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bone marrow perfusion

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https://www.readbyqxmd.com/read/29193065/stereological-assessment-of-the-blood-air-barrier-and-the-surfactant-system-after-mesenchymal-stem-cell-pretreatment-in-a-porcine-non-heart-beating-donor-model-for-lung-transplantation
#1
Anke Schnapper, Astrid Christmann, Lars Knudsen, Parwis Rahmanian, Yeong-Hoon Choi, Mohamed Zeriouh, Samira Karavidic, Klaus Neef, Anja Sterner-Kock, Maria Guschlbauer, Florian Hofmaier, Alexandra C Maul, Thorsten Wittwer, Thorsten Wahlers, Christian Mühlfeld, Matthias Ochs
More frequent utilization of non-heart-beating donor (NHBD) organs for lung transplantation has the potential to relieve the shortage of donor organs. In particular with respect to uncontrolled NHBD, concerns exist regarding the risk of ischaemia/reperfusion (IR) injury-related graft damage or dysfunction. Due to their immunomodulating and tissue-remodelling properties, bone-marrow-derived mesenchymal stem cells (MSCs) have been suspected of playing a beneficial role regarding short- and long-term survival and function of the allograft...
November 28, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/29179177/permanently-hypoxic-cell-culture-yields-rat-bone-marrow-mesenchymal-cells-with-higher-therapeutic-potential-in-the-treatment-of-chronic-myocardial-infarction
#2
Yihua Liu, Xiaoxi Yang, Pablo Maureira, Aude Falanga, Vanessa Marie, Guillaume Gauchotte, Sylvain Poussier, Frederique Groubatch, Pierre-Yves Marie, Nguyen Tran
BACKGROUND: The mismatch between traditional in vitro cell culture conditions and targeted chronic hypoxic myocardial tissue could potentially hamper the therapeutic effects of implanted bone marrow mesenchymal stem cells (BMSCs). This study sought to address (i) the extent of change to BMSC biological characteristics in different in vitro culture conditions and (ii) the effectiveness of permanent hypoxic culture for cell therapy in treating chronic myocardial infarction (MI) in rats...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29177236/a-new-strategy-and-system-for-the-ex-vivo-ovary-perfusion-and-cryopreservation-an-innovation
#3
Mohamed Shehata Ali Mohamed
Children and young adults, who suffer from cancer, receive gonadotoxic therapy, which destroys their fertile abilities after survival. Ovarian cryopreservation and transplantation provide the promising solution to this problem, where the ovary can be removed before the gonadotoxic therapy and reimplanted after patient's survival, where the ovary is to be cryopreserved during the period of the therapy. However, cryopreservation of the whole ovary is still facing great obstacles, namely the ischemic reperfusion injury and the defective cryopreservation related to the defective ability to universally deliver the cryopreservation/warming solutions through the ovarian vascular bed...
June 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29137612/comparison-of-semi-quantitative-and-quantitative-dynamic-contrast-enhanced-mri-evaluations-of-vertebral-marrow-perfusion-in-a-rat-osteoporosis-model
#4
Jingqi Zhu, Zuogang Xiong, Jiulong Zhang, Yuyou Qiu, Ting Hua, Guangyu Tang
BACKGROUND: This study aims to investigate the technical feasibility of semi-quantitative and quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the assessment of longitudinal changes of marrow perfusion in a rat osteoporosis model, using bone mineral density (BMD) measured by micro-computed tomography (micro-CT) and histopathology as the gold standards. METHODS: Fifty rats were randomly assigned to the control group (n=25) and ovariectomy (OVX) group whose bilateral ovaries were excised (n=25)...
November 14, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29121817/stromal-cell-derived-factor-1%C3%AE-promotes-endothelial-colony-forming-cell-migration-through-the-ca2-dependent-activation-of-the-extracellular-signal-regulated-kinase-1-2-and-phosphoinositide-3-kinase-akt-pathways
#5
Estella Zuccolo, Christian Andrea Di Buduo, Francesco Lodola, Stefania Orecchioni, Giorgia Scarpellino, Dlzar Ali Kheder, Valentina Poletto, Germano Guerra, Francesco Bertolini, Alessandra Balduini, Vittorio Rosti, Francesco Moccia
Stromal derived factor-1α (SDF-1α) drives endothelial colony forming cell (ECFC) homing and incorporation within neovessels, thereby restoring tissue perfusion in ischemic tissues and favouring tumor vascularization and metastasis. SDF-1α stimulates ECFC migration by activating the Gi-protein coupled receptor, CXCR4, and then engaging the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway. Sporadic evidence showed that SDF-1α may also act through an increase in intracellular Ca2+ concentration ([Ca2+]i) in bone marrow-derived hematopoietic progenitor cells and fully differentiated endothelial cells...
