroxy-octadecadienoic acid (9S-HOD), one of the natural products of linoleic acid oxygenation by 15-lipoxygenase (15-LOX), has been found to have anti-tumor properties in vitro and in vivo. The present study therefore investigated whether 9S-HOD affects acute leukemia HL-60 cells. METHODS: The cytotoxicity of 9S-HOD in HL-60 with or without the presence of fetal bovine serum (FBS) in the culture media was tested using cell viability assays and flow cytometry...
November 10, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27826169/esculetin-reduces-leukotriene-b4-level-in-plasma-of-rats-with-adjuvant-induced-arthritis
#5
Przemysław Rzodkiewicz, Emilia Gąsińska, Michał Gajewski, Magdalena Bujalska-Zadrożny, Dariusz Szukiewicz, Sławomir Maśliński
OBJECTIVES: Esculetin (6,7-dihydroxycoumarin) is a natural coumarin with anti-oxidant, anti-inflammatory and anti-nociceptive activity. It acts as a potent inhibitor of lipoxygenases (5-LOX and 12-LOX) and decreases the production of matrix metalloproteinases (MMP-1, MMP-3 and MMP-9). Because both inhibition of lipoxygenases and inhibition of matrix metalloproteinases are effective strategies in the treatment of rheumatoid arthritis, we investigated whether esculetin may be effective in adjuvant-induced arthritis in rats...
2016: Reumatologia
https://www.readbyqxmd.com/read/27825104/human-15-lipoxygenase-1-is-a-regulator-of-dendritic-cell-spreading-and-podosome-formation
#6
Hongya Han, Xiuming Liang, Monica Ekberg, Joanna S Kritikou, Åsa Brunnström, Benjamin Pelcman, Maria Matl, Xinyan Miao, Margareta Andersson, Xiaotian Yuan, Frida Schain, Selina Parvin, Eva Melin, Jan Sjöberg, Dawei Xu, Lisa S Westerberg, Magnus Björkholm, Hans-Erik Claesson
Dendritic cells (DCs) involved in proinflammatory immune responses derive mainly from peripheral monocytes, and the cells subsequently mature and migrate into the inflammatory micromilieu. Here we report that suppressing of 15-lipoxygenase-1 led to a substantial reduction in DC spreading and podosome formation in vitro. The surface expression of CD83 was significantly lower in both sh-15-lipoxygenase-1 (15-LOX-1)-transduced cells and DCs cultivated in the presence of the inhibitor. The T-cell response against tetanus-pulsed DCs was only affected to a minor extent on inhibition of 15-LOX-1...
October 17, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27818578/apoptosis-of-endothelial-cells-by-13-hpode-contributes-to-impairment-of-endothelial-barrier-integrity
#7
Valerie E Ryman, Nandakumar Packiriswamy, Lorraine M Sordillo
Inflammation is an essential host response during bacterial infections such as bovine mastitis. Endothelial cells are critical for an appropriate inflammatory response and loss of vascular barrier integrity is implicated in the pathogenesis of Streptococcus uberis-induced mastitis. Previous studies suggested that accumulation of linoleic acid (LA) oxygenation products derived from 15-lipoxygenase-1 (15-LOX-1) metabolism could regulate vascular functions. The initial LA derivative from the 15-LOX-1 pathway, 13-hydroperoxyoctadecadienoic acid (HPODE), can induce endothelial death, whereas the reduced hydroxyl product, 13-hydroxyoctadecadienoic acid (HODE), is abundantly produced during vascular activation...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27814242/neonatal-cord-blood-oxylipins-and-exposure-to-particulate-matter-in-the-early-life-environment-an-environage-birth-cohort-study
#8
Dries S Martens, Sandra Gouveia, Narjes Madhloum, Bram G Janssen, Michelle Plusquin, Charlotte Vanpoucke, Wouter Lefebvre, Bertil Forsberg, Malin Nording, Tim S Nawrot
BACKGROUND: As part of the lipidome, oxylipins are bioactive lipid compounds originating from oxidation of different fatty acids. Oxylipins could provide a new target in the developmental origins model or the ability of early life exposure to change biology. OBJECTIVES: We studied the association between in utero PM2.5 (particulate matter with aerodynamic diameter <2.5µm) exposure and oxylipin profiles in newborns. METHODS: Thirty-seven oxylipins reflecting the cyclooxygenase (COX), lipoxygenase (5-LOX and 12/15-LOX) and cytochrome P450 (CYP) pathways were assayed in 197 cord blood plasma samples from the ENVIRONAGE birth cohort...
November 4, 2016: Environmental Health Perspectives
https://www.readbyqxmd.com/read/27802026/magnetic-microbead-affinity-selection-screening-magmass-of-botanical-extracts-for-inhibitors-of-15-lipoxygenase
#9
Michael D Rush, Elisabeth M Walker, Tristesse Burton, Richard B van Breemen
To expedite the identification of active natural products in complex mixtures such as botanical extracts, a magnetic microbead affinity selection screening (MagMASS) procedure was developed. This technique utilizes target proteins immobilized on magnetic beads for rapid bioaffinity isolation of ligands from complex mixtures. A MagMASS method was developed and validated for 15-lipoxygenase. As a proof of concept, several North American prairie plants used medicinally by Native Americans were extracted with MeOH and screened...
