keyword
MENU ▼
Read by QxMD icon Read
search

Bdellovibrio

keyword
https://www.readbyqxmd.com/read/28510364/lyophilization-of-bdellovibrio-bacteriovorus-109j-for-long-term-storage
#1
Mélanie J Boileau, Rinosh Mani, Kenneth D Clinkenbeard
Bdellovibrio bacteriovorus 109J is a Gram-negative predatory bacterium with obligate host dependency on other Gram-negative bacteria. This bacteriolytic predator collides with, enters, and establishes growth within the prey (host) periplasm, eventually lysing the prey cell wall to release fresh, motile B. bacteriovorus progeny. Laboratory maintenance of B. bacteriovorus has been previously described by other investigators. The protocols included in this unit deal with the technique required to lyophilize or freeze dry host-dependent B...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28500337/examining-the-efficacy-of-intravenous-administration-of-predatory-bacteria-in-rats
#2
Kenneth Shatzkes, Eric Singleton, Chi Tang, Michael Zuena, Sean Shukla, Shilpi Gupta, Sonal Dharani, Joseph Rinaggio, Daniel E Kadouri, Nancy D Connell
The proteobacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are obligate predators of Gram-negative bacteria, and have been proposed to be used to treat multidrug-resistant bacterial infections. The ability of predatory bacteria to reduce bacterial burden in vivo within the lungs of rats has been demonstrated, but it was unknown if predatory bacteria can attenuate systemic bacterial burden administered intravenously. In this study, we first assessed the safety of intravenous inoculation of predatory bacteria in rats...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28492178/isolation-of-bdellovibrio-and-like-organisms-and-potential-to-reduce-acute-hepatopancreatic-necrosis-disease-caused-by-vibrio-parahaemolyticus
#3
Jetnapang Kongrueng, Pimonsri Mitraparp-Arthorn, Khotchawan Bangpanwimon, William Robins, V Vuddhakul, John Mekalanos
Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdellovibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed...
May 11, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28444379/killing-the-killer-predation-between-protists-and-predatory-bacteria
#4
Julia Johnke, Jens Boenigk, Hauke Harms, Antonis Chatzinotas
Predation by microbes is one of the main drivers of bacterial mortality in the environment. In most ecosystems multiple micropredators compete at least partially for the same bacterial resource. Predatory interactions between these micropredators might lead to shifts within microbial communities. Integrating these interactions is therefore crucial for the understanding of ecosystem functioning. In this study, we investigated the predation between two groups of micropredators, i.e. phagotrophic protists and Bdellovibrio and like organisms (BALOs)...
May 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28432347/identification-and-characterization-of-differentially-regulated-type-ivb-pilin-genes-necessary-for-predation-in-obligate-bacterial-predators
#5
Ofir Avidan, Margarita Petrenko, René Becker, Sebastian Beck, Michael Linscheid, Shmuel Pietrokovski, Edouard Jurkevitch
Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still poorly detailed. By combining omics data available for Bdellovibrio and like organisms (BALO's), we identified 43 genes expressed in B. bacteriovorus during the early interaction with prey. These included genes in a tight adherence (TAD) operon encoding for two type IVb fimbriae-like pilin proteins (flp1 and flp2), and their processing and export machinery...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28367880/predation-efficacy-of-bdellovibrio-bacteriovorus-on-multidrug-resistant-clinical-pathogens-and-their-corresponding-biofilms
#6
Yao Sun, Jianzhong Ye, Yuanbo Hou, Huale Chen, Jianming Cao, Tieli Zhou
The aim of the present study was to evaluate the predation efficacy of Bdellovibrio bacteriovorus on multidrug-resistant (MDR) or extensive drug resistant (XDR) gram-negative pathogens and their corresponding biofilms. In this study, we examined the ability of B. bacteriovorus to prey on MDR and XDR gram-negative clinical bacteria, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii. Results showed that B. bacteriovorus was able to prey on all planktonic cultures, among which the most efficient predation was found for drug-resistant E...
March 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28355554/hydrodynamic-hunters
#7
Hossein Jashnsaz, Mohammed Al Juboori, Corey Weistuch, Nicholas Miller, Tyler Nguyen, Viktoria Meyerhoff, Bryan McCoy, Stephanie Perkins, Ross Wallgren, Bruce D Ray, Konstantinos Tsekouras, Gregory G Anderson, Steve Pressé
The Gram-negative Bdellovibrio bacteriovorus (BV) is a model bacterial predator that hunts other bacteria and may serve as a living antibiotic. Despite over 50 years since its discovery, it is suggested that BV probably collides into its prey at random. It remains unclear to what degree, if any, BV uses chemical cues to target its prey. The targeted search problem by the predator for its prey in three dimensions is a difficult problem: it requires the predator to sensitively detect prey and forecast its mobile prey's future position on the basis of previously detected signal...
