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https://www.readbyqxmd.com/read/28535600/new-approach-to-resolve-the-humidity-problem-in-voc-determination-in-outdoor-air-samples-using-solid-adsorbent-tubes-followed-by-td-gc-ms
#1
Alba Maceira, Laura Vallecillos, Francesc Borrull, Rosa Maria Marcé
This study describes the humidity effect in the sampling process by adsorbent tubes followed by thermal desorption and gas chromatography-mass spectrometry (TD-GC-MS) for the determination of volatile organic compounds (VOCs) in air samples and evaluates possible solutions to this problem. Two multi-sorbent bed tubes, Tenax TA/Carbograph 1TD and Carbotrap B/Carbopack X/Carboxen 569, were tested in order to evaluate their behaviour in the presence of environmental humidity. Humidity problems were demonstrated with carbon-based tubes, while Tenax-based tubes did not display any influence...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535580/gastroparesis-updates-on-pathogenesis-and-management
#2
REVIEW
Nanlong Liu, Thomas Abell
Gastroparesis (Gp) is a chronic disease that presents with clinical symptoms of early satiety, bloating, nausea, vomiting, and abdominal pain. Along with these symptoms, an objective finding of delayed gastric emptying, along with a documented absence of gastric outlet obstruction, are required for diagnosis. This article focuses on updates in the pathogenesis and management of Gp. Recent studies on full thickness biopsies of Gp patients have shed light on the complex interactions of the central, autonomic, and enteric nervous systems, which all play key roles in maintaining normal gut motility...
May 25, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28535467/molecular-approaches-to-the-diagnosis-and-monitoring-of-production-diseases-in-pigs
#3
REVIEW
Timothy A Giles, Aouatif Belkhiri, Paul A Barrow, Neil Foster
Production disease in pigs is caused by a variety of different pathogens, mainly enteric and respiratory and can result in significant economic loss. Other factors such as stress, poor husbandry and nutrition can also contribute to an animal's susceptibility to disease. Molecular biomarkers of production disease could be of immense value by improving diagnosis and risk analysis to determine best practice with an impact on increased economic output and animal welfare. In addition to the use of multiplex PCR or microarrays to detect individual or mixed pathogens during infection, these technologies can also be used to monitor the host response to infection via gene expression...
May 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28535265/physiology-of-cold-hardiness-seasonal-fluctuations-and-cryoprotectant-contents-in-overwintering-adults-of-hypera-postica-coleoptera-curculionidae
#4
Mahdieh Saeidi, Saeid Moharramipour
The alfalfa weevil, Hypera postica (Gyllenhal), is widely distributed in various alfalfa-growing regions in the world. Adults enter the field gradually from hibernation places and overwinter in a nondiapausing state, exhibiting feeding, mating, and oviposition over autumn and winter whenever the weather permits. In this study, the variability of supercooling point (SCP) and cold hardiness in alfalfa weevil was investigated. Adults were collected monthly from alfalfa fields in Karaj, Iran, during autumn, winter, and spring in 2011-2012...
May 23, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28535197/feeding-and-development-of-emerald-ash-borer-coleoptera-buprestidae-on-cultivated-olive-olea-europaea
#5
Don Cipollini, Chad M Rigsby, Donnie L Peterson
We examined the suitability of cultivated olive, Olea europaea L., as a host for emerald ash borer, Agrilus planipennis Fairmaire. In a bioassay using cut stems from a field-grown olive tree (cv. 'Manzanilla') we found that 45% of larvae that had emerged from eggs used to inoculate stems, were recovered alive, many as larvae or prepupae, during periodic debarking of a subset of stems. Three intact stems that 19 larvae successfully entered were exposed to a simulated overwintering treatment. Four live adults emerged afterwards, and an additional pupa and several prepupae were discovered after debarking these stems...
