keyword
MENU ▼
Read by QxMD icon Read
search

bone marrow perfusion

keyword
https://www.readbyqxmd.com/read/28618138/expansion-of-umbilical-cord-blood-aldehyde-dehydrogenase-expressing-cells-generates-myeloid-progenitor-cells-that-stimulate-limb-revascularization
#1
David M Putman, Tyler T Cooper, Stephen E Sherman, Ayesh K Seneviratne, Mark Hewitt, Gillian I Bell, David A Hess
Uncompromised by chronic disease-related comorbidities, human umbilical cord blood (UCB) progenitor cells with high aldehyde dehydrogenase activity (ALDH(hi) cells) stimulate blood vessel regeneration after intra-muscular transplantation. However, implementation of cellular therapies using UCB ALDH(hi) cells for critical limb ischemia, the most severe form of severe peripheral artery disease, is limited by the rarity (<0.5%) of these cells. Our goal was to generate a clinically-translatable, allogeneic cell population for vessel regenerative therapies, via ex vivo expansion of UCB ALDH(hi) cells without loss of pro-angiogenic potency...
June 15, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28616284/intrapleural-perfusion-thermo-chemotherapy-for-pleural-effusion-caused-by-lung-carcinoma-under-vats
#2
Runlei Hu, Hong Jiang, Hu Li, Dongshan Wei, Guoqing Wang, Shenglin Ma
BACKGROUND: The aim of this study was to assess the effectiveness of intrapleural perfusion thermo-chemotherapy (IPTC) under video-assisted thoracoscopic surgery (VATS) for malignant pleural effusion (MPE) caused by lung carcinoma. METHODS: In this retrospective study, fifty-four patients with moderate or large amounts of ipsilateral MPE secondary to non-small cell lung cancer (NSCLC) underwent pleural biopsy and IPTC under VATS. IPTC was performed by perfusing the pleural cavity with 43...
May 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28596576/knee-subchondral-bone-perfusion-and-its-relationship-to-marrow-fat-and-trabeculation-on-multi-parametric-mri-and-micro-ct-in-experimental-ckd
#3
Chao-Ying Wang, Yu-Juei Hsu, Yi-Jen Peng, Herng-Sheng Lee, Yue-Cune Chang, Chih-Shan Chang, Shih-Wei Chiang, Yi-Chih Hsu, Ming-Huang Lin, Guo-Shu Huang
The pathogenesis of chronic kidney disease (CKD) is multifactorial. In the progression of CKD arthropathy, arteriosclerosis may alter the knee subchondral bone marrow by altering blood flow through the bone vasculature. Herein, multi-parametric MRI assessment, including dynamic contrast enhanced magnetic resonance imaging (DCE-MRI), magnetic resonance spectroscopy (MRS), MRI T2*, contrast enhanced MR angiography (CE-MRA), and micro-CT were applied in a rodent nephrectomy model to: 1) investigate the blood perfusion of subchondral bone marrow and its relationship to fat water content and trabeculation pattern in CKD and 2) demonstrate the feasibility of using multi-parametric MRI parameters as imaging biomarkers to evaluate the disease's progression...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28594289/hypoxic-3d-cellular-network-construction-replicates-ex-vivo-the-phenotype-of-primary-human-osteocytes
#4
Saba Choudhary, Qiaoling Sun, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Young Lee
Osteocytes are deeply embedded in the mineralized matrix of bone and are non-proliferative, making them a challenge to isolate and maintain using traditional in vitro culture methods without sacrificing their inimitable phenotype. We studied the synergistic effects of two microenvironmental factors that are vital in retaining, ex vivo, the phenotype of primary human osteocytes: hypoxia and 3D cellular network. In order to recapitulate the lacunocanalicular structure of bone tissue, we assembled and cultured primary human osteocytic cells with biphasic calcium phosphate microbeads in a microfluidic perfusion culture device...
June 8, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28570033/quantitative-mri-and-spectroscopy-of-bone-marrow
#5
REVIEW
Dimitrios C Karampinos, Stefan Ruschke, Michael Dieckmeyer, Maximilian Diefenbach, Daniela Franz, Alexandra S Gersing, Roland Krug, Thomas Baum
Bone marrow is one of the largest organs in the human body, enclosing adipocytes, hematopoietic stem cells, which are responsible for blood cell production, and mesenchymal stem cells, which are responsible for the production of adipocytes and bone cells. Magnetic resonance imaging (MRI) is the ideal imaging modality to monitor bone marrow changes in healthy and pathological states, thanks to its inherent rich soft-tissue contrast. Quantitative bone marrow MRI and magnetic resonance spectroscopy (MRS) techniques have been also developed in order to quantify changes in bone marrow water-fat composition, cellularity and perfusion in different pathologies, and to assist in understanding the role of bone marrow in the pathophysiology of systemic diseases (e...