November 9, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29103037/circulating-progenitor-cells-correlate-with-memory-posterior-cortical-thickness-and-hippocampal-perfusion
#6
Daniel A Nation, Alick Tan, Shubir Dutt, Elissa C McIntosh, Belinda Yew, Jean K Ho, Anna E Blanken, Jung Yun Jang, Kathleen E Rodgers, Aimée Gaubert
BACKGROUND: Bone marrow-derived progenitor cells survey the vasculature and home to sites of tissue injury where they can promote repair and regeneration. It has been hypothesized that these cells may play a protective role neurodegenerative and vascular cognitive impairment. OBJECTIVE: To evaluate progenitor cell levels in older adults with and without mild cognitive impairment (MCI), and to relate circulating levels to memory, brain volume, white matter lesion volume, and cerebral perfusion...
November 1, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29102342/bioengineered-lungs-a-challenge-and-an-opportunity
#7
Ramon Farré, Jordi Otero, Isaac Almendros, Daniel Navajas
Lung biofabrication is a new tissue engineering and regenerative development aimed at providing organs for potential use in transplantation. Lung biofabrication is based on seeding cells into an acellular organ scaffold and on culturing them in an especial purpose bioreactor. The acellular lung scaffold is obtained by decellularizing a non-transplantable donor lung by means of conventional procedures based on application of physical, enzymatic and detergent agents. To avoid immune recipient's rejection of the transplanted bioengineered lung, autologous bone marrow/adipose tissue-derived mesenchymal stem cells, lung progenitor cells or induced pluripotent stem cells are used for biofabricating the bioengineered lung...
November 1, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29096622/tissue-engineered-smooth-muscle-cell-and-endothelial-progenitor-cell-bi-level-cell-sheets-prevent-progression-of-cardiac-dysfunction-microvascular-dysfunction-and-interstitial-fibrosis-in-a-rodent-model-of-type-1-diabetes-induced-cardiomyopathy
#8
Masashi Kawamura, Michael J Paulsen, Andrew B Goldstone, Yasuhiro Shudo, Hanjay Wang, Amanda N Steele, Lyndsay M Stapleton, Bryan B Edwards, Anahita Eskandari, Vi N Truong, Kevin J Jaatinen, Arnar B Ingason, Shigeru Miyagawa, Yoshiki Sawa, Y Joseph Woo
BACKGROUND: Diabetes mellitus is a risk factor for coronary artery disease and diabetic cardiomyopathy, and adversely impacts outcomes following coronary artery bypass grafting. Current treatments focus on macro-revascularization and neglect the microvascular disease typical of diabetes mellitus-induced cardiomyopathy (DMCM). We hypothesized that engineered smooth muscle cell (SMC)-endothelial progenitor cell (EPC) bi-level cell sheets could improve ventricular dysfunction in DMCM. METHODS: Primary mesenchymal stem cells (MSCs) and EPCs were isolated from the bone marrow of Wistar rats, and MSCs were differentiated into SMCs by culturing on a fibronectin-coated dish...
November 2, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28978662/fractalkine-induces-angiogenic-potential-in-cx3cr1-expressing-monocytes
#9
Youngrok Park, Joon Lee, Jae-Yong Kwak, Kyoungmi Noh, Eunjung Yim, Hyun-Kyung Kim, Young June Kim, Hal E Broxmeyer, Jeong-A Kim
We report the unique role of CX3CL1 (or fractalkine) on CD11b(+) myelomonocytic cells expressing CX3CR1, the only known receptor for CX3CL1, in promoting blood perfusion recovery. In a mouse ischemic hind-limb model, CD11b(+)CX3CR1(+) cells migrated to ischemic femoral muscles through CX3CL1-mediated chemotaxis. CD11b(+)CX3CR1(+) macrophages isolated from ischemic tissues [tissue (T)-CD11b(+)CX3CR1(+)] of muscle exert a proangiogenic effect through platelet factor-4 (CXCL4; PF-4) production. PF-4 does not promote angiogenesis by itself but, instead, increases VEGF-mediated angiogenesis...
