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https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#1
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28109129/vsl-3-probiotic-differently-influence-iec-6-intestinal-epithelial-cell-status-and-function
#2
REVIEW
Benedetta Cinque, Cristina La Torre, Francesca Lombardi, Paola Palumbo, Zoran Evtoski, Silvano Jr Santini, Stefano Falone, Annamaria Cimini, Fernanda Amicarelli, Maria Grazia Cifone
The data here reported introduce the wound-healing assay as a tool for testing probiotics aimed at protecting gastrointestinal mucosal surfaces and to verify the consistency of their manufacturing. At the scope, we compared the in vitro effects of two multi-strain high concentration formulations both commercialized under the same brand VSL#3 but sourced from different production sites (USA and Italy) on a non-transformed small-intestinal epithelial cell line, IEC-6. The effects on cellular morphology, viability, migration, and H2 O2 -induced damage, were assessed before and after the treatment with both VSL#3 formulations...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28109097/-mucosal-healing-in-different-intestinal-segments-in-patients-receiving-infliximab-treatment-for-small-bowel-crohn-s-disease
#3
Zhen-Hao Zhu, Chen Qiu, Ming Zhang, Zhao Chen, Cheng Xiang, Xin-Ying Wang
OBJECTIVE: To evaluate the mucosal healing in the terminal ileum, colon and small bowel in patients receiving infliximab treatment for small bowel Crohn's disease (SBCD). METHODS: The clinical data of 18 patients with SBCD treated with infliximab were analyzed for laboratory findings (routine blood tests, C-reative protein, and albumin), Crohn's disease activity index (CDAI), Lewis score (LS), Crohn's disease simplified endoscopic score (SES-CD) and adverse effects before and after 30 weeks of infliximab treatment...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109092/-early-outcomes-of-elective-surgery-for-colon-cancer-with-preoperative-mechanical-bowel-preparation-a-randomized-clinical-trial
#4
Yan-Jie Hu, Ka Li, Li Li, Xiao-Dong Wang, Jie Yang, Jin-Hua Feng, Wei Zhang, Yu-Wei Liu
OBJECTIVE: To compared the early outcomes of patients undergoing elective surgeries for colon cancer with and without preoperative mechanical bowel preparation. METHODS: Between July, 2014 and February, 2016, patients undergoing elective surgery for colon cancer with primary anastomosis were randomly assigned into control group with mechanical bowel preparation 12 h before surgery and treatment group without mechanical bowel preparation. Baseline data collection was completed within 12 h after the operation...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109091/-effect-of-deoxycholic-acid-intervention-on-growth-of-ileum-organoids-derived-from-c57bl-6-mice
#5
Li-Hong Lou, Yue Zeng, Hui Zhou, Ying-Ying Lu, Xing-Peng Wang
OBJECTIVE: To establish a culture system for mouse intestinal organoids and investigate the effect of deoxycholic acid (DCA) on organoids growth. METHODS: The terminal ileum was collected from 8-month-old C57BL<6 mice. The tissue blocks were treated with EDTA and the crypts were collected and embedded in Matrigel Matrix. Orgnoids growth and buddings were observed in the control group, anhydrous alcohol group, short-term (2 days) 100 µmol<L DCA treatment group, and long-term (10 days) 10 µmol<L DCA treatment group; the orgnoids were further cultured for 10 days after removal of DCA from the medium and observed for orgnoids growth and buddings...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109045/misoprostol-for-cervical-priming-prior-to-hysteroscopy-in-postmenopausal-and-premenopausal-nulliparous-women-a-multicentre-randomised-placebo-controlled-trial
#6
M L Tasma, M Louwerse, W J Hehenkamp, P M Geomini, M Y Bongers, S Veersema, P J van Kesteren, E Tromp, J A Huirne, G C Graziosi
OBJECTIVE: To evaluate the reduction of pain by Misoprostol compared to placebo prior to hysteroscopy in postmenopausal and premenopausal nulliparous women. DESIGN: Randomised multicentre double-blind placebo controlled trial. SETTING: Two Dutch teaching hospitals and one Dutch university medical centre. POPULATION: Postmenopausal and premenopausal nulliparous women undergoing office hysteroscopy. METHODS: Patients were randomised to receive either 400 mcg Misoprostol or placebo 12 and 24 hours before hysteroscopy...