June 1, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28535778/exosomes-from-mesenchymal-stem-cells-induce-the-conversion-of-hepatocytes-into-progenitor-oval-cells
#6
Hao-Hsiang Wu, Oscar K Lee
BACKGROUND: We previously reported that mesenchymal stem cells (MSCs) possess therapeutic effects in a murine model of carbon tetrachloride-induced acute liver failure. In the study, we observed that the majority of repopulated hepatocytes were of recipient origin and were adjacent to transplanted MSCs; only a low percentage of repopulated hepatocytes were from transplanted MSCs. The findings indicate that MSCs guided the formation of new hepatocytes. Exosomes are important messengers for paracrine signaling delivery...
May 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28529639/overexpression-of-insulin-like-growth-factor-1-enhanced-the-osteogenic-capability-of-aging-bone-marrow-mesenchymal-stem-cells
#7
Ching-Yun Chen, Kuo-Yun Tseng, Yen-Liang Lai, Yo-Shen Chen, Feng-Huei Lin, Shankung Lin
Many studies have indicated that loss of the osteoblastogenic potential in bone marrow mesenchymal stem cells (bmMSCs) is the major component in the etiology of the aging-related bone deficit. But how the bmMSCs lose osteogenic capability in aging is unclear. Using 2-dimentional cultures, we examined the dose response of human bmMSCs, isolated from adult and aged donors, to exogenous insulin-like growth factor 1 (IGF-1), a growth factor regulating bone formation. The data showed that the mitogenic activity and the osteoblastogenic potential of bmMSCs in response to IGF-1 were impaired with aging, whereas higher doses of IGF-1 increased the proliferation rate and osteogenic potential of aging bmMSCs...
2017: Theranostics
https://www.readbyqxmd.com/read/28524375/bioprinted-osteogenic-and-vasculogenic-patterns-for-engineering-3d-bone-tissue
#8
Batzaya Byambaa, Nasim Annabi, Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Weitao Jia, Mehdi Kazemzadeh-Narbat, Su Ryon Shin, Ali Tamayol, Ali Khademhosseini
Fabricating 3D large-scale bone tissue constructs with functional vasculature has been a particular challenge in engineering tissues suitable for repairing large bone defects. To address this challenge, an extrusion-based direct-writing bioprinting strategy is utilized to fabricate microstructured bone-like tissue constructs containing a perfusable vascular lumen. The bioprinted constructs are used as biomimetic in vitro matrices to co-culture human umbilical vein endothelial cells and bone marrow derived human mesenchymal stem cells in a naturally derived hydrogel...
May 19, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28451811/prospective-clinical-and-pharmacological-evaluation-of-the-delcath-system-s-second-generation-gen2-hemofiltration-system-in-patients-undergoing-percutaneous-hepatic-perfusion-with-melphalan
#9
Eleonora M de Leede, Mark C Burgmans, T Susanna Meijer, Christian H Martini, Fred G J Tijl, Jaap Vuyk, Arian R van Erkel, Cornelis J H van der Velde, Ellen Kapiteijn, Alexander L Vahrmeijer
INTRODUCTION: Percutaneous hepatic perfusion (PHP) with melphalan is an effective treatment for patients with hepatic metastases, but associated with high rates of bone marrow depression. To reduce systemic toxicity, improvements have been made to the filtration system. In pre-clinical studies, the Delcath System's GEN2 filter was superior to the first-generation filters. In this clinical study, we analysed the pharmacokinetics and toxicity of PHP using the new GEN2 filter. METHODS AND MATERIALS: Starting February 2014, two prospective phase II studies were initiated in patients with hepatic metastases from ocular melanoma or colorectal cancer...
April 27, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28408376/a-comparative-study-of-tissue-engineered-constructs-from-acropora-and-porites-coral-in-a-large-animal-bone-defect-model
#10
A Decambron, M Manassero, M Bensidhoum, B Lecuelle, D Logeart-Avramoglou, H Petite, V Viateau
OBJECTIVES: To compare the therapeutic potential of tissue-engineered constructs (TECs) combining mesenchymal stem cells (MSCs) and coral granules from either Acropora or Porites to repair large bone defects. MATERIALS AND METHODS: Bone marrow-derived, autologous MSCs were seeded on Acropora or Porites coral granules in a perfusion bioreactor. Acropora-TECs (n = 7), Porites-TECs (n = 6) and bone autografts (n = 2) were then implanted into 25 mm long metatarsal diaphyseal defects in sheep...
