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https://www.readbyqxmd.com/read/29212036/%C3%AE-np63-inhibits-oxidative-stress-induced-cell-death-including-ferroptosis-and-cooperates-with-the-bcl-2-family-to-promote-clonogenic-survival
#1
Gary X Wang, Ho-Chou Tu, Yiyu Dong, Anders Jacobsen Skanderup, Yufeng Wang, Shugaku Takeda, Yogesh Tengarai Ganesan, Song Han, Han Liu, James J Hsieh, Emily H Cheng
The BCL-2 family proteins are central regulators of apoptosis. However, cells deficient for BAX and BAK or overexpressing BCL-2 still succumb to oxidative stress upon DNA damage or matrix detachment. Here, we show that ΔNp63α overexpression protects cells from oxidative stress induced by oxidants, DNA damage, anoikis, or ferroptosis-inducing agents. Conversely, ΔNp63α deficiency increases oxidative stress. Mechanistically, ΔNp63α orchestrates redox homeostasis through transcriptional control of glutathione biogenesis, utilization, and regeneration...
December 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/29170133/downregulation-of-bit1-expression-promotes-growth-anoikis-resistance-and-transformation-of-immortalized-human-bronchial-epithelial-cells-via-erk-activation-dependent-suppression-of-e-cadherin
#2
Xin Yao, Selena Gray, Tri Pham, Mychael Delgardo, An Nguyen, Stephen Do, Shubha Kale Ireland, Renwei Chen, Asim B Abdel-Mageed, Hector Biliran
The mitochondrial Bit1 protein exerts tumor-suppressive function in NSCLC through induction of anoikis and inhibition of EMT. Having this dual tumor suppressive effect, its downregulation in the established human lung adenocarcinoma A549 cell line resulted in potentiation of tumorigenicity and metastasis in vivo. However, the exact role of Bit1 in regulating malignant growth and transformation of human lung epithelial cells, which are origin of most forms of human lung cancers, has not been examined. To this end, we have downregulated the endogenous Bit1 expression in the immortalized non-tumorigenic human bronchial epithelial BEAS-2B cells...
November 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29163813/the-novel-zip4-regulation-and-its-role-in-ovarian-cancer
#3
Qipeng Fan, Qingchun Cai, Pengfei Li, Wenyan Wang, Jing Wang, Emily Gerry, Tian-Li Wang, Ie-Ming Shih, Kenneth P Nephew, Yan Xu
Our RNAseq analyses revealed that ZIP4 is a top gene up-regulated in more aggressive ovarian cancer cells. ZIP4's role in cancer stem cells has not been reported in any type of cancer. In addition, the role and regulation of ZIP4, a zinc transporter, have been studied in the context of extracellular zinc transporting. Factors other than zinc with ZIP4 regulatory effects are essentially unknown. ZIP4 expression and its regulation in epithelial ovarian cancer cells was assessed by immunoblotting, quantitative PCR, or immunohistochemistry staining in human ovarian tissues...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29158789/arecoline-increases-glycolysis-and-modulates-ph-regulator-expression-in-ha22t-vgh-hepatoma-cells-leading-to-increase-of-intracellular-ca-2-reactive-oxygen-species-and-anoikis
#4
Hsiao-Ling Cheng, Wen-Tsan Chang, Yu-Chen Hu, Bau-Shan Hsieh, Tzu-Ching Huang, Inn-Wen Chong, Li-Wen Huang, Kee-Lung Chang
Background: Cancer cells proliferate rapidly and are resistant to cell death, relying on aggravated glycolysis to satisfy their increased demand for energy and biosynthetic precursors. However, this process may create unfavorable microenvironments, such as increased acidity, leading to cytotoxicity. Our previous study demonstrated that arecoline induces anoikis of HA22T/VGH hepatoma cells. The present study aimed to examine if arecoline induced anoikis is related to the glycolytic pathway and explore the underlying mechanisms...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29152096/the-non-canonical-ubiquitin-activating-enzyme-uba6-suppresses-epithelial-mesenchymal-transition-of-mammary-epithelial-cells
#5
Xianpeng Liu, Limin Sun, Demirkan B Gursel, Chonghui Cheng, Sui Huang, Alfred W Rademaker, Seema A Khan, Jun Yin, Hiroaki Kiyokawa
Ubiquitination plays critical roles in the regulation of oncoproteins and tumor suppressors during carcinogenesis. The two ubiquitin activating enzymes (E1) in human genome, UBA1 and UBA6, initiate ubiquitination by ATP-dependent activation of ubiquitin. Recent evidence suggests that UBA1 and UBA6 play partially overlapped yet distinct roles in controlling the proteome. Here we demonstrate that ubiquitination pathways initiated specifically by UBA6 set a suppressive barrier against critical steps of mammary carcinogenesis such as loss of polarity, anoikis resistance and epithelial-mesenchymal transition (EMT)...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29141222/jnk-promotes-epithelial-cell-anoikis-by-transcriptional-and-post-translational-regulation-of-bh3-only-proteins
#6
Nomeda Girnius, Roger J Davis
Developmental morphogenesis, tissue injury, and oncogenic transformation can cause the detachment of epithelial cells. These cells are eliminated by a specialized form of apoptosis (anoikis). While the processes that contribute to this form of cell death have been studied, the underlying mechanisms remain unclear. Here, we tested the role of the cJUN NH2-terminal kinase (JNK) signaling pathway using murine models with compound JNK deficiency in mammary and kidney epithelial cells. These studies demonstrated that JNK is required for efficient anoikis in vitro and in vivo...