January 21, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28109017/clostridium-butyricum-b1-alleviates-high-fat-diet-induced-steatohepatitis-in-mice-via-enterohepatic-immunoregulation
#7
Da Zhou, Qin Pan, Xiao-Lin Liu, Rui-Xu Yang, Yuan-Wen Chen, Chang Liu, Jian-Gao Fan
BACKGROUND AND AIM: Enterohepatic immunologic derangement is associated with nonalcoholic steatohepatitis (NASH). Here, we investigated whether Clostridium butyricum B1 (CB) would be an effective immune-targeted substance to attenuate steatohepatitis in mice. METHODS: Thirty mice were randomized into a control group fed with common forage, a high-fat diet (HFD) group fed a HFD for 16 weeks, and a HFD + CB group treated with CB for the latter 8 weeks. Inflammation- or metabolism-associated genes in the liver or epididymal fat tissue were quantified, intrahepatic and intestinal immune factors were detected...
January 20, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28108908/diamine-oxidase-from-white-pea-lathyrus-sativus-combined-with-catalase-protects-the-human-intestinal-caco-2-cell-line-from-histamine-damage
#8
Catherine Jumarie, Marilyne Séïde, Lucia Marcocci, Paola Pietrangeli, Mircea Alexandru Mateescu
Diamine oxidase (DAO) administration has been proposed to treat certain gastrointestinal dysfunctions induced by histamine, an immunomodulator, signaling, and pro-inflammatory factor. However, H2O2 resulting from the oxidative deamination of histamine by DAO may be toxic. The purpose of this study was to investigate to which extent DAO from white pea (Lathyrus sativus), alone or in combination with catalase, may modulate histamine toxicity in the human intestinal Caco-2 cell line. The results show that histamine at concentrations higher than 1 mM is toxic to the Caco-2 cells, independently of the cell differentiation status, with a LC50 of ≅ 10 mM following a 24-h exposure...
January 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28108644/bicarbonate-sensing-soluble-adenylyl-cyclase-is-present-in-the-cell-cytoplasm-and-nucleus-of-multiple-shark-tissues
#9
Jinae N Roa, Martin Tresguerres
The enzyme soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate (HCO3(-)) to produce the signaling molecule cyclic adenosine monophosphate (cAMP). Because sAC and sAC-related enzymes are found throughout phyla from cyanobacteria to mammals and they regulate cell physiology in response to internal and external changes in pH, CO2, and HCO3(-), sAC is deemed an evolutionarily conserved acid-base sensor. Previously, sAC has been reported in dogfish shark and round ray gill cells, where they sense and counteract blood alkalosis by regulating the activity of V-type H(+)- ATPase...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28108624/inflammatory-molecule-psgl-1-deficiency-activates-macrophages-to-promote-colorectal-cancer-growth-through-nf-kappab-signaling
#10
Jiangchao Li, Zeqi Zhou, Xiaohan Zhang, Li Zheng, Dan He, Yuxiang Ye, Qian-Qian Zhang, Cui-Ling Qi, Xiao-Dong He, Chen Yu, Chun-Kui Shao, Liang Qiao, Lijing Wang
: P-selectin glycoprotein ligand 1 (SELPLG/PSGL-1) is an inflammatory molecule that is functionally related to immune cell differentiation and leukocyte mobilization. However, the role of PSGL-1 in tumor development remains unknown. Therefore, this study investigates the mechanistic role of PSGL-1 in the development of intestinal tumors in colorectal cancer (CRC). ApcMin/+ mice, are highly susceptible to spontaneous intestinal adenoma formation, and were crossbred with PSGL1-null mice to generate compound transgenic mice with a ApcMin/+;PSGL-1-/- genotype...