April 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28374977/hypoxic-preconditioning-induced-autophagy-enhances-survival-of-engrafted-endothelial-progenitor-cells-in-ischaemic-limb
#11
Pei Zhou, Yu-Zhen Tan, Hai-Jie Wang, Guo-Dong Wang
Recent clinical studies have suggested that endothelial progenitor cells (EPCs) transplantation provides a modest benefit for treatment of the ischaemic diseases such as limb ischaemia. However, cell-based therapies have been limited by poor survival of the engrafted cells. This investigation was designed to establish optimal hypoxia preconditioning and evaluate effects of hypoxic preconditioning-induced autophagy on survival of the engrafted EPCs. Autophagy of CD34(+) VEGFR-2(+) EPCs isolated from rat bone marrow increased after treatment with 1% O2 ...
April 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28374486/a-novel-cxcr4-antagonist-enhances-angiogenesis-via-modifying-the-ischaemic-tissue-environment
#12
Xiaoqing Yan, Xiaozhen Dai, Luqing He, Xiao Ling, Minglong Shao, Chi Zhang, Yuehui Wang, Jian Xiao, Lu Cai, Xiaokun Li, Yi Tan
Endothelial progenitor cells (EPCs) play a capital role in angiogenesis via directly participating in neo-vessel formation and secreting pro-angiogenic factors. Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4 play a critical role in the retention and quiescence of EPCs within its niche in the bone marrow. Disturbing the interaction between SDF-1 and CXCR4 is an effective strategy for EPC mobilization. We developed a novel CXCR4 antagonist P2G, a mutant protein of SDF-1β with high antagonistic activity against CXCR4 and high potency in enhancing ischaemic angiogenesis and blood perfusion...
April 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28356443/a-microrna93-irf9-irg1-itaconic-acid-pathway-modulates-m2-like-macrophage-polarization-to-revascularize-ischemic-muscle
#13
Vijay Chaitanya Ganta, Min Hyub Choi, Anna Kutateladze, Todd E Fox, Charles R Farber, Brian H Annex
Background -Currently no therapies exist for treating, and improving outcomes in patients with severe peripheral arterial disease (PAD). MicroRNA93 (miR93) has been shown to favorably modulate angiogenesis and reduce tissue loss in genetic PAD models. However, the cell specific function, downstream mechanisms or signaling involved in miR93 mediated ischemic muscle neovascularization is not clear. Macrophages were best known to modulate arteriogenic response in PAD and the extent of arteriogenic response induced by macrophages is dependent on greater M2 to M1-activation/polarization state...
March 29, 2017: Circulation
https://www.readbyqxmd.com/read/28351209/effects-of-multiple-injection-of-bone-marrow-mononuclear-cells-on-spinal-cord-injury-of-rats
#14
Kenji Kanekiyo, Norihiko Nakano, Tamami Homma, Yoshihiro Yamada, Masahiro Tamachi, Yoshihisa Suzuki, Masanori Fukushima, Fukuki Saito, Chizuka Ide
The effects of multiple-injection of bone marrow mononuclear cells (BMNCs) on spinal cord injury (SCI) were compared with those of single-injection in rats. BMNCs separated by density-gradient centrifugation from a bone marrow perfusate were injected 3 times (once weekly) through the cerebrospinal fluid (CSF) via the 4th ventricle, and the locomotor improvement and tissue recovery, including axonal regeneration, were compared with those of single-injection. While the single-injection group showed a steep elevation of the BBB (Basso-Beattie-Bresnahan) score 1 week after transplantation, the multiple-injection group maintained a similar steep elevation for 2 weeks after transplantation, and the BBB scores of the multiple-injection group remained thereafter at a level around 2-3 points higher than those of the single-injection group until the end of the experiment...
March 28, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28347909/bone-marrow-perfusion-measured-with-dynamic-contrast-enhanced-magnetic-resonance-imaging-is-correlated-to-body-mass-index-in-adults
#15
Jean-François Budzik, Guillaume Lefebvre, Hélène Behal, Sébastien Verclytte, Pierre Hardouin, Pedro Teixeira, Anne Cotten
Bone marrow metabolism is complex and far from being fully understood. Novel aspects, such as the roles of bone marrow adiposity and vascularisation in bone metabolism currently attract attention. There is also a growing interest in the influence obesity might have on bone metabolism. Our objective was to determine the effect of BMI on bone marrow perfusion parameters using dynamic contrast-enhanced magnetic resonance imaging. This prospective monocentric study was approved by our local Ethics committee. Written consent was obtained...