May 23, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28534720/spatial-and-visual-reasoning-do-these-abilities-improve-in-first-year-veterinary-medical-students-exposed-to-an-integrated-curriculum
#6
J Claudio Gutierrez, Munashe Chigerwe, Jan E Ilkiw, Patricia Youngblood, Steven D Holladay, Sakti Srivastava
Spatial visualization ability refers to the human cognitive ability to form, retrieve, and manipulate mental models of spatial nature. Visual reasoning ability has been linked to spatial ability. There is currently limited information about how entry-level spatial and visual reasoning abilities may predict veterinary anatomy performance or may be enhanced with progression through the veterinary anatomy content in an integrated curriculum. The present study made use of two tests that measure spatial ability, and one test that measures visual reasoning ability in veterinary students: Guay's Visualization of Views Test, adapted version (GVVT), the Mental Rotations Test (MRT), and Raven's Advanced Progressive Matrices Test, short form (RavenT)...
May 23, 2017: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/28534615/origins-of-water-molecules-in-the-photosystem-ii-crystal-structure
#7
Naoki Sakashita, Hiroshi C Watanabe, Takuya Ikeda, Keisuke Saito, Hiroshi Ishikita
The cyanobacterial photosystem II (PSII) crystal structure includes more than 1300 water molecules in each monomer unit; however, their precise roles in water oxidation are unclear. To understand the origins of water molecules in the PSII crystal structure, the accessibility of bulk water molecules to channel inner spaces in PSII was investigated using the water-removed PSII structure and molecular dynamics (MD) simulations. The inner space of the channel that proceeds toward the D1-Glu65/D2-Glu312 pair (E65/E312 channel) was entirely filled with water molecules from the bulk region...
May 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28534613/flashbody-a-next-generation-fluobody-with-fluorescence-intensity-enhanced-by-antigen-binding
#8
Devina Wongso, Jinhua Dong, Hiroshi Ueda, Tetsuya Kitaguchi
Fluorescent probes are valuable tools for visualizing the spatiotemporal dynamics of molecules in living cells. Here we developed a genetically-encoded antibody probe with antigen-dependent fluorescence intensity called "Flashbody". We first created a fusion of EGFP to the single chain variable region fragment (scFv) of antibody against seven amino acids of the bone Gla protein C-terminus (BGPC7) called BGP Fluobody, which successfully showed the intracellular localization of BGPC7-tagged protein. To generate BGP Flashbody, circularly permuted GFP was inserted in between two variable region fragments, and the linkers were optimized, resulting in fluorescence intensity increase of 300% upon binding with BGPC7 in a dose-dependent manner...
May 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28534606/standardized-reporting-of-functioning-information-on-icf-based-common-metrics
#9
Birgit Prodinger, Alan Tennant, Gerold Stucki
BACKGROUND: In clinical practice and research a variety of clinical data collection tools are used to collect information on people's functioning for clinical practise and research and National Health Information Systems. Reporting on ICF-based common metrics enables standardized documentation of functioning information in national health information systems. OBJECTIVE: The objective of this methodological note on applying the ICF in rehabilitation is to demonstrate how to report functioning information collected with a data collection tool on ICF-based common metrics...
May 23, 2017: European Journal of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/28534417/clinical-and-cost-effectiveness-of-home-based-cardiac-rehabilitation-compared-to-conventional-centre-based-cardiac-rehabilitation-results-of-the-fit-home-study
#10
Jos J Kraal, M Elske Van den Akker-Van Marle, Ameen Abu-Hanna, Wim Stut, Niels Peek, Hareld Mc Kemps
Aim Although cardiac rehabilitation improves physical fitness after a cardiac event, many eligible patients do not participate in cardiac rehabilitation and the beneficial effects of cardiac rehabilitation are often not maintained over time. Home-based training with telemonitoring guidance could improve participation rates and enhance long-term effectiveness. Methods and results We randomised 90 low-to-moderate cardiac risk patients entering cardiac rehabilitation to three months of either home-based training with telemonitoring guidance or centre-based training...