October 4, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28978523/recombinant-sclerostin-antagonises-effects-of-ex-vivo-mechanical-loading-in-trabecular-bone-and-increases-osteocyte-lacunar-size
#10
Masakazu Kogawa, Kamarul A Khalid, Asiri R Wijenayaka, Renee T Ormsby, Andreas Evdokiou, Paul H Anderson, David M Findlay, Gerald J Atkins
Sclerostin (SCL) has emerged as an important regulator of bone mass. We have shown that SCL can act by targeting late osteoblasts/osteocytes to inhibit bone mineralisation and to upregulate osteocyte expression of catabolic factors, resulting in osteocytic osteolysis. Here we sought to examine the effect of exogenous sclerostin on osteocytes in trabecular bone mechanically loaded ex vivo. Bovine trabecular bone cores, with bone marrow removed, were inserted into individual chambers and subjected to daily episodes of dynamic loading...
October 4, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28976370/-what-is-aging-and-cardiovascular-aging
#11
Z Lale Koldaş
Aging is one of the main risk factors for cardiovascular disease (CVD) and results in a progressive functional decline of organs and the vasculature. According to WHO data today, 8.5 percent of people worldwide (617 million) are aged 65 and over, this percentage is projected to jump to nearly 17 percent of the world's population by 2050 (1.6 billion). Turkey's 65-and- over population rise over the last 5 years about 17,1% and it is estimated that the percentage of this elderly population will rise from 8.5% (2016 data) to 20,8% in the year 2050...
September 2017: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
https://www.readbyqxmd.com/read/28899758/chronic-global-analysis-of-vascular-permeability-and-cerebral-blood-flow-after-bone-marrow-stromal-cell-treatment-of-traumatic-brain-injury-in-the-rat-a-long-term-mri-study
#12
Lian Li, Michael Chopp, Guangliang Ding, Qingjiang Li, Asim Mahmood, Quan Jiang
Vascular permeability and hemodynamic alteration in response to the transplantation of human bone marrow stromal cells (hMSCs) after traumatic brain injury (TBI) were longitudinally investigated in non directly injured and normal-appearing cerebral tissue using magnetic resonance imaging (MRI). Male Wistar rats (300-350g, n=30) subjected to controlled cortical impact TBI were intravenously injected with 1ml of saline (at 6-h or 1-week post-injury, n=5/group) or with hMSCs in suspension (∼3×10(6) hMSCs, at 6-h or 1-week post-injury, n=10/group)...
November 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28898758/modular-flow-chamber-for-engineering-bone-marrow-architecture-and-function
#13
Christian A Di Buduo, Paolo M Soprano, Lorenzo Tozzi, Stefania Marconi, Ferdinando Auricchio, David L Kaplan, Alessandra Balduini
The bone marrow is a soft, spongy, gelatinous tissue found in the hollow cavities of flat and long bones that support hematopoiesis in order to maintain the physiologic turnover of all blood cells. Silk fibroin, derived from Bombyx mori silkworm cocoons, is a promising biomaterial for bone marrow engineering, because of its tunable architecture and mechanical properties, the capacity of incorporating labile compounds without loss of bioactivity and demonstrated ability to support blood cell formation. In this study, we developed a bone marrow scaffold consisting of a modular flow chamber made of polydimethylsiloxane, holding a silk sponge, prepared with salt leaching methods and functionalized with extracellular matrix components...
November 2017: Biomaterials
https://www.readbyqxmd.com/read/28881093/expansion-of-bone-marrow-mesenchymal-stromal-cells-in-perfused-3d-ceramic-scaffolds-enhances-in-vivo-bone-formation
#14
Allison I Hoch, Ralph Duhr, Nunzia Di Maggio, Arne Mehrkens, Marcel Jakob, David Wendt
Bone marrow-derived mesenchymal stromal cells (BMSC), when expanded directly within 3D ceramic scaffolds in perfusion bioreactors, more reproducibly form bone when implanted in vivo as compared to conventional expansion on 2D polystyrene dishes/flasks. Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, we aimed to decouple the effects of specific parameters among these two model systems. We assessed the effects of the: 1) 3D scaffold vs...
September 7, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28830269/adhesion-proliferation-and-osteogenic-differentiation-of-human-mscs-cultured-under-perfusion-with-a-marine-oxygen-carrier-on-an-allogenic-bone-substitute
#15
Fiona Le Pape, Gaëlle Richard, Emmanuelle Porchet, Sophie Sourice, Frédéric Dubrana, Claude Férec, Valérie Polard, Richard Pace, Pierre Weiss, Franck Zal, Pascal Delépine, Elisabeth Leize
Tissue engineering strategies have been developed to optimize osseointegration in dental implant surgery. One of the major problems is the non-homogeneous spatial cell distribution in the scaffold, as well as subsequent matrix production. Insufficient nutrient and oxygen supplies inside the scaffold are factors in this phenomenon. To mediate this gradient formation, we have implemented a perfusion culture method to seed human bone marrow mesenchymal stem cells (MSCs) into three-dimensional (3-D)-allogenic bone scaffolds in combination with a marine haemoglobin, HEMOXCell(®), for oxygen delivery...