November 1, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/27777380/aging-dysregulates-d-and-e-series-resolvins-to-modulate-cardiosplenic-and-cardiorenal-network-following-myocardial-infarction
#10
Ganesh V Halade, Vasundhara Kain, Laurence M Black, Sumanth D Prabhu, Kevin A Ingle
Post-myocardial infarction (MI), overactive inflammation is the hallmark of aging, however, the mechanism is unclear. We hypothesized that excess influx of omega 6 fatty acids may impair resolution, thus impacting the cardiosplenic and cardiorenal network post-MI. Young and aging mice were fed on standard lab chow (LC) and excess fatty acid (safflower oil; SO)-enriched diet for 2 months and were then subjected to MI surgery. Despite similar infarct areas and left ventricle (LV) dysfunction post-MI, splenic mass spectrometry data revealed higher levels of arachidonic acid (AA) derived pro-inflammatory metabolites in young-SO, but minimal formation of docosanoids, D- and E- series resolvins in SO-fed aged mice...
October 18, 2016: Aging
https://www.readbyqxmd.com/read/27777006/inhibition-of-plasma-lipid-oxidation-induced-by-peroxyl-radicals-peroxynitrite-hypochlorite-15-lipoxygenase-and-singlet-oxygen-by-clinical-drugs
#11
Mayuko Morita, Yuji Naito, Toshikazu Yoshikawa, Etsuo Niki
With increasing evidence showing the involvement of oxidative stress in the pathogenesis of various diseases, the effects of clinical drugs possessing antioxidant functions have received much attention. The unregulated oxidative modification of biological molecules leading to diseases is mediated by multiple oxidants including free radicals, peroxynitrite, hypochlorite, lipoxygenase, and singlet oxygen. The capacity of antioxidants to scavenge or quench oxidants depends on the nature of oxidants. In the present study, the antioxidant effects of several clinical drugs against plasma lipid oxidation induced by the aforementioned five kinds of oxidants were investigated from the production of lipid hydroperoxides, which have been implicated in the pathogenesis of various diseases...
October 13, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27739938/activation-of-peroxisome-proliferator-activated-receptor-%C3%AE-by-a-12-15-lipoxygenase-product-of-arachidonic-acid-a-possible-neuroprotective-effect-in-the-brain-after-experimental-intracerebral-hemorrhage
#12
Ruobing Xu, Shu Wang, Weishan Li, Zhen Liu, Jiaxin Tang, Xiaobo Tang
OBJECTIVE In this study, the authors investigated the involvement of 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE) in the regulation of peroxisome proliferator-activated receptor-γ (PPARγ) after intracerebral hemorrhage (ICH) and its effects on hemorrhage-induced inflammatory response and oxidative stress in an experimental rodent model. METHODS To simulate ICH in a rat model, the authors injected autologous whole blood into the right striatum of male Sprague-Dawley rats. The distribution and expression of 12/15-lipoxygenase (12/15-LOX) were determined by immunohistochemistry and Western blot analysis, respectively...
October 14, 2016: Journal of Neurosurgery
https://www.readbyqxmd.com/read/27732897/immobilization-and-delivery-of-biologically-active-lipoxin-a4-using-electrospinning-technology
#13
Eleonora Cianci, Oriana Trubiani, Francesca Diomede, Ilaria Merciaro, Ida Meschini, Pantaleone Bruni, Fausto Croce, Mario Romano
Lipoxin (LX)A4 is a lipoxygenase-formed arachidonic acid metabolite with potent anti-inflammatory, pro-resolution properties. Its therapeutic efficacy has been largely demonstrated in a variety of cellular, preclinical and clinical models. Among these, periodontal disease, where LXA4 promotes tissue repair, also by modulating functions of human periodontal ligament stem cells (hPDLSCs). As medicated biomembranes may be particularly useful in clinical settings, where local stimulation of tissue repair is needed, we used electrospinning to embed LXA4 in membranes made of poly(ethylene oxide) (PEO) and poly(d,l-lactide) (PDLLA)...
October 11, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27698118/activation-of-sat1-engages-polyamine-metabolism-with-p53-mediated-ferroptotic-responses
#14
Yang Ou, Shang-Jui Wang, Dawei Li, Bo Chu, Wei Gu
Although p53-mediated cell-cycle arrest, senescence, and apoptosis remain critical barriers to cancer development, the emerging role of p53 in cell metabolism, oxidative responses, and ferroptotic cell death has been a topic of great interest. Nevertheless, it is unclear how p53 orchestrates its activities in multiple metabolic pathways into tumor suppressive effects. Here, we identified the SAT1 (spermidine/spermine N(1)-acetyltransferase 1) gene as a transcription target of p53. SAT1 is a rate-limiting enzyme in polyamine catabolism critically involved in the conversion of spermidine and spermine back to putrescine...