November 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/29136735/-bit1-mediates-the-malignant-behaviors-in-pancreatic-cancer-and-its-potential-clinical-significance
#7
S Huang, D Yuan, J C Guo, T P Zhang, Y P Zhao
Objective: To investigate the potential role of Bit1 in the pathogenesis of pancreatic ductal cancer cells(PDAC) and its potential clinical application value. Methods: Real-time PCR and Western blot were employed to detect the expression of Bit1 in six pancreatic cancer cells, then the tool cells were selected to further study the function of Bit1.PolyHEMA was used to monitor the suspended cell culture condition in vitro.The invasion and migration abilities of pancreatic cancer cells were detected through Transwell assay...
November 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/29108245/reversion-of-epithelial-mesenchymal-transition-by-a-novel-agent-dz-50-via-igf-binding-protein-3-in-prostate-cancer-cells
#8
Zheng Cao, Shahriar Koochekpour, Stephen E Strup, Natasha Kyprianou
Dysregulation of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor (IGF) axis has been linked to reactive stroma dynamics in prostate cancer progression. IGF binding protein-3 (IGFBP3) induction is initiated by stroma remodeling and could represent a potential therapeutic target for prostate cancer. In previous studies a lead quinazoline-based Doxazosin® derivative, DZ-50, impaired prostate tumor growth by targeting proteins involved in focal adhesion, anoikis resistance and epithelial-mesenchymal-transition (EMT)...
October 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29074139/optogenetic-control-of-focal-adhesion-kinase-signaling
#9
Maximilian Hörner, Claire Chatelle, Wignand W D Mühlhäuser, David R Stocker, Michael Coats, Wilfried Weber, Gerald Radziwill
Focal adhesion kinase (FAK) integrates signaling from integrins, growth factor receptors and mechanical stress to control cell adhesion, motility, survival and proliferation. Here, we developed a single-component, photo-activatable FAK, termed optoFAK, by using blue light-induced oligomerization of cryptochrome 2 (CRY2) to activate FAK-CRY2 fusion proteins. OptoFAK functions uncoupled from physiological stimuli and activates downstream signaling rapidly and reversibly upon blue light exposure. OptoFAK stimulates SRC creating a positive feedback loop on FAK activation, facilitating phosphorylation of paxillin and p130Cas in adherent cells...