August 22, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28816358/trpc3-mediated-ca-2-signals-as-a-promising-strategy-to-boost-therapeutic-angiogenesis-in-failing-hearts-the-role-of-autologous-endothelial-colony-forming-cells
#16
REVIEW
Francesco Moccia, Angela Lucariello, Germano Guerra
Endothelial progenitor cells (EPCs) are a sub-population of bone marrow-derived mononuclear cells that are released in circulation to restore damaged endothelium during its physiological turnover or rescue blood perfusion after an ischemic insult. Additionally, they may be mobilized from perivascular niches located within larger arteries' wall in response to hypoxic conditions. For this reason, EPCs have been regarded as an effective tool to promote revascularization and functional recovery of ischemic hearts, but clinical application failed to exploit the full potential of patients-derived cells...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28814763/spatial-genomic-heterogeneity-in-multiple-myeloma-revealed-by-multi-region-sequencing
#17
L Rasche, S S Chavan, O W Stephens, P H Patel, R Tytarenko, C Ashby, M Bauer, C Stein, S Deshpande, C Wardell, T Buzder, G Molnar, M Zangari, F van Rhee, S Thanendrarajan, C Schinke, J Epstein, F E Davies, B A Walker, T Meissner, B Barlogie, G J Morgan, N Weinhold
In multiple myeloma malignant plasma cells expand within the bone marrow. Since this site is well-perfused, a rapid dissemination of "fitter" clones may be anticipated. However, an imbalanced distribution of multiple myeloma is frequently observed in medical imaging. Here, we perform multi-region sequencing, including iliac crest and radiology-guided focal lesion specimens from 51 patients to gain insight into the spatial clonal architecture. We demonstrate spatial genomic heterogeneity in more than 75% of patients, including inactivation of CDKN2C and TP53, and mutations affecting mitogen-activated protein kinase genes...
August 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28809840/a-novel-clinical-grade-isolation-method-for-human-kidney-perivascular-stromal-cells
#18
Daniëlle G Leuning, Ellen Lievers, Marlies E J Reinders, Cees van Kooten, Marten A Engelse, Ton J Rabelink
Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential...
August 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28799229/sitagliptin-mediated-preservation-of-endothelial-progenitor-cell-function-via-augmenting-autophagy-enhances-ischaemic-angiogenesis-in-diabetes
#19
Xiaozhen Dai, Jun Zeng, Xiaoqing Yan, Qian Lin, Kai Wang, Jing Chen, Feixia Shen, Xuemei Gu, Yuehui Wang, Jun Chen, Kejian Pan, Lu Cai, Kupper A Wintergerst, Yi Tan
Recently, the dipeptidyl peptidase-4 (DPP-4) inhibitor sitagliptin, a major anti-hyperglycaemic agent, has received substantial attention as a therapeutic target for cardiovascular diseases via enhancing the number of circulating endothelial progenitor cells (EPCs). However, the direct effects of sitagliptin on EPC function remain elusive. In this study, we evaluated the proangiogenic effects of sitagliptin on a diabetic hind limb ischaemia (HLI) model in vivo and on EPC culture in vitro. Treatment of db/db mice with sitagliptin (Januvia) after HLI surgery efficiently enhanced ischaemic angiogenesis and blood perfusion, which was accompanied by significant increases in circulating EPC numbers...
August 10, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28765370/three-dimensional-biomimetic-vascular-model-reveals-a-rhoa-rac1-and-n-cadherin-balance-in-mural-cell-endothelial-cell-regulated-barrier-function
#20
Stella Alimperti, Teodelinda Mirabella, Varnica Bajaj, William Polacheck, Dana M Pirone, Jeremy Duffield, Jeroen Eyckmans, Richard K Assoian, Christopher S Chen
The integrity of the endothelial barrier between circulating blood and tissue is important for blood vessel function and, ultimately, for organ homeostasis. Here, we developed a vessel-on-a-chip with perfused endothelialized channels lined with human bone marrow stromal cells, which adopt a mural cell-like phenotype that recapitulates barrier function of the vasculature. In this model, barrier function is compromised upon exposure to inflammatory factors such as LPS, thrombin, and TNFα, as has been observed in vivo...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
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