January 20, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28108553/the-bright-fluorescent-protein-mneongreen-facilitates-protein-expression-analysis-in-vivo
#11
Lola Hostettler, Laura Grundy, Stéphanie Käser-Pébernard, Chantal Wicky, William R Schafer, Dominique A Glauser
The Green Fluorescent Protein (GFP) has been tremendously useful to investigate cell architecture, protein localization, and protein function. While recent developments in transgenesis and genome editing methods enable working with few transgene copies and, consequently, with physiological expression levels, lower signal intensity might become a limiting factor. The recently developed mNeonGreen protein is a brighter alternative to GFP in vitro The goal of the present study was to determine how mNeonGreen performs in vivo in Caenorhabditis elegans, a model extensively used for fluorescence imaging in intact animals...
January 20, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28108413/investigation-of-pectin-starch-hydrogel-as-a-carrier-for-oral-delivery-of-probiotic-bacteria
#12
Alireza Dafe, Hossein Etemadi, Azita Dilmaghani, Gholam Reza Mahdavinia
The present study highlights the fabrication of novel food-grade hydrogel particles based on pectin and starch for probiotic colon delivery. Lactobacillus plantarum ATCC:13643 (L. plantarum) cells were encapsulated in pectin/starch hydrogel by extrusion method. Four batches were formulated with different ratios of starch/pectin solutions. Optical and scanning electron microscopy obviously showed the random distribution of L. plantarum throughout the hydrogel network. The viability of encapsulated cells in simulated gastric fluid (SGF) and bile salt solution was significantly higher when compared to nonencapsulated cells...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108361/development-and-characterization-of-new-functionalized-polyurethanes-for-sustained-and-site-specific-drug-release-in-the-gastrointestinal-tract
#13
María Dolores Campiñez, Elena Benito, Lucía Romero-Azogil, Ángela Aguilar-de-Leyva, María de Gracia García-Martín, Juan Antonio Galbis, Isidoro Caraballo
The main objective of the present paper has been the development and study of two new biodegradable polyurethanes, PU(dithiodiethanol-DTDI) and PU[((i)Pr)Man-DTDI], to be used as sustained matrix forming excipients. Furthermore, their capacity to act as excipient for colon drug delivery systems has been evaluated. Thus, SeDeM diagrams have been obtained to investigate their suitability to be processed through a direct compression process. Matrices containing 10-30% w/w of the polymers and theophylline anhydrous as model drug have been manufactured...
January 17, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28108360/archaeal-lipids-in-oral-delivery-of-therapeutic-peptides
#14
Ann-Christin Jacobsen, Sara M Jensen, Gert Fricker, Martin Brandl, Alexander Treusch
Archaea contain membrane lipids that differ from those found in the other domains of life (Eukarya and Bacteria). These lipids consist of isoprenoid chains attached via ether bonds to the glycerol carbons at the sn-2,3 positions. Two types of ether lipids are known, polar diether lipids and bipolar tetraether lipids. The inherent chemical stability and unique membrane-spanning characteristics of tetraether lipids render them interesting for oral drug delivery purposes. Archaeal lipids form liposomes spontaneously (archaeosomes) and may be incorporated in conventional liposomes (mixed vesicles)...
January 17, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28107883/batch-medication-of-intestinal-infections-in-nursery-pigs-a-randomised-clinical-trial-on-the-efficacy-of-treatment-strategy-type-of-antibiotic-and-bacterial-load-on-average-daily-weight-gain
#15
Nicolai Rosager Weber, Ken Steen Pedersen, Christian Fink Hansen, Matthew Denwood, Charlotte Kristiane Hjulsager, Jens Peter Nielsen
INTRODUCTION: Previous research projects have demonstrated the need for better diagnostic tools to support decisions on medication strategies for infections caused by Escherichia coli F4 (F4) and F18 (F18), Lawsonia intracellularis (LI) and Brachyspira pilosicoli (PILO). This study was carried out as a randomised clinical trial in three Danish pig herds and included 1047 nursery pigs, distributed over 10 batches and 78 pens. The objectives of this study were: (1) to assess the effect of four 5-day treatment strategies (initiated at clinical outbreak of diarrhoea or at fixed time points 14, 21, or 28days after weaning) on average daily weight gain (ADG); (2) to compare the effect of treatment with doxycycline or tylosine on diarrhoea prevalence, pathogenic bacterial load, and ADG; (3) to evaluate PCR testing of faecal pen floor samples as a diagnostic tool for determining the optimal time of treatment...