January 1, 2017: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/28534331/-application-of-bowel-plication-combined-with-early-enteral-nutrition-in-the-enhanced-recovery-after-surgery-for-neonates-with-jejunal-atresia
#11
Huan Chen, Qiming Geng, Changgui Lu, Weiwei Jiang, Jie Zhang, Xiaofeng Lyu, Wei Li, Hongxing Li, Weibing Tang
OBJECTIVE: To evaluate the efficacy of bowel plication combined with early enteral nutrition (EEN) in the enhanced recovery after surgery(ERAS) of jejunal atresia (JA) neonates. METHODS: Between January 2005 and January 2014, 58 neonates with JA underwent surgical treatment in Children's Hospital of Nanjing Medical University. Their clinical data, including operation procedures, ages, birth weight, concomitant diseases, age at surgery, hospital stay, total parenteral nutrition (TPN), postoperative intestinal function recovery (the time to the first oral feeding and the time to oral feeding volume reaching 150 ml·kg(-1)·d(-1)), complications and reoperation, were retrospectively analyzed...
May 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28534323/-era-of-enhanced-recovery-after-surgery-and-robotic-gastric-cancer-surgery
#12
Yanbing Zhou
Enhanced recovery after surgery (ERAS) has been rapidly developing by combining several techniques with evidence-based adjustments, including preoperative education, preoperative carbohydrate loading, epidural or regional anesthesia, early initiation of enteral nutrition, ambulation and multi-modal pain management. The core part of ERAS is to reduce and reverse surgical stress and therefore greatly improve clinical outcome. Under the guidance of ERAS, perioperative management of robotic gastric cancer operation should follow the basic principles of ERAS and clinical pathway to maximize the advantages of the robotic surgery...
May 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28534056/carbon-nanomaterials-enhance-survival-of-agapanthus-praecox-callus-after-cryopreservation-by-vitrification
#13
S M Chen, L Ren, D Zhang, Y F Zhang, X H Shen
BACKGROUND: Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility. OBJECTIVE: In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed. MATERIALS AND METHODS: The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534028/mechanisms-affecting-the-gut-of-preterm-infants-in-enteral-feeding-trials
#14
Nicholas D Embleton, Janet E Berrington, Jon Dorling, Andrew K Ewer, Edmund Juszczak, John A Kirby, Christopher A Lamb, Clare V Lanyon, William McGuire, Christopher S Probert, Stephen P Rushton, Mark D Shirley, Christopher J Stewart, Stephen P Cummings
Large randomized controlled trials (RCTs) in preterm infants offer unique opportunities for mechanistic evaluation of the risk factors leading to serious diseases, as well as the actions of interventions designed to prevent them. Necrotizing enterocolitis (NEC) a serious inflammatory gut condition and late-onset sepsis (LOS) are common feeding and nutrition-related problems that may cause death or serious long-term morbidity and are key outcomes in two current UK National Institutes for Health Research (NIHR) trials...