March 28, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28334102/predation-by-bdellovibrio-bacteriovorus-significantly-reduces-viability-and-alters-the-microbial-community-composition-of-activated-sludge-flocs-and-granules
#8
Shugeng Feng, Chuan Hao Tan, Florentin Constancias, Gurjeet S Kohli, Yehuda Cohen, Scott A Rice
We recently isolated and characterised a predatory Bdellovibrio bacteriovorus strain from activated sludge (Ulu Pandan Water Reclamation Plant, Singapore), and this strain, B. bacteriovorus UP, was able to prey upon a broad spectrum of bacterial isolates from the activated sludge when grown as planktonic cells or as biofilms. Here, we have tested the effect of Bdellovibrio predation on floccular and granular sludge to determine if the spatial organisation, loosely or tightly aggregated communities, was protective from predation...
April 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28262674/effect-of-predatory-bacteria-on-the-gut-bacterial-microbiota-in-rats
#9
Kenneth Shatzkes, Chi Tang, Eric Singleton, Sean Shukla, Michael Zuena, Shilpi Gupta, Sonal Dharani, Joseph Rinaggio, Nancy D Connell, Daniel E Kadouri
Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are Gram-negative proteobacteria that are obligate predators of other Gram-negative bacteria and are considered potential alternatives to antibiotics. Most studies focusing on predatory bacteria have been performed in vitro, thus the effect of predatory bacteria on a live host, including the impact on the ecology of the native microbiota, has yet to be fully examined. In this study, intrarectal inoculations of Sprague-Dawley rats with predatory bacteria were performed...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28177793/biocontrol-of-burkholderia-cepacia-complex-bacteria-and-bacterial-phytopathogens-by-bdellovibrio-bacteriovorus
#10
Damian McNeely, Ryan M Chanyi, James S Dooley, John E Moore, Susan F Koval
Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing...
April 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28053112/modelling-and-parameter-inference-of-predator-prey-dynamics-in-heterogeneous-environments-using-the-direct-integral-approach
#11
Itai Dattner, Ezer Miller, Margarita Petrenko, Daniel E Kadouri, Edouard Jurkevitch, Amit Huppert
Most bacterial habitats are topographically complex in the micro scale. Important examples include the gastrointestinal and tracheal tracts, and the soil. Although there are myriad theoretical studies that explore the role of spatial structures on antagonistic interactions (predation, competition) among animals, there are many fewer experimental studies that have explored, validated and quantified their predictions. In this study, we experimentally monitored the temporal dynamic of the predatory bacterium Bdellovibrio bacteriovorus, and its prey, the bacterium Burkholderia stabilis in a structured habitat consisting of sand under various regimes of wetness...
January 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27965633/initiation-of-chromosomal-replication-in-predatory-bacterium-bdellovibrio-bacteriovorus
#12
Łukasz Makowski, Rafał Donczew, Christoph Weigel, Anna Zawilak-Pawlik, Jolanta Zakrzewska-Czerwińska
Bdellovibrio bacteriovorus is a small Gram-negative predatory bacterium that attacks other Gram-negative bacteria, including many animal, human, and plant pathogens. This bacterium exhibits a peculiar biphasic life cycle during which two different types of cells are produced: non-replicating highly motile cells (the free-living phase) and replicating cells (the intracellular-growth phase). The process of chromosomal replication in B. bacteriovorus must therefore be temporally and spatially regulated to ensure that it is coordinated with cell differentiation and cell cycle progression...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27940543/bspk-a-serine-protease-from-the-predatory-bacterium-bdellovibrio-bacteriovorus-with-utility-for-analysis-of-therapeutic-antibodies
#13
Eleni Bratanis, Henrik Molina, Andreas Naegeli, Mattias Collin, Rolf Lood
The development of therapeutic and diagnostic antibodies is a rapidly growing field of research, being the fastest expanding group of products on the pharmaceutical market, and appropriate quality controls are crucial for their application. We have identified and characterized the serine protease termed BspK (Bdellovibrio serine protease K) from Bdellovibrio bacteriovorus and here show its activity on antibodies. Mutation of the serine residue at position 230 rendered the protease inactive. Further investigations of BspK enzymatic characteristics revealed autoproteolytic activity, resulting in numerous cleavage products...
February 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27915281/effects-of-predation-and-dispersal-on-bacterial-abundance-and-contaminant-biodegradation
#14
Sally Otto, Hauke Harms, Lukas Y Wick
Research into the biodegradation of soil contaminants has rarely addressed the consequences of predator-prey interactions. Here, we investigated the joint effect of predation and dispersal networks on contaminant degradation by linking spatial abundances of degrader (Pseudomonas fluorescens) and predator (Bdellovibrio bacteriovorus) bacteria to the degradation of the major soil contaminant phenanthrene (PHE). We used a laboratory microcosm with a PHE passive dosing system and a glass fiber network to facilitate bacterial dispersal...