October 23, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/29069783/tle1-inhibits-anoikis-and-promotes-tumorigenicity-in-human-lung-cancer-cells-through-zeb1-mediated-e-cadherin-repression
#10
Xin Yao, Tri Pham, Brandi Temple, Selena Gray, Cornita Cannon, Camry Hardy, Kamari Fletcher, Shubha Kale Ireland, Ahamed Hossain, Renwei Chen, Asim B Abdel-Mageed, Hector Biliran
The Transducin-like enhancer of split 1 (TLE1) corepressor protein is overexpressed in human lung tumors and is a putative lung-specific oncogene. However, the molecular mechanism underlying its oncogenic function remains to be delineated. Here, we report an important role of TLE1 in promoting lung tumorigenesis by a mechanism involving induction of anoikis resistance. Using the human lung adenocarcinoma A549 and immortalized bronchial epithelial BEAS-2B cell lines, we observed that TLE1 inhibits anoikis through transcriptional repression of E-cadherin gene...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29068322/adult-skin-derived-precursor-schwann-cell-grafts-form-growths-in-the-injured-spinal-cord-of-fischer-rats
#11
Zacnicte May, Ranjan Kumar, Tobias Führmann, Roger Tam, Katarina Vulic, Juan Forero, Ana M Lucas-Osma, Keith Fenrich, Peggy Assinck, Michael J Lee, Aaron Moulson, Molly S Shoichet, Wolfram Tetzlaff, Jeff Biernaskie, Karim Fouad
In this study, GFP+ skin-derived precursor Schwann cells (SKP-SCs) from adult rats were grafted into the injured spinal cord of immunosuppressed rats. Our goal was to improve grafted cell survival in the injured spinal cord, which is typically low. Cells were grafted in hyaluronan-methylcellulose hydrogel (HAMC) or hyaluronan-methylcellulose modified with laminin- and fibronectin-derived peptide sequences (eHAMC). The criteria for selection of hyaluronan was for its shear-thinning properties, making the hydrogel easy to inject, methylcellulose for its inverse thermal gelation, helping to keep grafted cells in situ, and fibronectin and laminin to improve cell attachment and, thus, prevent cell death due to dissociation from substrate molecules (i...
October 25, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29061507/microrna-30a-suppresses-autophagy-mediated-anoikis-resistance-and-metastasis-in-hepatocellular-carcinoma
#12
Xiu-Tao Fu, Ying-Hong Shi, Jian Zhou, Yuan-Fei Peng, Wei-Ren Liu, Guo-Ming Shi, Qiang Gao, Xiao-Ying Wang, Kang Song, Jia Fan, Zhen-Bin Ding
MiRNA-30a (miR-30a) was previously reported as one of metastatic hepatocellular carcinoma (HCC)-related microRNAs. However, the function of miR-30a on enhancing our biological understanding of HCC metastasis is not clear. This study demonstrated that miR-30a was significantly down-regulated in HCC tissues and cell lines, and was associated with vascular invasion, metastasis potential and recurrent disease in HCC. Functional studies confirmed that miR-30a could inhibit the metastasis of HCC in a well-established nude mouse model of lung metastasis...
October 20, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29038437/srvf-a-novel-herbal-formula-including-scrophulariae-radix-and-viticis-fructus-disrupts-focal-adhesion-and-causes-detachment-induced-apoptosis-in-malignant-cancer-cells
#13
Aeyung Kim, Minju Im, Jin Yeul Ma
When cells lose adhesion, they undergo detachment-induced apoptosis, known as anoikis. In contrast, tumor cells acquire resistance to anoikis, enabling them to survive, even after separating from neighboring cells or the ECM. Therefore, agents that restore anoikis sensitivity may serve as anti-cancer candidates. In this study, we constructed a novel herbal formula, SRVF, which contains Scrophulariae Radix (SR) and Viticis Fructus (VF). SRVF rapidly decreased cell adhesion, altered the cell morphology to round, and induced cell death; however, SR, VF, or their co-treatment did not...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29033534/multicellular-tumor-spheroids-of-human-uveal-melanoma-induce-genes-associated-with-anoikis-resistance-lipogenesis-and-ssxs
#14
Charlotte Ness, Øystein Garred, Nils A Eide, Theresa Kumar, Ole K Olstad, Thomas P Bærland, Goran Petrovski, Morten C Moe, Agate Noer
PURPOSE: Uveal melanoma (UM) has a high propensity for metastatic spread, and approximately 40-50% of patients die of metastatic disease. Metastases can be found at the time of diagnosis but also several years after the tumor has been removed. The survival of disseminated cancer cells is known to be linked to anchorage independence, anoikis resistance, and an adaptive cellular metabolism. The cultivation of cancer cells as multicellular tumor spheroids (MCTS) by anchorage-independent growth enriches for a more aggressive phenotype...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29029495/metadherin-promotes-metastasis-by-supporting-putative-cancer-stem-cell-properties-and-epithelial-plasticity-in-pancreatic-cancer