2017: Frontiers in Nutrition
https://www.readbyqxmd.com/read/28533818/a-novel-bcr-abl1-fusion-gene-with-genetic-heterogeneity-indicates-a-good-prognosis-in-a-chronic-myeloid-leukemia-case
#15
Fen Zhou, Runming Jin, Yu Hu, Heng Mei
BACKGROUND: Chronic myelogenous leukemia (CML) is a pluripotent hematopoietic stem cell disorder caused by the fusion of the BCR and ABL1 genes. Quantitative RT-PCR (qRT-PCR) is a routinely performed screening technique to identify BCR-ABL1 fusion genes, but a limitation of this method is its inability to recognize novel fusions that have not been previously characterized. Next-generation sequencing (NGS) is an effective and sensitive detection method for the determination of novel BCR-ABL1 fusion genes as well as previously characterized ones...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/28533783/progress-on-azadirachta-indica-based-biopesticides-in-replacing-synthetic-toxic-pesticides
#16
REVIEW
Suman Chaudhary, Rupinder K Kanwar, Alka Sehgal, David M Cahill, Colin J Barrow, Rakesh Sehgal, Jagat R Kanwar
Over the years, extensive use of commercially available synthetic pesticides against phytophagous insects has led to their bioaccumulation in the environment causing increased resistance and reduction in soil biodiversity. Further, 90% of the applied pesticides enter the various environmental resources as a result of run-off, exposing the farmers as well as consumers of the agricultural produce to severe health issues. Therefore, growing attention has been given toward the development of alternate environmentally friendly pesticides/insecticides that would aid an efficient pest management system and also prevent chronic exposures leading to diseases...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28533442/the-role-of-shed-prp-c-in-the-neuropathogenesis-of-hiv-infection
#17
Bezawit W Megra, Eliseo A Eugenin, Joan W Berman
HIV-1 enters the CNS soon after peripheral infection and causes chronic neuroinflammation and neuronal damage that leads to cognitive impairment in 40-70% of HIV-infected people. The nonpathogenic cellular isoform of the human prion protein (PrP(c)) is an adhesion molecule constitutively expressed in the CNS. Previously, our laboratory showed that shed PrP(c) (sPrP(c)) is increased in the cerebrospinal fluid of HIV-infected people with cognitive deficits as compared with infected people with no impairment. In this article, we demonstrate that CCL2 and TNF-α, inflammatory mediators that are elevated in the CNS of HIV-infected people, increase shedding of PrP(c) from human astrocytes by increasing the active form of the metalloprotease ADAM10...
May 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28533362/cgas-is-essential-for-cellular-senescence
#18
Hui Yang, Hanze Wang, Junyao Ren, Qi Chen, Zhijian J Chen
Cellular senescence is a natural barrier to tumorigenesis and it contributes to the antitumor effects of several therapies, including radiation and chemotherapeutic drugs. Senescence also plays an important role in aging, fibrosis, and tissue repair. The DNA damage response is a key event leading to senescence, which is characterized by the senescence-associated secretory phenotype (SASP) that includes expression of inflammatory cytokines. Here we show that cGMP-AMP (cGAMP) synthase (cGAS), a cytosolic DNA sensor that activates innate immunity, is essential for senescence...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28533183/dedicated-research-time-during-surgery-residency-leads-to-a-significant-decline-in-self-assessed-clinical-aptitude-and-surgical-skills
#19
Monica M Grova, Anthony D Yang, Misty D Humphries, Joseph M Galante, Edgardo S Salcedo
OBJECTIVE: The surgical community commonly perceives a decline in surgical and patient care skills among residents who take dedicated time away from clinical activity to engage in research. We hypothesize that residents perceive a decline in their skills because of dedicated research time. SETTING: UC Davis Medical Center, Sacramento, CA, an institutional tertiary care center. PARTICIPANTS: General surgery residents and graduates from UC Davis general surgery residency training program, who had completed at least 1 year of research during their training...
May 19, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28532994/johne-s-disease-in-the-eyes-of-irish-cattle-farmers-a-qualitative-narrative-research-approach-to-understanding-implications-for-disease-management
#20
Conor G McAloon, Áine Macken-Walsh, Lisa Moran, Paul Whyte, Simon J More, Luke O'Grady, Michael L Doherty
Bovine Johne's Disease (JD) is a disease characterised by chronic granulomatous enteritis which manifests clinically as a protein-losing enteropathy causing diarrhoea, hypoproteinaemia, emaciation and, eventually death. Some research exists to suggest that the aetiologic pathogen Mycobacterium avium subspecies paratuberculosis may pose a zoonotic risk. Nationally coordinated control programmes have been introduced in many of the major milk producing countries across the world. However, JD is challenging to control in infected herds owing to limitations of diagnostic tests and the long incubation period of the disease...
June 1, 2017: Preventive Veterinary Medicine
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