December 2, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27889262/injections-of-predatory-bacteria-work-alongside-host-immune-cells-to-treat-shigella-infection-in-zebrafish-larvae
#15
Alexandra R Willis, Christopher Moore, Maria Mazon-Moya, Sina Krokowski, Carey Lambert, Robert Till, Serge Mostowy, R Elizabeth Sockett
Bdellovibrio bacteriovorus are predatory bacteria that invade and kill a range of Gram-negative bacterial pathogens in natural environments and in vitro [1, 2]. In this study, we investigated Bdellovibrio as an injected, antibacterial treatment in vivo, using zebrafish (Danio rerio) larvae infected with an antibiotic-resistant strain of the human pathogen Shigella flexneri. When injected alone, Bdellovibrio can persist for more than 24 hr in vivo yet exert no pathogenic effects on zebrafish larvae. Bdellovibrio injection of zebrafish containing a lethal dose of Shigella promotes pathogen killing, leading to increased zebrafish survival...
December 19, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27834203/predatory-bacteria-attenuate-klebsiella-pneumoniae-burden-in-rat-lungs
#16
Kenneth Shatzkes, Eric Singleton, Chi Tang, Michael Zuena, Sean Shukla, Shilpi Gupta, Sonal Dharani, Onoyom Onyile, Joseph Rinaggio, Nancy D Connell, Daniel E Kadouri
Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are predatory bacteria that naturally-and obligately-prey on other Gram-negative bacteria, and their use has been proposed as a potential new approach to control microbial infection. The ability of predatory bacteria to prey on Gram-negative human pathogens in vitro is well documented; however, the in vivo safety and efficacy of predatory bacteria have yet to be fully assessed. In this study, we examined whether predatory bacteria can reduce bacterial burden in the lungs in an in vivo mammalian system...
November 8, 2016: MBio
https://www.readbyqxmd.com/read/27825775/analysis-of-gene-gain-and-loss-in-the-evolution-of-predatory-bacteria
#17
Nan Li, Kai Wang, Henry N Williams, Jun Sun, Changling Ding, Xiaoyun Leng, Ke Dong
Predatory bacteria are ubiquitously distributed in nature in including in aquatic environments, sewage, intestinal tracts of animals and humans, rhizophere and, soils. However, our understanding of their evolutionary history is limited. Results of recent studies have shown that acquiring novel genes is a major force driving bacterial evolution. Therefore, to gain a better understanding of the impact of gene gain and loss in the evolution of bacterial predators, this study employed comparative genomic approaches to identify core-set gene families and species-specific gene families, and model gene gain and loss events among 11 genomes that represented diverse lineages...
January 20, 2017: Gene
https://www.readbyqxmd.com/read/27824344/catch-me-if-you-can-dispersal-and-foraging-of-bdellovibrio-bacteriovorus-109j-along-mycelia
#18
Sally Otto, Estelle P Bruni, Hauke Harms, Lukas Y Wick
To cope with heterogeneous environments and resource distributions, filamentous fungi have evolved a spatially extensive growth enabling their hyphae to penetrate air-water interfaces and pass through air-filled pores. Such mycelia are also known to act as dispersal networks for the mobilisation of bacteria ('fungal highways') and connection of microbial microhabitats. Hitherto, however, nothing is known about the effect of mycelia-based dispersal on interactions between bacterial predators and their prey and concomitant effects on biomass formation...
February 2017: ISME Journal
https://www.readbyqxmd.com/read/27629536/investigating-the-responses-of-human-epithelial-cells-to-predatory-bacteria
#19
Ajay K Monnappa, Wasimul Bari, Seong Yeol Choi, Robert J Mitchell
One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27580114/efficacy-of-bdellovibrio-bacteriovorus-109j-for-the-treatment-of-dairy-calves-with-experimentally-induced-infectious-bovine-keratoconjunctivitis
#20
Mélanie J Boileau, Rinosh Mani, Melanie A Breshears, Margi Gilmour, Jared D Taylor, Kenneth D Clinkenbeard
OBJECTIVE To determine the efficacy of Bdellovibrio bacteriovorus 109J for the treatment of calves with experimentally induced infectious bovine keratoconjunctivitis (IBK). ANIMALS 12 healthy dairy calves. PROCEDURES For each calf, a grid keratotomy was performed on both eyes immediately before inoculation with Moraxella bovis hemolytic strain Epp63-300 (n = 11 calves) or nonhemolytic strain 12040577 (1 calf). For each calf inoculated with M bovis Epp63-300, the eyes were randomly assigned to receive an artificial tear solution with (treatment group) or without (control group) lyophilized B bacteriovorus 109J...
September 2016: American Journal of Veterinary Research
keyword
keyword
64593
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"