November 1, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27696115/the-analgesic-and-anxiolytic-effect-of-souvenaid-a-novel-nutraceutical-is-mediated-by-alox15-activity-in-the-prefrontal-cortex
#15
Suku-Maran Shalini, Deron R Herr, Wei-Yi Ong
Pain and anxiety have a complex relationship and pain is known to share neurobiological pathways and neurotransmitters with anxiety. Top-down modulatory pathways of pain have been shown to originate from cortical and subcortical regions, including the dorsolateral prefrontal cortex. In this study, a novel docosahexaenoic acid (DHA)-containing nutraceutical, Souvenaid, was administered to mice with infraorbital nerve ligation-induced neuropathic pain and behavioral responses recorded. Infraorbital nerve ligation resulted in increased face wash strokes of the face upon von Frey hair stimulation, indicating increased nociception...
October 1, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27681120/altering-the-anti-inflammatory-lipoxin-microenvironment-a-new-insight-into-kaposi-s-sarcoma-associated-herpesvirus-pathogenesis
#16
Jayashree A Chandrasekharan, Xiao M Huang, Alexander C Hwang, Neelam Sharma-Walia
: Lipoxins are host anti-inflammatory molecules that play a vital role in restoring tissue homeostasis. The efficacy of lipoxins and their analog epilipoxins in treating inflammation and its associated diseases has been well documented. Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) are two well-known inflammation related diseases caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Controlling inflammation is one of the strategies adopted to treat KS and PEL, a primary motivation for exploring and evaluating the therapeutic potential of using lipoxins...
December 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27665716/caffeic-acid-morin-hydrate-and-quercetin-partially-attenuate-sulfur-mustard-induced-cell-death-by-inhibiting-the-lipoxygenase-pathway
#17
Shin Kim, Kwang-Joon Jeong, Sung Kweon Cho, Joo-Won Park, Woo-Jae Park
Sulfur mustard (SM) is an alkylating agent, which has been used as in chemical warfare in a number of conflicts. As the generation of reactive oxygen species (ROS), and adducts in DNA and proteins have been suggested as the mechanism underlying SM‑induced cytotoxicity, the present study screened several antioxidant candidates, including tannic acid, deferoxamine mesylate, trolox, vitamin C, ellagic acid and caffeic acid (CA) to assess their potential as therapeutic agents for SM‑induced cell death. Among several antioxidants, CA partially alleviated SM‑induced cell death in a dose‑dependent manner...
September 23, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27650786/chemical-composition-and-biological-activities-of-extracts-and-essential-oil-of-boswellia-dalzielii-leaves
#18
Midéko Justin Kohoude, Fernand Gbaguidi, Pierre Agbani, Marc-Abel Ayedoun, Sylvie Cazaux, Jalloul Bouajila
CONTEXT: Boswellia dalzielii Hutch. (Burseraceae) is an aromatic plant. The leaves are used for beverage flavouring. OBJECTIVE: This study investigates the chemical composition and biological activities of various extracts. MATERIALS AND METHODS: The essential oil was prepared via hydrodistillation. Identification and quantification were realized via GC-MS and GC-FID. Consecutive extractions (cyclohexane, dichloromethane, ethyl acetate and methanol) were carried out and various chemical groups (phenolics, flavonoids, tannins, antocyanins and sugar) were quantified...
September 21, 2016: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27647374/human-15-lox-1-active-site-mutations-alter-inhibitor-binding-and-decrease-potency
#19
Michelle Armstrong, Christopher van Hoorebeke, Thomas Horn, Joshua Deschamps, J Cody Freedman, Chakrapani Kalyanaraman, Matthew P Jacobson, Theodore Holman
Human 15-lipoxygenase-1 (h15-LOX-1 or h12/15-LOX) reacts with polyunsaturated fatty acids and produces bioactive lipid derivatives that are implicated in many important human diseases. One such disease is stroke, which is the fifth leading cause of death and the first leading cause of disability in America. The discovery of h15-LOX-1 inhibitors could potentially lead to novel therapeutics in the treatment of stroke, however, little is known about the inhibitor/active site interaction. This study utilizes site-directed mutagenesis, guided in part by molecular modeling, to gain a better structural understanding of inhibitor interactions within the active site...
November 1, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27647014/flavonoids-gallotannins-and-ellagitannins-in-syzygium-guineense-and-the-traditional-use-among-malian-healers
#20
Thuy Lan Nguyen, Anders Rusten, Mona Skogsrud Bugge, Karl Egil Malterud, Drissa Diallo, Berit Smestad Paulsen, Helle Wangensteen
ETHNOPHARMACOLOGICAL RELEVANCE: Syzygium guineense has been traditionally used in Mali in West Africa for the treatment of different diseases such as stomach problems, wounds, inflammations and various female disorders. AIMS OF THE STUDY: (1) To perform an ethnopharmacological survey on the traditional use of S. guineense among Malian healers. (2) To isolate and identify chemical constituents from S. guineense leaves and to study their radical scavenging and enzyme inhibitory effects...
November 4, 2016: Journal of Ethnopharmacology
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