#15
Kensuke Suzuki, Shigetsugu Takano, Hideyuki Yoshitomi, Hitoe Nishino, Shingo Kagawa, Hiroaki Shimizu, Katsunori Furukawa, Masaru Miyazaki, Masayuki Ohtsuka
Pancreatic ductal adenocarcinoma (PDAC) has a high metastatic potential. However, the mechanism of metastatic colonization in PDAC remains poorly understood. Metadherin (MTDH) has emerged in recent years as a crucial mediator of metastasis in several cancer types, although the biological role of MTDH in PDAC has not been investigated. Here, we demonstrated the functional roles of MTDH in PDAC progression, especially focusing on the metastatic cascade. In vitro studies showed that MTDH provides cancer stem cell (CSC) properties in metastatic PDAC cells and contributes to anoikis resistance with epithelial characteristics in PDAC cells...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28993682/olaparib-modulates-dna-repair-efficiency-sensitizes-cervical-cancer-cells-to-cisplatin-and-exhibits-anti-metastatic-property
#16
Chandra Bhushan Prasad, Shyam Babu Prasad, Suresh Singh Yadav, Laxmi Kant Pandey, Sunita Singh, Satyajit Pradhan, Gopeshwar Narayan
PARP1 trapping at DNA lesion by pharmacological inhibitors has been exploited in several cancers exhibiting defects in DNA repair mechanisms. PARP1 hyperactivation is involved in therapeutic resistance in multiple cancers. The role of PARP1 in cervical cancer (CC) resistance and implication of PARP inhibitor is yet to be elucidated. Our data demonstrates significantly higher expression of PARP1 in primary cervical tumors and CC cell lines SiHa and ME180. Upon cisplatin treatment CC cells display significant overexpression of PARP1 and its hyperactivation...
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28990814/induction-of-anoikis-in-human-colorectal-cancer-cells-by-fucoxanthinol
#17
Masaru Terasaki, Hayato Maeda, Kazuo Miyashita, Michihiro Mutoh
Fucoxanthin (Fx), one of the major xanthophylls in brown algae, is known to be effective for colorectal cancer (CRC) chemoprevention through inhibiting cell growth, cell cycle and caspase activation. Recently, we observed fucoxanthinol (FuOH), an anti-cancer active metabolite of Fx, treatment of human CRC cells resulted in plenty of living floating cells several hours after exposure, and induced apoptosis. In the present study, we investigated whether FuOH induced anchorage-dependent apoptosis, that is "anoikis", along with integrin signal suppression in human CRC cells...
October 9, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28988620/collapsing-the-metabolic-pon2zi-scheme-in-pancreatic-ductal-adenocarcinoma
#18
Matyas Abel Tsegaye, Zachary T Schafer
A hallmark of pancreatic ductal adenocarcinoma cancer (PDAC) cells is metabolic reprogramming that facilitates tumor progression. In a recent paper published in Molecular Cell, Nagarajan et al. discover that paraoxonase (PON)2 stimulates glucose transporter (GLUT)1-mediated glucose uptake, prevents AMP-activated protein kinase (AMPK)-mediated anoikis, and consequently promotes PDAC development and metastasis.
November 2017: Trends in Cell Biology
https://www.readbyqxmd.com/read/28988392/a-guide-for-endometrial-cancer-cell-lines-functional-assays-using-the-measurements-of-electronic-impedance
#19
Joanna Kozak, Paulina Wdowiak, Ryszard Maciejewski, Anna Torres
Endometrial cancer cell lines are critical tools to investigate the molecular mechanism of tumorigenesis using the end point cell-based assay such as proliferation, cytotoxicity, apoptosis, anoikis or migration and invasion. The proper assay optimization and performance is essential for physiologically relevant results interpretation. In this study we use label-free real-time cell analysis platform (xCELLigence) to optimize growing conditions for proliferation and migration experiments of two types of endometrial cancer cell lines HEC-1-B, HEC-1-A, KLE, and Ishikawa...
October 7, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28977882/enhanced-chemosensitization-of-anoikis-resistant-melanoma-cells-through-syndecan-2-upregulation-upon-anchorage-independency
#20
TingTing Tseng, WuChing Uen, JenChih Tseng, ShaoChen Lee
Syndecan family proteins are heparan sulfate proteoglycans, which involved in various cellular activities and associating with metastatic potential and chemosensitivity of tumor cells. Melanoma is one of malignant tumors with poor prognosis upon metastasis. Previously, we had shown that melanoma cells remained survived under cell detachment, which was similar to the initial steps of tumor metastasis. Downregulation of syndecan-1 and upregulation of syndecan-2 in melanoma A375 cells were observed by different suspension conditions...
September 22, 2017: Oncotarget
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