February 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28107878/effect-of-strategic-administration-of-an-encapsulated-blend-of-formic-acid-citric-acid-and-essential-oils-on-salmonella-carriage-seroprevalence-and-growth-of-finishing-pigs
#16
Kavita Walia, Hector Argüello, Helen Lynch, Finola C Leonard, Jim Grant, Dermot Yearsley, Sinead Kelly, Geraldine Duffy, Gillian E Gardiner, Peadar G Lawlor
Controlling Salmonella at farm level can act as the first line of defence in reducing salmonellosis from pork. This study investigated the efficacy of an encapsulated blend of formic acid, citric acid, and essential oils (FormaXOL™) administered to finisher pigs for 28days prior to slaughter in controlling Salmonella shedding on a commercial farm with a history of high Salmonella seroprevalence. Fourteen pens of 8-10 pigs/pen were randomly assigned to a control (finisher diet without additive) or a treatment group (the same diet with 4kg/t of FormaXOL™) for 28 days...
February 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28107792/noteworthy-literature-published-in-2016-for-abdominal-organ-transplant-anesthesiologists
#17
Jeron Zerillo, Sang Kim, Bryan Hill, Samuel DeMaria, Tetsuro Sakai
More than 400 peer-reviewed publications on the topic of pancreas transplantation, more than 400 on intestine transplantation, and more than 3000 on renal transplantation were published in 2016. This review will highlight the most pertinent literature for anesthesiologists caring for patients undergoing non-liver abdominal organ transplantation. This review is the second part in an annual series to review relevant contributions in the field of abdominal organ transplantation focusing on pancreas, intestine, and renal transplantation...
January 1, 2017: Seminars in Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28107700/comparative-pharmacokinetics-of-eight-major-bioactive-components-in-normal-and-bacterial-diarrhea-mini-pigs-after-oral-administration-of-gegen-qinlian-decoction
#18
Xiao Ling, Yuqiang Xiang, Qingfa Tang, Feilong Chen, Xiaomei Tan
Healthy animals are most widely used in current pharmacokinetic(PK) studies. However, neglecting the effects of specific diseases on drug absorption results in the PK parameters of those experiments not accurately reflecting in vivo drug concentration changes during treatment. In this study, an E. coli infective diarrheal minipig model was applied to explore the pharmacokinetics of Gegen Qinlian decoction (GQD). A simple and rapid ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to determine the concentrations of the eight GQD components in minipig plasma after intragastric administration of GQD...
January 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28107568/oral-immunoglobulin-treatment-improved-intestinal-permeability-in-children-with-active-crohn-s-disease
#19
Tommy Sundqvist, Lars Stenhammar, Bo Tjellström, Karl-Eric Magnusson, Tony Forslund, Lotta Högberg
AIM: Crohn's disease (CD) is a chronic mucosal inflammation that affects the intestinal barrier function, for example by altering the intestinal permeability. This pilot clinical study investigated the impact of oral human immunoglobulin (OHIG) treatment on permeability characteristics in children with active luminal Crohn's disease. METHODS: The study was performed at the Department of Paediatrics, Norrköping Hospital, Sweden. Intestinal permeability was studied in three boys aged 13, 15 and 18 years with active CD, before and after a six-week treatment programme with OHIG, using different-sized polyethylene glycols the test molecules...
January 20, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28107527/effects-of-the-administration-of-25-oh-vitamin-d3-in-an-experimental-model-of-chronic-kidney-disease-in-animals-null-for-1-alpha-hydroxylase
#20
Noelia Torremadé, Milica Bozic, David Goltzman, Elvira Fernandez, José M Valdivielso
The final step in vitamin D activation is catalyzed by 1-alpha-hydroxylase (CYP27B1). Chronic kidney disease (CKD) is characterized by low levels of both 25(OH)D3 and 1,25(OH)2D3 provoking secondary hyperparathyroidism (2HPT). Therefore, treatments with active or native vitamin D compounds are common in CKD to restore 25(OH)D3 levels and also to decrease PTH. This study evaluates the dose of 25(OH)D3 that restores parathyroid hormone (PTH) and calcium levels in a model of CKD in CYP27B1-/- mice. Furthermore, we compare the safety and efficacy of the same dose in CYP27B1+/+ animals...
2017: PloS One
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