March 24, 2017: Bone
https://www.readbyqxmd.com/read/28295901/high-aldehyde-dehydrogenase-activity-identifies-a-subset-of-human-mesenchymal-stromal-cells-with-vascular-regenerative-potential
#16
Stephen E Sherman, Miljan Kuljanin, Tyler T Cooper, David M Putman, Gilles A Lajoie, David A Hess
During culture expansion, multipotent mesenchymal stromal cells (MSCs) differentially express aldehyde dehydrogenase (ALDH), an intracellular detoxification enzyme that protects long-lived cells against oxidative stress. Thus, MSC selection based on ALDH-activity may be used to reduce heterogeneity and distinguish MSC subsets with improved regenerative potency. After expansion of human bone marrow-derived MSCs, cell progeny was purified based on low versus high ALDH-activity (ALDH(hi) ) by fluorescence-activated cell sorting, and each subset was compared for multipotent stromal and provascular regenerative functions...
March 15, 2017: Stem Cells
https://www.readbyqxmd.com/read/28265697/intravoxel-incoherent-motion-diffusion-weighted-magnetic-resonance-imaging-of-focal-vertebral-bone-marrow-lesions-initial-experience-of-the-differentiation-of-nodular-hyperplastic-hematopoietic-bone-marrow-from-malignant-lesions
#17
Sunghoon Park, Kyu-Sung Kwack, Nam-Su Chung, Jinwoo Hwang, Hyun Young Lee, Jae Ho Kim
OBJECTIVE: To evaluate the ability of intravoxel incoherent motion (IVIM) diffusion-weighted magnetic resonance imaging (MRI) parameters to differentiate nodular hyperplastic hematopoietic bone marrow (HHBM) from malignant vertebral bone marrow lesions (VBMLs). MATERIALS AND METHODS: A total of 33 patients with 58 VBMLs, including 9 nodular HHBM lesions, 39 bone metastases, and 10 myelomas, were retrospectively assessed. All diagnoses were confirmed either pathologically or via image assessment...
March 6, 2017: Skeletal Radiology
https://www.readbyqxmd.com/read/28225579/intravoxel-incoherent-motion-mri-assessment-of-chemoradiation-induced-pelvic-bone-marrow-changes-in-cervical-cancer-and-correlation-with-hematological-toxicity
#18
Elaine Yuen Phin Lee, Jose Angelo Udal Perucho, Vince Vardhanabhuti, Jian He, Steven Wai Kwan Siu, Siew Fei Ngu, Nina A Mayr, William T C Yuh, Queenie Chan, Pek-Lan Khong
PURPOSE: To investigate bone marrow changes after chemoradiation (CRT) using intravoxel incoherent motion magnetic resonance imaging (IVIM-MRI) and correlate imaging changes with hematological toxicity (HT) in patients with locally advanced cervical cancer. MATERIALS AND METHODS: Thirty-nine patients with newly diagnosed cervical cancer were prospectively recruited for two sequential 3.0T IVIM-MRI studies: before treatment (MRI-1) and 3-4 weeks after standardized CRT (MRI-2)...
February 22, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28216452/water-and-lipid-diffusion-mri-using-chemical-shift-displacement-based-separation-of-lipid-tissue-split
#19
Naoki Ohno, Hirohito Kan, Tosiaki Miyati, Toshitaka Aoki, Shota Ishida, Toshifumi Gabata
PURPOSE: To obtain water and lipid diffusion-weighted images (DWIs) simultaneously, we devised a novel method utilizing chemical shift displacement-based separation of lipid tissue (SPLIT) imaging. MATERIALS AND METHODS: Single-shot diffusion echo-planar imaging without fat suppression was used and the imaging parameters were optimized to separate water and lipid DWIs by chemical shift displacement of the lipid signals along the phase-encoding direction. Using the optimized conditions, transverse DWIs at the maximum diameter of the right calf were scanned with multiple b-values in five healthy subjects...
June 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28211619/intravoxel-incoherent-motion-diffusion-weighted-imaging-of-bone-marrow-in-patients-with-acute-myeloid-leukemia-a-pilot-study-of-prognostic-value
#20
Jinliang Niu, Wenjin Li, Hongwei Wang, Wenqi Wu, Tong Gong, Ning Huang, Jun Wang, Yan Qi
PURPOSE: To investigate the value of intravoxel incoherent motion (IVIM) parameters in evaluation of prognosis in patients with acute myeloid leukemia (AML) before treatment. MATERIALS AND METHODS: Fifty-three patients before standard chemotherapy underwent MRI scans at 1.5 Tesla using conventional diffusion weighted imaging (DWI) and IVIM (b = 0, 10, 25, 50, 100, 200, 400, 600, 800, 1000, 1200 s/mm(2) ) in the sagittal plane covering the lumbar bone marrow...
February 17, 2017: Journal of Magnetic Resonance Imaging: JMRI
keyword
keyword
64554
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"